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href="/services/target-specific-assays/protease-assays"><span class="menu-item-link-wrapper">Protease Assays</span></a></li> <li id="menu-item-8705" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-8705"><a title="Kinesin ATPase Assays" href="https://www.reactionbiology.com/services/target-specific-assays/kinesin"><span class="menu-item-link-wrapper">Kinesin ATPase Assays</span></a></li> <li id="menu-item-407" class="menu-item menu-item-type-custom menu-item-object-custom menu-item-407"><a title="Apoptosis Pathway Assays" href="/services/target-specific-assays/apoptosis-pathway-assays"><span class="menu-item-link-wrapper">Apoptosis Pathway Assays</span></a></li> <li id="menu-item-408" class="view-inner-dropdown dropdown-label-2 menu-item menu-item-type-custom menu-item-object-custom menu-item-has-children menu-item-408 dropdown sub-menu"><a title="Metabolic Pathway Assays" href="#"><span class="menu-item-link-wrapper">Metabolic Pathway Assays</span></a> <span 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AMP and cyclic GMP. Inhibition of PDEs prevents the regulation of these second messengers and was found useful in the treatment of a variety of conditions including pulmonary hypertension, acute refractory cardiac failure, erectile dysfunction, etc.</p> <ul> <li>The activity of PDEs is measured with the Transcreener AMP2/GMP2 FP PDE assay platform (BellBrook labs)</li> <li>Low and large scale screening, as well as high-throughput PDE screening options, are available</li> <li>Custom-tailored PDE assay development possible</li> </ul> <p>At Reaction Biology, we offer PDE screening services for pharma, biotech, and academic institutions with a commitment to quality data and quality service. 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paragraph--view-mode--default"><div id="pas-id" class="paragraph excel-export paragraph--type--table-one paragraph--view-mode--default"><div class="container center"><h2>List of PDE Assays (16 total)</h2> <div class="field field--name-field-table-one field--type-tablefield field--label-hidden field__item"> <div id="tablefield-wrapper-paragraph-11429-field_table_one-0" class="tablefield-wrapper"> <div id="tablefield-paragraph-11429-field_table_one-0_wrapper" class="dataTables_wrapper no-footer"> <table id="tablefield-paragraph-11429-field_table_one-0" class="tablefield dataTable no-footer dtr-inline" role="grid" data-striping="1"> <thead> <tr role="row"> <th class="row_0 col_0 all control sorting_asc" tabindex="0" colspan="1" rowspan="1" aria-controls="tablefield-paragraph-11429-field_table_one-0" aria-sort="ascending" aria-label="Target: activate to sort column descending">Target</th> <th class="row_0 col_1 min-fablet sorting" tabindex="0" colspan="1" rowspan="1" aria-controls="tablefield-paragraph-11429-field_table_one-0" aria-label="Synonyms: activate to sort column ascending">Synonyms</th> <th class="row_0 col_2 min-fablet sorting" tabindex="0" colspan="1" rowspan="1" aria-controls="tablefield-paragraph-11429-field_table_one-0" aria-label="UniProt: activate to sort column ascending">UniProt</th> <th class="row_0 col_3 min-tablet sorting" tabindex="0" colspan="1" rowspan="1" aria-controls="tablefield-paragraph-11429-field_table_one-0" aria-label="Data: activate to sort column ascending">Data</th> </tr> </thead> <tbody> <tr class="odd" role="row"> <td class="row_1 col_0 all control sorting_1" tabindex="0">PDE10A</td> <td class="row_1 col_1 min-fablet">cAMP and cAMP-inhibited cGMP 3′,5′-cyclic phosphodiesterase 10A</td> <td class="row_1 col_2 link_text min-fablet"><a class="link_text" href="http://www.uniprot.org/uniprot/Q9Y233">Q9Y233</a></td> <td class="row_1 col_3 datasheet min-tablet"><a class="datasheet overlay" href="https://www.reactionbiology.com/datasheet/pde10a_pde_malvern" target="_blank" rel="noopener">Data sheet</a></td> </tr> <tr class="even" role="row"> <td class="row_2 col_0 all control sorting_1" tabindex="0">PDE1A</td> <td class="row_2 col_1 min-fablet">Calcium/calmodulin-dependent 3′,5′-cyclic nucleotide phosphodiesterase 1A</td> <td class="row_2 col_2 link_text min-fablet"><a class="link_text" href="http://www.uniprot.org/uniprot/P54750">P54750</a></td> <td class="row_2 col_3 datasheet min-tablet"><a class="datasheet