CINXE.COM

<!DOCTYPE html> <html lang="en-US"> <head > <meta charset="UTF-8" /> <meta name="viewport" content="width=device-width, initial-scale=1" /> <meta name='robots' content='index, follow, max-image-preview:large, max-snippet:-1, max-video-preview:-1' />  <title>Improving Agave Syrup Carbohydrate Analysis With Automatic Eluent Generation</title> <meta name="description" content="The increased popularity of agave syrup means that inauthentic variations are entering circulation, requiring greater analysis." /> <link rel="canonical" href="https://www.thermofisher.com/blog/analyteguru/improving-agave-syrup-carbohydrate-analysis-with-automatic/" /> <meta property="og:locale" content="en_US" /> <meta property="og:type" content="article" /> <meta property="og:title" content="Improving Agave Syrup Carbohydrate Analysis With Automatic Eluent Generation" /> <meta property="og:description" content="The increased popularity of agave syrup means that inauthentic variations are entering circulation, requiring greater analysis." /> <meta property="og:url" content="https://www.thermofisher.com/blog/analyteguru/improving-agave-syrup-carbohydrate-analysis-with-automatic/" /> <meta property="og:site_name" content="AnalyteGuru" /> <meta property="article:publisher" content="https://www.facebook.com/ChromatographySolutions" /> <meta property="article:published_time" content="2022-10-24T14:46:58+00:00" /> <meta property="article:modified_time" content="2024-03-01T19:39:46+00:00" /> <meta property="og:image" content="https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2023/06/19075-og.jpg" /> <meta property="og:image:width" content="998" /> <meta property="og:image:height" content="661" /> <meta property="og:image:type" content="image/jpeg" /> <meta name="author" content="AnalyteGuru Staff" /> <meta name="twitter:card" content="summary_large_image" /> <meta name="twitter:creator" content="@ChromSolutions" /> <meta name="twitter:site" content="@ChromSolutions" /> <meta name="twitter:label1" content="Written by" /> <meta name="twitter:data1" content="AnalyteGuru Staff" /> <meta name="twitter:label2" content="Est. reading time" /> <meta name="twitter:data2" content="5 minutes" /> <script type="application/ld+json" class="yoast-schema-graph">{"@context":"https://schema.org","@graph":[{"@type":"WebPage","@id":"https://www.thermofisher.com/blog/analyteguru/improving-agave-syrup-carbohydrate-analysis-with-automatic/","url":"https://www.thermofisher.com/blog/analyteguru/improving-agave-syrup-carbohydrate-analysis-with-automatic/","name":"Improving Agave Syrup Carbohydrate Analysis With Automatic Eluent Generation","isPartOf":{"@id":"https://www.thermofisher.com/blog/analyteguru/#website"},"primaryImageOfPage":{"@id":"https://www.thermofisher.com/blog/analyteguru/improving-agave-syrup-carbohydrate-analysis-with-automatic/#primaryimage"},"image":{"@id":"https://www.thermofisher.com/blog/analyteguru/improving-agave-syrup-carbohydrate-analysis-with-automatic/#primaryimage"},"thumbnailUrl":"https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2023/06/19075-og.jpg","datePublished":"2022-10-24T14:46:58+00:00","dateModified":"2024-03-01T19:39:46+00:00","author":{"@id":"https://www.thermofisher.com/blog/analyteguru/#/schema/person/47226155467d66d692f24162ee320270"},"description":"The increased popularity of agave syrup means that inauthentic variations are entering circulation, requiring greater analysis.","breadcrumb":{"@id":"https://www.thermofisher.com/blog/analyteguru/improving-agave-syrup-carbohydrate-analysis-with-automatic/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https://www.thermofisher.com/blog/analyteguru/improving-agave-syrup-carbohydrate-analysis-with-automatic/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https://www.thermofisher.com/blog/analyteguru/improving-agave-syrup-carbohydrate-analysis-with-automatic/#primaryimage","url":"https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2023/06/19075-og.jpg","contentUrl":"https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2023/06/19075-og.jpg","width":998,"height":661},{"@type":"BreadcrumbList","@id":"https://www.thermofisher.com/blog/analyteguru/improving-agave-syrup-carbohydrate-analysis-with-automatic/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https://www.thermofisher.com/blog/analyteguru/"},{"@type":"ListItem","position":2,"name":"Improving Agave Syrup Carbohydrate Analysis With Automatic Eluent Generation"}]},{"@type":"WebSite","@id":"https://www.thermofisher.com/blog/analyteguru/#website","url":"https://www.thermofisher.com/blog/analyteguru/","name":"AnalyteGuru","description":"Your educational resource for biopharma, pharma, environmental, food and agriculture, industrial, and clinical labs","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https://www.thermofisher.com/blog/analyteguru/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Person","@id":"https://www.thermofisher.com/blog/analyteguru/#/schema/person/47226155467d66d692f24162ee320270","name":"AnalyteGuru Staff","image":{"@type":"ImageObject","inLanguage":"en-US","@id":"https://www.thermofisher.com/blog/analyteguru/#/schema/person/image/","url":"https://secure.gravatar.com/avatar/13dc4fe276cbef4815beab3822073707?s=96&d=mm&r=g","contentUrl":"https://secure.gravatar.com/avatar/13dc4fe276cbef4815beab3822073707?s=96&d=mm&r=g","caption":"AnalyteGuru Staff"},"url":"https://www.thermofisher.com/blog/author/kerrycolligan/"}]}</script>  <link rel='dns-prefetch' href='//www.thermofisher.com' /> <link rel='dns-prefetch' href='//players.brightcove.net' /> <link rel='dns-prefetch' href='//use.fontawesome.com' /> <link rel="alternate" type="application/rss+xml" title="AnalyteGuru » Feed" href="https://www.thermofisher.com/blog/analyteguru/feed/" /> <link rel="alternate" type="application/rss+xml" title="AnalyteGuru » Comments Feed" href="https://www.thermofisher.com/blog/analyteguru/comments/feed/" /> <link rel="alternate" type="application/rss+xml" title="AnalyteGuru » Improving Agave Syrup Carbohydrate Analysis With Automatic Eluent Generation Comments Feed" href="https://www.thermofisher.com/blog/analyteguru/improving-agave-syrup-carbohydrate-analysis-with-automatic/feed/" />  <script src="https://cdn.optimizely.com/js/8156647427.js"></script>  <script> (function(h,o,t,j,a,r){ h.hj=h.hj||function(){(h.hj.q=h.hj.q||[]).push(arguments)}; h._hjSettings={hjid:373708,hjsv:5}; a=o.getElementsByTagName('head')[0]; r=o.createElement('script');r.async=1; r.src=t+h._hjSettings.hjid+j+h._hjSettings.hjsv; a.appendChild(r); })(window,document,'//static.hotjar.com/c/hotjar-','.js?sv='); </script>  <script src="//www.googletagmanager.com/gtag/js?id=G-TJ18KZWFVE" data-cfasync="false" data-wpfc-render="false" type="text/javascript" async></script> <script data-cfasync="false" data-wpfc-render="false" type="text/javascript"> var mi_version = '9.2.0'; var mi_track_user = true; var mi_no_track_reason = ''; var MonsterInsightsDefaultLocations = {"page_location":"https:\/\/admin.acceleratingscience.com\/analyteguru\/improving-agave-syrup-carbohydrate-analysis-with-automatic\/"}; if ( typeof MonsterInsightsPrivacyGuardFilter === 'function' ) { var MonsterInsightsLocations = (typeof MonsterInsightsExcludeQuery === 'object') ? MonsterInsightsPrivacyGuardFilter( MonsterInsightsExcludeQuery ) : MonsterInsightsPrivacyGuardFilter( MonsterInsightsDefaultLocations ); } else { var MonsterInsightsLocations = (typeof MonsterInsightsExcludeQuery === 'object') ? MonsterInsightsExcludeQuery : MonsterInsightsDefaultLocations; } var disableStrs = [ 'ga-disable-G-TJ18KZWFVE', ]; /* Function to detect opted out users */ function __gtagTrackerIsOptedOut() { for (var index = 0; index < disableStrs.length; index++) { if (document.cookie.indexOf(disableStrs[index] + '=true') > -1) { return true; } } return false; } /* Disable tracking if the opt-out cookie exists. */ if (__gtagTrackerIsOptedOut()) { for (var index = 0; index < disableStrs.length; index++) { window[disableStrs[index]] = true; } } /* Opt-out function */ function __gtagTrackerOptout() { for (var index = 0; index < disableStrs.length; index++) { document.cookie = disableStrs[index] + '=true; expires=Thu, 31 Dec 2099 23:59:59 UTC; path=/'; window[disableStrs[index]] = true; } } if ('undefined' === typeof gaOptout) { function gaOptout() { __gtagTrackerOptout(); } } window.dataLayer = window.dataLayer || []; window.MonsterInsightsDualTracker = { helpers: {}, trackers: {}, }; if (mi_track_user) { function __gtagDataLayer() { dataLayer.push(arguments); } function __gtagTracker(type, name, parameters) { if (!parameters) { parameters = {}; } if (parameters.send_to) { __gtagDataLayer.apply(null, arguments); return; } if (type === 'event') { parameters.send_to = monsterinsights_frontend.v4_id; var hookName = name; if (typeof parameters['event_category'] !== 'undefined') { hookName = parameters['event_category'] + ':' + name; } if (typeof MonsterInsightsDualTracker.trackers[hookName] !== 'undefined') { MonsterInsightsDualTracker.trackers[hookName](parameters); } else { __gtagDataLayer('event', name, parameters); } } else { __gtagDataLayer.apply(null, arguments); } } __gtagTracker('js', new Date()); __gtagTracker('set', { 'developer_id.dZGIzZG': true, }); if ( MonsterInsightsLocations.page_location ) { __gtagTracker('set', MonsterInsightsLocations); } __gtagTracker('config', 'G-TJ18KZWFVE', {"use_amp_client_id":"true","forceSSL":"true","link_attribution":"true"} ); window.gtag = __gtagTracker; (function () { /* https://developers.google.com/analytics/devguides/collection/analyticsjs/ */ /* ga and __gaTracker compatibility shim. */ var noopfn = function () { return null; }; var newtracker = function () { return new Tracker(); }; var Tracker = function () { return null; }; var p = Tracker.prototype; p.get = noopfn; p.set = noopfn; p.send = function () { var args = Array.prototype.slice.call(arguments); args.unshift('send'); __gaTracker.apply(null, args); }; var __gaTracker = function () { var len = arguments.length; if (len === 0) { return; } var f = arguments[len - 1]; if (typeof f !== 'object' || f === null || typeof f.hitCallback !== 'function') { if ('send' === arguments[0]) { var hitConverted, hitObject = false, action; if ('event' === arguments[1]) { if ('undefined' !== typeof arguments[3]) { hitObject = { 'eventAction': arguments[3], 'eventCategory': arguments[2], 'eventLabel': arguments[4], 'value': arguments[5] ? arguments[5] : 1, } } } if ('pageview' === arguments[1]) { if ('undefined' !== typeof arguments[2]) { hitObject = { 'eventAction': 'page_view', 'page_path': arguments[2], } } } if (typeof arguments[2] === 'object') { hitObject = arguments[2]; } if (typeof arguments[5] === 'object') { Object.assign(hitObject, arguments[5]); } if ('undefined' !== typeof arguments[1].hitType) { hitObject = arguments[1]; if ('pageview' === hitObject.hitType) { hitObject.eventAction = 'page_view'; } } if (hitObject) { action = 'timing' === arguments[1].hitType ? 'timing_complete' : hitObject.eventAction; hitConverted = mapArgs(hitObject); __gtagTracker('event', action, hitConverted); } } return; } function mapArgs(args) { var arg, hit = {}; var gaMap = { 'eventCategory': 'event_category', 'eventAction': 'event_action', 'eventLabel': 'event_label', 'eventValue': 'event_value', 'nonInteraction': 'non_interaction', 'timingCategory': 'event_category', 'timingVar': 'name', 'timingValue': 'value', 'timingLabel': 'event_label', 'page': 'page_path', 'location': 'page_location', 'title': 'page_title', 'referrer' : 'page_referrer', }; for (arg in args) { if (!(!args.hasOwnProperty(arg) || !gaMap.hasOwnProperty(arg))) { hit[gaMap[arg]] = args[arg]; } else { hit[arg] = args[arg]; } } return hit; } try { f.hitCallback(); } catch (ex) { } }; __gaTracker.create = newtracker; __gaTracker.getByName = newtracker; __gaTracker.getAll = function () { return []; }; __gaTracker.remove = noopfn; __gaTracker.loaded = true; window['__gaTracker'] = __gaTracker; })(); } else { console.log(""); (function () { function __gtagTracker() { return null; } window['__gtagTracker'] = __gtagTracker; window['gtag'] = __gtagTracker; })(); } </script>  <link rel='stylesheet' id='accelerating-science-blogs-css' href='https://www.thermofisher.com/blog/analyteguru/wp-content/themes/accelerating-science-multilingual-blogs/style.css?ver=1.2.9.3' type='text/css' media='all' /> <link rel='stylesheet' id='wp-block-library-css' href='https://www.thermofisher.com/blog//wp-includes/css/dist/block-library/style.min.css?ver=6.6.1' type='text/css' media='all' /> <style id='classic-theme-styles-inline-css' type='text/css'> /*! This file is auto-generated */ .wp-block-button__link{color:#fff;background-color:#32373c;border-radius:9999px;box-shadow:none;text-decoration:none;padding:calc(.667em + 2px) calc(1.333em + 2px);font-size:1.125em}.wp-block-file__button{background:#32373c;color:#fff;text-decoration:none} </style> <style id='global-styles-inline-css' type='text/css'> :root{--wp--preset--aspect-ratio--square: 1;--wp--preset--aspect-ratio--4-3: 4/3;--wp--preset--aspect-ratio--3-4: 3/4;--wp--preset--aspect-ratio--3-2: 3/2;--wp--preset--aspect-ratio--2-3: 2/3;--wp--preset--aspect-ratio--16-9: 16/9;--wp--preset--aspect-ratio--9-16: 9/16;--wp--preset--color--black: #000000;--wp--preset--color--cyan-bluish-gray: #abb8c3;--wp--preset--color--white: #ffffff;--wp--preset--color--pale-pink: #f78da7;--wp--preset--color--vivid-red: #cf2e2e;--wp--preset--color--luminous-vivid-orange: #ff6900;--wp--preset--color--luminous-vivid-amber: #fcb900;--wp--preset--color--light-green-cyan: #7bdcb5;--wp--preset--color--vivid-green-cyan: #00d084;--wp--preset--color--pale-cyan-blue: #8ed1fc;--wp--preset--color--vivid-cyan-blue: #0693e3;--wp--preset--color--vivid-purple: #9b51e0;--wp--preset--gradient--vivid-cyan-blue-to-vivid-purple: linear-gradient(135deg,rgba(6,147,227,1) 0%,rgb(155,81,224) 100%);--wp--preset--gradient--light-green-cyan-to-vivid-green-cyan: linear-gradient(135deg,rgb(122,220,180) 0%,rgb(0,208,130) 100%);--wp--preset--gradient--luminous-vivid-amber-to-luminous-vivid-orange: linear-gradient(135deg,rgba(252,185,0,1) 0%,rgba(255,105,0,1) 100%);--wp--preset--gradient--luminous-vivid-orange-to-vivid-red: linear-gradient(135deg,rgba(255,105,0,1) 0%,rgb(207,46,46) 100%);--wp--preset--gradient--very-light-gray-to-cyan-bluish-gray: linear-gradient(135deg,rgb(238,238,238) 0%,rgb(169,184,195) 100%);--wp--preset--gradient--cool-to-warm-spectrum: linear-gradient(135deg,rgb(74,234,220) 0%,rgb(151,120,209) 20%,rgb(207,42,186) 40%,rgb(238,44,130) 60%,rgb(251,105,98) 80%,rgb(254,248,76) 100%);--wp--preset--gradient--blush-light-purple: linear-gradient(135deg,rgb(255,206,236) 0%,rgb(152,150,240) 100%);--wp--preset--gradient--blush-bordeaux: linear-gradient(135deg,rgb(254,205,165) 0%,rgb(254,45,45) 50%,rgb(107,0,62) 100%);--wp--preset--gradient--luminous-dusk: linear-gradient(135deg,rgb(255,203,112) 0%,rgb(199,81,192) 50%,rgb(65,88,208) 100%);--wp--preset--gradient--pale-ocean: linear-gradient(135deg,rgb(255,245,203) 0%,rgb(182,227,212) 50%,rgb(51,167,181) 100%);--wp--preset--gradient--electric-grass: linear-gradient(135deg,rgb(202,248,128) 0%,rgb(113,206,126) 100%);--wp--preset--gradient--midnight: linear-gradient(135deg,rgb(2,3,129) 0%,rgb(40,116,252) 100%);--wp--preset--font-size--small: 13px;--wp--preset--font-size--medium: 20px;--wp--preset--font-size--large: 36px;--wp--preset--font-size--x-large: 42px;--wp--preset--spacing--20: 0.44rem;--wp--preset--spacing--30: 0.67rem;--wp--preset--spacing--40: 1rem;--wp--preset--spacing--50: 1.5rem;--wp--preset--spacing--60: 2.25rem;--wp--preset--spacing--70: 3.38rem;--wp--preset--spacing--80: 5.06rem;--wp--preset--shadow--natural: 6px 6px 9px rgba(0, 0, 0, 0.2);--wp--preset--shadow--deep: 12px 12px 50px rgba(0, 0, 0, 0.4);--wp--preset--shadow--sharp: 6px 6px 0px rgba(0, 0, 0, 0.2);--wp--preset--shadow--outlined: 6px 6px 0px -3px rgba(255, 255, 255, 1), 6px 6px rgba(0, 0, 0, 1);--wp--preset--shadow--crisp: 6px 6px 0px rgba(0, 0, 0, 1);}:where(.is-layout-flex){gap: 0.5em;}:where(.is-layout-grid){gap: 0.5em;}body .is-layout-flex{display: flex;}.is-layout-flex{flex-wrap: wrap;align-items: center;}.is-layout-flex > :is(*, div){margin: 0;}body .is-layout-grid{display: grid;}.is-layout-grid > :is(*, div){margin: 0;}:where(.wp-block-columns.is-layout-flex){gap: 2em;}:where(.wp-block-columns.is-layout-grid){gap: 2em;}:where(.wp-block-post-template.is-layout-flex){gap: 1.25em;}:where(.wp-block-post-template.is-layout-grid){gap: 1.25em;}.has-black-color{color: var(--wp--preset--color--black) !important;}.has-cyan-bluish-gray-color{color: var(--wp--preset--color--cyan-bluish-gray) !important;}.has-white-color{color: var(--wp--preset--color--white) !important;}.has-pale-pink-color{color: var(--wp--preset--color--pale-pink) !important;}.has-vivid-red-color{color: var(--wp--preset--color--vivid-red) !important;}.has-luminous-vivid-orange-color{color: var(--wp--preset--color--luminous-vivid-orange) !important;}.has-luminous-vivid-amber-color{color: var(--wp--preset--color--luminous-vivid-amber) !important;}.has-light-green-cyan-color{color: var(--wp--preset--color--light-green-cyan) !important;}.has-vivid-green-cyan-color{color: var(--wp--preset--color--vivid-green-cyan) !important;}.has-pale-cyan-blue-color{color: var(--wp--preset--color--pale-cyan-blue) !important;}.has-vivid-cyan-blue-color{color: var(--wp--preset--color--vivid-cyan-blue) !important;}.has-vivid-purple-color{color: var(--wp--preset--color--vivid-purple) !important;}.has-black-background-color{background-color: var(--wp--preset--color--black) !important;}.has-cyan-bluish-gray-background-color{background-color: var(--wp--preset--color--cyan-bluish-gray) !important;}.has-white-background-color{background-color: var(--wp--preset--color--white) !important;}.has-pale-pink-background-color{background-color: var(--wp--preset--color--pale-pink) !important;}.has-vivid-red-background-color{background-color: var(--wp--preset--color--vivid-red) !important;}.has-luminous-vivid-orange-background-color{background-color: var(--wp--preset--color--luminous-vivid-orange) !important;}.has-luminous-vivid-amber-background-color{background-color: var(--wp--preset--color--luminous-vivid-amber) !important;}.has-light-green-cyan-background-color{background-color: var(--wp--preset--color--light-green-cyan) !important;}.has-vivid-green-cyan-background-color{background-color: var(--wp--preset--color--vivid-green-cyan) !important;}.has-pale-cyan-blue-background-color{background-color: var(--wp--preset--color--pale-cyan-blue) !important;}.has-vivid-cyan-blue-background-color{background-color: var(--wp--preset--color--vivid-cyan-blue) !important;}.has-vivid-purple-background-color{background-color: var(--wp--preset--color--vivid-purple) !important;}.has-black-border-color{border-color: var(--wp--preset--color--black) !important;}.has-cyan-bluish-gray-border-color{border-color: var(--wp--preset--color--cyan-bluish-gray) !important;}.has-white-border-color{border-color: var(--wp--preset--color--white) !important;}.has-pale-pink-border-color{border-color: var(--wp--preset--color--pale-pink) !important;}.has-vivid-red-border-color{border-color: var(--wp--preset--color--vivid-red) !important;}.has-luminous-vivid-orange-border-color{border-color: var(--wp--preset--color--luminous-vivid-orange) !important;}.has-luminous-vivid-amber-border-color{border-color: var(--wp--preset--color--luminous-vivid-amber) !important;}.has-light-green-cyan-border-color{border-color: var(--wp--preset--color--light-green-cyan) !important;}.has-vivid-green-cyan-border-color{border-color: var(--wp--preset--color--vivid-green-cyan) !important;}.has-pale-cyan-blue-border-color{border-color: var(--wp--preset--color--pale-cyan-blue) !important;}.has-vivid-cyan-blue-border-color{border-color: var(--wp--preset--color--vivid-cyan-blue) !important;}.has-vivid-purple-border-color{border-color: var(--wp--preset--color--vivid-purple) !important;}.has-vivid-cyan-blue-to-vivid-purple-gradient-background{background: var(--wp--preset--gradient--vivid-cyan-blue-to-vivid-purple) !important;}.has-light-green-cyan-to-vivid-green-cyan-gradient-background{background: var(--wp--preset--gradient--light-green-cyan-to-vivid-green-cyan) !important;}.has-luminous-vivid-amber-to-luminous-vivid-orange-gradient-background{background: var(--wp--preset--gradient--luminous-vivid-amber-to-luminous-vivid-orange) !important;}.has-luminous-vivid-orange-to-vivid-red-gradient-background{background: var(--wp--preset--gradient--luminous-vivid-orange-to-vivid-red) !important;}.has-very-light-gray-to-cyan-bluish-gray-gradient-background{background: var(--wp--preset--gradient--very-light-gray-to-cyan-bluish-gray) !important;}.has-cool-to-warm-spectrum-gradient-background{background: var(--wp--preset--gradient--cool-to-warm-spectrum) !important;}.has-blush-light-purple-gradient-background{background: var(--wp--preset--gradient--blush-light-purple) !important;}.has-blush-bordeaux-gradient-background{background: var(--wp--preset--gradient--blush-bordeaux) !important;}.has-luminous-dusk-gradient-background{background: var(--wp--preset--gradient--luminous-dusk) !important;}.has-pale-ocean-gradient-background{background: var(--wp--preset--gradient--pale-ocean) !important;}.has-electric-grass-gradient-background{background: var(--wp--preset--gradient--electric-grass) !important;}.has-midnight-gradient-background{background: var(--wp--preset--gradient--midnight) !important;}.has-small-font-size{font-size: var(--wp--preset--font-size--small) !important;}.has-medium-font-size{font-size: var(--wp--preset--font-size--medium) !important;}.has-large-font-size{font-size: var(--wp--preset--font-size--large) !important;}.has-x-large-font-size{font-size: var(--wp--preset--font-size--x-large) !important;} :where(.wp-block-post-template.is-layout-flex){gap: 1.25em;}:where(.wp-block-post-template.is-layout-grid){gap: 1.25em;} :where(.wp-block-columns.is-layout-flex){gap: 2em;}:where(.wp-block-columns.is-layout-grid){gap: 2em;} :root :where(.wp-block-pullquote){font-size: 1.5em;line-height: 1.6;} </style> <link