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vertical-align: middle;"><h1 style="color: rgb(255, 255, 255);">Live-Cell Imaging</h1><h5 style="color: rgb(255, 255, 255);">Learn the simple steps to stain your live cells for dynamic imaging experiments.</h5></td></tr></tbody></table></div></div></div></div></div></div></div></div></div></div></div><div class="container page-header-hidden"><div class="row"><div class="span12"><div class="pageheading"></div></div></div></div><div class="container-wrap template-2-column"><div class="container"><div class="row"><script> var showHideText = { 'seeRelatedText':'See Navigation', 'hideRelatedText':'Hide Navigation' }; </script><div class="span3 sidebar" role="complementary"><div id="link-leftnav-toggle-container"><a class='link-leftnav-toggle' href='#'>See Navigation</a></div><div class="container-leftnav"><div class="railInheritParsys iparsys parsys"><div class="section"><div class="new"></div></div><div class="iparys_inherited"><div class="railInheritParsys iparsys parsys"><div class="railsection section"><div class="leftnav-standard"><div class="nav-list-header"><a href="/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if.html"><span class="nav-list-header-icon">&lsaquo;</span><span class="nav-list-header-text">Fluorescence Microscopy Reagents</span></a></div><ul class="nav nav-list" id="navList"><li class="item-no-children"><a class="" href="/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/fixed-cell-imaging-reagents.html">5 Steps to Fixed-Cell Imaging <span class="nav-list-arrow">&rsaquo;</span></a></li><li class="active" id="navListActive"><a class="item-header" href="/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents.html">Live-Cell Imaging <span class="nav-list-arrow">&rsaquo;</span></a></li><li class="item-no-children"><a class="" href="/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/microscope-calibration-reagents.html">Microscope Calibration Reagents <span class="nav-list-arrow">&rsaquo;</span></a></li></ul><script> if ( $('#navListActive').children('ul').length > 0 ) { var activeNav = $j('#navListActive'); 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$j('#navList').prepend(activeNav); } </script></div></div><div class="leftnav section"><div class="leftnav-manual"><ul class="nav nav-list"><li class="item-header nav-header"> Learning & Support <span class="item-icon icon-plus pull-right visible-sm visible-xs"></span></li><li class="item-content collapse" ><ul><li><a href="/sg/en/home/life-science/cell-analysis/cell-analysis-learning-center.html">Cell Analysis Learning Center</a></li><li><a href="/sg/en/home/technical-resources/technical-reference-library/cell-analysis-support-center.html">Cell Analysis Support Center</a></li><li><a href="/sg/en/home/life-science/cell-analysis/cellular-imaging/guide-stain-it.html">Stain-iT Cell Staining Simulator Guide</a></li></ul></li></ul></div></div></div></div></div><div class="railParsys parsys"></div></div></div><div class="span9 main" role="main"><div class="MainParsys parsys"><div class="heading section"><h2> What is fluorescence live-cell imaging? </h2></div><div class="text parbase section"><p>Fluorescence imaging of live cells is used to observe dynamic cellular processes and track cellular biomolecules and structures over time. Live-cell fluorescence imaging allows researchers to examine cells in their native state so that cellular function is preserved, enabling the study of cellular processes including <a href="/sg/en/home/life-science/cell-analysis/cell-structure/cytoskeleton.html">cytoskeletal</a> rearrangement, <a href="/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/imaging-apoptosis-assays.html">apoptosis</a>, <a href="/sg/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology/cell-tracking.html">cell migration</a>, <a href="/sg/en/home/life-science/cell-analysis/cell-viability-and-regulation/endocytosis-exocytosis-and-phagocytosis/endocytosis-and-pinocytosis.html">endocytosis</a>, <a href="/sg/en/home/life-science/cell-analysis/cell-viability-and-regulation/endocytosis-exocytosis-and-phagocytosis/phagocytosis.html">phagocytosis</a>, and organelle dynamics to obtain more relevant findings in drug discovery, cancer, developmental biology, environmental science, and other medical research fields.</p><p>Use this guide to build live-cell imaging experiments, pick reagents, and generate publication quality data.</p></div><div class="well-cmp section"><div class="well "><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><h3> Build live-cell experiments for your imaging platform with an incubator or microscope </h3></div><div class="brightcoveplayer section"><div data-video="82c67f831c34"><script type="text/javascript"> var loadEME = true; 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margin-left: auto; margin-right: auto; margin-top: 0; overflow-x: hidden; overflow-y: hidden; text-align: center; width: 100%; text-align: center;"><div id="container-82c67f831c34" class="brightcove-container"><script>document.createElement('video')</script><video id="video-82c67f831c34" data-account="3663210762001" data-player="08UsfMRkC" data-embed="default" data-video-id="6335042573112" class="video-js" controls ></video><script src="//players.brightcove.net/3663210762001/08UsfMRkC_default/index.min.js"></script><script> videojs.options.techOrder = ["html5"]; </script></div></div></div></div><script src="//players.brightcove.net/3663210762001/08UsfMRkC_default/index.min.js"></script><script type="text/javascript"> var schemaObj = { "@context": "https://schema.org", "@type": "VideoObject", "name": "CellLight Imaging movie", "description": "Cell transfected with CellLight™ Tubulin-GFP in green and CellLight™ Actin-RFP in red", "thumbnailUrl": ["https://cf-images.us-east-1.prod.boltdns.net/v1/static/3663210762001/04c4f012-49e8-4dae-b4a3-623062ef62eb/c938d605-4088-4823-8207-533818d75883/160x90/match/image.jpg"], "uploadDate": "2023-08-21T09:57:06.788Z", "duration": "PT14.467S", "contentUrl": "https://edge.api.brightcove.com/playback/v1/accounts/3663210762001/videos/6335042573112/high.mp4?bcov_auth=eyJhbGciOiAiUlMyNTYiLCJ0eXAiOiAiSldUIn0.ewogICJpc3MiOiAiaHR0cHM6Ly93d3cudGhlcm1vZmlzaGVyLmNvbS8iLAogICJzdWIiOiAiY29udGVudF91cmwiLAogICJhdWQiOiAiaHR0cHM6Ly9icmlnaHRjb3ZlLmNvbSIKfQ.YqHusDPupPtMxztEAJqXZdheYqO3snN6xjms4Ui3zIPoj2yxzflQiCo4F8WHxA9NYzu3QGhPfCS_m8RFrLoa656z7RNfPGWsxJRa-IPjFvLAqhwJiJNWKBMoClWyGj0-groti1dghXWuGXxNraTU5GZ92tReZd9H8-g1ugo2hP_CV_qPtN8_uk6049UR1uXSXkiVf-bHrShqG1GDrTELzsHkLy3QAbyFkSlITfeaKcqGQO0nmAMPxOPbqHF5WjQwJEZyRmHOOGLXcy1PMRSUvtT9LUpJ6NOz8wgw6dfLpqdxnZOuSxwxTC6WRcnRSiokgpYuP6zYCopaoWUkeIzX3Q", "embedUrl": "//players.brightcove.net/3663210762001/08UsfMRkC_default/index.html?videoId=6335042573112", "@id": "//players.brightcove.net/3663210762001/08UsfMRkC_default/index.html?videoId=6335042573112&_="+new Date().getTime() }; var el = document.createElement("script"); el.type = "application/ld+json"; el.text = JSON.stringify(schemaObj); document.head.appendChild(el); if (navigator.userAgent.indexOf('MSIE') !== -1 || navigator.userAgent.indexOf('Trident/') !== -1) { $('.brightcoveplayer, .cmp-p-videoplayer').addClass('brightcoveplayer-ie'); $("<div class='row' />") .append("<div class='span1 warning-triangle-icon' />") .append("<div class='span11'><b>Error:</b> Internet Explorer is not compatible with the Brightcove Video Player. Please use a different browser to view these videos.</div>") .addClass("unsuppported-videoplayer") .insertAfter($(".brightcoveplayer, .cmp-p-videoplayer")); } </script></div><div class="text parbase section"><p><a href="/order/stain-it/#!/"><span class="btn btn-featured">Use Stain-iT Simulator</span></a></p></div></div></div></div></div></div><div class="well-cmp section"><div class="well "><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><div class="h2"> On this page: </div></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="text parbase section"><ul><li><a href="#live-cell-imaging">How to choose reagents for live-cell imaging</a></li><li><a href="#environmental-control">How to maintain environmental control during live-cell imaging</a></li><li><a href="#apoptosis">Example: apoptosis in live-cell imaging experiments</a></li><li><a href="#mitochondrial">Example: mitochondrial activity in live-cell imaging experiments</a></li></ul></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="text parbase section"><ul><li><a href="#detect-changes">Tips to detect changes in live-cell experiments</a></li><li><a href="#free-webinar">Featured webinar</a></li><li><a href="#images-and-videos">Examples of live-cell images and videos</a></li><li><a href="#imaging-resources">Live-cell imaging resources</a></li></ul></div></div></div></div><div style="clear:both"></div></div></div></div></div></div><div class="text parbase section"><div style="padding: 20px; background: url(/content/dam/LifeTech/global/life-sciences/bid/pca-ais/2025/1/feature-box-900x264.jpg) no-repeat; background-size: cover;"><h2 style="color: #fff;">Enhance your imaging experiments with optimized product pairings</h2><p style="color: #fff;">Save time with carefully matched product pairings designed to deliver exceptional imaging results.</p><p style="margin-bottom: 0;"><a href="/sg/en/home/c/b/enhanced-imaging-optimized-solutions.html" class="btn btn-primary">Explore now</a></p></div></div><div class="heading section"><h2 id="live-cell-imaging"><br>How to choose reagents for live-cell imaging </h2></div><div class="text parbase section"><p>Live-cell imaging reagents include both <a href="/sg/en/home/life-science/cell-analysis/cell-structure/celllight-ready-to-use-fluorescent-protein-based-reagents.html"><u>targeted fluorescent proteins</u></a> and small membrane permeant fluorescent dyes. To enable live-cell imaging experiments, many reagents are designed for time-lapse over several hours or days, while others are optimal for end-point assays in which cells are imaged and analyzed immediately after staining. Staining concentration, incubation time, and the appropriate time window and imaging interval/frequency should be determined empirically to minimize cytotoxicity and preserve cellular function.</p><p>The reagents found within the live-cell imaging guide below are compatible with automated high-content and incubator-based fluorescence imaging systems such as: Thermo Fisher Scientific’s  <a href="/sg/en/home/life-science/cell-analysis/cellular-imaging/evos-cell-imaging-systems.html">EVOS M7000 and M5000 Cell Imaging Systems</a> with <a href="/sg/en/home/life-science/cell-analysis/cellular-imaging/evos-cell-imaging-systems/accessories/onstage-incubator.html">EVOS Onstage Incubator</a>, <a href="/sg/en/home/life-science/cell-analysis/cellular-imaging/hcs-hca.html">CellInsight HCS Platforms</a>, PerkinElmer’s MuviCyte™ live-cell imaging system*, Leica’s Thunder and Mica*, and Sartorius’ Incucyte® Live-Cell Analysis System™*.</p></div><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" id="table--1171493969"><tbody><tr><td rowspan="2"> </td><td style="background-color: #00b0f0; color: #fff;" rowspan="2"><b>EVOS DAPI Light Cube</b></td><td style="background-color: #92d050; color: #fff;"><b>EVOS GFP Light Cube<br> Excitation: 470/22 nm;<br> Emission: 510/42 nm </b></td><td style="background-color: #ffc000; color: #fff;"><b>EVOS RFP Light Cube Excitation: 531/40 nm; Emission: 593/40 nm </b></td><td style="background-color: #ff0000; color: #fff;"><b>EVOS Red Light Cube Excitation: 585/29 nm; Emission: 624/40 nm </b></td><td style="background-color: #7030a0; color: #fff;"><b>EVOS Cy5 Light Cube Excitation: 628/40 nm; Emission: 693/40 nm </b></td><td rowspan="2"><b>Live-cell imaging time frame</b></td></tr><tr><td style="background-color: #92d050; color: #fff;"><b>Incucyte Green Filter</b></td><td style="background-color: #ffc000; color: #fff;"><b>Incucyte Orange Filter</b></td><td style="background-color: #ff0000; color: #fff;"><b>Incucyte Red Filter</b></td><td style="background-color: #7030a0; color: #fff;"><b>Incucyte NIR Filter</b></td></tr><tr><td rowspan="2"><a href="/sg/en/home/life-science/cell-analysis/cell-viability-and-regulation/endocytosis-exocytosis-and-phagocytosis/antibody-internalization.html"><b>Antibody internalization</b></a></td><td rowspan="2"> </td><td rowspan="2"><p><a href="/sg/en/home/life-science/cell-analysis/cell-viability-and-regulation/endocytosis-exocytosis-and-phagocytosis/antibody-internalization.html">pHrodo Green iFL antibody labeling reagents</a></p><p><a href="/sg/en/home/life-science/cell-analysis/cell-viability-and-regulation/endocytosis-exocytosis-and-phagocytosis/antibody-internalization.html">LysoLight Green labeling kits and reagents </a></p></td><td width="79" valign="top" rowspan="2"><p><a href="/sg/en/home/life-science/cell-analysis/cell-viability-and-regulation/endocytosis-exocytosis-and-phagocytosis/antibody-internalization.html">pHrodo Red iFL antibody labeling reagents</a><br></p></td><td rowspan="2"> </td><td><a href="/sg/en/home/life-science/cell-analysis/cell-viability-and-regulation/endocytosis-exocytosis-and-phagocytosis/antibody-internalization.html">pHrodo Deep Red antibody labeling reagents</a></td><td>Long-term (hours to overnight)</td></tr><tr><td width="102" valign="top"><p><a href="/sg/en/home/life-science/cell-analysis/cell-viability-and-regulation/endocytosis-exocytosis-and-phagocytosis/antibody-internalization.html">LysoLight Deep Red labeling kits and reagents</a></p></td><td width="78" valign="top">Long-term (~72 hours)</td></tr><tr><td><a href="/sg/en/home/life-science/antibodies/antibody-labeling.html"><b>Antibody binding</b></a></td><td> </td><td><a href="/order/catalog/product/Z25615">Zenon Alexa Fluor Plus 488</a></td><td> </td><td><a href="/order/catalog/product/Z25616">Zenon Alexa Fluor Plus 594</a></td><td><a href="/order/catalog/product/Z25617">Zenon Alexa Fluor Plus 647</a></td><td>Short-term</td></tr><tr><td rowspan="2"><a href="/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/imaging-apoptosis-assays/imaging-caspase-activity.html"><b>Apoptosis</b></a></td><td> </td><td><a href="/order/catalog/product/C10430">CellEvent Caspase-3/7 Green Detection Reagent</a></td><td width="79" valign="top"><p> </p></td><td><a href="/order/catalog/product/C10430">CellEvent Caspase-3/7 Red Detection Reagent</a></td><td> </td><td>Long-term (overnight to 48-72 hours)</td></tr><tr><td colspan="5" style="text-align: center;"><a href="/sg/en/home/life-science/cell-analysis/cell-viability-and-regulation/apoptosis/annexin-v-staining.html">Annexin V Conjugates</a></td><td>Long-term</td></tr><tr><td><a href="/sg/en/home/life-science/cell-analysis/cell-viability-and-regulation/autophagy.html"><b>Autophagy</b></a></td><td> </td><td><a href="/sg/en/home/life-science/cell-analysis/cell-viability-and-regulation/autophagy/autophagy-lc3b-p62.html#prd1">Premo Autophagy Sensors (p62 and LC3B) GFP</a></td><td><a href="/sg/en/home/life-science/cell-analysis/cell-viability-and-regulation/autophagy/autophagy-lc3b-p62.html#prd1">Premo Autophagy Sensors (p62 and LC3B) RFP</a></td><td> </td><td> </td><td>Long-term (overnight to 48 hours)</td></tr><tr><td rowspan="2"><a href="/sg/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology/cell-tracking.html"><b>Cell tracking</b></a></td><td colspan="5" style="text-align: center;" valign="middle"><a href="/sg/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology/cell-tracking.html#prd-1">CellTracker dyes</a></td><td>Short- to long-term (72 hours/~3 or more generations)</td></tr><tr><td style="text-align: center;"> </td><td><a href="/order/catalog/product/V12883">Vybrant CFDA SE Cell Tracer kit</a></td><td> </td><td> </td><td> </td><td>Long-term</td></tr><tr><td><a href="/sg/en/home/life-science/cell-analysis/cell-structure/cytoskeleton.html#anchor3"><b>Cytoskeleton (actin)</b></a></td><td> </td><td><a href="/order/catalog/product/A57243">CellMask Green Actin Tracking Stain</a></td><td><a href="/order/catalog/product/A57244">CellMask Orange Actin Tracking Stain</a></td><td> </td><td><a href="/order/catalog/product/A57245">CellMask Deep Red Actin Tracking Stain</a></td><td colspan="2">Short- to long-term (24+ hours)</td></tr><tr><td><a href="/sg/en/home/life-science/cell-analysis/cell-structure/cytoskeleton.html#anchor3"><b>Cytoskeleton (tubulin)</b></a></td><td> </td><td><a href="/order/catalog/product/T34078">Tubulin Tracker Green</a></td><td> </td><td> </td><td><a href="/order/catalog/product/T34077">Tubulin Tracker Deep Red</a></td><td colspan="2">Short-term</td></tr><tr><td><a href="/sg/en/home/life-science/cell-analysis/cell-viability-and-regulation/endocytosis-exocytosis-and-phagocytosis/endocytosis-and-pinocytosis.html"><b>Endosomes/endocytosis</b></a></td><td> </td><td colspan="4" style="text-align: center;" valign="middle"><a href="/sg/en/home/life-science/cell-analysis/cell-viability-and-regulation/endocytosis-exocytosis-and-phagocytosis/endocytosis-and-pinocytosis.html#prd">pHrodo and Alexa Fluor dextrans</a></td><td>Short- to long-term (minutes to hours; overnight or longer for some applications)</td></tr><tr><td rowspan="2"><a href="/sg/en/home/life-science/cell-analysis/cell-viability-and-regulation/endocytosis-exocytosis-and-phagocytosis/ligand-internalization.html"><b>Ligand internalization</b></a></td><td rowspan="2"> </td><td colspan="4" style="text-align: center;"><a href="/sg/en/home/life-science/cell-analysis/cell-viability-and-regulation/endocytosis-exocytosis-and-phagocytosis/ligand-internalization.html#prd">Alexa Fluor, BODIPY, and pHrodo LDL conjugates</a></td><td rowspan="2">Short- to long-term (minutes to overnight or longer)</td></tr><tr><td colspan="4" style="text-align: center;"><a href="/sg/en/home/life-science/cell-analysis/cell-viability-and-regulation/endocytosis-exocytosis-and-phagocytosis/ligand-internalization.html#prd">Alexa Fluor and pHrodo EGF and transferrin conjugates</a></td></tr><tr><td><a href="/sg/en/home/life-science/cell-analysis/cell-structure/endoplasmic-reticulum.html"><b>Endoplasmic reticulum</b></a></td><td> </td><td><a href="/order/catalog/product/E34251">ER-Tracker Green</a></td><td><br><br></td><td><a href="/order/catalog/product/E34250">ER-Tracker Red</a></td><td><br><br></td><td>End-point</td></tr><tr><td><b>Hypoxia</b></td><td> </td><td><a href="/order/catalog/product/I14834">Image-iT Green Hypoxia Reagent</a></td><td> </td><td> </td><td> </td><td>End-point </td></tr><tr><td><a href="/sg/en/home/life-science/cell-analysis/cell-viability-and-regulation/ion-indicators/calcium-indicators.html"><b>Calcium</b></a></td><td> </td><td><a href="/order/catalog/product/F10489">Fluo-4 Calcium Imaging Kit</a></td><td><a