overlay" href="https://www.reactionbiology.com/datasheet/pde1a_pde_malvern" target="_blank" rel="noopener">Data sheet</a></td> </tr> <tr class="odd" role="row"> <td class="row_3 col_0 all control sorting_1" tabindex="0">PDE1B</td> <td class="row_3 col_1 min-fablet">Calcium/calmodulin-dependent 3′,5′-cyclic nucleotide phosphodiesterase 1B</td> <td class="row_3 col_2 link_text min-fablet"><a class="link_text" href="http://www.uniprot.org/uniprot/Q01064">Q01064</a></td> <td class="row_3 col_3 datasheet min-tablet"><a class="datasheet overlay" href="https://www.reactionbiology.com/datasheet/pde1b_pde_malvern" target="_blank" rel="noopener">Data sheet</a></td> </tr> <tr class="even" role="row"> <td class="row_4 col_0 all control sorting_1" tabindex="0">PDE1C</td> <td class="row_4 col_1 min-fablet">Calcium/calmodulin-dependent 3′,5′-cyclic nucleotide phosphodiesterase 1C</td> <td class="row_4 col_2 link_text min-fablet"><a class="link_text" href="http://www.uniprot.org/uniprot/Q14123">Q14123</a></td> <td class="row_4 col_3 datasheet min-tablet"><a class="datasheet overlay" href="https://www.reactionbiology.com/datasheet/pde1c_pde_malvern" target="_blank" rel="noopener">Data sheet</a></td> </tr> <tr class="odd" role="row"> <td class="row_5 col_0 all control sorting_1" tabindex="0">PDE2A</td> <td class="row_5 col_1 min-fablet">cGMP-dependent 3′,5′-cyclic phosphodiesterase</td> <td class="row_5 col_2 link_text min-fablet"><a class="link_text" href="http://www.uniprot.org/uniprot/O00408">O00408</a></td> <td class="row_5 col_3 datasheet min-tablet"><a class="datasheet overlay" href="https://www.reactionbiology.com/datasheet/pde2a_pde_malvern" target="_blank" rel="noopener">Data sheet</a></td> </tr> <tr class="even" role="row"> <td class="row_6 col_0 all control sorting_1" tabindex="0">PDE3A</td> <td class="row_6 col_1 min-fablet">cGMP-inhibited 3′,5′-cyclic phosphodiesterase A</td> <td class="row_6 col_2 link_text min-fablet"><a class="link_text" href="http://www.uniprot.org/uniprot/Q14432">Q14432</a></td> <td class="row_6 col_3 datasheet min-tablet"><a class="datasheet overlay" href="https://www.reactionbiology.com/datasheet/pde3a_pde_malvern" target="_blank" rel="noopener">Data sheet</a></td> </tr> <tr class="odd" role="row"> <td class="row_7 col_0 all control sorting_1" tabindex="0">PDE3B</td> <td class="row_7 col_1 min-fablet">cGMP-inhibited 3′,5′-cyclic phosphodiesterase B</td> <td class="row_7 col_2 link_text min-fablet"><a class="link_text" href="http://www.uniprot.org/uniprot/Q13370">Q13370</a></td> <td class="row_7 col_3 datasheet min-tablet"><a class="datasheet overlay" href="https://www.reactionbiology.com/datasheet/pde3b_pde_malvern" target="_blank" rel="noopener">Data sheet</a></td> </tr> <tr class="even" role="row"> <td class="row_8 col_0 all control sorting_1" tabindex="0">PDE4A</td> <td class="row_8 col_1 min-fablet">cAMP-specific 3′,5′-cyclic phosphodiesterase 4A</td> <td class="row_8 col_2 link_text min-fablet"><a class="link_text" href="http://www.uniprot.org/uniprot/P27815">P27815</a></td> <td class="row_8 col_3 datasheet min-tablet"><a class="datasheet overlay" href="https://www.reactionbiology.com/datasheet/pde4a_pde_malvern" target="_blank" rel="noopener">Data sheet</a></td> </tr> <tr class="odd" role="row"> <td class="row_9 col_0 all control sorting_1" tabindex="0">PDE4B</td> <td class="row_9 col_1 min-fablet">cAMP-specific 3′,5′-cyclic phosphodiesterase 4B</td> <td class="row_9 col_2 link_text min-fablet"><a class="link_text" href="http://www.uniprot.org/uniprot/Q07343">Q07343</a></td> <td class="row_9 col_3 datasheet min-tablet"><a class="datasheet overlay" href="https://www.reactionbiology.com/datasheet/pde4b_pde_malvern" target="_blank" rel="noopener">Data sheet</a></td> </tr> <tr class="even" role="row"> <td class="row_10 col_0 all control sorting_1" tabindex="0">PDE4C</td> <td class="row_10 col_1 min-fablet">cAMP-specific 3′,5′-cyclic phosphodiesterase 4C</td> <td class="row_10 col_2 link_text min-fablet"><a class="link_text" href="http://www.uniprot.org/uniprot/Q08493">Q08493</a></td> <td class="row_10 col_3 datasheet min-tablet"><a class="datasheet overlay" href="https://www.reactionbiology.com/datasheet/pde4c_pde_malvern" target="_blank" rel="noopener">Data sheet</a></td> </tr> <tr class="odd" role="row"> <td