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class="u-taCenter"></div><div class="profile--tab_content_container js-tab-pane tab-pane active" id="all"><div class="profile--tab_heading_container js-section-heading" data-section="Papers" id="Papers"><h3 class="profile--tab_heading_container">Papers by Dawidson Gomes</h3></div><div class="js-work-strip profile--work_container" data-work-id="18923915"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/18923915/Suppression_of_Nuclear_and_Cytosolic_Calcium_Signaling_by_Targeted_Expression_of_Calretinin"><img alt="Research paper thumbnail of Suppression of Nuclear and Cytosolic Calcium Signaling by Targeted Expression of Calretinin" class="work-thumbnail" src="https://attachments.academia-assets.com/42150432/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link 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class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/18923914/Characterization_of_Decellularized_Heart_Matrices_as_Biomaterials_for_Regular_and_Whole_Organ_Tissue_Engineering_and_Initial_In_vitro_Recellularization_with_Ips_Cells">Characterization of Decellularized Heart Matrices as Biomaterials for Regular and Whole Organ Tissue Engineering and Initial In-vitro Recellularization with Ips Cells</a></div><div class="wp-workCard_item"><span>Journal of tissue science & engineering</span><span>, Jan 28, 2012</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Tissue engineering strategies, based on solid/porous scaffolds, suffer from several limitations, ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Tissue engineering strategies, based on solid/porous scaffolds, suffer from several limitations, such as ineffective vascularization, poor cell distribution and organization within scaffold, in addition to low final cell density, among others. Therefore, the search for other tissue engineering approaches constitutes an active area of investigation. Decellularized matrices (DM) present major advantages compared to solid scaffolds, such as ideal chemical composition, the preservation of vascularization structure and perfect three-dimensional structure. In the present study, we aimed to characterize and investigate murine heart decellularized matrices as biomaterials for regular and whole organ tissue engineering. Heart decellularized matrices were characterized according to: 1. DNA content, through DNA quantificationo and PCR of isolated genomic DNA; 2. Histological structure, assessed after Hematoxylin and Eosin, as well as Masson&#39;s Trichrome stainings; 3. Surface nanostructure a...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="18923914"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="18923914"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 18923914; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=18923914]").text(description); $(".js-view-count[data-work-id=18923914]").attr('title', description).tooltip(); 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="18923913"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/18923913/IFN_%CE%B3_Dependent_Recruitment_of_CD4_T_Cells_and_Macrophages_Contributes_to_Pathogenesis_During_Leishmania_amazonensis_Infection"><img alt="Research paper thumbnail of IFN-γ-Dependent Recruitment of CD4(+) T Cells and Macrophages Contributes to Pathogenesis During Leishmania amazonensis Infection" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/18923913/IFN_%CE%B3_Dependent_Recruitment_of_CD4_T_Cells_and_Macrophages_Contributes_to_Pathogenesis_During_Leishmania_amazonensis_Infection">IFN-γ-Dependent Recruitment of CD4(+) T Cells and Macrophages Contributes to Pathogenesis During Leishmania amazonensis Infection</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://ufmg.academia.edu/DawidsonGomes">Dawidson Gomes</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://ufmg.academia.edu/MateusEust%C3%A1quioMouraLopes">Mateus Eustáquio Moura Lopes</a></span></div><div class="wp-workCard_item"><span>Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research</span><span>, Jan 24, 2015</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Interferon gamma (IFN-γ) is a key factor in the protection of hosts against intracellular parasit...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Interferon gamma (IFN-γ) is a key factor in the protection of hosts against intracellular parasites. This cytokine induces parasite killing through nitric oxide and reactive oxygen species production by phagocytes. Surprisingly, during Leishmania amazonensis infection, IFN-γ plays controversial roles. During in vitro infections, IFN-γ induces the proliferation of the amastigote forms of L. amazonensis. However, this cytokine is not essential at the beginning of an in vivo infection. It is not clear why IFN-γ does not mediate protection during the early stages of infection. Thus, the aim of our study was to investigate the role of IFN-γ during L. amazonensis infection. We infected IFN-γ(-/-) mice in the footpad and followed the development of leishmaniasis in these mice compared with that in WT mice. CD4(+) T lymphocytes and macrophages migrated earlier to the site of infection in the WT mice, and the earlier migration of these 2 cell types was associated with lesion development and ...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="18923913"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="18923913"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 18923913; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=18923913]").text(description); $(".js-view-count[data-work-id=18923913]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 18923913; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='18923913']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); 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</script> <div class="js-work-strip profile--work_container" data-work-id="18923912"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/18923912/Verifica%C3%A7%C3%A3o_experimental_para_confirma%C3%A7%C3%A3o_de_t%C3%A9cnica_in_vitro_do_efeito_bystander_induzido_por_radia%C3%A7%C3%A3o_gama_na_linhagem_celular_CHO_K1"><img alt="Research paper thumbnail of Verificação experimental para confirmação de técnica in vitro do efeito bystander induzido por radiação gama na linhagem celular CHO-K1" class="work-thumbnail" src="https://attachments.academia-assets.com/40331578/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/18923912/Verifica%C3%A7%C3%A3o_experimental_para_confirma%C3%A7%C3%A3o_de_t%C3%A9cnica_in_vitro_do_efeito_bystander_induzido_por_radia%C3%A7%C3%A3o_gama_na_linhagem_celular_CHO_K1">Verificação experimental para confirmação de técnica in vitro do efeito bystander induzido por radiação gama na linhagem celular CHO-K1</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Verificação experimental para confirmação de técnica in vitro do efeito bystander induzido por ra...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Verificação experimental para confirmação de técnica in vitro do efeito bystander induzido por radiação gama na linhagem celular CHO-K1 O efeito bystander diz respeito a respostas biológicas detectadas em células não diretamente irradiadas, mas que são influenciadas, de alguma forma, por sinais transmitidos por células irradiadas vizinhas. Essas respostas biológicas incluem trocas entre cromátides irmãs, mutações, formação de micronúcleos, aberrações cromossômicas, carcinogêneses, além de morte por apoptose e necrose. Apesar de sua existência ser indiscutível, os mecanismos envolvidos no desencadeamento do efeito bystander ainda não foram completamente esclarecidos. Estudos anteriores comprovam que este efeito é dependente de uma grande variedade de parâmetros incluindo a dose, a taxa de dose, o tipo de radiação e tipo de célula ou tecido. O presente trabalho visa confirmar a técnica anteriormente utilizada na literatura em linhagens celulares humanas para a verificação do efeito by...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="aee2088c18554236c364ab107d59b6bc" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":40331578,"asset_id":18923912,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/40331578/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="18923912"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="18923912"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 18923912; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=18923912]").text(description); $(".js-view-count[data-work-id=18923912]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 18923912; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='18923912']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "aee2088c18554236c364ab107d59b6bc" } } $('.js-work-strip[data-work-id=18923912]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":18923912,"title":"Verificação experimental para confirmação de técnica in vitro do efeito bystander induzido por radiação gama na linhagem celular CHO-K1","internal_url":"https://www.academia.edu/18923912/Verifica%C3%A7%C3%A3o_experimental_para_confirma%C3%A7%C3%A3o_de_t%C3%A9cnica_in_vitro_do_efeito_bystander_induzido_por_radia%C3%A7%C3%A3o_gama_na_linhagem_celular_CHO_K1","owner_id":38951595,"coauthors_can_edit":true,"owner":{"id":38951595,"first_name":"Dawidson","middle_initials":null,"last_name":"Gomes","page_name":"DawidsonGomes","domain_name":"ufmg","created_at":"2015-11-23T03:22:12.839-08:00","display_name":"Dawidson Gomes","url":"https://ufmg.academia.edu/DawidsonGomes"},"attachments":[{"id":40331578,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/40331578/thumbnails/1.jpg","file_name":"Verificao_experimental_para_confirmao_de20151124-10454-fu0vx6.pdf","download_url":"https://www.academia.edu/attachments/40331578/download_file","bulk_download_file_name":"Verificacao_experimental_para_confirmaca.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/40331578/Verificao_experimental_para_confirmao_de20151124-10454-fu0vx6-libre.pdf?1448375341=\u0026response-content-disposition=attachment%3B+filename%3DVerificacao_experimental_para_confirmaca.pdf\u0026Expires=1739809940\u0026Signature=F3WyPjr3zV8WIzGdix-~-GL3GXlr7JFIviykuUeznJAAH0-mQkgoBak5pjubCRyglll6phRb57l07T4DWVnUDvtie013u5kcS-i9iy5yavxG-3p8SVk9XBmTHvrLiW7QVsBqJPj2EcBEQa8FsuE9fJfIKq4rnEW05c3NJQUCWwxyfJVLeUhKfyzsGGjmjO-tnbZIkgElH~-W8wOpvTc8mKKeECcu3~c1UJRd9oyY9~C-jVojHjxvmsTvbuuvBpgWdiUBdMHp40GE485viKY4KCw38dyNpN-gTeGTPDHSEXz~LHnjVmzI5sGoLJ5inNmqfZhM4hr9~eTAE0oPSwGzGg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="18923911"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/18923911/Protective_effect_of_green_tea_and_Arrabidea_chica_extracts_against_induced_doxorubicin_cardiotoxicity_in_vitro_without_interfering_on_antitumor_activity"><img alt="Research paper thumbnail of Protective effect of green tea and Arrabidea chica extracts against induced doxorubicin cardiotoxicity in vitro without interfering on antitumor activity" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/18923911/Protective_effect_of_green_tea_and_Arrabidea_chica_extracts_against_induced_doxorubicin_cardiotoxicity_in_vitro_without_interfering_on_antitumor_activity">Protective effect of green tea and Arrabidea chica extracts against induced doxorubicin cardiotoxicity in vitro without interfering on antitumor activity</a></div><div class="wp-workCard_item"><span>Toxicology Letters</span><span>, 2011</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="18923911"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="18923911"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 18923911; 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dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=18923911]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":18923911,"title":"Protective effect of green tea and Arrabidea chica extracts against induced doxorubicin cardiotoxicity in vitro without interfering on antitumor activity","internal_url":"https://www.academia.edu/18923911/Protective_effect_of_green_tea_and_Arrabidea_chica_extracts_against_induced_doxorubicin_cardiotoxicity_in_vitro_without_interfering_on_antitumor_activity","owner_id":38951595,"coauthors_can_edit":true,"owner":{"id":38951595,"first_name":"Dawidson","middle_initials":null,"last_name":"Gomes","page_name":"DawidsonGomes","domain_name":"ufmg","created_at":"2015-11-23T03:22:12.839-08:00","display_name":"Dawidson Gomes","url":"https://ufmg.academia.edu/DawidsonGomes"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="18923910"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/18923910/Production_of_Human_Endothelial_Cells_Free_from_Soluble_Xenogeneic_Antigens_for_Bioartificial_Small_Diameter_Vascular_Graft_Endothelization"><img alt="Research paper thumbnail of Production of Human Endothelial Cells Free from Soluble Xenogeneic Antigens for Bioartificial Small Diameter Vascular Graft Endothelization" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/18923910/Production_of_Human_Endothelial_Cells_Free_from_Soluble_Xenogeneic_Antigens_for_Bioartificial_Small_Diameter_Vascular_Graft_Endothelization">Production of Human Endothelial Cells Free from Soluble Xenogeneic Antigens for Bioartificial Small Diameter Vascular Graft Endothelization</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://ufmg.academia.edu/DawidsonGomes">Dawidson Gomes</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/Tha%C3%ADsMariaMataMartins">Thaís Maria Mata Martins</a></span></div><div class="wp-workCard_item"><span>BioMed Research International</span><span>, 2015</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Arterial bypass graft implantation remains the primary therapy for patients with advanced cardiov...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Arterial bypass graft implantation remains the primary therapy for patients with advanced cardiovascular disease, but most lack adequate saphenous vein or other conduits for bypass procedures and would benefit from a bioartificial conduit. This study aimed to produce human endothelial cells (hECs) in large scale, free from xenogeneic antigens, to develop a small diameter, compatible vessel for potential use as a vascular graft. Human adipose-derived stromal cells (hASCs) were isolated, cultured, and differentiated in the presence of human serum and used for the reendothelization of a decellularized rat aorta. hASC derived ECs (hASC-ECs) expressed VEGFR2, vWf and CD31 endothelial cell markers, the latter in higher levels than hASCs and HUVECs, and were shown to be functional. Decellularization protocol yielded aortas devoid of cell nuclei, with preserved structure, including a preserved basement membrane. When seeded with hASC-ECs, the decellularized aorta was completely reendothelized, and the hASC-ECs maintained their phenotype in this new condition. hASCs can be differentiated into functional hECs without the use of animal supplements and are capable of reendothelizing a decellularized rat aorta while maintaining their phenotype. The preservation of the basement membrane following decellularization supported the complete reendothelization of the scaffold with no cell migration towards other layers. This approach is potentially useful for rapid obtention of compatible, xenogeneic-free conduit.