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RFP-Trap® Magnetic Particles M-270 | Proteintech
<!doctype html> <html lang="en"> <head> <meta charset="utf-8"> <meta http-equiv="X-UA-Compatible" content="IE=edge,chrome=1"> <title>RFP-Trap® Magnetic Particles M-270 | Proteintech</title> <meta name="viewport" content="user-scalable=no, width=device-width, initial-scale=1, maximum-scale=1"> <meta name="date" content="2025-02-15T01:02:49" /> <meta content="RFP-Trap® Magnetic Particles M-270 for immunoprecipitation (IP) of RFP-tagged proteins. RFP-Trap Magnetic Particles M-270 is highly recommended, when very large proteins/complexes are investigated, and magnetic separation is needed for IP. It consists of a RFP VHH/ Nanobody coupled to Magnetic Particles M-270. " name="description" /> <meta property="og:description" content="RFP-Trap® Magnetic Particles M-270 for immunoprecipitation (IP) of RFP-tagged proteins. RFP-Trap Magnetic Particles M-270 is highly recommended, when very large proteins/complexes are investigated, and magnetic separation is needed for IP. It consists of a RFP VHH/ Nanobody coupled to Magnetic Particles M-270. " /> <meta name="twitter:description" content="RFP-Trap® Magnetic Particles M-270 for immunoprecipitation (IP) of RFP-tagged proteins. RFP-Trap Magnetic Particles M-270 is highly recommended, when very large proteins/complexes are investigated, and magnetic separation is needed for IP. It consists of a RFP VHH/ Nanobody coupled to Magnetic Particles M-270. 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id="species-reactivity-Rabbit"> <label class="form-check-label" for="species-reactivity-Rabbit"> Rabbit </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="species-reactivity" value="Rat" id="species-reactivity-Rat"> <label class="form-check-label" for="species-reactivity-Rat"> Rat </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="species-reactivity" value="Sheep" id="species-reactivity-Sheep"> <label class="form-check-label" for="species-reactivity-Sheep"> Sheep </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="species-reactivity" value="Swine" id="species-reactivity-Swine"> <label class="form-check-label" for="species-reactivity-Swine"> Swine </label> </div> </li> </ul> <div class="search__dropdown__actions"> <button type="button" class="btn btn-sm btn-text search__dropdown__actions__clear">Clear</button> <button type="button" class="btn btn-sm btn-primary search__dropdown__actions__confirm">Confirm</button> </div> </div> </div> <div class="search__params" tabindex="0" data-search-field="species-reactivity"> <div class="search__params__container"> <div class="search-term-layout"> <div class="search-term-layout__term">Species Reactivity</div> <div class="search-term-layout__count">0</div> </div> </div> <div class="search__dropdown"> <ul class="list-unstyled m-0"> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="species-reactivity" value="Human" id="species-reactivity-Human"> <label class="form-check-label" for="species-reactivity-Human"> Human </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="species-reactivity" value="Mouse" id="species-reactivity-Mouse"> <label class="form-check-label" for="species-reactivity-Mouse"> Mouse </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="species-reactivity" value="Rat" id="species-reactivity-Rat"> <label class="form-check-label" for="species-reactivity-Rat"> Rat </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="species-reactivity" value="Pig" id="species-reactivity-Pig"> <label class="form-check-label" for="species-reactivity-Pig"> Pig </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="species-reactivity" value="Bovine" id="species-reactivity-Bovine"> <label class="form-check-label" for="species-reactivity-Bovine"> Bovine </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="species-reactivity" value="Chicken" id="species-reactivity-Chicken"> <label class="form-check-label" for="species-reactivity-Chicken"> Chicken </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="species-reactivity" value="Zebrafish" id="species-reactivity-Zebrafish"> <label class="form-check-label" for="species-reactivity-Zebrafish"> Zebrafish </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="species-reactivity" value="Hamster" id="species-reactivity-Hamster"> <label class="form-check-label" for="species-reactivity-Hamster"> Hamster </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="species-reactivity" value="Monkey" id="species-reactivity-Monkey"> <label class="form-check-label" for="species-reactivity-Monkey"> Monkey </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="species-reactivity" value="Drosophila" id="species-reactivity-Drosophila"> <label class="form-check-label" for="species-reactivity-Drosophila"> Drosophila </label> </div> </li> </ul> <div class="search__dropdown__actions"> <button type="button" class="btn btn-sm btn-text search__dropdown__actions__clear">Clear</button> <button type="button" class="btn btn-sm btn-primary search__dropdown__actions__confirm">Confirm</button> </div> </div> </div> <div class="search__params" tabindex="0" data-search-field="conjugate"> <div