class="row_11 col_0 all control sorting_1" tabindex="0">PDE4D</td> <td class="row_11 col_1 min-fablet">cAMP-specific 3′,5′-cyclic phosphodiesterase 4D</td> <td class="row_11 col_2 link_text min-fablet"><a class="link_text" href="http://www.uniprot.org/uniprot/Q08499">Q08499</a></td> <td class="row_11 col_3 datasheet min-tablet"><a class="datasheet overlay" href="https://www.reactionbiology.com/datasheet/pde4d_pde_malvern" target="_blank" rel="noopener">Data sheet</a></td> </tr> <tr class="even" role="row"> <td class="row_12 col_0 all control sorting_1" tabindex="0">PDE5A</td> <td class="row_12 col_1 min-fablet">cGMP-specific 3′,5′-cyclic phosphodiesterase</td> <td class="row_12 col_2 link_text min-fablet"><a class="link_text" href="http://www.uniprot.org/uniprot/O76074">O76074</a></td> <td class="row_12 col_3 datasheet min-tablet"><a class="datasheet overlay" href="https://www.reactionbiology.com/datasheet/pde5a_pde_malvern" target="_blank" rel="noopener">Data sheet</a></td> </tr> <tr class="odd" role="row"> <td class="row_13 col_0 all control sorting_1" tabindex="0">PDE7A</td> <td class="row_13 col_1 min-fablet">High affinity cAMP-specific 3′,5′-cyclic phosphodiesterase 7A</td> <td class="row_13 col_2 link_text min-fablet"><a class="link_text" href="http://www.uniprot.org/uniprot/Q13946">Q13946</a></td> <td class="row_13 col_3 datasheet min-tablet"><a class="datasheet overlay" href="https://www.reactionbiology.com/datasheet/pde7a_pde_malvern" target="_blank" rel="noopener">Data sheet</a></td> </tr> <tr class="even" role="row"> <td class="row_16 col_0 all control sorting_1" tabindex="0">PDE7B</td> <td class="row_16 col_1 min-fablet">cAMP-specific 3′,5′-cyclic phosphodiesterase 7B</td> <td class="row_16 col_2 link_text min-fablet"><a class="link_text" href="http://www.uniprot.org/uniprot/Q9NP56">Q9NP56</a></td> <td class="row_16 col_3 datasheet min-tablet"><a class="datasheet overlay" href="https://www.reactionbiology.com/datasheet/pde7b_pde_malvern" target="_blank" rel="noopener">Data sheet</a></td> </tr> <tr class="odd" role="row"> <td class="row_14 col_0 all control sorting_1" tabindex="0">PDE8A</td> <td class="row_14 col_1 min-fablet">High affinity cAMP-specific and IBMX-insensitive 3′,5′-cyclic phosphodiesterase 8A</td> <td class="row_14 col_2 link_text min-fablet"><a class="link_text" href="http://www.uniprot.org/uniprot/O60658">O60658</a></td> <td class="row_14 col_3 datasheet min-tablet"><a class="datasheet overlay" href="https://www.reactionbiology.com/datasheet/pde8a_pde_malvern" target="_blank" rel="noopener">Data sheet</a></td> </tr> <tr class="even" role="row"> <td class="row_15 col_0 all control sorting_1" tabindex="0">PDE9A</td> <td class="row_15 col_1 min-fablet">High affinity cGMP-specific 3′,5′-cyclic phosphodiesterase 9A</td> <td class="row_15 col_2 link_text min-fablet"><a class="link_text" href="http://www.uniprot.org/uniprot/O76083">O76083</a></td> <td class="row_15 col_3 datasheet min-tablet"><a class="datasheet overlay" href="https://www.reactionbiology.com/datasheet/pde9a_pde_malvern" target="_blank" rel="noopener">Data sheet</a></td> </tr> </tbody> </table> </div> </div> </div> </div></div><div id="pas-id2" class="paragraph paragraph--type--tab-container paragraph--view-mode--default"><div class="container center"><h2>PDE Screening Details</h2> <div class="tab-container"><ul class="tab-navigation"><li class="tab-active" data-id="#tab-pas-id221">Assay Format </li><li data-id="#tab-pas-id222">Assay Setup </li><li data-id="#tab-pas-id223">Monthly Screening Schedule</li></ul><div class="field field--name-field-paragraph field--type-entity-reference-revisions field--label-hidden field__items"><div id="tab-pas-id221" class="tab-content-active paragraph paragraph--type--tab-text-with-image paragraph--view-mode--default"><div class="field field--name-field-title field--type-string field--label-hidden field__items">Assay Format </div><div class="tab-content"><div class="tab-content-wrapper"><div class="clearfix text-formatted field field--name-field-text field--type-text-long field--label-hidden field__items"><p><img loading="lazy" decoding="async" class="alignnone size-full wp-image-956" src="https://www.reactionbiology.com/wp-content/uploads/2023/06/PDE_assay-principle.jpg" alt="" width="760" height="173" srcset="https://www.reactionbiology.com/wp-content/uploads/2023/06/PDE_assay-principle.jpg 760w, https://www.reactionbiology.com/wp-content/uploads/2023/06/PDE_assay-principle-300x68.jpg 300w" sizes="(max-width: 760px) 100vw, 760px" /><br /> PDE converts cAMP or cGMP to AMP or GMP which displace a fluorescent tracer from an antibody selective for AMP and GMP resulting in a reduction of the fluorescence polarization signal.