{"title":"Determination of Alkaline Protease Production In Serratia Marcescens Sp7 Using Agro Wastes As Substrate Medium, Optimization Of Production Parameters And Purification Of The Enzyme","authors":"Baby Joseph, Sankarganesh Palaniyandi","volume":50,"journal":"International Journal of Biotechnology and Bioengineering","pagesStart":61,"pagesEnd":66,"ISSN":"1307-6892","URL":"https:\/\/publications.waset.org\/pdf\/8153","abstract":"The enzyme alkaline protease production was determined under\r\nsolid state fermentation using the soil bacteria Serratia marcescens\r\nsp7. The maximum production was obtained from wheat bran\r\nmedium than ground nut shell and chemically defined medium. The\r\nphysiological fermentation factors such as pH of the medium (pH 8),\r\nTemperature (40oC) and incubation time (48 hrs) played a vital role\r\nin alkaline protease production in all the above. 100Mm NaCl has\r\ngiven better resolution during elution of the enzymes. The enzyme\r\nproduction was found to be associated with growth of the bacterial\r\nculture.","references":"[1] R. Gupta, Q. K. Beg, S. Khan, and B. Chauhan, \"An overview on\r\nfermentation, downstream processing and properties of microbial alkaline\r\nproteases,\", Appl. Microbiol. Biotech. 60, 381-395, 2002a\r\n[2] R. Gupta, Q.K. Beg, P. Lorenz, (2002b), \"Bacterial Alkaline\r\nProtease: Molecular approaches and industrial application,\" Appl. Microbial.\r\nBiotechnol., 59, 15-32, 2002b.\r\n[3] Francisco, J. Ust\u251c\u00edriz, L. Adriana, A. G. Luis, A.G, Mario, D,\r\n\"Fermentation conditions increasing protease production by Serratia\r\nmarcescens in fresh whey,\" Rev. T\u00e9c. Ing. Univ. Zulia. 31(1), 79-89, 2008.\r\n[4] M. Kalpana Devi, B.A. Rasheedha, G.R. Gnanaprabhal,\r\nB.V.Pradeep, M. Palaniswamy, \"Purification, characterization of alkaline\r\nprotease enzyme from native isolate Aspergillus niger and its compatibility\r\nwith commercial detergents,\" Ind. J. Science. Tech. 1(7), 1-6, 2008\r\n[5] Karl-Heinz Maurer, \"Detergent proteases,\" Curr. Opi. Biotech. 15,\r\n330-334, 2004.\r\n[6] D.P.N. Ramakrishna, N. Gopi Reddy, S.V. Rajagopal S.V,\r\n\"Purification and Properties of an Extra Cellular Alkaline Protease Produced\r\nBy Bacillus Subtilis (MTTC N0-10110)\". Internat. J. Biotech. Biochem.\r\n6(4), 493-504, 2010.\r\n[7] Hamid mukhtar, Ikram-ul-haq, \"Production of alkaline protease by\r\nBacillus subtilis and its application as a depilating agent in leather\r\nprocessing,\" Pak. J. Bot. 40(4), 1673-1679, 2008.\r\n[8] Vishalakshi, N.; Lingappa, K.; Amena, S.; Prabhakar, M.;\r\nDayanand. (2009). Production of alkaline protease from Streptomyces\r\ngulbargensis and its application in removal of blood strains. Ind. J. Biotech. 8,\r\n280-285.\r\n[9] A. Sumantha, C. Larroche, P. Ashok, \"Microbiology and Industrial\r\nBiotechnology of Food-Grade Proteases: A Perspective,\" Food Technol.\r\nBiotechnol. 44 (2), 211-220, 2006.\r\n[10] J. R. Simkhada, M. Poonam, S.C. Seung, C.Y. Jin, \"A novel\r\nfibrinolytic protease from Streptomyces sp. CS684\" Process Biochem. 45, 88-\r\n93, 2010.\r\n[11] P. Bjorn, Johansson, S. Oonagh, B. Lars, IdeS: A Bacterial\r\nProteolytic Enzyme with Therapeutic Potential. PLoS ONE 3(2), e1692.\r\ndoi:10.1371\/ journal.pone.0001692, 2008.\r\n[12] R. Selvakumar, Sathis kumar, K. Swaminathan, \"Purification and\r\nwash performance analysis of thermostable extracellular alkaline protease\r\nproduced by soil bacterium bacillus sp.GOS-2\". Asia. J. Microbiol. Biotech.\r\nEnv. Sci. 10, 29-35, 2008.\r\n[13] O.H. Lowry, N. Rosebrough, A. L. Farr, R.L. Ronadall, \" Protein\r\nmeasurement with the folin phenol reagent,\" J. Biol. chem. 193, 265-273,\r\n1951.\r\n[14] R.S.Prakasham, R.C. Subba, P.N. Sharma, Green gram husk\u00d4\u00c7\u00f6an\r\ninexpensive substrate for alkaline protease production by Bacillus sp. in solidstate\r\nfermentation,\" Bioresour. Tech. 97, 1449-1454, 2006.\r\n[15] R. Paranthaman, K. Alagusundaram, J. Indhumathi, \"Production of\r\nProtease from Rice Mill Wastes by Aspergillus niger in Solid State\r\nFermentation,\" Worl. J. Agri. Sciences. 5 (3), 308-312, 2009.\r\n[16] N. Muhammad, I. Q. Javed, B. Shahjahan, S. Qurat-ul-Ain, Effect\r\nof Medium Composition on Commercially Important Alkaline Protease\r\nProduction by Bacillus licheniformis N-2\". Food Technol. Biotechnol. 46 (4),\r\n388-394, 2008.","publisher":"World Academy of Science, Engineering and Technology","index":"Open Science Index 50, 2011"}