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spectrophotometric analysis</title> <meta name="description" content="Search results for: spectrophotometric analysis"> <meta name="keywords" content="spectrophotometric analysis"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> 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action="https://publications.waset.org/abstracts/search"> <div id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="spectrophotometric analysis"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 27940</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: spectrophotometric analysis</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">27940</span> Spectrophotometric Evaluation of Custom Microalgae-Based Bioink Formulations for Optimized Green Bioprinting</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Olubusuyi%20Ayowole">Olubusuyi Ayowole</a>, <a href="https://publications.waset.org/abstracts/search?q=Bashir%20Khoda"> Bashir Khoda</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Green bioprinting, from the context of merging 3D bioprinting with microalgae cell organization, holds promise for industrial-scale optimization. This study employs spectrophotometric analysis to explore post-bioprinting cell growth density variation within hybrid hydrogel biomaterial scaffolds. Three hydrogel biomaterials—Alginic acid sodium salt (ALGINATE), Nanofibrillated Cellulose (NFC) – TEMPO, and CarboxyMethyl Cellulose (CMC)—are chosen for their scaffolding capabilities. Bioink development and analysis of their impact on cell proliferation and morphology are conducted. Chlorella microalgae cell growth within hydrogel compositions is probed using absorbance measurements, with additional assessment of shear thinning properties. Notably, NFC exhibits reduced shear thinning compared to CMC. Results reveal that while mono-hydrogel substrates with pronounced adhesion inhibit Chlorella cell proliferation, Alginate fosters increased cell concentration alongside a slight viscosity rise. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=green%20bioprinting" title="green bioprinting">green bioprinting</a>, <a href="https://publications.waset.org/abstracts/search?q=3d%20bioprinting" title=" 3d bioprinting"> 3d bioprinting</a>, <a href="https://publications.waset.org/abstracts/search?q=microalgae%20cell" title=" microalgae cell"> microalgae cell</a>, <a href="https://publications.waset.org/abstracts/search?q=hybrid%20hydrogel%20scaffolds" title=" hybrid hydrogel scaffolds"> hybrid hydrogel scaffolds</a>, <a href="https://publications.waset.org/abstracts/search?q=spectrophotometric%20analysis" title=" spectrophotometric analysis"> spectrophotometric analysis</a>, <a href="https://publications.waset.org/abstracts/search?q=bioink%20development" title=" bioink development"> bioink development</a>, <a href="https://publications.waset.org/abstracts/search?q=shear%20thinning%20properties" title=" shear thinning properties"> shear thinning properties</a> </p> <a href="https://publications.waset.org/abstracts/188298/spectrophotometric-evaluation-of-custom-microalgae-based-bioink-formulations-for-optimized-green-bioprinting" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/188298.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">29</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">27939</span> Automatic Differential Diagnosis of Melanocytic Skin Tumours Using Ultrasound and Spectrophotometric Data</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Kristina%20Sakalauskiene">Kristina Sakalauskiene</a>, <a href="https://publications.waset.org/abstracts/search?q=Renaldas%20Raisutis"> Renaldas Raisutis</a>, <a href="https://publications.waset.org/abstracts/search?q=Gintare%20Linkeviciute"> Gintare Linkeviciute</a>, <a href="https://publications.waset.org/abstracts/search?q=Skaidra%20Valiukeviciene"> Skaidra Valiukeviciene</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Cutaneous melanoma is a melanocytic skin tumour, which has a very poor prognosis while is highly resistant to treatment and tends to metastasize. Thickness of melanoma is one of the most important biomarker for stage of disease, prognosis and surgery planning. In this study, we hypothesized that the automatic analysis of spectrophotometric images and high-frequency ultrasonic 2D data can improve differential diagnosis of cutaneous melanoma and provide additional information about tumour penetration depth. This paper presents the novel complex automatic system for non-invasive melanocytic skin tumour differential diagnosis and penetration depth evaluation. The system is composed of region of interest segmentation in spectrophotometric images and high-frequency ultrasound data, quantitative parameter evaluation, informative feature extraction and classification with linear regression classifier. The segmentation of melanocytic skin tumour region in ultrasound image is based on parametric integrated backscattering coefficient calculation. The segmentation of optical image is based on Otsu thresholding. In total 29 quantitative tissue characterization parameters were evaluated by using ultrasound data (11 acoustical, 4 shape and 15 textural parameters) and 55 quantitative features of dermatoscopic and spectrophotometric images (using total melanin, dermal melanin, blood and collagen SIAgraphs acquired using spectrophotometric imaging device SIAscope). In total 102 melanocytic skin lesions (including 43 cutaneous melanomas) were examined by using SIAscope and ultrasound system with 22 MHz center frequency single element transducer. The diagnosis and Breslow thickness (pT) of each MST were evaluated during routine histological examination after excision and used as a reference. The results of this study have shown that automatic analysis of spectrophotometric and high frequency ultrasound data can improve non-invasive classification accuracy of early-stage cutaneous melanoma and provide supplementary information about tumour penetration depth. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cutaneous%20melanoma" title="cutaneous melanoma">cutaneous melanoma</a>, <a href="https://publications.waset.org/abstracts/search?q=differential%20diagnosis" title=" differential diagnosis"> differential diagnosis</a>, <a href="https://publications.waset.org/abstracts/search?q=high-frequency%20ultrasound" title=" high-frequency ultrasound"> high-frequency ultrasound</a>, <a href="https://publications.waset.org/abstracts/search?q=melanocytic%20skin%20tumours" title=" melanocytic skin tumours"> melanocytic skin tumours</a>, <a href="https://publications.waset.org/abstracts/search?q=spectrophotometric%20imaging" title=" spectrophotometric imaging"> spectrophotometric imaging</a> </p> <a href="https://publications.waset.org/abstracts/94893/automatic-differential-diagnosis-of-melanocytic-skin-tumours-using-ultrasound-and-spectrophotometric-data" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/94893.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">270</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">27938</span> A Comparison between Reagents Extracted from Tree Leaves for Spectrophotometric Determination of Hafnium(IV)</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=A.%20Boveiri%20Monji">A. Boveiri Monji</a>, <a href="https://publications.waset.org/abstracts/search?q=H.%20Yousefnia"> H. Yousefnia</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20Zolghadri"> S. Zolghadri</a>, <a href="https://publications.waset.org/abstracts/search?q=B.