CINXE.COM

individual fluorescent molecules

<!DOCTYPE html> <html lang="en" dir="ltr" prefix="content: http://purl.org/rss/1.0/modules/content/ dc: http://purl.org/dc/terms/ foaf: http://xmlns.com/foaf/0.1/ og: http://ogp.me/ns# rdfs: http://www.w3.org/2000/01/rdf-schema# schema: http://schema.org/ sioc: http://rdfs.org/sioc/ns# sioct: http://rdfs.org/sioc/types# skos: http://www.w3.org/2004/02/skos/core# xsd: http://www.w3.org/2001/XMLSchema# "> <head><script type="text/javascript" src="/_static/js/bundle-playback.js?v=HxkREWBo" charset="utf-8"></script> <script type="text/javascript" src="/_static/js/wombat.js?v=txqj7nKC" charset="utf-8"></script> <script>window.RufflePlayer=window.RufflePlayer||{};window.RufflePlayer.config={"autoplay":"on","unmuteOverlay":"hidden"};</script> <script type="text/javascript" src="/_static/js/ruffle/ruffle.js"></script> <script type="text/javascript"> __wm.init("https://web.archive.org/web"); __wm.wombat("https://www.nist.gov/image/moleculespng","20230208102344","https://web.archive.org/","web","/_static/", "1675851824"); </script> <link rel="stylesheet" type="text/css" href="/_static/css/banner-styles.css?v=S1zqJCYt" /> <link rel="stylesheet" type="text/css" href="/_static/css/iconochive.css?v=3PDvdIFv" /> <!-- End Wayback Rewrite JS Include --> <meta charset="utf-8"/><script type="text/javascript">(window.NREUM||(NREUM={})).init={ajax:{deny_list:["gov-bam.nr-data.net"]}};(window.NREUM||(NREUM={})).loader_config={licenseKey:"37b7ccb661",applicationID:"1089704227"};;(()=>{var e,t,r={9071:(e,t,r)=>{"use strict";r.d(t,{I:()=>n});var n=0,i=navigator.userAgent.match(/Firefox[\/\s](\d+\.\d+)/);i&&(n=+i[1])},6900:(e,t,r)=>{"use strict";let n;if(r.d(t,{H:()=>i}),r(2374).il){const e=document.createElement("div");e.innerHTML="\x3c!--[if lte IE 6]><div></div><![endif]--\x3e\x3c!--[if lte IE 7]><div></div><![endif]--\x3e\x3c!--[if lte IE 8]><div></div><![endif]--\x3e\x3c!--[if lte IE 9]><div></div><![endif]--\x3e",n=e.getElementsByTagName("div").length}var i;i=4===n?6:3===n?7:2===n?8:1===n?9:0},6562:(e,t,r)=>{"use strict";r.d(t,{P_:()=>h,Mt:()=>v,C5:()=>d,DL:()=>w,OP:()=>B,lF:()=>V,Yu:()=>A,Dg:()=>p,CX:()=>l,GE:()=>y,sU:()=>M});var n={};r.r(n),r.d(n,{agent:()=>O,match:()=>_,version:()=>S});var i=r(6797),o=r(909),a=r(8610);class s{constructor(e,t){try{if(!e||"object"!=typeof e)return(0,a.Z)("New setting a Configurable requires an object as input");if(!t||"object"!=typeof t)return(0,a.Z)("Setting a Configurable requires a model to set its initial properties");Object.assign(this,t),Object.entries(e).forEach((e=>{let[t,r]=e;const n=(0,o.q)(t);n.length&&r&&"object"==typeof r&&n.forEach((e=>{e in r&&((0,a.Z)(`"${e}" is a protected attribute and can not be changed in feature ${t}. It will have no effect.`),delete r[e])})),this[t]=r}))}catch(e){(0,a.Z)("An error occured while setting a Configurable",e)}}}const c={beacon:i.ce.beacon,errorBeacon:i.ce.errorBeacon,licenseKey:void 0,applicationID:void 0,sa:void 0,queueTime:void 0,applicationTime:void 0,ttGuid:void 0,user:void 0,account:void 0,product:void 0,extra:void 0,jsAttributes:{},userAttributes:void 0,atts:void 0,transactionName:void 0,tNamePlain:void 0},u={};function d(e){if(!e)throw new Error("All info objects require an agent identifier!");if(!u[e])throw new Error(`Info for ${e} was never set`);return u[e]}function l(e,t){if(!e)throw new Error("All info objects require an agent identifier!");u[e]=new s(t,c),(0,i.Qy)(e,u[e],"info")}const f={allow_bfcache:!1,privacy:{cookies_enabled:!0},ajax:{deny_list:void 0,enabled:!0,harvestTimeSeconds:10},distributed_tracing:{enabled:void 0,exclude_newrelic_header:void 0,cors_use_newrelic_header:void 0,cors_use_tracecontext_headers:void 0,allowed_origins:void 0},ssl:void 0,obfuscate:void 0,jserrors:{enabled:!0,harvestTimeSeconds:10},metrics:{enabled:!0,harvestTimeSeconds:10},page_action:{enabled:!0,harvestTimeSeconds:30},page_view_event:{enabled:!0},page_view_timing:{enabled:!0,harvestTimeSeconds:30},session_trace:{enabled:!0,harvestTimeSeconds:10},spa:{enabled:!0,harvestTimeSeconds:10}},g={};function h(e){if(!e)throw new Error("All configuration objects require an agent identifier!");if(!g[e])throw new Error(`Configuration for ${e} was never set`);return g[e]}function p(e,t){if(!e)throw new Error("All configuration objects require an agent identifier!");g[e]=new s(t,f),(0,i.Qy)(e,g[e],"config")}function v(e,t){if(!e)throw new Error("All configuration objects require an agent identifier!");var r=h(e);if(r){for(var n=t.split("."),i=0;i<n.length-1;i++)if("object"!=typeof(r=r[n[i]]))return;r=r[n[n.length-1]]}return r}const m={accountID:void 0,trustKey:void 0,agentID:void 0,licenseKey:void 0,applicationID:void 0,xpid:void 0},b={};function w(e){if(!e)throw new Error("All loader-config objects require an agent identifier!");if(!b[e])throw new Error(`LoaderConfig for ${e} was never set`);return b[e]}function y(e,t){if(!e)throw new Error("All loader-config objects require an agent identifier!");b[e]=new s(t,m),(0,i.Qy)(e,b[e],"loader_config")}const A=(0,i.mF)().o;var x=r(6900),E=r(2053),O=null,S=null;if(navigator.userAgent){var D=navigator.userAgent,j=D.match(/Version\/(\S+)\s+Safari/);j&&-1===D.indexOf("Chrome")&&-1===D.indexOf("Chromium")&&(O="Safari",S=j[1])}function _(e,t){if(!O)return!1;if(e!==O)return!1;if(!t)return!0;if(!S)return!1;for(var r=S.split("."),n=t.split("."),i=0;i<n.length;i++)if(n[i]!==r[i])return!1;return!0}var k=r(5526),T=r(2374);const P="NRBA_SESSION_ID";function C(){if(!T.il)return null;try{let e;return null===(e=window.sessionStorage.getItem(P))&&(e=(0,k.ky)(16),window.sessionStorage.setItem(P,e)),e}catch(e){return null}}var I=T._A?.XMLHttpRequest,N=I&&I.prototype;const R={};function B(e){if(!e)throw new Error("All runtime objects require an agent identifier!");if(!R[e])throw new Error(`Runtime for ${e} was never set`);return R[e]}function M(e,t){if(!e)throw new Error("All runtime objects require an agent identifier!");var r;R[e]=new s(t,(r=e,{customTransaction:void 0,disabled:!1,features:{},isolatedBacklog:!1,loaderType:void 0,maxBytes:6===x.H?2e3:3e4,offset:(0,E.yf)(),onerror:void 0,origin:""+T._A?.location,ptid:void 0,releaseIds:{},sessionId:1==v(r,"privacy.cookies_enabled")?C():null,xhrWrappable:I&&N&&N.addEventListener&&!/CriOS/.test(navigator.userAgent),userAgent:n})),(0,i.Qy)(e,R[e],"runtime")}function V(e){return function(e){try{const t=d(e);return!!t.licenseKey&&!!t.errorBeacon&&!!t.applicationID}catch(e){return!1}}(e)}},8226:(e,t,r)=>{"use strict";r.d(t,{q:()=>n});const n="1223.PROD"},9557:(e,t,r)=>{"use strict";r.d(t,{w:()=>o});var n=r(8610);const i={agentIdentifier:""};class o{constructor(e){try{if("object"!=typeof e)return(0,n.Z)("shared context requires an object as input");this.sharedContext={},Object.assign(this.sharedContext,i),Object.entries(e).forEach((e=>{let[t,r]=e;Object.keys(i).includes(t)&&(this.sharedContext[t]=r)}))}catch(e){(0,n.Z)("An error occured while setting SharedContext",e)}}}},4329:(e,t,r)=>{"use strict";r.d(t,{L:()=>d,R:()=>c});var n=r(3752),i=r(7022),o=r(4045),a=r(2325);const s={};function c(e,t){const r={staged:!1,priority:a.p[t]||0};u(e),s[e].get(t)||s[e].set(t,r)}function u(e){e&&(s[e]||(s[e]=new Map))}function d(){let e=arguments.length>0&&void 0!==arguments[0]?arguments[0]:"",t=arguments.length>1&&void 0!==arguments[1]?arguments[1]:"feature";if(u(e),!e||!s[e].get(t))return a(t);s[e].get(t).staged=!0;const r=Array.from(s[e]);function a(t){const r=e?n.ee.get(e):n.ee,a=o.X.handlers;if(r.backlog&&a){var s=r.backlog[t],c=a[t];if(c){for(var u=0;s&&u<s.length;++u)l(s[u],c);(0,i.D)(c,(function(e,t){(0,i.D)(t,(function(t,r){r[0].on(e,r[1])}))}))}delete a[t],r.backlog[t]=null,r.emit("drain-"+t,[])}}r.every((e=>{let[t,r]=e;return r.staged}))&&(r.sort(((e,t)=>e[1].priority-t[1].priority)),r.forEach((e=>{let[t]=e;a(t)})))}function l(e,t){var r=e[1];(0,i.D)(t[r],(function(t,r){var n=e[0];if(r[0]===n){var i=r[1],o=e[3],a=e[2];i.apply(o,a)}}))}},3752:(e,t,r)=>{"use strict";r.d(t,{ee:()=>u});var n=r(6797),i=r(3916),o=r(7022),a=r(6562),s="nr@context";let c=(0,n.fP)();var u;function d(){}function l(){return new d}function f(){(u.backlog.api||u.backlog.feature)&&(u.aborted=!0,u.backlog={})}c.ee?u=c.ee:(u=function e(t,r){var n={},c={},g={},h=!1;try{h=16===r.length&&(0,a.OP)(r).isolatedBacklog}catch(e){}var p={on:b,addEventListener:b,removeEventListener:w,emit:m,get:A,listeners:y,context:v,buffer:x,abort:f,aborted:!1,isBuffering:E,debugId:r,backlog:h?{}:t&&"object"==typeof t.backlog?t.backlog:{}};return p;function v(e){return e&&e instanceof d?e:e?(0,i.X)(e,s,l):l()}function m(e,r,n,i,o){if(!1!==o&&(o=!0),!u.aborted||i){t&&o&&t.emit(e,r,n);for(var a=v(n),s=y(e),d=s.length,l=0;l<d;l++)s[l].apply(a,r);var f=O()[c[e]];return f&&f.push([p,e,r,a]),a}}function b(e,t){n[e]=y(e).concat(t)}function w(e,t){var r=n[e];if(r)for(var i=0;i<r.length;i++)r[i]===t&&r.splice(i,1)}function y(e){return n[e]||[]}function A(t){return g[t]=g[t]||e(p,t)}function x(e,t){var r=O();p.aborted||(0,o.D)(e,(function(e,n){t=t||"feature",c[n]=t,t in r||(r[t]=[])}))}function E(e){return!!O()[c[e]]}function O(){return p.backlog}}(void 0,"globalEE"),c.ee=u)},9252:(e,t,r)=>{"use strict";r.d(t,{E:()=>n,p:()=>i});var n=r(3752).ee.get("handle");function i(e,t,r,i,o){o?