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="18923910"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="18923910"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 18923910; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=18923910]").text(description); $(".js-view-count[data-work-id=18923910]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 18923910; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='18923910']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=18923910]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":18923910,"title":"Production of Human Endothelial Cells Free from Soluble Xenogeneic Antigens for Bioartificial Small Diameter Vascular Graft Endothelization","internal_url":"https://www.academia.edu/18923910/Production_of_Human_Endothelial_Cells_Free_from_Soluble_Xenogeneic_Antigens_for_Bioartificial_Small_Diameter_Vascular_Graft_Endothelization","owner_id":38951595,"coauthors_can_edit":true,"owner":{"id":38951595,"first_name":"Dawidson","middle_initials":null,"last_name":"Gomes","page_name":"DawidsonGomes","domain_name":"ufmg","created_at":"2015-11-23T03:22:12.839-08:00","display_name":"Dawidson Gomes","url":"https://ufmg.academia.edu/DawidsonGomes"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="18923909"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/18923909/Nuclear_localization_of_epidermal_growth_factor_receptor_EGFR_in_ameloblastomas"><img alt="Research paper thumbnail of Nuclear localization of epidermal growth factor receptor (EGFR) in ameloblastomas" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/18923909/Nuclear_localization_of_epidermal_growth_factor_receptor_EGFR_in_ameloblastomas">Nuclear localization of epidermal growth factor receptor (EGFR) in ameloblastomas</a></div><div class="wp-workCard_item"><span>Oncotarget</span><span>, Jan 30, 2015</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Ameloblastoma is a locally invasive neoplasm often associated with morbidity and facial deformiti...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Ameloblastoma is a locally invasive neoplasm often associated with morbidity and facial deformities, showing increased Epidermal Growth Factor Receptor (EGFR) expression. Inhibition of EGFR was suggested as a treatment option for a subset of ameloblastomas. However, there are resistance mechanisms that impair anti-EGFR therapies. One important resistance mechanism for EGFR-inhibition is the EGFR nuclear localization, which activates genes responsible for its mitogenic effects, such as Cyclin D1. We assessed EGFR nuclear localization in encapsulated (unicystic, n = 3) and infiltrative (multicystic, n = 11) ameloblastomas and its colocalization with Cyclin D1 by using anti-EGFR and anti-lamin B1 double labeling immunofluorescence analyzed by confocal microscopy. Oral inflammatory fibrous hyperplasia and oral squamous cell carcinoma samples were used for comparison. Twelve cases of ameloblastoma exhibited nuclear EGFR colocalization with lamin B1. This positive staining was mainly obse...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="18923909"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="18923909"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 18923909; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=18923909]").text(description); $(".js-view-count[data-work-id=18923909]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 18923909; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='18923909']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=18923909]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":18923909,"title":"Nuclear localization of epidermal growth factor receptor (EGFR) in ameloblastomas","internal_url":"https://www.academia.edu/18923909/Nuclear_localization_of_epidermal_growth_factor_receptor_EGFR_in_ameloblastomas","owner_id":38951595,"coauthors_can_edit":true,"owner":{"id":38951595,"first_name":"Dawidson","middle_initials":null,"last_name":"Gomes","page_name":"DawidsonGomes","domain_name":"ufmg","created_at":"2015-11-23T03:22:12.839-08:00","display_name":"Dawidson Gomes","url":"https://ufmg.academia.edu/DawidsonGomes"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="18923908"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/18923908/Gen%C3%B3tipo_e_express%C3%A3o_da_prote%C3%ADna_fluorescente_verde_melhorada_em_ratos_da_linhagem_LEW_Tg_EGFP_F455_5_Rrrc"><img alt="Research paper thumbnail of Genótipo e expressão da proteína fluorescente verde melhorada em ratos da linhagem LEW-Tg (EGFP) F455.5/Rrrc" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/18923908/Gen%C3%B3tipo_e_express%C3%A3o_da_prote%C3%ADna_fluorescente_verde_melhorada_em_ratos_da_linhagem_LEW_Tg_EGFP_F455_5_Rrrc">Genótipo e expressão da proteína fluorescente verde melhorada em ratos da linhagem LEW-Tg (EGFP) F455.5/Rrrc</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://ufmg.academia.edu/DawidsonGomes">Dawidson Gomes</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/Nat%C3%A1liaBreyner">Natália Breyner</a></span></div><div class="wp-workCard_item"><span>Brazilian Journal of Veterinary Research and Animal Science</span><span>, 2013</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="18923908"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="18923908"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 18923908; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=18923908]").text(description); $(".js-view-count[data-work-id=18923908]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 18923908; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='18923908']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=18923908]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":18923908,"title":"Genótipo e expressão da proteína fluorescente verde melhorada em ratos da linhagem LEW-Tg (EGFP) F455.5/Rrrc","internal_url":"https://www.academia.edu/18923908/Gen%C3%B3tipo_e_express%C3%A3o_da_prote%C3%ADna_fluorescente_verde_melhorada_em_ratos_da_linhagem_LEW_Tg_EGFP_F455_5_Rrrc","owner_id":38951595,"coauthors_can_edit":true,"owner":{"id":38951595,"first_name":"Dawidson","middle_initials":null,"last_name":"Gomes","page_name":"DawidsonGomes","domain_name":"ufmg","created_at":"2015-11-23T03:22:12.839-08:00","display_name":"Dawidson Gomes","url":"https://ufmg.academia.edu/DawidsonGomes"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="18923907"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/18923907/Characterization_of_Decellularized_Heart_Matrices_as_Biomaterials_for_Regular_and_Whole_Organ_Tissue_Engineering_and_Initial_In_vitro_Recellularization_with_Ips_Cells"><img alt="Research paper thumbnail of Characterization of Decellularized Heart Matrices as Biomaterials for Regular and Whole Organ Tissue Engineering and Initial In-vitro Recellularization with Ips Cells" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/18923907/Characterization_of_Decellularized_Heart_Matrices_as_Biomaterials_for_Regular_and_Whole_Organ_Tissue_Engineering_and_Initial_In_vitro_Recellularization_with_Ips_Cells">Characterization of Decellularized Heart Matrices as Biomaterials for Regular and Whole Organ Tissue Engineering and Initial In-vitro Recellularization with Ips Cells</a></div><div class="wp-workCard_item"><span>Journal of Tissue Science & Engineering</span><span>, 2012</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Tissue engineering strategies, based on solid/porous scaffolds, suffer from several limitations, ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Tissue engineering strategies, based on solid/porous scaffolds, suffer from several limitations, such as ineffective vascularization, poor cell distribution and organization within scaffold, in addition to low final cell density, among others. Therefore, the search for other tissue engineering approaches constitutes an active area of investigation. Decellularized matrices (DM) present major advantages compared to solid scaffolds, such as ideal chemical composition, the preservation of vascularization structure and perfect three-dimensional structure. In the present study, we aimed to characterize and investigate murine heart decellularized matrices as biomaterials for regular and whole organ tissue engineering. Heart decellularized matrices were characterized according to: 1. DNA content, through DNA quantificationo and PCR of isolated genomic DNA; 2. Histological structure, assessed after Hematoxylin and Eosin, as well as Masson&amp;amp;amp;amp;amp;#39;s Trichrome stainings; 3. Surface nanostructure analysis, performed, using SEM. Those essays allowed us to conclude that DM was indeed decellularized, with preserved extracellular matrix structure. Following characterization, decellularized heart slices were seeded with induced Pluripotent Stem cells (iPS). As expected, but - to the best of our knowledge - never shown before, decellularization of murine heart matrices maintained matrix biocompatibility, as iPS cells rapidly attached to the surface of the material and proliferated. Strikingly though, heart DM presented a differentiation induction effect over those cells, which lost their pluripotency markers after 7 days of culture in the DM. Such loss of differentiation markers was observed, even though bFGF containing media mTSR was used during such period. Gene expression of iPS cells cultured on DM will be further analyzed, in order to assess the effects of culturing pluripotent stem cells in decellularized heart matrices.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="18923907"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="18923907"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 18923907; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=18923907]").text(description); $(".js-view-count[data-work-id=18923907]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 18923907; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='18923907']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=18923907]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":18923907,"title":"Characterization of Decellularized Heart Matrices as Biomaterials for Regular and Whole Organ Tissue Engineering and Initial In-vitro Recellularization with Ips Cells","internal_url":"https://www.academia.edu/18923907/Characterization_of_Decellularized_Heart_Matrices_as_Biomaterials_for_Regular_and_Whole_Organ_Tissue_Engineering_and_Initial_In_vitro_Recellularization_with_Ips_Cells","owner_id":38951595,"coauthors_can_edit":true,"owner":{"id":38951595,"first_name":"Dawidson","middle_initials":null,"last_name":"Gomes","page_name":"DawidsonGomes","domain_name":"ufmg","created_at":"2015-11-23T03:22:12.839-08:00","display_name":"Dawidson Gomes","url":"https://ufmg.academia.edu/DawidsonGomes"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="18923906"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/18923906/Stem_Cells_and_Calcium_Signaling"><img alt="Research paper thumbnail of Stem Cells and Calcium Signaling" class="work-thumbnail" src="https://attachments.academia-assets.com/40331567/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/18923906/Stem_Cells_and_Calcium_Signaling">Stem Cells and Calcium Signaling</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://ufmg.academia.edu/DawidsonGomes">Dawidson Gomes</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://usp-br.academia.edu/K%C3%A1tiaGomes">Kátia Gomes</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://helder.academia.edu/FernandaTonelli">Fernanda M P Tonelli</a></span></div><div class="wp-workCard_item"><span>Advances in Experimental Medicine and Biology</span><span>, 2012</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The increasing interest in stem cell research is linked to the promise of developing treatments f...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The increasing interest in stem cell research is linked to the promise of developing treatments for many lifethreatening, debilitating diseases, and for cell replacement therapies. However, performing these therapeutic innovations with safety will only be possible when an accurate knowledge about the molecular signals that promote the desired cell fate is reached. Among these signals are transient changes in intracellular Ca 2+ concentration [Ca 2+ ] i . Acting as an intracellular messenger, Ca 2+ has a key role in cell signaling pathways in various differentiation stages of stem cells. The aim of this chapter is to present a broad overview of various moments in which Ca 2+ -mediated signaling is essential for the maintenance of stem cells and for promoting their development and differentiation, also focusing on their therapeutic potential.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="7098012ccf958a5480c6b73023abe8fb" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":40331567,"asset_id":18923906,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/40331567/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="18923906"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="18923906"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 18923906; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=18923906]").text(description); $(".js-view-count[data-work-id=18923906]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 18923906; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='18923906']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "7098012ccf958a5480c6b73023abe8fb" } } $('.js-work-strip[data-work-id=18923906]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":18923906,"title":"Stem Cells and Calcium Signaling","internal_url":"https://www.academia.edu/18923906/Stem_Cells_and_Calcium_Signaling","owner_id":38951595,"coauthors_can_edit":true,"owner":{"id":38951595,"first_name":"Dawidson","middle_initials":null,"last_name":"Gomes","page_name":"DawidsonGomes","domain_name":"ufmg","created_at":"2015-11-23T03:22:12.839-08:00","display_name":"Dawidson Gomes","url":"https://ufmg.academia.edu/DawidsonGomes"},"attachments":[{"id":40331567,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/40331567/thumbnails/1.jpg","file_name":"Stem_Cells_and_Calcium_Signaling20151124-6941-2tvpnt.pdf","download_url":"https://www.academia.edu/attachments/40331567/download_file","bulk_download_file_name":"Stem_Cells_and_Calcium_Signaling.