rel='stylesheet' id='gforms_reset_css-css' href='https://www.thermofisher.com/blog/analyteguru/wp-content/plugins/gravityforms/legacy/css/formreset.min.css?ver=2.8.17' type='text/css' media='all' /> <link rel='stylesheet' id='gforms_formsmain_css-css' href='https://www.thermofisher.com/blog/analyteguru/wp-content/plugins/gravityforms/legacy/css/formsmain.min.css?ver=2.8.17' type='text/css' media='all' /> <link rel='stylesheet' id='gforms_ready_class_css-css' href='https://www.thermofisher.com/blog/analyteguru/wp-content/plugins/gravityforms/legacy/css/readyclass.min.css?ver=2.8.17' type='text/css' media='all' /> <link rel='stylesheet' id='gforms_browsers_css-css' href='https://www.thermofisher.com/blog/analyteguru/wp-content/plugins/gravityforms/legacy/css/browsers.min.css?ver=2.8.17' type='text/css' media='all' /> <link rel='stylesheet' id='globals-css' href='https://www.thermofisher.com/blog/analyteguru/wp-content/themes/accelerating-science-multilingual-blogs/global-style.css?ver=1.2.9.3' type='text/css' media='all' /> <link rel='stylesheet' id='brightcove-pip-css-css' href='https://players.brightcove.net/videojs-pip/1/videojs-pip.css?ver=2.8.7' type='text/css' media='all' /> <link rel='stylesheet' id='brightcove-playlist-css' href='https://www.thermofisher.com/blog/analyteguru/wp-content/plugins/brightcove-video-connect/assets/css/brightcove_playlist.min.css?ver=2.8.7' type='text/css' media='all' /> <link rel='stylesheet' id='bfa-font-awesome-css' href='https://use.fontawesome.com/releases/v5.15.4/css/all.css?ver=2.0.3' type='text/css' media='all' /> <script type="text/javascript" src="https://www.thermofisher.com/blog/analyteguru/wp-content/plugins/google-analytics-premium/assets/js/frontend-gtag.min.js?ver=9.2.0" id="monsterinsights-frontend-script-js" async="async" data-wp-strategy="async"></script> <script data-cfasync="false" data-wpfc-render="false" type="text/javascript" id='monsterinsights-frontend-script-js-extra'>/* <![CDATA[ */ var monsterinsights_frontend = {"js_events_tracking":"true","download_extensions":"doc,pdf,ppt,zip,xls,docx,pptx,xlsx","inbound_paths":"[{\"path\":\"\\\/go\\\/\",\"label\":\"affiliate\"},{\"path\":\"\\\/recommend\\\/\",\"label\":\"affiliate\"}]","home_url":"https:\/\/admin.acceleratingscience.com\/analyteguru","hash_tracking":"false","v4_id":"G-TJ18KZWFVE"};/* ]]> */ </script> <script type="text/javascript" src="https://www.thermofisher.com/blog//wp-includes/js/jquery/jquery.min.js?ver=3.7.1" id="jquery-core-js"></script> <script type="text/javascript" src="https://www.thermofisher.com/blog//wp-includes/js/jquery/jquery-migrate.min.js?ver=3.4.1" id="jquery-migrate-js"></script> <script type="text/javascript" src="https://www.thermofisher.com/blog/analyteguru/wp-content/plugins/mashshare-google-analytics/assets/js/mashga.min.js?ver=1.0.9" id="mashga-js"></script> <script type="text/javascript" src="https://www.thermofisher.com/blog//wp-includes/js/underscore.min.js?ver=1.13.4" id="underscore-js"></script> <script type="text/javascript" src="https://www.thermofisher.com/blog//wp-includes/js/backbone.min.js?ver=1.5.0" id="backbone-js"></script> <script type="text/javascript" id="wp-api-js-js-extra"> /* <![CDATA[ */ var WP_API_Settings = {"root":"https:\/\/admin.acceleratingscience.com\/analyteguru","nonce":"29e0fbf17e"}; /* ]]> */ </script> <script type="text/javascript" src="https://www.thermofisher.com/blog/analyteguru/wp-content/plugins/wp-search-live/public/assets/js/util--wp-api.js?ver=0.9" id="wp-api-js-js"></script> <script type="text/javascript" defer='defer' src="https://www.thermofisher.com/blog/analyteguru/wp-content/plugins/gravityforms/js/jquery.json.min.js?ver=2.8.17" id="gform_json-js"></script> <script type="text/javascript" id="gform_gravityforms-js-extra"> /* <![CDATA[ */ var gf_global = {"gf_currency_config":{"name":"U.S. Dollar","symbol_left":"$","symbol_right":"","symbol_padding":"","thousand_separator":",","decimal_separator":".","decimals":2,"code":"USD"},"base_url":"https:\/\/admin.acceleratingscience.com\/analyteguru\/wp-content\/plugins\/gravityforms","number_formats":[],"spinnerUrl":"https:\/\/admin.acceleratingscience.com\/analyteguru\/wp-content\/plugins\/gravityforms\/images\/spinner.svg","version_hash":"4a792b070322e711c2521ee9802c04ea","strings":{"newRowAdded":"New row added.","rowRemoved":"Row removed","formSaved":"The form has been saved. The content contains the link to return and complete the form."}}; var gf_global = {"gf_currency_config":{"name":"U.S. Dollar","symbol_left":"$","symbol_right":"","symbol_padding":"","thousand_separator":",","decimal_separator":".","decimals":2,"code":"USD"},"base_url":"https:\/\/admin.acceleratingscience.com\/analyteguru\/wp-content\/plugins\/gravityforms","number_formats":[],"spinnerUrl":"https:\/\/admin.acceleratingscience.com\/analyteguru\/wp-content\/plugins\/gravityforms\/images\/spinner.svg","version_hash":"4a792b070322e711c2521ee9802c04ea","strings":{"newRowAdded":"New row added.","rowRemoved":"Row removed","formSaved":"The form has been saved. The content contains the link to return and complete the form."}}; var gform_i18n = {"datepicker":{"days":{"monday":"Mo","tuesday":"Tu","wednesday":"We","thursday":"Th","friday":"Fr","saturday":"Sa","sunday":"Su"},"months":{"january":"January","february":"February","march":"March","april":"April","may":"May","june":"June","july":"July","august":"August","september":"September","october":"October","november":"November","december":"December"},"firstDay":1,"iconText":"Select date"}}; var gf_legacy_multi = {"1":"1","2":"1"}; var gform_gravityforms = {"strings":{"invalid_file_extension":"This type of file is not allowed. Must be one of the following:","delete_file":"Delete this file","in_progress":"in progress","file_exceeds_limit":"File exceeds size limit","illegal_extension":"This type of file is not allowed.","max_reached":"Maximum number of files reached","unknown_error":"There was a problem while saving the file on the server","currently_uploading":"Please wait for the uploading to complete","cancel":"Cancel","cancel_upload":"Cancel this upload","cancelled":"Cancelled"},"vars":{"images_url":"https:\/\/admin.acceleratingscience.com\/analyteguru\/wp-content\/plugins\/gravityforms\/images"}}; /* ]]> */ </script> <script type="text/javascript" id="gform_gravityforms-js-before"> /* <![CDATA[ */ var gform;gform||(document.addEventListener("gform_main_scripts_loaded",function(){gform.scriptsLoaded=!0}),window.addEventListener("DOMContentLoaded",function(){gform.domLoaded=!0}),gform={domLoaded:!1,scriptsLoaded:!1,initializeOnLoaded:function(o){gform.domLoaded&&gform.scriptsLoaded?o():!gform.domLoaded&&gform.scriptsLoaded?window.addEventListener("DOMContentLoaded",o):document.addEventListener("gform_main_scripts_loaded",o)},hooks:{action:{},filter:{}},addAction:function(o,n,r,t){gform.addHook("action",o,n,r,t)},addFilter:function(o,n,r,t){gform.addHook("filter",o,n,r,t)},doAction:function(o){gform.doHook("action",o,arguments)},applyFilters:function(o){return gform.doHook("filter",o,arguments)},removeAction:function(o,n){gform.removeHook("action",o,n)},removeFilter:function(o,n,r){gform.removeHook("filter",o,n,r)},addHook:function(o,n,r,t,i){null==gform.hooks[o][n]&&(gform.hooks[o][n]=[]);var e=gform.hooks[o][n];null==i&&(i=n+"_"+e.length),gform.hooks[o][n].push({tag:i,callable:r,priority:t=null==t?10:t})},doHook:function(n,o,r){var t;if(r=Array.prototype.slice.call(r,1),null!