class="search__params__container"> <div class="search-term-layout"> <div class="search-term-layout__term">Conjugate</div> <div class="search-term-layout__count">0</div> </div> </div> <div class="search__dropdown"> <ul class="list-unstyled m-0"> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="conjugate" value="APC" id="conjugate-APC"> <label class="form-check-label" for="conjugate-APC"> APC </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="conjugate" value="Biotin" id="conjugate-Biotin"> <label class="form-check-label" for="conjugate-Biotin"> Biotin </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="conjugate" value="CoraLite®405" id="conjugate-CoraLite-405"> <label class="form-check-label" for="conjugate-CoraLite-405"> CoraLite®405 </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="conjugate" value="CoraLite®488" id="conjugate-CoraLite-488"> <label class="form-check-label" for="conjugate-CoraLite-488"> CoraLite®488 </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="conjugate" value="CoraLite®532" id="conjugate-CoraLite-532"> <label class="form-check-label" for="conjugate-CoraLite-532"> CoraLite®532 </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="conjugate" value="CoraLite®555" id="conjugate-CoraLite-555"> <label class="form-check-label" for="conjugate-CoraLite-555"> CoraLite®555 </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="conjugate" value="CoraLite®568" id="conjugate-CoraLite-568"> <label class="form-check-label" for="conjugate-CoraLite-568"> CoraLite®568 </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="conjugate" value="CoraLite®594" id="conjugate-CoraLite-594"> <label class="form-check-label" for="conjugate-CoraLite-594"> CoraLite®594 </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="conjugate" value="CoraLite®647" id="conjugate-CoraLite-647"> <label class="form-check-label" for="conjugate-CoraLite-647"> CoraLite®647 </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="conjugate" value="CoraLite®650" id="conjugate-CoraLite-650"> <label class="form-check-label" for="conjugate-CoraLite-650"> CoraLite®650 </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="conjugate" value="FITC" id="conjugate-FITC"> <label class="form-check-label" for="conjugate-FITC"> FITC </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="conjugate" value="HRP" id="conjugate-HRP"> <label class="form-check-label" for="conjugate-HRP"> HRP </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="conjugate" value="PE" id="conjugate-PE"> <label class="form-check-label" for="conjugate-PE"> PE </label> </div> </li> </ul> <div class="search__dropdown__actions"> <button type="button" class="btn btn-sm btn-text search__dropdown__actions__clear">Clear</button> <button type="button" class="btn btn-sm btn-primary search__dropdown__actions__confirm">Confirm</button> </div> </div> </div> <div class="search__params" tabindex="0" data-search-field="secondaryab-conjugate"> <div class="search__params__container"> <div class="search-term-layout"> <div class="search-term-layout__term">Conjugate</div> <div class="search-term-layout__count">0</div> </div> </div> <div class="search__dropdown"> <ul class="list-unstyled m-0"> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="conjugate" value="Alexa Fluor® 488" id="conjugate-alexa-fluor-488"> <label class="form-check-label" for="conjugate-Alexa-Fluor-488"> Alexa Fluor® 488 </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="conjugate" value="Alexa Fluor® 568" id="conjugate-alexa-fluor-568"> <label class="form-check-label" for="conjugate-Alexa-Fluor-568"> Alexa Fluor® 568 </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="conjugate" value="Alexa Fluor® 647" id="conjugate-alexa-fluor-647"> <label class="form-check-label" for="conjugate-Alexa-Fluor-647"> Alexa Fluor® 647 </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="conjugate" value="Alkaline phosphatase" id="conjugate-alkaline-phosphatase"> <label class="form-check-label" for="conjugate-Alkaline-phosphatase"> AP </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="conjugate" value="Biotin" id="conjugate-biotin"> <label class="form-check-label" for="conjugate-Biotin"> Biotin </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="conjugate" value="CoraLite®488" id="conjugate-CoraLite-488"> <label class="form-check-label" for="conjugate-CoraLite-488"> CoraLite®488 </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="conjugate" value="CoraLite®594" id="conjugate-CoraLite-594"> <label class="form-check-label" for="conjugate-CoraLite-594"> CoraLite®594 </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="conjugate" value="CoraLite®647" id="conjugate-CoraLite-647"> <label class="form-check-label" for="conjugate-CoraLite-647"> CoraLite®647 </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="conjugate" value="cy3" id="conjugate-cy3"> <label class="form-check-label" for="conjugate-Cy3"> Cy3 </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="conjugate" value="FITC" id="conjugate-FITC"> <label class="form-check-label" for="conjugate-FITC"> FITC </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="conjugate" value="HRP" id="conjugate-HRP"> <label