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/40331567/Stem_Cells_and_Calcium_Signaling20151124-6941-2tvpnt-libre.pdf?1448375341=\u0026response-content-disposition=attachment%3B+filename%3DStem_Cells_and_Calcium_Signaling.pdf\u0026Expires=1739809940\u0026Signature=aiYOpiy2qIC6bGvE96BN7fVYlBnG0D2zuLZh8k-rWmuTvb9Wt~fIEp1-Lh8n41C6pmq38l1q-ydyVpZKdiSsh6iKHbbqc3BaBSlzf-VG6BSbhJMknQroxa5C~7vUCDLFhLesWul2BvGx3ow9f5BrzU~hNsScE6uGfRTVSKRwd1CP-E0q8ywBca3Q6q5FPmMQPRvSP6SBfbj16sw~BWeWQ6NWA-6PjqAQcmEGTWN8T4s6-~hcAY9d9yiBLYRofET5ODoNDcHbkGoP7LgcluykI3-0wfD47i0zpkTmAEBFJ5oExwfmPo6W3k4vMU2CQRtseXPuV9PyRGLrhw9sdXNR4w__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="18923905"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/18923905/Immunization_with_Recombinant_Pb27_Protein_Reduces_the_Levels_of_Pulmonary_Fibrosis_Caused_by_the_Inflammatory_Response_Against_Paracoccidioides_brasiliensis"><img alt="Research paper thumbnail of Immunization with Recombinant Pb27 Protein Reduces the Levels of Pulmonary Fibrosis Caused by the Inflammatory Response Against Paracoccidioides brasiliensis" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/18923905/Immunization_with_Recombinant_Pb27_Protein_Reduces_the_Levels_of_Pulmonary_Fibrosis_Caused_by_the_Inflammatory_Response_Against_Paracoccidioides_brasiliensis">Immunization with Recombinant Pb27 Protein Reduces the Levels of Pulmonary Fibrosis Caused by the Inflammatory Response Against Paracoccidioides brasiliensis</a></div><div class="wp-workCard_item"><span>Mycopathologia</span><span>, 2014</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Paracoccidioidomycosis (PCM) is a systemic mycosis in which the host response to the infectious a...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Paracoccidioidomycosis (PCM) is a systemic mycosis in which the host response to the infectious agent typically consists of a chronic granulomatous inflammatory process. This condition causes lesions that impair lung function and lead to chronic pulmonary insufficiency resulting from fibrosis development, which is a sequel and disabling feature of the disease. The rPb27 protein has been studied for prophylactic and therapeutic treatment against PCM. Previous studies from our laboratory have shown a protective effect of rPb27 against PCM. However, these studies have not determined whether rPb27 immunization prevents lung fibrosis. We therefore conducted this study to investigate fibrosis resulting from infection by Paracoccidioides brasiliensis in the lungs of animals immunized with rPb27. Animals were immunized with rPb27 and subsequently infected with a virulent strain of P. brasiliensis. Fungal load was evaluated by counting colony-forming units, and Masson&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s trichrome staining was performed to evaluate fibrosis at 30 and 90 days post-infection. The levels of CCR7, active caspase 3, collagen and cytokines were analyzed. At the two time intervals mentioned, the rPb27 group showed lower levels of fibrosis on histology and reduced levels of collagen and the chemokine receptor CCR7 in the lungs. CCR7 was detected at higher levels in the control groups that developed very high levels of pulmonary fibrosis. Additionally, the immunized groups showed high levels of active caspase 3, IFN-γ, TGF-β and IL-10 in the early phase of P. brasiliensis infection. Immunization with Pb27, in addition to its protective effect, was shown to prevent pulmonary fibrosis.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="18923905"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="18923905"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 18923905; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=18923905]").text(description); $(".js-view-count[data-work-id=18923905]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 18923905; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='18923905']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=18923905]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":18923905,"title":"Immunization with Recombinant Pb27 Protein Reduces the Levels of Pulmonary Fibrosis Caused by the Inflammatory Response Against Paracoccidioides brasiliensis","internal_url":"https://www.academia.edu/18923905/Immunization_with_Recombinant_Pb27_Protein_Reduces_the_Levels_of_Pulmonary_Fibrosis_Caused_by_the_Inflammatory_Response_Against_Paracoccidioides_brasiliensis","owner_id":38951595,"coauthors_can_edit":true,"owner":{"id":38951595,"first_name":"Dawidson","middle_initials":null,"last_name":"Gomes","page_name":"DawidsonGomes","domain_name":"ufmg","created_at":"2015-11-23T03:22:12.839-08:00","display_name":"Dawidson Gomes","url":"https://ufmg.academia.edu/DawidsonGomes"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="18923904"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/18923904/Preserva%C3%A7%C3%A3o_da_prote%C3%ADna_verde_fluorescente_no_tecido_%C3%B3sseo_descalcificado"><img alt="Research paper thumbnail of Preservação da proteína verde fluorescente no tecido ósseo descalcificado" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/18923904/Preserva%C3%A7%C3%A3o_da_prote%C3%ADna_verde_fluorescente_no_tecido_%C3%B3sseo_descalcificado">Preservação da proteína verde fluorescente no tecido ósseo descalcificado</a></div><div class="wp-workCard_item"><span>Ciência Rural</span><span>, 2010</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">... Ciência Rural, v.40, n.10, out, 2010. Ciência Rural, Santa Maria, Online ISSN 0103-8478 Janke...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">... Ciência Rural, v.40, n.10, out, 2010. Ciência Rural, Santa Maria, Online ISSN 0103-8478 Jankerle Neves BoeloniI Natália de Melo OcarinoI Rafaela Chitarra Rodrigues HellI Guilherme Resende da SilvaI Alfredo Miranda GoesII Dawidson Assis GomesII Rogéria SerakidesI* ...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="18923904"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="18923904"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 18923904; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=18923904]").text(description); $(".js-view-count[data-work-id=18923904]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 18923904; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='18923904']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=18923904]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":18923904,"title":"Preservação da proteína verde fluorescente no tecido ósseo descalcificado","internal_url":"https://www.academia.edu/18923904/Preserva%C3%A7%C3%A3o_da_prote%C3%ADna_verde_fluorescente_no_tecido_%C3%B3sseo_descalcificado","owner_id":38951595,"coauthors_can_edit":true,"owner":{"id":38951595,"first_name":"Dawidson","middle_initials":null,"last_name":"Gomes","page_name":"DawidsonGomes","domain_name":"ufmg","created_at":"2015-11-23T03:22:12.839-08:00","display_name":"Dawidson Gomes","url":"https://ufmg.academia.edu/DawidsonGomes"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="18923903"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/18923903/Characterization_and_induction_of_cementoblast_cell_proliferation_by_bioactive_glass_nanoparticles"><img alt="Research paper thumbnail of Characterization and induction of cementoblast cell proliferation by bioactive glass nanoparticles" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/18923903/Characterization_and_induction_of_cementoblast_cell_proliferation_by_bioactive_glass_nanoparticles">Characterization and induction of cementoblast cell proliferation by bioactive glass nanoparticles</a></div><div class="wp-workCard_item"><span>Journal of Tissue Engineering and Regenerative Medicine</span><span>, 2012</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Cementum is a mineralized tissue that lines the surface of the tooth root enabling attachment of ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Cementum is a mineralized tissue that lines the surface of the tooth root enabling attachment of the periodontal ligament to the root and surrounding alveolar bone. Studies examining the mechanisms involved in the formation of root cementum have been hindered by an inability to isolate and culture the cells required for cementum production (cementoblasts). This study isolated and characterized cementoblast cells derived from rat molar periodontal ligament. It was observed that the isolated cells expressed F-Spondin, a cementoblast marker, while F-Spondin expression was not observed in the cells of other tissues such as gingival fibroblasts and osteoblasts. As expected, the isolated cementoblast cells also expressed osteocalcin (OC), bone sialoprotein (BSP), alkaline phosphatase (ALP), and type I collagen, demonstrating the presence of mineralized tissues genes in cementoblast cells. These cells showed high ALP activity and calcified nodule formation in vitro. Since cementogenesis could be a critical event for regeneration of periodontal tissues, this study investigated whether bioactive glass particles could affect the proliferation of cementoblasts since they are known to enhance osteoblast proliferation. It was found that the ionic products from bioactive glass nanoparticles increased cementoblast viability, mitochondrial activity, and induced cell proliferation. Together, these results show the characterization of cementoblast cells from rat molar periodontal ligament. Additionally, it was shown that bioactive glass nanoparticles induced cementoblast to proliferate, indicating that they could be a potential material for use in cement regeneration through tissue engineering.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="18923903"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="18923903"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 18923903; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=18923903]").text(description); $(".js-view-count[data-work-id=18923903]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 18923903; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='18923903']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); 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Treatment options abounds, although efficacy is limited. Recent studies attribute discrete and ephemeral benefits to adult stem cell therapies, indicating the urge to improve stem cell based-therapy. In this study, we show that priming mesenchymal stem cells (MSC) towards cardiomyogenic lineage enhances their beneficial effects in vivo as treatment option for acute phase myocardial infarction. MSC were primed using cardiomyogenic media for 4 days, after which peak expression of key cardiomyogenic genes are reached and protein expression of Cx-43 and sarcomeric aactinin are observed. MSC and primed MSC (pMSC) were characterized in vitro and used to treat infarcted rats immediately after left anterior descending (LAD) occlusion. Echocardiography analysis indicated that MSC-treated myocardium presented discrete improvement in function, but it also showed that pMSC treatment lead to superior beneficial results, compared with undifferentiated MSC. Seven days after cell injection, MSC and pMSC could still be detected in the myocardium. Connexin-43 expression was quantified through immunoblotting, and was superior in pMSC, indicating that this could be a possible explanation for the superior performance of pMSC therapy.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="b6d1f07b16c714385827410e3c17d6d3" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":39176918,"asset_id":16776014,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/39176918/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="16776014"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="16776014"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 16776014; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=16776014]").text(description); $(".js-view-count[data-work-id=16776014]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 16776014; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='16776014']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "b6d1f07b16c714385827410e3c17d6d3" } } $('.js-work-strip[data-work-id=16776014]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":16776014,"title":"Priming mesenchymal stem cells boosts stem cell therapy to treat myocardial infarction","internal_url":"https://www.academia.edu/16776014/Priming_mesenchymal_stem_cells_boosts_stem_cell_therapy_to_treat_myocardial_infarction","owner_id":36208103,"coauthors_can_edit":true,"owner":{"id":36208103,"first_name":"Marcos","middle_initials":null,"last_name":"Melo","page_name":"MMelo","domain_name":"ufmg","created_at":"2015-10-14T05:25:55.184-07:00","display_name":"Marcos Melo","url":"https://ufmg.academia.edu/MMelo"},"attachments":[{"id":39176918,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/39176918/thumbnails/1.jpg","file_name":"02e7e5152ea9074e4d000000.pdf","download_url":"https://www.academia.edu/attachments/39176918/download_file","bulk_download_file_name":"Priming_mesenchymal_stem_cells_boosts_st.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/39176918/02e7e5152ea9074e4d000000-libre.pdf?1444825710=\u0026response-content-disposition=attachment%3B+filename%3DPriming_mesenchymal_stem_cells_boosts_st.pdf\u0026Expires=1739827323\u0026Signature=XVeJX4bxHVCqBGwgwtLj4U8XiVMubk1Ej828jILMAJQATuXtMOWfiyHsUklgojr3bU~HZBOXa4z~L3dhUKFIaZwxe1OzZjyF9XK2DOd0a6TRA3bc8YA2yCLfIPTI4okZSijayJ39cPuoMIsQVk4HX57fhkw-oxu3QFxceg090GHU5VMXRsfUxbbgDjmZ9regMbOC-zX3NEWaQAbSTKypAf5Vj82JN80PVDg5pmyyx-oItZpEs1XGepzK2f-o2CAsiZjycEuPZnH9-ptRBprZRvTwTT5haJ33RfduC2IKH8N6H7WsIufdV-Yh~f-OT6xOP7y7EDxsHLj9NctPfyEWbQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="18923901"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/18923901/Hormonal_Regulation_of_Nuclear_Permeability"><img alt="Research paper thumbnail of Hormonal Regulation of Nuclear Permeability" class="work-thumbnail" src="https://attachments.academia-assets.com/40331574/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/18923901/Hormonal_Regulation_of_Nuclear_Permeability">Hormonal Regulation of Nuclear Permeability</a></div><div class="wp-workCard_item"><span>Journal of Biological Chemistry</span><span>, 2006</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Transport into the nucleus is critical for regulation of gene transcription and other intranuclea...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Transport into the nucleus is critical for regulation of gene transcription and other intranuclear events. Passage of molecules into the nucleus depends in part upon their size and the presence of appropriate targeting sequences. However, little is known about the effects of hormones or their second messengers on transport across the nuclear envelope. We used localized, two-photon activation of a photoactivatable green fluorescent protein to investigate whether hormones, via their second messengers, could alter nuclear permeability. Vasopressin other hormones that increase cytosolic Ca 2+ and activate protein kinase C increased permeability across the nuclear membrane of SKHep1 liver cells in a rapid unidirectional manner. An increase in cytosolic Ca 2+ was both necessary and sufficient for this process. Furthermore, localized photorelease of caged Ca 2+ near the nuclear envelope resulted in a local increase in nuclear permeability. Neither activation nor inhibition of protein kinase C affected nuclear permeability. These findings provide evidence that hormones linking to certain G protein-coupled receptors increase nuclear permeability via cytosolic Ca 2+ . Short term regulation of nuclear permeability may provide a novel mechanism by which such hormones permit transcription factors and other regulatory molecules to enter the nucleus, thereby regulating gene transcription in target cells.