=gform.hooks[n][o]&&((o=gform.hooks[n][o]).sort(function(o,n){return o.priority-n.priority}),o.forEach(function(o){"function"!=typeof(t=o.callable)&&(t=window[t]),"action"==n?t.apply(null,r):r[0]=t.apply(null,r)})),"filter"==n)return r[0]},removeHook:function(o,n,t,i){var r;null!=gform.hooks[o][n]&&(r=(r=gform.hooks[o][n]).filter(function(o,n,r){return!!(null!=i&&i!=o.tag||null!=t&&t!=o.priority)}),gform.hooks[o][n]=r)}}); /* ]]> */ </script> <script type="text/javascript" defer='defer' src="https://www.thermofisher.com/blog/analyteguru/wp-content/plugins/gravityforms/js/gravityforms.min.js?ver=2.8.17" id="gform_gravityforms-js"></script> <script type="text/javascript" defer='defer' src="https://www.thermofisher.com/blog/analyteguru/wp-content/plugins/gravityforms/js/chosen.jquery.min.js?ver=2.8.17" id="gform_chosen-js"></script> <script type="text/javascript" defer='defer' src="https://www.thermofisher.com/blog/analyteguru/wp-content/plugins/gravityforms/assets/js/dist/utils.min.js?ver=50c7bea9c2320e16728e44ae9fde5f26" id="gform_gravityforms_utils-js"></script> <script type="text/javascript" src="https://www.thermofisher.com/blog/analyteguru/wp-content/themes/accelerating-science-multilingual-blogs/js/single-scripts.js?ver=1.2.9.3" id="single-scripts-js"></script> <link rel="https://api.w.org/" href="https://www.thermofisher.com/blog/analyteguru/wp-json/" /><link rel="alternate" title="JSON" type="application/json" href="https://www.thermofisher.com/blog/analyteguru/wp-json/wp/v2/posts/3788" /><link rel="EditURI" type="application/rsd+xml" title="RSD" href="https://www.thermofisher.com/blog/xmlrpc.php?rsd" /> <link rel='shortlink' href='https://www.thermofisher.com/blog/analyteguru/?p=3788' /> <link rel="alternate" title="oEmbed (JSON)" type="application/json+oembed" href="https://www.thermofisher.com/blog/analyteguru/wp-json/oembed/1.0/embed?url=https%3A%2F%2Fwww.thermofisher.com%2Fblog%2Fanalyteguru%2Fimproving-agave-syrup-carbohydrate-analysis-with-automatic%2F" /> <link rel="alternate" title="oEmbed (XML)" type="text/xml+oembed" href="https://www.thermofisher.com/blog/analyteguru/wp-json/oembed/1.0/embed?url=https%3A%2F%2Fwww.thermofisher.com%2Fblog%2Fanalyteguru%2Fimproving-agave-syrup-carbohydrate-analysis-with-automatic%2F&format=xml" />  <link rel="icon" href="https://www.thermofisher.com/blog/analyteguru/wp-content/themes/accelerating-science-multilingual-blogs/images/favicon.ico" /> <script type="application/ld+json"> {"@context":"http:\/\/schema.org","@type":"BlogPosting","mainEntityofPage":{"@type":"WebPage","@id":"https:\/\/www.thermofisher.com\/blog\/analyteguru\/improving-agave-syrup-carbohydrate-analysis-with-automatic\/"},"headline":"Improving Agave Syrup Carbohydrate Analysis With Automatic Eluent Generation","datePublished":"10\/24\/2022","dateModified":"10\/24\/2022","publisher":{"@type":"Organization","name":"Thermo Fisher Scientific","URL":"https:\/\/www.thermofisher.com\/us\/en\/home.html","logo":{"@type":"ImageObject","url":"https:\/\/www.thermofisher.com\/content\/dam\/LifeTech\/Images\/Header\/logo-color.png"}},"image":{"@type":"ImageObject","url":"https:\/\/admin.acceleratingscience.com\/analyteguru\/wp-content\/uploads\/sites\/25\/2023\/06\/19075-og.jpg","width":998,"height":661},"keywords":"Food and Beverage, HPAE-PAD, ","author":{"@type":"Person","name":"AnalyteGuru Staff"}} </script> <script type="text/javascript" src="https://www.thermofisher.com/etc/designs/lifetech/clientlibs/digitaldata.js"></script> <script id="tagmanager" type="text/javascript" src='https://assets.adobedtm.com/launch-ENeecb77f1160a4928a492ff040f7110c7.min.js' defer="defer"></script> <script> (function($) { $(document).ready(function() { digitalData.setPageType('blog'); }) })(jQuery); </script> <script type="text/javascript" src="https://www.thermofisher.com/etc/designs/lifetech/clientlibs/digitaldata.js" defer="defer"></script> <script id="tagmanager" type="text/javascript" src='https://assets.adobedtm.com/launch-ENeecb77f1160a4928a492ff040f7110c7.min.js' defer="defer"></script> <script> (function($) { $(document).ready(function() { digitalData.setPageType('blog'); }) })(jQuery); </script> <style type="text/css" id="wp-custom-css"> .hero-banner { position: relative; } .hero-banner::before { content: ''; position: absolute; background: rgba(0,0,0,.3); top: 0; left: 0; right: 0; bottom: 0; } </style> </head> <body class="post-template-default single single-post postid-3788 single-format-standard header-image full-width-content genesis-breadcrumbs-hidden"><div class="site-container"><header class="site-header"><div class="wrap"><div class="title-area"><p class="site-title"><a href="https://www.thermofisher.com/" title="Thermo Fisher Scientific">Thermo Fisher Scientific</a></p><p class="site-description">Your educational resource for biopharma, pharma, environmental, food and agriculture, industrial, and clinical labs</p></div><div class="widget-area header-widget-area"><section id="nav_menu-2" class="widget widget_nav_menu"><div class="widget-wrap"><nav class="nav-header"><ul id="menu-main-menu" class="menu genesis-nav-menu"><li id="menu-item-13046" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-has-children menu-item-13046"><a><span >Categories</span></a> <ul class="sub-menu"> <li id="menu-item-13047" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-13047"><a href="https://www.thermofisher.com/blog/materials/"><span >Advancing Materials</span></a></li> <li id="menu-item-13048" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-13048"><a href="https://www.thermofisher.com/blog/mining/"><span >Advancing Mining</span></a></li> <li id="menu-item-13063" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-13063"><a href="https://www.thermofisher.com/blog/analyteguru/"><span >AnalyteGuru</span></a></li> <li id="menu-item-13049" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-13049"><a href="https://www.thermofisher.com/blog/metals/"><span >Analyzing Metals</span></a></li> <li id="menu-item-13050" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-13050"><a href="https://www.thermofisher.com/blog/ask-a-scientist/"><span >Ask a Scientist</span></a></li> <li id="menu-item-13051" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-13051"><a href="https://www.thermofisher.com/blog/behindthebench/"><span >Behind the Bench</span></a></li> <li id="menu-item-13052" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-13052"><a href="https://www.thermofisher.com/blog/biotechnology/"><span >Biotech at Scale</span></a></li> <li id="menu-item-13053" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-13053"><a href="https://www.thermofisher.com/blog/clinical-conversations/"><span >Clinical Conversations</span></a></li> <li id="menu-item-13054" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-13054"><a href="https://www.thermofisher.com/blog/food/"><span >Examining Food</span></a></li> <li id="menu-item-13055" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-13055"><a href="https://www.thermofisher.com/blog/identifying-threats/"><span >Identifying Threats</span></a></li> <li id="menu-item-13056" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-13056"><a href="https://www.thermofisher.com/blog/semiconductors/"><span >Illuminating Semiconductors</span></a></li> <li