class="form-check-label" for="conjugate-HRP"> HRP </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="conjugate" value="Rhodamine" id="conjugate-rhodamine"> <label class="form-check-label" for="conjugate-Rhodamine"> Rhodamine </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="conjugate" value="R-PE" id="conjugate-r-pe"> <label class="form-check-label" for="conjugate-R-PE"> R-PE </label> </div> </li> </ul> <div class="search__dropdown__actions"> <button type="button" class="btn btn-sm btn-text search__dropdown__actions__clear">Clear</button> <button type="button" class="btn btn-sm btn-primary search__dropdown__actions__confirm">Confirm</button> </div> </div> </div> <div class="search__params" tabindex="0" data-search-field="compatible-species"> <div class="search__params__container"> <div class="search-term-layout"> <div class="search-term-layout__term">Compatible Species</div> <div class="search-term-layout__count">0</div> </div> </div> <div class="search__dropdown"> <ul class="list-unstyled m-0"> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="species-reactivity" value="anti rabbit" id="compatible-species-Rabbit"> <label class="form-check-label" for="compatible-species-Rabbit"> Rabbit </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="species-reactivity" value="anti mouse" id="compatible-species-Mouse"> <label class="form-check-label" for="compatible-species-Mouse"> Mouse </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="species-reactivity" value="anti human" id="compatible-species-Human"> <label class="form-check-label" for="compatible-species-Human"> Human </label> </div> </li> </ul> <div class="search__dropdown__actions"> <button type="button" class="btn btn-sm btn-text search__dropdown__actions__clear">Clear</button> <button type="button" class="btn btn-sm btn-primary search__dropdown__actions__confirm">Confirm</button> </div> </div> </div> <div class="search__params" tabindex="0" data-search-field="flexable-conjugate"> <div class="search__params__container"> <div class="search-term-layout"> <div class="search-term-layout__term">Conjugate</div> <div class="search-term-layout__count">0</div> </div> </div> <div class="search__dropdown"> <ul class="list-unstyled m-0"> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="conjugate" value="CoraLite405" id="conjugate-CoraLite-Plus-405"> <label class="form-check-label" for="conjugate-CoraLite-Plus-405"> CoraLite Plus 405 </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="conjugate" value="FITC" id="conjugate-FITC"> <label class="form-check-label" for="conjugate-FITC"> FITC Plus<sup style="color:red"> NEW</sup> </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="conjugate" value="CoraLite488" id="conjugate-CoraLite-488"> <label class="form-check-label" for="conjugate-CoraLite-488"> CoraLite Plus 488 </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="conjugate" value="CoraLite555" id="conjugate-CoraLite-Plus-555"> <label class="form-check-label" for="conjugate-CoraLite-Plus-555"> CoraLite Plus 555 </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="conjugate" value="CoraLite647" id="conjugate-CoraLite-Plus-647"> <label class="form-check-label" for="conjugate-CoraLite-Plus-647"> CoraLite Plus 647 </label> </div> </li> <li class="search__dropdown__item"> <div class="form-check"> <input class="form-check-input" type="checkbox" name="conjugate" value="CoraLite750" id="conjugate-CoraLite-Plus-750"> <label class="form-check-label" for="conjugate-CoraLite-Plus-750"> CoraLite Plus 750 </label> </div> </li> </ul> <div class="search__dropdown__actions"> <button type="button" class="btn btn-sm btn-text search__dropdown__actions__clear">Clear</button> <button type="button" class="btn btn-sm btn-primary search__dropdown__actions__confirm">Confirm</button> </div> </div> </div> <div class="search__actions"> <button class="button" type="submit"> <svg xmlns="http://www.w3.org/2000/svg" width="16" height="16" fill="currentColor"> <path d="M11.742 10.344a6.5 6.5 0 1 0-1.397 1.398h-.001c.03.04.062.078.098.115l3.85 3.85a1 1 0 0 0 1.415-1.414l-3.85-3.85a1.007 1.007 0 0 0-.115-.1zM12 6.5a5.5 5.5 0 1 1-11 0 5.5 5.5 0 0 1 11 0z" /> </svg> <span>Search</span> </button> </div> </div> </form> </div> </div> </div> </section> <!-- MAIN --> <main data-page="product"> <div class="purchase-banner"> <div style="width: 80%; margin: 0 auto; line-height: 3.4375rem; "> <div class="row collapse"> <div class="small-12 medium-6 columns medium-offset-3"> <select data-prefix="Go To:"> <option data-href="#top">Overview</option> <option data-href="#product-information">Product Information</option> <option data-href="#product-documentation">Documentation</option> <option data-href="#publications">Publications (6)</option> <option data-href="#reviews">Reviews (1)</option> </select> </div> </div> </div> </div> <div class="row small-uncollapse xlarge-collapse"> <div class="medium-9 small-12 columns" data-section="hero"> <section class="row collapse" data-equalizer data-equalizer-mq="medium-up"> <div class="small-12 medium-12 columns" data-equalizer-watch> <div class="product-title"> <h1>ChromoTek RFP-Trap® Magnetic Particles M-270</h1> <h5 class="grey3"> RFP-Trap® Magnetic Particles M-270 for immunoprecipitation (IP) of RFP-tagged proteins. RFP-Trap Magnetic Particles M-270 is highly recommended, when very large proteins/complexes are investigated, and magnetic separation is needed for IP. It consists of a RFP VHH/ Nanobody coupled to Magnetic Particles M-270. </h5> </div> <div class="product-validation"> <!