</p> </div></div></div></div><div id="tab-pas-id222" class=" paragraph paragraph--type--tab-text-with-image paragraph--view-mode--default"><div class="field field--name-field-title field--type-string field--label-hidden field__items">Assay Setup </div><div class="tab-content"><div class="tab-content-wrapper"><div class="clearfix text-formatted field field--name-field-text field--type-text-long field--label-hidden field__items"><div class="tab-content"> <div class="tab-content-wrapper"> <div class="clearfix text-formatted field field--name-field-text field--type-text-long field--label-hidden field__items"> <div class="field__item"> <p><strong>Setups</strong>: Single-dose screening in duplicates or IC50 value determination with 5 or 10 concentrations. Other screening formats are available upon request.</p> <p><strong>Controls</strong>: No inhibitor (DMSO vehicle) control and for every assay, one target-specific control compound is tested in 10-dose IC50 format.</p> <p><strong>Turnaround time</strong>: 10 business days for standard projects. Expedited scheduling and data delivery can be arranged prior to the commencement of the studies.</p> <p><strong>Report</strong>: The raw data, % enzyme activity and control compound IC50 values will be reported in Excel format for single-dose assays. For IC50 orders, raw data, IC50 values, and curve fitting will be delivered in Excel format. Assay conditions, target, and substrate information are available upon request. Requirements for this information should be noted prior to the commencement of the study.</p> <p><strong>Screening facility</strong>: This assay is performed at our screening facility in Malvern, PA, US.</p> <p><strong>Compound requirements</strong>: In brief, for a standard project, 20 µl of a 10 mM DMSO stock or solid material is needed. Less material is needed for large scale screening. Please refer to our <a href="https://www.reactionbiology.com/FAQ">FAQs</a> for information regarding compound preparation and shipping.</p> </div> </div> <div class="field field--name-field-pdf field--type-file field--label-hidden field__items"> <div class="field__item"><span class="file file--mime-application-vnd-openxmlformats-officedocument-spreadsheetml-sheet file--x-office-spreadsheet"> <a title="FAQ Assay Condition Form" href="https://www.reactionbiology.com/faq/" target="_blank" rel="noopener">Please see our FAQ page to access our assay condition form (US facility).</a></span></div> </div> </div> </div> </div></div></div></div><div id="tab-pas-id223" class=" paragraph paragraph--type--tab-text-with-image paragraph--view-mode--default"><div class="field field--name-field-title field--type-string field--label-hidden field__items">Monthly Screening Schedule</div><div class="tab-content"><div class="tab-content-wrapper"><div class="clearfix text-formatted field field--name-field-text field--type-text-long field--label-hidden field__items"><div class="tab-content"> <div class="tab-content-wrapper"> <div class="clearfix text-formatted field field--name-field-text field--type-text-long field--label-hidden field__items"> <div class="field__item"> <p>On a monthly basis, Reaction Biology performs compound screening on the full phosphodiesterase panel.</p> <p>Please see the upcoming screening dates <a href="https://www.reactionbiology.com/phosphodiesterase-full-panel-schedule">here</a>.</p> </div> </div> </div> </div> </div></div></div></div></div></div></div></div><div id="pas-id4" class="paragraph paragraph--type--related-services-reference-cont paragraph--view-mode--default"><div class="container center"><div class="field field--name-field-related-service-container field--type-entity-reference-revisions field--label-above field__items"><h2>Related Services</h2><div class="field--name-field-paragraph slick-slider"><div class="paragraph 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