%20Salimi"> B. Salimi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The main goal of this paper was to make use of green reagents as a substitute of perilous synthetic reagents and organic solvents for spectrophotometric determination of hafnium(IV). The extracts taken from six different kinds of tree leaves including <em>Acer negundo, Ficus carica, Cerasus avium, Chimonanthus, Salix babylonica</em> and <em>Pinus brutia</em>, were applied as green reagents for the experiments. In 6-M hydrochloric acid, hafnium reacted with the reagent to form a yellow product and showed maximum absorbance at 421 nm. Among tree leaves, <em>Chimonanthus</em> showed satisfactory results with a molar absorptivity value of 0.61 × 10<sup>4</sup> l mol<sup>-1</sup> cm<sup>-1</sup> and the method was linear in the 0.3-9 µg mL<sup> -1</sup> concentration range. The detection limit value was 0.064 µg mL<sup>-1</sup>. The proposed method was simple, low cost, clean, and selective. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=hafnium" title="hafnium">hafnium</a>, <a href="https://publications.waset.org/abstracts/search?q=spectrophotometric%20determination" title=" spectrophotometric determination"> spectrophotometric determination</a>, <a href="https://publications.waset.org/abstracts/search?q=synthetic%20reagents" title=" synthetic reagents"> synthetic reagents</a>, <a href="https://publications.waset.org/abstracts/search?q=tree%20leaves" title=" tree leaves"> tree leaves</a> </p> <a href="https://publications.waset.org/abstracts/88856/a-comparison-between-reagents-extracted-from-tree-leaves-for-spectrophotometric-determination-of-hafniumiv" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/88856.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">188</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">27937</span> A Green Method for Selective Spectrophotometric Determination of Hafnium(IV) with Aqueous Extract of Ficus carica Tree Leaves</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=A.%20Boveiri%20Monji">A. Boveiri Monji</a>, <a href="https://publications.waset.org/abstracts/search?q=H.%20Yousefnia"> H. Yousefnia</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Haji%20Hosseini"> M. Haji Hosseini</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20Zolghadri"> S. Zolghadri</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A clean spectrophotometric method for the determination of hafnium by using a green reagent, acidic extract of <em>Ficus carica</em> tree leaves is developed. In 6-M hydrochloric acid, hafnium reacts with this reagent to form a yellow product. The formed product shows maximum absorbance at 421 nm with a molar absorptivity value of 0.28 × 104 l mol⁻¹ cm⁻¹, and the method was linear in the 2-11 µg ml⁻¹ concentration range. The detection limit value was found to be 0.312 µg ml⁻¹. Except zirconium and iron, the selectivity was good, and most of the ions did not show any significant spectral interference at concentrations up to several hundred times. The proposed method was green, simple, low cost, and selective. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=spectrophotometric%20determination" title="spectrophotometric determination">spectrophotometric determination</a>, <a href="https://publications.waset.org/abstracts/search?q=Ficus%20caricatree%20leaves" title=" Ficus caricatree leaves"> Ficus caricatree leaves</a>, <a href="https://publications.waset.org/abstracts/search?q=synthetic%20reagents" title=" synthetic reagents"> synthetic reagents</a>, <a href="https://publications.waset.org/abstracts/search?q=hafnium" title=" hafnium"> hafnium</a> </p> <a href="https://publications.waset.org/abstracts/88855/a-green-method-for-selective-spectrophotometric-determination-of-hafniumiv-with-aqueous-extract-of-ficus-carica-tree-leaves" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/88855.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">209</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">27936</span> Spectrophotometric Determination of Phenylephrine Hydrochloride by Coupling with Diazotized 2,4-Dinitroaniline</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sulaiman%20Gafar%20Muhamad">Sulaiman Gafar Muhamad</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A rapid spectrophotometric method for the micro-determination of phenylephrine-HCl (PHE) has been developed. The proposed method involves the coupling of phenylephrine-HCl with diazotized 2,4-dinitroaniline in alkaline medium at λmax 455 nm. Under the present optimum condition, Beer’s law was obeyed in the range of 1.0-20 μg/ml of PHE with molar absorptivity of 1.915 ×104 l. mol-1.cm-1, with a relative error of 0.015 and a relative standard deviation of 0.024%. The current method has been applied successfully to estimate phenylephrine-HCl in pharmaceutical preparations (nose drop and syrup). <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=diazo-coupling" title="diazo-coupling">diazo-coupling</a>, <a href="https://publications.waset.org/abstracts/search?q=2" title=" 2"> 2</a>, <a href="https://publications.waset.org/abstracts/search?q=4-dinitroaniline" title="4-dinitroaniline">4-dinitroaniline</a>, <a href="https://publications.waset.org/abstracts/search?q=phenylephrine-HCl" title=" phenylephrine-HCl"> phenylephrine-HCl</a>, <a href="https://publications.waset.org/abstracts/search?q=spectrophotometry" title=" spectrophotometry"> spectrophotometry</a> </p> <a href="https://publications.waset.org/abstracts/53006/spectrophotometric-determination-of-phenylephrine-hydrochloride-by-coupling-with-diazotized-24-dinitroaniline" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/53006.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">258</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">27935</span> Spectrophotometric Determination of 5-Aminosalicylic Acid in Pharmaceutical Samples </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Chand%20Pasha">Chand Pasha</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A Simple, accurate and precise spectrophotometric method for the quantitative analysis of determination of 5-aminosalicylic acid is described. This method is based on the reaction of 5-aminosalicylic acid with nitrite in acid medium to form diazonium ion, which is coupled with acetylacetone in basic medium to form azo dyes, which shows absorption maxima at 470 nm. The method obeys Beer’s law in the concentration range of 0.5-11.2 gml-1 of 5-aminosalicylic acid with acetylacetone. The molar absorptivity and Sandell’s sensitivity of 5-aminosalicylic acid -acetylacetone azo dye is 2.672 ×104 lmol-1cm-1, 5.731 × 10-3 gcm-2 respectively. The dye formed is stable for 10 hrs. The optimum reaction conditions and other analytical parameters are evaluated. Interference due to foreign organic compounds have been investigated. The method has been successfully applied to the determination of 5-aminosalicylic acid in pharmaceutical samples. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=spectrophotometry" title="spectrophotometry">spectrophotometry</a>, <a href="https://publications.waset.org/abstracts/search?q=diazotization" title=" diazotization"> diazotization</a>, <a href="https://publications.waset.org/abstracts/search?q=mesalazine" title=" mesalazine"> mesalazine</a>, <a href="https://publications.waset.org/abstracts/search?q=nitrite" title=" nitrite"> nitrite</a>, <a href="https://publications.waset.org/abstracts/search?q=acetylacetone" title=" acetylacetone"> acetylacetone</a> </p> <a href="https://publications.waset.org/abstracts/88694/spectrophotometric-determination-of-5-aminosalicylic-acid-in-pharmaceutical-samples" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/88694.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">189</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">27934</span> Spectrophotometric Methods for Simultaneous Determination of Binary Mixture of Amlodipine Besylate and Atenolol Based on Dual Wavelength</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Nesrine%20T.