id="menu-item-13057" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-13057"><a href="https://www.thermofisher.com/blog/atomic-resolution/"><span >Life in Atomic Resolution</span></a></li> <li id="menu-item-13058" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-13058"><a href="https://www.thermofisher.com/blog/life-in-the-lab/"><span >Life in the Lab</span></a></li> <li id="menu-item-13059" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-13059"><a href="https://www.thermofisher.com/blog/oempowered/"><span >OEMpowered</span></a></li> <li id="menu-item-13060" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-13060"><a href="https://www.thermofisher.com/blog/connectedlab/"><span >The Connected Lab</span></a></li> </ul> </li> <li id="menu-item-13061" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-13061"><a href="https://www.thermofisher.com/blog/about-us/"><span >About Us</span></a></li> <li id="menu-item-13062" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-13062"><a href="https://www.thermofisher.com/blog/contact-us/?site=analyteguru"><span >Contact</span></a></li> </ul></nav></div></section> </div><div class="breadcrumb"><span class="breadcrumb-link-wrap"><a href="https://www.thermofisher.com/blog/?">Accelerating Science</a></span><span class="breadcrumb-link-wrap"><a class="breadcrumb-link" href="https://www.thermofisher.com/blog/analyteguru/"><span class="breadcrumb-link-text-wrap">AnalyteGuru</span></a><meta ></span> <span aria-label="breadcrumb separator">/</span> <span class="breadcrumb-link-wrap"><a class="breadcrumb-link" href="https://www.thermofisher.com/blog/analyteguru/hpae-pad/"><span class="breadcrumb-link-text-wrap">HPAE-PAD</span></a><meta ></span> <span aria-label="breadcrumb separator">/</span> <span class="breadcrumb-current-title">Improving Agave Syrup Carbohydrate Analysis With Automatic Eluent Generation</span></div></div></header><div class="site-inner"><div class="content-sidebar-wrap"><main class="content"><article class="post-3788 post type-post status-publish format-standard has-post-thumbnail category-food-beverage category-hpae-pad tag-food-and-beverage tag-hpae-pad entry" aria-label="Improving Agave Syrup Carbohydrate Analysis With Automatic Eluent Generation"><header class="entry-header"><h1 class="entry-title">Improving Agave Syrup Carbohydrate Analysis With Automatic Eluent Generation</h1> <span class="post-info-author"> By <a href="https://www.thermofisher.com/blog/analyteguru/tf-author/jingli-hu/" title="Posts by Jingli Hu" rel="author">Jingli Hu, Application Staff Scientist, Thermo Fisher Scientific</a> </span> <span class="post-info-date"> 10.24.2022 </span> </header><div class="entry-content"><div class="lia-message-body-content"> <p>Agave syrup is growing in popularity as an alternative to traditional sweeteners such as table sugar and honey. But as its use grows, so does the incentive for adulteration. High-performance anion chromatography with pulsed amperometric detection (HPAE-PAD) is recommended in the official guidelines for the characterization of agave syrup to determine product authenticity. Now, this can be performed in dual eluent generation cartridge (Dual EGC) mode for faster, more economical analysis.</p> <h3 id="toc-hId-1154435335"><strong>Agave syrup: A sweeter choice</strong></h3> <p><img decoding="async" class="alignright wp-image-3793 " src="https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2023/06/19075-og-300x199.jpg" alt="" width="403" height="267" srcset="https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2023/06/19075-og-300x199.jpg 300w, https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2023/06/19075-og-768x509.jpg 768w, https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2023/06/19075-og-96x65.jpg 96w, https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2023/06/19075-og.jpg 998w" sizes="(max-width: 403px) 100vw, 403px" />Over the past few years, agave syrup, produced from the sap of the agave plant in Mexico, has been widely adopted as a sweetener. The syrup boasts a low glycemic index (GI) (17-27) compared to honey (55) and sucrose (68),<a href="#_edn1" target="_blank" rel="noopener nofollow noreferrer" name="_ednref1">[i]</a> meaning that its carbohydrates are more slowly digested, absorbed, and metabolized. Agave nectar therefore causes a smaller rise in blood sugar and, consequently, insulin levels. In addition, it can provide the same level of sweetness as other glucose and sucrose-based syrups, but with fewer calories thanks to its high fructose content.</p> <p>The increased popularity of agave syrup means that inauthentic variations are entering circulation. As the syrup is mostly composed of carbohydrates, it is easy and cheap to adulterate using less expensive sweeteners, including high fructose corn syrup (HFCS). Knowing the composition of agave syrup using analytical techniques is therefore essential to ensuring authenticity and protecting human health through better consumer knowledge.</p> <h3 id="toc-hId-2041939016"><strong>Improving determination of sugars with Dual EGC</strong></h3> <p>The Mexican government has created official guidelines for characterizing agave syrup, known as Norma Oficial Mexicana (NOM)-003-SAGARPA-2016.<a href="#_edn2" target="_blank" rel="noopener nofollow noreferrer" name="_ednref2">[ii]</a> HPAE-PAD is the recommended NOM method for determination of sugars and detection of adulterants in agave syrup, as it enables direct detection of analytes — thereby eliminating errors that can be caused by analyte derivatization.</p> <p>However, Dual EGC mode can improve the recommended HPAE-PAD method by reducing eluent consumption and eliminating manual eluent preparation. The Dual EGC mode uses a methanesulfonic acid (MSA) EGC and potassium hydroxide (KOH) EGC sequentially to electrolytically produce a KOH/KMSA eluent. The eluent gradient is highly reproducible and accurate, and is perfect for separating complex carbohydrates such as those found in agave syrup.</p> <h3 id="toc-hId--1365524599"><strong>HPAE-PAD with Dual EGC: Linear, precise, sensitive and accurate</strong></h3> <p>In a recent application note, we performed <a href="https://www.thermofisher.com/document-connect/document-connect.html?url=https%3A%2F%2Fassets.thermofisher.com%2FTFS-Assets%2FCMD%2FApplication-Notes%2Fan-73896-ic-hpae-pad-carbohydrates-agave-syrup-cartridge-mode-an73896-en.pdf&title=QXBwbGljYXRpb24gTm90ZTogQ2FyYm9oeWRyYXRlIEFuYWx5c2lzIG9mIEFnYXZlIFN5cnVwIHVzaW5nIEhQQUUtUEFEIGluIER1YWwgRWx1ZW50IEdlbmVyYXRpb24gQ2FydHJpZGdlIE1vZGU=" target="_blank" rel="nofollow noopener">carbohydrate analysis of agave syrup</a> with HPAE-PAD using a Thermo Scientific™ <a href="https://www.thermofisher.com/order/catalog/product/062896" target="_blank" rel="nofollow noopener">Dionex™ CarboPac™ PA200</a>-1 mm column (250 × 1 mm) in Dual EGC mode. We analyzed three different agave syrup samples, and determined the main sugars (fructose, glucose, and sucrose), polyols (sorbitol and mannitol), and 5-hydroxymethylfurfural (HMF). We also evaluated the presence of adulterants (HFCS or corn syrup) using HPAE-PAD and amyloglucosidase enzymatic hydrolysis.</p> <p>The Dionex CarboPac PA200-1 mm column is the recommended column for separating charged and neutral oligosaccharides, as it can provide high-resolution separation.