href="/order/catalog/product/R10145">Rhod-3 Calcium Imaging Kit</a></td><td> </td><td> </td><td>Short-term (minutes to hours)</td></tr><tr><td><a href="/sg/en/home/life-science/cell-analysis/cell-structure/lysosomes.html"><b>Lysosomes</b></a></td><td colspan="5" style="text-align: center;"><a href="/sg/en/home/life-science/cell-analysis/cell-structure/lysosomes.html#prd1">LysoTracker and LysoSensor dyes</a></td><td>Short-term (minutes to hours)</td></tr><tr><td><b>Membranes (plasma and internal)</b></td><td> </td><td colspan="4" style="text-align: center;"><a href="/order/catalog/product/V22889">Vybrant cell labeling solutions</a></td><td>Long-term</td></tr><tr><td><a href="/sg/en/home/life-science/cell-analysis/cell-structure/mitochondria.html"><b>Mitochondria structure</b></a></td><td> </td><td colspan="4" style="text-align: center;"><a href="/sg/en/home/life-science/cell-analysis/cell-structure/mitochondria.html#prd1">MitoTracker dyes</a></td><td>Long-term</td></tr><tr><td rowspan="2"><a href="/sg/en/home/life-science/cell-analysis/cell-viability-and-regulation/apoptosis/mitochondria-function.html"><b>Mitochondria function</b></a></td><td rowspan="2"> </td><td rowspan="2"> </td><td style="text-align: center;"><a href="/order/catalog/product/I34361">TMRM</a></td><td rowspan="2"> </td><td rowspan="2"> </td><td rowspan="2">Short-term (minutes to hours)</td></tr><tr><td style="text-align: center;"><a href="/order/catalog/product/T669">TMRE</a></td></tr><tr><td rowspan="3"><a href="/sg/en/home/life-science/cell-analysis/cell-structure/nucleus-and-nucleoli.html"><b>Nucleus</b></a></td><td><a href="/order/catalog/product/H10325">HCS NuclearMask Blue Stain</a></td><td><a href="/order/catalog/product/S34854">SYTO 9 Green Fluorescent Nucleic Acid Stain</a></td><td> </td><td><a href="/order/catalog/product/H10326">HCS NuclearMask Red Stain</a></td><td><a href="/order/catalog/product/H10294">HCS NuclearMask Deep Red Stain</a></td><td rowspan="3" valign="middle">End-point</td></tr><tr><td><a href="/order/catalog/product/H3570">Hoechst 33342</a></td><td> </td><td> </td><td><a href="/order/catalog/product/S11341">SYTO 59 Red Fluorescent Nucleic Acid Stain</a></td><td><a href="/order/catalog/product/R37106">NucRed Live 647 ReadyProbes Reagent</a></td></tr><tr><td><a href="/order/catalog/product/R37605">NucBlue Live ReadyProbes Reagent</a></td><td> </td><td> </td><td> </td><td><a href="/order/catalog/product/62252">DRAQ5 Fluorescent Probe Solution</a></td></tr><tr><td><a href="/sg/en/home/life-science/cell-analysis/cell-structure/plasma-membrane.html"><b>Plasma membrane</b></a></td><td> </td><td><a href="/order/catalog/product/C37608">CellMask Green Plasma Membrane Stain</a></td><td><a href="/order/catalog/product/C10045">CellMask Orange Plasma Membrane Stain</a></td><td> </td><td><a href="/order/catalog/product/C10046">CellMask Deep Red Plasma Membrane Stain</a></td><td>Short-term (10-90 minutes, can image longer but will internalize)</td></tr><tr><td rowspan="3"><a href="/sg/en/home/life-science/cell-analysis/cell-viability-and-regulation/oxidative-stress.html"><b>Reactive oxygen species</b></a></td><td> </td><td><a href="/order/catalog/product/C10444">CellROX Green Reagent</a></td><td><a href="/order/catalog/product/C10443">CellROX Orange Reagent</a></td><td> </td><td><a href="/order/catalog/product/C10422">CellROX Deep Red Reagent</a></td><td>Long-term (24 hours)</td></tr><tr><td> </td><td><a href="/order/catalog/product/C6827">H<sub>2</sub>DCFDA dyes</a></td><td> </td><td> </td><td> </td><td>Short-term (~2 hours)</td></tr><tr><td><a href="/order/catalog/product/T10095">ThiolTracker Violet</a></td><td><a href="/order/catalog/product/M36005">MitoSOX Green Superoxide Indicator</a></td><td> </td><td> </td><td> </td><td>End-point</td></tr><tr><td><a href="/sg/en/home/life-science/cell-analysis/cell-viability-and-regulation/endocytosis-exocytosis-and-phagocytosis/phagocytosis.html"><b>Phagocytosis</b></a></td><td> </td><td><a href="/sg/en/home/brands/molecular-probes/key-molecular-probes-products/phrodo-indicators.html#phagocytosis">pHrodo Green BioParticles Conjugates</a></td><td><a href="/sg/en/home/brands/molecular-probes/key-molecular-probes-products/phrodo-indicators.html#phagocytosis">pHrodo Red BioParticles Conjugates</a></td><td> </td><td><a href="/sg/en/home/brands/molecular-probes/key-molecular-probes-products/phrodo-indicators.html#phagocytosis">pHrodo Deep Red BioParticles Conjugates</a></td><td>Short- to long-term (minutes to hours or longer)  </td></tr><tr><td><a href="/sg/en/home/life-science/cell-analysis/cell-viability-and-regulation/cell-viability.html"><b>Viability (live cell indicators)</b></a></td><td><a href="/order/catalog/product/C34853">Calcein Blue, AM</a></td><td><a href="/order/catalog/product/C34852">Calcein, AM, cell-permeant dye</a></td><td> </td><td> </td><td> </td><td>Short-term (minutes to hours) </td></tr><tr><td rowspan="4"><a href="/us/en/home/life-science/cell-analysis/cell-viability-and-regulation/cell-viability.html"><b>Viability (dead cell indicators)</b></a></td><td> </td><td><a href="/order/catalog/product/S7020">SYTOX Green Nucleic Acid Stain</a></td><td><a href="/order/catalog/product/S11368">SYTOX Orange Nucleic Acid Stain</a></td><td> </td><td><a href="/order/catalog/product/S11380">SYTOX Deep Red Nucleic Acid Stain</a></td><td rowspan="4">Short- to long-term (24+ hours)</td></tr><tr><td> </td><td><a href="/order/catalog/product/R37109">NucGreen Dead 488 ReadyProbes Reagent (SYTOX Green)</a></td><td> </td><td> </td><td> </td></tr><tr><td> </td><td><a href="/order/catalog/product/Y3601">YOYO-1 Iodide</a></td><td> </td><td><a href="/order/catalog/product/Y3606">YOYO-3 Iodide</a></td><td> </td></tr><tr><td> </td><td><a href="/order/catalog/product/I10291">Image-iT DEAD Green Viability Stain</a></td><td> </td><td> </td><td> </td></tr><tr><td><a href="/sg/en/home/life-science/cell-analysis/cell-viability-and-regulation/cell-viability.html"><b>Viability (viability/cytotoxicity kits)</b></a></td><td> </td><td><a href="/order/catalog/product/L32250">LIVE/DEAD Viability/Cytotoxicity Assay Kit (Green/Deep Red)</a></td><td> </td><td> </td><td><a href="/order/catalog/product/L32250">LIVE/DEAD Viability/Cytotoxicity Assay Kit (Green/Deep Red)</a></td><td>Short-term (minutes to hours) </td></tr><tr><td><a href="/sg/en/home/life-science/cell-analysis/cell-structure/celllight-ready-to-use-fluorescent-protein-based-reagents.html"><b>Fluorescent protein–based cell structure reagents</b></a></td><td> </td><td><a href="/sg/en/home/life-science/cell-analysis/cell-structure/celllight-ready-to-use-fluorescent-protein-based-reagents.html#prd1">CellLight GFP BacMam 2.0</a></td><td><a href="/sg/en/home/life-science/cell-analysis/cell-structure/celllight-ready-to-use-fluorescent-protein-based-reagents.html#prd1">CellLight RFP BacMam 2.0</a></td><td> </td><td> </td><td>Long-term (overnight to days/2-3 generations)</td></tr></tbody></table></div></div><div class="heading section"><h2 id="environmental-control"> How to maintain environmental control during live-cell imaging </h2></div><div class="text parbase section"><p>Fluorescence imaging platforms combined with incubators provides a controlled and stable environment that allows long-term imaging of cells in their natural state. The <a></a><a href="/sg/en/home/life-science/cell-analysis/cellular-imaging/evos-cell-imaging-systems/accessories/onstage-incubator.html">EVOS Onstage Incubator</a> for the EVOS M7000 and M5000 Cell Imaging Systems and the <a></a><a href="/sg/en/home/life-science/cell-analysis/cellular-imaging/evos-cell-imaging-systems/accessories/onstage-incubator.html">Invitrogen HCA Onstage Incubator</a> for automated high-content screening and analysis on the CellInsight HCS/HCA platforms enable time-lapse and kinetic imaging and analysis of live cells within an environmental chamber. When performing long-term live-cell imaging within an incubated environment, reagents should be used in traditional cell culture medium or <a></a><a href="/order/catalog/product/A1896701">FluoroBrite DMEM (Cat. No. A1896701)</a> buffered with bicarbonate to maintain physiological pH in a 5% CO<sub>2 </sub>environment.</p></div><div class="heading section"><h2 id="apoptosis"> Example: apoptosis in live-cell imaging experiments </h2></div><div class="text parbase section"><p>Cellular apoptosis is a tightly controlled programmed cell death process used to remove excess, damaged, and unneeded cells or tissues. Apoptosis can be detected through caspase activation, DNA fragmentation, disruption in active mitochondria, and plasma membrane changes. The <a></a><a href="/order/catalog/product/C10423">CellEvent Caspase 3/7 reagents</a> are fluorogenic caspase substrates that detect caspase activation to assay for apoptosis in live cells. These reagents do not require wash steps and can be used for real-time and time-lapse live-cell imaging of apoptosis for 48 hours or longer.</p></div><div class="image parbase section"><div id="cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsysimage_c5a4"><a class="image-enlarge-link" data-lightbox="image_c5a4" href="/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/_jcr_content/MainParsys/image_c5a4/foregroundimg.img.jpg/1738165968964.jpg"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_c5a4/foregroundimg.img.320.medium.jpg/1738165968946.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_c5a4/foregroundimg.img.480.high.jpg/1738165968946.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_c5a4/foregroundimg.img.620.high.jpg/1738165968946.jpg' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_c5a4/foregroundimg.img.full.high.jpg/1738165968946.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_c5a4/foregroundimg.img.320.high.jpg/1738165968946.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_c5a4/foregroundimg.img.480.high.jpg/1738165968946.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_c5a4/foregroundimg.img.320.medium.jpg/1738165968946.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_c5a4/foregroundimg.img.620.high.jpg/1738165968946.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_c5a4/foregroundimg.img.320.low.jpg/1738165968946.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_c5a4/foregroundimg.img.480.medium.jpg/1738165968946.jpg" alt='Cartoon of CellEvent Caspase 3/7 workflow' title='CellEvent Caspase 3/7 workflow'></picture><style> #cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsysimage_c5a4 img{ width:100%; max-width: 648px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsysimage_c5a4 .image-contain" + "," + "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsysimage_c5a4 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a></div></div><div class="text parbase section"><p><small><b>Figure 1. Apoptosis live-cell endpoint assay workflow using the <a></a><a href="/order/catalog/product/C10432">CellEvent Caspase-3/7 Green Detection Reagent (Cat. No. C10432)</a>.</b> Seed cells and follow with treatment.  Add diluted CellEvent Caspase-3/7 Green Detection Reagent to cells. CellEvent Caspase 3/7 dyes may be imaged directly on cells in complete media without wash. Measure fluorescence starting as early as 30 minutes to as long as 72 hours.</small></p><p><a class="btn btn-primary" href="https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0028521-CellEventCaspase3-7DetectionReagent-UG.pdf" target="_blank">Download Caspase 3/7 Protocol</a></p><p> </p></div><div class="image parbase section"><div id="cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsysimage_ccb5"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_ccb5/foregroundimg.img.320.low.jpg/1738165968986.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_ccb5/foregroundimg.img.320.medium.jpg/1738165968986.jpg' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_ccb5/foregroundimg.img.480.medium.jpg/1738165968986.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_ccb5/foregroundimg.img.620.high.jpg/1738165968986.jpg' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_ccb5/foregroundimg.img.full.high.jpg/1738165968986.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_ccb5/foregroundimg.img.320.high.jpg/1738165968986.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_ccb5/foregroundimg.img.480.high.jpg/1738165968986.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_ccb5/foregroundimg.img.320.medium.jpg/1738165968986.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_ccb5/foregroundimg.img.620.high.jpg/1738165968986.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_ccb5/foregroundimg.img.320.low.jpg/1738165968986.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_ccb5/foregroundimg.img.480.medium.jpg/1738165968986.jpg" alt='Cells stained with CellEvent Caspase 3/7 Green as indicated with apoptotic cells being bright green and live cells being dark, and imaged with Incucyte® ZOOM Live-Cell Analysis' title='Cells stained with CellEvent Caspase 3/7 Green and imaged with Incucyte® ZOOM Live-Cell Analysis'></picture><style> #cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsysimage_ccb5 img{ width:100%; max-width: 900px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsysimage_ccb5 .image-contain" + "," + "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsysimage_ccb5 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div><div class="text parbase section"><p><small><b>Figure 2. Example of Live Cell Imaging of apoptotic cells stained with CellEvent Caspase 3/7 Green.</b> HuVEC cells treated with a cocktail stained with <a></a><a href="/order/catalog/product/C10432">CellEvent Caspase 3/7 Green Assay (Cat. No. C10432)</a>  and imaged with Incucyte® ZOOM Live-Cell Analysis System at 0, 24, 48, and 72 hours after treatment. (Image from: Sambi M, Samuel V, Qorri B, Haq S, Burov SV, Markvicheva E, Harless W, Szewczuk MR. A Triple Combination of Metformin, Acetylsalicylic Acid, and Oseltamivir Phosphate Impacts Tumour Spheroid Viability and Upends Chemoresistance in Triple-Negative Breast Cancer. Drug Des Devel Ther. 2020;14:1995-2019 PMID: <a href="https://pubmed.ncbi.nlm.nih.gov/32546966/" target="_blank">32546966</a><span class="icon icon-outbound"></span>)</small></p></div><div class="productlist parbase section"><div id="skulist" style="display:none">C10430,C10432</div><div id='ltajax-4896944466795801568'></div><script type="text/javascript">(function() {$('#ltajax-4896944466795801568').load('/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/productlist_9463.ajax.html?skulist=');})(); </script></div><div class="heading section"><h2 id="mitochondrial"> Example: mitochondrial activity in live-cell imaging experiments </h2></div><div class="text parbase section"><p>Live-cell imaging reagents include both cell structure reagents used to identify cellular components and cell function reagents to analyze cellular functions and processes. For instance, MitoTracker dyes accumulate in active mitochondria in live cells and covalently attach to mitochondria to allow evaluation of mitochondrial localization and abundance. In contrast, <a href="/order/catalog/product/I34361">TMRM</a> and <a href="/order/catalog/product/T669">TMRE</a> are dynamic mitochondria membrane potential indicator dyes that fluctuate in and out of the mitochondria based on membrane potential.</p></div><div class="image parbase section"><div id="cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsysimage_f6c8"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_f6c8/foregroundimg.img.320.low.jpg/1738165969053.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_f6c8/foregroundimg.img.320.medium.jpg/1738165969053.jpg' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_f6c8/foregroundimg.img.480.medium.jpg/1738165969053.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_f6c8/foregroundimg.img.620.high.jpg/1738165969053.jpg' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_f6c8/foregroundimg.img.full.high.jpg/1738165969053.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_f6c8/foregroundimg.img.320.high.jpg/1738165969053.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_f6c8/foregroundimg.img.480.high.jpg/1738165969053.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_f6c8/foregroundimg.img.320.medium.jpg/1738165969053.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_f6c8/foregroundimg.img.620.high.jpg/1738165969053.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_f6c8/foregroundimg.img.320.low.jpg/1738165969053.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_f6c8/foregroundimg.img.480.medium.jpg/1738165969053.jpg" alt='Cells stained with MitoTracker Red as indicated with cells with active mitochondria being bright red, the dead cells are stained in green, the nucleus in blue, and imaged with Incucyte® ZOOM Live-Cell Analysis' title='Cells stained with MitoTracker Red and imaged with Incucyte® ZOOM Live-Cell Analysis'></picture><style> #cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsysimage_f6c8 img{ width:100%; max-width: 900px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsysimage_f6c8 .image-contain" + "," + "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsysimage_f6c8 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div><div class="text parbase section"><p><small><b>Figure 3. Monitoring active mitochondria in live cells.</b> Fibroblast lines from patients were cultured and stained with <a href="/order/catalog/product/M22426"></a><a>MitoTracker Red (Cat. No. </a>M22426), <a href="/order/catalog/product/I10291"></a><a>Image-iT DEAD Green dyes (Cat. No. </a>I10291), <a href="/order/catalog/product/R37165"></a><a>Hoechst (Cat. No. </a>R37165) and imaged on an Incucyte® ZOOM Live-Cell Analysis System. (Image from Smith GA, Jansson J, Rocha EM, Osborn T, Hllett PJ, Isacson O. Fibroblast Biomarkers of Sporadic Parkinson's Disease and LRRK2 Kinase Inhibition. Mol Neurobiol. 2016 Oct;53(8):5161-77. PMCID: <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5012155/">PMC5012155</a>. <span class="icon icon-outbound"></span>)</small></p></div><div class="productlist parbase section"><div id="skulist" style="display:none">M7514,M22426,M7512,T668,T669</div><div id='ltajax-9017941890016552893'></div><script type="text/javascript">(function() {$('#ltajax-9017941890016552893').load('/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/productlist_350f.ajax.html?skulist=');})(); </script></div><div class="divider section"><hr /></div><div class="heading section"><h2 id="imaging-experiments"> Example: viability in live-cell imaging experiments </h2></div><div class="text parbase section"><p>Cell impermeant nucleic acid stains can be used as dead cell indicators in live-cell imaging experiments. Nucleic acid stains such as YOYO-1 and YOYO-3 are nontoxic dyes that have bright signals and large fluorescence enhancement upon binding to DNA. These dyes are impermeant to viable cells and selectively stain dead cells with compromised membranes to enable live-cell imaging over time. </p></div><div class="image parbase section"><div id="cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsysimage_83ef"><a class="image-enlarge-link" data-lightbox="image_83ef" href="/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/_jcr_content/MainParsys/image_83ef/foregroundimg.img.jpg/1738165969139.jpg"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_83ef/foregroundimg.img.320.medium.jpg/1738165969124.