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="86633f740807b740f709c45766103eab" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":40331574,"asset_id":18923901,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/40331574/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="18923901"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="18923901"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 18923901; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=18923901]").text(description); $(".js-view-count[data-work-id=18923901]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 18923901; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='18923901']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "86633f740807b740f709c45766103eab" } } $('.js-work-strip[data-work-id=18923901]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":18923901,"title":"Hormonal Regulation of Nuclear Permeability","internal_url":"https://www.academia.edu/18923901/Hormonal_Regulation_of_Nuclear_Permeability","owner_id":38951595,"coauthors_can_edit":true,"owner":{"id":38951595,"first_name":"Dawidson","middle_initials":null,"last_name":"Gomes","page_name":"DawidsonGomes","domain_name":"ufmg","created_at":"2015-11-23T03:22:12.839-08:00","display_name":"Dawidson Gomes","url":"https://ufmg.academia.edu/DawidsonGomes"},"attachments":[{"id":40331574,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/40331574/thumbnails/1.jpg","file_name":"Hormonal_Regulation_of_Nuclear_Permeabil20151124-8846-4vy82u.pdf","download_url":"https://www.academia.edu/attachments/40331574/download_file","bulk_download_file_name":"Hormonal_Regulation_of_Nuclear_Permeabil.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/40331574/Hormonal_Regulation_of_Nuclear_Permeabil20151124-8846-4vy82u-libre.pdf?1448375341=\u0026response-content-disposition=attachment%3B+filename%3DHormonal_Regulation_of_Nuclear_Permeabil.pdf\u0026Expires=1739827323\u0026Signature=KkzLFIAuNQ4I-7C8UeJnnPbiSkRaYCdtdz4MB6Gx8VZxZ7SakV9qSouHxPxYkRX~0YQZAN3gKKIIL89dzjJHnYfRi4K5qwHcbeB5AEq29ZoeXqSsCt7C5Cw4E8Jj3r~tQWLCMrhtPxKzxvI10RtaWjSmj9Z5n7PKFAlBZ9MZnkxk8O4sO0xtZmtO8BRpx6jlDlZD3BOoNkFfEM1x8MwUzgGbc2xHyoF0OLS1LPDjx5EsbKSXYnW8p0Nh-AxlhpTxoK5Lo7PqP58UFyTI8AGdC7gQ6mtTl2WU0zt6vnK-20wC2Sgo1PRcKNUY8tR7HvEb5Mi-X2NXOKPnMxEwM67XWg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="18923899"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/18923899/Nucleoplasmic_Calcium_Is_Required_for_Cell_Proliferation"><img alt="Research paper thumbnail of Nucleoplasmic Calcium Is Required for Cell Proliferation" class="work-thumbnail" src="https://attachments.academia-assets.com/42151066/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/18923899/Nucleoplasmic_Calcium_Is_Required_for_Cell_Proliferation">Nucleoplasmic Calcium Is Required for Cell Proliferation</a></div><div class="wp-workCard_item"><span>Journal of Biological Chemistry</span><span>, 2007</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Ca 2+ signals regulate cell proliferation, but the spatial and temporal specificity of these sign...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Ca 2+ signals regulate cell proliferation, but the spatial and temporal specificity of these signals is unknown. Here we use selective buffers of nucleoplasmic or cytoplasmic Ca 2+ to determine that cell proliferation depends upon Ca 2+ signals within the nucleus rather than in the cytoplasm. Nuclear Ca 2+ signals stimulate cell growth rather than inhibit apoptosis and specifically permit cells to advance through early prophase. Selective buffering of nuclear but not cytoplasmic Ca 2+ signals also impairs growth of tumors in vivo. These findings reveal a major physiological and potential pathophysiological role for nucleoplasmic Ca 2+ signals and suggest that this information can be used to design novel therapeutic strategies to regulate conditions of abnormal cell growth.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="6f9c900706f538c61b2e7780b338cbf4" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":42151066,"asset_id":18923899,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/42151066/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="18923899"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="18923899"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 18923899; 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window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=18923897]").text(description); $(".js-view-count[data-work-id=18923897]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 18923897; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='18923897']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "e7229874bd18851af4554bb444810c63" } } $('.js-work-strip[data-work-id=18923897]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":18923897,"title":"The Type III Inositol 1,4,5-Trisphosphate Receptor Preferentially Transmits Apoptotic Ca2+ Signals into Mitochondria","internal_url":"https://www.academia.edu/18923897/The_Type_III_Inositol_1_4_5_Trisphosphate_Receptor_Preferentially_Transmits_Apoptotic_Ca2_Signals_into_Mitochondria","owner_id":38951595,"coauthors_can_edit":true,"owner":{"id":38951595,"first_name":"Dawidson","middle_initials":null,"last_name":"Gomes","page_name":"DawidsonGomes","domain_name":"ufmg","created_at":"2015-11-23T03:22:12.839-08:00","display_name":"Dawidson Gomes","url":"https://ufmg.academia.edu/DawidsonGomes"},"attachments":[{"id":40331648,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/40331648/thumbnails/1.jpg","file_name":"The_Type_III_Inositol_145-Trisphosphate_20151124-6941-b40mld.pdf","download_url":"https://www.academia.edu/attachments/40331648/download_file","bulk_download_file_name":"The_Type_III_Inositol_1_4_5_Trisphosphat.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/40331648/The_Type_III_Inositol_145-Trisphosphate_20151124-6941-b40mld-libre.pdf?1448375470=\u0026response-content-disposition=attachment%3B+filename%3DThe_Type_III_Inositol_1_4_5_Trisphosphat.pdf\u0026Expires=1739809940\u0026Signature=Dzyr5xkE5QSKBpXXmqdFhX9SjdHZ1GmWjskuizU0QXv1GSZg1hVUVPxBjIwWTISIIdBcY3DTvNN6iQpqmBsctIAPlE2rIzpEm41-oOuzsxFo-e4RRJNYLC3mSQmhZhVO6UeM1bA~OyGjpO5KsF819zNxTre-HoT8zlNgznQSDdXG7Q9-yNNq~5nKt9P7XVroJmTP~8AlEme4pBqtzplEuMg9d3XVGKkLGeX~WFvmLxxY3IIa0dWmUVnA70~5-PISD7INt3MJhcL4TCbsrSFLapag5wVZl5x0AhRL3a6TMb6Ti9lDiBCkHR5sF~ujULupenUdHu9~kTHXjxtwIsMA7w__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="18923895"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/18923895/Adenine_Induces_Differentiation_of_Rat_Hepatic_Stellate_Cells"><img alt="Research paper thumbnail of Adenine Induces Differentiation of Rat Hepatic Stellate Cells" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/18923895/Adenine_Induces_Differentiation_of_Rat_Hepatic_Stellate_Cells">Adenine Induces Differentiation of Rat Hepatic Stellate Cells</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/AzumaWatanabe">Azuma Watanabe</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://ufmg.academia.edu/DawidsonGomes">Dawidson Gomes</a></span></div><div class="wp-workCard_item"><span>Digestive Diseases and Sciences</span><span>, 2012</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Adenine is a uric acid pathway metabolite of no known function, and has recently been identified ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Adenine is a uric acid pathway metabolite of no known function, and has recently been identified as a ligand for a rat G protein-coupled receptor. Due to the known role of other uric acid pathway metabolites in HSC biology, we tested the ability of adenine to induce HSC differentiation. RT-PCR was performed for adenine receptor expression in T-6 and primary rat HSC. T-6 and primary rats HSC were cultured with and without adenine, and stellation examined. Next, we examined inhibition of calcium signaling using caged IP(3). To test if adenine inhibits HSC chemotaxis T-6 cells and rat HSCs were cultured with or without adenine for 24 h in a transwell assay with PDGF as the chemoattractant. cDNA was prepared from T-6 and primary HSC for quantification of collagen 1 mRNA using real-time PCR. We found that mRNA for the adenine receptor is expressed in T-6 cells and primary rat HSC. Also, adenine induces HSC stellation and adenine inhibits IP(3) mediated increase in cytosolic [Ca(2+)](i) and inhibits chemotaxis in T-6 cells and primary rat HSC. Adenine was also shown to up-regulate α-SMA and collagen 1, and this effect is lost by using specific si-RNA for the adenine receptor. Finally, adenine inhibits endothelin-1-induced gel contraction. The adenine receptor is present in T-6 cells and primary rats HSC. Adenine, via the adenine receptor, induces morphological change, and cytosolic calcium signaling, inhibits chemotaxis, and up-regulates collagen 1 mRNA in HSCs.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="18923895"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="18923895"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 18923895; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=18923895]").text(description); $(".js-view-count[data-work-id=18923895]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 18923895; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='18923895']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=18923895]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":18923895,"title":"Adenine Induces Differentiation of Rat Hepatic Stellate Cells","internal_url":"https://www.academia.edu/18923895/Adenine_Induces_Differentiation_of_Rat_Hepatic_Stellate_Cells","owner_id":38951595,"coauthors_can_edit":true,"owner":{"id":38951595,"first_name":"Dawidson","middle_initials":null,"last_name":"Gomes","page_name":"DawidsonGomes","domain_name":"ufmg","created_at":"2015-11-23T03:22:12.839-08:00","display_name":"Dawidson Gomes","url":"https://ufmg.academia.edu/DawidsonGomes"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="18852301"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/18852301/Nuclear_calcium_signaling_a_cell_within_a_cell"><img alt="Research paper thumbnail of Nuclear calcium signaling: a cell within a cell" class="work-thumbnail" src="https://attachments.academia-assets.com/40290712/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/18852301/Nuclear_calcium_signaling_a_cell_within_a_cell">Nuclear calcium signaling: a cell within a cell</a></div><div class="wp-workCard_item"><span>Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.]</span><span>, 2009</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Calcium (Ca2+) is a versatile second messenger that regulates a wide range of cellular functions....</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Calcium (Ca2+) is a versatile second messenger that regulates a wide range of cellular functions. Although it is not established how a single second messenger coordinates diverse effects within a cell, there is increasing evidence that the spatial patterns of Ca2+ signals may determine their specificity. Ca2+ signaling patterns can vary in different regions of the cell and Ca2+ signals in nuclear and cytoplasmic compartments have been reported to occur independently. No general paradigm has been established yet to explain whether, how, or when Ca2+ signals are initiated within the nucleus or their function. Here we highlight that receptor tyrosine kinases rapidly translocate to the nucleus. Ca2+ signals that are induced by growth factors result from phosphatidylinositol 4,5-bisphosphate hydrolysis and inositol 1,4,5-trisphosphate formation within the nucleus rather than within the cytoplasm. This novel signaling mechanism may be responsible for growth factor effects on cell prolifer...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="533611c8f6a84f83d27aca234b2a00e7" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":40290712,"asset_id":18852301,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/40290712/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="18852301"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="18852301"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 18852301; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=18852301]").text(description); $(".js-view-count[data-work-id=18852301]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 18852301; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='18852301']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="18852300"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/18852300/Effect_of_halothane_on_the_release_of_Ca2_i_in_dorsal_root_ganglion_neurons"><img alt="Research paper thumbnail of Effect of halothane on the release of [Ca2+]i in dorsal root ganglion neurons" class="work-thumbnail" src="https://attachments.academia-assets.com/40290702/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/18852300/Effect_of_halothane_on_the_release_of_Ca2_i_in_dorsal_root_ganglion_neurons">Effect of halothane on the release of [Ca2+]i in dorsal root ganglion neurons</a></div><div class="wp-workCard_item"><span>Neuroreport</span><span>, Jan 19, 2004</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">We investigated the effect of the volatile anaesthetic halothane on [Ca2+]i of dorsal root gangli...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">We investigated the effect of the volatile anaesthetic halothane on [Ca2+]i of dorsal root ganglion neurons. Halothane was able to increase [Ca2+]i in those neurons in a dose-dependent manner and independent of extracellular calcium. However, halothane action was inhibited by BAPTA-AM, suggesting the involvement of intracellular calcium stores. Dantrolene, an inhibitor of ryanodine-sensitive calcium stores had no effect while 2-APB, an inhibitor of IP3-sensitive calcium store reduced by 78% the halothane-evoked increase on [Ca2+]i. These data suggests that halothane increased [Ca2+]i of ganglion neurons through calcium release from IP3-sensitive calcium store. One possible consequence of the halothane action is to alter presynaptic activity and signaling pathways that influence neurotransmission.