-- Dev note: if a ecito here has no data then dont create the div section --> <div><p class="grey1 m-0"><strong>Specificity</strong></p><p>mCherry, mPlum, mRFP</p></div><div><p class="grey1 m-0"><strong>Applications</strong></p><p>IP, CoIP</p></div><div><p class="grey1 m-0"><strong>Conjugate</strong></p><p>Magnetic Particles M-270</p></div><div><p class="grey1 m-0"><strong>Type</strong></p><p>Nanobody</p></div> <div><p class="grey1 m-0"><strong><a href="#publications">Publications</a></strong></p><p>6</p></div> <div><p class="grey1 m-0"><strong><a href="#reviews">Reviews (1)</a></strong></p><ul class="star-rating" title="3/5 rating"><i class="fa fa-star" aria-hidden="true"></i><i class="fa fa-star" aria-hidden="true"></i><i class="fa fa-star" aria-hidden="true"></i><i class="fa fa-star disabled" aria-hidden="true"></i><i class="fa fa-star disabled" aria-hidden="true"></i></ul></div> </div> </div> </section> </div> <div class="medium-3 small-12 columns" data-section="hero"> <section class="row collapse product-extra" data-equalizer data-equalizer-mq="medium-up"> <p class="hide-for-small"><a href="/products/chromotek-nanobody-based-reagents/"><img src="/img/logos/Logo_Chromotek_Menu.png" alt="" style="width:180px; "></a></p> <div class="small-12 medium-12 columns" data-equalizer-watch> <div class=""> <p><strong class="green">Cat no :</strong> <span id="spnCatNo" data-maintype="Nanobody" data-producttype="Nano-Trap" data-source="CTK" data-issellable="True">rtd</span></p> <p class="green m-0"><strong>Synonyms</strong></p> <p style="overflow: hidden;hyphens: auto;display: -webkit-box;-webkit-box-orient: vertical;-webkit-line-clamp: 3;"> Magnetic Particles M-270, RFP Magnetic Particles M-270, RFP tag, RFP-tag, RFP-Trap® , RFP-Trap® Magnetic Particles M-270, rtd, rtd-10, rtd-100, rtd-20, rtd-200, rtd-400</p> </div> </div> </section> </div> </div> <hr class="mb-40" /> <div class="row small-uncollapse xlarge-collapse" data-reorder="product"> <nav class="small-12 medium-2 columns" data-reorder="links"> <section class="product-inpagelinks"> <div id="inpage-links"> <div class="hide-for-small"> <p><a href="#top" class="green"><strong>Overview</strong></a></p> <hr /> <ul> <li><a href="#product-information">Product Information</a></li> <li><a href="#product-documentation">Documentation</a></li> <li><a href="#publications">Publications (6)</a></li> <li><a href="#reviews">Reviews (1)</a></li> </ul> <hr /> </div> </div> </section> </nav> <div class="small-12 medium-7 columns col-spacer" data-reorder="gallery"> <section class="product-gallery"> <h3>Validation Data Gallery</h3> <div class="image-slider"> <ul data-orbit data-options="timer: false; slide_number: false;" style="min-height:330px;"><li><div class="image-slider-item"><div class="image-slider-image" style="line-height: 330px;"><a data-reveal-id="resultImageModal-1" class="link"><img src="/products/pictures/rtd-1.jpg" alt="Immunoprecipitation (IP) of mCherry with RFP-Trap Magnetic Particles M-270. I: Input, FT: Flow-Through, B: Bound" title="Immunoprecipitation (IP) of mCherry with RFP-Trap Magnetic Particles M-270. I: Input, FT: Flow-Through, B: Bound" style='display: inline-block;vertical-align: middle;max-height: 312px;width: auto;' /></a></div><div class="image-slider-body"><div class="image-slider-text"><p style="max-height: 144px; overflow: hidden; hyphens: auto; display: -webkit-box; -webkit-box-orient: vertical; -webkit-line-clamp: 8; ">Immunoprecipitation (IP) of mCherry with RFP-Trap Magnetic Particles M-270. I: Input, FT: Flow-Through, B: Bound</p></div><button class="btn-link" data-reveal-id="galleryModal">View All Images (3)</button></div></div><section class="reveal-modal small text-center bg-white" id="resultImageModal-1" data-reveal aria-hidden="true" role="dialog"><a class="close-reveal-modal">×</a><p>Immunoprecipitation (IP) of mCherry with RFP-Trap Magnetic Particles M-270. I: Input, FT: Flow-Through, B: Bound</p><img src="/products/pictures/rtd-1.jpg" alt="" style="max-height: 500px;" /></section></li><li><div class="image-slider-item"><div class="image-slider-image" style="line-height: 330px;"><a data-reveal-id="resultImageModal-2" class="link"><img src="/products/pictures/rtd-2.jpg" alt="Coomassie and Western blot to show the effectivity of immunoprecipitation (IP) of mCherry with RFP-Trap Magnetic Particles M-270. I: Input, FT: Flow-Through, B: Bound" title="Coomassie and Western blot to show the effectivity of immunoprecipitation (IP) of mCherry with RFP-Trap Magnetic Particles M-270. I: Input, FT: Flow-Through, B: Bound" style='display: inline-block;vertical-align: middle;max-height: 312px;width: auto;' /></a></div><div class="image-slider-body"><div class="image-slider-text"><p style="max-height: 