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_83ef/foregroundimg.img.480.high.jpg/1738165969124.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_83ef/foregroundimg.img.620.high.jpg/1738165969124.jpg' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_83ef/foregroundimg.img.full.high.jpg/1738165969124.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_83ef/foregroundimg.img.320.high.jpg/1738165969124.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_83ef/foregroundimg.img.480.high.jpg/1738165969124.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_83ef/foregroundimg.img.320.medium.jpg/1738165969124.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_83ef/foregroundimg.img.620.high.jpg/1738165969124.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_83ef/foregroundimg.img.320.low.jpg/1738165969124.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_83ef/foregroundimg.img.480.medium.jpg/1738165969124.jpg" alt='Charts and fluorescent images of YOYO-1 and YOYO-3 data' title='YOYO-1 and YOYO-3 dye data'></picture><style> #cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsysimage_83ef img{ width:100%; max-width: 757px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsysimage_83ef .image-contain" + "," + "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsysimage_83ef .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a></div></div><div class="text parbase section"><small><b>Figure 4. Monitoring cell viability with YOYO-3.</b> Mouse embryonic fibroblasts (MEF) were treated with cycloheximide (CHX) and stained with YOYO-3, then scanned at 2-hour intervals for up to 24 hours on the Incucyte® ZOOM Live-Cell Analysis System to monitor cell viability based on dead cell staining. Annexin V and CellEvent Caspase 3/7 Green were also used to detect apoptotic cells. (Image from Gelles JD, Chipuk JE. Robust high-throughput kinetic analysis of apoptosis with real-time high-content live-cell imaging. Cell Death Dis. 2016 Dec 1;7(12):e2493. <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5261025/">doi: 10.1038/cddis.2016.332</a>. Erratum in: Cell Death Dis. 2017 May 4;8(5):e2758. PMCID: <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5261025/">PMC5261025</a>.)</small></div><div class="divider section"><hr /></div><div class="heading section"><h2 id="detect-changes"> Tips to detect changes in live-cell experiments </h2></div><div class="tabworkflowcontainer section"><div class="tabbable tabs-workflow workflow-5steps"><ul class="nav nav-workflow"><li class="first-tab active"><a data-toggle="tab" href="#container-0d3730df-2531-4439-a5db-7f16cbd279d2"><b><span><span>Step 1</span></span></b><strong>Plan</strong></a></li><li class="next-tab"><a data-toggle="tab" href="#container-a8c58e24-0696-4024-b994-eec4b59254ef"><b><span><span>Step 2</span></span></b><strong>Culture</strong></a></li><li><a data-toggle="tab" href="#container-b14a8763-2978-4940-9089-7618b2c4f1f9"><b><span><span>Step 3</span></span></b><strong>Label</strong></a></li><li><a data-toggle="tab" href="#container-755e4acd-6798-4e66-8837-5047e5c51b9f"><b><span><span>Step 4</span></span></b><strong>Optimize</strong></a></li><li class="last-tab"><a data-toggle="tab" href="#container-53ca6743-86fd-4cc8-a20f-7905fcf841bf"><b><span><span>Step 5</span></span></b><strong>Image</strong></a></li></ul><div class="tab-content"><div class="tab-pane active" id="container-0d3730df-2531-4439-a5db-7f16cbd279d2"><div class="container-0d3730df-2531-4439-a5db-7f16cbd279d2 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h2> Step 1: Plan </h2></div><div class="heading section"><h3> Design your experiment with careful consideration of the tools and resources needed for each step. </h3></div><div class="text parbase section"><h4>Advantages</h4><ul><li>Observe dynamic cellular processes as they happen</li><li>Study and image several processes and functions simultaneously using multiplexed assays</li><li>Study cellular structures in their native environment, resulting in more realistic results closer to in vivo scenarios</li><li>Track cellular biomolecules and structures over time</li><li>Observe interactions between cells</li><li>Cellular enzymes and other cytosolic biomolecules remain in the cell</li></ul></div><div class="text parbase section"><h4>Considerations</h4><ul><li>Must have a specific way to label your target with minimal toxicity – whether it is a molecule, a cellular function, or a cellular state</li><li>Living cells are generally not permeable to large detection molecules such as antibodies</li><li>Moving objects can be more difficult to keep in focus</li><li>Certain techniques can be harmful to living cells</li><li>Cells must be kept in their natural physiological state</li></ul></div><div class="well-cmp well-default well-blue-light section"><div class="well well-blue-light well-default"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><h3> Resource highlights </h3></div><div class="text parbase section"><ul class="unstyled"><li><a href="/sg/en/home/life-science/cell-analysis/cell-analysis-learning-center/cell-imaging-information.html">Learning Center: cell imaging</a></li><li><a href="/sg/en/home/life-science/cell-analysis/cell-analysis-learning-center/molecular-probes-school-of-fluorescence/imaging-basics.html">Molecular Probes School of Fluorescence: imaging basics</a></li><li><a href="/order/stain-it/#!/">Cell staining tool</a></li><li><a href="/order/fluorescence-spectraviewer#!/">Fluorescence SpectraViewer</a></li><li><a href="/sg/en/home/technical-resources/technical-reference-library/cell-analysis-support-center/cell-imaging-support.html">Cell imaging support</a></li><li><a href="/sg/en/home/technical-resources/contact-us.html">Contact us</a></li></ul></div></div></div></div></div></div></div><div class="end"></div></div></div><div class="tab-pane" id="container-a8c58e24-0696-4024-b994-eec4b59254ef"><div class="container-a8c58e24-0696-4024-b994-eec4b59254ef tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h2> Step 2: Culture </h2></div><div class="heading section"><h3> Maintain or grow your cells in optimum conditions. </h3></div><div class="text parbase section"><p>Keeping cells alive and healthy during various experimental manipulations, detection, and imaging is no small task. The choice of medium is particularly important for time-lapse imaging and experiments where cells are exposed to ambient conditions for longer periods. For reliable results with live cells, it is essential that the cells be healthy and kept in an environment as close as possible to physiological temperature, pH, oxygen level, and other conditions.</p><p><a class="cta-link" href="/sg/en/home/references/gibco-cell-culture-basics.html">Learn more about cell culture</a></p></div><div class="well-cmp well-default well-blue-light section"><div class="well well-blue-light well-default"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><h3> Product highlights </h3></div><div class="text parbase section"><p>These media and wash buffers are created specifically for live-cell imaging and detection. Employing them in your experiments can help you improve image clarity, reduce background fluorescence, and optimize cell viability.</p><ul><li><b><a href="/sg/en/home/life-science/cell-culture/cell-culture-plastics.html">Thermo Scientific Nunc cell culture vessels with Nunclon Delta surface treatment</a></b> have been validated with Gibco media to confirm consistent cell growth across multiple cell lines. It’s a proven combination for happy cells and happy scientists.</li><li><b><a href="/sg/en/home/life-science/cell-culture/organoids-spheroids-3d-cell-culture/extracellular-matrices-ecm.html">Gibco extracellular matrices, scaffolds, and proteins</a></b> provide in vivo–like morphology and physiologically relevant environments for more realistic cell biology and better intercellular interactions.</li><li><b><a href="/sg/en/home/life-science/cell-analysis/cell-analysis-instruments/automated-cell-counters.html">Invitrogen Countess 3 FL Automated Cell Counter</a></b> is an affordable and automated tool for checking the health of your cells quickly and objectively. It has reusable or disposable slide options.</li></ul></div></div></div></div></div></div><div class="text parbase section"><div class="table-component-wrapper"><table style="background-color: #fcf8e3;" cellspacing="0" cellpadding="10" border="0" id="table-2123960591"><tbody><tr><td><h3>Tips</h3><p>You can improve image clarity, reduce background fluorescence, and optimize cell viability by using media and wash buffers created specifically for live-cell imaging and detection. <a href="#prodselect" class="cta-link">See product selection guide</a></p></td></tr></tbody></table></div></div></div><div class="end"></div></div></div><div class="tab-pane" id="container-b14a8763-2978-4940-9089-7618b2c4f1f9"><div class="container-b14a8763-2978-4940-9089-7618b2c4f1f9 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h2> Step 3: Label </h2></div><div class="heading section"><h3> Target cell structures, cell functions, and proteins of interest with selective dyes and stains. </h3></div><div class="text parbase section"><p>The appropriate fluorophore (targeted fluorescent protein or small membrane-permeant reagent) should be used to monitor your target cellular structure or process. Additional fluorophores can be used to monitor multiple cellular structures and processes, but the excitation and emission spectra should be checked using the <a href="/order/fluorescence-spectraviewer#!/">Fluorescence SpectraViewer</a> or <a href="/order/stain-it/#!/">Stain-iT Cell Staining Simulator</a> to ensure minimal spectra overlap. It is critical to avoid using too much fluorescent label because excessive fluorescent labeling can result in:</p><ul><li>Nonspecific staining with increased background signals</li><li>Physiological artifacts and structural perturbations</li><li>Cytotoxicity</li><li>Spectral overlap</li></ul><h4>Note that:</h4><ul><li>Live-cell structure reagents—help identify cellular components</li><li>Live-cell function reagents—help identify cellular functions and processes</li></ul></div><div class="well-cmp well-default well-blue-light section"><div class="well well-blue-light well-default"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><h3> Product highlights </h3></div><div class="text parbase section"><ul><li><b><a href="/sg/en/home/life-science/cell-analysis/cell-structure/celllight-ready-to-use-fluorescent-protein-based-reagents.html">Invitrogen CellLight reagents</a></b> have proven to be the easiest to use for labeling specific structures in live cells. Targeted fluorescent proteins are introduced using the Invitrogen BacMam transduction system; no molecular biology techniques are required. Simply add the reagent to your cells, incubate overnight, and you’re ready to image in the morning.</li><li><b><a href="/sg/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology.html">Invitrogen CellTracker reagents</a></b> are a diverse reagent class used for labeling mammalian cells to view changes in morphology or location. These nontoxic fluorescent dyes are designed to freely pass through cell membranes into cells, where they are transformed into cell-impermeant reaction products. Incubating cells with a CellTracker reagent for 30 minutes will provide at least 72 hours of fluorescent signal (typically three to six generations).</li><li><b><a href="/sg/en/home/brands/molecular-probes/key-molecular-probes-products/phrodo-indicators.html">Invitrogen pHrodo indicators</a></b> are fluorogenic dyes that dramatically increase in fluorescence as the pH of their surroundings becomes more acidic. When conjugated to dextrans, proteins, or other particles, pHrodo dyes can be used as highly specific sensors of endocytic and phagocytic internalization and lysosomal sequestration in live cells, offering a superior alternative to conjugates of other fluorescent dyes such as fluorescein and tetramethylrhodamine.</li></ul></div></div></div></div></div></div><div class="text parbase section"><div class="table-component-wrapper"><table style="background-color: #fcf8e3;" cellspacing="0" cellpadding="10" border="0" id="table-1311655089"><tbody><tr><td><h3>Tips</h3><ul><li>Consider using a longer wavelength fluorescent reagent if extended light exposure is required. This will require lower excitation power, which can correlate to lower phototoxicity and healthier cells.</li><li>Staining must be optimized for the particular assay readout, spectral compatibility, and signal-to-background ratio.</li><li>Removing the labeling solution and rinsing with fresh medium will reduce background fluorescence.</li></ul></td></tr></tbody></table></div></div></div><div class="end"></div></div></div><div class="tab-pane" id="container-755e4acd-6798-4e66-8837-5047e5c51b9f"><div class="container-755e4acd-6798-4e66-8837-5047e5c51b9f tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h2> Step 4: Optimize </h2></div><div class="heading section"><h3> Minimize background and maintain photostability of fluorescence signals. </h3></div><div class="text parbase section"><p>Signal-to-background ratio can be optimized by using reagents that reduce extracellular fluorescence and increase fluorophore photostability. It is important to image in media that have been specifically designed for maintaining cell health while reducing or eliminating background fluorescence in live-cell imaging experiments (see <b>Table 1</b>). The addition of a background suppressor compatible with live cells can also help reduce extracellular background fluorescence and eliminate the need for a wash step. <a href="/order/catalog/product/P36975">ProLong Live Antifade Reagent</a> can be applied to samples to reduce photobleaching of fluorophores, preventing signal loss with multiple or long exposures.</p></div><div class="text parbase section"><p><b>Table 1. Imaging media comparison.</b></p><div class="table-component-wrapper"><table class="rte-table" id="table--1907194242"><tbody><tr><th>Reagent</th><th>Cell washing</th><th>Short-term imaging</th><th>Imaging up to 4 hours</th><th>Long-term imaging</th></tr><tr><td><b><a href="/order/catalog/product/10010023">Gibco PBS, pH 7.4</a></b></td><td style="text-align: center;"><img src="/content/dam/LifeTech/migration/images/products-services/cvc.par.97777.image.24.24.1..gif"></td><td style="text-align: center;"><img src="/content/dam/LifeTech/migration/images/products-services/cvc.par.97777.image.24.24.1..gif"></td><td> </td><td> </td></tr><tr><td><b><a href="/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cell-culture-media/dmem.html">Gibco FluoroBrite DMEM</a></b></td><td style="text-align: center;"><img src="/content/dam/LifeTech/migration/images/products-services/cvc.par.97777.image.24.24.1..gif"></td><td style="text-align: center;"><img src="/content/dam/LifeTech/migration/images/products-services/cvc.par.97777.image.24.24.1..gif"></td><td style="text-align: center;"><img src="/content/dam/LifeTech/migration/images/products-services/cvc.par.97777.image.24.24.1..gif"></td><td style="text-align: center;"><img src="/content/dam/LifeTech/migration/images/products-services/cvc.par.97777.image.24.24.1..gif"></td></tr><tr><td><a href="/order/catalog/product/A59688DJ?SID=srch-srp-A59688DJ"><b>Invitrogen Live Cell Imaging Solution</b></a></td><td style="text-align: center;"><img src="/content/dam/LifeTech/migration/images/products-services/cvc.par.97777.image.24.24.1..gif"></td><td style="text-align: center;"><img src="/content/dam/LifeTech/migration/images/products-services/cvc.par.97777.image.24.24.1..gif"></td><td style="text-align: center;"><img src="/content/dam/LifeTech/migration/images/products-services/cvc.par.97777.image.24.24.1..gif"></td><td> </td></tr></tbody></table></div></div><div class="well-cmp well-default well-blue-light section"><div class="well well-blue-light well-default"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><h3> Product highlights </h3></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="text parbase section"><ul><li><a href="/order/catalog/product/B10512">Invitrogen BackDrop Background Suppressor</a> is used when observing high background signal or weak fluorescence in the blue, green, or red channels.</li><li><a href="/order/catalog/product/10010023">Gibco PBS, pH 7.4</a> is ideal for cell washing and short-term imaging where prolonged incubation is not required.</li><li><a href="/order/catalog/product/A59688DJ?SID=srch-srp-A59688DJ">Invitrogen Live Cell Imaging Solution</a> is an optically clear solution used for imaging, dye loading, and wash steps. It helps keep cells healthy for up to 4 hours.</li></ul></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="image parbase section"><div id="cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-755e4acd-6798-4e66-8837-5047e5c51b9ftabwell_1769uiparimage_7f14"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_7f14/foregroundimg.img.full.high.jpg/1738165969838.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_7f14/foregroundimg.img.full.high.jpg/1738165969838.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_7f14/foregroundimg.img.320.high.jpg/1738165969838.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_7f14/foregroundimg.img.480.high.jpg/1738165969838.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_7f14/foregroundimg.img.320.medium.jpg/1738165969838.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_7f14/foregroundimg.img.620.high.jpg/1738165969838.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_7f14/foregroundimg.img.320.low.jpg/1738165969838.