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="00927a4b7cf3e75085914766976d5c85" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":40290702,"asset_id":18852300,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/40290702/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="18852300"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="18852300"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 18852300; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> </div><div class="profile--tab_content_container js-tab-pane tab-pane" data-section-id="4115563" id="papers"><div class="js-work-strip profile--work_container" data-work-id="18923915"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/18923915/Suppression_of_Nuclear_and_Cytosolic_Calcium_Signaling_by_Targeted_Expression_of_Calretinin"><img alt="Research paper thumbnail of Suppression of Nuclear and Cytosolic Calcium Signaling by Targeted Expression of Calretinin" class="work-thumbnail" src="https://attachments.academia-assets.com/42150432/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/18923915/Suppression_of_Nuclear_and_Cytosolic_Calcium_Signaling_by_Targeted_Expression_of_Calretinin">Suppression of Nuclear and Cytosolic Calcium Signaling by Targeted Expression of Calretinin</a></div><div class="wp-workCard_item"><span>Calcium Binding Proteins</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="78ff0d6ca70e462d6935f53ad8ff9a1b" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":42150432,"asset_id":18923915,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/42150432/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="18923915"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="18923915"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 18923915; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=18923915]").text(description); $(".js-view-count[data-work-id=18923915]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 18923915; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='18923915']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="18923914"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/18923914/Characterization_of_Decellularized_Heart_Matrices_as_Biomaterials_for_Regular_and_Whole_Organ_Tissue_Engineering_and_Initial_In_vitro_Recellularization_with_Ips_Cells"><img alt="Research paper thumbnail of Characterization of Decellularized Heart Matrices as Biomaterials for Regular and Whole Organ Tissue Engineering and Initial In-vitro Recellularization with Ips Cells" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/18923914/Characterization_of_Decellularized_Heart_Matrices_as_Biomaterials_for_Regular_and_Whole_Organ_Tissue_Engineering_and_Initial_In_vitro_Recellularization_with_Ips_Cells">Characterization of Decellularized Heart Matrices as Biomaterials for Regular and Whole Organ Tissue Engineering and Initial In-vitro Recellularization with Ips Cells</a></div><div class="wp-workCard_item"><span>Journal of tissue science & engineering</span><span>, Jan 28, 2012</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Tissue engineering strategies, based on solid/porous scaffolds, suffer from several limitations, ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Tissue engineering strategies, based on solid/porous scaffolds, suffer from several limitations, such as ineffective vascularization, poor cell distribution and organization within scaffold, in addition to low final cell density, among others. Therefore, the search for other tissue engineering approaches constitutes an active area of investigation. Decellularized matrices (DM) present major advantages compared to solid scaffolds, such as ideal chemical composition, the preservation of vascularization structure and perfect three-dimensional structure. In the present study, we aimed to characterize and investigate murine heart decellularized matrices as biomaterials for regular and whole organ tissue engineering. Heart decellularized matrices were characterized according to: 1. DNA content, through DNA quantificationo and PCR of isolated genomic DNA; 2. Histological structure, assessed after Hematoxylin and Eosin, as well as Masson&#39;s Trichrome stainings; 3. Surface nanostructure a...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="18923914"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="18923914"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 18923914; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=18923914]").text(description); $(".js-view-count[data-work-id=18923914]").attr('title', description).tooltip(); 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="18923913"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/18923913/IFN_%CE%B3_Dependent_Recruitment_of_CD4_T_Cells_and_Macrophages_Contributes_to_Pathogenesis_During_Leishmania_amazonensis_Infection"><img alt="Research paper thumbnail of IFN-γ-Dependent Recruitment of CD4(+) T Cells and Macrophages Contributes to Pathogenesis During Leishmania amazonensis Infection" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/18923913/IFN_%CE%B3_Dependent_Recruitment_of_CD4_T_Cells_and_Macrophages_Contributes_to_Pathogenesis_During_Leishmania_amazonensis_Infection">IFN-γ-Dependent Recruitment of CD4(+) T Cells and Macrophages Contributes to Pathogenesis During Leishmania amazonensis Infection</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://ufmg.academia.edu/DawidsonGomes">Dawidson Gomes</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://ufmg.academia.edu/MateusEust%C3%A1quioMouraLopes">Mateus Eustáquio Moura Lopes</a></span></div><div class="wp-workCard_item"><span>Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research</span><span>, Jan 24, 2015</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Interferon gamma (IFN-γ) is a key factor in the protection of hosts against intracellular parasit...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Interferon gamma (IFN-γ) is a key factor in the protection of hosts against intracellular parasites. This cytokine induces parasite killing through nitric oxide and reactive oxygen species production by phagocytes. Surprisingly, during Leishmania amazonensis infection, IFN-γ plays controversial roles. During in vitro infections, IFN-γ induces the proliferation of the amastigote forms of L. amazonensis. However, this cytokine is not essential at the beginning of an in vivo infection. It is not clear why IFN-γ does not mediate protection during the early stages of infection. Thus, the aim of our study was to investigate the role of IFN-γ during L. amazonensis infection. We infected IFN-γ(-/-) mice in the footpad and followed the development of leishmaniasis in these mice compared with that in WT mice. CD4(+) T lymphocytes and macrophages migrated earlier to the site of infection in the WT mice, and the earlier migration of these 2 cell types was associated with lesion development and ...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="18923913"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="18923913"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 18923913; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=18923913]").text(description); $(".js-view-count[data-work-id=18923913]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 18923913; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='18923913']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); 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</script> <div class="js-work-strip profile--work_container" data-work-id="18923912"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/18923912/Verifica%C3%A7%C3%A3o_experimental_para_confirma%C3%A7%C3%A3o_de_t%C3%A9cnica_in_vitro_do_efeito_bystander_induzido_por_radia%C3%A7%C3%A3o_gama_na_linhagem_celular_CHO_K1"><img alt="Research paper thumbnail of Verificação experimental para confirmação de técnica in vitro do efeito bystander induzido por radiação gama na linhagem celular CHO-K1" class="work-thumbnail" src="https://attachments.academia-assets.com/40331578/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/18923912/Verifica%C3%A7%C3%A3o_experimental_para_confirma%C3%A7%C3%A3o_de_t%C3%A9cnica_in_vitro_do_efeito_bystander_induzido_por_radia%C3%A7%C3%A3o_gama_na_linhagem_celular_CHO_K1">Verificação experimental para confirmação de técnica in vitro do efeito bystander induzido por radiação gama na linhagem celular CHO-K1</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Verificação experimental para confirmação de técnica in vitro do efeito bystander induzido por ra...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Verificação experimental para confirmação de técnica in vitro do efeito bystander induzido por radiação gama na linhagem celular CHO-K1 O efeito bystander diz respeito a respostas biológicas detectadas em células não diretamente irradiadas, mas que são influenciadas, de alguma forma, por sinais transmitidos por células irradiadas vizinhas. Essas respostas biológicas incluem trocas entre cromátides irmãs, mutações, formação de micronúcleos, aberrações cromossômicas, carcinogêneses, além de morte por apoptose e necrose. Apesar de sua existência ser indiscutível, os mecanismos envolvidos no desencadeamento do efeito bystander ainda não foram completamente esclarecidos. Estudos anteriores comprovam que este efeito é dependente de uma grande variedade de parâmetros incluindo a dose, a taxa de dose, o tipo de radiação e tipo de célula ou tecido. O presente trabalho visa confirmar a técnica anteriormente utilizada na literatura em linhagens celulares humanas para a verificação do efeito by...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="aee2088c18554236c364ab107d59b6bc" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":40331578,"asset_id":18923912,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/40331578/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="18923912"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="18923912"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 18923912; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=18923912]").text(description); $(".js-view-count[data-work-id=18923912]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 18923912; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='18923912']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "aee2088c18554236c364ab107d59b6bc" } } $('.js-work-strip[data-work-id=18923912]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":18923912,"title":"Verificação experimental para confirmação de técnica in vitro do efeito bystander induzido por radiação gama na linhagem celular CHO-K1","internal_url":"https://www.academia.edu/18923912/Verifica%C3%A7%C3%A3o_experimental_para_confirma%C3%A7%C3%A3o_de_t%C3%A9cnica_in_vitro_do_efeito_bystander_induzido_por_radia%C3%A7%C3%A3o_gama_na_linhagem_celular_CHO_K1","owner_id":38951595,"coauthors_can_edit":true,"owner":{"id":38951595,"first_name":"Dawidson","middle_initials":null,"last_name":"Gomes","page_name":"DawidsonGomes","domain_name":"ufmg","created_at":"2015-11-23T03:22:12.839-08:00","display_name":"Dawidson Gomes","url":"https://ufmg.academia.edu/DawidsonGomes"},"attachments":[{"id":40331578,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/40331578/thumbnails/1.jpg","file_name":"Verificao_experimental_para_confirmao_de20151124-10454-fu0vx6.pdf","download_url":"https://www.academia.edu/attachments/40331578/download_file","bulk_download_file_name":"Verificacao_experimental_para_confirmaca.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/40331578/Verificao_experimental_para_confirmao_de20151124-10454-fu0vx6-libre.pdf?1448375341=\u0026response-content-disposition=attachment%3B+filename%3DVerificacao_experimental_para_confirmaca.pdf\u0026Expires=1739809940\u0026Signature=F3WyPjr3zV8WIzGdix-~-GL3GXlr7JFIviykuUeznJAAH0-mQkgoBak5pjubCRyglll6phRb57l07T4DWVnUDvtie013u5kcS-i9iy5yavxG-3p8SVk9XBmTHvrLiW7QVsBqJPj2EcBEQa8FsuE9fJfIKq4rnEW05c3NJQUCWwxyfJVLeUhKfyzsGGjmjO-tnbZIkgElH~-W8wOpvTc8mKKeECcu3~c1UJRd9oyY9~C-jVojHjxvmsTvbuuvBpgWdiUBdMHp40GE485viKY4KCw38dyNpN-gTeGTPDHSEXz~LHnjVmzI5sGoLJ5inNmqfZhM4hr9~eTAE0oPSwGzGg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="18923911"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/18923911/Protective_effect_of_green_tea_and_Arrabidea_chica_extracts_against_induced_doxorubicin_cardiotoxicity_in_vitro_without_interfering_on_antitumor_activity"><img alt="Research paper thumbnail of Protective effect of green tea and Arrabidea chica extracts against induced doxorubicin cardiotoxicity in vitro without interfering on antitumor activity" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/18923911/Protective_effect_of_green_tea_and_Arrabidea_chica_extracts_against_induced_doxorubicin_cardiotoxicity_in_vitro_without_interfering_on_antitumor_activity">Protective effect of green tea and Arrabidea chica extracts against induced doxorubicin cardiotoxicity in vitro without interfering on antitumor activity</a></div><div class="wp-workCard_item"><span>Toxicology Letters</span><span>, 2011</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="18923911"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="18923911"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 18923911; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=18923911]").text(description); $(".js-view-count[data-work-id=18923911]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 18923911; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='18923911']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=18923911]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":18923911,"title":"Protective effect of green tea and Arrabidea chica extracts against induced doxorubicin cardiotoxicity in vitro without interfering on antitumor activity","internal_url":"https://www.academia.edu/18923911/Protective_effect_of_green_tea_and_Arrabidea_chica_extracts_against_induced_doxorubicin_cardiotoxicity_in_vitro_without_interfering_on_antitumor_activity","owner_id":38951595,"coauthors_can_edit":true,"owner":{"id":38951595,"first_name":"Dawidson","middle_initials":null,"last_name":"Gomes","page_name":"DawidsonGomes","domain_name":"ufmg","created_at":"2015-11-23T03:22:12.839-08:00","display_name":"Dawidson Gomes","url":"https://ufmg.academia.edu/DawidsonGomes"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="18923910"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/18923910/Production_of_Human_Endothelial_Cells_Free_from_Soluble_Xenogeneic_Antigens_for_Bioartificial_Small_Diameter_Vascular_Graft_Endothelization"><img alt="Research paper thumbnail of Production of Human Endothelial Cells Free from Soluble Xenogeneic Antigens for Bioartificial Small Diameter Vascular Graft Endothelization" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/18923910/Production_of_Human_Endothelial_Cells_Free_from_Soluble_Xenogeneic_Antigens_for_Bioartificial_Small_Diameter_Vascular_Graft_Endothelization">Production of Human Endothelial Cells Free from Soluble Xenogeneic Antigens for Bioartificial Small Diameter Vascular Graft Endothelization</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://ufmg.academia.edu/DawidsonGomes">Dawidson Gomes</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/Tha%C3%ADsMariaMataMartins">Thaís Maria Mata Martins</a></span></div><div class="wp-workCard_item"><span>BioMed Research International</span><span>, 2015</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Arterial bypass graft implantation remains the primary therapy for patients with advanced cardiov...