(o.buffer([e],i),o.emit(e,t,r)):(n.buffer([e],i),n.emit(e,t,r))}},4045:(e,t,r)=>{"use strict";r.d(t,{X:()=>o});var n=r(9252);o.on=a;var i=o.handlers={};function o(e,t,r,o){a(o||n.E,i,e,t,r)}function a(e,t,r,i,o){o||(o="feature"),e||(e=n.E);var a=t[o]=t[o]||{};(a[r]=a[r]||[]).push([e,i])}},8544:(e,t,r)=>{"use strict";r.d(t,{bP:()=>s,iz:()=>c,m$:()=>a});var n=r(2374),i=!1;try{var o=Object.defineProperty({},"passive",{get:function(){i=!0}});n._A?.addEventListener("testPassive",null,o),n._A?.removeEventListener("testPassive",null,o)}catch(e){}function a(e){return i?{passive:!0,capture:!!e}:!!e}function s(e,t){let r=arguments.length>2&&void 0!==arguments[2]&&arguments[2];window.addEventListener(e,t,a(r))}function c(e,t){let r=arguments.length>2&&void 0!==arguments[2]&&arguments[2];document.addEventListener(e,t,a(r))}},5526:(e,t,r)=>{"use strict";r.d(t,{Rl:()=>i,ky:()=>o});var n=r(2374);function i(){var e=null,t=0,r=n._A?.crypto||n._A?.msCrypto;function i(){return e?15&e[t++]:16*Math.random()|0}r&&r.getRandomValues&&(e=r.getRandomValues(new Uint8Array(31)));for(var o,a="xxxxxxxx-xxxx-4xxx-yxxx-xxxxxxxxxxxx",s="",c=0;c<a.length;c++)s+="x"===(o=a[c])?i().toString(16):"y"===o?(o=3&i()|8).toString(16):o;return s}function o(e){var t=null,r=0,n=self.crypto||self.msCrypto;n&&n.getRandomValues&&Uint8Array&&(t=n.getRandomValues(new Uint8Array(31)));for(var i=[],o=0;o<e;o++)i.push(a().toString(16));return i.join("");function a(){return t?15&t[r++]:16*Math.random()|0}}},2053:(e,t,r)=>{"use strict";r.d(t,{nb:()=>c,os:()=>u,yf:()=>s,zO:()=>a});var n=r(7145),i=(new Date).getTime(),o=i;function a(){return n.G&&performance.now?Math.round(performance.now()):(i=Math.max((new Date).getTime(),i))-o}function s(){return i}function c(e){o=e}function u(){return o}},7145:(e,t,r)=>{"use strict";r.d(t,{G:()=>n});const n=void 0!==r(2374)._A?.performance?.timing?.navigationStart},6625:(e,t,r)=>{"use strict";r.d(t,{s:()=>c,v:()=>u});var n=r(8283),i=r(9071),o=r(2053),a=r(7145),s=r(2374);let c=!0;function u(e){var t=function(){if(i.I&&i.I<9)return;if(a.G)return c=!1,s._A?.performance?.timing?.navigationStart}();t&&((0,n.B)(e,"starttime",t),(0,o.nb)(t))}},8283:(e,t,r)=>{"use strict";r.d(t,{B:()=>o,L:()=>a});var n=r(2053),i={};function o(e,t,r){void 0===r&&(r=(0,n.zO)()+(0,n.os)()),i[e]=i[e]||{},i[e][t]=r}function a(e,t,r,n){const o=e.sharedContext.agentIdentifier;var a=i[o]?.[r],s=i[o]?.[n];void 0!==a&&void 0!==s&&e.store("measures",t,{value:s-a})}},9548:(e,t,r)=>{"use strict";r.d(t,{T:()=>i});var n=r(2374);const i={isFileProtocol:function(){let e=Boolean("file:"===(0,n.lW)()?.location?.protocol);e&&(i.supportabilityMetricSent=!0);return e},supportabilityMetricSent:!1}},8610:(e,t,r)=>{"use strict";function n(e,t){console&&console.warn&&"function"==typeof console.warn&&(console.warn(`New Relic: ${e}`),t&&console.warn(t))}r.d(t,{Z:()=>n})},3916:(e,t,r)=>{"use strict";r.d(t,{X:()=>i});var n=Object.prototype.hasOwnProperty;function i(e,t,r){if(n.call(e,t))return e[t];var i=r();if(Object.defineProperty&&Object.keys)try{return Object.defineProperty(e,t,{value:i,writable:!0,enumerable:!1}),i}catch(e){}return e[t]=i,i}},2374:(e,t,r)=>{"use strict";r.d(t,{_A:()=>o,il:()=>n,lW:()=>a,v6:()=>i});const n=Boolean("undefined"!=typeof window&&window.document),i=Boolean("undefined"!=typeof WorkerGlobalScope&&self.navigator instanceof WorkerNavigator);let o=(()=>{if(n)return window;if(i){if("undefined"!=typeof globalThis&&globalThis instanceof WorkerGlobalScope)return globalThis;if(self instanceof WorkerGlobalScope)return self}throw new Error("New Relic browser agent shutting down due to error: Unable to locate global scope. This is possibly due to code redefining browser global variables like `self` and `window`.")})();function a(){return o}},7022:(e,t,r)=>{"use strict";r.d(t,{D:()=>i});var n=Object.prototype.hasOwnProperty;function i(e,t){var r=[],i="",o=0;for(i in e)n.call(e,i)&&(r[o]=t(i,e[i]),o+=1);return r}},9226:(e,t,r)=>{"use strict";r.d(t,{$c:()=>u,Ng:()=>d,RR:()=>c});var n=r(6562),i=r(9557),o=r(9548),a=r(8610),s={regex:/^file:\/\/(.*)/,replacement:"file://OBFUSCATED"};class c extends i.w{constructor(e){super(e)}shouldObfuscate(){return u(this.sharedContext.agentIdentifier).length>0}obfuscateString(e){if(!e||"string"!=typeof e)return e;for(var t=u(this.sharedContext.agentIdentifier),r=e,n=0;n<t.length;n++){var i=t[n].regex,o=t[n].replacement||"*";r=r.replace(i,o)}return r}}function u(e){var t=[],r=(0,n.Mt)(e,"obfuscate")||[];return t=t.concat(r),o.T.isFileProtocol()&&t.push(s),t}function d(e){for(var t=!1,r=!1,n=0;n<e.length;n++){"regex"in e[n]?"string"!=typeof e[n].regex&&e[n].regex.constructor!==RegExp&&((0,a.Z)('An obfuscation replacement rule contains a "regex" value with an invalid type (must be a string or RegExp)'),r=!0):((0,a.Z)('An obfuscation replacement rule was detected missing a "regex" value.'),r=!0);var i=e[n].replacement;i&&"string"!=typeof i&&((0,a.Z)('An obfuscation replacement rule contains a "replacement" value with an invalid type (must be a string)'),t=!0)}return!t&&!r}},2650:(e,t,r)=>{"use strict";r.d(t,{K:()=>a,b:()=>o});var n=r(8544);function i(){return"undefined"==typeof document||"complete"===document.readyState}function o(e,t){if(i())return e();(0,n.bP)("load",e,t)}function a(e){if(i())return e();(0,n.iz)("DOMContentLoaded",e)}},6797:(e,t,r)=>{"use strict";r.d(t,{EZ:()=>u,Qy:()=>c,ce:()=>o,fP:()=>a,gG:()=>d,mF:()=>s});var n=r(2053),i=r(2374);const o={beacon:"bam.nr-data.net",errorBeacon:"bam.nr-data.net"};function a(){return i._A.NREUM||(i._A.NREUM={}),void 0===i._A.newrelic&&(i._A.newrelic=i._A.NREUM),i._A.NREUM}function s(){let e=a();return e.o||(e.o={ST:i._A.setTimeout,SI:i._A.setImmediate,CT:i._A.clearTimeout,XHR:i._A.XMLHttpRequest,REQ:i._A.Request,EV:i._A.Event,PR:i._A.Promise,MO:i._A.MutationObserver,FETCH:i._A.fetch}),e}function c(e,t,r){let i=a();const o=i.initializedAgents||{},s=o[e]||{};return Object.keys(s).length||(s.initializedAt={ms:(0,n.zO)(),date:new Date}),i.initializedAgents={...o,[e]:{...s,[r]:t}},i}function u(e,t){a()[e]=t}function d(){return function(){let e=a();const t=e.info||{};e.info={beacon:o.beacon,errorBeacon:o.errorBeacon,...t}}(),function(){let e=a();const t=e.init||{};e.init={...t}}(),s(),function(){let e=a();const t=e.loader_config||{};e.loader_config={...t}}(),a()}},6998:(e,t,r)=>{"use strict";r.d(t,{N:()=>i,e:()=>o});var n=r(8544);function i(e){let t=arguments.length>1&&void 0!==arguments[1]&&arguments[1];return void(0,n.iz)("visibilitychange",r);function r(){if(t){if("hidden"!=document.visibilityState)return;e()}e(document.visibilityState)}}function o(){return"hidden"===document.visibilityState?-1:1/0}},6034:(e,t,r)=>{"use strict";r.d(t,{t:()=>n});const n=r(2325).D.metrics},2484:(e,t,r)=>{"use strict";r.d(t,{t:()=>n});const n=r(2325).D.pageViewEvent},6382:(e,t,r)=>{"use strict";r.d(t,{t:()=>n});const n=r(2325).D.pageViewTiming},1509:(e,t,r)=>{"use strict";r.d(t,{W:()=>s});var n=r(6562),i=r(3752),o=r(2384),a=r(6797);class s{constructor(e,t,r){this.agentIdentifier=e,this.aggregator=t,this.ee=i.ee.get(e,(0,n.OP)(this.agentIdentifier).isolatedBacklog),this.featureName=r,this.blocked=!1,this.checkConfiguration()}checkConfiguration(){if(!(0,n.lF)(this.agentIdentifier)){let e={...(0,a.gG)().info?.jsAttributes};try{e={...e,...(0,n.C5)(this.agentIdentifier)?.jsAttributes}}catch(e){}(0,o.j)(this.agentIdentifier,{...(0,a.gG)(),info:{...(0,a.gG)().info,jsAttributes:e}})}}}},2384:(e,t,r)=>{"use strict";r.d(t,{j:()=>w});var n=r(8683),i=r.n(n),o=r(2325),a=r(6562),s=r(9252),c=r(7022),u=r(3752),d=r(2053),l=r(4329),f=r(2650),g=r(2374);function h(e,t,n){n||(0,l.R)(e,"api"),function(e){function t(t){for(var r=arguments.length,n=new Array(r>1?r-1:0),i=1;i<r;i++)n[i-1]=arguments[i];Object.values(e.initializedAgents).forEach((e=>{e.exposed&&e.api[t]&&e.api[t](...n)}))}["setErrorHandler","finished","addToTrace","inlineHit","addRelease","addPageAction","setCurrentRouteName","setPageViewName","setCustomAttribute","interaction","noticeError"].forEach((r=>{e[r]=function(){for(var e=arguments.length,n=new Array(e),i=0;i<e;i++)n[i]=arguments[i];return t(r,...n)}}))}(t);var h=u.ee.get(e),p=h.get("tracer"),v="api-",m="api-ixn-";function b(){}(0,c.D)(["setErrorHandler","finished","addToTrace","inlineHit","addRelease"],(function(e,r){t[r]=y(v,r,!0,"api")})),t.addPageAction=y(v,"addPageAction",!0,o.D.pageAction),t.setCurrentRouteName=y(v,"routeName",!0,o.D.spa),t.setPageViewName=function(t,r){if("string"==typeof t)return"/"!