%20Lamie">Nesrine T. Lamie</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Four, accurate, precise, and sensitive spectrophotometric methods are developed for the simultaneous determination of a binary mixture containing amlodipine besylate (AM) and atenolol (AT) where AM is determined at its λmax 360 nm (0D), while atenolol can be determined by different methods. Method (A) is absorpotion factor (AFM). Method (B) is the new Ratio Difference method(RD) which measures the difference in amplitudes between 210 and 226 nm of ratio spectrum., Method (C) is novel constant center spectrophotometric method (CC) Method (D) is mean centering of the ratio spectra (MCR) at 284 nm. The calibration curve is linear over the concentration range of 10–80 and 4–40 μg/ml for AM and AT, respectively. These methods are tested by analyzing synthetic mixtures of the cited drugs and they are applied to their commercial pharmaceutical preparation. The validity of results was assessed by applying standard addition technique. The results obtained were found to agree statistically with those obtained by a reported method, showing no significant difference with respect to accuracy and precision. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=amlodipine" title="amlodipine">amlodipine</a>, <a href="https://publications.waset.org/abstracts/search?q=atenolol" title=" atenolol"> atenolol</a>, <a href="https://publications.waset.org/abstracts/search?q=absorption%20factor" title=" absorption factor"> absorption factor</a>, <a href="https://publications.waset.org/abstracts/search?q=constant%20center" title=" constant center"> constant center</a>, <a href="https://publications.waset.org/abstracts/search?q=mean%20centering" title=" mean centering"> mean centering</a>, <a href="https://publications.waset.org/abstracts/search?q=ratio%20difference" title=" ratio difference"> ratio difference</a> </p> <a href="https://publications.waset.org/abstracts/24767/spectrophotometric-methods-for-simultaneous-determination-of-binary-mixture-of-amlodipine-besylate-and-atenolol-based-on-dual-wavelength" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/24767.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">304</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">27933</span> A Spectrophotometric Method for the Determination of Folic Acid - A Vitamin B9 in Pharmaceutical Dosage Samples</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Chand%20Pasha">Chand Pasha</a>, <a href="https://publications.waset.org/abstracts/search?q=Yasser%20Turki%20Alharbi"> Yasser Turki Alharbi</a>, <a href="https://publications.waset.org/abstracts/search?q=Krasamira%20Stancheva"> Krasamira Stancheva</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A simple spectrophotometric method for the determination of folic acid in pharmaceutical dosage samples was developed. The method is based on the diazotization reaction of thiourea with sodium nitrite in acidic medium yields diazonium compounds, which is then coupled with folic acid in basic medium yields yellow coloured azo dyes. Beer’s Lamberts law is observed in the range 0.5 – 16.2 μgmL-1 at a maximum wavelength of 416nm. The molar absorbtivity, sandells sensitivity, linear regression equation and detection limit and quantitation limit were found to be 5.695×104 L mol-1cm-1, 7.752×10-3 g cm-2, y= 0.092x - 0.018, 0.687 g mL-1 and 2.083 g mL-1. This method successfully determined Folate in Pharmaceutical formulations. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=folic%20acid%20determination" title="folic acid determination">folic acid determination</a>, <a href="https://publications.waset.org/abstracts/search?q=spectrophotometry" title=" spectrophotometry"> spectrophotometry</a>, <a href="https://publications.waset.org/abstracts/search?q=diazotization" title=" diazotization"> diazotization</a>, <a href="https://publications.waset.org/abstracts/search?q=thiourea" title=" thiourea"> thiourea</a>, <a href="https://publications.waset.org/abstracts/search?q=pharmaceutical%20dosage%20samples" title=" pharmaceutical dosage samples"> pharmaceutical dosage samples</a> </p> <a href="https://publications.waset.org/abstracts/179739/a-spectrophotometric-method-for-the-determination-of-folic-acid-a-vitamin-b9-in-pharmaceutical-dosage-samples" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/179739.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">76</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">27932</span> Pd(II) Complex with 4-Bromo-2,6-Bis-Hydroxymethyl-Phenol and Nikotinamid: Synthesis and Spectral Analysis </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=%C3%96zlen%20Altun">Özlen Altun</a>, <a href="https://publications.waset.org/abstracts/search?q=Zeliha%20Yoru%C3%A7"> Zeliha Yoruç</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In the present study, the reactions involving 4-Bromo-2,6-bis-hydroxymethyl-phenol (BBHMP) and nikotinamide (NA) in the presence Pd (II) ion were investigated. Optimum conditions for the reactions were established as pH 7 and λ = 450 nm. According to absorbance measurements, the mole ratio of BBHMP : NA : Pd2+ was found as 1 : 2 : 2. As a result of physico-chemical, spectrophotometric and thermal analysis results, the reactions of BBHMP and NA with Pd (II) is complexation reactions and one molecule BBHMP and two molecules of NA react with two molecules of metal (II) ion. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=4-Bromo-2" title="4-Bromo-2">4-Bromo-2</a>, <a href="https://publications.waset.org/abstracts/search?q=6-bis-hydroxymethyl-phenol" title="6-bis-hydroxymethyl-phenol">6-bis-hydroxymethyl-phenol</a>, <a href="https://publications.waset.org/abstracts/search?q=nicotinamide" title=" nicotinamide"> nicotinamide</a>, <a href="https://publications.waset.org/abstracts/search?q=Pd%28II%29" title=" Pd(II)"> Pd(II)</a>, <a href="https://publications.waset.org/abstracts/search?q=spectral%20analysis" title=" spectral analysis"> spectral analysis</a>, <a href="https://publications.waset.org/abstracts/search?q=synthesis" title=" synthesis"> synthesis</a> </p> <a href="https://publications.waset.org/abstracts/82499/pdii-complex-with-4-bromo-26-bis-hydroxymethyl-phenol-and-nikotinamid-synthesis-and-spectral-analysis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/82499.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">165</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">27931</span> Solvent Extraction, Spectrophotometric Determination of Antimony(III) from Real Samples and Synthetic Mixtures Using O-Methylphenyl Thiourea as a Sensitive Reagent</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Shashikant%20R.%20Kuchekar">Shashikant R. Kuchekar</a>, <a href="https://publications.waset.org/abstracts/search?q=Shivaji%20D.%20Pulate"> Shivaji D. Pulate</a>, <a href="https://publications.waset.org/abstracts/search?q=Vishwas%20B.%20Gaikwad"> Vishwas B. Gaikwad</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A simple and selective method is developed for solvent extraction spectrophotometric determination of antimony(III) using O-Methylphenyl Thiourea (OMPT) as a sensitive chromogenic chelating agent. The basis of proposed method is formation of antimony(III)-OMPT complex was extracted with 0.0025 M OMPT in chloroform from aqueous solution of antimony(III) in 1.0 M perchloric acid. The absorbance of this complex was measured at 297 nm against reagent blank. Beer’s law was obeyed up to 15µg mL-1 of antimony(III). The Molar absorptivity and Sandell’s sensitivity of the antimony(III)-OMPT complex in chloroform are 16.6730 × 103 L mol-1 cm-1 and 0.00730282 µg cm-2 respectively. The stoichiometry of antimony(III)-OMPT complex was established from slope ratio method, mole ratio method and Job’s continuous variation method was 1:2. The complex was stable for more than 48 h. The interfering effect of various foreign ions was studied and suitable masking agents are used wherever necessary to enhance selectivity of the method. The proposed method is successfully applied for determination of antimony(III) from real samples alloy and synthetic mixtures. Repetition of the method was checked by finding relative standard deviation (RSD) for 10 determinations which was 0.42%. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=solvent%20extraction" title="solvent extraction">solvent extraction</a>, <a href="https://publications.waset.org/abstracts/search?q=antimony" title=" antimony"> antimony</a>, <a href="https://publications.waset.org/abstracts/search?q=spectrophotometry" title=" spectrophotometry"> spectrophotometry</a>, <a href="https://publications.waset.org/abstracts/search?q=real%20sample%20analysis" title=" real sample analysis"> real sample analysis</a> </p> <a href="https://publications.waset.org/abstracts/68993/solvent-extraction-spectrophotometric-determination-of-antimonyiii-from-real-samples-and-synthetic-mixtures-using-o-methylphenyl-thiourea-as-a-sensitive-reagent" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/68993.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">332</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">27930</span> Solvent Extraction and Spectrophotometric Determination of Palladium(II) Using P-Methylphenyl Thiourea as a Complexing Agent</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Shashikant%20R.%20Kuchekar">Shashikant R. Kuchekar</a>, <a href="https://publications.waset.org/abstracts/search?q=Somnath%20D.%20Bhumkar"> Somnath D. Bhumkar</a>, <a href="https://publications.waset.org/abstracts/search?q=Haribhau%20R.%20Aher"> Haribhau R. Aher</a>, <a href="https://publications.waset.org/abstracts/search?q=Bhaskar%20H.%20Zaware"> Bhaskar H. Zaware</a>, <a href="https://publications.waset.org/abstracts/search?q=Ponnadurai%20Ramasami"> Ponnadurai Ramasami</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A precise, sensitive, rapid and selective method for the solvent extraction, spectrophotometric determination of palladium(II) using para-methylphenyl thiourea (PMPT) as an extractant is developed. Palladium(II) forms yellow colored complex with PMPT which shows an absorption maximum at 300 nm. The colored complex obeys Beer’s law up to 7.0 µg ml<sup>-1</sup> of palladium. The molar absorptivity and Sandell’s sensitivity were found to be 8.486 x 10<sup>3</sup> l mol<sup>-1</sup>cm<sup>-1</sup> and 0.0125 μg cm<sup>-2 </sup>respectively. The optimum conditions for the extraction and determination of palladium have been established by monitoring the various experimental parameters. The precision of the method has been evaluated and the relative standard deviation has been found to be less than 0.53%. The proposed method is free from interference from large number of foreign ions. The method has been successfully applied for the determination of palladium from alloy, synthetic mixtures corresponding to alloy samples. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=solvent%20extraction" title="solvent extraction">solvent extraction</a>, <a href="https://publications.waset.org/abstracts/search?q=PMPT" title=" PMPT"> PMPT</a>, <a href="https://publications.waset.org/abstracts/search?q=Palladium%20%28II%29" title=" Palladium (II)"> Palladium (II)</a>, <a href="https://publications.waset.org/abstracts/search?q=spectrophotometry" title=" spectrophotometry"> spectrophotometry</a> </p> <a href="https://publications.waset.org/abstracts/68759/solvent-extraction-and-spectrophotometric-determination-of-palladiumii-using-p-methylphenyl-thiourea-as-a-complexing-agent" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/68759.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">461</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">27929</span> An Investigation of the Use of Visible Spectrophotometric Analysis of Lead in an Herbal Tea Supplement</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Salve%20Alessandria%20Alcantara">Salve Alessandria Alcantara</a>, <a href="https://publications.waset.org/abstracts/search?q=John%20Armand%20E.%20Aquino"> John Armand E. Aquino</a>, <a href="https://publications.waset.org/abstracts/search?q=Ma.%20Veronica%20Aranda"> Ma. Veronica Aranda</a>, <a href="https://publications.waset.org/abstracts/search?q=Nikki%20Francine%20Balde"> Nikki Francine Balde</a>, <a href="https://publications.waset.org/abstracts/search?q=Angeli%20Therese%20F.%20Cruz"> Angeli Therese F. Cruz</a>, <a href="https://publications.waset.org/abstracts/search?q=Elise%20Danielle%20Garcia"> Elise Danielle Garcia</a>, <a href="https://publications.waset.org/abstracts/search?q=Antonie%20Kyna%20Lim"> Antonie Kyna Lim</a>, <a href="https://publications.waset.org/abstracts/search?q=Divina%20Gracia%20Lucero"> Divina Gracia Lucero</a>, <a href="https://publications.waset.org/abstracts/search?q=Nikolai%20Thadeus%20Mappatao"> Nikolai Thadeus Mappatao</a>, <a href="https://publications.waset.org/abstracts/search?q=Maylan%20N.%20Ocat"> Maylan N. Ocat</a>, <a href="https://publications.waset.org/abstracts/search?q=Jamille%20Dyanne%20L.%20Pajarillo"> Jamille Dyanne L. Pajarillo</a>, <a href="https://publications.waset.org/abstracts/search?q=Jane%20Mierial%20A.%20Pesigan"> Jane Mierial A. Pesigan</a>, <a href="https://publications.waset.org/abstracts/search?q=Grace%20Kristin%20Viva"> Grace Kristin Viva</a>, <a href="https://publications.waset.org/abstracts/search?q=Jasmine%20Arielle%20C.%20Yap"> Jasmine Arielle C. Yap</a>, <a href="https://publications.waset.org/abstracts/search?q=Kathleen%20Michelle%20T.%20Yu"> Kathleen Michelle T. Yu</a>, <a href="https://publications.waset.org/abstracts/search?q=Joanna%20J.%20Orejola"> Joanna J. Orejola</a>, <a href="https://publications.waset.org/abstracts/search?q=Joanna%20V.%20Toralba"> Joanna V. Toralba </a> </p> <p class="card-text"><strong>Abstract:</strong></p> Lead is a neurotoxic metallic element that is slowly accumulated in bones and tissues especially if present in products taken in a regular basis such as herbal tea supplements. Although sensitive analytical instruments are already available, the USP limit test for lead is still widely used. However, because of its serious shortcomings, Lang Lang and his colleagues developed a spectrophotometric method for determination of lead in all types of samples. This method was the one adapted in this study. The actual procedure performed was divided into three parts: digestion, extraction and analysis. For digestion, HNO3 and CH3COOH were used. Afterwards, masking agents, 0.003% and 0.001% dithizone in CHCl3 were added and used for the extraction. For the analysis, standard addition method and colorimetry were performed. This was done in triplicates under two conditions. The 1st condition, using 25µg/mL of standard, resulted to very low absorbances with an r2 of 0.551. This led to the use of a higher concentration, 1mg/mL, for condition 2. Precipitation of lead cyanide was observed and the absorbance readings were relatively higher but between 0.15-0.25, resulting to a very low r2 of 0.429. LOQ and LOD were not computed due to the limitations of the Milton-Roy Spectrophotometer. The method performed has a shorter digestion time, and used less but more accessible reagents. However, the optimum ratio of dithizone-lead complex must be observed in order to obtain reliable results while exploring other concentration of standards. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=herbal%20tea%20supplement" title="herbal tea supplement">herbal tea supplement</a>, <a href="https://publications.waset.org/abstracts/search?q=lead-dithizone%20complex" title=" lead-dithizone complex"> lead-dithizone complex</a>, <a href="https://publications.waset.org/abstracts/search?q=standard%20addition" title=" standard addition"> standard addition</a>, <a href="https://publications.waset.org/abstracts/search?q=visible%20spectroscopy" title=" visible spectroscopy"> visible spectroscopy</a> </p> <a href="https://publications.waset.org/abstracts/10463/an-investigation-of-the-use-of-visible-spectrophotometric-analysis-of-lead-in-an-herbal-tea-supplement" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/10463.