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Arterial bypass graft implantation remains the primary therapy for patients with advanced cardiovascular disease, but most lack adequate saphenous vein or other conduits for bypass procedures and would benefit from a bioartificial conduit. This study aimed to produce human endothelial cells (hECs) in large scale, free from xenogeneic antigens, to develop a small diameter, compatible vessel for potential use as a vascular graft. Human adipose-derived stromal cells (hASCs) were isolated, cultured, and differentiated in the presence of human serum and used for the reendothelization of a decellularized rat aorta. hASC derived ECs (hASC-ECs) expressed VEGFR2, vWf and CD31 endothelial cell markers, the latter in higher levels than hASCs and HUVECs, and were shown to be functional. Decellularization protocol yielded aortas devoid of cell nuclei, with preserved structure, including a preserved basement membrane. When seeded with hASC-ECs, the decellularized aorta was completely reendothelized, and the hASC-ECs maintained their phenotype in this new condition. hASCs can be differentiated into functional hECs without the use of animal supplements and are capable of reendothelizing a decellularized rat aorta while maintaining their phenotype. The preservation of the basement membrane following decellularization supported the complete reendothelization of the scaffold with no cell migration towards other layers. This approach is potentially useful for rapid obtention of compatible, xenogeneic-free conduit.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="18923910"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="18923910"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 18923910; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=18923910]").text(description); $(".js-view-count[data-work-id=18923910]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 18923910; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='18923910']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=18923910]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":18923910,"title":"Production of Human Endothelial Cells Free from Soluble Xenogeneic Antigens for Bioartificial Small Diameter Vascular Graft Endothelization","internal_url":"https://www.academia.edu/18923910/Production_of_Human_Endothelial_Cells_Free_from_Soluble_Xenogeneic_Antigens_for_Bioartificial_Small_Diameter_Vascular_Graft_Endothelization","owner_id":38951595,"coauthors_can_edit":true,"owner":{"id":38951595,"first_name":"Dawidson","middle_initials":null,"last_name":"Gomes","page_name":"DawidsonGomes","domain_name":"ufmg","created_at":"2015-11-23T03:22:12.839-08:00","display_name":"Dawidson Gomes","url":"https://ufmg.academia.edu/DawidsonGomes"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="18923909"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/18923909/Nuclear_localization_of_epidermal_growth_factor_receptor_EGFR_in_ameloblastomas"><img alt="Research paper thumbnail of Nuclear localization of epidermal growth factor receptor (EGFR) in ameloblastomas" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/18923909/Nuclear_localization_of_epidermal_growth_factor_receptor_EGFR_in_ameloblastomas">Nuclear localization of epidermal growth factor receptor (EGFR) in ameloblastomas</a></div><div class="wp-workCard_item"><span>Oncotarget</span><span>, Jan 30, 2015</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Ameloblastoma is a locally invasive neoplasm often associated with morbidity and facial deformiti...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Ameloblastoma is a locally invasive neoplasm often associated with morbidity and facial deformities, showing increased Epidermal Growth Factor Receptor (EGFR) expression. Inhibition of EGFR was suggested as a treatment option for a subset of ameloblastomas. However, there are resistance mechanisms that impair anti-EGFR therapies. One important resistance mechanism for EGFR-inhibition is the EGFR nuclear localization, which activates genes responsible for its mitogenic effects, such as Cyclin D1. We assessed EGFR nuclear localization in encapsulated (unicystic, n = 3) and infiltrative (multicystic, n = 11) ameloblastomas and its colocalization with Cyclin D1 by using anti-EGFR and anti-lamin B1 double labeling immunofluorescence analyzed by confocal microscopy. Oral inflammatory fibrous hyperplasia and oral squamous cell carcinoma samples were used for comparison. Twelve cases of ameloblastoma exhibited nuclear EGFR colocalization with lamin B1. This positive staining was mainly obse...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="18923909"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="18923909"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 18923909; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=18923909]").text(description); $(".js-view-count[data-work-id=18923909]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 18923909; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='18923909']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=18923909]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":18923909,"title":"Nuclear localization of epidermal growth factor receptor (EGFR) in ameloblastomas","internal_url":"https://www.academia.edu/18923909/Nuclear_localization_of_epidermal_growth_factor_receptor_EGFR_in_ameloblastomas","owner_id":38951595,"coauthors_can_edit":true,"owner":{"id":38951595,"first_name":"Dawidson","middle_initials":null,"last_name":"Gomes","page_name":"DawidsonGomes","domain_name":"ufmg","created_at":"2015-11-23T03:22:12.839-08:00","display_name":"Dawidson Gomes","url":"https://ufmg.academia.edu/DawidsonGomes"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="18923908"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/18923908/Gen%C3%B3tipo_e_express%C3%A3o_da_prote%C3%ADna_fluorescente_verde_melhorada_em_ratos_da_linhagem_LEW_Tg_EGFP_F455_5_Rrrc"><img alt="Research paper thumbnail of Genótipo e expressão da proteína fluorescente verde melhorada em ratos da linhagem LEW-Tg (EGFP) F455.5/Rrrc" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/18923908/Gen%C3%B3tipo_e_express%C3%A3o_da_prote%C3%ADna_fluorescente_verde_melhorada_em_ratos_da_linhagem_LEW_Tg_EGFP_F455_5_Rrrc">Genótipo e expressão da proteína fluorescente verde melhorada em ratos da linhagem LEW-Tg (EGFP) F455.5/Rrrc</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://ufmg.academia.edu/DawidsonGomes">Dawidson Gomes</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/Nat%C3%A1liaBreyner">Natália Breyner</a></span></div><div class="wp-workCard_item"><span>Brazilian Journal of Veterinary Research and Animal Science</span><span>, 2013</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="18923908"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="18923908"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 18923908; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=18923908]").text(description); $(".js-view-count[data-work-id=18923908]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 18923908; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='18923908']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); 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</script> <div class="js-work-strip profile--work_container" data-work-id="18923907"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/18923907/Characterization_of_Decellularized_Heart_Matrices_as_Biomaterials_for_Regular_and_Whole_Organ_Tissue_Engineering_and_Initial_In_vitro_Recellularization_with_Ips_Cells"><img alt="Research paper thumbnail of Characterization of Decellularized Heart Matrices as Biomaterials for Regular and Whole Organ Tissue Engineering and Initial In-vitro Recellularization with Ips Cells" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/18923907/Characterization_of_Decellularized_Heart_Matrices_as_Biomaterials_for_Regular_and_Whole_Organ_Tissue_Engineering_and_Initial_In_vitro_Recellularization_with_Ips_Cells">Characterization of Decellularized Heart Matrices as Biomaterials for Regular and Whole Organ Tissue Engineering and Initial In-vitro Recellularization with Ips Cells</a></div><div class="wp-workCard_item"><span>Journal of Tissue Science & Engineering</span><span>, 2012</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Tissue engineering strategies, based on solid/porous scaffolds, suffer from several limitations, ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Tissue engineering strategies, based on solid/porous scaffolds, suffer from several limitations, such as ineffective vascularization, poor cell distribution and organization within scaffold, in addition to low final cell density, among others. Therefore, the search for other tissue engineering approaches constitutes an active area of investigation. Decellularized matrices (DM) present major advantages compared to solid scaffolds, such as ideal chemical composition, the preservation of vascularization structure and perfect three-dimensional structure. In the present study, we aimed to characterize and investigate murine heart decellularized matrices as biomaterials for regular and whole organ tissue engineering. Heart decellularized matrices were characterized according to: 1. DNA content, through DNA quantificationo and PCR of isolated genomic DNA; 2. Histological structure, assessed after Hematoxylin and Eosin, as well as Masson&amp;amp;amp;amp;amp;#39;s Trichrome stainings; 3. Surface nanostructure analysis, performed, using SEM. Those essays allowed us to conclude that DM was indeed decellularized, with preserved extracellular matrix structure. Following characterization, decellularized heart slices were seeded with induced Pluripotent Stem cells (iPS). As expected, but - to the best of our knowledge - never shown before, decellularization of murine heart matrices maintained matrix biocompatibility, as iPS cells rapidly attached to the surface of the material and proliferated. Strikingly though, heart DM presented a differentiation induction effect over those cells, which lost their pluripotency markers after 7 days of culture in the DM. Such loss of differentiation markers was observed, even though bFGF containing media mTSR was used during such period. Gene expression of iPS cells cultured on DM will be further analyzed, in order to assess the effects of culturing pluripotent stem cells in decellularized heart matrices.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="18923907"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="18923907"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 18923907; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=18923907]").text(description); $(".js-view-count[data-work-id=18923907]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 18923907; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='18923907']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="18923906"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/18923906/Stem_Cells_and_Calcium_Signaling"><img alt="Research paper thumbnail of Stem Cells and Calcium Signaling" class="work-thumbnail" src="https://attachments.academia-assets.com/40331567/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/18923906/Stem_Cells_and_Calcium_Signaling">Stem Cells and Calcium Signaling</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://ufmg.academia.edu/DawidsonGomes">Dawidson Gomes</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://usp-br.academia.edu/K%C3%A1tiaGomes">Kátia Gomes</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://helder.academia.edu/FernandaTonelli">Fernanda M P Tonelli</a></span></div><div class="wp-workCard_item"><span>Advances in Experimental Medicine and Biology</span><span>, 2012</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The increasing interest in stem cell research is linked to the promise of developing treatments f...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The increasing interest in stem cell research is linked to the promise of developing treatments for many lifethreatening, debilitating diseases, and for cell replacement therapies. However, performing these therapeutic innovations with safety will only be possible when an accurate knowledge about the molecular signals that promote the desired cell fate is reached. Among these signals are transient changes in intracellular Ca 2+ concentration [Ca 2+ ] i . Acting as an intracellular messenger, Ca 2+ has a key role in cell signaling pathways in various differentiation stages of stem cells. The aim of this chapter is to present a broad overview of various moments in which Ca 2+ -mediated signaling is essential for the maintenance of stem cells and for promoting their development and differentiation, also focusing on their therapeutic potential.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="7098012ccf958a5480c6b73023abe8fb" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":40331567,"asset_id":18923906,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/40331567/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="18923906"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="18923906"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 18923906; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=18923906]").text(description); $(".js-view-count[data-work-id=18923906]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 18923906; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='18923906']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="18923905"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/18923905/Immunization_with_Recombinant_Pb27_Protein_Reduces_the_Levels_of_Pulmonary_Fibrosis_Caused_by_the_Inflammatory_Response_Against_Paracoccidioides_brasiliensis"><img alt="Research paper thumbnail of Immunization with Recombinant Pb27 Protein Reduces the Levels of Pulmonary Fibrosis Caused by the Inflammatory Response Against Paracoccidioides brasiliensis" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/18923905/Immunization_with_Recombinant_Pb27_Protein_Reduces_the_Levels_of_Pulmonary_Fibrosis_Caused_by_the_Inflammatory_Response_Against_Paracoccidioides_brasiliensis">Immunization with Recombinant Pb27 Protein Reduces the Levels of Pulmonary Fibrosis Caused by the Inflammatory Response Against Paracoccidioides brasiliensis</a></div><div class="wp-workCard_item"><span>Mycopathologia</span><span>, 2014</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Paracoccidioidomycosis (PCM) is a systemic mycosis in which the host response to the infectious a...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Paracoccidioidomycosis (PCM) is a systemic mycosis in which the host response to the infectious agent typically consists of a chronic granulomatous inflammatory process. This condition causes lesions that impair lung function and lead to chronic pulmonary insufficiency resulting from fibrosis development, which is a sequel and disabling feature of the disease. The rPb27 protein has been studied for prophylactic and therapeutic treatment against PCM. Previous studies from our laboratory have shown a protective effect of rPb27 against PCM. However, these studies have not determined whether rPb27 immunization prevents lung fibrosis. We therefore conducted this study to investigate fibrosis resulting from infection by Paracoccidioides brasiliensis in the lungs of animals immunized with rPb27. Animals were immunized with rPb27 and subsequently infected with a virulent strain of P. brasiliensis. Fungal load was evaluated by counting colony-forming units, and Masson&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s trichrome staining was performed to evaluate fibrosis at 30 and 90 days post-infection. The levels of CCR7, active caspase 3, collagen and cytokines were analyzed. At the two time intervals mentioned, the rPb27 group showed lower levels of fibrosis on histology and reduced levels of collagen and the chemokine receptor CCR7 in the lungs. CCR7 was detected at higher levels in the control groups that developed very high levels of pulmonary fibrosis. Additionally, the immunized groups showed high levels of active caspase 3, IFN-γ, TGF-β and IL-10 in the early phase of P. brasiliensis infection. Immunization with Pb27, in addition to its protective effect, was shown to prevent pulmonary fibrosis.