Proteome Profiler Antibody Arrays Rapid Protein Profiling

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</div> </div> <main> <section class="section"> <div> <div id="block-bio-techne-global-content" class="system_main_block"> <article> <div> <div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a href="#search">Search Proteome Profiler Antibody Arrays</a>  | <a href="#quickspots">Quick Spots Image Analysis Software</a> | <a href="#antibody-array">Antibody Array Principle</a> | <a href="#resources">Additional Resources</a></p></div> </div> </div> <div> <div class="paragraph accordion mb-4 paragraph--type--extendable-paragraph paragraph--view-mode--default"> <div class="card-header rounded-top bg-white"> <a class="collapsed" data-toggle="collapse" href="#expPara-what-are-proteome-profiler-antibody-arrays2" role="button" aria-expanded="false" aria-controls="expPara"> <h5>What Are Proteome Profiler Antibody Arrays?</h5> </a> </div> <div class="collapse" id="expPara-what-are-proteome-profiler-antibody-arrays2" aria-expanded="false" aria-controls="expPara"> <div class="card-body p-4"> <br/> <div><h4>Explore Proteome Profiler™ Antibody Arrays</h4> <p>Discover the innovation of Proteome Profiler Antibody Arrays, cost-effective tools designed for early-stage research exploration with significant differentiating features.</p> <p>This antibody array system, offering semiquantitative analysis, empowers researchers to measure over 100 proteins in a single sample. The simplicity of this method is underscored by its independence from specialized equipment, rendering multiple Western blot experiments unnecessary. Each antibody array kit comprises buffers, detection antibodies, and membranes meticulously spotted in duplicate with high-quality capture antibodies. Utilizing chemiluminescence for detection, the resulting data can be quantified with the same precision as traditional Western blots.</p> <p>Select antibody arrays are also suitable for use with the LI-COR® detection systems.</p> <p>Ready for a more quantitative multiplex assay? Try our <a href="/reagents/luminex-assays">Luminex® Assays</a>.</p></div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="search"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <h2>Search Proteome Profiler Antibody Arrays</h2> <div class="row card_row"> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/search?keywords=proteome%20profiler&species=Human" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: #f5f5f5; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/proteome-human-blot.png" alt="The Proteome Profiler Antibody Array measures the effect of competitors on human protein expression." loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/search?keywords=proteome%20profiler&species=Human" > <h3 class="card_title_htag"> <div><h4>Profile Human Extracellular or Intracellular Proteins</h4></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Use the Proteome Profiler antibody array to screen human proteins such as <a href="/search?keywords=cytokine&category_attr=Proteome%20Profiler%20Antibody%20Arrays&species=Human">cytokines</a>, <a href="/search?keywords=cancer&category_attr=Proteome%20Profiler%20Antibody%20Arrays&species=Human">cancer biomarkers</a>, <a href="/search?keywords=kinase&category_attr=Proteome%20Profiler%20Antibody%20Arrays&species=Human">kinases</a>, <a href="/search?keywords=soluble%20receptors&category_attr=Proteome%20Profiler%20Antibody%20Arrays&species=Human">soluble receptors</a>, <a href="/search?keywords=ubiquitin&category_attr=Proteome%20Profiler%20Antibody%20Arrays">ubiquitin-related proteins</a> <a href="/search?keywords=protease&category_attr=Proteome%20Profiler%20Antibody%20Arrays&species=Human">proteases</a>, <a href="/search?keywords=protease%20inhibitor%20array%20kits&category_attr=Proteome%20Profiler%20Antibody%20Arrays&species=Human">protease inhibitors</a> and more!</p></div> </div> <div class="card-bottom-link"> <div> <a href="/search?keywords=proteome%20profiler&species=Human" > Profile Human Proteins </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/search?keywords=proteome%20profiler&species=Mouse" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: #f5f5f5; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/proteome-mouse-blot.png" alt="2. Use the Proteome Profiler Antibody Array to measure LPS stimulated changes in mouse intracellular or extracellular proteins." loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/search?keywords=proteome%20profiler&species=Mouse" > <h3 class="card_title_htag"> <div><h4>Profile Mouse Extracellular or Intracellular Proteins</h4></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Use Proteome Profiler antibody arrays to screen mouse proteins such as <a href="/t/adipokine-array-kit/antibody-arrays?species=Mouse">adipokines</a>, <a href="/search?keywords=cytokine&category_attr=Proteome%20Profiler%20Antibody%20Arrays&species=Mouse">cytokines</a>, <a href="/search?keywords=chemokine&category_attr=Proteome%20Profiler%20Antibody%20Arrays&species=Mouse">chemokines</a>,<a href="/search?keywords=chemokine&category_attr=Proteome%20Profiler%20Antibody%20Arrays&species=Mouse"> kinases</a>, <a href="/t/apoptosis-array-kit/antibody-arrays?species=Mouse">apoptotic proteins</a> and <a href="/t/angiogenesis-assay-kits?category_attr=Proteome%20Profiler%20Antibody%20Arrays">angiogenesis</a> related proteins.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/search?keywords=proteome%20profiler&species=Mouse" > Profile Mouse Proteins </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/search?keywords=proteome%20profiler&species=Rat" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: #f5f5f5; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/proteome-rat-blot.png" alt="Use the Proteome Profiler Antibody Array to measure lipopolysaccharide (LPS) stimulated changes in rat adipokines or cytokines." loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/search?keywords=proteome%20profiler&species=Rat" > <h3 class="card_title_htag"> <div><h4>Profile Rat Adipokines and Cytokines</h4></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Profile rat <a href="/t/adipokine-array-kit/antibody-arrays?species=Rat">adipokines</a> and <a href="/search?keywords=cytokine&category_attr=Proteome%20Profiler%20Antibody%20Arrays&species=Rat">cytokines</a> with Proteome Profiler antibody arrays.