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">387</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">27928</span> Spectrophotometric Determination of Photohydroxylated Products of Humic Acid in the Presence of Salicylate Probe</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Julide%20Hizal%20Yucesoy">Julide Hizal Yucesoy</a>, <a href="https://publications.waset.org/abstracts/search?q=Batuhan%20Yardimci"> Batuhan Yardimci</a>, <a href="https://publications.waset.org/abstracts/search?q=Aysem%20Arda"> Aysem Arda</a>, <a href="https://publications.waset.org/abstracts/search?q=Resat%20Apak"> Resat Apak</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Humic substances produce reactive oxygene species such as hydroxyl, phenoxy and superoxide radicals by oxidizing in a wide pH and reduction potential range. Hydroxyl radicals, produced by reducing agents such as antioxidants and/or peroxides, attack on salicylate probe, and form 2,3-dihydroxybenzoate, 2,4-dihydroxybenzoate and 2,5-dihydroxybenzoate species. These species are quantitatively determined by using HPLC Method. Humic substances undergo photodegradation by UV radiation. As a result of their antioxidant properties, they produce hydroxyl radicals. In the presence of salicylate probe, these hydroxyl radicals react with salicylate molecules to form hydroxylated products (dihidroxybenzoate isomers). In this study, humic acid was photodegraded in a photoreactor at 254 nm (400W), formed hydroxyl radicals were caught by salicylate probe. The total concentration of hydroxylated salicylate species was measured by using spectrophotometric CUPRAC Method. And also, using results of time dependent experiments, kinetic of photohydroxylation was determined at different pHs. This method has been applied for the first time to measure the concentration of hydroxylated products. It allows to achieve the results easier than HPLC Method. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=CUPRAC%20method" title="CUPRAC method">CUPRAC method</a>, <a href="https://publications.waset.org/abstracts/search?q=humic%20acid" title=" humic acid"> humic acid</a>, <a href="https://publications.waset.org/abstracts/search?q=photohydroxylation" title=" photohydroxylation"> photohydroxylation</a>, <a href="https://publications.waset.org/abstracts/search?q=salicylate%20probe" title=" salicylate probe"> salicylate probe</a> </p> <a href="https://publications.waset.org/abstracts/68393/spectrophotometric-determination-of-photohydroxylated-products-of-humic-acid-in-the-presence-of-salicylate-probe" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/68393.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">206</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">27927</span> Spectrophotometric Detection of Histidine Using Enzyme Reaction and Examination of Reaction Conditions</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Akimitsu%20Kugimiya">Akimitsu Kugimiya</a>, <a href="https://publications.waset.org/abstracts/search?q=Kouhei%20Iwato"> Kouhei Iwato</a>, <a href="https://publications.waset.org/abstracts/search?q=Toru%20Saito"> Toru Saito</a>, <a href="https://publications.waset.org/abstracts/search?q=Jiro%20Kohda"> Jiro Kohda</a>, <a href="https://publications.waset.org/abstracts/search?q=Yasuhisa%20Nakano"> Yasuhisa Nakano</a>, <a href="https://publications.waset.org/abstracts/search?q=Yu%20Takano"> Yu Takano</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The measurement of amino acid content is reported to be useful for the diagnosis of several types of diseases, including lung cancer, gastric cancer, colorectal cancer, breast cancer, prostate cancer, and diabetes. The conventional detection methods for amino acid are high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS), but they have several drawbacks as the equipment is cumbersome and the techniques are costly in terms of time and costs. In contrast, biosensors and biosensing methods provide more rapid and facile detection strategies that use simple equipment. The authors have reported a novel approach for the detection of each amino acid that involved the use of aminoacyl-tRNA synthetase (aaRS) as a molecular recognition element because aaRS is expected to a selective binding ability for corresponding amino acid. The consecutive enzymatic reactions used in this study are as follows: aaRS binds to its cognate amino acid and releases inorganic pyrophosphate. Hydrogen peroxide (H₂O₂) was produced by the enzyme reactions of inorganic pyrophosphatase and pyruvate oxidase. The Trinder’s reagent was added into the reaction mixture, and the absorbance change at 556 nm was measured using a microplate reader. In this study, an amino acid-sensing method using histidyl-tRNA synthetase (HisRS; histidine-specific aaRS) as molecular recognition element in combination with the Trinder’s reagent spectrophotometric method was developed. The quantitative performance and selectivity of the method were evaluated, and the optimal enzyme reaction and detection conditions were determined. The authors developed a simple and rapid method for detecting histidine with a combination of enzymatic reaction and spectrophotometric detection. In this study, HisRS was used to detect histidine, and the reaction and detection conditions were optimized for quantitation of these amino acids in the ranges of 1–100 µM histidine. The detection limits are sufficient to analyze these amino acids in biological fluids. This work was partly supported by Hiroshima City University Grant for Special Academic Research (General Studies). <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=amino%20acid" title="amino acid">amino acid</a>, <a href="https://publications.waset.org/abstracts/search?q=aminoacyl-tRNA%20synthetase" title=" aminoacyl-tRNA synthetase"> aminoacyl-tRNA synthetase</a>, <a href="https://publications.waset.org/abstracts/search?q=biosensing" title=" biosensing"> biosensing</a>, <a href="https://publications.waset.org/abstracts/search?q=enzyme%20reaction" title=" enzyme reaction"> enzyme reaction</a> </p> <a href="https://publications.waset.org/abstracts/70824/spectrophotometric-detection-of-histidine-using-enzyme-reaction-and-examination-of-reaction-conditions" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/70824.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">285</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">27926</span> Development and Validation of First Derivative Method and Artificial Neural Network for Simultaneous Spectrophotometric Determination of Two Closely Related Antioxidant Nutraceuticals in Their Binary Mixture”</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mohamed%20Korany">Mohamed Korany</a>, <a href="https://publications.waset.org/abstracts/search?q=Azza%20Gazy"> Azza Gazy</a>, <a href="https://publications.waset.org/abstracts/search?q=Essam%20Khamis"> Essam Khamis</a>, <a href="https://publications.waset.org/abstracts/search?q=Marwa%20Adel"> Marwa Adel</a>, <a href="https://publications.waset.org/abstracts/search?q=Miranda%20Fawzy"> Miranda Fawzy</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: Two new, simple and specific methods; First, a Zero-crossing first-derivative technique and second, a chemometric-assisted spectrophotometric artificial neural network (ANN) were developed and validated in accordance with ICH guidelines. Both methods were used for the simultaneous estimation of the two closely related antioxidant nutraceuticals ; Coenzyme Q10 (Q) ; also known as Ubidecarenone or Ubiquinone-10, and Vitamin E (E); alpha-tocopherol acetate, in their pharmaceutical binary mixture. Results: For first method: By applying the first derivative, both Q and E were alternatively determined; each at the zero-crossing of the other. The D1 amplitudes of Q and E, at 285 nm and 235 nm respectively, were recorded and correlated to their concentrations. The calibration curve is linear over the concentration range of 10-60 and 5.6-70 μg mL-1 for Q and E, respectively. For second method: ANN (as a multivariate calibration method) was developed and applied for the simultaneous determination of both analytes. A training set (or a concentration set) of 90 different synthetic mixtures containing Q and E, in wide concentration ranges between 0-100 µg/mL and 0-556 µg/mL respectively, were prepared in ethanol. The absorption spectra of the training sets were recorded in