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</ul></nav></div></section> </div><div class="breadcrumb"><span class="breadcrumb-link-wrap"><a href="https://www.thermofisher.com/blog/?">Accelerating Science</a></span><span class="breadcrumb-link-wrap"><a class="breadcrumb-link" href="https://www.thermofisher.com/blog/learning-at-the-bench/"><span class="breadcrumb-link-text-wrap">Learning at the Bench</span></a><meta ></span> <span aria-label="breadcrumb separator">/</span> <span class="breadcrumb-link-wrap"><a class="breadcrumb-link" href="https://www.thermofisher.com/blog/learning-at-the-bench/cell/"><span class="breadcrumb-link-text-wrap">細胞培養・イメージング</span></a><meta ></span> <span aria-label="breadcrumb separator">/</span> <span class="breadcrumb-current-title">低コストで浮遊細胞の大量培養を可能に！CO₂インキュベーター用新シェルフシステム</span></div></div></header><div class="site-inner"><div class="content-sidebar-wrap"><main class="content"><article class="post-16810 post type-post status-publish format-standard has-post-thumbnail category-cell tag-laboratory tag-cell-culture entry" aria-label="低コストで浮遊細胞の大量培養を可能に！CO₂インキュベーター用新シェルフシステム"><header class="entry-header"><h1 class="entry-title">低コストで浮遊細胞の大量培養を可能に！CO₂インキュベーター用新シェルフシステム</h1> <span class="post-info-author"> 作成者 <a href="https://www.thermofisher.com/blog/author/lgbadmin/" title="LatB Staff の投稿" rel="author">LatB Staff</a> </span> <span class="post-info-date"> 04.05.2024 </span> </header><div class="entry-content"><p>近年、CAR-T細胞療法をはじめとする遺伝子細胞治療分野の発展に伴い、免疫細胞を安全かつ大量に培養するニーズが増えています。このため、細胞の汚染リスクを低減しつつ大量培養を実現するための技術や製品が求められるようになり、多くのメーカーにより自動化培養装置やリアクターの開発が進められてきました。その一方で、最新の自動化装置やリアクターは、装置自体が大型で高額な場合も多く、導入が進まないなどの課題も残されていました。<br /> 本ブログでは、既存の大型CO<sub>2</sub>インキュベーターを使用して、低コストで免疫細胞をはじめとする非接着細胞の大量培養を実現できる新しいシェルフシステムをご紹介いたします。</p> <div id="toc_container" class="no_bullets"><p class="toc_title">▼もくじ</p><ul class="toc_list"><li><a href="#CO2">拡大培養に適した大容量CO2インキュベーターとは</a></li><li><a href="#i">閉鎖系で非接着細胞の拡大培養が可能なバイオリアクターの登場</a></li><li><a href="#25">低コストで非接着細胞の生産性を2.5倍に増強する新しいシェルフシステム</a></li><li><a href="#i-2">まとめ</a><ul><li><a href="#G-RexCultiMaxx">G-Rex™バイオリアクター向けのCultiMaxxシステム</a></li></ul></li></ul></div> <h2><span id="CO2">拡大培養に適した大容量CO<sub>2</sub>インキュベーターとは</span></h2> <p>当社はライフサイエンスやヘルスケア分野のお客さまにCO₂インキュベーターを供給してきました。その中でも、Thermo Scientific™ Forma™ Steri-Cycle™ i250 CO<sub>2</sub>インキュベーターおよびi250 CR CO<sub>2</sub>インキュベーターCTS™ シリーズは、その信頼性、傑出した汚染防止機能、培養細胞に適した増殖条件、そしてクリーンルームへの適合性などが高く評価され、世界中の遺伝子細胞治療分野の細胞加工施設で多く採用されています。</p> <p><iframe title="Introducing Heracell™ Vios™ 160i CR certified cleanroom compatible CO2 Incubator" width="760" height="428" src="https://www.youtube.com/embed/kYsX8DtPxf0?feature=oembed" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" referrerpolicy="strict-origin-when-cross-origin" allowfullscreen></iframe></p> <p><b>Forma Steri-Cycle i250 CO<sub>2</sub>インキュベーター／i250 CR CO<sub>2</sub>インキュベーターCTSシリーズの特長</b></p> <ul> <li>255 Lの大容量チャンバーで細胞の拡大培養に適した大容量モデル</li> <li>Forma Steri-Cycle i250 CR CO<sub>2</sub>インキュベーターCTSシリーズはISO Class 5、GMP Grade A/Bクリーンルームへの適合認証取得</li> <li>Thermo Scientific™ THRIVE™ アクティブエアフローテクノロジーによる優れた環境復帰性能</li> <li>チャンバー内HEPAフィルターによりISO Class 5レベルのクリーンエアーを維持し汚染を防止</li> <li>Thermo Scientific™ Steri-Run™ 乾熱滅菌機能を装備し180 ℃で12時間以内に滅菌が完了</li> <li>酸素制御をオプションで追加可能</li> <li>OPC-UA™ によるデータ通信が可能（オプション)</li> </ul> <div id="attachment_16825" style="width: 542px" class="wp-caption aligncenter"><img decoding="async" aria-describedby="caption-attachment-16825" src="https://www.thermofisher.com/blog/learning-at-the-bench/wp-content/uploads/sites/13/2024/04/d1c1c6088cd83b39003df14c4dceb701-943x1024.png" alt="Forma Steri-Cycle i250 CR CO2インキュベーター" width="532" height="577" class="size-large wp-image-16825" srcset="https://www.thermofisher.com/blog/learning-at-the-bench/wp-content/uploads/sites/13/2024/04/d1c1c6088cd83b39003df14c4dceb701-943x1024.png 943w, https://www.thermofisher.com/blog/learning-at-the-bench/wp-content/uploads/sites/13/2024/04/d1c1c6088cd83b39003df14c4dceb701-276x300.png 276w, https://www.thermofisher.com/blog/learning-at-the-bench/wp-content/uploads/sites/13/2024/04/d1c1c6088cd83b39003df14c4dceb701-768x834.png 768w, https://www.thermofisher.com/blog/learning-at-the-bench/wp-content/uploads/sites/13/2024/04/d1c1c6088cd83b39003df14c4dceb701-1415x1536.png 1415w, https://www.thermofisher.com/blog/learning-at-the-bench/wp-content/uploads/sites/13/2024/04/d1c1c6088cd83b39003df14c4dceb701.png 1725w" sizes="(max-width: 532px) 100vw, 532px" /><p id="caption-attachment-16825" class="wp-caption-text">Forma Steri-Cycle i250 CR CO<sub>2</sub>インキュベーター</p></div> <h2><span id="i">閉鎖系で非接着細胞の拡大培養が可能なバイオリアクターの登場</span></h2> <p><a href="https://www.wilsonwolf.com/" rel="noopener" target="_blank">Wilson Wolf社</a>が設計・開発した<a href="https://www.youtube.com/watch?v=tpuOklz60yQ" rel="noopener" target="_blank">G-Rex™バイオリアクター</a>は、T細胞、CHO細胞、そしてSF9細胞などの非接着性細胞に適した予測可能かつ効率的でスケーラブルな細胞増殖を可能にします。例えば、G-Rex™ 500M-CSバイオリアクターは1台で500 cm<sup>2</sup>のガス透過性膜表面積を有し、5,000 mLのフレッシュな培地を細胞に供給し続けることが可能です。約10日間の培地交換が不要なため、この期間中は閉鎖系で2.5 × 10<sup>8</sup>個の細胞を1.0～2.0 × 10<sup>10</sup>個まで拡大培養できます。さらに、1台のCO<sub>2</sub>インキュベーターがあれば複数のドナー由来の細胞培養に対応でき、前臨床から商用規模の製造まで細胞ベースの治療に必要な多数の細胞を生産することができます。欧米ではCAR-T細胞療法の拡大培養工程におけるゴールドスタンダードとして採用されています。</p> <p><b>G-Rexバイオリアクターの特長</b></p> <ul> <li>既存のCO<sub>2</sub>インキュベーターが使えるため、新規の設備投資が不要</li> <li>細胞に栄養分を無制限に供給でき、培地交換が不要または培地交換の回数を大幅に削減可能</li> <li>底面からガスを安定供給でき、高密度な培養が可能</li> <li>セミオートや閉鎖系での操作も可能</li> <li>T細胞などの免疫細胞、ナチュラルキラー細胞、造血細胞に理想的な生産プラットフォーム</li> </ul> <div id="attachment_16841" style="width: 542px" class="wp-caption aligncenter"><img loading="lazy" decoding="async" aria-describedby="caption-attachment-16841" src="https://www.thermofisher.com/blog/learning-at-the-bench/wp-content/uploads/sites/13/2024/04/d6d5d6e65c264402d1acf30c78d53713-1024x758.jpg" alt="Wilson Wolf社G-Rex 500M-CSバイオリアクター" width="532" height="394" class="size-large wp-image-16841" srcset="https://www.thermofisher.com/blog/learning-at-the-bench/wp-content/uploads/sites/13/2024/04/d6d5d6e65c264402d1acf30c78d53713-1024x758.jpg 1024w, https://www.thermofisher.com/blog/learning-at-the-bench/wp-content/uploads/sites/13/2024/04/d6d5d6e65c264402d1acf30c78d53713-300x222.jpg 