144px; overflow: hidden; hyphens: auto; display: -webkit-box; -webkit-box-orient: vertical; -webkit-line-clamp: 8; ">Coomassie and Western blot to show the effectivity of immunoprecipitation (IP) of mCherry with RFP-Trap Magnetic Particles M-270. I: Input, FT: Flow-Through, B: Bound</p></div><button class="btn-link" data-reveal-id="galleryModal">View All Images (3)</button></div></div><section class="reveal-modal small text-center bg-white" id="resultImageModal-2" data-reveal aria-hidden="true" role="dialog"><a class="close-reveal-modal">×</a><p>Coomassie and Western blot to show the effectivity of immunoprecipitation (IP) of mCherry with RFP-Trap Magnetic Particles M-270. I: Input, FT: Flow-Through, B: Bound</p><img src="/products/pictures/rtd-2.jpg" alt="" style="max-height: 500px;" /></section></li><li><div class="image-slider-item"><div class="image-slider-image" style="line-height: 330px;"><a data-reveal-id="resultImageModal-3" class="link"><img src="/products/pictures/rtd-4.jpg" alt="Cartoon to visualize the binding of large GFP-fusion proteins (GFP (green) + protein of interest (POI, blue) with interacting partner X (Prot X, orange) or multimeric proteins (GFP (green) + protein of interest (POI, blue) to the GFP VHH (dark green) of GFP-Trap Magnetic Agarose or GFP-Trap Magnetic Particles M-270." title="Cartoon to visualize the binding of large GFP-fusion proteins (GFP (green) + protein of interest (POI, blue) with interacting partner X (Prot X, orange) or multimeric proteins (GFP (green) + protein of interest (POI, blue) to the GFP VHH (dark green) of GFP-Trap Magnetic Agarose or GFP-Trap Magnetic Particles M-270." style='display: inline-block;vertical-align: middle;max-height: 312px;width: auto;' /></a></div><div class="image-slider-body"><div class="image-slider-text"><p style="max-height: 144px; overflow: hidden; hyphens: auto; display: -webkit-box; -webkit-box-orient: vertical; -webkit-line-clamp: 8; ">Cartoon to visualize the binding of large GFP-fusion proteins (GFP (green) + protein of interest (POI, blue) with interacting partner X (Prot X, orange) or multimeric proteins (GFP (green) + protein of interest (POI, blue) to the GFP VHH (dark green) of GFP-Trap Magnetic Agarose or GFP-Trap Magnetic Particles M-270.</p></div><button class="btn-link" data-reveal-id="galleryModal">View All Images (3)</button></div></div><section class="reveal-modal small text-center bg-white" id="resultImageModal-3" data-reveal aria-hidden="true" role="dialog"><a class="close-reveal-modal">×</a><p>Cartoon to visualize the binding of large GFP-fusion proteins (GFP (green) + protein of interest (POI, blue) with interacting partner X (Prot X, orange) or multimeric proteins (GFP (green) + protein of interest (POI, blue) to the GFP VHH (dark green) of GFP-Trap Magnetic Agarose or GFP-Trap Magnetic Particles M-270.</p><img src="/products/pictures/rtd-4.jpg" alt="" style="max-height: 500px;" /></section></li></ul> </div> </section> </div> <aside class="small-12 medium-3 columns mb-5" data-reorder="pricing"> <section class="product-pricing" id="purchase"> <form> <div class="row collapse"> <div class="small-12 columns"> <div class="mb-20"> <h3 class="price"> <span class="price-amount">-</span>/ <span class="price-size">-</span> </h3> <p class="grey1"><strong>Cat No.</strong> rtd</p> <p class="grey1 NextDayDelivery"> </p> <p class="grey1"><strong>Freight/Packing:</strong> <span class="green" id="spFreightFee">-</span></p> </div> </div> </div> <div class="row collapse"> <div class="small-12 columns"> <label class="m-0">Country/Region</label> <select class="small mb-20" name="country" id="drpCountry"> <option 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RFP-Trap Magnetic Particles M-270 is highly recommended, when very large proteins/complexes are investigated, and magnetic separation is needed for IP. It consists of a RFP VHH/ Nanobody coupled to Magnetic Particles M-270. </p> <table role="grid" class="mb-40 vert-top"> <tbody> <tr><td class="subtitle">Description</td><td>Immunoprecipitation of RFP-fusion proteins and their interacting factors with anti-RFP Nanobody conjugated to magnetic particles. RFP-Trap Magnetic Particles M-270 is recommended when very large proteins/complexes are investigated and magnetic separation is needed for IP. <p style="margin-left: 25px;"> • IP and Co-IP of RFP-tagged proteins without size limitation </p><p style="margin-left: 25px;"> • Automation and high throughput applications </p><p style="margin-left: 25px;"> • Magnetic separation with easy and efficient washing of RFP-Trap Magnetic Particles M-270 </p><p style="margin-left: 25px;"> • Very high affinity (KD=5 nM) to bind even low abundant proteins </p><p style="margin-left: 25px;"> • No heavy & light antibody chains, short incubation (5-30 min) </p><p style="margin-left: 25px;"> • Extraordinary binding, also under harsh conditions</p> </td></tr><tr><td class="subtitle">Applications</td><td>IP, CoIP, ChIP, RIP</td></tr><tr><td class="subtitle">Specificity/Target</td><td>mRFP, mCherry, mRFPruby, mPlum, tagRFP, mKate2, mOrange, PA-mCherry, mScarlet <br>For the complete list, please click here: <a