==t.charAt(0)&&(t="/"+t),(0,a.OP)(e).customTransaction=(r||"https://web.archive.org/web/20230208102344/http://custom.transaction")+t,y(v,"setPageViewName",!0,"api")()},t.setCustomAttribute=function(t,r){const n=(0,a.C5)(e);return(0,a.CX)(e,{...n,jsAttributes:{...n.jsAttributes,[t]:r}}),y(v,"setCustomAttribute",!0,"api")()},t.interaction=function(){return(new b).get()};var w=b.prototype={createTracer:function(e,t){var r={},n=this,i="function"==typeof t;return(0,s.p)("api-ixn-tracer",[(0,d.zO)(),e,r],n,o.D.spa,h),function(){if(p.emit((i?"":"no-")+"fn-start",[(0,d.zO)(),n,i],r),i)try{return t.apply(this,arguments)}catch(e){throw p.emit("fn-err",[arguments,this,"string"==typeof e?new Error(e):e],r),e}finally{p.emit("fn-end",[(0,d.zO)()],r)}}}};function y(e,t,r,n){return function(){return(0,s.p)("record-supportability",["API/"+t+"/called"],void 0,o.D.metrics,h),(0,s.p)(e+t,[(0,d.zO)()].concat(i()(arguments)),r?null:this,n,h),r?void 0:this}}function A(){r.e(692).then(r.bind(r,5692)).then((t=>{let{setAPI:r}=t;r(e),(0,l.L)(e,"api")}))}(0,c.D)("actionText,setName,setAttribute,save,ignore,onEnd,getContext,end,get".split(","),(function(e,t){w[t]=y(m,t,void 0,o.D.spa)})),t.noticeError=function(e,t){"string"==typeof e&&(e=new Error(e)),(0,s.p)("record-supportability",["API/noticeError/called"],void 0,o.D.metrics,h),(0,s.p)("err",[e,(0,d.zO)(),!1,t],void 0,o.D.jserrors,h)},g.v6?A():(0,f.b)((()=>A()),!0)}var p=r(6797);const v={stn:o.D.sessionTrace,err:o.D.jserrors,ins:o.D.pageAction,spa:o.D.spa};function m(e,t){var r=u.ee.get(t);e&&"object"==typeof e&&((0,c.D)(e,(function(e,t){if(!t)return(0,s.p)("block-"+e,[],void 0,v[e],r);t&&!b[e]&&((0,s.p)("feat-"+e,[],void 0,v[e],r),b[e]=!0)})),(0,l.L)(t,o.D.pageViewEvent))}const b={};function w(e){let t=arguments.length>1&&void 0!==arguments[1]?arguments[1]:{},r=arguments.length>2?arguments[2]:void 0,n=arguments.length>3?arguments[3]:void 0,{init:i,info:o,loader_config:s,runtime:c={loaderType:r},exposed:u=!0}=t;const d=(0,p.gG)();let l={};return o||(i=d.init,o=d.info,s=d.loader_config,l=d),g.v6&&(o.jsAttributes={...o.jsAttributes,isWorker:!0}),(0,a.CX)(e,o),(0,a.Dg)(e,i||{}),(0,a.GE)(e,s||{}),(0,a.sU)(e,c),h(e,l,n),(0,p.Qy)(e,d,"api"),(0,p.Qy)(e,u,"exposed"),g.v6||((0,p.EZ)("activatedFeatures",b),(0,p.EZ)("setToken",(t=>m(t,e)))),l}},909:(e,t,r)=>{"use strict";r.d(t,{Z:()=>i,q:()=>o});var n=r(2325);function i(e){switch(e){case n.D.ajax:return[n.D.jserrors];case n.D.sessionTrace:return[n.D.ajax,n.D.pageViewEvent];case n.D.pageViewTiming:return[n.D.pageViewEvent];default:return[]}}function o(e){return e===n.D.jserrors?[]:["auto"]}},2325:(e,t,r)=>{"use strict";r.d(t,{D:()=>n,p:()=>i});const n={ajax:"ajax",jserrors:"jserrors",metrics:"metrics",pageAction:"page_action",pageViewEvent:"page_view_event",pageViewTiming:"page_view_timing",sessionTrace:"session_trace",spa:"spa"},i={[n.pageViewEvent]:1,[n.pageViewTiming]:2,[n.metrics]:3,[n.jserrors]:4,[n.ajax]:5,[n.sessionTrace]:6,[n.pageAction]:7,[n.spa]:8}},8683:e=>{e.exports=function(e,t,r){t||(t=0),void 0===r&&(r=e?e.length:0);for(var n=-1,i=r-t||0,o=Array(i<0?0:i);++n<i;)o[n]=e[t+n];return o}},4992:(e,t,r)=>{var n={"./ajax/aggregate":[960,779,960],"./jserrors/aggregate":[6112,779,112],"./metrics/aggregate":[4325,779,325],"./page_action/aggregate":[6817,779,817],"./page_view_event/aggregate":[4823,779,823],"./page_view_timing/aggregate":[8785,779,785],"./session_trace/aggregate":[1307,779,307],"./spa/aggregate":[9378,779,378]};function i(e){if(!r.o(n,e))return Promise.resolve().then((()=>{var t=new Error("Cannot find module '"+e+"'");throw t.code="MODULE_NOT_FOUND",t}));var t=n[e],i=t[0];return Promise.all(t.slice(1).map(r.e)).then((()=>r(i)))}i.keys=()=>Object.keys(n),i.id=4992,e.exports=i}},n={};function i(e){var t=n[e];if(void 0!==t)return t.exports;var o=n[e]={exports:{}};return r[e](o,o.exports,i),o.exports}i.m=r,i.n=e=>{var t=e&&e.__esModule?()=>e.default:()=>e;return i.d(t,{a:t}),t},i.d=(e,t)=>{for(var r in t)i.o(t,r)&&!i.o(e,r)&&Object.defineProperty(e,r,{enumerable:!0,get:t[r]})},i.f={},i.e=e=>Promise.all(Object.keys(i.f).reduce(((t,r)=>(i.f[r](e,t),t)),[])),i.u=e=>e+"."+i.h().slice(0,8)+"-1223.js",i.h=()=>"215647de1097144af948",i.o=(e,t)=>Object.prototype.hasOwnProperty.call(e,t),e={},t="NRBA:",i.l=(r,n,o,a)=>{if(e[r])e[r].push(n);else{var s,c;if(void 0!==o)for(var u=document.getElementsByTagName("script"),d=0;d<u.length;d++){var l=u[d];if(l.getAttribute("src")==r||l.getAttribute("data-webpack")==t+o){s=l;break}}s||(c=!0,(s=document.createElement("script")).charset="utf-8",s.timeout=120,i.nc&&s.setAttribute("nonce",i.nc),s.setAttribute("data-webpack",t+o),s.src=r),e[r]=[n];var f=(t,n)=>{s.onerror=s.onload=null,clearTimeout(g);var i=e[r];if(delete e[r],s.parentNode&&s.parentNode.removeChild(s),i&&i.forEach((e=>e(n))),t)return t(n)},g=setTimeout(f.bind(null,void 0,{type:"timeout",target:s}),12e4);s.onerror=f.bind(null,s.onerror),s.onload=f.bind(null,s.onload),c&&document.head.appendChild(s)}},i.r=e=>{"undefined"!=typeof Symbol&&Symbol.toStringTag&&Object.defineProperty(e,Symbol.toStringTag,{value:"Module"}),Object.defineProperty(e,"__esModule",{value:!0})},i.p="https://web.archive.org/web/20230208102344/https://js-agent.newrelic.com/",(()=>{var e={55:0,308:0};i.f.j=(t,r)=>{var n=i.o(e,t)?e[t]:void 0;if(0!==n)if(n)r.push(n[2]);else{var o=new Promise(((r,i)=>n=e[t]=[r,i]));r.push(n[2]=o);var a=i.p+i.u(t),s=new Error;i.l(a,(r=>{if(i.o(e,t)&&(0!==(n=e[t])&&(e[t]=void 0),n)){var o=r&&("load"===r.type?"missing":r.type),a=r&&r.target&&r.target.src;s.message="Loading chunk "+t+" failed.\n("+o+": "+a+")",s.name="ChunkLoadError",s.type=o,s.request=a,n[1](s)}}),"chunk-"+t,t)}};var t=(t,r)=>{var n,o,[a,s,c]=r,u=0;if(a.some((t=>0!==e[t]))){for(n in s)i.o(s,n)&&(i.m[n]=s[n]);if(c)c(i)}for(t&&t(r);u<a.length;u++)o=a[u],i.o(e,o)&&e[o]&&e[o][0](),e[o]=0},r=window.webpackChunkNRBA=window.webpackChunkNRBA||[];r.forEach(t.bind(null,0)),r.push=t.bind(null,r.push.bind(r))})();var o={};(()=>{"use strict";i.r(o);var e=i(2325),t=i(6562);const r=Object.values(e.D);function n(e){const n={};return r.forEach((r=>{n[r]=function(e,r){return!1!==(0,t.Mt)(r,`${e}.enabled`)}(r,e)})),n}var a=i(2384),s=i(909),c=i(9557),u=i(7022);class d extends c.w{constructor(e){super(e),this.aggregatedData={}}store(e,t,r,n,i){var o=this.getBucket(e,t,r,i);return o.metrics=function(e,t){t||(t={count:0});return t.count+=1,(0,u.D)(e,(function(e,r){t[e]=l(r,t[e])})),t}(n,o.metrics),o}merge(e,t,r,n,i){var o=this.getBucket(e,t,n,i);if(o.metrics){var a=o.metrics;a.count+=r.count,(0,u.D)(r,(function(e,t){if("count"!==e){var n=a[e],i=r[e];i&&!i.c?a[e]=l(i.t,n):a[e]=function(e,t){if(!t)return e;t.c||(t=f(t.t));return t.min=Math.min(e.min,t.min),t.max=Math.max(e.max,t.max),t.t+=e.t,t.sos+=e.sos,t.c+=e.c,t}(i,a[e])}}))}else o.metrics=r}storeMetric(e,t,r,n){var i=this.getBucket(e,t,r);return i.stats=l(n,i.stats),i}getBucket(e,t,r,n){this.aggregatedData[e]||(this.aggregatedData[e]={});var i=this.aggregatedData[e][t];return i||(i=this.aggregatedData[e][t]={params:r||{}},n&&(i.custom=n)),i}get(e,t){return t?this.aggregatedData[e]&&this.aggregatedData[e][t]:this.aggregatedData[e]}take(e){for(var t={},r="",n=!1,i=0;i<e.length;i++)t[r=e[i]]=g(this.aggregatedData[r]),t[r].length&&(n=!0),delete this.aggregatedData[r];return n?t:null}}function l(e,t){return null==e?function(e){e?e.c++:e={c:1};return e}(t):t?(t.c||(t=f(t.t)),t.c+=1,t.t+=e,t.sos+=e*e,e>t.max&&(t.max=e),e<t.min&&(t.min=e),t):{t:e}}function f(e){return{t:e,min:e,max:e,sos:e*e,c:1}}function g(e){return"object"!=typeof e?[]:(0,u.D)(e,h)}function h(e,t){return t}var p=i(6797),v=i(5526),m=i(8610);var b=i(9252),w=i(2053),y=i(8283),A=i(6625),x=i(4329),E=i(1509),O=i(2650),S=i(2374);class D extends E.W{constructor(e,t,r){let n=!(arguments.length>3&&void 0!==arguments[3])||arguments[3];super(e,t,r),this.completed=new Promise(((e,t)=>{this.resolve=e,this.reject=t})),this.hasAggregator=!1,this.auto=n,n&&(0,x.R)(e,r)}importAggregator(){try{if(this.hasAggregator||!this.auto)return;this.hasAggregator=!0;const e=async()=>{try{const{Aggregate:e}=await i(4992)(`./${this.featureName}/aggregate`);new e(this.agentIdentifier,this.aggregator),this.resolve()}catch(e){(0,m.Z)(`Failed to import aggregator class for ${this.featureName}`,e)}};S.v6?e():(0,O.b)((()=>e()),!0)}catch(e){this.reject(e)}}}var j,_,k,T=i(2484);class P extends D{constructor(e,t){let r=!(arguments.length>2&&void 0!==arguments[2])||arguments[2];super(e,t,T.t,r),S.il&&((0,A.v)(e),(0,y.B)(e,"firstbyte",(0,w.yf)()),(0,O.K)((()=>this.measureDomContentLoaded())),(0,O.b)((()=>this.measureWindowLoaded()),!0),this.importAggregator())}measureWindowLoaded(){var t=(0,w.zO)();(0,y.B)(this.agentIdentifier,"onload",t+(0,w.os)()),(0,b.p)("timing",["load",t],void 0,e.D.pageViewTiming,this.ee)}measureDomContentLoaded(){(0,y.B)(this.agentIdentifier,"domContent",(0,w.zO)()+(0,w.os)())}}j=P,_="featureName",k=T.t,_ in j?Object.defineProperty(j,_,{value:k,enumerable:!0,configurable:!0,writable:!0}):j[_]=k;var C=i(6998),I=i(8544),N=i(6382);class R extends D{constructor(t,r){var n;let i=!(arguments.length>2&&void 0!