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_7f14/foregroundimg.img.480.medium.jpg/1738165969838.jpg" alt='Invitrogen BackDrop Background Suppressor imaging results' title='Invitrogen BackDrop Background Suppressor imaging results'></picture><style> #cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-755e4acd-6798-4e66-8837-5047e5c51b9ftabwell_1769uiparimage_7f14 img{ width:100%; max-width: 450px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-755e4acd-6798-4e66-8837-5047e5c51b9ftabwell_1769uiparimage_7f14 .image-contain" + "," + "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-755e4acd-6798-4e66-8837-5047e5c51b9ftabwell_1769uiparimage_7f14 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script><div class="caption"><small><p><b>Live HeLa cells labeled with <a href="/order/catalog/product/T34075">Tubulin Tracker Green dye</a> and <a href="/order/catalog/product/T34076">Tubulin Tracker Deep Red dye</a>.</b> Both labels show high off-cell background when the probe is left in the staining solution (left). Addition of <a href="/order/catalog/product/B10512">BackDrop Background Suppressor</a> greatly reduces extracellular background while leaving intracellular labeling unaffected (right), thus enabling a no-wash protocol for high-contrast imaging of tubulin in live cells.</p></small></div></div></div></div></div></div><div style="clear:both"></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-755e4acd-6798-4e66-8837-5047e5c51b9ftabwell_1769uiparimage_b4a1"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_b4a1/foregroundimg.img.full.high.jpg/1738165969885.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_b4a1/foregroundimg.img.full.high.jpg/1738165969885.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_b4a1/foregroundimg.img.320.high.jpg/1738165969885.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_b4a1/foregroundimg.img.480.high.jpg/1738165969885.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_b4a1/foregroundimg.img.320.medium.jpg/1738165969885.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_b4a1/foregroundimg.img.620.high.jpg/1738165969885.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_b4a1/foregroundimg.img.320.low.jpg/1738165969885.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_b4a1/foregroundimg.img.480.medium.jpg/1738165969885.jpg" alt='Invitrogen ProLong Live Reagent confocal imaging results' title='Invitrogen ProLong Live Reagent confocal imaging results'></picture><style> #cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-755e4acd-6798-4e66-8837-5047e5c51b9ftabwell_1769uiparimage_b4a1 img{ width:100%; max-width: 450px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-755e4acd-6798-4e66-8837-5047e5c51b9ftabwell_1769uiparimage_b4a1 .image-contain" + "," + "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-755e4acd-6798-4e66-8837-5047e5c51b9ftabwell_1769uiparimage_b4a1 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script><div class="caption"><small><p class="muted"><b>The overall signal protection offered by <a href="/order/catalog/product/P36974">ProLong Live reagent</a></b>compared to untreated samples is calculated based on the scan number where treated and untreated samples reach the EC50 value. The addition of ProLong Live reagent permitted 100% more captures with <a href="/order/catalog/product/C10601">Invitrogen CellLight Mitochondria-RFP reagent</a>.</p></small></div></div></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="image parbase section"><div id="cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-755e4acd-6798-4e66-8837-5047e5c51b9ftabwell_1769uiparimage_aee7"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_aee7/foregroundimg.img.full.high.jpg/1738165969926.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_aee7/foregroundimg.img.full.high.jpg/1738165969926.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_aee7/foregroundimg.img.320.high.jpg/1738165969926.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_aee7/foregroundimg.img.480.high.jpg/1738165969926.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_aee7/foregroundimg.img.320.medium.jpg/1738165969926.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_aee7/foregroundimg.img.620.high.jpg/1738165969926.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_aee7/foregroundimg.img.320.low.jpg/1738165969926.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_aee7/foregroundimg.img.480.medium.jpg/1738165969926.jpg" alt='Invitrogen ProLong Live Reagent fluorescence imaging' title='Invitrogen ProLong Live Reagent fluorescence imaging'></picture><style> #cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-755e4acd-6798-4e66-8837-5047e5c51b9ftabwell_1769uiparimage_aee7 img{ width:100%; max-width: 450px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-755e4acd-6798-4e66-8837-5047e5c51b9ftabwell_1769uiparimage_aee7 .image-contain" + "," + "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-755e4acd-6798-4e66-8837-5047e5c51b9ftabwell_1769uiparimage_aee7 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script><div class="caption"><small><p class="muted"><b>After 120 exposures using a standard time-lapse imaging protocol,</b> samples treated with <a href="/order/catalog/product/P36974">ProLong Live reagent</a> are >20% brighter than untreated cells, enabling more data collection time.</p></small></div></div></div></div></div></div><div style="clear:both"></div></div></div></div></div></div><div class="text parbase section"><div class="table-component-wrapper"><table style="background-color: #fcf8e3;" cellspacing="0" cellpadding="10" border="0" id="table-360779586"><tbody><tr><td><h3>Tips</h3><ul><li>If no further culture is planned, a background suppressor can be used to optimize the signal by reducing the haze and increasing the contrast.</li><li>The use of an antifade reagent has been shown to increase fluorophore photostability and decrease the effect of phototoxicity in a variety of sample types.</li></ul></td></tr></tbody></table></div></div></div><div class="end"></div></div></div><div class="tab-pane" id="container-53ca6743-86fd-4cc8-a20f-7905fcf841bf"><div class="container-53ca6743-86fd-4cc8-a20f-7905fcf841bf tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h2> Step 5: Image </h2></div><div class="heading section"><h3> Live-cell imaging of dynamic processes requires active observation over time </h3></div><div class="text parbase section"><h4>Illumination and detection</h4><p>To minimize phototoxicity, choose imaging systems that give you the greatest control of light sources. Try to minimize light intensity, exposure time, wavelength range, and amount of excitation energy for illuminating your cells while still generating a good signal with low background. Use the illumination that gives you the highest signal with the lowest level of fluorophore excitation. In some cases (particularly when you wish to image over a long period of time), it is advisable to sacrifice resolution by using shorter exposure times or lower magnification in exchange for healthier cells.</p><p>Live-cell imaging over longer periods of time can be challenging because the target may move out of focus during the course of the experiment. Many microscopes have autofocusing features that can help keep your target in focus longer and reduce focal drift. Additionally, maintaining cells at a constant temperature and keeping the volume of solution in the vessel constant will help with focal drift.</p><h4>Environmental control</h4><p>Many cells cannot tolerate deviations from their optimal temperature, osmolarity, pH, and humidity. Requirements vary depending on what experimental question you are asking. For example, experiments investigating cell growth and division may have a different set of requirements than experiments involving receptor activation and calcium accumulation. Some robust immortalized cell lines will tolerate being imaged or monitored for short periods of time without any environmental control. Conversely, for long-term imaging and detection studies, good results with both immortalized cells and primary cells typically require tightly controlled environmental parameters.</p></div><div class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div class="image parbase section"><div id="cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-53ca6743-86fd-4cc8-a20f-7905fcf841bftabimage_12db"><a class="image-enlarge-link" data-lightbox="image_12db" href="/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/_jcr_content/MainParsys/tabworkflowcontainer_0/container-53ca6743-86fd-4cc8-a20f-7905fcf841bf/tab/image_12db/backgroundimg.img.jpg/1738165970099.jpg"><picture><source 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alt='HDFn cells loaded with CellTracker Deep Red Dye' title='HDFn cells loaded with CellTracker Deep Red Dye'></picture><style> #cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-53ca6743-86fd-4cc8-a20f-7905fcf841bftabimage_12db img{ width:100%; max-width: 1050px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-53ca6743-86fd-4cc8-a20f-7905fcf841bftabimage_12db .image-contain" + "," + "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-53ca6743-86fd-4cc8-a20f-7905fcf841bftabimage_12db .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a><div class="caption"><small><span class=" icon-zoom-in"> </span>Click image to enlarge</small></div></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div class="text parbase section"><p><small class="muted"><b>A scratch wound in a culture of HDFn cells loaded with <a href="/order/catalog/product/C34565">Invitrogen CellTracker Deep Red Dye</a>.</b> (<b>A</b>) The illuminated area was subjected to repeated illumination for 10 hours. Cells in this area show signs of phototoxicity (a loss of viability as cells were not able to grow into the wound). (<b>B</b>) Cells in the non-illuminated area show viable cell growth into the wound.</small></p></div></div></div><div style="clear:both"></div><div class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div class="image parbase section"><div id="cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-53ca6743-86fd-4cc8-a20f-7905fcf841bftabimage_fd1f"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-53ca6743-86fd-4cc8-a20f-7905fcf841bf/tab/image_fd1f/foregroundimg.img.full.high.jpg/1738165970134.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-53ca6743-86fd-4cc8-a20f-7905fcf841bf/tab/image_fd1f/foregroundimg.img.full.high.jpg/1738165970134.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-53ca6743-86fd-4cc8-a20f-7905fcf841bf/tab/image_fd1f/foregroundimg.img.320.high.jpg/1738165970134.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-53ca6743-86fd-4cc8-a20f-7905fcf841bf/tab/image_fd1f/foregroundimg.img.480.high.jpg/1738165970134.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-53ca6743-86fd-4cc8-a20f-7905fcf841bf/tab/image_fd1f/foregroundimg.img.320.medium.jpg/1738165970134.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-53ca6743-86fd-4cc8-a20f-7905fcf841bf/tab/image_fd1f/foregroundimg.img.620.high.jpg/1738165970134.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-53ca6743-86fd-4cc8-a20f-7905fcf841bf/tab/image_fd1f/foregroundimg.img.320.low.jpg/1738165970134.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-53ca6743-86fd-4cc8-a20f-7905fcf841bf/tab/image_fd1f/foregroundimg.img.480.medium.jpg/1738165970134.jpg" alt='Catastrophic blebbing of the cell membrane' title='Catastrophic blebbing of the cell membrane'></picture><style> #cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-53ca6743-86fd-4cc8-a20f-7905fcf841bftabimage_fd1f img{ width:100%; max-width: 400px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-53ca6743-86fd-4cc8-a20f-7905fcf841bftabimage_fd1f .image-contain" + "," + "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-53ca6743-86fd-4cc8-a20f-7905fcf841bftabimage_fd1f .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div class="text parbase section"><p><small class="muted"><b>The top cell shows catastrophic blebbing of the cell membrane caused by excessive light exposure.</b> Blebbing is a term used to describe membrane perturbation caused by toxicity. By contrast, the bottom cell remains relatively healthy and is not displaying aberrant morphology.</small></p></div></div></div><div style="clear:both"></div><div class="well-cmp well-default well-blue-light section"><div class="well well-blue-light well-default"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><h3> Product highlights </h3></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt3"><div class="parsys_column cq-colctrl-lt3-c0"><div container-id="cq-colctrl-lt3" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-53ca6743-86fd-4cc8-a20f-7905fcf841bftabwell_60c9uiparimage_af9a"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-53ca6743-86fd-4cc8-a20f-7905fcf841bf/tab/well_60c9/uipar/image_af9a/foregroundimg.img.full.high.jpg/1738165970278.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-53ca6743-86fd-4cc8-a20f-7905fcf841bf/tab/well_60c9/uipar/image_af9a/foregroundimg.img.full.high.jpg/1738165970278.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-53ca6743-86fd-4cc8-a20f-7905fcf841bf/tab/well_60c9/uipar/image_af9a/foregroundimg.img.320.high.jpg/1738165970278.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-53ca6743-86fd-4cc8-a20f-7905fcf841bf/tab/well_60c9/uipar/image_af9a/foregroundimg.img.480.high.jpg/1738165970278.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-53ca6743-86fd-4cc8-a20f-7905fcf841bf/tab/well_60c9/uipar/image_af9a/foregroundimg.img.320.medium.jpg/1738165970278.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-53ca6743-86fd-4cc8-a20f-7905fcf841bf/tab/well_60c9/uipar/image_af9a/foregroundimg.img.620.high.jpg/1738165970278.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-53ca6743-86fd-4cc8-a20f-7905fcf841bf/tab/well_60c9/uipar/image_af9a/foregroundimg.img.320.low.jpg/1738165970278.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-53ca6743-86fd-4cc8-a20f-7905fcf841bf/tab/well_60c9/uipar/image_af9a/foregroundimg.img.480.medium.jpg/1738165970278.jpg" alt='Countess II FL Automated Cell Counter' title='Countess II FL Automated Cell Counter'></picture><style> #cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-53ca6743-86fd-4cc8-a20f-7905fcf841bftabwell_60c9uiparimage_af9a img{ width:100%; max-width: 230px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-53ca6743-86fd-4cc8-a20f-7905fcf841bftabwell_60c9uiparimage_af9a .image-contain" + "," + "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-53ca6743-86fd-4cc8-a20f-7905fcf841bftabwell_60c9uiparimage_af9a .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div><div container-id="cq-colctrl-lt3" comp-id="A2"><div class="heading section"><h4><a href="/sg/en/home/life-science/cell-analysis/cell-analysis-instruments/automated-cell-counters.html">Countess 3 FL Automated Cell Counter</a></h4></div></div><div container-id="cq-colctrl-lt3" comp-id="A3"><div class="text parbase section"><p>To avoid the pitfall of proceeding to the next step in your experiment with unhealthy cells, a quick check for cell health can be done on the <b><a href="/sg/en/home/life-science/cell-analysis/cell-analysis-instruments/automated-cell-counters.html">Countess 3 FL Automated Cell Counter</a></b> when used in conjunction with a variety of fluorescent reagents to detect cell viability, apoptosis, cytotoxicity, and transfection efficiency. The reusable slide option reduces consumption cost.</p></div></div></div><div class="parsys_column cq-colctrl-lt3-c1"><div container-id="cq-colctrl-lt3" comp-id="B1"><div class="image parbase section"><div id="cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-53ca6743-86fd-4cc8-a20f-7905fcf841bftabwell_60c9uiparimage_67b7"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-53ca6743-86fd-4cc8-a20f-7905fcf841bf/tab/well_60c9/uipar/image_67b7/foregroundimg.img.full.high.jpg/1738165970335.jpg' media="(min-width: 1px)"><img 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alt='Invitrogen EVOS M5000 Imaging System with EVOS Onstage Incubator' title='Invitrogen EVOS M5000 Imaging System with EVOS Onstage Incubator'></picture><style> #cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-53ca6743-86fd-4cc8-a20f-7905fcf841bftabwell_60c9uiparimage_67b7 img{ width:100%; max-width: 230px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-53ca6743-86fd-4cc8-a20f-7905fcf841bftabwell_60c9uiparimage_67b7 .image-contain" + "," + "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-53ca6743-86fd-4cc8-a20f-7905fcf841bftabwell_60c9uiparimage_67b7 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div><div container-id="cq-colctrl-lt3" comp-id="B2"><div class="heading section"><h4><a href="/sg/en/home/life-science/cell-analysis/cellular-imaging/evos-cell-imaging-systems/accessories/onstage-incubator.html">Invitrogen EVOS M5000 Imaging System with EVOS Onstage Incubator</a></h4></div></div><div container-id="cq-colctrl-lt3" comp-id="B3"><div class="text parbase section"><p>Designed specifically for <b><a href="/sg/en/home/life-science/cell-analysis/cellular-imaging/evos-cell-imaging-systems.html">Invitrogen EVOS imaging systems</a></b>, the <b><a href="/sg/en/home/life-science/cell-analysis/cellular-imaging/evos-cell-imaging-systems/accessories/onstage-incubator.html">Invitrogen EVOS Onstage Incubator</a></b> is an environmental chamber that enables precise control of temperature, humidity, and three gases for time-lapse imaging of live cells under both physiological and nonphysiological conditions.</p></div></div></div><div class="parsys_column cq-colctrl-lt3-c2"><div container-id="cq-colctrl-lt3" comp-id="C1"><div class="image parbase section"><div id="cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-53ca6743-86fd-4cc8-a20f-7905fcf841bftabwell_60c9uiparimage_408a"><picture><source 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alt='CellInsight CX7 LZR High-Content Screening Platform with HCA Onstage Incubator' title='CellInsight CX7 LZR High-Content Screening Platform with HCA Onstage Incubator'></picture><style> #cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-53ca6743-86fd-4cc8-a20f-7905fcf841bftabwell_60c9uiparimage_408a img{ width:100%; max-width: 230px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-53ca6743-86fd-4cc8-a20f-7905fcf841bftabwell_60c9uiparimage_408a .image-contain" + "," + "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-53ca6743-86fd-4cc8-a20f-7905fcf841bftabwell_60c9uiparimage_408a .