300w, https://www.thermofisher.com/blog/learning-at-the-bench/wp-content/uploads/sites/13/2024/04/d6d5d6e65c264402d1acf30c78d53713-768x569.jpg 768w, https://www.thermofisher.com/blog/learning-at-the-bench/wp-content/uploads/sites/13/2024/04/d6d5d6e65c264402d1acf30c78d53713-1536x1137.jpg 1536w, https://www.thermofisher.com/blog/learning-at-the-bench/wp-content/uploads/sites/13/2024/04/d6d5d6e65c264402d1acf30c78d53713-2048x1516.jpg 2048w" sizes="(max-width: 532px) 100vw, 532px" /><p id="caption-attachment-16841" class="wp-caption-text">Wilson Wolf社G-Rex 500M-CSバイオリアクター</p></div> <h2><span id="25">低コストで非接着細胞の生産性を2.5倍に増強する新しいシェルフシステム</span></h2> <p>当社は、これらの細胞加工施設に従事するお客さまが直面する課題に対応するため、Forma Steri-Cycle i250／i250 CR CTSシリーズのCO<sub>2</sub>インキュベーター（以下、Forma Steri-Cycle i250／i250 CR CTS）の庫内空間をG-Rexバイオリアクター用に最適化したThermo Scientific™ CultiMaxx™ シェルフシステムを開発しました。現在お使いいただいているシェルフと入れ替えるだけで、G-Rexバイオリアクターの収容数を最大化し拡大培養の生産性を大幅に増強することができます。</p> <p><b>CultiMaxxシェルフシステムの特長</b></p> <ul> <li>Forma Steri-Cycle i250／i250 CR CTS 1ユニットあたり、G-Rex 500M-CSバイオリアクターの収容数を4個から10個に増強</li> <li>G-Rex 100M-CSバイオリアクターであれば、最大45個、G-Rex 10M-CSバイオリアクターは最大123個収容可能</li> <li>CO<sub>2</sub>インキュベーター1ユニットあたりの耐荷重は最大65 kg</li> <li>強化型シェルフのためたわみにくく、細胞の局在化を防止</li> <li>Forma Steri-Cycle i250／i250 CR CTSは2段積み設置が可能なため、培養室の設置面積あたりのG-Rex 500M-CSバイオリアクターの収容数を2.5倍にアップ（2台分の収容数8個→20個)</li> <li>既存のForma Steri-Cycle i250／i250 CR CTSはそのままで、シェルフだけを入れ替えるため低コスト</li> </ul> <p><img loading="lazy" decoding="async" src="https://www.thermofisher.com/blog/learning-at-the-bench/wp-content/uploads/sites/13/2024/04/3b2139b64fdd6e139f9c6a40942ba8e9-1024x407.png" alt="" width="760" height="302" class="aligncenter size-large wp-image-16837" srcset="https://www.thermofisher.com/blog/learning-at-the-bench/wp-content/uploads/sites/13/2024/04/3b2139b64fdd6e139f9c6a40942ba8e9-1024x407.png 1024w, https://www.thermofisher.com/blog/learning-at-the-bench/wp-content/uploads/sites/13/2024/04/3b2139b64fdd6e139f9c6a40942ba8e9-300x119.png 300w, https://www.thermofisher.com/blog/learning-at-the-bench/wp-content/uploads/sites/13/2024/04/3b2139b64fdd6e139f9c6a40942ba8e9-768x306.png 768w, https://www.thermofisher.com/blog/learning-at-the-bench/wp-content/uploads/sites/13/2024/04/3b2139b64fdd6e139f9c6a40942ba8e9-1536x611.png 1536w, https://www.thermofisher.com/blog/learning-at-the-bench/wp-content/uploads/sites/13/2024/04/3b2139b64fdd6e139f9c6a40942ba8e9-2048x815.png 2048w" sizes="(max-width: 760px) 100vw, 760px" /><br /> Forma Steri-Cycle i250／i250 CR CTSの標準シェルフ（左)では、G-Rex 500M-CSバイオリアクターを最大4個(2個×2段)しか収納できなかったのに対し、CultiMaxxシェルフシステムでは (右)、最大10個(4個×2段+2個×1段)収容できます。</p> <p><img loading="lazy" decoding="async" src="https://www.thermofisher.com/blog/learning-at-the-bench/wp-content/uploads/sites/13/2024/04/a60cf42c84437ed25db605f2bc4d9130-1024x544.jpg" alt="" width="760" height="404" class="aligncenter size-large wp-image-16855" srcset="https://www.thermofisher.com/blog/learning-at-the-bench/wp-content/uploads/sites/13/2024/04/a60cf42c84437ed25db605f2bc4d9130-1024x544.jpg 1024w, https://www.thermofisher.com/blog/learning-at-the-bench/wp-content/uploads/sites/13/2024/04/a60cf42c84437ed25db605f2bc4d9130-300x159.jpg 