==arguments[2])||arguments[2];if(super(t,r,N.t,i),n=this,S.il){if(this.pageHiddenTime=(0,C.e)(),this.performanceObserver,this.lcpPerformanceObserver,this.clsPerformanceObserver,this.fiRecorded=!1,"PerformanceObserver"in window&&"function"==typeof window.PerformanceObserver){this.performanceObserver=new PerformanceObserver((function(){return n.perfObserver(...arguments)}));try{this.performanceObserver.observe({entryTypes:["paint"]})}catch(e){}this.lcpPerformanceObserver=new PerformanceObserver((function(){return n.lcpObserver(...arguments)}));try{this.lcpPerformanceObserver.observe({entryTypes:["largest-contentful-paint"]})}catch(e){}this.clsPerformanceObserver=new PerformanceObserver((function(){return n.clsObserver(...arguments)}));try{this.clsPerformanceObserver.observe({type:"layout-shift",buffered:!0})}catch(e){}}this.fiRecorded=!1;["click","keydown","mousedown","pointerdown","touchstart"].forEach((e=>{(0,I.iz)(e,(function(){return n.captureInteraction(...arguments)}))})),(0,C.N)((()=>{this.pageHiddenTime=(0,w.zO)(),(0,b.p)("docHidden",[this.pageHiddenTime],void 0,e.D.pageViewTiming,this.ee)}),!0),(0,I.bP)("pagehide",(()=>(0,b.p)("winPagehide",[(0,w.zO)()],void 0,e.D.pageViewTiming,this.ee))),this.importAggregator()}}perfObserver(t,r){t.getEntries().forEach((t=>{"first-paint"===t.name?(0,b.p)("timing",["fp",Math.floor(t.startTime)],void 0,e.D.pageViewTiming,this.ee):"first-contentful-paint"===t.name&&(0,b.p)("timing",["fcp",Math.floor(t.startTime)],void 0,e.D.pageViewTiming,this.ee)}))}lcpObserver(t,r){var n=t.getEntries();if(n.length>0){var i=n[n.length-1];if(this.pageHiddenTime<i.startTime)return;var o=[i],a=this.addConnectionAttributes({});a&&o.push(a),(0,b.p)("lcp",o,void 0,e.D.pageViewTiming,this.ee)}}clsObserver(t){t.getEntries().forEach((t=>{t.hadRecentInput||(0,b.p)("cls",[t],void 0,e.D.pageViewTiming,this.ee)}))}addConnectionAttributes(e){var t=navigator.connection||navigator.mozConnection||navigator.webkitConnection;if(t)return t.type&&(e["net-type"]=t.type),t.effectiveType&&(e["net-etype"]=t.effectiveType),t.rtt&&(e["net-rtt"]=t.rtt),t.downlink&&(e["net-dlink"]=t.downlink),e}captureInteraction(r){if(r instanceof t.Yu.EV&&!this.fiRecorded){var n=Math.round(r.timeStamp),i={type:r.type};this.addConnectionAttributes(i),n<=(0,w.zO)()?i.fid=(0,w.zO)()-n:n>(0,w.os)()&&n<=Date.now()?(n-=(0,w.os)(),i.fid=(0,w.zO)()-n):n=(0,w.zO)(),this.fiRecorded=!0,(0,b.p)("timing",["fi",n,i],void 0,e.D.pageViewTiming,this.ee)}}}!function(e,t,r){t in e?Object.defineProperty(e,t,{value:r,enumerable:!0,configurable:!0,writable:!0}):e[t]=r}(R,"featureName",N.t);var B=i(4045),M="React",V="Angular",q="AngularJS",L="Backbone",z="Ember",W="Vue",$="Meteor",F="Zepto",G="Jquery";function Z(){if(!S.il)return[];var e=[];try{(function(){try{if(window.React||window.ReactDOM||window.ReactRedux)return!0;if(document.querySelector("[data-reactroot], [data-reactid]"))return!0;for(var e=document.querySelectorAll("body > div"),t=0;t<e.length;t++)if(Object.keys(e[t]).indexOf("_reactRootContainer")>=0)return!0;return!1}catch(e){return!1}})()&&e.push(M),function(){try{return!!window.angular||(!!document.querySelector(".ng-binding, [ng-app], [data-ng-app], [ng-controller], [data-ng-controller], [ng-repeat], [data-ng-repeat]")||!!document.querySelector('script[src*="angular.js"], script[src*="angular.min.js"]'))}catch(e){return!1}}()&&e.push(q),function(){try{return!!(window.hasOwnProperty("ng")&&window.ng.hasOwnProperty("coreTokens")&&window.ng.coreTokens.hasOwnProperty("NgZone"))||!!document.querySelectorAll("[ng-version]").length}catch(e){return!1}}()&&e.push(V),window.Backbone&&e.push(L),window.Ember&&e.push(z),window.Vue&&e.push(W),window.Meteor&&e.push($),window.Zepto&&e.push(F),window.jQuery&&e.push(G)}catch(e){}return e}var U=i(9548),H=i(9226),X=i(8226);const Q=Boolean(S._A?.Worker),K=Boolean(S._A?.SharedWorker),J=Boolean(S._A?.navigator?.serviceWorker);let Y,ee,te;var re=i(6034);class ne extends D{constructor(e,t){var r;let n=arguments.length>2&&void 0!==arguments[2]?arguments[2]:{},i=!(arguments.length>3&&void 0!==arguments[3])||arguments[3];super(e,t,re.t,i),r=this,this.PfFeatStatusEnum=n,this.singleChecks(),this.eachSessionChecks(),(0,B.X)("record-supportability",(function(){return r.recordSupportability(...arguments)}),this.featureName,this.ee),(0,B.X)("record-custom",(function(){return r.recordCustom(...arguments)}),this.featureName,this.ee),this.importAggregator()}recordSupportability(e,t){var r=["sm",e,{name:e},t];return(0,b.p)("storeMetric",r,null,this.featureName,this.ee),r}recordCustom(e,t){var r=["cm",e,{name:e},t];return(0,b.p)("storeEventMetrics",r,null,this.featureName,this.ee),r}singleChecks(){this.recordSupportability(`Generic/Version/${X.q}/Detected`);const{loaderType:e}=(0,t.OP)(this.agentIdentifier);e&&this.recordSupportability(`Generic/LoaderType/${e}/Detected`),S.il&&(0,O.K)((()=>{Z().forEach((e=>{this.recordSupportability("Framework/"+e+"/Detected")}))})),U.T.isFileProtocol()&&(this.recordSupportability("Generic/FileProtocol/Detected"),U.T.supportabilityMetricSent=!0);const r=(0,H.$c)(this.agentIdentifier);r.length>0&&this.recordSupportability("Generic/Obfuscate/Detected"),r.length>0&&!(0,H.Ng)(r)&&this.recordSupportability("Generic/Obfuscate/Invalid"),S.il&&this.reportPolyfillsNeeded(),function(e){if(!Y){if(Q){Y=Worker;try{S._A.Worker=r(Y,"Dedicated")}catch(e){o(e,"Dedicated")}if(K){ee=SharedWorker;try{S._A.SharedWorker=r(ee,"Shared")}catch(e){o(e,"Shared")}}else n("Shared");if(J){te=navigator.serviceWorker.register;try{S._A.navigator.serviceWorker.register=(t=te,function(){for(var e=arguments.length,r=new Array(e),n=0;n<e;n++)r[n]=arguments[n];return i("Service",r[1]?.type),t.apply(navigator.serviceWorker,r)})}catch(e){o(e,"Service")}}else n("Service");var t;return}n("All")}function r(e,t){return new Proxy(e,{construct:(e,r)=>(i(t,r[1]?.type),new e(...r))})}function n(t){S.v6||e(`Workers/${t}/Unavailable`)}function i(t,r){e("module"===r?`Workers/${t}/Module`:`Workers/${t}/Classic`)}function o(t,r){e(`Workers/${r}/SM/Unsupported`),(0,m.Z)(`NR Agent: Unable to capture ${r} workers.`,t)}}(this.recordSupportability.bind(this))}reportPolyfillsNeeded(){this.recordSupportability(`Generic/Polyfill/Promise/${this.PfFeatStatusEnum.PROMISE}`),this.recordSupportability(`Generic/Polyfill/ArrayIncludes/${this.PfFeatStatusEnum.ARRAY_INCLUDES}`),this.recordSupportability(`Generic/Polyfill/ObjectAssign/${this.PfFeatStatusEnum.OBJECT_ASSIGN}`),this.recordSupportability(`Generic/Polyfill/ObjectEntries/${this.PfFeatStatusEnum.OBJECT_ENTRIES}`)}eachSessionChecks(){S.il&&(0,I.bP)("pageshow",(e=>{e.persisted&&this.recordCustom("Custom/BFCache/PageRestored")}))}}!function(e,t,r){t in e?Object.defineProperty(e,t,{value:r,enumerable:!0,configurable:!0,writable:!0}):e[t]=r}(ne,"featureName",re.t);new class{constructor(t){let r=arguments.length>1&&void 0!==arguments[1]?arguments[1]:(0,v.ky)(16);this.agentIdentifier=r,this.sharedAggregator=new d({agentIdentifier:this.agentIdentifier}),this.features={},this.desiredFeatures=t.features||[],this.desiredFeatures.sort(((t,r)=>e.p[t.featureName]-e.p[r.featureName])),Object.assign(this,(0,a.j)(this.agentIdentifier,t,t.loaderType||"agent")),this.start()}get config(){return{info:(0,t.C5)(this.agentIdentifier),init:(0,t.P_)(this.agentIdentifier),loader_config:(0,t.DL)(this.agentIdentifier),runtime:(0,t.OP)(this.agentIdentifier)}}start(){try{const e=n(this.agentIdentifier);this.desiredFeatures.forEach((t=>{if(e[t.featureName]){const r=(0,s.Z)(t.featureName);r.every((t=>e[t]))||(0,m.Z)(`${t.featureName} is enabled but one or more dependent features has been disabled (${JSON.stringify(r)}). This may cause unintended consequences or missing data...`),this.features[t.featureName]=new t(this.agentIdentifier,this.sharedAggregator)}})),(0,p.Qy)(this.agentIdentifier,this.features,"features")}catch(e){return(0,m.Z)("Failed to initialize instrument classes",e),!1}}}({features:[P,R,ne],loaderType:"lite"})})(),window.NRBA=o})();</script> <noscript><style>form.antibot * :not(.antibot-message) { display: none !important; }</style> </noscript><script async src="https://web.archive.org/web/20230208102344js_/https://www.googletagmanager.com/gtag/js?id=UA-66610693-3"></script> <script>window.dataLayer = window.dataLayer || [];function gtag(){dataLayer.push(arguments)};gtag("js", new Date());gtag("set", "developer_id.dMDhkMT", true);gtag('set', {cookie_flags: 'SameSite=None;Secure'});gtag("config", "UA-66610693-3", {"groups":"default","anonymize_ip":true,"page_placeholder":"PLACEHOLDER_page_path","link_attribution":true,"allow_ad_personalization_signals":false});gtag("config", "G-HEQ0YF2VYL", {"groups":"default","page_placeholder":"PLACEHOLDER_page_location","link_attribution":true,"allow_ad_personalization_signals":false});gtag("config", "UA-33523145-1", {"groups":"default","anonymize_ip":true,"page_placeholder":"PLACEHOLDER_page_path","link_attribution":true,"allow_ad_personalization_signals":false});</script> <link rel="canonical" href="https://web.archive.org/web/20230208102344/https://www.nist.gov/image/moleculespng"/> <link rel="shortlink" href="https://web.archive.org/web/20230208102344/https://www.nist.gov/media/485396"/> <meta name="description" content="In this image, 200 nanometers (nm, billionths of a meter) square image, the size and direction of the arrows show the average local alignment of individual fluorescent molecules as a result of the strain produced by a square punch (the size of the gray dotted line) driven into the material, and then"/> <meta name="keywords" content="Quality assurance, Composites, Materials characterization, Force