class="green" href="/products/pictures/pdf/Fluorescent_Specificity_by_Nano-Trap_190222.pdf" target="_blank" rel="nofollow">Fluorescent protein specificity table</a></td></tr><tr><td class="subtitle">Binding capacity</td><td>1.25 μg of recombinant mCherry per 25 μL bead slurry</td></tr><tr><td class="subtitle">Conjugate</td><td>Magnetic Particles M-270, size: 2.8 µm<br>high throughput-compatible</td></tr><tr><td class="subtitle">Elution buffer</td><td>SDS sample buffer<br>0.2 M glycine pH 2.5</td></tr><tr><td class="subtitle">Wash buffer compatibility</td><td>0.5 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 2 % Triton X-100</td></tr><tr><td class="subtitle">Type </td><td>Nanobody</td></tr><tr><td class="subtitle">Class</td><td>Recombinant</td></tr><tr><td class="subtitle">Host</td><td>Alpaca</td></tr><tr><td class="subtitle">Affinity (K<sub>D</sub>) </td><td>Dissociation constant K<sub>D</sub> of 5 nM</td></tr><tr><td class="subtitle">Compatibility with mass spectrometry</td><td>The RFP-Trap® is optimized for on-bead digestion. For the application note, please click here: <a class="green" href="/products/pictures/pdf/App_note_ChromoTek_on_bead_digest_protocol.pdf" target="_blank" rel="nofollow">On-bead digest protocol for mass spectrometry</a></td></tr><tr><td class="subtitle">RRID</td><td>AB_2861253</td></tr><tr><td class="subtitle">Storage Buffer</td><td>PBS with 0.09% sodium azide</td></tr><tr><td class="subtitle">Storage Condition</td><td>Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!</td></tr><tr><td class="subtitle">Size</td><td>25ul/reactions (eg:20rxns=500ul slurry)</td></tr> </tbody> </table> </div> </div> </section> </div> <div class="row collapse pt-60" id="product-documentation" data-section="product-documentation"><section class="product-documentation"><div class="row"><div class="small-12 medium-7 medium-offset-2 columns"><h3>Documentation</h3><table role="grid" class="sm-block"><thead><tr><th>SDS</th></tr></thead><tbody><tr><td class="subtitle blue"><a class="green" href="/products/pictures/pdf/rtd_SDS_RFP-Trap__Magnetic_Particles_M-270__EN_.pdf" target="_blank" rel="nofollow">SDS RFP-Trap Magnetic Particles M-270</a></td></tr></tbody></table><table role="grid" class="sm-block"><thead><tr><th>RFP-Trap Specificity</th></tr></thead><tbody><tr><td class="subtitle blue"><a class="green" href="/products/pictures/pdf/Fluorescent_Specificity_by_Nano-Trap_220819.pdf" target="_blank" rel="nofollow">Fluorescent protein specificity table</a></td></tr></tbody></table><table role="grid" class="sm-block"><thead><tr><th>PROTOCOL</th></tr></thead><tbody><tr><td class="subtitle blue"><a class="green" href="/products/pictures/pdf/rtd_Manual_RFP-Trap__Magnetic_Particles_M-270_.pdf" target="_blank" rel="nofollow">PROTOCOL RFP-Trap Magnetic Particles M-270</a></td></tr></tbody></table><table role="grid" class="sm-block"><thead><tr><th>Trouble shooting</th></tr></thead><tbody><tr><td class="subtitle blue"><a class="green" href="/products/pictures/pdf/Troubleshooting_guide_Nano-Traps.pdf" target="_blank" rel="nofollow">Troubleshooting guide immunoprecipitation (IP)</a></td></tr></tbody></table></div></div></section></div> <div class="row collapse pt-60" id="product-otherformat" data-section="product-otherformat"><section class="product-otherformat"><div class="row"><div class="small-12 medium-7 medium-offset-2 columns"><h3>Other Formats</h3><table role="grid" class="sm-block"><tbody><tr><td class="subtitle blue"><a class="green" href="/products/RFP-Trap-Agarose-kit-rtak.htm" target="_blank" rel="nofollow">RFP-Trap Agarose kit</a></td></tr><tr><td class="subtitle blue"><a class="green" href="/products/RFP-Trap-Agarose-rta.htm" target="_blank" rel="nofollow">RFP-Trap Agarose</a></td></tr><tr><td class="subtitle blue"><a class="green" href="/products/RFP-Trap-Magnetic-Agarose-kit-rtmak.htm" target="_blank" rel="nofollow">RFP-Trap Magnetic Agarose kit</a></td></tr><tr><td class="subtitle blue"><a class="green" href="/products/RFP-Trap-Magnetic-Agarose-rtma.htm" target="_blank" rel="nofollow">RFP-Trap Magnetic Agarose</a></td></tr><tr><td class="subtitle blue"><a class="green" href="/products/RFP-Trap-Magnetic-Particles-M-270-Kit-rtdk.htm" target="_blank" rel="nofollow">RFP-Trap Magnetic Particles M-270 Kit</a></td></tr></tbody></table></div></div></section></div> </div> </div> <div> <div class="pb-40"> <section class="publications pt-60" id="publications"> <div class="row"> <div class="small-12 medium-7 medium-offset-2 columns"> <h3>Publications</h3><div class="type-categories flex-justify-between mb-20"><ul class="inline-list mb-0"><li><a href="" data-category="">All (6)</a></li><li><a href="" data-category="WB">WB (2)</a></li><li><a href="" data-category="IP">IP (2)</a></li><li><a href="" data-category="CoIP">CoIP (1)</a></li><li><a href="" data-category="ChIP">ChIP (1)</a></li></ul><select class="small" name="publications-sortby" id="publications-sortby" data-prefix="Sort By:"><option value="ImpactFactor">Journal Impact Factor</option><option value="PubDate">Most recent</option></select></div><table role="grid" class="mb-40 no-stripes vert-top"><thead><tr><th width="16%">Application</th><th>Title</th></tr></thead><tbody id="publications-ajax" class="ajax-panel"><tr><td class="hide-for-small">ChIP</td><td><div><p><strong>Plant Cell</strong></p><h4>Diversification of heat shock transcription factors expanded thermal stress responses during early plant evolution.