<a href="#_edn3" target="_blank" rel="noopener nofollow noreferrer" name="_ednref3">[iii]</a> When tested on agave syrup, we found excellent resolution (>2.0) between all the components (Figure 1).</p> <p><span class="lia-inline-image-display-wrapper lia-image-align-center" style="width: 539px"><span class="lia-inline-image-caption"><img decoding="async" class="aligncenter size-full wp-image-13177" src="https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2023/06/102422-Figure-1-Separation-of-agave-sugar-1.png" alt="" width="539" height="304" srcset="https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2023/06/102422-Figure-1-Separation-of-agave-sugar-1.png 539w, https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2023/06/102422-Figure-1-Separation-of-agave-sugar-1-300x169.png 300w" sizes="(max-width: 539px) 100vw, 539px" />Figure 1: Separation of seven agave sugar standards using a Dionex CarboPac PA200-1 mm column set.</span></span></p> <p>We investigated the key parameters of the HPAE-PAD method with Dual EGC mode for carbohydrate analysis of agave syrup, and found the method was:</p> <ul> <li><strong>Linear: </strong>A coefficient of determination of <0.999> was obtained for each sugar.</li> <li><strong>Precise:</strong> Calculated peak area precision was 0.85 – 1.66%, with retention time precision <0.28% (Table 1).</li> <li><strong>Sensitive:</strong> The limit of detection (LOD) and limit of quantitation (LOQ) for all seven sugars were below 11.56 μg/L and 48.6 μg/L, respectively.</li> <li><strong>Accurate:</strong> Recovery of sorbitol, glucose, and sucrose from spiked samples was 97.2 – 105%.</li> </ul> <p><span class="lia-inline-image-display-wrapper lia-image-align-center" style="width: 743px"><span class="lia-inline-image-caption"><img loading="lazy" decoding="async" class="aligncenter size-full wp-image-13179" src="https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2023/06/102422-Table-1-Retention-1.png" alt="" width="743" height="88" srcset="https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2023/06/102422-Table-1-Retention-1.png 743w, https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2023/06/102422-Table-1-Retention-1-300x36.png 300w" sizes="(max-width: 743px) 100vw, 743px" />Table 1: Retention time and peak area precision. RSD: relative standard deviation.</span></span></p> <p>Importantly, the results were comparable to those obtained using manually prepared sodium hydroxide and sodium acetate eluent in NOM, but with superior precision.<sup><a href="#_edn3" target="_blank" rel="noopener nofollow noreferrer" name="_ednref3">[iii]</a></sup></p> <h3 id="toc-hId--478020918"><strong>Fighting adulterated agave syrup with Dual EGC</strong></h3> <p>Overall, HPAE-PAD in Dual EGC mode is an excellent tool for agave syrup carbohydrate analysis, with excellent linearity, precision, sensitivity, and accuracy. The method delivers resolution of agave carbohydrates similar to that achieved with standard HPAE-PAD, but with improved retention time precision. The quality of results and simplified operation makes the method a valuable tool in agave syrup adulteration detection.</p> <p>For more details on <a href="https://www.thermofisher.com/document-connect/document-connect.html?url=https%3A%2F%2Fassets.thermofisher.com%2FTFS-Assets%2FCMD%2FApplication-Notes%2Fan-73896-ic-hpae-pad-carbohydrates-agave-syrup-cartridge-mode-an73896-en.pdf&title=QXBwbGljYXRpb24gTm90ZTogQ2FyYm9oeWRyYXRlIEFuYWx5c2lzIG9mIEFnYXZlIFN5cnVwIHVzaW5nIEhQQUUtUEFEIGluIER1YWwgRWx1ZW50IEdlbmVyYXRpb24gQ2FydHJpZGdlIE1vZGU=" target="_blank" rel="nofollow noopener">improving carbohydrate analysis of agave syrup using HPAE-PAD</a>, read our application note, and keep an eye out for our next blog to learn how ion chromatography can be used for determination of nitrite and nitrate in sugar.</p> <p><a href="#_ednref1" target="_blank" rel="noopener nofollow noreferrer" name="_edn1">[i]</a> a) Wolever, T.; Vuksan, V.; Jenkins, A. L.; Campbell, J. Determination of glycemic index of: Organic 100% blue agave syrup and prebiotic 100% blue agave syrup; Final report for Glycemic Index Laboratories: Toronto, ON, November 2011. b) Foster-Powell, K.; Holt, S.H.A.; Brand-Miller, J.C. International table of glycemic index and glycemic load values: Am. J. Clin Nutr. 2002, 76, 5–56.</p> <p><a href="#_ednref2" target="_blank" rel="noopener nofollow noreferrer" name="_edn2">[ii]</a> NORMA Oficial Mexicana NOM-003-SAGARPA-2016.</p> <p><a href="#_ednref3" target="_blank" rel="noopener nofollow noreferrer" name="_edn3">[iii]</a> Thermo Fisher Scientific Application Note 73896: <a href="https://assets.thermofisher.com/TFS-Assets/CMD/Application-Notes/an-73896-ic-hpae-pad-carbohydrates-agave-syrup-cartridge-mode-an73896-en.pdf" target="_blank" rel="noopener">Carbohydrate analysis of agave syrup using HPAE-PAD in dual eluent generation cartridge mode.</a></p> <p><span style="color: #ff0000"><em><strong>Dr. Jeffrey Rohrer, Director of Applications Development, contributed to this article.</strong></em></span></p> </div> </div></article><section class="author-box"><h4 class="author-box-title">Jingli Hu</h4><div class="author-box-content" itemprop="description"></div></section><div class="adjacent-entry-pagination pagination"><div class="pagination-previous alignleft"><i class="fa fa-icon fa-chevron-left"></i> <a href="https://www.thermofisher.com/blog/analyteguru/why-zombies-make-bad-chromatographers/" rel="prev">Why Zombies Make BAD Chromatographers</a></div><div class="pagination-next alignright"><a href="https://www.thermofisher.com/blog/analyteguru/configure-your-next-gc-gc-ms-system-online-in-minutes/" rel="next">Configure Your Next GC-GC-MS System Online in Minutes</a> <i class="fa fa-icon fa-chevron-right"></i></div></div></main></div></div><footer class="site-footer"><div class="wrap"><div class="creds"><p class="footer-links"><a href="https://corporate.thermofisher.com/en/footer/privacy-statement.html" target="_blank" rel="noopener noreferrer">Privacy Statement</a><a href="https://corporate.thermofisher.com/en/footer/terms-and-conditions.html" target="_blank" rel="noopener noreferrer">Terms & Conditions</a><a href="/blog/locations/">Locations</a><a href="/blog/sitemap/">Sitemap</a></p><p>© 2024 <a href="https://www.thermofisher.com">Thermo Fisher Scientific</a>. All Rights Reserved.</p></div><p></p><span class="social-links"><p>Talk to us</p><a href="https://www.facebook.com/ChromatographySolutions" class="facebook" target="_blank" rel="noopener noreferrer"><i class="fa fa-icon fa-facebook"></i></a><a href="https://www.linkedin.com/showcase/chromatography-&-mass-spectrometry/" class="linkedin" target="_blank" rel="noopener noreferrer"><i class="fa fa-icon fa-linkedin"></i></a><a href="https://twitter.com/ChromSolutions" class="twitter" target="_blank" rel="noopener noreferrer"><i class="fa fa-icon fa-twitter"></i></a><a href="https://www.youtube.com/c/thermoscientific" class="youtube" target="_blank" rel="noopener noreferrer"><i class="fa fa-icon fa-youtube"></i></a></span></div></footer></div>  <script type="text/html" id="wpls--tmpl"> <li class="search-result"> <a href="<%= post.link %>"> <div class="wpls--item-title-wrap"> <h4 class="wpls--item-title"><%= post.title %></h4> </div> </a> </li> </script> <script type="text/javascript"> /* MonsterInsights Scroll Tracking */ if ( typeof(jQuery) !