metrology, Nanomaterials, Nanometrology, Optical physics and communications, Disaster and failure studies"/> <meta name="dcterms.title" content="individual fluorescent molecules"/> <meta name="dcterms.description" content="In this image, 200 nanometers (nm, billionths of a meter) square image, the size and direction of the arrows show the average local alignment of individual fluorescent molecules as a result of the strain produced by a square punch (the size of the gray dotted line) driven into the material, and then"/> <meta name="dcterms.type" content="Image"/> <meta name="dcterms.format" content="image/png"/> <meta name="dcterms.identifier" content="https://www.nist.gov/image/moleculespng"/> <meta name="dcterms.source" content="NIST"/> <meta name="citation_title" content="individual fluorescent molecules | NIST"/> <meta property="og:site_name" content="NIST"/> <meta property="og:type" content="Article"/> <meta property="og:url" content="https://web.archive.org/web/20230208102344/https://www.nist.gov/image/moleculespng"/> <meta property="og:title" content="individual fluorescent molecules"/> <meta property="og:description" content="In this image, 200 nanometers (nm, billionths of a meter) square image, the size and direction of the arrows show the average local alignment of individual fluorescent molecules as a result of the strain produced by a square punch (the size of the gray dotted line) driven into the material, and then"/> <meta property="og:image" content="https://web.archive.org/web/20230208102344im_/https://www.nist.gov/sites/default/files/images/2019/02/25/molecules.png"/> <meta property="og:image:type" content="image/png"/> <meta name="google-site-verification" content="QMu0ODkER3rN5hLcMLqNVf7e3bkjYsNLTuhqfH48jCA"/> <meta name="twitter:card" content="summary_large_image"/> <meta name="twitter:site" content="NIST"/> <meta name="twitter:description" content="In this image, 200 nanometers (nm, billionths of a meter) square image, the size and direction of the arrows show the average local alignment of individual fluorescent molecules as a result of the strain produced by a square punch (the size of the gray dotted line) driven into the material, and then"/> <meta name="twitter:title" content="individual fluorescent molecules"/> <meta name="twitter:url" content="https://web.archive.org/web/20230208102344im_/https://www.nist.gov/image/moleculespng"/> <meta name="twitter:image" content="https://web.archive.org/web/20230208102344im_/https://www.nist.gov/sites/default/files/images/2019/02/25/molecules.png"/> <meta name="Generator" content="Drupal 9 (https://www.drupal.org)"/> <meta name="MobileOptimized" content="width"/> <meta name="HandheldFriendly" content="true"/> <meta name="viewport" content="width=device-width, initial-scale=1.0"/> <script type="application/ld+json">{ "@context": "https://web.archive.org/web/20230208102344/https://schema.org", "@graph": [ { "@type": "Article", "headline": "individual fluorescent molecules | NIST" } ] }</script> <meta name="nist_search_bundle" class="elastic" content="image"/> <link rel="icon" href="/web/20230208102344im_/https://www.nist.gov/themes/custom/nist_www/favicon.ico" type="image/vnd.microsoft.icon"/> <title>individual fluorescent molecules</title> <link rel="stylesheet" media="all" href="/web/20230208102344cs_/https://www.nist.gov/sites/default/files/css/css_6IARttm8vyVGJNHC35WOrBrHZqtrgyElXXf3aKPkCpU.css"/> <link rel="stylesheet" media="screen" href="/web/20230208102344cs_/https://www.nist.gov/sites/default/files/css/css_gD8QmGaNfUQuThtjB-S0EwEOZZNJP1QfUU1bDdLHbMU.css"/> <link rel="stylesheet" media="print" href="/web/20230208102344cs_/https://www.nist.gov/sites/default/files/css/css_zbjDhj-AmeVwgu9y0DlPwEfp7l-a5BpoK3MpoGmRB6o.css"/> <link rel="stylesheet" media="all" href="/web/20230208102344cs_/https://www.nist.gov/sites/default/files/css/css_PV0KmreUx3hVeYI6yGypf71UDz9BSQl1Z5DjiESDh-Y.css"/> <link rel="stylesheet" media="screen" href="/web/20230208102344cs_/https://www.nist.gov/sites/default/files/css/css_h014k13aNG5kxmc3i31BCnMD2Gr39UKWutWZuQuCXMo.css"/> <script src="/web/20230208102344js_/https://www.nist.gov/sites/default/files/js/js_VDwaIMNiRtzQn-NZvj1F9loIzJ-i4yEDiruzS8Z6Ejo.js"></script> </head> <body> <a href="#main-content" class="visually-hidden focusable" data-elastic-exclude> Skip to main content </a> <div class="dialog-off-canvas-main-canvas" data-off-canvas-main-canvas> <!-- nist-index-ignore-start --> <section data-elastic-exclude class="usa-banner" aria-label="Official government website"> <div class="usa-accordion"> <header class="usa-banner__header"> <div class="usa-banner__inner"> <div class="grid-col-auto"> <img class="usa-banner__header-flag" src="/web/20230208102344im_/https://www.nist.gov/libraries/nist-component-library/dist/img/us_flag_small.png" alt="U.S. flag"> </div> <div class="grid-col-fill tablet:grid-col-auto"> <p class="usa-banner__header-text">An official website of the United States government</p> <p class="usa-banner__header-action" aria-hidden="true">Here鈥檚 how you know</p> </div> <button class="usa-accordion__button usa-banner__button" aria-expanded="false" aria-controls="gov-banner-default"> <span class="usa-banner__button-text">Here鈥檚 how you know</span> </button> </div> </header> <div class="usa-banner__content usa-accordion__content" id="gov-banner-default"> <div class="grid-row grid-gap-lg"> <div class="usa-banner__guidance tablet:grid-col-6"> <img class="usa-banner__icon usa-media-block__img" src="/web/20230208102344im_/https://www.nist.gov/libraries/nist-component-library/dist/img/icon-dot-gov.svg" role="img" alt="" aria-hidden="true"> <div class="usa-media-block__body"> <p> <strong>Official websites use .gov</strong> <br/> A <strong>.gov</strong> website belongs to an official government organization in the United States. </p> </div> </div> <div class="usa-banner__guidance tablet:grid-col-6"> <img class="usa-banner__icon usa-media-block__img" src="/web/20230208102344im_/https://www.nist.gov/libraries/nist-component-library/dist/img/icon-https.svg" role="img" alt="" aria-hidden="true"> <div class="usa-media-block__body"> <p> <strong>Secure .gov websites use HTTPS</strong> <br/> A <strong>lock</strong> ( <span class="icon-lock"> <svg xmlns="http://www.w3.org/2000/svg" width="52" height="64" viewbox="0 0 52 64" class="usa-banner__lock-image" role="img" aria-labelledby="banner-lock-title banner-lock-description" focusable="false"> <title id="banner-lock-title">Lock</title> <desc id="banner-lock-description">A locked padlock</desc><path fill="#000000" fill-rule="evenodd" d="M26 0c10.493 0 19 8.507 19 19v9h3a4 4 0 0 1 4 4v28a4 4 0 0 1-4 4H4a4 4 0 0 1-4-4V32a4 4 0 0 1 4-4h3v-9C7 8.507 15.507 0 26 0zm0 8c-5.979 0-10.843 4.77-10.996 10.712L15 19v9h22v-9c0-6.075-4.925-11-11-11z"/></svg> </span> ) or <strong>https://</strong> means you鈥檝e safely connected to the .gov website. Share sensitive information only on official, secure websites. </p> </div> </div> </div> </div> </div> </section> <!-- nist-index-ignore-end --> <div data-elastic-exclude> <!-- nist-index-ignore-start --> <div class="nist-print-header" style="display:none;"> <p class="nist-print-header__url">https://www.nist.gov/image/moleculespng</p> <img class="nist-print-header__logo" width="289" height="38" src="/web/20230208102344im_/https://www.nist.gov/libraries/nist-component-library/dist/img/logo/nist_logo_sidestack.svg" alt="National Institute of Standards and Technology"/> </div> <div class="usa-overlay"></div> <header class="usa-header nist-header--www nist-header--minimal" role="banner"> <div class="usa-navbar"> <div class="usa-logo flex-fill"> <a href="/web/20230208102344/https://www.nist.gov/" title="National Institute of Standards and Technology" aria-label="Home"> <img src="/web/20230208102344im_/https://www.nist.gov/libraries/nist-component-library/dist/img/logo/logo.svg" alt="National Institute of Standards and Technology" width="300px" height="80px"/> </a> </div> <div class="usa-header__right grid-row flex-auto"> <div class="grid-col-fill nist-header__search-group"> <form class="usa-search usa-search--small" accept-charset="UTF-8" action="/web/20230208102344/https://www.nist.gov/search" id="search_form" method="get"> <div role="search"> <label class="usa-sr-only" for="search-form">Search NIST</label> <input class="usa-input" id="search-form" type="search" name="s" placeholder="Search NIST" required="" maxlength="128"> <button class="usa-button" type="submit"><img src="/web/20230208102344im_/https://www.nist.gov/libraries/nist-component-library/dist/img/usa-icons-bg/search--white.svg" class="usa-search__submit-icon" alt="Search"></button> </div> </form> </div> <div class="grid-col-auto padding-left-1"> <button class="usa-menu-btn">Menu</button> </div> </div> </div> <nav aria-label="Primary navigation" class="usa-nav"> <div class="usa-nav__inner"> <button class="usa-nav__close">Close</button> <ul class="usa-nav__primary usa-accordion"> <li class="usa-nav__primary-item"> <button class="usa-accordion__button usa-nav__link" aria-expanded="false" aria-controls="primary_menu-1"><span>Topics</span></button> <div