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div><div container-id="cq-colctrl-lt3" comp-id="C2"><div class="heading section"><h4><a href="/sg/en/home/life-science/cell-analysis/cellular-imaging/hcs-hca/platforms/cx7-lzr.html">CellInsight CX7 LZR High-Content Screening Platform with HCA Onstage Incubator</a></h4></div></div><div container-id="cq-colctrl-lt3" comp-id="C3"><div class="text parbase section"><p>The <b><a href="/sg/en/home/life-science/cell-analysis/cellular-imaging/hcs-hca/accessories.html">Invitrogen HCA Onstage Incubator</a></b> for <b><a href="/sg/en/home/life-science/cell-analysis/cellular-imaging/hcs-hca/accessories.html">Thermo Scientific CellInsight HCA platforms</a></b> allows precise control of temperature, humidity, and CO<sub>2</sub> levels so that you may observe and measure biological activity and changes over time. Data gathered from longer-term imaging studies are the basis of quantitative analysis studies, especially when combined with <b><a href="/sg/en/home/life-science/cell-analysis/cellular-imaging/hcs-hca/features.html">Thermo Scientific HCS Studio Software</a></b> for increased statistical power.</p></div></div></div></div><div style="clear:both"></div></div></div></div></div></div><div class="text parbase section"><div class="table-component-wrapper"><table style="background-color: #fcf8e3;" cellspacing="0" cellpadding="10" border="0" id="table-1944508983"><tbody><tr><td><h3>Tips</h3><ul><li>For short-term imaging experiments, use a large volume of imaging medium to prevent changes in osmolarity and oxygen resulting from evaporation of the medium.</li><li>To focus on a sample, start with a low magnification. This will minimize the time the sample is exposed to light.</li><li>Avoid using autofocus for every image taken during time-lapse imaging. Autofocus can increase the amount of light energy hitting the sample by as much as 10 times.</li><li>For longer time-course imaging or imaging of sensitive cells, an onstage incubator (OSI) may be added to the imaging equipment to allow precious control of temperature, humidity, and CO<sub>2</sub> levels.</li></ul></td></tr></tbody></table></div></div></div><div class="end"></div></div></div></div></div><script> var navWorkFlow = $('.nav-workflow'), tallestTab = 0; navWorkFlow.find('li').each(function(){ if ($(this).height() > tallestTab) { tallestTab = $(this).height(); } }); navWorkFlow.find('a').css('height',tallestTab); </script><div class="workflow-accordion accordion" id="accordion-53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0"><div class="accordion-group"><div class="accordion-heading active"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0" href="#collapse1"><span class="caret"></span><strong> Step 1 : Plan </strong></a></div><div id="collapse1" class="accordion-body collapse in "><div class="accordion-inner container-0d3730df-2531-4439-a5db-7f16cbd279d2"><div class="container-0d3730df-2531-4439-a5db-7f16cbd279d2 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h2> Step 1: Plan </h2></div><div class="heading section"><h3> Design your experiment with careful consideration of the tools and resources needed for each step. </h3></div><div class="text parbase section"><h4>Advantages</h4><ul><li>Observe dynamic cellular processes as they happen</li><li>Study and image several processes and functions simultaneously using multiplexed assays</li><li>Study cellular structures in their native environment, resulting in more realistic results closer to in vivo scenarios</li><li>Track cellular biomolecules and structures over time</li><li>Observe interactions between cells</li><li>Cellular enzymes and other cytosolic biomolecules remain in the cell</li></ul></div><div class="text parbase section"><h4>Considerations</h4><ul><li>Must have a specific way to label your target with minimal toxicity – whether it is a molecule, a cellular function, or a cellular state</li><li>Living cells are generally not permeable to large detection molecules such as antibodies</li><li>Moving objects can be more difficult to keep in focus</li><li>Certain techniques can be harmful to living cells</li><li>Cells must be kept in their natural physiological state</li></ul></div><div class="well-cmp well-default well-blue-light section"><div class="well well-blue-light well-default"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><h3> Resource highlights </h3></div><div class="text parbase section"><ul class="unstyled"><li><a href="/sg/en/home/life-science/cell-analysis/cell-analysis-learning-center/cell-imaging-information.html">Learning Center: cell imaging</a></li><li><a href="/sg/en/home/life-science/cell-analysis/cell-analysis-learning-center/molecular-probes-school-of-fluorescence/imaging-basics.html">Molecular Probes School of Fluorescence: imaging basics</a></li><li><a href="/order/stain-it/#!/">Cell staining tool</a></li><li><a href="/order/fluorescence-spectraviewer#!/">Fluorescence SpectraViewer</a></li><li><a href="/sg/en/home/technical-resources/technical-reference-library/cell-analysis-support-center/cell-imaging-support.html">Cell imaging support</a></li><li><a href="/sg/en/home/technical-resources/contact-us.html">Contact us</a></li></ul></div></div></div></div></div></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0" href="#collapse2"><span class="caret"></span><strong> Step 2 : Culture </strong></a></div><div id="collapse2" class="accordion-body collapse "><div class="accordion-inner container-a8c58e24-0696-4024-b994-eec4b59254ef"><div class="container-a8c58e24-0696-4024-b994-eec4b59254ef tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h2> Step 2: Culture </h2></div><div class="heading section"><h3> Maintain or grow your cells in optimum conditions. </h3></div><div class="text parbase section"><p>Keeping cells alive and healthy during various experimental manipulations, detection, and imaging is no small task. The choice of medium is particularly important for time-lapse imaging and experiments where cells are exposed to ambient conditions for longer periods. For reliable results with live cells, it is essential that the cells be healthy and kept in an environment as close as possible to physiological temperature, pH, oxygen level, and other conditions.</p><p><a class="cta-link" href="/sg/en/home/references/gibco-cell-culture-basics.html">Learn more about cell culture</a></p></div><div class="well-cmp well-default well-blue-light section"><div class="well well-blue-light well-default"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><h3> Product highlights </h3></div><div class="text parbase section"><p>These media and wash buffers are created specifically for live-cell imaging and detection. Employing them in your experiments can help you improve image clarity, reduce background fluorescence, and optimize cell viability.</p><ul><li><b><a href="/sg/en/home/life-science/cell-culture/cell-culture-plastics.html">Thermo Scientific Nunc cell culture vessels with Nunclon Delta surface treatment</a></b> have been validated with Gibco media to confirm consistent cell growth across multiple cell lines. It’s a proven combination for happy cells and happy scientists.</li><li><b><a href="/sg/en/home/life-science/cell-culture/organoids-spheroids-3d-cell-culture/extracellular-matrices-ecm.html">Gibco extracellular matrices, scaffolds, and proteins</a></b> provide in vivo–like morphology and physiologically relevant environments for more realistic cell biology and better intercellular interactions.</li><li><b><a href="/sg/en/home/life-science/cell-analysis/cell-analysis-instruments/automated-cell-counters.html">Invitrogen Countess 3 FL Automated Cell Counter</a></b> is an affordable and automated tool for checking the health of your cells quickly and objectively. It has reusable or disposable slide options.</li></ul></div></div></div></div></div></div><div class="text parbase section"><div class="table-component-wrapper"><table style="background-color: #fcf8e3;" cellspacing="0" cellpadding="10" border="0" id="table--1960176123"><tbody><tr><td><h3>Tips</h3><p>You can improve image clarity, reduce background fluorescence, and optimize cell viability by using media and wash buffers created specifically for live-cell imaging and detection. <a href="#prodselect" class="cta-link">See product selection guide</a></p></td></tr></tbody></table></div></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0" href="#collapse3"><span class="caret"></span><strong> Step 3 : Label </strong></a></div><div id="collapse3" class="accordion-body collapse "><div class="accordion-inner container-b14a8763-2978-4940-9089-7618b2c4f1f9"><div class="container-b14a8763-2978-4940-9089-7618b2c4f1f9 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h2> Step 3: Label </h2></div><div class="heading section"><h3> Target cell structures, cell functions, and proteins of interest with selective dyes and stains. </h3></div><div class="text parbase section"><p>The appropriate fluorophore (targeted fluorescent protein or small membrane-permeant reagent) should be used to monitor your target cellular structure or process. Additional fluorophores can be used to monitor multiple cellular structures and processes, but the excitation and emission spectra should be checked using the <a href="/order/fluorescence-spectraviewer#!/">Fluorescence SpectraViewer</a> or <a href="/order/stain-it/#!/">Stain-iT Cell Staining Simulator</a> to ensure minimal spectra overlap. It is critical to avoid using too much fluorescent label because excessive fluorescent labeling can result in:</p><ul><li>Nonspecific staining with increased background signals</li><li>Physiological artifacts and structural perturbations</li><li>Cytotoxicity</li><li>Spectral overlap</li></ul><h4>Note that:</h4><ul><li>Live-cell structure reagents—help identify cellular components</li><li>Live-cell function reagents—help identify cellular functions and processes</li></ul></div><div class="well-cmp well-default well-blue-light section"><div class="well well-blue-light well-default"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><h3> Product highlights </h3></div><div class="text parbase section"><ul><li><b><a href="/sg/en/home/life-science/cell-analysis/cell-structure/celllight-ready-to-use-fluorescent-protein-based-reagents.html">Invitrogen CellLight reagents</a></b> have proven to be the easiest to use for labeling specific structures in live cells. Targeted fluorescent proteins are introduced using the Invitrogen BacMam transduction system; no molecular biology techniques are required. Simply add the reagent to your cells, incubate overnight, and you’re ready to image in the morning.</li><li><b><a href="/sg/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology.html">Invitrogen CellTracker reagents</a></b> are a diverse reagent class used for labeling mammalian cells to view changes in morphology or location. These nontoxic fluorescent dyes are designed to freely pass through cell membranes into cells, where they are transformed into cell-impermeant reaction products. Incubating cells with a CellTracker reagent for 30 minutes will provide at least 72 hours of fluorescent signal (typically three to six generations).</li><li><b><a href="/sg/en/home/brands/molecular-probes/key-molecular-probes-products/phrodo-indicators.html">Invitrogen pHrodo indicators</a></b> are fluorogenic dyes that dramatically increase in fluorescence as the pH of their surroundings becomes more acidic. When conjugated to dextrans, proteins, or other particles, pHrodo dyes can be used as highly specific sensors of endocytic and phagocytic internalization and lysosomal sequestration in live cells, offering a superior alternative to conjugates of other fluorescent dyes such as fluorescein and tetramethylrhodamine.</li></ul></div></div></div></div></div></div><div class="text parbase section"><div class="table-component-wrapper"><table style="background-color: #fcf8e3;" cellspacing="0" cellpadding="10" border="0" id="table--64949364"><tbody><tr><td><h3>Tips</h3><ul><li>Consider using a longer wavelength fluorescent reagent if extended light exposure is required. This will require lower excitation power, which can correlate to lower phototoxicity and healthier cells.</li><li>Staining must be optimized for the particular assay readout, spectral compatibility, and signal-to-background ratio.</li><li>Removing the labeling solution and rinsing with fresh medium will reduce background fluorescence.</li></ul></td></tr></tbody></table></div></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0" href="#collapse4"><span class="caret"></span><strong> Step 4 : Optimize </strong></a></div><div id="collapse4" class="accordion-body collapse "><div class="accordion-inner container-755e4acd-6798-4e66-8837-5047e5c51b9f"><div class="container-755e4acd-6798-4e66-8837-5047e5c51b9f tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h2> Step 4: Optimize </h2></div><div class="heading section"><h3> Minimize background and maintain photostability of fluorescence signals. </h3></div><div class="text parbase section"><p>Signal-to-background ratio can be optimized by using reagents that reduce extracellular fluorescence and increase fluorophore photostability. It is important to image in media that have been specifically designed for maintaining cell health while reducing or eliminating background fluorescence in live-cell imaging experiments (see <b>Table 1</b>). The addition of a background suppressor compatible with live cells can also help reduce extracellular background fluorescence and eliminate the need for a wash step. <a href="/order/catalog/product/P36975">ProLong Live Antifade Reagent</a> can be applied to samples to reduce photobleaching of fluorophores, preventing signal loss with multiple or long exposures.</p></div><div class="text parbase section"><p><b>Table 1. Imaging media comparison.</b></p><div class="table-component-wrapper"><table class="rte-table" id="table--2107675877"><tbody><tr><th>Reagent</th><th>Cell washing</th><th>Short-term imaging</th><th>Imaging up to 4 hours</th><th>Long-term imaging</th></tr><tr><td><b><a href="/order/catalog/product/10010023">Gibco PBS, pH 7.4</a></b></td><td style="text-align: center;"><img src="/content/dam/LifeTech/migration/images/products-services/cvc.par.97777.image.24.24.1..gif"></td><td style="text-align: center;"><img src="/content/dam/LifeTech/migration/images/products-services/cvc.par.97777.image.24.24.1..gif"></td><td> </td><td> </td></tr><tr><td><b><a href="/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cell-culture-media/dmem.html">Gibco FluoroBrite DMEM</a></b></td><td style="text-align: center;"><img src="/content/dam/LifeTech/migration/images/products-services/cvc.par.97777.image.24.24.1..gif"></td><td style="text-align: center;"><img src="/content/dam/LifeTech/migration/images/products-services/cvc.par.97777.image.24.24.1..gif"></td><td style="text-align: center;"><img src="/content/dam/LifeTech/migration/images/products-services/cvc.par.97777.image.24.24.1..gif"></td><td style="text-align: center;"><img src="/content/dam/LifeTech/migration/images/products-services/cvc.par.97777.image.24.24.1..gif"></td></tr><tr><td><a href="/order/catalog/product/A59688DJ?SID=srch-srp-A59688DJ"><b>Invitrogen Live Cell Imaging Solution</b></a></td><td style="text-align: center;"><img src="/content/dam/LifeTech/migration/images/products-services/cvc.par.97777.image.24.24.1..gif"></td><td style="text-align: center;"><img src="/content/dam/LifeTech/migration/images/products-services/cvc.par.97777.image.24.24.1..gif"></td><td style="text-align: center;"><img src="/content/dam/LifeTech/migration/images/products-services/cvc.par.97777.image.24.24.1..gif"></td><td> </td></tr></tbody></table></div></div><div class="well-cmp well-default well-blue-light section"><div class="well well-blue-light well-default"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><h3> Product highlights </h3></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="text parbase section"><ul><li><a href="/order/catalog/product/B10512">Invitrogen BackDrop Background Suppressor</a> is used when observing high background signal or weak fluorescence in the blue, green, or red channels.</li><li><a href="/order/catalog/product/10010023">Gibco PBS, pH 7.4</a> is ideal for cell washing and short-term imaging where prolonged incubation is not required.</li><li><a href="/order/catalog/product/A59688DJ?SID=srch-srp-A59688DJ">Invitrogen Live Cell Imaging Solution</a> is an optically clear solution used for imaging, dye loading, and wash steps. It helps keep cells healthy for up to 4 hours.</li></ul></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="image parbase section"><div id="cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-755e4acd-6798-4e66-8837-5047e5c51b9ftabwell_1769uiparimage_7f14"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_7f14/foregroundimg.img.full.high.jpg/1738165969838.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_7f14/foregroundimg.img.full.high.jpg/1738165969838.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_7f14/foregroundimg.img.320.high.jpg/1738165969838.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_7f14/foregroundimg.img.480.high.jpg/1738165969838.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_7f14/foregroundimg.img.320.medium.jpg/1738165969838.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_7f14/foregroundimg.img.620.high.jpg/1738165969838.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_7f14/foregroundimg.img.320.low.jpg/1738165969838.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_7f14/foregroundimg.img.480.medium.jpg/1738165969838.jpg" alt='Invitrogen BackDrop Background Suppressor imaging results' title='Invitrogen BackDrop Background Suppressor imaging results'></picture><style> #cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-755e4acd-6798-4e66-8837-5047e5c51b9ftabwell_1769uiparimage_7f14 img{ width:100%; max-width: 450px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-755e4acd-6798-4e66-8837-5047e5c51b9ftabwell_1769uiparimage_7f14 .image-contain" + "," + "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-755e4acd-6798-4e66-8837-5047e5c51b9ftabwell_1769uiparimage_7f14 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script><div class="caption"><small><p><b>Live HeLa cells labeled with <a href="/order/catalog/product/T34075">Tubulin Tracker Green dye</a> and <a href="/order/catalog/product/T34076">Tubulin Tracker Deep Red dye</a>.</b> Both labels show high off-cell background when the probe is left in the staining solution (left). Addition of <a href="/order/catalog/product/B10512">BackDrop Background Suppressor</a> greatly reduces extracellular background while leaving intracellular labeling unaffected (right), thus enabling a no-wash protocol for high-contrast imaging of tubulin in live cells.</p></small></div></div></div></div></div></div><div style="clear:both"></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-755e4acd-6798-4e66-8837-5047e5c51b9ftabwell_1769uiparimage_b4a1"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_b4a1/foregroundimg.img.full.high.jpg/1738165969885.