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="18923905"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="18923905"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 18923905; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=18923905]").text(description); $(".js-view-count[data-work-id=18923905]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 18923905; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='18923905']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=18923905]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":18923905,"title":"Immunization with Recombinant Pb27 Protein Reduces the Levels of Pulmonary Fibrosis Caused by the Inflammatory Response Against Paracoccidioides brasiliensis","internal_url":"https://www.academia.edu/18923905/Immunization_with_Recombinant_Pb27_Protein_Reduces_the_Levels_of_Pulmonary_Fibrosis_Caused_by_the_Inflammatory_Response_Against_Paracoccidioides_brasiliensis","owner_id":38951595,"coauthors_can_edit":true,"owner":{"id":38951595,"first_name":"Dawidson","middle_initials":null,"last_name":"Gomes","page_name":"DawidsonGomes","domain_name":"ufmg","created_at":"2015-11-23T03:22:12.839-08:00","display_name":"Dawidson Gomes","url":"https://ufmg.academia.edu/DawidsonGomes"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="18923904"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/18923904/Preserva%C3%A7%C3%A3o_da_prote%C3%ADna_verde_fluorescente_no_tecido_%C3%B3sseo_descalcificado"><img alt="Research paper thumbnail of Preservação da proteína verde fluorescente no tecido ósseo descalcificado" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/18923904/Preserva%C3%A7%C3%A3o_da_prote%C3%ADna_verde_fluorescente_no_tecido_%C3%B3sseo_descalcificado">Preservação da proteína verde fluorescente no tecido ósseo descalcificado</a></div><div class="wp-workCard_item"><span>Ciência Rural</span><span>, 2010</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">... Ciência Rural, v.40, n.10, out, 2010. Ciência Rural, Santa Maria, Online ISSN 0103-8478 Janke...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">... Ciência Rural, v.40, n.10, out, 2010. Ciência Rural, Santa Maria, Online ISSN 0103-8478 Jankerle Neves BoeloniI Natália de Melo OcarinoI Rafaela Chitarra Rodrigues HellI Guilherme Resende da SilvaI Alfredo Miranda GoesII Dawidson Assis GomesII Rogéria SerakidesI* ...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="18923904"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="18923904"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 18923904; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=18923904]").text(description); $(".js-view-count[data-work-id=18923904]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 18923904; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='18923904']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=18923904]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":18923904,"title":"Preservação da proteína verde fluorescente no tecido ósseo descalcificado","internal_url":"https://www.academia.edu/18923904/Preserva%C3%A7%C3%A3o_da_prote%C3%ADna_verde_fluorescente_no_tecido_%C3%B3sseo_descalcificado","owner_id":38951595,"coauthors_can_edit":true,"owner":{"id":38951595,"first_name":"Dawidson","middle_initials":null,"last_name":"Gomes","page_name":"DawidsonGomes","domain_name":"ufmg","created_at":"2015-11-23T03:22:12.839-08:00","display_name":"Dawidson Gomes","url":"https://ufmg.academia.edu/DawidsonGomes"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="18923903"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/18923903/Characterization_and_induction_of_cementoblast_cell_proliferation_by_bioactive_glass_nanoparticles"><img alt="Research paper thumbnail of Characterization and induction of cementoblast cell proliferation by bioactive glass nanoparticles" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/18923903/Characterization_and_induction_of_cementoblast_cell_proliferation_by_bioactive_glass_nanoparticles">Characterization and induction of cementoblast cell proliferation by bioactive glass nanoparticles</a></div><div class="wp-workCard_item"><span>Journal of Tissue Engineering and Regenerative Medicine</span><span>, 2012</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Cementum is a mineralized tissue that lines the surface of the tooth root enabling attachment of ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Cementum is a mineralized tissue that lines the surface of the tooth root enabling attachment of the periodontal ligament to the root and surrounding alveolar bone. Studies examining the mechanisms involved in the formation of root cementum have been hindered by an inability to isolate and culture the cells required for cementum production (cementoblasts). This study isolated and characterized cementoblast cells derived from rat molar periodontal ligament. It was observed that the isolated cells expressed F-Spondin, a cementoblast marker, while F-Spondin expression was not observed in the cells of other tissues such as gingival fibroblasts and osteoblasts. As expected, the isolated cementoblast cells also expressed osteocalcin (OC), bone sialoprotein (BSP), alkaline phosphatase (ALP), and type I collagen, demonstrating the presence of mineralized tissues genes in cementoblast cells. These cells showed high ALP activity and calcified nodule formation in vitro. Since cementogenesis could be a critical event for regeneration of periodontal tissues, this study investigated whether bioactive glass particles could affect the proliferation of cementoblasts since they are known to enhance osteoblast proliferation. It was found that the ionic products from bioactive glass nanoparticles increased cementoblast viability, mitochondrial activity, and induced cell proliferation. Together, these results show the characterization of cementoblast cells from rat molar periodontal ligament. Additionally, it was shown that bioactive glass nanoparticles induced cementoblast to proliferate, indicating that they could be a potential material for use in cement regeneration through tissue engineering.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="18923903"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="18923903"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 18923903; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=18923903]").text(description); $(".js-view-count[data-work-id=18923903]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 18923903; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='18923903']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=18923903]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":18923903,"title":"Characterization and induction of cementoblast cell proliferation by bioactive glass nanoparticles","internal_url":"https://www.academia.edu/18923903/Characterization_and_induction_of_cementoblast_cell_proliferation_by_bioactive_glass_nanoparticles","owner_id":38951595,"coauthors_can_edit":true,"owner":{"id":38951595,"first_name":"Dawidson","middle_initials":null,"last_name":"Gomes","page_name":"DawidsonGomes","domain_name":"ufmg","created_at":"2015-11-23T03:22:12.839-08:00","display_name":"Dawidson Gomes","url":"https://ufmg.academia.edu/DawidsonGomes"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="16776014"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/16776014/Priming_mesenchymal_stem_cells_boosts_stem_cell_therapy_to_treat_myocardial_infarction"><img alt="Research paper thumbnail of Priming mesenchymal stem cells boosts stem cell therapy to treat myocardial infarction" class="work-thumbnail" src="https://attachments.academia-assets.com/39176918/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/16776014/Priming_mesenchymal_stem_cells_boosts_stem_cell_therapy_to_treat_myocardial_infarction">Priming mesenchymal stem cells boosts stem cell therapy to treat myocardial infarction</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://ufmg.academia.edu/MMelo">Marcos Melo</a>, <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/MairaOliveira9">Maira Oliveira</a>, and <a class="" data-click-track="profile-work-strip-authors" href="https://ufmg.academia.edu/DawidsonGomes">Dawidson Gomes</a></span></div><div class="wp-workCard_item"><span>Journal of Cellular and Molecular Medicine</span><span>, 2013</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Cardiovascular diseases are the number one cause of death globally and are projected to remain th...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Cardiovascular diseases are the number one cause of death globally and are projected to remain the single leading cause of death. Treatment options abounds, although efficacy is limited. Recent studies attribute discrete and ephemeral benefits to adult stem cell therapies, indicating the urge to improve stem cell based-therapy. In this study, we show that priming mesenchymal stem cells (MSC) towards cardiomyogenic lineage enhances their beneficial effects in vivo as treatment option for acute phase myocardial infarction. MSC were primed using cardiomyogenic media for 4 days, after which peak expression of key cardiomyogenic genes are reached and protein expression of Cx-43 and sarcomeric aactinin are observed. MSC and primed MSC (pMSC) were characterized in vitro and used to treat infarcted rats immediately after left anterior descending (LAD) occlusion. Echocardiography analysis indicated that MSC-treated myocardium presented discrete improvement in function, but it also showed that pMSC treatment lead to superior beneficial results, compared with undifferentiated MSC. Seven days after cell injection, MSC and pMSC could still be detected in the myocardium. Connexin-43 expression was quantified through immunoblotting, and was superior in pMSC, indicating that this could be a possible explanation for the superior performance of pMSC therapy.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="b6d1f07b16c714385827410e3c17d6d3" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":39176918,"asset_id":16776014,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/39176918/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="16776014"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="16776014"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 16776014; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="18923901"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/18923901/Hormonal_Regulation_of_Nuclear_Permeability"><img alt="Research paper thumbnail of Hormonal Regulation of Nuclear Permeability" class="work-thumbnail" src="https://attachments.academia-assets.com/40331574/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/18923901/Hormonal_Regulation_of_Nuclear_Permeability">Hormonal Regulation of Nuclear Permeability</a></div><div class="wp-workCard_item"><span>Journal of Biological Chemistry</span><span>, 2006</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Transport into the nucleus is critical for regulation of gene transcription and other intranuclea...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Transport into the nucleus is critical for regulation of gene transcription and other intranuclear events. Passage of molecules into the nucleus depends in part upon their size and the presence of appropriate targeting sequences. However, little is known about the effects of hormones or their second messengers on transport across the nuclear envelope. We used localized, two-photon activation of a photoactivatable green fluorescent protein to investigate whether hormones, via their second messengers, could alter nuclear permeability. Vasopressin other hormones that increase cytosolic Ca 2+ and activate protein kinase C increased permeability across the nuclear membrane of SKHep1 liver cells in a rapid unidirectional manner. An increase in cytosolic Ca 2+ was both necessary and sufficient for this process. Furthermore, localized photorelease of caged Ca 2+ near the nuclear envelope resulted in a local increase in nuclear permeability. Neither activation nor inhibition of protein kinase C affected nuclear permeability. These findings provide evidence that hormones linking to certain G protein-coupled receptors increase nuclear permeability via cytosolic Ca 2+ . Short term regulation of nuclear permeability may provide a novel mechanism by which such hormones permit transcription factors and other regulatory molecules to enter the nucleus, thereby regulating gene transcription in target cells.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="86633f740807b740f709c45766103eab" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":40331574,"asset_id":18923901,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/40331574/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="18923901"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="18923901"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 18923901; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="18923899"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/18923899/Nucleoplasmic_Calcium_Is_Required_for_Cell_Proliferation"><img alt="Research paper thumbnail of Nucleoplasmic Calcium Is Required for Cell Proliferation" class="work-thumbnail" src="https://attachments.academia-assets.com/42151066/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/18923899/Nucleoplasmic_Calcium_Is_Required_for_Cell_Proliferation">Nucleoplasmic Calcium Is Required for Cell Proliferation</a></div><div class="wp-workCard_item"><span>Journal of Biological Chemistry</span><span>, 2007</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Ca 2+ signals regulate cell proliferation, but the spatial and temporal specificity of these sign...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Ca 2+ signals regulate cell proliferation, but the spatial and temporal specificity of these signals is unknown. Here we use selective buffers of nucleoplasmic or cytoplasmic Ca 2+ to determine that cell proliferation depends upon Ca 2+ signals within the nucleus rather than in the cytoplasm. Nuclear Ca 2+ signals stimulate cell growth rather than inhibit apoptosis and specifically permit cells to advance through early prophase. Selective buffering of nuclear but not cytoplasmic Ca 2+ signals also impairs growth of tumors in vivo. These findings reveal a major physiological and potential pathophysiological role for nucleoplasmic Ca 2+ signals and suggest that this information can be used to design novel therapeutic strategies to regulate conditions of abnormal cell growth.