the spectral region of 230–300 nm. A Gradient Descend Back Propagation ANN chemometric calibration was computed by relating the concentration sets (x-block) to their corresponding absorption data (y-block). Another set of 45 synthetic mixtures of the two drugs, in defined range, was used to validate the proposed network. Neither chemical separation, preparation stage nor mathematical graphical treatment were required. Conclusions: The proposed methods were successfully applied for the assay of Q and E in laboratory prepared mixtures and combined pharmaceutical tablet with excellent recoveries. The ANN method was superior over the derivative technique as the former determined both drugs in the non-linear experimental conditions. It also offers rapidity, high accuracy, effort and money saving. Moreover, no need for an analyst for its application. Although the ANN technique needed a large training set, it is the method of choice in the routine analysis of Q and E tablet. No interference was observed from common pharmaceutical additives. The results of the two methods were compared together <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=coenzyme%20Q10" title="coenzyme Q10">coenzyme Q10</a>, <a href="https://publications.waset.org/abstracts/search?q=vitamin%20E" title=" vitamin E"> vitamin E</a>, <a href="https://publications.waset.org/abstracts/search?q=chemometry" title=" chemometry"> chemometry</a>, <a href="https://publications.waset.org/abstracts/search?q=quantitative%20analysis" title=" quantitative analysis"> quantitative analysis</a>, <a href="https://publications.waset.org/abstracts/search?q=first%20derivative%20spectrophotometry" title=" first derivative spectrophotometry"> first derivative spectrophotometry</a>, <a href="https://publications.waset.org/abstracts/search?q=artificial%20neural%20network" title=" artificial neural network "> artificial neural network </a> </p> <a href="https://publications.waset.org/abstracts/20123/development-and-validation-of-first-derivative-method-and-artificial-neural-network-for-simultaneous-spectrophotometric-determination-of-two-closely-related-antioxidant-nutraceuticals-in-their-binary-mixture" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/20123.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">446</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">27925</span> Evaluation of the Phenolic Composition of Curcumin from Different Turmeric (Curcuma longa L.) Extracts: A Comprehensive Study Based on Chemical Turmeric Extract, Turmeric Tea and Fresh Turmeric Juice</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Beyza%20Sukran%20Isik">Beyza Sukran Isik</a>, <a href="https://publications.waset.org/abstracts/search?q=Gokce%20Altin"> Gokce Altin</a>, <a href="https://publications.waset.org/abstracts/search?q=Ipek%20Yalcinkaya"> Ipek Yalcinkaya</a>, <a href="https://publications.waset.org/abstracts/search?q=Evren%20Demircan"> Evren Demircan</a>, <a href="https://publications.waset.org/abstracts/search?q=Asli%20Can%20Karaca"> Asli Can Karaca</a>, <a href="https://publications.waset.org/abstracts/search?q=Beraat%20Ozcelik"> Beraat Ozcelik</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Turmeric (Curcuma longa L.), is used as a food additive (spice), preservative and coloring agent in Asian countries, including China and South East Asia. It is also considered as a medicinal plant. Traditional Indian medicine evaluates turmeric powder for the treatment of biliary disorders, rheumatism, and sinusitis. It has rich polyphenol content. Turmeric has yellow color mainly because of the presence of three major pigments; curcumin 1,7-bis(4-hydroxy-3-methoxyphenyl)-1, 6-heptadiene-3,5-dione), demethoxy-curcumin and bis demothoxy-curcumin. These curcuminoids are recognized to have high antioxidant activities. Curcumin is the major constituent of Curcuma species. Method: To prepare turmeric tea, 0.5 gram of turmeric powder was brewed with 250 ml of water at 90°C, 10 minutes. 500 grams of fresh turmeric washed and shelled prior to squeezing. Both turmeric tea and turmeric juice pass through 45 lm filters and stored at -20°C in the dark for further analyses. Curcumin was extracted from 20 grams of turmeric powder by 70 ml ethanol solution (95:5 ethanol/water v/v) in a water bath at 80°C, 6 hours. Extraction was contributed for 2 hours at the end of 6 hours by addition of 30 ml ethanol. Ethanol was removed by rotary evaporator. Remained extract stored at -20°C in the dark. Total phenolic content and phenolic profile were determined by spectrophotometric analysis and ultra-fast liquid chromatography (UFLC), respectively. Results: The total phenolic content of ethanolic extract of turmeric, turmeric juice, and turmeric tea were determined 50.72, 31.76 and 29.68 ppt, respectively. The ethanolic extract of turmeric, turmeric juice, and turmeric tea have been injected into UFLC and analyzed for curcumin contents. The curcumin content in ethanolic extract of turmeric, turmeric juice, and turmeric tea were 4067.4, 156.7 ppm and 1.1 ppm, respectively. Significance: Turmeric is known as a good source of curcumin. According to the results, it can be stated that its tea is not sufficient way for curcumin consumption. Turmeric juice can be preferred to turmeric tea for higher curcumin content. Ethanolic extract of turmeric showed the highest content of turmeric in both spectrophotometric and chromatographic analyses. Nonpolar solvents and carriers which have polar binding sites have to be considered for curcumin consumption due to its nonpolar nature. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=phenolic%20compounds" title="phenolic compounds">phenolic compounds</a>, <a href="https://publications.waset.org/abstracts/search?q=spectrophotometry" title=" spectrophotometry"> spectrophotometry</a>, <a href="https://publications.waset.org/abstracts/search?q=turmeric" title=" turmeric"> turmeric</a>, <a href="https://publications.waset.org/abstracts/search?q=UFLC" title=" UFLC"> UFLC</a> </p> <a href="https://publications.waset.org/abstracts/89625/evaluation-of-the-phenolic-composition-of-curcumin-from-different-turmeric-curcuma-longa-l-extracts-a-comprehensive-study-based-on-chemical-turmeric-extract-turmeric-tea-and-fresh-turmeric-juice" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/89625.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">200</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">27924</span> Determination of Cyanotoxins from Leeukraal and Klipvoor Dams</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Moletsane%20Makgotso">Moletsane Makgotso</a>, <a href="https://publications.waset.org/abstracts/search?q=Mogakabe%20Elijah"> Mogakabe Elijah</a>, <a href="https://publications.waset.org/abstracts/search?q=Marrengane%20Zinhle"> Marrengane Zinhle</a> </p> <p class="card-text"><strong>Abstract:</strong></p> South Africa’s water resources quality is becoming more and more weakened by eutrophication, which deteriorates its usability. Thirty five percent of fresh water resources are eutrophic to hypertrophic, including grossly-enriched reservoirs that go beyond the globally-accepted definition of hypertrophy. Failing infrastructure adds to the problem of contaminated urban runoff which encompasses an important fraction of flows to inland reservoirs, particularly in the non-coastal, economic heartland of the country. Eutrophication threatens the provision of potable and irrigation water in the country because of the dependence on fresh water resources. Eutrophicated water reservoirs increase water treatment costs, leads to unsuitability for recreational purposes and health risks to human and animal livelihood due to algal proliferation. Eutrophication is caused by high concentrations of phosphorus and nitrogen in water bodies. In South Africa, Microsystis and Anabaena are widely distributed cyanobacteria, with Microcystis being the most dominant bloom-forming cyanobacterial species associated with toxin production. Two impoundments were selected, namely