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_b4a1/foregroundimg.img.full.high.jpg/1738165969885.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_b4a1/foregroundimg.img.320.high.jpg/1738165969885.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_b4a1/foregroundimg.img.480.high.jpg/1738165969885.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_b4a1/foregroundimg.img.320.medium.jpg/1738165969885.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_b4a1/foregroundimg.img.620.high.jpg/1738165969885.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_b4a1/foregroundimg.img.320.low.jpg/1738165969885.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_b4a1/foregroundimg.img.480.medium.jpg/1738165969885.jpg" alt='Invitrogen ProLong Live Reagent confocal imaging results' title='Invitrogen ProLong Live Reagent confocal imaging results'></picture><style> #cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-755e4acd-6798-4e66-8837-5047e5c51b9ftabwell_1769uiparimage_b4a1 img{ width:100%; max-width: 450px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-755e4acd-6798-4e66-8837-5047e5c51b9ftabwell_1769uiparimage_b4a1 .image-contain" + "," + "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-755e4acd-6798-4e66-8837-5047e5c51b9ftabwell_1769uiparimage_b4a1 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script><div class="caption"><small><p class="muted"><b>The overall signal protection offered by <a href="/order/catalog/product/P36974">ProLong Live reagent</a></b>compared to untreated samples is calculated based on the scan number where treated and untreated samples reach the EC50 value. The addition of ProLong Live reagent permitted 100% more captures with <a href="/order/catalog/product/C10601">Invitrogen CellLight Mitochondria-RFP reagent</a>.</p></small></div></div></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="image parbase section"><div id="cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-755e4acd-6798-4e66-8837-5047e5c51b9ftabwell_1769uiparimage_aee7"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_aee7/foregroundimg.img.full.high.jpg/1738165969926.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_aee7/foregroundimg.img.full.high.jpg/1738165969926.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_aee7/foregroundimg.img.320.high.jpg/1738165969926.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_aee7/foregroundimg.img.480.high.jpg/1738165969926.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_aee7/foregroundimg.img.320.medium.jpg/1738165969926.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_aee7/foregroundimg.img.620.high.jpg/1738165969926.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_aee7/foregroundimg.img.320.low.jpg/1738165969926.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-755e4acd-6798-4e66-8837-5047e5c51b9f/tab/well_1769/uipar/image_aee7/foregroundimg.img.480.medium.jpg/1738165969926.jpg" alt='Invitrogen ProLong Live Reagent fluorescence imaging' title='Invitrogen ProLong Live Reagent fluorescence imaging'></picture><style> #cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-755e4acd-6798-4e66-8837-5047e5c51b9ftabwell_1769uiparimage_aee7 img{ width:100%; max-width: 450px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-755e4acd-6798-4e66-8837-5047e5c51b9ftabwell_1769uiparimage_aee7 .image-contain" + "," + "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-755e4acd-6798-4e66-8837-5047e5c51b9ftabwell_1769uiparimage_aee7 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script><div class="caption"><small><p class="muted"><b>After 120 exposures using a standard time-lapse imaging protocol,</b> samples treated with <a href="/order/catalog/product/P36974">ProLong Live reagent</a> are >20% brighter than untreated cells, enabling more data collection time.</p></small></div></div></div></div></div></div><div style="clear:both"></div></div></div></div></div></div><div class="text parbase section"><div class="table-component-wrapper"><table style="background-color: #fcf8e3;" cellspacing="0" cellpadding="10" border="0" id="table--1347696705"><tbody><tr><td><h3>Tips</h3><ul><li>If no further culture is planned, a background suppressor can be used to optimize the signal by reducing the haze and increasing the contrast.</li><li>The use of an antifade reagent has been shown to increase fluorophore photostability and decrease the effect of phototoxicity in a variety of sample types.</li></ul></td></tr></tbody></table></div></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0" href="#collapse5"><span class="caret"></span><strong> Step 5 : Image </strong></a></div><div id="collapse5" class="accordion-body collapse "><div class="accordion-inner container-53ca6743-86fd-4cc8-a20f-7905fcf841bf"><div class="container-53ca6743-86fd-4cc8-a20f-7905fcf841bf tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h2> Step 5: Image </h2></div><div class="heading section"><h3> Live-cell imaging of dynamic processes requires active observation over time </h3></div><div class="text parbase section"><h4>Illumination and detection</h4><p>To minimize phototoxicity, choose imaging systems that give you the greatest control of light sources. Try to minimize light intensity, exposure time, wavelength range, and amount of excitation energy for illuminating your cells while still generating a good signal with low background. Use the illumination that gives you the highest signal with the lowest level of fluorophore excitation. In some cases (particularly when you wish to image over a long period of time), it is advisable to sacrifice resolution by using shorter exposure times or lower magnification in exchange for healthier cells.</p><p>Live-cell imaging over longer periods of time can be challenging because the target may move out of focus during the course of the experiment. Many microscopes have autofocusing features that can help keep your target in focus longer and reduce focal drift. Additionally, maintaining cells at a constant temperature and keeping the volume of solution in the vessel constant will help with focal drift.</p><h4>Environmental control</h4><p>Many cells cannot tolerate deviations from their optimal temperature, osmolarity, pH, and humidity. Requirements vary depending on what experimental question you are asking. For example, experiments investigating cell growth and division may have a different set of requirements than experiments involving receptor activation and calcium accumulation. Some robust immortalized cell lines will tolerate being imaged or monitored for short periods of time without any environmental control. Conversely, for long-term imaging and detection studies, good results with both immortalized cells and primary cells typically require tightly controlled environmental parameters.</p></div><div class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div class="image parbase section"><div id="cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-53ca6743-86fd-4cc8-a20f-7905fcf841bftabimage_12db"><a class="image-enlarge-link" data-lightbox="image_12db" href="/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/_jcr_content/MainParsys/tabworkflowcontainer_0/container-53ca6743-86fd-4cc8-a20f-7905fcf841bf/tab/image_12db/backgroundimg.img.jpg/1738165970099.jpg"><picture><source 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srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-53ca6743-86fd-4cc8-a20f-7905fcf841bf/tab/image_12db/foregroundimg.img.full.high.jpg/1738165970077.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-53ca6743-86fd-4cc8-a20f-7905fcf841bf/tab/image_12db/foregroundimg.img.320.high.jpg/1738165970077.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-53ca6743-86fd-4cc8-a20f-7905fcf841bf/tab/image_12db/foregroundimg.img.480.high.jpg/1738165970077.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-53ca6743-86fd-4cc8-a20f-7905fcf841bf/tab/image_12db/foregroundimg.img.320.medium.jpg/1738165970077.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-53ca6743-86fd-4cc8-a20f-7905fcf841bf/tab/image_12db/foregroundimg.img.620.high.jpg/1738165970077.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-53ca6743-86fd-4cc8-a20f-7905fcf841bf/tab/image_12db/foregroundimg.img.320.low.jpg/1738165970077.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-53ca6743-86fd-4cc8-a20f-7905fcf841bf/tab/image_12db/foregroundimg.img.480.medium.jpg/1738165970077.jpg" alt='HDFn cells loaded with CellTracker Deep Red Dye' title='HDFn cells loaded with CellTracker Deep Red Dye'></picture><style> #cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-53ca6743-86fd-4cc8-a20f-7905fcf841bftabimage_12db img{ width:100%; max-width: 1050px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-53ca6743-86fd-4cc8-a20f-7905fcf841bftabimage_12db .image-contain" + "," + "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-53ca6743-86fd-4cc8-a20f-7905fcf841bftabimage_12db .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a><div class="caption"><small><span class=" icon-zoom-in"> </span>Click image to enlarge</small></div></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div class="text parbase section"><p><small class="muted"><b>A scratch wound in a culture of HDFn cells loaded with <a href="/order/catalog/product/C34565">Invitrogen CellTracker Deep Red Dye</a>.</b> (<b>A</b>) The illuminated area was subjected to repeated illumination for 10 hours. Cells in this area show signs of phototoxicity (a loss of viability as cells were not able to grow into the wound). (<b>B</b>) Cells in the non-illuminated area show viable cell growth into the wound.</small></p></div></div></div><div style="clear:both"></div><div class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div class="image parbase section"><div id="cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-53ca6743-86fd-4cc8-a20f-7905fcf841bftabimage_fd1f"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-53ca6743-86fd-4cc8-a20f-7905fcf841bf/tab/image_fd1f/foregroundimg.img.full.high.jpg/1738165970134.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-53ca6743-86fd-4cc8-a20f-7905fcf841bf/tab/image_fd1f/foregroundimg.img.full.high.jpg/1738165970134.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-53ca6743-86fd-4cc8-a20f-7905fcf841bf/tab/image_fd1f/foregroundimg.img.320.high.jpg/1738165970134.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-53ca6743-86fd-4cc8-a20f-7905fcf841bf/tab/image_fd1f/foregroundimg.img.480.high.jpg/1738165970134.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-53ca6743-86fd-4cc8-a20f-7905fcf841bf/tab/image_fd1f/foregroundimg.img.320.medium.jpg/1738165970134.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-53ca6743-86fd-4cc8-a20f-7905fcf841bf/tab/image_fd1f/foregroundimg.img.620.high.jpg/1738165970134.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-53ca6743-86fd-4cc8-a20f-7905fcf841bf/tab/image_fd1f/foregroundimg.img.320.low.jpg/1738165970134.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-53ca6743-86fd-4cc8-a20f-7905fcf841bf/tab/image_fd1f/foregroundimg.img.480.medium.jpg/1738165970134.jpg" alt='Catastrophic blebbing of the cell membrane' title='Catastrophic blebbing of the cell membrane'></picture><style> #cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-53ca6743-86fd-4cc8-a20f-7905fcf841bftabimage_fd1f img{ width:100%; max-width: 400px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-53ca6743-86fd-4cc8-a20f-7905fcf841bftabimage_fd1f .image-contain" + "," + "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-53ca6743-86fd-4cc8-a20f-7905fcf841bftabimage_fd1f .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div class="text parbase section"><p><small class="muted"><b>The top cell shows catastrophic blebbing of the cell membrane caused by excessive light exposure.</b> Blebbing is a term used to describe membrane perturbation caused by toxicity. By contrast, the bottom cell remains relatively healthy and is not displaying aberrant morphology.</small></p></div></div></div><div style="clear:both"></div><div class="well-cmp well-default well-blue-light section"><div class="well well-blue-light well-default"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><h3> Product highlights </h3></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt3"><div class="parsys_column cq-colctrl-lt3-c0"><div container-id="cq-colctrl-lt3" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-53ca6743-86fd-4cc8-a20f-7905fcf841bftabwell_60c9uiparimage_af9a"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-53ca6743-86fd-4cc8-a20f-7905fcf841bf/tab/well_60c9/uipar/image_af9a/foregroundimg.img.full.high.jpg/1738165970278.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-53ca6743-86fd-4cc8-a20f-7905fcf841bf/tab/well_60c9/uipar/image_af9a/foregroundimg.img.full.high.jpg/1738165970278.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-53ca6743-86fd-4cc8-a20f-7905fcf841bf/tab/well_60c9/uipar/image_af9a/foregroundimg.img.320.high.jpg/1738165970278.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-53ca6743-86fd-4cc8-a20f-7905fcf841bf/tab/well_60c9/uipar/image_af9a/foregroundimg.img.480.high.jpg/1738165970278.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-53ca6743-86fd-4cc8-a20f-7905fcf841bf/tab/well_60c9/uipar/image_af9a/foregroundimg.img.320.medium.jpg/1738165970278.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-53ca6743-86fd-4cc8-a20f-7905fcf841bf/tab/well_60c9/uipar/image_af9a/foregroundimg.img.620.high.jpg/1738165970278.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-53ca6743-86fd-4cc8-a20f-7905fcf841bf/tab/well_60c9/uipar/image_af9a/foregroundimg.img.320.low.jpg/1738165970278.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-53ca6743-86fd-4cc8-a20f-7905fcf841bf/tab/well_60c9/uipar/image_af9a/foregroundimg.img.480.medium.jpg/1738165970278.jpg" alt='Countess II FL Automated Cell Counter' title='Countess II FL Automated Cell Counter'></picture><style> #cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-53ca6743-86fd-4cc8-a20f-7905fcf841bftabwell_60c9uiparimage_af9a img{ width:100%; max-width: 230px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-53ca6743-86fd-4cc8-a20f-7905fcf841bftabwell_60c9uiparimage_af9a .image-contain" + "," + "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-53ca6743-86fd-4cc8-a20f-7905fcf841bftabwell_60c9uiparimage_af9a .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div><div container-id="cq-colctrl-lt3" comp-id="A2"><div class="heading section"><h4><a href="/sg/en/home/life-science/cell-analysis/cell-analysis-instruments/automated-cell-counters.html">Countess 3 FL Automated Cell Counter</a></h4></div></div><div container-id="cq-colctrl-lt3" comp-id="A3"><div class="text parbase section"><p>To avoid the pitfall of proceeding to the next step in your experiment with unhealthy cells, a quick check for cell health can be done on the <b><a href="/sg/en/home/life-science/cell-analysis/cell-analysis-instruments/automated-cell-counters.html">Countess 3 FL Automated Cell Counter</a></b> when used in conjunction with a variety of fluorescent reagents to detect cell viability, apoptosis, cytotoxicity, and transfection efficiency. The reusable slide option reduces consumption cost.</p></div></div></div><div class="parsys_column cq-colctrl-lt3-c1"><div container-id="cq-colctrl-lt3" comp-id="B1"><div class="image parbase section"><div id="cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-53ca6743-86fd-4cc8-a20f-7905fcf841bftabwell_60c9uiparimage_67b7"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/tabworkflowcontainer_0/container-53ca6743-86fd-4cc8-a20f-7905fcf841bf/tab/well_60c9/uipar/image_67b7/foregroundimg.img.full.high.jpg/1738165970335.jpg' media="(min-width: 1px)"><img 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alt='Invitrogen EVOS M5000 Imaging System with EVOS Onstage Incubator' title='Invitrogen EVOS M5000 Imaging System with EVOS Onstage Incubator'></picture><style> #cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-53ca6743-86fd-4cc8-a20f-7905fcf841bftabwell_60c9uiparimage_67b7 img{ width:100%; max-width: 230px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-53ca6743-86fd-4cc8-a20f-7905fcf841bftabwell_60c9uiparimage_67b7 .image-contain" + "," + "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-53ca6743-86fd-4cc8-a20f-7905fcf841bftabwell_60c9uiparimage_67b7 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div><div container-id="cq-colctrl-lt3" comp-id="B2"><div class="heading section"><h4><a href="/sg/en/home/life-science/cell-analysis/cellular-imaging/evos-cell-imaging-systems/accessories/onstage-incubator.html">Invitrogen EVOS M5000 Imaging System with EVOS Onstage Incubator</a></h4></div></div><div container-id="cq-colctrl-lt3" comp-id="B3"><div class="text parbase section"><p>Designed specifically for <b><a href="/sg/en/home/life-science/cell-analysis/cellular-imaging/evos-cell-imaging-systems.html">Invitrogen EVOS imaging systems</a></b>, the <b><a href="/sg/en/home/life-science/cell-analysis/cellular-imaging/evos-cell-imaging-systems/accessories/onstage-incubator.html">Invitrogen EVOS Onstage Incubator</a></b> is an environmental chamber that enables precise control of temperature, humidity, and three gases for time-lapse imaging of live cells under both physiological and nonphysiological conditions.</p></div></div></div><div class="parsys_column cq-colctrl-lt3-c2"><div container-id="cq-colctrl-lt3" comp-id="C1"><div class="image parbase section"><div id="cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-53ca6743-86fd-4cc8-a20f-7905fcf841bftabwell_60c9uiparimage_408a"><picture><source 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alt='CellInsight CX7 LZR High-Content Screening Platform with HCA Onstage Incubator' title='CellInsight CX7 LZR High-Content Screening Platform with HCA Onstage Incubator'></picture><style> #cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-53ca6743-86fd-4cc8-a20f-7905fcf841bftabwell_60c9uiparimage_408a img{ width:100%; max-width: 230px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-53ca6743-86fd-4cc8-a20f-7905fcf841bftabwell_60c9uiparimage_408a .image-contain" + "," + "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsystabworkflowcontainer_0container-53ca6743-86fd-4cc8-a20f-7905fcf841bftabwell_60c9uiparimage_408a .