300w, https://www.thermofisher.com/blog/learning-at-the-bench/wp-content/uploads/sites/13/2024/04/a60cf42c84437ed25db605f2bc4d9130-768x408.jpg 768w, https://www.thermofisher.com/blog/learning-at-the-bench/wp-content/uploads/sites/13/2024/04/a60cf42c84437ed25db605f2bc4d9130-1536x816.jpg 1536w, https://www.thermofisher.com/blog/learning-at-the-bench/wp-content/uploads/sites/13/2024/04/a60cf42c84437ed25db605f2bc4d9130-2048x1089.jpg 2048w" sizes="(max-width: 760px) 100vw, 760px" /><br /> <img loading="lazy" decoding="async" src="https://www.thermofisher.com/blog/learning-at-the-bench/wp-content/uploads/sites/13/2024/04/8a16ab0976c589f1b0333b322aa37ed7-1024x559.jpg" alt="" width="760" height="415" class="aligncenter size-large wp-image-16853" srcset="https://www.thermofisher.com/blog/learning-at-the-bench/wp-content/uploads/sites/13/2024/04/8a16ab0976c589f1b0333b322aa37ed7-1024x559.jpg 1024w, https://www.thermofisher.com/blog/learning-at-the-bench/wp-content/uploads/sites/13/2024/04/8a16ab0976c589f1b0333b322aa37ed7-300x164.jpg 300w, https://www.thermofisher.com/blog/learning-at-the-bench/wp-content/uploads/sites/13/2024/04/8a16ab0976c589f1b0333b322aa37ed7-768x419.jpg 768w, https://www.thermofisher.com/blog/learning-at-the-bench/wp-content/uploads/sites/13/2024/04/8a16ab0976c589f1b0333b322aa37ed7-1536x838.jpg 1536w, https://www.thermofisher.com/blog/learning-at-the-bench/wp-content/uploads/sites/13/2024/04/8a16ab0976c589f1b0333b322aa37ed7-2048x1118.jpg 2048w" sizes="(max-width: 760px) 100vw, 760px" /><br /> G-RexバイオリアクターはG-Rex 500M-CSバイオリアクター(500 cm<sup>2</sup>)だけでなく、ガス透過性膜表面積の異なるG-Rex™ 100M-CS (100 cm<sup>2</sup>)およびG-Rex™ 10M-CS (10 cm<sup>2</sup>)バイオリアクターが用意されています。CultiMaxxシェルフシステムはこれらのバイオリアクターのハイスループット培養にも適しています。<br /> ※（　）内の値はガス透過性膜表面積</p> <h2><span id="i-2">まとめ</span></h2> <p>当社Forma Steri-Cycle i250／i250 CR CTSのCO<sub>2</sub>インキュベーターに革新的なCultiMaxxシェルフシステムを導入することで、Wilson Wolf社のG-Rexバイオリアクターを使用した浮遊細胞の生産性を大幅に増強できます。既存のForma Steri-Cycle i250／i250 CR CTSをそのまま利用できるため、低コストでの導入が可能です。</p> <h3><span id="G-RexCultiMaxx">G-Rex™バイオリアクター向けのCultiMaxxシステム</span></h3> <p><a class="btn btn-red" href="https://www.thermofisher.com/jp/ja/home/life-science/lab-equipment/co2-incubators/accessories.html?CID=lpd_led_cls_r04_jp_cp0000_pjt0000_lpd00000_0so_blg_cell_culture_laboratory_lpd_24018_Social_LAB" target="_blank" rel="noopener noreferrer">詳細はこちら</a> </p> <p>G-Rex is a trademark of Wilson Wolf Corporation.<br /> OPC UA is a registered trademark of OPC Foundation.<br />  </p> <p>研究用にのみ使用できます。診断用には使用いただけません。</p> <aside class="mashsb-container mashsb-main "><div class="mashsb_above_buttons">Share this article </div><div class="mashsb-box"><div class="mashsb-buttons"><a class="mashicon-facebook mash-large mashsb-noshadow" href="https://www.facebook.com/sharer.php?u=https%3A%2F%2Fwww.thermofisher.com%2Fblog%2Flearning-at-the-bench%2Fcell-culture-laboratory-lpd-24018%2F" target="_top" rel="nofollow"><span class="icon"></span><span class="text">Facebook</span></a><a class="mashicon-twitter mash-large mashsb-noshadow" 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