the Klipvoor and Leeukraal dams as they are mainly used for fishing, recreational, agricultural and to some extent, potable water purposes. The total oxidized nitrogen and total phosphorus concentration were determined as causative nutrients for eutrophication. Chlorophyll a and total microcystins, as well as the identification of cyanobacteria was conducted as indicators of cyanobacterial infestation. The orthophosphate concentration was determined by subjecting the samples to digestion and filtration followed by spectrophotometric analysis of total phosphates and dissolved phosphates using Aquakem kits. The total oxidized nitrates analysis was conducted by initially conducting filtration followed by spectrophotometric analysis. Chlorophyll a was quantified spectrophotometrically by measuring the absorbance of before and after acidification. Microcystins were detected using the Quantiplate Microcystin Kit, as well as microscopic identification of cyanobacterial species. The Klipvoor dam was found to be hypertrophic throughout the study period as the mean Chlorophyll a concentration was 269.4µg/l which exceeds the mean value for the hypertrophic state. The mean Total Phosphorus concentration was >0.130mg/l, and the total microcystin concentration was > 2.5µg/l throughout the study. The most predominant algal species were found to be the Microcystis. The Leeukraal dam was found to be mesotrophic with the potential of it becoming eutrophic as the mean concentration for chlorophyll a was 18.49 µg/l with the mean Total Phosphorus > 0.130mg/l and the Total Microcystin concentration < 0.16µg/l. The cyanobacterial species identified in Leeukraal have been classified as those that do not pose a potential risk to any impoundment. Microcystis was present throughout the sampling period and dominant during the warmer seasons. The high nutrient concentrations led to the dominance of Microcystis that resulted in high levels of microcystins rendering the impoundments, particularly Klipvoor undesirable for utilisation. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=nitrogen" title="nitrogen">nitrogen</a>, <a href="https://publications.waset.org/abstracts/search?q=phosphorus" title=" phosphorus"> phosphorus</a>, <a href="https://publications.waset.org/abstracts/search?q=cyanobacteria" title=" cyanobacteria"> cyanobacteria</a>, <a href="https://publications.waset.org/abstracts/search?q=microcystins" title=" microcystins"> microcystins</a> </p> <a href="https://publications.waset.org/abstracts/37531/determination-of-cyanotoxins-from-leeukraal-and-klipvoor-dams" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/37531.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">287</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">27923</span> Concentrations and History of Heavy Metals in Sediment Cores: Geochemistry and Geochronology Using 210Pb</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=F.%20Fernandes">F. Fernandes</a>, <a href="https://publications.waset.org/abstracts/search?q=C.%20Poleto"> C. Poleto</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This paper aims at assessing the concentrations of heavy metals and the isotopic composition of lead <sup>210</sup>Pb in different fractions of sediment produced in the watershed that makes up the Mãe d'água dam and thus characterizing the distribution of metals along the sedimentary column and inferencing in the urbanization of the same process. Sample collection was carried out in June 2014; eight sediment cores were sampled in the lake of the dam. For extraction of the sediments core, a core sampler “Piston Core” was used. The trace metal concentrations were determined by conventional atomic absorption spectrophotometric methods. The samples were subjected to radiochemical analysis of <sup>210</sup>Po. <sup>210</sup>Pb activity was obtained by measuring <sup>210</sup>Po activity. The chronology was calculated using the constant rate of supply (CRS). <sup>210</sup>Pb is used to estimate the sedimentation rate. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=%C2%B2%C2%B9%E2%81%B0Pb%20dating%20method" title="²¹⁰Pb dating method">²¹⁰Pb dating method</a>, <a href="https://publications.waset.org/abstracts/search?q=heavy%20metal" title=" heavy metal"> heavy metal</a>, <a href="https://publications.waset.org/abstracts/search?q=lakes%20urban" title=" lakes urban"> lakes urban</a>, <a href="https://publications.waset.org/abstracts/search?q=pollution%20history" title=" pollution history"> pollution history</a> </p> <a href="https://publications.waset.org/abstracts/47912/concentrations-and-history-of-heavy-metals-in-sediment-cores-geochemistry-and-geochronology-using-210pb" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/47912.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">298</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">27922</span> Development of Polymeric Fluorescence Sensor for the Determination of Bisphenol-A</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ne%C5%9Fe%20Ta%C5%9Fci">Neşe Taşci</a>, <a href="https://publications.waset.org/abstracts/search?q=Soner%20%C3%87ubuk"> Soner Çubuk</a>, <a href="https://publications.waset.org/abstracts/search?q=Ece%20K%C3%B6k%20Yetimo%C4%9Flu"> Ece Kök Yetimoğlu</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Vezir%20Kahraman"> M. Vezir Kahraman</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Bisphenol-A (BPA), 2,2-bis(4-hydroxyphenly)propane, is one of the highest usage volume chemicals in the world. Studies showed that BPA maybe has negative effects on the central nervous system, immune and endocrine systems. Several of analytical methods for the analysis of BPA have been reported including electrochemical processes, chemical oxidation, ozonization, spectrophotometric, chromatographic techniques. Compared with other conventional analytical techniques, optic sensors are reliable, providing quick results, low cost, easy to use, stands out as a much more advantageous method because of the high precision and sensitivity. In this work, a new photocured polymeric fluorescence sensor was prepared and characterized for Bisphenol-A (BPA) analysis. Characterization of the membrane was carried out by Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR) and Scanning Electron Microscope (SEM) techniques. The response characteristics of the sensor including dynamic range, pH effect and response time were systematically investigated. Acknowledgment: This work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK) under Grant 115Y469. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bisphenol-a" title="bisphenol-a">bisphenol-a</a>, <a href="https://publications.waset.org/abstracts/search?q=fluorescence" title=" fluorescence"> fluorescence</a>, <a href="https://publications.waset.org/abstracts/search?q=photopolymerization" title=" photopolymerization"> photopolymerization</a>, <a href="https://publications.waset.org/abstracts/search?q=polymeric%20sensor" title=" polymeric sensor"> polymeric sensor</a> </p> <a href="https://publications.waset.org/abstracts/48389/development-of-polymeric-fluorescence-sensor-for-the-determination-of-bisphenol-a" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/48389.