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div><div container-id="cq-colctrl-lt3" comp-id="C2"><div class="heading section"><h4><a href="/sg/en/home/life-science/cell-analysis/cellular-imaging/hcs-hca/platforms/cx7-lzr.html">CellInsight CX7 LZR High-Content Screening Platform with HCA Onstage Incubator</a></h4></div></div><div container-id="cq-colctrl-lt3" comp-id="C3"><div class="text parbase section"><p>The <b><a href="/sg/en/home/life-science/cell-analysis/cellular-imaging/hcs-hca/accessories.html">Invitrogen HCA Onstage Incubator</a></b> for <b><a href="/sg/en/home/life-science/cell-analysis/cellular-imaging/hcs-hca/accessories.html">Thermo Scientific CellInsight HCA platforms</a></b> allows precise control of temperature, humidity, and CO<sub>2</sub> levels so that you may observe and measure biological activity and changes over time. Data gathered from longer-term imaging studies are the basis of quantitative analysis studies, especially when combined with <b><a href="/sg/en/home/life-science/cell-analysis/cellular-imaging/hcs-hca/features.html">Thermo Scientific HCS Studio Software</a></b> for increased statistical power.</p></div></div></div></div><div style="clear:both"></div></div></div></div></div></div><div class="text parbase section"><div class="table-component-wrapper"><table style="background-color: #fcf8e3;" cellspacing="0" cellpadding="10" border="0" id="table--161384805"><tbody><tr><td><h3>Tips</h3><ul><li>For short-term imaging experiments, use a large volume of imaging medium to prevent changes in osmolarity and oxygen resulting from evaporation of the medium.</li><li>To focus on a sample, start with a low magnification. This will minimize the time the sample is exposed to light.</li><li>Avoid using autofocus for every image taken during time-lapse imaging. Autofocus can increase the amount of light energy hitting the sample by as much as 10 times.</li><li>For longer time-course imaging or imaging of sensitive cells, an onstage incubator (OSI) may be added to the imaging equipment to allow precious control of temperature, humidity, and CO<sub>2</sub> levels.</li></ul></td></tr></tbody></table></div></div></div><div class="end"></div></div></div></div></div></div></div><div class="divider section"><hr /></div><div class="well-cmp section"><div class="well "><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><h2 id="free-webinar"> Featured webinar </h2></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt7"><div class="parsys_column cq-colctrl-lt7-c0"><div container-id="cq-colctrl-lt7" comp-id="A1"><div class="brightcoveplayer section"><div data-video="d528b96efd00"><script type="text/javascript"> var loadEME = true; function checkVideojsLoaded(callback, schemaVideoId, maxIterations) { let iterations = 0; return new Promise((resolve, reject) => { function iterate() { if(loadEME && typeof videojs != "undefined"){ videojs.registerPlugin('eme', function() {}); loadEME = false; } // Perform your value check here if (typeof videojs!="undefined" && videojs.getPlayer(schemaVideoId)) { resolve(); // Resolve the promise if the value is true } else if (iterations <= (maxIterations*1e3)) { iterations++; setTimeout(iterate, 500); // Wait for 0.5 second and iterate again } else { reject('Maximum iteration time exceeded.'); // Reject the promise if max iterations reached } } iterate(); // Start the iteration }) .then(() => { callback(); // Call the provided callback function when the value is true }) .catch((error) => { console.error('Error:', error); }); } checkVideojsLoaded(function(){ videojs.options.techOrder = ["html5"]; }, 'video-d528b96efd00', 10); </script><div id="d528b96efd00-Modal" class="modal modal-video" tabindex="-1" role="dialog" aria-labelledby="d528b96efd00-Modal-Label" aria-hidden="true" style="display: none;"><div class="modal-header"><button type="button" class="close" data-dismiss="modal" aria-hidden="true">x</button></div><div data-video-modal-body="d528b96efd00" class="modal-body"></div></div><script> $(document).ready(function(){ $('#d528b96efd00').bind('click', function () { $.ajax({url: '/content/lifetech/ipac/en-sg/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/well_6486/uipar/brightcoveplayer_a19.ajax.html?cached=false', data: { account: '3663210762001', videoPlayer: '6082967353001', playerPath: '' }, success: function(result) { $('[data-video-modal-body*=d528b96efd00]').html(result); }}); }); $('#d528b96efd00-Modal').bind('show', function () { $.ajax({url: '/content/lifetech/ipac/en-sg/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/well_6486/uipar/brightcoveplayer_a19.ajax.html?cached=false', data: { account: '3663210762001', videoPlayer: '6082967353001', playerPath: '' }, success: function(result) { $('[data-video-modal-body*=d528b96efd00]').html(result); }}); }); }); $('#d528b96efd00-Modal').bind('hide', function () { var htmlVideoId = $('[data-video-id*=6082967353001]').attr('id'); try { videojs(htmlVideoId).dispose(); } catch (e) { console.log('Unable to destroy videojs player.'); } $('[data-video-modal-body*=d528b96efd00]').html(''); }); </script></div><a id="d528b96efd00" data-target="#d528b96efd00-Modal" class="link-overlay-play" data-toggle="modal"><img id="d528b96efd00-Modal-Thumbnail" style="width: 100%; height: auto;" src="/bin/brightcove/image.jpeg?id=6082967353001&key=3663210762001"/></a><script src="//players.brightcove.net/3663210762001/08UsfMRkC_default/index.min.js"></script><script type="text/javascript"> var schemaObj = { "@context": "https://schema.org", "@type": "VideoObject", "name": "Best practices: 5 steps to live-cell imaging", "description": "Whether you are new to live-cell imaging or you are an experienced researcher, this webinar will show you how to obtain publication-quality live-cell images every time while avoiding the frustration of wasted time and resources. 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Please use a different browser to view these videos.</div>") .addClass("unsuppported-videoplayer") .insertAfter($(".brightcoveplayer, .cmp-p-videoplayer")); } </script></div></div></div><div class="parsys_column cq-colctrl-lt7-c1"><div container-id="cq-colctrl-lt7" comp-id="B1"><div class="heading section"><h3> Best practices: 5 steps to live-cell imaging </h3></div></div><div container-id="cq-colctrl-lt7" comp-id="B2"><div class="text parbase section"><p>Whether you are new to live-cell imaging or you are an experienced researcher, this webinar will show you how to obtain publication-quality live-cell images while avoiding the frustration of wasted time and resources.</p></div></div></div></div><div style="clear:both"></div></div></div></div></div></div><div class="heading section"><h2 id="images-and-videos"> Examples of live-cell images and videos </h2></div><div class="LTRawHTML section"><script> $(document).ready(function() { $('#5steps-gallery').carousel('pause'); }); </script><style> .carousel-caption { position: relative; padding: 0; background: #fff; } .carousel-caption p { color: #8a8a8a; } .carousel-inner .item img { max-width: 710px; } .carousel-indicators { right: 0; left: 0; text-align: center; } .carousel-indicators li { float: none; display: inline-block; } .carousel-indicators li:first-child { margin-left: 0; } </style><div id="5steps-gallery" class="carousel slide" data-interval="false"><ol class="carousel-indicators"><li data-target="#5steps-gallery" data-slide-to="0" class="active"></li><li data-target="#5steps-gallery" data-slide-to="1"></li><li data-target="#5steps-gallery" data-slide-to="2"></li><li data-target="#5steps-gallery" data-slide-to="3"></li><li data-target="#5steps-gallery" data-slide-to="4"></li><li data-target="#5steps-gallery" data-slide-to="5"></li><li data-target="#5steps-gallery" data-slide-to="6"></li><li data-target="#5steps-gallery" data-slide-to="7"></li><li data-target="#5steps-gallery" data-slide-to="8"></li><li data-target="#5steps-gallery" data-slide-to="9"></li><li data-target="#5steps-gallery" data-slide-to="10"></li><li data-target="#5steps-gallery" data-slide-to="11"></li><li data-target="#5steps-gallery" data-slide-to="12"></li></ol><div class="carousel-inner"><div class="active item"><img src="/content/dam/LifeTech/global/life-sciences/cellanalysis/Images/Live-HeLa-cells-labeled-Tubulin-Tracker-Deep-Red.jpg" alt=""><div class="carousel-caption"><p><small><b>Live HeLa cells labeled with <a href="/order/catalog/product/T34076">Tubulin Tracker Deep Red</a> and <a href="/order/catalog/product/R37605">NucBlue Live ReadyProbes Reagent</a></b>, enabling visualization of the microtubule cytoskeleton and nuclei in live cells, respectively. High resolution images were acquired using a confocal system.</small></p></div></div><div class="item"><img src="/content/dam/LifeTech/global/life-sciences/cellanalysis/Images/HeLa-cells-labeled-Invitrogen-Tubulin-Tracker-Green-dye.jpg" alt="HeLa cells labeled using Invitrogen Tubulin Tracker Green dye and Invitrogen MitoTracker Red CMXRos dye show superb multiplexing capability and uniformity of staining compared with overexpressed fluorescent protein fusions."><div class="carousel-caption"><p><small><b>HeLa cells labeled using <a href="/order/catalog/product/T34075">Invitrogen Tubulin Tracker Green dye</a> and <a href="/order/catalog/product/M7512">Invitrogen MitoTracker Red CMXRos dye</a> show superb multiplexing capability and uniformity of staining compared with overexpressed fluorescent protein fusions.</b> Cells were imaged in <a href="/order/catalog/product/14025134">Gibco HBSS buffer</a> containing calcium and magnesium, supplemented with <a href="/order/catalog/product/P36400">1X Probenecid solution</a>. Images were generated using an <a href="/order/catalog/product/AMAFD2000">EVOS FL Auto 2 Imaging System</a> with an <a href="/order/catalog/product/AMEP4734">Olympus 20X Super Apochromat objective</a> using <a href="/order/catalog/product/AMEP4651">GFP</a> and <a href="/order/catalog/product/AMEP4655">Texas Red</a> EVOS light cubes.</small></p></div></div><div class="item"><img src="/content/dam/LifeTech/global/life-sciences/cellanalysis/Images/HeLa-cells-labeled-NucBlue-Live-ReadyProbes-Reagent.jpg" alt="HeLa cells labeled using NucBlue Live ReadyProbes Reagent, Tubulin Tracker Green dye, and Invitrogen LysoTracker Deep Red dye show superb multiplexing capability and staining specificity."><div class="carousel-caption"><p><small><b>HeLa cells labeled using <a href="/order/catalog/product/R37605">NucBlue Live ReadyProbes Reagent</a>, <a href="/order/catalog/product/T34075">Tubulin Tracker Green dye</a>, and <a href="/order/catalog/product/L12492">Invitrogen LysoTracker Deep Red dye</a> show superb multiplexing capability and staining specificity.</b> Cells were imaged in <a href="/order/catalog/product/14025134">Gibco HBSS buffer</a> containing calcium and magnesium, supplemented with <a href="/order/catalog/product/P36400">1X Probenecid solution</a>. Images were generated using an <a href="/order/catalog/product/AMAFD2000">EVOS FL Auto 2 Imaging System</a> with an <a href="/order/catalog/product/AMEP4694">Olympus 60X Super Apochromat Oil objective</a> using <a href="/order/catalog/product/AMEP4650">DAPI</a>, <a href="/order/catalog/product/AMEP4651">GFP</a>, and <a href="/order/catalog/product/AMEP4656">Cy5</a> EVOS light cubes.</small></p></div></div><div class="item"><img src="/content/dam/LifeTech/global/life-sciences/cellanalysis/Images/U2OS-cells-labeled-Invitrogen-LysoTracker-Blue-DND22-dye.jpg" alt="U2OS cells labeled using Invitrogen LysoTracker Blue DND22 dye, Invitrogen MitoTracker Green FM dye, and Tubulin Tracker Deep Red dye show superb multiplexing capability and staining specificity."><div class="carousel-caption"><p><small><b>U2OS cells labeled using <a href="/order/catalog/product/L7525">Invitrogen LysoTracker Blue DND22 dye</a>, <a href="/order/catalog/product/M7514">Invitrogen MitoTracker Green FM dye</a>, and <a href="/order/catalog/product/T34076">Tubulin Tracker Deep Red dye</a> show superb multiplexing capability and staining specificity.</b> Cells were imaged in <a href="/order/catalog/product/14025134">Gibco HBSS buffer</a> containing calcium and magnesium, supplemented with <a href="/order/catalog/product/P36400">1X Probenecid solution</a>. Images were generated using an <a href="/order/catalog/product/AMAFD2000">EVOS FL Auto 2 Imaging System</a> with an <a href="/order/catalog/product/AMEP4694">Olympus 60X Super Apochromat Oil objective</a> using <a href="/order/catalog/product/AMEP4650">DAPI</a>, <a href="/order/catalog/product/AMEP4651">GFP</a>, and <a href="/order/catalog/product/AMEP4656">Cy5</a> EVOS light cubes.</small></p></div></div><div class="item"><img src="/content/dam/LifeTech/global/life-sciences/cellanalysis/Images/HeLa-cells-labeled-NucBlue-Live-ReadyProbes-Reagent-2.jpg" alt="HeLa cells labeled using NucBlue Live ReadyProbes Reagent, Tubulin Tracker Green, and CellLight Mitochondria-RFP show superb multiplexing capability and staining specificity."><div class="carousel-caption"><p><small><b>HeLa cells labeled using <a href="/order/catalog/product/R37605">NucBlue Live ReadyProbes Reagent</a>, <a href="/order/catalog/product/T34075">Tubulin Tracker Green</a>, and <a href="/order/catalog/product/C10601">CellLight Mitochondria-RFP</a> show superb multiplexing capability and staining specificity.</b> Cells were imaged in <a href="/order/catalog/product/14025134">Gibco HBSS buffer</a> containing calcium and magnesium, supplemented with <a href="/order/catalog/product/P36400">1X Probenecid solution</a>. Images were generated using an <a href="/order/catalog/product/AMAFD2000">EVOS FL Auto 2 Imaging System</a> with an <a href="/order/catalog/product/AMEP4694">Olympus 60X Super Apochromat Oil objective></a> using <a href="/order/catalog/product/AMEP4650">DAPI</a>, <a href="/order/catalog/product/AMEP4651">GFP</a>, and <a href="/order/catalog/product/AMEP4652">RFP</a> EVOS light cubes.</small></p></div></div><div class="item"><img src="/content/dam/LifeTech/global/life-sciences/cellanalysis/Images/HeLa-cells-labeled-NucBlue-Live-ReadyProbes-Reagent-3.jpg" alt="HeLa cells labeled using NucBlue Live ReadyProbes Reagent, ER-Tracker Green, and Tubulin Tracker Deep Red show superb multiplexing capability and uniformity of staining compared with overexpressed fluorescent protein fusions."><div class="carousel-caption"><p><small><b>HeLa cells labeled using <a href="/order/catalog/product/R37605">NucBlue Live ReadyProbes Reagent</a>, <a href="/order/catalog/product/E34251">ER-Tracker Green</a>, and <a href="/order/catalog/product/T34076">Tubulin Tracker Deep Red</a> show superb multiplexing capability and uniformity of staining compared with overexpressed fluorescent protein fusions.</b> Cells were imaged in <a href="/order/catalog/product/14025134">Gibco HBSS buffer</a> containing calcium and magnesium, supplemented with <a href="/order/catalog/product/P36400">1X Probenecid solution</a>. Images were generated using an <a href="/order/catalog/product/AMAFD2000">EVOS FL Auto 2 Imaging System</a> with an <a href="/order/catalog/product/AMEP4734">Olympus 20X Super Apochromat objective</a> using <a href="/order/catalog/product/AMEP4650">DAPI</a>, <a href="/order/catalog/product/AMEP4651">GFP</a>, and <a href="/order/catalog/product/AMEP4656">Cy5</a> EVOS light cubes.</small></p></div></div><div class="item"><img src="/content/dam/LifeTech/global/life-sciences/cellanalysis/Images/U2OS-cells-labeled-NucBlue-Live-ReadyProbes-Reagent.jpg" alt="U2OS cells labeled using NucBlue Live ReadyProbes Reagent, CellLight Actin-GFP, CellLight Mitochondria-RFP, and Tubulin Tracker Deep Red show superb multiplexing capability and staining specificity."><div class="carousel-caption"><p><small><b>U2OS cells labeled using <a href="/order/catalog/product/R37605">NucBlue Live ReadyProbes Reagent</a>, <a href="/order/catalog/product/C10582">CellLight Actin-GFP</a>, <a href="/order/catalog/product/C10601">CellLight Mitochondria-RFP</a>, and <a href="/order/catalog/product/T34076">Tubulin Tracker Deep Red</a> show superb multiplexing capability and staining specificity.</b> Cells were imaged in <a href="/order/catalog/product/14025134">Gibco HBSS buffer</a> containing calcium and magnesium, supplemented with <a href="/order/catalog/product/P36400">1X Probenecid solution</a>. Images were generated using an <a href="/order/catalog/product/AMAFD2000">EVOS FL Auto 2 Imaging System</a> with an <a href="/order/catalog/product/AMEP4694">Olympus 60X Super Apochromat Oil objective></a> using <a href="/order/catalog/product/AMEP4650">DAPI</a>, <a href="/order/catalog/product/AMEP4651">GFP</a>, <a href="/order/catalog/product/AMEP4652">RFP</a>, and <a href="/order/catalog/product/AMEP4656">Cy5</a> EVOS light cubes.</small></p></div></div><div class="item"><img src="/content/dam/LifeTech/global/life-sciences/cellanalysis/Images/HeLa-cells-transduced-CellLight-Mitochondria-GFP.jpg" alt="HeLa cells grown in MEM with 10% FBS were transduced with CellLight Mitochondria-RFP and CellLight Golgi-RFP."><div class="carousel-caption"><p><small><b>HeLa cells grown in <a href="/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cell-culture-media/mem.html">MEM</a> with 10% <a href="/sg/en/home/life-science/cell-culture/mammalian-cell-culture/fbs.html">FBS</a> were transduced with <a href="/order/catalog/product/C10600">CellLight Mitochondria-RFP</a> and <a href="/order/catalog/product/C10593">CellLight Golgi-RFP</a>. After 24 hours, nuclei were counter stained with <a href="/order/catalog/product/R37605">NucBlue Live ReadyProbes Reagent</a>.</b> Image was captured on an <a href="/order/catalog/product/AMAFD2000">EVOS FL Auto 2 Imaging System</a> using a 60X Apo objective and <a href="/order/catalog/product/AMEP4650">DAPI</a>, <a href="/order/catalog/product/AMEP4651">GFP</a> and <a href="/order/catalog/product/AMEP4652">RFP</a> LED light cubes.</small></p></div></div><div class="item"><img src="/content/dam/LifeTech/global/life-sciences/cellanalysis/Images/four-plex-image-COS7-live-cell.png" alt="Four-plex image of COS7 live cell."><div class="carousel-caption"><p><small><b>Four-plex image of COS7 live cell.</b> Tubulin is labeled with <a href="/order/catalog/product/T34075">Invitrogen Tubulin Tracker Green dye</a>, mitochondria are labeled with <a href="/order/catalog/product/M7511">Invitrogen MitoTracker Orange dye</a>, plasma membrane is labeled with <a href="/order/catalog/product/C10046">Invitrogen CellMask Deep Red dye</a>, and nucleus is stained blue with <a href="/order/catalog/product/62249">Hoechst 33342 reagent</a>. Image was taken with a Zeiss ELYRA S.1 microscope using Structured Illumination Microscopy (SIM). Image is provided to Thermo Fisher Scientific courtesy of M. Sauer and L. Behringer-Pließ, Biocenter University Wuerzburg.</small></p></div></div><div class="item"><img src="/content/dam/LifeTech/global/life-sciences/cellanalysis/Images/four-plex-image-U2OS-live-cells.png" alt="Four-plex image of U2OS live cells."