== 'undefined' ) { jQuery( document ).ready(function(){ function monsterinsights_scroll_tracking_load() { if ( ( typeof(__gaTracker) !== 'undefined' && __gaTracker && __gaTracker.hasOwnProperty( "loaded" ) && __gaTracker.loaded == true ) || ( typeof(__gtagTracker) !== 'undefined' && __gtagTracker ) ) { (function(factory) { factory(jQuery); }(function($) { /* Scroll Depth */ "use strict"; var defaults = { percentage: true }; var $window = $(window), cache = [], scrollEventBound = false, lastPixelDepth = 0; /* * Plugin */ $.scrollDepth = function(options) { var startTime = +new Date(); options = $.extend({}, defaults, options); /* * Functions */ function sendEvent(action, label, scrollDistance, timing) { if ( 'undefined' === typeof MonsterInsightsObject || 'undefined' === typeof MonsterInsightsObject.sendEvent ) { return; } var paramName = action.toLowerCase(); var fieldsArray = { send_to: 'G-TJ18KZWFVE', non_interaction: true }; fieldsArray[paramName] = label; if (arguments.length > 3) { fieldsArray.scroll_timing = timing MonsterInsightsObject.sendEvent('event', 'scroll_depth', fieldsArray); } else { MonsterInsightsObject.sendEvent('event', 'scroll_depth', fieldsArray); } } function calculateMarks(docHeight) { return { '25%' : parseInt(docHeight * 0.25, 10), '50%' : parseInt(docHeight * 0.50, 10), '75%' : parseInt(docHeight * 0.75, 10), /* Cushion to trigger 100% event in iOS */ '100%': docHeight - 5 }; } function checkMarks(marks, scrollDistance, timing) { /* Check each active mark */ $.each(marks, function(key, val) { if ( $.inArray(key, cache) === -1 && scrollDistance >= val ) { sendEvent('Percentage', key, scrollDistance, timing); cache.push(key); } }); } function rounded(scrollDistance) { /* Returns String */ return (Math.floor(scrollDistance/250) * 250).toString(); } function init() { bindScrollDepth(); } /* * Public Methods */ /* Reset Scroll Depth with the originally initialized options */ $.scrollDepth.reset = function() { cache = []; lastPixelDepth = 0; $window.off('scroll.scrollDepth'); bindScrollDepth(); }; /* Add DOM elements to be tracked */ $.scrollDepth.addElements = function(elems) { if (typeof elems == "undefined" || !$.isArray(elems)) { return; } $.merge(options.elements, elems); /* If scroll event has been unbound from window, rebind */ if (!scrollEventBound) { bindScrollDepth(); } }; /* Remove DOM elements currently tracked */ $.scrollDepth.removeElements = function(elems) { if (typeof elems == "undefined" || !$.isArray(elems)) { return; } $.each(elems, function(index, elem) { var inElementsArray = $.inArray(elem, options.elements); var inCacheArray = $.inArray(elem, cache); if (inElementsArray != -1) { options.elements.splice(inElementsArray, 1); } if (inCacheArray != -1) { cache.splice(inCacheArray, 1); } }); }; /* * Throttle function borrowed from: * Underscore.js 1.5.2 * http://underscorejs.org * (c) 2009-2013 Jeremy Ashkenas, DocumentCloud and Investigative Reporters & Editors * Underscore may be freely distributed under the MIT license. */ function throttle(func, wait) { var context, args, result; var timeout = null; var previous = 0; var later = function() { previous = new Date; timeout = null; result = func.apply(context, args); }; return function() { var now = new Date; if (!previous) previous = now; var remaining = wait - (now - previous); context = this; args = arguments; if (remaining <= 0) { clearTimeout(timeout); timeout = null; previous = now; result = func.apply(context, args); } else if (!timeout) { timeout = setTimeout(later, remaining); } return result; }; } /* * Scroll Event */ function bindScrollDepth() { scrollEventBound = true; $window.on('scroll.scrollDepth', throttle(function() { /* * We calculate document and window height on each scroll event to * account for dynamic DOM changes. */ var docHeight = $(document).height(), winHeight = window.innerHeight ? window.innerHeight : $window.height(), scrollDistance = $window.scrollTop() + winHeight, /* Recalculate percentage marks */ marks = calculateMarks(docHeight), /* Timing */ timing = +new Date - startTime; checkMarks(marks, scrollDistance, timing); }, 500)); } init(); }; /* UMD export */ return $.scrollDepth; })); jQuery.scrollDepth(); } else { setTimeout(monsterinsights_scroll_tracking_load, 200); } } monsterinsights_scroll_tracking_load(); }); } /* End MonsterInsights Scroll Tracking */ </script><script type="text/javascript" id="wpls-script-js-extra"> /* <![CDATA[ */ var wp_search_vars = {"helperText":"one more character","api":"https:\/\/admin.acceleratingscience.com\/wp-json\/as\/v1\/all-posts\/25"}; /* ]]> */ </script> <script type="text/javascript" src="https://www.thermofisher.com/blog/analyteguru/wp-content/plugins/wp-search-live/public/assets/js/wp-live-search.js?ver=0.9" id="wpls-script-js"></script> <script type="text/javascript" src="https://www.thermofisher.com/blog//wp-includes/js/comment-reply.min.js?ver=6.6.1" id="comment-reply-js" async="async" data-wp-strategy="async"></script> <script type="text/javascript" src="https://www.thermofisher.com/blog//wp-includes/js/dist/dom-ready.min.js?ver=f77871ff7694fffea381" id="wp-dom-ready-js"></script> <script type="text/javascript" src="https://www.thermofisher.com/blog//wp-includes/js/dist/hooks.min.js?ver=2810c76e705dd1a53b18" id="wp-hooks-js"></script> <script type="text/javascript" src="https://www.thermofisher.com/blog//wp-includes/js/dist/i18n.min.js?ver=5e580eb46a90c2b997e6" id="wp-i18n-js"></script> <script type="text/javascript" id="wp-i18n-js-after"> /* <![CDATA[ */ wp.i18n.setLocaleData( { 'text direction\u0004ltr': [ 'ltr' ] } ); /* ]]> */ </script> <script type="text/javascript" src="https://www.thermofisher.com/blog//wp-includes/js/dist/a11y.min.js?ver=d90eebea464f6c09bfd5" id="wp-a11y-js"></script> <script type="text/javascript" defer='defer' src="https://www.thermofisher.com/blog/analyteguru/wp-content/plugins/gravityforms/assets/js/dist/vendor-theme.min.js?ver=54e7080aa7a02c83aa61fae430b9d869" id="gform_gravityforms_theme_vendors-js"></script> <script type="text/javascript" id="gform_gravityforms_theme-js-extra"> /* <![CDATA[ */ var gform_theme_config = {"common":{"form":{"honeypot":{"version_hash":"4a792b070322e711c2521ee9802c04ea"}}},"hmr_dev":"","public_path":"https:\/\/admin.acceleratingscience.com\/analyteguru\/wp-content\/plugins\/gravityforms\/assets\/js\/dist\/"}; /* ]]> */ </script> <script type="text/javascript" defer='defer' src="https://www.thermofisher.com/blog/analyteguru/wp-content/plugins/gravityforms/assets/js/dist/scripts-theme.min.js?ver=bab19fd84843dabc070e73326d787910" id="gform_gravityforms_theme-js"></script> <script type="text/javascript" defer='defer' src="https://www.thermofisher.com/blog/analyteguru/wp-content/plugins/gravityforms/js/placeholders.jquery.min.js?ver=2.8.17" id="gform_placeholder-js"></script> <script type="text/javascript" src="https://www.thermofisher.com/blog//wp-includes/js/jquery/ui/core.min.js?ver=1.13.3" id="jquery-ui-core-js"></script> <script type="text/javascript" src="https://www.thermofisher.com/blog//wp-includes/js/jquery/ui/tabs.min.js?ver=1.13.3" id="jquery-ui-tabs-js"></script> <script type="text/javascript" src="https://www.thermofisher.com/blog/analyteguru/wp-content/themes/accelerating-science-multilingual-blogs/js/scripts.js?ver=1.2.9.3" id="scripts-js"></script> <script type="text/javascript" src="/K1tG87Y8lf3h/SMnlrP/_GYOkD/LOVucQL4wVLzt1/YxxmKQ/CQB/oTCJAXxg"></script></body></html>