id="primary_menu-1" class="usa-nav__submenu usa-megamenu"> <div class="grid-row"> <div class="tablet:grid-col-fill"><ul class="usa-nav__submenu-list"> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/topics" class="usa-nav__link">All Topics</a> </li> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/advanced-communications" class="usa-nav__link">Advanced communications</a> </li> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/artificial-intelligence" class="usa-nav__link">Artificial intelligence</a> </li> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/bioscience" class="usa-nav__link">Bioscience</a> </li> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/buildings-construction" class="usa-nav__link">Buildings and construction</a> </li> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/chemistry" class="usa-nav__link">Chemistry</a> </li> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/climate" class="usa-nav__link">Climate</a> </li> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/cybersecurity" class="usa-nav__link">Cybersecurity</a> </li> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/electronics" class="usa-nav__link">Electronics</a> </li> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/energy" class="usa-nav__link">Energy</a> </li> </ul></div><div class="column-break tablet:grid-col-fill"><ul class="usa-nav__submenu-list"> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/environment" class="usa-nav__link">Environment</a> </li> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/fire" class="usa-nav__link">Fire</a> </li> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/forensic-science" class="usa-nav__link">Forensic science</a> </li> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/health" class="usa-nav__link">Health</a> </li> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/information-technology" class="usa-nav__link">Information technology</a> </li> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/infrastructure" class="usa-nav__link">Infrastructure</a> </li> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/manufacturing" class="usa-nav__link">Manufacturing</a> </li> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/materials" class="usa-nav__link">Materials</a> </li> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/mathematics-statistics" class="usa-nav__link">Mathematics and statistics</a> </li> </ul></div><div class="column-break tablet:grid-col-fill"><ul class="usa-nav__submenu-list"> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/metrology" class="usa-nav__link">Metrology</a> </li> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/nanotechnology" class="usa-nav__link">Nanotechnology</a> </li> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/neutron-research" class="usa-nav__link">Neutron research</a> </li> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/performance-excellence" class="usa-nav__link">Performance excellence</a> </li> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/physics" class="usa-nav__link">Physics</a> </li> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/public-safety" class="usa-nav__link">Public safety</a> </li> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/resilience" class="usa-nav__link">Resilience</a> </li> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/standards" class="usa-nav__link">Standards</a> </li> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/transportation" class="usa-nav__link">Transportation</a> </li> </ul> </div> </div> </div> </li> <li class="usa-nav__primary-item"> <a class="usa-nav__link" href="/web/20230208102344/https://www.nist.gov/publications">Publications</a> </li> <li class="usa-nav__primary-item"> <button class="usa-accordion__button usa-nav__link" aria-expanded="false" aria-controls="primary_menu-3"><span>Labs &amp; Major Programs</span></button> <div id="primary_menu-3" class="usa-nav__submenu usa-megamenu"> <div class="grid-row"> <div class="tablet:grid-col-fill"><ul class="usa-nav__submenu-list"> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/labs-major-programs/laboratories" class="usa-nav__link">Laboratories</a> <ul class="usa-nav__submenu-list"> <li> <a href="/web/20230208102344/https://www.nist.gov/ctl" class="usa-nav__link">Communications Technology Laboratory</a> </li> <li> <a href="/web/20230208102344/https://www.nist.gov/el" class="usa-nav__link">Engineering Laboratory</a> </li> <li> <a href="/web/20230208102344/https://www.nist.gov/itl" class="usa-nav__link">Information Technology Laboratory</a> </li> <li> <a href="/web/20230208102344/https://www.nist.gov/mml" class="usa-nav__link">Material Measurement Laboratory</a> </li> <li> <a href="/web/20230208102344/https://www.nist.gov/pml" class="usa-nav__link">Physical Measurement Laboratory</a> </li> </ul> </li> </ul></div><div class="column-break tablet:grid-col-fill"><ul class="usa-nav__submenu-list"> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/labs-major-programs/user-facilities" class="usa-nav__link">User Facilities</a> <ul class="usa-nav__submenu-list"> <li> <a href="/web/20230208102344/https://www.nist.gov/ncnr" class="usa-nav__link">NIST Center for Neutron Research</a> </li> <li> <a href="/web/20230208102344/https://www.nist.gov/cnst" class="usa-nav__link">CNST NanoFab</a> </li> </ul> </li> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/labs-major-programs/research-test-beds" class="usa-nav__link">Research Test Beds</a> </li> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/laboratories/projects-programs" class="usa-nav__link">Research Projects</a> </li> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/laboratories/tools-instruments" class="usa-nav__link">Tools &amp; Instruments</a> </li> </ul></div><div class="column-break tablet:grid-col-fill"><ul class="usa-nav__submenu-list"> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/labs-major-programs/major-programs" class="usa-nav__link">Major Programs</a> <ul class="usa-nav__submenu-list"> <li> <a href="/web/20230208102344/https://www.nist.gov/baldrige" class="usa-nav__link">Baldrige Performance Excellence Program</a> </li> <li> <a href="/web/20230208102344/https://www.nist.gov/chips" class="usa-nav__link">CHIPS for America Initiative</a> </li> <li> <a href="/web/20230208102344/https://www.nist.gov/mep" class="usa-nav__link">Manufacturing Extension Partnership (MEP)</a> </li> <li> <a href="/web/20230208102344/https://www.nist.gov/oam" class="usa-nav__link">Office of Advanced Manufacturing</a> </li> <li> <a href="/web/20230208102344/https://www.nist.gov/spo" class="usa-nav__link">Special Programs Office</a> </li> <li> <a href="/web/20230208102344/https://www.nist.gov/tpo" class="usa-nav__link">Technology Partnerships Office</a> </li> </ul> </li> </ul> </div> </div> </div> </li> <li class="usa-nav__primary-item"> <button class="usa-accordion__button usa-nav__link" aria-expanded="false" aria-controls="primary_menu-4"><span>Services &amp; Resources</span></button> <div id="primary_menu-4" class="usa-nav__submenu usa-megamenu"> <div class="grid-row"> <div class="tablet:grid-col-fill"><ul class="usa-nav__submenu-list"> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/services-resources/standards-and-measurements" class="usa-nav__link">Standards and Measurements</a> <ul class="usa-nav__submenu-list"> <li> <a href="/web/20230208102344/https://www.nist.gov/calibrations" class="usa-nav__link">Calibration Services</a> </li> <li> <a href="/web/20230208102344/https://www.nist.gov/nvlap" class="usa-nav__link">Laboratory Accreditation (NVLAP)</a> </li> <li> <a href="/web/20230208102344/https://www.nist.gov/nist-quality-system" class="usa-nav__link">Quality System</a> </li> <li> <a href="/web/20230208102344/https://www.nist.gov/srm" class="usa-nav__link">Standard Reference Materials (SRMs)</a> </li> <li> <a href="/web/20230208102344/https://www.nist.gov/standardsgov" class="usa-nav__link">Standards.gov</a> </li> <li> <a href="/web/20230208102344/https://www.nist.gov/pml/time-and-frequency-division/time-services" class="usa-nav__link">Time Services</a> </li> <li> <a href="/web/20230208102344/https://www.nist.gov/pml/owm" class="usa-nav__link">Office of Weights and Measures</a> </li> </ul> </li> </ul></div><div class="column-break tablet:grid-col-fill"><ul class="usa-nav__submenu-list"> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/services-resources/software" class="usa-nav__link">Software</a> </li> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/data" class="usa-nav__link">Data</a> <ul class="usa-nav__submenu-list"> <li> <a href="https://web.archive.org/web/20230208102344/https://webbook.nist.gov/chemistry/" class="usa-nav__link">Chemistry WebBook</a> </li> <li> <a href="https://web.archive.org/web/20230208102344/https://nvd.nist.gov/" class="usa-nav__link">National Vulnerability Database</a> </li> <li> <a href="/web/20230208102344/https://www.nist.gov/pml/productsservices/physical-reference-data" class="usa-nav__link">Physical Reference Data</a> </li> <li> <a