Search results for: Renaldas Urniezius

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class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 2</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: Renaldas Urniezius</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2</span> Development and Investigation of Efficient Substrate Feeding and Dissolved Oxygen Control Algorithms for Scale-Up of Recombinant E. coli Cultivation Process</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Vytautas%20Galvanauskas">Vytautas Galvanauskas</a>, <a href="https://publications.waset.org/abstracts/search?q=Rimvydas%20Simutis"> Rimvydas Simutis</a>, <a href="https://publications.waset.org/abstracts/search?q=Donatas%20Levisauskas"> Donatas Levisauskas</a>, <a href="https://publications.waset.org/abstracts/search?q=Vykantas%20Grincas"> Vykantas Grincas</a>, <a href="https://publications.waset.org/abstracts/search?q=Renaldas%20Urniezius"> Renaldas Urniezius</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The paper deals with model-based development and implementation of efficient control strategies for recombinant protein synthesis in fed-batch E.coli cultivation processes. Based on experimental data, a kinetic dynamic model for cultivation process was developed. This model was used to determine substrate feeding strategies during the cultivation. The proposed feeding strategy consists of two phases – biomass growth phase and recombinant protein production phase. In the first process phase, substrate-limited process is recommended when the specific growth rate of biomass is about 90-95% of its maximum value. This ensures reduction of glucose concentration in the medium, improves process repeatability, reduces the development of secondary metabolites and other unwanted by-products. The substrate limitation can be enhanced to satisfy restriction on maximum oxygen transfer rate in the bioreactor and to guarantee necessary dissolved carbon dioxide concentration in culture media. In the recombinant protein production phase, the level of substrate limitation and specific growth rate are selected within the range to enable optimal target protein synthesis rate. To account for complex process dynamics, to efficiently exploit the oxygen transfer capability of the bioreactor, and to maintain the required dissolved oxygen concentration, adaptive control algorithms for dissolved oxygen control have been proposed. The developed model-based control strategies are useful in scale-up of cultivation processes and accelerate implementation of innovative biotechnological processes for industrial applications. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=adaptive%20algorithms" title="adaptive algorithms">adaptive algorithms</a>, <a href="https://publications.waset.org/abstracts/search?q=model-based%20control" title=" model-based control"> model-based control</a>, <a href="https://publications.waset.org/abstracts/search?q=recombinant%20E.%20coli" title=" recombinant E. coli"> recombinant E. coli</a>, <a href="https://publications.waset.org/abstracts/search?q=scale-up%20of%20bioprocesses" title=" scale-up of bioprocesses"> scale-up of bioprocesses</a> </p> <a href="https://publications.waset.org/abstracts/88066/development-and-investigation-of-efficient-substrate-feeding-and-dissolved-oxygen-control-algorithms-for-scale-up-of-recombinant-e-coli-cultivation-process" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/88066.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">258</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1</span> Automatic Differential Diagnosis of Melanocytic Skin Tumours Using Ultrasound and Spectrophotometric Data</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Kristina%20Sakalauskiene">Kristina Sakalauskiene</a>, <a href="https://publications.waset.org/abstracts/search?q=Renaldas%20Raisutis"> Renaldas Raisutis</a>, <a href="https://publications.waset.org/abstracts/search?q=Gintare%20Linkeviciute"> Gintare Linkeviciute</a>, <a href="https://publications.waset.org/abstracts/search?q=Skaidra%20Valiukeviciene"> Skaidra Valiukeviciene</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Cutaneous melanoma is a melanocytic skin tumour, which has a very poor prognosis while is highly resistant to treatment and tends to metastasize. Thickness of melanoma is one of the most important biomarker for stage of disease, prognosis and surgery planning. In this study, we hypothesized that the automatic analysis of spectrophotometric images and high-frequency ultrasonic 2D data can improve differential diagnosis of cutaneous melanoma and provide additional information about tumour penetration depth. This paper presents the novel complex automatic system for non-invasive melanocytic skin tumour differential diagnosis and penetration depth evaluation. The system is composed of region of interest segmentation in spectrophotometric images and high-frequency ultrasound data, quantitative parameter evaluation, informative feature extraction and classification with linear regression classifier. The segmentation of melanocytic skin tumour region in ultrasound image is based on parametric integrated backscattering coefficient calculation. The segmentation of optical image is based on Otsu thresholding. In total 29 quantitative tissue characterization parameters were evaluated by using ultrasound data (11 acoustical, 4 shape and 15 textural parameters) and 55 quantitative features of dermatoscopic and spectrophotometric images (using total melanin, dermal melanin, blood and collagen SIAgraphs acquired using spectrophotometric imaging device SIAscope). In total 102 melanocytic skin lesions (including 43 cutaneous melanomas) were examined by using SIAscope and ultrasound system with 22 MHz center frequency single element transducer. The diagnosis and Breslow thickness (pT) of each MST were evaluated during routine histological examination after excision and used as a reference. The results of this study have shown that automatic analysis of spectrophotometric and high frequency ultrasound data can improve non-invasive classification accuracy of early-stage cutaneous melanoma and provide supplementary information about tumour penetration depth. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cutaneous%20melanoma" title="cutaneous melanoma">cutaneous melanoma</a>, <a href="https://publications.waset.org/abstracts/search?q=differential%20diagnosis" title=" differential diagnosis"> differential diagnosis</a>, <a href="https://publications.waset.org/abstracts/search?q=high-frequency%20ultrasound" title=" high-frequency ultrasound"> high-frequency ultrasound</a>, <a href="https://publications.waset.org/abstracts/search?q=melanocytic%20skin%20tumours" title=" melanocytic skin tumours"> melanocytic skin tumours</a>, <a href="https://publications.waset.org/abstracts/search?q=spectrophotometric%20imaging" title=" spectrophotometric imaging"> spectrophotometric imaging</a> </p> <a href="https://publications.waset.org/abstracts/94893/automatic-differential-diagnosis-of-melanocytic-skin-tumours-using-ultrasound-and-spectrophotometric-data" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/94893.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">273</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" 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Search results for: Renaldas Urniezius