</h4><blockquote><footer>Authors - Ting-Ying Wu</footer></blockquote><div class="flex"><a href="https://www.ncbi.nlm.nih.gov/pubmed/35849348?dopt=Abstract" class="green" target="_blank" rel="nofollow">View Article</a></div></div></td></tr><tr><td class="hide-for-small">IP</td><td><div><p><strong>Cell Rep</strong></p><h4>RNA-binding protein FXR1 drives cMYC translation by recruiting eIF4F complex to the translation start site.</h4><blockquote><footer>Authors - Jasmine George</footer></blockquote><div class="flex"><a href="https://www.ncbi.nlm.nih.gov/pubmed/34731628?dopt=Abstract" class="green" target="_blank" rel="nofollow">View Article</a></div></div></td></tr><tr><td class="hide-for-small">WB</td><td><div><p><strong>New Phytol</strong></p><h4>Increasing amyloplast size in wheat endosperm through mutation of PARC6 affects starch granule morphology</h4><blockquote><footer>Authors - Lara Esch</footer></blockquote><div class="flex"><a href="https://www.ncbi.nlm.nih.gov/pubmed/37424336?dopt=Abstract" class="green" target="_blank" rel="nofollow">View Article</a></div></div></td></tr><tr><td class="hide-for-small">CoIP</td><td><div><p><strong>Mol Biol Cell</strong></p><h4>FARL-11 (STRIP1/2) is required for sarcomere and sarcoplasmic reticulum organization in C. elegans</h4><blockquote><footer>Authors - Sterling C T Martin</footer></blockquote><div class="flex"><a href="https://www.ncbi.nlm.nih.gov/pubmed/37314837?dopt=Abstract" class="green" target="_blank" rel="nofollow">View Article</a></div></div></td></tr><tr><td class="hide-for-small">IP</td><td><div><p><strong>Nat Commun</strong></p><h4>PARP2 promotes Break Induced Replication-mediated telomere fragility in response to replication stress</h4><blockquote><footer>Authors - Daniela Muoio</footer></blockquote><div class="flex"><a href="https://www.ncbi.nlm.nih.gov/pubmed/38565848?dopt=Abstract" class="green" target="_blank" rel="nofollow">View Article</a></div></div></td></tr><tr><td class="hide-for-small">WB</td><td><div><p><strong>Mol Cell</strong></p><h4>Alphavirus infection triggers selective cytoplasmic translocation of nuclear RBPs with moonlighting antiviral roles</h4><blockquote><footer>Authors - Wael Kamel</footer></blockquote><div class="flex"><a href="https://www.ncbi.nlm.nih.gov/pubmed/39642884?dopt=Abstract" class="green" target="_blank" rel="nofollow">View Article</a></div></div></td></tr></tbody></table> </div> </div> </section> </div> <div class="bgColor-wrapper"> <div class="pb-40"> <section class="reviews pt-60" id="reviews"> <div class="row"><div class="small-12 medium-7 medium-offset-2 columns"><h3>Reviews</h3><ul class="star-rating mb-20" title="3/5 rating"><i class="fa fa-star" aria-hidden="true"></i><i class="fa fa-star" aria-hidden="true"></i><i class="fa fa-star" aria-hidden="true"></i><i class="fa fa-star disabled" aria-hidden="true"></i><i class="fa fa-star disabled" aria-hidden="true"></i><strong style="margin-left:5px;"><a href="/news/blog/proteintech-product-reviews/?catNo=rtd" target="_blank" class="add-review link" rel="nofollow">Add a review</a></strong></ul><p>The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.</p><hr class="hide-for-small" /><div class="reviews-wrapper"><table role="grid" class="mb-0 no-stripes vert-top sm-block"><tbody id="reviews-ajax" class="ajax-panel"><tr><td><div class="review-header"><div class="avatar mr-20">FH</div><div><p class="m-0">Sreejith (Verified Customer) (11-19-2024)</p><ul class="star-rating" title="3/5 rating"><i class="fa fa-star" aria-hidden="true"></i><i class="fa fa-star" aria-hidden="true"></i><i class="fa fa-star" aria-hidden="true"></i><i class="fa fa-star disabled" aria-hidden="true"></i><i class="fa fa-star disabled" aria-hidden="true"></i></ul></div></div></td><td><div><h4></h4><p>very faint band corresponding to the size of the protein of interest.</p><ul class="mb-20"><li><strong>Applications:</strong> Immunoprecipitation</li><li><strong>Cell Tissue Type:</strong> Physcomitrella patens</li></ul></div></td></tr></tbody></table></div></div></div> </section> </div> </div> </div> </main> <!-- MAIN --> <div hidden data-section="gallery"> <section class="gallery reveal-modal full" id="galleryModal" data-reveal aria-hidden="true" role="dialog"> <a class="close-reveal-modal">×</a> <div class="row"> <div class="small-12 medium-10 medium-centered columns"> <div class="row"><div class="small-12 columns"></div><div class="small-12 medium-4 columns end"><div style="height:303px;text-align:center;"><img src="/products/pictures/rtd-1.jpg" style='display: inline-block;vertical-align: middle;max-height: 300px;' /></div><p style="height: 90px; overflow: auto;">Immunoprecipitation (IP) of mCherry with RFP-Trap Magnetic Particles M-270. I: Input, FT: Flow-Through, B: Bound</p><hr /></div><div class="small-12 medium-4 columns end"><div style="height:303px;text-align:center;"><img src="/products/pictures/rtd-2.jpg" style='display: inline-block;vertical-align: middle;max-height: 300px;' /></div><p style="height: 90px; overflow: auto;">Coomassie and Western blot to show the effectivity of immunoprecipitation (IP) of mCherry with RFP-Trap Magnetic Particles M-270. I: Input, FT: Flow-Through, B: Bound</p><hr /></div><div class="small-12 medium-4 columns end"><div style="height:303px;text-align:center;"><img src="/products/pictures/rtd-4.jpg" style='display: inline-block;vertical-align: middle;max-height: 300px;' /></div><p style="height: 90px; overflow: auto;">Cartoon to visualize the binding of large GFP-fusion proteins (GFP (green) + protein of interest (POI, blue) with interacting partner X (Prot X, orange) or multimeric proteins (GFP (green) + protein of interest (POI, blue) to the GFP VHH (dark green) of GFP-Trap Magnetic Agarose or GFP-Trap Magnetic Particles M-270.</p><hr /></div></div> </div> </div> </section> </div> <section class="reveal-modal tiny text-center bg-white" id="inquireModal" data-reveal aria-hidden="true" role="dialog" style="padding:10px 0px;min-width:450px;"> <a class="close-reveal-modal">×</a> <iframe src="https://go.ptglab.com/l/685213/2020-12-13/fv4tc" type="text/html" frameborder="0" id="sizetracker" width="100%" style="min-height:800px;"> Sorry, your browser does not support IFRAMES </iframe> </section> <div class="to-top"> <a href="#top"> <svg class="svg-uparrow" viewBox="0 0 50 50" version="1.1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"> <g stroke="none" stroke-width="1" fill-rule="evenodd"> <path d="M46.9706009,24.8878112 C46.9937002,24.9862271 47,24.9926456 47,25.0012035 C46.9632511,25.4601211 46.8183554,25.8013676 46.5821123,26.0666626 C46.5285639,26.1650785 46.4960149,26.1565206 46.4750155,26.1747062 C46.4540161,26.1939614 29.1893762,42.142687 29.1893762,42.142687 C28.5908939,42.7524377 27.6186226,42.7524377 27.0201403,42.142687 C26.420608,41.5318665 26.420608,40.5444982 27.0201403,39.9347475 L41.4194149,26.5983225 L4.57075359,26.5983225 C3.70347922,26.5983225 3,25.8815979 3,25.0001337 C3,24.1175998 3.70347922,23.3998055 4.57075359,23.3998055 L41.4194149,23.3998055 L27.0201403,10.0665897 C26.420608,9.45576922 26.420608,8.4673312 27.0201403,7.85651074 C27.6186226,7.24782975 28.5908939,7.24782975 29.1893762,7.85651074 L46.3122703,23.7132388 C46.4288169,23.8234218 46.5033647,23.806306 46.5516632,23.8555139 C46.6115115,23.9186285 46.594712,24.0084865 46.6419606,24.0769497 C46.8225552,24.3090829 46.9464516,24.5743779 46.9706009,24.8878112 Z" fill="#FFFFFF" transform="translate(25.000000, 25.000000) rotate(-90.000000) translate(-25.000000, -25.000000) "> </path> </g> </svg> </a> </div> <footer> <div class="row collapse"> <div class="small-12 medium-12 medium-centered columns"> <div class="row address show-for-medium-up"> <ul class="small-block-grid-2 medium-block-grid-4"> <li> <h6>Proteintech North America (HQ)</h6> <hr class="small"> <address> Proteintech Group, Inc<br> 5500 Pearl Street, Suite 400<br> Rosemont, IL 60018, USA<br> </address> <p> <a href="tel:1-888-478-4522">1-888-478-4522</a><br> <a href="mailto:proteintech@ptglab.com">proteintech@ptglab.com</a> </p> </li> <li> <h6>Proteintech Europe</h6> <hr class="small"> <address> Transmission (6th Fl)<br> 6 Atherton Street<br> M3 3GS, Manchester, UK<br> </address> <p> <a href="tel:(+44) 161 839 3007">(+44) 161 839 3007</a><br> <a href="mailto:europe@ptglab.com">europe@ptglab.com</a> </p> </li> <li> <h6>ChromoTek & Proteintech Germany</h6> <hr class="small"> <address> Fraunhoferstr. 1<br> 82152, Planegg-Martinsried<br> Germany<br> </address> <p> <a href="tel:+49 89 124 148 850">+49 89 124 148 850</a><br> <a href="mailto:germany@ptglab.com">germany@ptglab.com</a> </p> </li> <li class="hide-for-medium"> <img src="/img/logo_grey.png"> </li> </ul> <hr /> </div> <div class="row"> <div class="social"> <ul class="inline-list"> <li><a href="http://www.youtube.com/proteintech"><i class="fa fa-youtube-play fa-2x"></i></a></li> <li> <a href="https://www.linkedin.com/company/proteintech-group/"> <i class="fa fa-linkedin-square fa-2x"></i> </a> </li> <li><a href="http://www.twitter.com/proteintech"><i class="fa fa-twitter fa-2x"></i></a></li> <li> <a href="https://www.facebook.com/Proteintech-311515338877884/timeline/"> <i class="fa fa-facebook-square fa-2x"></i> </a> </li> <li><a href="https://www.instagram.com/proteintech/"><i class="fa fa-instagram fa-2x"></i></a></li> </ul> </div> <div class="privacy"> <p> <a class="show-for-small-only" href="/contact">Contact Us</a> <a href="https://www.ptglab.com/news/blog/privacy-policy/" target="_blank">Privacy Policy</a> <a href="/terms-of-use">Terms of use</a> <a href="/news/blog/product-terms/" target="_blank">Terms and Conditions</a> <a href="/trademark-information/" target="_blank">Trademark Information</a> <a href="/imprint-impressum/" target="_blank">Imprint (Impressum)</a> <a href="/modern-slavery-statement/" target="_blank" style="padding: 0;"> Modern Slavery Statement </a> </p> <p> <a href="/chromotek-privacy-policy/">ChromoTek GmbH Privacy Policy</a> <a href="#">Copyright © 2002-2022 Proteintech Group, Inc. 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