href="/web/20230208102344/https://www.nist.gov/srd" class="usa-nav__link">Standard Reference Data (SRD)</a> </li> </ul> </li> <li class="usa-nav__submenu-item"> <a href="https://web.archive.org/web/20230208102344/https://www.nist.gov/shop" class="usa-nav__link">Storefront</a> </li> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/tpo" class="usa-nav__link">License &amp; Patents</a> </li> </ul></div><div class="column-break tablet:grid-col-fill"><ul class="usa-nav__submenu-list"> <li class="usa-nav__submenu-item"> <a href="https://web.archive.org/web/20230208102344/https://csrc.nist.gov/" class="usa-nav__link">Computer Security Resource Center (CSRC)</a> </li> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/nist-research-library" class="usa-nav__link">NIST Research Library</a> </li> </ul> </div> </div> </div> </li> <li class="usa-nav__primary-item"> <button class="usa-accordion__button usa-nav__link" aria-expanded="false" aria-controls="primary_menu-5"><span>News &amp; Events</span></button> <div id="primary_menu-5" class="usa-nav__submenu usa-megamenu"> <div class="grid-row"> <div class="tablet:grid-col-fill"><ul class="usa-nav__submenu-list"> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/news-events/news" class="usa-nav__link">News</a> </li> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/news-events/events" class="usa-nav__link">Events</a> </li> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/blogs" class="usa-nav__link">Blogs</a> </li> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/feature-stories" class="usa-nav__link">Feature Stories</a> </li> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/awards" class="usa-nav__link">Awards</a> </li> </ul></div><div class="column-break tablet:grid-col-fill"><ul class="usa-nav__submenu-list"> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/video-gallery" class="usa-nav__link">Video Gallery</a> </li> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/image-gallery" class="usa-nav__link">Image Gallery</a> </li> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/director/pao/media-contacts" class="usa-nav__link">Media Contacts</a> </li> </ul> </div> </div> </div> </li> <li class="usa-nav__primary-item"> <button class="usa-accordion__button usa-nav__link" aria-expanded="false" aria-controls="primary_menu-6"><span>About NIST</span></button> <div id="primary_menu-6" class="usa-nav__submenu usa-megamenu"> <div class="grid-row"> <div class="tablet:grid-col-fill"><ul class="usa-nav__submenu-list"> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/about-nist" class="usa-nav__link">About Us</a> </li> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/about-nist/contact-us" class="usa-nav__link">Contact Us</a> </li> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/about-nist/visit" class="usa-nav__link">Visit</a> </li> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/careers" class="usa-nav__link">Careers</a> </li> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/director/nist-organization-structure" class="usa-nav__link">Our Organization</a> <ul class="usa-nav__submenu-list"> <li> <a href="/web/20230208102344/https://www.nist.gov/director" class="usa-nav__link">Office of the Director</a> </li> <li> <a href="/web/20230208102344/https://www.nist.gov/director/nist-organization-structure/budget-planning" class="usa-nav__link">Budget &amp; Planning</a> </li> </ul> </li> </ul></div><div class="column-break tablet:grid-col-fill"><ul class="usa-nav__submenu-list"> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/about-nist/work-nist" class="usa-nav__link">Work with NIST</a> </li> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/history" class="usa-nav__link">History</a> <ul class="usa-nav__submenu-list"> <li> <a href="https://web.archive.org/web/20230208102344/http://nistdigitalarchives.contentdm.oclc.org/" class="usa-nav__link">NIST Digital Archives</a> </li> <li> <a href="/web/20230208102344/https://www.nist.gov/nist-museum" class="usa-nav__link">NIST Museum</a> </li> <li> <a href="/web/20230208102344/https://www.nist.gov/nist-and-nobel" class="usa-nav__link">NIST and the Nobel</a> </li> </ul> </li> <li class="usa-nav__submenu-item"> <a href="/web/20230208102344/https://www.nist.gov/education" class="usa-nav__link">Educational Resources</a> </li> </ul> </div> </div> </div> </li> </ul> </div> </nav> </header> <!-- nist-index-ignore-end --> </div> <div class="grid-container"> <div data-drupal-messages-fallback class="hidden"></div> </div> <section class="nist-page__content usa-section clearfix"> <a id="main-content" tabindex="-1"></a> <div class="grid-container margin-top-4"> <div class="grid-row grid-gap-6"> <div class="nist-page__region nist-page__region--content tablet-lg:grid-col-12"> <div id="block-nist-www-content" class="nist-block"> <section class="nist-page__content usa-section clearfix"> <a id="main-content" tabindex="-1"></a> <div class="grid-container margin-top-4"> <div class="nist-page__region nist-page__region--content-top"> <div class="nist-block"> <a class="usa-button usa-button--accent-cool" href="/web/20230208102344/https://www.nist.gov/image-gallery">IMAGES</a> </div> <div class="nist-block"> <!-- nist-index-ignore-start --> <div data-elastic-exclude class="nist-block nist-social__wrapper"> <h2 class="nist-social__title">Share</h2> <div class="social-media-sharing"> <div class="nist-social__wrapper--share"> <div> <a target="_blank" rel="noopener" href="https://web.archive.org/web/20230208102344/https://www.facebook.com/share.php?u=https://www.nist.gov/image/moleculespng" title="Facebook" class="nist-social nist-social--facebook-gray"> <span>Facebook</span> </a> </div> <div> <a target="_blank" rel="noopener" href="https://web.archive.org/web/20230208102344/https://www.linkedin.com/shareArticle?mini=true&amp;url=https://www.nist.gov/image/moleculespng&amp;source=https://www.nist.gov/image/moleculespng" title="Linkedin" class="nist-social nist-social--linkedin-gray"> <span>Linkedin</span> </a> </div> <div> <a target="_blank" rel="noopener" href="https://web.archive.org/web/20230208102344/https://twitter.com/intent/tweet?url=https://www.nist.gov/image/moleculespng&amp;status=https://www.nist.gov/image/moleculespng" title="Twitter" class="nist-social nist-social--twitter-gray"> <span>Twitter</span> </a> </div> <div> <a href="https://web.archive.org/web/20230208102344/mailto:/?subject=NIST.gov&amp;body=Check out this site https://www.nist.gov/image/moleculespng" title="Email" class="nist-social nist-social--envelope-gray"> <span>Email</span> </a> </div> </div> </div> </div> <!-- nist-index-ignore-end --> </div> <div class="nist-block"> <h1 class="nist-page__title">individual fluorescent molecules</h1> </div> </div> </div> <div class="grid-container margin-top-4"> <div class="grid-row grid-gap-6"> <div class="nist-page__region nist-page__region--content tablet-lg:grid-col-8"> <div class="nist-block"> <div class="image"> <a href="https://web.archive.org/web/20230208102344/https://www.nist.gov/sites/default/files/images/2019/02/25/molecules.png"><img src="/web/20230208102344im_/https://www.nist.gov/sites/default/files/styles/960_x_960_limit/public/images/2019/02/25/molecules.png?itok=vi_uWZPa" width="960" height="960" alt="individual fluorescent molecules" loading="lazy" typeof="foaf:Image"/> </a> </div> </div> <div class="nist-block"> <h2 class="nist-block__title">Appears In</h2> <ul><li><a href="/web/20230208102344/https://www.nist.gov/news-events/news/2019/02/avoiding-crack-doom">Avoiding the Crack of Doom</a></li><li><a href="/web/20230208102344/https://www.nist.gov/programs-projects/super-resolution-optical-microscopy">Super-resolution optical microscopy</a></li></ul> </div> </div> <aside class="nist-page__region nist-page__region--sidebar-second tablet-lg:grid-col-4"> <div> <div class="nist-block"> <p>In this image, 200 nanometers (nm, billionths of a meter) square image, the size and direction of the arrows show the average local alignment of individual fluorescent molecules as a result of the strain produced by a square punch (the size of the gray dotted line) driven into the material, and then pulled out at an angle towards the top of the image.聽</p> </div> <div class="nist-block"> <a class="usa-button usa-button--accent-cool" href="/web/20230208102344/https://www.nist.gov/sites/default/files/images/2019/02/25/molecules.png" download>Download full image</a> </div> <div class="nist-block nist-block--no-margin"> <div class="nist-field nist-field--label-inline string"> <div class="nist-field__label">Credit</div> <div class="nist-field__item">NIST</div> </div> </div> <div class="nist-block nist-block--no-margin"> <div class="nist-field nist-field--label-inline nist-field--link-list entity-reference"> <div class="nist-field__label">Image Type</div> <div class="nist-field__items"> <div class="nist-field__item">Illustration</div> </div> </div> </div> </div> </aside> </div> </div> <div class="grid-container"> </div> </section> </div> </div> </div> </div> <div class="grid-container"> </div> </section> <div data-elastic-exclude> <!