><div class="carousel-caption"><p><small><b>Four-plex image of U2OS live cells.</b> Tubulin is labeled with <a href="/order/catalog/product/T34075">Tubulin Tracker Green dye</a>, mitochondria are labeled with <a href="/order/catalog/product/M7511">MitoTracker Orange dye</a>, plasma membrane is labeled with <a href="/order/catalog/product/C10046">CellMask Deep Red dye</a>, and nuclei are stained blue with <a href="/order/catalog/product/62249">Hoechst 33342 reagent</a>. Image was taken with an ELYRA S.1 microscope using Structured Illumination Microscopy (SIM). Image is provided to Thermo Fisher Scientific courtesy of M. Sauer and L. Behringer-Pließ, Biocenter University Wuerzburg.</small></p></div></div><div class="item"><img src="/content/dam/LifeTech/global/life-sciences/cellanalysis/Images/transduced-hela-cells-1.jpg" alt="HeLa cells were transduced with Invitrogen CellLight Mitochondria-RFP red dye and CellLight Talin-GFP green dye for 24 hours, then labeled with Invitrogen NucBlue Live ReadyProbes Reagent-Hoechst 33342 for 15 minutes."><div class="carousel-caption"><p><small><b>HeLa cells were transduced with <a href="/order/catalog/product/C10505">Invitrogen CellLight Mitochondria-RFP red dye</a> and <a href="/order/catalog/product/C10611">CellLight Talin-GFP green dye</a> for 24 hours,</b> then labeled with <a href="/order/catalog/product/R37605">Invitrogen NucBlue Live ReadyProbes Reagent-Hoechst 33342</a> for 15 minutes. For photobleach protection, cells were incubated with <a href="/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/mounting-medium-antifades/prolong-live-antifade.html">Invitrogen ProLong Live Antifade Reagent</a> for 90 minutes before imaging on an <a href="/sg/en/home/life-science/cell-analysis/cellular-imaging/evos-cell-imaging-systems.html">Invitrogen EVOS cell imaging system</a>.</small></p></div></div><div class="item"><img src="/content/dam/LifeTech/global/life-sciences/cellanalysis/Images/three-plex-image-COS7-live-cell.png" alt="Three-plex image of COS7 live cell."><div class="carousel-caption"><p><small><b>Three-plex image of COS7 live cell.</b> Tubulin is labeled with <a href="/order/catalog/product/T34075">Tubulin Tracker Green dye</a>, plasma membrane is labeled with <a href="/order/catalog/product/C10045">CellMask Orange dye</a>, and nucleus is stained blue with <a href="/order/catalog/product/62249">Hoechst 33342 reagent</a> . Image was taken using an ELYRA S.1 microscope using Structured Illumination Microscopy (SIM). Image is provided to Thermo Fisher Scientific courtesy of M. Sauer and L. Behringer-Pließ, Biocenter University Wuerzburg.</small></p></div></div><div class="item"><img src="/content/dam/LifeTech/global/life-sciences/cellanalysis/Images/transduced-hela-cells-2.jpg" alt="HeLa cells were transduced with CellLight Mitochondria-RFP red dye and CellLight Talin-GFP green dye for 24 hours, then labeled with NucBlue Live ReadyProbes Reagent-Hoechst 33342 for 15 minutes."><div class="carousel-caption"><p><small><b>HeLa cells were transduced with <a href="/order/catalog/product/C10505">CellLight Mitochondria-RFP red dye</a> and <a href="/order/catalog/product/C10611">CellLight Talin-GFP green dye</a> for 24 hours,</b> then labeled with <a href="/order/catalog/product/R37605">NucBlue Live ReadyProbes Reagent-Hoechst 33342</a> for 15 minutes. For photobleach protection, cells were incubated with <a href="/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/mounting-medium-antifades/prolong-live-antifade.html">ProLong Live Antifade Reagent</a> for 90 minutes before imaging on an <a href="/sg/en/home/life-science/cell-analysis/cellular-imaging/evos-cell-imaging-systems.html">EVOS cell imaging system</a>.</small></p></div></div></div><a class="carousel-control left" href="#5steps-gallery" data-slide="prev">&lsaquo;</a><a class="carousel-control right" href="#5steps-gallery" data-slide="next">&rsaquo;</a></div></div><div class="heading section"><h3> Video gallery for live-cell imaging </h3></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt3"><div class="parsys_column cq-colctrl-lt3-c0"><div container-id="cq-colctrl-lt3" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsysimage_404a"><a href="https://players.brightcove.net/3663210762001/default_default/index.html?videoId=6335045105112" target='_blank'><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_404a/foregroundimg.img.full.high.jpg/1738165970600.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_404a/foregroundimg.img.full.high.jpg/1738165970600.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_404a/foregroundimg.img.320.high.jpg/1738165970600.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_404a/foregroundimg.img.480.high.jpg/1738165970600.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_404a/foregroundimg.img.320.medium.jpg/1738165970600.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_404a/foregroundimg.img.620.high.jpg/1738165970600.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_404a/foregroundimg.img.320.low.jpg/1738165970600.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_404a/foregroundimg.img.480.medium.jpg/1738165970600.jpg" alt='Cell stained with CellRox Green in green and TMRM in red' title='Live-cell imaging of oxidative stress and mitochondrial function '></picture><style> #cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsysimage_404a img{ width:100%; max-width: 400px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsysimage_404a .image-contain" + "," + "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsysimage_404a .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a></div></div></div><div container-id="cq-colctrl-lt3" comp-id="A2"><div class="text parbase section"><p>U2OS cells treated with 50 mM menadione. <a></a><a href="/order/catalog/product/T668">Tetramethylrhodamine, Methyl Ester, Perchlorate (TMRM) (Cat. No. T668)</a> mitochondrial potential indicator and fades with oxidative stress as indicated with <a></a><a href="/order/catalog/product/C10444">CellROX Green reagent (Cat. No. C10444)</a>.</p></div></div></div><div class="parsys_column cq-colctrl-lt3-c1"><div container-id="cq-colctrl-lt3" comp-id="B1"><div class="image parbase section"><div id="cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsysimage_516c"><a href="https://players.brightcove.net/3663210762001/default_default/index.html?videoId=6335046695112"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_516c/foregroundimg.img.full.high.jpg/1738165970642.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_516c/foregroundimg.img.full.high.jpg/1738165970642.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_516c/foregroundimg.img.320.high.jpg/1738165970642.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_516c/foregroundimg.img.480.high.jpg/1738165970642.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_516c/foregroundimg.img.320.medium.jpg/1738165970642.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_516c/foregroundimg.img.620.high.jpg/1738165970642.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_516c/foregroundimg.img.320.low.jpg/1738165970642.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_516c/foregroundimg.img.480.medium.jpg/1738165970642.jpg" alt='Hippocampal neurons labeled with Tubulin Tracker Deep Red (Cat. No. T34076)' title='Hippocampal neurons '></picture><style> #cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsysimage_516c img{ width:100%; max-width: 400px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsysimage_516c .image-contain" + "," + "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsysimage_516c .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a></div></div></div><div container-id="cq-colctrl-lt3" comp-id="B2"><div class="text parbase section"><p>Hippocampal neurons labeled with <a></a><a href="/order/catalog/product/T34076">Tubulin Tracker Deep Red (Cat. No. T34076)</a>.</p></div></div></div><div class="parsys_column cq-colctrl-lt3-c2"><div container-id="cq-colctrl-lt3" comp-id="C1"><div class="image parbase section"><div id="cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsysimage_5d46"><a href="https://players.brightcove.net/3663210762001/default_default/index.html?videoId=6335042573112"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_5d46/foregroundimg.img.full.high.jpg/1738165970677.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_5d46/foregroundimg.img.full.high.jpg/1738165970677.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_5d46/foregroundimg.img.320.high.jpg/1738165970677.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_5d46/foregroundimg.img.480.high.jpg/1738165970677.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_5d46/foregroundimg.img.320.medium.jpg/1738165970677.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_5d46/foregroundimg.img.620.high.jpg/1738165970677.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_5d46/foregroundimg.img.320.low.jpg/1738165970677.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_5d46/foregroundimg.img.480.medium.jpg/1738165970677.jpg" alt='Cell transfected with CellLight Tubulin-GFP in green and CellLight Actin-RFP in red' title='Live-cell imaging of F-actin '></picture><style> #cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsysimage_5d46 img{ width:100%; max-width: 400px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsysimage_5d46 .image-contain" + "," + "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsysimage_5d46 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a></div></div></div><div container-id="cq-colctrl-lt3" comp-id="C2"><div class="text parbase section"><p>U2OS cells transfected with <a></a><a href="/order/catalog/product/C10613">CellLight Tubulin-GFP (Cat. No. C10613)</a>; <a></a><a href="/order/catalog/product/C10583">CellLight Actin-RFP (Cat. No. C10583)</a>.</p></div></div></div></div><div style="clear:both"></div><div class="heading section"><h2 id="imaging-resources"> Live-cell imaging resources </h2></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt3"><div class="parsys_column cq-colctrl-lt3-c0"><div container-id="cq-colctrl-lt3" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsysimage_d311"><a href="https://assets.thermofisher.com/TFS-Assets/BID/brochures/5-steps-to-live-cell-imaging.pdf" target='_blank'><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_d311/foregroundimg.img.full.high.jpg/1738165970723.jpg' media="(min-width: 1px)"><img class='image-auto' srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_d311/foregroundimg.img.full.high.jpg/1738165970723.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_d311/foregroundimg.img.320.high.jpg/1738165970723.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_d311/foregroundimg.img.480.high.jpg/1738165970723.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_d311/foregroundimg.img.320.medium.jpg/1738165970723.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_d311/foregroundimg.img.620.high.jpg/1738165970723.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_d311/foregroundimg.img.320.low.jpg/1738165970723.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_d311/foregroundimg.img.480.medium.jpg/1738165970723.jpg" alt='Guide cover photo' title='Live cell imaging 5-step guide'></picture><style> #cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsysimage_d311 img{ width:100%; max-width: 380px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsysimage_d311 .image-contain" + "," + "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsysimage_d311 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a></div></div></div></div><div class="parsys_column cq-colctrl-lt3-c1"><div container-id="cq-colctrl-lt3" comp-id="B1"><div class="image parbase section"><div id="cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsysimage_79a0"><a href="/sg/en/home/global/forms/life-science/live-cell-imaging-reagent-wheel-request.html"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_79a0/foregroundimg.img.full.high.jpg/1738165970759.jpg' media="(min-width: 1px)"><img class='image-auto' srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_79a0/foregroundimg.img.full.high.jpg/1738165970759.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_79a0/foregroundimg.img.320.high.jpg/1738165970759.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_79a0/foregroundimg.img.480.high.jpg/1738165970759.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_79a0/foregroundimg.img.320.medium.jpg/1738165970759.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_79a0/foregroundimg.img.620.high.jpg/1738165970759.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_79a0/foregroundimg.img.320.low.jpg/1738165970759.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_79a0/foregroundimg.img.480.medium.jpg/1738165970759.jpg" alt='Reagents wheel image' title='Live-cell imaging reagents wheel'></picture><style> #cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsysimage_79a0 img{ width:100%; max-width: 380px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsysimage_79a0 .image-contain" + "," + "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsysimage_79a0 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a></div></div></div></div><div class="parsys_column cq-colctrl-lt3-c2"><div container-id="cq-colctrl-lt3" comp-id="C1"><div class="image parbase section"><div id="cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsysimage_5056"><a href="/sg/en/home/global/forms/imaging-protocols-handbook.html.html"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_5056/foregroundimg.img.full.high.jpg/1738165970790.jpg' media="(min-width: 1px)"><img class='image-auto' srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_5056/foregroundimg.img.full.high.jpg/1738165970790.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_5056/foregroundimg.img.320.high.jpg/1738165970790.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_5056/foregroundimg.img.480.high.jpg/1738165970790.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_5056/foregroundimg.img.320.medium.jpg/1738165970790.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_5056/foregroundimg.img.620.high.jpg/1738165970790.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_5056/foregroundimg.img.320.low.jpg/1738165970790.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/microscopy-reagents-and-media/live-cell-imaging-reagents/jcr:content/MainParsys/image_5056/foregroundimg.img.480.medium.jpg/1738165970790.jpg" alt='Handbook cover' title='Imaging protocol handbook'></picture><style> #cq-image-jsp-53c47662-be51-4166-98a2-30c652000e1eMainParsysimage_5056 img{ width:100%; max-width: 380px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsysimage_5056 .image-contain" + "," + "#cq-image-jsp-"+"53c47662-be51-4166-98a2-30c652000e1eMainParsysimage_5056 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a></div></div></div></div></div><div style="clear:both"></div><div class="heading section"><h3> For additional information and data, please see the following posters: </h3></div><div class="text parbase section"><p><span class="icon-lt-pdf"></span><a class="cta-link" href="https://assets.thermofisher.com/TFS-Assets/BID/posters/sensors-caspase-superoxide-poster.pdf" target="_blank">Poster: New generation sensors for caspase activation and mitochondrial superoxide in live cell microscopy</a><br><span class="icon-lt-pdf"></span><a class="cta-link" href="https://assets.thermofisher.com/TFS-Assets/BID/posters/new-improved-cellular-health-evaluation-microplate-reader-assays-poster.pdf" target="_blank">Poster: New and improved cellular health evaluation of 2D and 3D cellular models using microplate reader assays</a><br><span class="icon-lt-pdf"></span><a class="cta-link" href="https://assets.thermofisher.com/TFS-Assets/BID/posters/hypoxia-measurements-fluorescence-microscopy-imaging-poster.pdf" target="_blank">Poster: Hypoxia measurements in live and fixed cells using fluorescence microscopy and high-content imaging</a><br><span class="icon-lt-pdf"></span><a class="cta-link" href="https://assets.thermofisher.com/TFS-Assets/BID/posters/evaluation-cellular-senescence-fluorescence-characterization-poster.pdf" target="_blank">Poster: Evaluation of Cellular Senescence through Fluorescence Characterization</a><b></b></p><p>*Incucyte® Live-Cell Analysis System is trademarked or registered trademarks of Essen BioScience. Incucyte, Essen BioScience, and all names of Essen BioScience products are registered trademarks and the property of Essen BioScience unless otherwise specified. Essen BioScience is a Sartorius Company. MuviCyte™ live-cell imaging system is trademarked or registered trademarked of PerkinElmer. THUNDER Imager Live Cell & 3D Assay and MICA Microhub Microscope are sold by Leica Microsystems CMS GmbH.</p><p> </p></div></div><div><div class="MainInheritParsys iparsys parsys"><div class="section"><div class="new"></div></div><div class="iparys_inherited"><div class="MainInheritParsys iparsys parsys"><div class="reference parbase section"><div class="cq-dd-paragraph"><div class="ltrawhtml_d1d9 LTRawHTML"><style> /* Remove horizontal scroll on hero banner table */ .wellPageHeading .well-container .table-component-wrapper table {width:100% !important;} .content.wide-template .container-wrap.template-2-column .span3.sidebar > .container-leftnav {margin-right:20px;} /*.railsection {padding-right:10px;}*/ .textimage {margin-bottom:0px;} /* Label display for author */ .ux-tool-label { display: none; border-left: 50px #dcdcdc solid; padding-left: 10px; font-style: italic; font-size: 14px; } .cq-wcm-edit .ux-tool-label {display: block;} .wellPageHeading h1 b, .wellPageHeading h1 strong {font-weight:normal !important;} .wellPageHeading table td > * {text-shadow: none !important;} </style><div class="ux-tool-label ">Stylesheet for Classic Wide Template adjustments</div></div></div></div><div class="reference parbase section"><div class="cq-dd-paragraph"><div class="ltrawhtml_a06 LTRawHTML"><style> /* Hero Banner Dark Grandient */ .wellPageHeading .well-content { background-image: linear-gradient(-45deg,rgba(68,68,68,0),rgba(0,0,0,.686)); } /* Label display for author */ .ux-tool-label { display: none; border-left: 50px #dcdcdc solid; padding-left: 10px; font-style: italic; font-size: 14px; } .cq-wcm-edit .ux-tool-label {display: block;} </style><div class="ux-tool-label ">Hero banner dark gradient overlay</div></div></div></div><div class="reference parbase section"><div class="cq-dd-paragraph"><div class="text_72be text parbase"><p>For Research Use Only. 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