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="6f9c900706f538c61b2e7780b338cbf4" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":42151066,"asset_id":18923899,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/42151066/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="18923899"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="18923899"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 18923899; 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dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "6f9c900706f538c61b2e7780b338cbf4" } } $('.js-work-strip[data-work-id=18923899]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":18923899,"title":"Nucleoplasmic Calcium Is Required for Cell Proliferation","internal_url":"https://www.academia.edu/18923899/Nucleoplasmic_Calcium_Is_Required_for_Cell_Proliferation","owner_id":38951595,"coauthors_can_edit":true,"owner":{"id":38951595,"first_name":"Dawidson","middle_initials":null,"last_name":"Gomes","page_name":"DawidsonGomes","domain_name":"ufmg","created_at":"2015-11-23T03:22:12.839-08:00","display_name":"Dawidson Gomes","url":"https://ufmg.academia.edu/DawidsonGomes"},"attachments":[{"id":42151066,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/42151066/thumbnails/1.jpg","file_name":"Nucleoplasmic_Calcium_Is_Required_for_Ce20160205-30232-1sr9979.pdf","download_url":"https://www.academia.edu/attachments/42151066/download_file","bulk_download_file_name":"Nucleoplasmic_Calcium_Is_Required_for_Ce.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/42151066/Nucleoplasmic_Calcium_Is_Required_for_Ce20160205-30232-1sr9979-libre.pdf?1454684467=\u0026response-content-disposition=attachment%3B+filename%3DNucleoplasmic_Calcium_Is_Required_for_Ce.pdf\u0026Expires=1739809940\u0026Signature=DVAsk0QOG-gm1WU1eaSKUnlyuqFx8S3sGlCjvBCvBluFSvs~Fz0FBsmEEwDXf-KfKj31SwY8HxMiliEyp2SAuAkZ3Ce8aR2mVxQCgUJz9cZDQj1bqOH05R8~yF7Hs4u1a9jJE-ExBWdnxCmy50VbYeyuU41Kc37Aaah0pQ~y-pANrwZTzTWMEZN96n1gskBur1HPrH7Oua6QNShrfOgOp~UuRNH1twfiRFDyb-5A1O8zLLyvgiwPjJ2iWhCEu7Iy33w5PGyDbY1xVF8EuJAVekAXASqMYszTLEYUoHhImhyt3DUEnvqXrRua-dkPl-pVdZ6x-jtiuNRkw8DAKz~ClA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="18923897"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/18923897/The_Type_III_Inositol_1_4_5_Trisphosphate_Receptor_Preferentially_Transmits_Apoptotic_Ca2_Signals_into_Mitochondria"><img alt="Research paper thumbnail of The Type III Inositol 1,4,5-Trisphosphate Receptor Preferentially Transmits Apoptotic Ca2+ Signals into Mitochondria" class="work-thumbnail" src="https://attachments.academia-assets.com/40331648/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/18923897/The_Type_III_Inositol_1_4_5_Trisphosphate_Receptor_Preferentially_Transmits_Apoptotic_Ca2_Signals_into_Mitochondria">The Type III Inositol 1,4,5-Trisphosphate Receptor Preferentially Transmits Apoptotic Ca2+ Signals into Mitochondria</a></div><div class="wp-workCard_item"><span>Journal of Biological Chemistry</span><span>, 2005</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="e7229874bd18851af4554bb444810c63" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":40331648,"asset_id":18923897,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/40331648/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="18923897"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="18923897"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 18923897; 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dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "e7229874bd18851af4554bb444810c63" } } $('.js-work-strip[data-work-id=18923897]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":18923897,"title":"The Type III Inositol 1,4,5-Trisphosphate Receptor Preferentially Transmits Apoptotic Ca2+ Signals into Mitochondria","internal_url":"https://www.academia.edu/18923897/The_Type_III_Inositol_1_4_5_Trisphosphate_Receptor_Preferentially_Transmits_Apoptotic_Ca2_Signals_into_Mitochondria","owner_id":38951595,"coauthors_can_edit":true,"owner":{"id":38951595,"first_name":"Dawidson","middle_initials":null,"last_name":"Gomes","page_name":"DawidsonGomes","domain_name":"ufmg","created_at":"2015-11-23T03:22:12.839-08:00","display_name":"Dawidson Gomes","url":"https://ufmg.academia.edu/DawidsonGomes"},"attachments":[{"id":40331648,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/40331648/thumbnails/1.jpg","file_name":"The_Type_III_Inositol_145-Trisphosphate_20151124-6941-b40mld.pdf","download_url":"https://www.academia.edu/attachments/40331648/download_file","bulk_download_file_name":"The_Type_III_Inositol_1_4_5_Trisphosphat.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/40331648/The_Type_III_Inositol_145-Trisphosphate_20151124-6941-b40mld-libre.pdf?1448375470=\u0026response-content-disposition=attachment%3B+filename%3DThe_Type_III_Inositol_1_4_5_Trisphosphat.pdf\u0026Expires=1739809940\u0026Signature=Dzyr5xkE5QSKBpXXmqdFhX9SjdHZ1GmWjskuizU0QXv1GSZg1hVUVPxBjIwWTISIIdBcY3DTvNN6iQpqmBsctIAPlE2rIzpEm41-oOuzsxFo-e4RRJNYLC3mSQmhZhVO6UeM1bA~OyGjpO5KsF819zNxTre-HoT8zlNgznQSDdXG7Q9-yNNq~5nKt9P7XVroJmTP~8AlEme4pBqtzplEuMg9d3XVGKkLGeX~WFvmLxxY3IIa0dWmUVnA70~5-PISD7INt3MJhcL4TCbsrSFLapag5wVZl5x0AhRL3a6TMb6Ti9lDiBCkHR5sF~ujULupenUdHu9~kTHXjxtwIsMA7w__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="18923895"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/18923895/Adenine_Induces_Differentiation_of_Rat_Hepatic_Stellate_Cells"><img alt="Research paper thumbnail of Adenine Induces Differentiation of Rat Hepatic Stellate Cells" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/18923895/Adenine_Induces_Differentiation_of_Rat_Hepatic_Stellate_Cells">Adenine Induces Differentiation of Rat Hepatic Stellate Cells</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/AzumaWatanabe">Azuma Watanabe</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://ufmg.academia.edu/DawidsonGomes">Dawidson Gomes</a></span></div><div class="wp-workCard_item"><span>Digestive Diseases and Sciences</span><span>, 2012</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Adenine is a uric acid pathway metabolite of no known function, and has recently been identified ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Adenine is a uric acid pathway metabolite of no known function, and has recently been identified as a ligand for a rat G protein-coupled receptor. Due to the known role of other uric acid pathway metabolites in HSC biology, we tested the ability of adenine to induce HSC differentiation. RT-PCR was performed for adenine receptor expression in T-6 and primary rat HSC. T-6 and primary rats HSC were cultured with and without adenine, and stellation examined. Next, we examined inhibition of calcium signaling using caged IP(3). To test if adenine inhibits HSC chemotaxis T-6 cells and rat HSCs were cultured with or without adenine for 24 h in a transwell assay with PDGF as the chemoattractant. cDNA was prepared from T-6 and primary HSC for quantification of collagen 1 mRNA using real-time PCR. We found that mRNA for the adenine receptor is expressed in T-6 cells and primary rat HSC. Also, adenine induces HSC stellation and adenine inhibits IP(3) mediated increase in cytosolic [Ca(2+)](i) and inhibits chemotaxis in T-6 cells and primary rat HSC. Adenine was also shown to up-regulate α-SMA and collagen 1, and this effect is lost by using specific si-RNA for the adenine receptor. Finally, adenine inhibits endothelin-1-induced gel contraction. The adenine receptor is present in T-6 cells and primary rats HSC. Adenine, via the adenine receptor, induces morphological change, and cytosolic calcium signaling, inhibits chemotaxis, and up-regulates collagen 1 mRNA in HSCs.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="18923895"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="18923895"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 18923895; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=18923895]").text(description); $(".js-view-count[data-work-id=18923895]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 18923895; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='18923895']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=18923895]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":18923895,"title":"Adenine Induces Differentiation of Rat Hepatic Stellate Cells","internal_url":"https://www.academia.edu/18923895/Adenine_Induces_Differentiation_of_Rat_Hepatic_Stellate_Cells","owner_id":38951595,"coauthors_can_edit":true,"owner":{"id":38951595,"first_name":"Dawidson","middle_initials":null,"last_name":"Gomes","page_name":"DawidsonGomes","domain_name":"ufmg","created_at":"2015-11-23T03:22:12.839-08:00","display_name":"Dawidson Gomes","url":"https://ufmg.academia.edu/DawidsonGomes"},"attachments":[]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="18852301"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/18852301/Nuclear_calcium_signaling_a_cell_within_a_cell"><img alt="Research paper thumbnail of Nuclear calcium signaling: a cell within a cell" class="work-thumbnail" src="https://attachments.academia-assets.com/40290712/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/18852301/Nuclear_calcium_signaling_a_cell_within_a_cell">Nuclear calcium signaling: a cell within a cell</a></div><div class="wp-workCard_item"><span>Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.]</span><span>, 2009</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Calcium (Ca2+) is a versatile second messenger that regulates a wide range of cellular functions....</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Calcium (Ca2+) is a versatile second messenger that regulates a wide range of cellular functions. Although it is not established how a single second messenger coordinates diverse effects within a cell, there is increasing evidence that the spatial patterns of Ca2+ signals may determine their specificity. Ca2+ signaling patterns can vary in different regions of the cell and Ca2+ signals in nuclear and cytoplasmic compartments have been reported to occur independently. No general paradigm has been established yet to explain whether, how, or when Ca2+ signals are initiated within the nucleus or their function. Here we highlight that receptor tyrosine kinases rapidly translocate to the nucleus. Ca2+ signals that are induced by growth factors result from phosphatidylinositol 4,5-bisphosphate hydrolysis and inositol 1,4,5-trisphosphate formation within the nucleus rather than within the cytoplasm. This novel signaling mechanism may be responsible for growth factor effects on cell prolifer...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="533611c8f6a84f83d27aca234b2a00e7" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":40290712,"asset_id":18852301,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/40290712/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="18852301"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="18852301"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 18852301; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=18852301]").text(description); $(".js-view-count[data-work-id=18852301]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 18852301; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='18852301']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-a9bf3a2bc8c89fa2a77156577594264ee8a0f214d74241bc0fcd3f69f8d107ac.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "533611c8f6a84f83d27aca234b2a00e7" } } $('.js-work-strip[data-work-id=18852301]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":18852301,"title":"Nuclear calcium signaling: a cell within a cell","internal_url":"https://www.academia.edu/18852301/Nuclear_calcium_signaling_a_cell_within_a_cell","owner_id":38951595,"coauthors_can_edit":true,"owner":{"id":38951595,"first_name":"Dawidson","middle_initials":null,"last_name":"Gomes","page_name":"DawidsonGomes","domain_name":"ufmg","created_at":"2015-11-23T03:22:12.839-08:00","display_name":"Dawidson Gomes","url":"https://ufmg.academia.edu/DawidsonGomes"},"attachments":[{"id":40290712,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/40290712/thumbnails/1.jpg","file_name":"Nuclear_calcium_signaling_A_cell_within_20151123-20638-gpptza.pdf","download_url":"https://www.academia.edu/attachments/40290712/download_file","bulk_download_file_name":"Nuclear_calcium_signaling_a_cell_within.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/40290712/Nuclear_calcium_signaling_A_cell_within_20151123-20638-gpptza-libre.pdf?1448277987=\u0026response-content-disposition=attachment%3B+filename%3DNuclear_calcium_signaling_a_cell_within.pdf\u0026Expires=1739809940\u0026Signature=Wdxm6pJvNqU06gBeMyEXXX5MLllFWp-ei12CAE-ZFKNtzAB5nOJLkLlFcYVzsCbnqDcqLAGKRJkmU98c-IrQ9kU4jD4xmtuBXNeTlpSp5hMoTznq8d791nUoxoXqElDnHBvMAfr9JbHRuo8cAYrYKZkfEMgVNfXDHTi36LQ6J9AEjjgd2lAeKBF~7sK7a-pIgBStFdYd~MzcuezGJfGLS~QBZrYOKky6OF0~JUGGg6~acBCu~VIYXNciIIlsV2OZG36teuvfioRHSiH6NrN0AIG~gvwREQgAIeZxPgzlODrrqNe3eOTNfZjxqVAAZ-YjPAYZZBAbZN4sGZlK~DOOHQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}]}, dispatcherData: dispatcherData }); $(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="18852300"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/18852300/Effect_of_halothane_on_the_release_of_Ca2_i_in_dorsal_root_ganglion_neurons"><img alt="Research paper thumbnail of Effect of halothane on the release of [Ca2+]i in dorsal root ganglion neurons" class="work-thumbnail" src="https://attachments.academia-assets.com/40290702/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/18852300/Effect_of_halothane_on_the_release_of_Ca2_i_in_dorsal_root_ganglion_neurons">Effect of halothane on the release of [Ca2+]i in dorsal root ganglion neurons</a></div><div class="wp-workCard_item"><span>Neuroreport</span><span>, Jan 19, 2004</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">We investigated the effect of the volatile anaesthetic halothane on [Ca2+]i of dorsal root gangli...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">We investigated the effect of the volatile anaesthetic halothane on [Ca2+]i of dorsal root ganglion neurons. Halothane was able to increase [Ca2+]i in those neurons in a dose-dependent manner and independent of extracellular calcium. However, halothane action was inhibited by BAPTA-AM, suggesting the involvement of intracellular calcium stores. Dantrolene, an inhibitor of ryanodine-sensitive calcium stores had no effect while 2-APB, an inhibitor of IP3-sensitive calcium store reduced by 78% the halothane-evoked increase on [Ca2+]i. These data suggests that halothane increased [Ca2+]i of ganglion neurons through calcium release from IP3-sensitive calcium store. One possible consequence of the halothane action is to alter presynaptic activity and signaling pathways that influence neurotransmission.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="00927a4b7cf3e75085914766976d5c85" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":40290702,"asset_id":18852300,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/40290702/download_file?s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="18852300"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="18852300"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 18852300; 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