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/search?keywords=proteome%20profiler&species=Rat" > Profile Rat Proteins </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="quickspots"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--_-1-text-to-media mb-4 paragraph--view-mode--default p-3"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--75-25"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:3_1_text_to_media:field_header_2_global"> <div><h2>Quick Spots Image Analysis Software</h2></div> </div> <div class="field_block:paragraph:3_1_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p>R&D Systems<sup>®</sup> now offers Quick Spots image analysis software designed specifically for your Proteome Profiler<sup>™</sup> Arrays.</p> <p>No more laborious set-up of templates in other imaging software, Quick Spots contains preloaded templates for each Proteome Profiler<sup>™</sup> array. It knows where your results should be and provides the data in less time and with less work.</p></div> </div> <div class="field_block:paragraph:3_1_text_to_media:field_call_to_action_global"> <a href="https://idealeyes.com/products/QuickSpots.php" class="btn btn-primary" type="link"> Download Quick Spots </a> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:3_1_text_to_media:field_indv_media"> <div> <a href="https://idealeyes.com/products/QuickSpots.php" title="Download Quick Spots" target="_blank" rel="noreferrer" > <img src="https://resources.bio-techne.com/bio-techne-assets/images/protocols/v2_QuickSpots2-hero-608.png" alt="Quick Spots Image Analysis Software" loading="lazy" > </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <div class="row card_row"> <div class="col-md-6"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="https://www.youtube.com/watch?v=R0ETosGw5Y4&list=PL8ieOvGKu8fo4c_rI_arwq28c-NlYLEJE&index=30" > <div class="teaser-card-image-crop mb-3"> <img loading="lazy" class="card-background-image card-img-top" src="https://resources.bio-techne.com/bio-techne-assets/images/protocols/v2_proteome-profiler_teaser-bg-1.png" /> </div> <div class="card-foreground-image"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/icons/webinar-icon-new-white.png" alt="Webinar Icon" loading="lazy" > </div> </a> <a class="card_title_link" href="https://www.youtube.com/watch?v=R0ETosGw5Y4&list=PL8ieOvGKu8fo4c_rI_arwq28c-NlYLEJE&index=30" > <h3 class="card_title_htag"> <div>Webinar: Understanding Immunosuppressive Myeloid Cells and Immunotherapy</div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Advanced Prostate Cancer (PCa) can progress to lethal metastatic castration-resistant prostate cancer (mCRPC), even when treated with androgen deprivation therapy.  Although immune checkpoint blockade has revolutionized the way cancer is treated, it is less effective in this context. Watch this video to see how Proteome Profiler antibody arrays were used by Dr. Xin Lu and his research team as they addressed this challenge. See how they demonstrated increased synergistic antitumor efficacy when an immune checkpoint blockade cocktail (CTLA-4 and PD1) is used in combination with PI3-kinase signaling pathway inhibitors. Find out about the role that myeloid-derived suppressor cells play in aggressive cancer.</p></div> </div> <div class="card-bottom-link"> <div> <a href="https://www.youtube.com/watch?v=R0ETosGw5Y4&list=PL8ieOvGKu8fo4c_rI_arwq28c-NlYLEJE&index=30" > Watch Dr Xin Lu's Webinar </a> </div> </div> </div> </div> </div> <div class="col-md-6"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="https://youtu.be/1lt1f_-EIwg" > <div class="teaser-card-image-crop mb-3"> <img loading="lazy" class="card-background-image card-img-top" src="https://resources.bio-techne.com/bio-techne-assets/images/protocols/v2_proteome-profiler_teaser-bg-2.png" /> </div> <div class="card-foreground-image"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/icons/webinar-icon-new-white.png" alt="Webinar Icon" loading="lazy" > </div> </a> <a class="card_title_link" href="https://youtu.be/1lt1f_-EIwg" > <h3 class="card_title_htag"> <div><h3>Profiling Changes in Receptor Tyrosine Kinase Phosphorylation Using Antibody</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Watch this video to learn about the principle of the Proteome Profiler antibody array. See how assays are run with this step-by-step detailed description of the method.</p></div> </div> <div class="card-bottom-link"> <div> <a href="https://youtu.be/1lt1f_-EIwg" > Learn How to Use Proteome Profiler </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="antibody-array"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-media-to-text mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/protocols/proteome-profiler-antibody-array.png" alt="Proteome Profiler Antibody Array" loading="lazy" > </div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_header_2_global"> <div><h2>Antibody Array Principle</h2></div> </div> <div class="field_block:paragraph:2_2_media_to_text:field_lg_sngl_frmtd_text_global"> <div><p> </p> <ol> <li>Capture antibodies are spotted in duplicate on the nitrocellulose membrane.</li> <li>Spotted membrane arrays are incubated in a single lysate.</li> <li>Target analytes are captured by the capture antibody.</li> <li>Target analytes are detected using a biotinylated or horseradish peroxidase (HRP)-conjugated detection antibody.</li> <li>Biotinylated detection antibodies are reacted with streptavidin HRP.</li> <li>Membranes are incubated in a chemiluminescence reagent that produces a light signal proportional to the amount of antibody-target analyte complexes formed.</li> <li>Data is visualized using standard western blotting methods such as film or digital 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