-- nist-index-ignore-start --> <footer class="nist-footer padding-bottom-4"> <div class="grid-container nist-footer__info"> <div class="grid-row"> <div class="tablet:grid-col-6"> <div class="nist-footer__logo"> <a href="/web/20230208102344/https://www.nist.gov/" title="National Institute of Standards and Technology" rel="home"> <img class="nist-footer__logo-img" src="/web/20230208102344im_/https://www.nist.gov/libraries/nist-component-library/dist/img/logo/NIST-Logo-Brand-White.svg" alt="National Institute of Standards and Technology logo" width="300px" height="42px"/> </a> </div> <div class="nist-footer__contact"> <h3 class="nist-footer__contact-heading">HEADQUARTERS</h3> <address> 100 Bureau Drive<br> Gaithersburg, MD 20899<br> <a href="https://web.archive.org/web/20230208102344/tel:301-975-2000">301-975-2000</a> </address> <p> <a href="https://web.archive.org/web/20230208102344/mailto:do-webmaster@nist.gov">Webmaster</a> | <a href="https://web.archive.org/web/20230208102344/https://www.nist.gov/about-nist/contact-us">Contact Us</a> | <a href="https://web.archive.org/web/20230208102344/https://www.nist.gov/visit">Our Other Offices</a> </p> </div> </div> <div class="tablet:grid-col-6"> <div class="nist-footer__social-links"> <a class="nist-social nist-social--twitter-white" href="https://web.archive.org/web/20230208102344/https://twitter.com/NIST"> <span>Twitter</span> </a> <a class="nist-social nist-social--facebook-white" href="https://web.archive.org/web/20230208102344/https://www.facebook.com/NIST"> <span>Facebook</span> </a> <a class="nist-social nist-social--linkedin-white" href="https://web.archive.org/web/20230208102344/https://www.linkedin.com/company/nist"> <span>LinkedIn</span> </a> <a class="nist-social nist-social--instagram-white" href="https://web.archive.org/web/20230208102344/https://www.instagram.com/nist/"> <span>Instagram</span> </a> <a class="nist-social nist-social--youtube-white" href="https://web.archive.org/web/20230208102344/https://www.youtube.com/NIST"> <span>YouTube</span> </a> <a class="nist-social nist-social--giphy-white" href="https://web.archive.org/web/20230208102344/https://giphy.com/nist"> <span>Giphy</span> </a> <a class="nist-social nist-social--rss-white" href="https://web.archive.org/web/20230208102344/https://www.nist.gov/news-events/nist-rss-feeds"> <span>RSS Feed</span> </a> <a class="nist-social nist-social--envelope-white" href="https://web.archive.org/web/20230208102344/https://public.govdelivery.com/accounts/USNIST/subscriber/new"> <span>Mailing List</span> </a> </div> <div class="nist-footer__feedback"> How are we doing? <a class="usa-button" rel="nofollow" href="/web/20230208102344/https://www.nist.gov/form/nist-gov-feedback?destination=/image/moleculespng" title="Provide feedback">Feedback</a> </div> </div> </div> </div> <div class="grid-container"> <div class="nist-footer__nav" role="navigation"> <ul> <li class="nist-footer__menu-item"> <a href="https://web.archive.org/web/20230208102344/https://www.nist.gov/privacy-policy">Site Privacy</a> </li> <li class="nist-footer__menu-item"> <a href="https://web.archive.org/web/20230208102344/https://www.nist.gov/oism/accessibility">Accessibility</a> </li> <li class="nist-footer__menu-item"> <a href="https://web.archive.org/web/20230208102344/https://www.nist.gov/privacy">Privacy Program</a> </li> <li class="nist-footer__menu-item"> <a href="https://web.archive.org/web/20230208102344/https://www.nist.gov/oism/copyrights">Copyrights</a> </li> <li class="nist-footer__menu-item"> <a href="https://web.archive.org/web/20230208102344/https://www.commerce.gov/vulnerability-disclosure-policy">Vulnerability Disclosure</a> </li> <li class="nist-footer__menu-item"> <a href="https://web.archive.org/web/20230208102344/https://www.nist.gov/no-fear-act-policy">No Fear Act Policy</a> </li> <li class="nist-footer__menu-item"> <a href="https://web.archive.org/web/20230208102344/https://www.nist.gov/office-director/freedom-information-act">FOIA</a> </li> <li class="nist-footer__menu-item"> <a href="https://web.archive.org/web/20230208102344/https://www.nist.gov/environmental-policy-statement">Environmental Policy</a> </li> <li class="nist-footer__menu-item"> <a href="https://web.archive.org/web/20230208102344/https://www.nist.gov/summary-report-scientific-integrity">Scientific Integrity</a> </li> <li class="nist-footer__menu-item"> <a href="https://web.archive.org/web/20230208102344/https://www.nist.gov/nist-information-quality-standards">Information Quality Standards</a> </li> <li class="nist-footer__menu-item"> <a href="https://web.archive.org/web/20230208102344/https://www.commerce.gov/">Commerce.gov</a> </li> <li class="nist-footer__menu-item"> <a href="https://web.archive.org/web/20230208102344/http://www.science.gov/">Science.gov</a> </li> <li class="nist-footer__menu-item"> <a href="https://web.archive.org/web/20230208102344/http://www.usa.gov/">USA.gov</a> </li> <li class="nist-footer__menu-item"> <a href="https://web.archive.org/web/20230208102344/https://vote.gov/">Vote.gov</a> </li> </ul> </div> </div> </footer> <!-- nist-index-ignore-end --> </div> </div> <script type="application/json" data-drupal-selector="drupal-settings-json">{"path":{"baseUrl":"\/","scriptPath":null,"pathPrefix":"","currentPath":"media\/485396","currentPathIsAdmin":false,"isFront":false,"currentLanguage":"en"},"pluralDelimiter":"\u0003","suppressDeprecationErrors":true,"ajaxPageState":{"theme":"nist_www","libraries":"back_to_top\/back_to_top_icon,back_to_top\/back_to_top_js,extlink\/drupal.extlink,figcaption_filter\/figcaption,google_analytics\/google_analytics,nist\/global,nist\/global-js,nist\/header-js,nist_linkit_filter\/nist_linkit_filter_ga,nist_www\/global,nist_www\/global-js,social_media\/basic,system\/base"},"back_to_top":{"back_to_top_button_trigger":100,"back_to_top_prevent_on_mobile":false,"back_to_top_prevent_in_admin":true,"back_to_top_button_type":"image","back_to_top_button_text":"Back to top"},"google_analytics":{"account":"UA-66610693-3","trackOutbound":true,"trackMailto":true,"trackTel":true,"trackDownload":true,"trackDownloadExtensions":"7z|aac|arc|arj|asf|asx|avi|bin|bsh|c|csv|doc(x|m)?|dot(x|m)?|dw(fx|g|gd)|dxf|eps|epub|exe|f(90)|flv|gif|gz|gzip|hqx|jar|jpe?g|js|m1v|mp(2|3|4|e?g)|mobi|mov(ie)?|msi|msp|pdf|phps|pl|png|ppt(x|m)?|pot(x|m)?|pps(x|m)?|ppam|sld(x|m)?|thmx|qtm?|ra(m|r)?|rfa|rtf|rvt|sch|sea|sit|swf|tar|tgz|tif|txt|txz|wav|wma|wmv|wpd|wrl|xls(x|m|b)?|xlt(x|m)|xlam|xml|xsd|z|zip","trackDomainMode":1},"nist_search":{"clickTracking":"search-report-click","isDebug":false,"clickTrackEnabled":true,"message":"NIST Search in debug mode. Check the browsers network inspector for Click Track reporting results..."},"data":{"extlink":{"extTarget":false,"extTargetNoOverride":false,"extNofollow":false,"extNoreferrer":false,"extFollowNoOverride":false,"extClass":"ext","extLabel":"(link is external)","extImgClass":false,"extSubdomains":true,"extExclude":"\\.gov\\\/|\\.mil\\\/|\\manufacturingusa\\.com\\\/","extInclude":"","extCssExclude":"","extCssExplicit":"","extAlert":true,"extAlertText":"Thank you for visiting NIST. We hope your visit was informative. We have provided a link to this site because it has information that may be of interest to our users. NIST does not necessarily endorse the views expressed or the facts presented on this site. Further, NIST does not endorse any commercial products that may be advertised or available on this site. Click OK to be directed to your link.","mailtoClass":"0","mailtoLabel":"(link sends email)","extUseFontAwesome":false,"extIconPlacement":"append","extFaLinkClasses":"fa fa-external-link","extFaMailtoClasses":"fa fa-envelope-o","whitelistedDomains":[]}},"user":{"uid":0,"permissionsHash":"582eea317b4636ab5a0dffc8c6a006df3d6901ea5610498a0bbefc925ea1da0f"}}</script> <script src="/web/20230208102344js_/https://www.nist.gov/sites/default/files/js/js_DNL6VrEuHPrL959oUExCzGY3A7BXQIMYib7aZLaICi4.js"></script> <script src="https://web.archive.org/web/20230208102344js_/https://siteimproveanalytics.com/js/siteanalyze_6017546.js" async></script> <script src="/web/20230208102344js_/https://www.nist.gov/sites/default/files/js/js_XoevpgMqpfZ_vQSIDKbsBW_ECleGCiKLbn2hnxI0QMg.js"></script> <script type="text/javascript">window.NREUM||(NREUM={});NREUM.info={"beacon":"gov-bam.nr-data.net","licenseKey":"37b7ccb661","applicationID":"1089704227","transactionName":"YFxUN0sADEdYVkBaClkWdwBNCA1aFkVVVABoWlcAUQQ=","queueTime":0,"applicationTime":46,"atts":"TBtXQQMaH0k=","errorBeacon":"gov-bam.nr-data.net","agent":""}</script></body> </html> <!-- FILE ARCHIVED ON 10:23:44 Feb 08, 2023 AND RETRIEVED FROM THE INTERNET ARCHIVE ON 03:41:52 Dec 02, 2024. JAVASCRIPT APPENDED BY WAYBACK MACHINE, COPYRIGHT INTERNET ARCHIVE. ALL OTHER CONTENT MAY ALSO BE PROTECTED BY COPYRIGHT (17 U.S.C. SECTION 108(a)(3)). --> <!-- playback timings (ms): captures_list: 0.562 exclusion.robots: 0.026 exclusion.robots.policy: 0.016 esindex: 0.01 cdx.remote: 9.625 LoadShardBlock: 303.855 (3) PetaboxLoader3.datanode: 92.115 (4) PetaboxLoader3.resolve: 177.424 (3) load_resource: 118.559 -->

Pages: 1 2 3 4 5 6 7 8 9 10