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">236</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">27921</span> Investigation of the Possible Beneficial and Protective Effects of an Ethanolic Extract from Sarcopoterium spinosum Fruits</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Hawraa%20Zbeeb">Hawraa Zbeeb</a>, <a href="https://publications.waset.org/abstracts/search?q=Hala%20Khalifeh"> Hala Khalifeh</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohamad%20Khalil"> Mohamad Khalil</a>, <a href="https://publications.waset.org/abstracts/search?q=Francesca%20Storace"> Francesca Storace</a>, <a href="https://publications.waset.org/abstracts/search?q=Francesca%20Baldini"> Francesca Baldini</a>, <a href="https://publications.waset.org/abstracts/search?q=Giulio%20Lupidi"> Giulio Lupidi</a>, <a href="https://publications.waset.org/abstracts/search?q=Laura%20Vergani"> Laura Vergani</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Sarcopoterium spinosum, a widely distributed spiny shrub belonging to the Rosaceae family, is rich in essential and beneficial constituents. In fact, S. spinosum fruits and roots are traditionally used as herbal medicine in the eastern Mediterranean landscape, and this shrub is mentioned as a medicinal plant in a large number of ethnobotanical surveys. Aqueous root extracts from S. spinosum are used by traditional medicinal practitioners for weight loss treatment of diabetes and pain. Moreover, the anti-diabetic activity of S. spinosum root extract has been reported in different studies, but the beneficial effects of aerial parts, especially fruits, have not been elucidated yet. The aim of the present study was to investigate the in vitro antioxidant and lipid-lowering properties of an ethanolic extract from S. spinosum fruits using both hepatic (FaO) and endothelial (HECV) cells in an attempt to evaluate its possible employment as a nutraceutical supplement. First of all, in vitro spectrophotometric assays were employed to characterize the extract. The total phenol content (TPC) was evaluated by Folin–Ciocalteu spectrophotometric method and the radical scavenging activity was tested by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2, 2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assays. After that, the beneficial effects of the extract were tested on cells. FaO cells treated for 3 hours with 0.75 mM oleate/palmitate mix (1:2 molar ratio) mimic in vitro a moderate hepato-steatosis. HECV cells exposed for 1 hour to 100 µM H₂O₂ mimic an oxidative insult leading to oxidative stress conditions. After the metabolic and oxidative insult, both cell lines were treated with increasing concentrations of the S. spinosum extract (1, 10, 25 µg/mL) for 24 hours. The results showed the S. spinosum ethanolic extract is rather rich in phenols (TPC of 18.6 mgGAE/g dry extracts). Moreover, the extract showed a good scavenging ability in vitro (IC₅₀ 15.9 µg/ml and 10.9 µg/ml measured by DPPH and ABTS assays, respectively). When the extract was tested on cells, the results showed that it could ameliorate some markers of cell dysfunction. The three concentrations of the extract led to a significant decrease in the intracellular triglyceride (TG) content in steatotic FaO cells measured by spectrophotometric assay. On the other hand, HECV cells treated with increasing concentrations of the extract did not result in a significant decrease in both lipid peroxidation measured by the Thiobarbituric Acid Reactive Substances (TBARS) assay, and in reactive oxygen species (ROS) production measured by fluorometric analysis after DCF staining. Interestingly, the ethanolic extract was able to accelerate the wound repair of confluent HECV cells with respect to H₂O₂-insulted cells as measured by T-scratch assay. Taken together, these results seem to indicate that the ethanol extract from S. spinosum fruits is rich in phenol compounds and plays considerable lipid-lowering activity in vitro on steatotic hepatocytes and accelerates wound healing repair on endothelial cells. In light of that, the ethanolic extract from S. spinosum fruits could be a potential candidate for nutraceutical applications. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=antioxidant%20activity" title="antioxidant activity">antioxidant activity</a>, <a href="https://publications.waset.org/abstracts/search?q=ethanolic%20extract" title=" ethanolic extract"> ethanolic extract</a>, <a href="https://publications.waset.org/abstracts/search?q=lipid-lowering%20activity" title=" lipid-lowering activity"> lipid-lowering activity</a>, <a href="https://publications.waset.org/abstracts/search?q=phenolic%20compounds" title=" phenolic compounds"> phenolic compounds</a>, <a href="https://publications.waset.org/abstracts/search?q=Sarcopoterium%20spinosum%20fruits" title=" Sarcopoterium spinosum fruits"> Sarcopoterium spinosum fruits</a> </p> <a href="https://publications.waset.org/abstracts/143381/investigation-of-the-possible-beneficial-and-protective-effects-of-an-ethanolic-extract-from-sarcopoterium-spinosum-fruits" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/143381.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">175</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">27920</span> Different Biological and Chemical Parameters that Influence the Polyphenols from Some Medicinal Plants in Western Algeria</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mustapha%20Mahmoud">Mustapha Mahmoud</a>, <a href="https://publications.waset.org/abstracts/search?q=Fouzia%20Toumi%20Benali"> Fouzia Toumi Benali</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohamed%20Benyahia"> Mohamed Benyahia</a>, <a href="https://publications.waset.org/abstracts/search?q=Sofiane%20Bouazza"> Sofiane Bouazza </a> </p> <p class="card-text"><strong>Abstract:</strong></p> This work focuses on the influences of biological and chemical parameters on the phenolic compounds such as flavonoids and tannins in different medicinal plants in western Algeria (Papaver rhoeas, Daphnegnidium, Lavandula multifida, Lavandula dentata, Lavandula stoicha, ...). Thus we look the difference between species of the same genus, difference between the different organs of the same species, the influence of environment all temperature influences, time, percentage of solvent on the extraction. Quantification of the phenolic compounds was performed by spectrophotometric method then treated with statistics tools such as variance analysis, multivariant analyzes, response surface methodology). The results show that the polyphenols are influenced by the parameters mentioned. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=polyphenols" title="polyphenols">polyphenols</a>, <a href="https://publications.waset.org/abstracts/search?q=influences" title=" influences"> influences</a>, <a href="https://publications.waset.org/abstracts/search?q=medicinal%20plants" title=" medicinal plants"> medicinal plants</a>, <a href="https://publications.waset.org/abstracts/search?q=west%20Algeria" title=" west Algeria"> west Algeria</a> </p> <a href="https://publications.waset.org/abstracts/56767/different-biological-and-chemical-parameters-that-influence-the-polyphenols-from-some-medicinal-plants-in-western-algeria" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/56767.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">294</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">27919</span> Bioconcentration Analysis of Iodine Species in Seaweed (Eucheuma cottonii) from Maluku Marine as Alternative Food Source</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Yeanchon%20H.%20Dulanlebit">Yeanchon H. Dulanlebit</a>, <a href="https://publications.waset.org/abstracts/search?q=Nikmans%20Hattu"> Nikmans Hattu</a>, <a href="https://publications.waset.org/abstracts/search?q=Gloria%20Bora"> Gloria Bora</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Seaweed is a type of macro algae which are good source of iodine and have been widely used as food and nutrition supplement. One of iodine species that found in ocean plant is iodate. Analysis of iodate in seaweed (<em>Eucheuma cottonii</em>) from coastal area of Maluku has been done. The determination is done by using spectrophotometric method. Iodate in sample is reduced in excess of potassium iodide in the presence of acid solution, and then is reacted with starch to form blue complex. The study found out that the highest wavelength on determination of iodate species using spectrophotometer analysis method is 570 nm. Optimum value to yield maximum absorption is used in this research. Contents of iodate in seawater from coastal area of Ambon Island, Western Seram and Southeast Maluku are 0.2655, 0.2719 and 0.1760 mg/L, respectively. While in seaweeds from Ambon Island, Western Seram, Southeast Maluku-Taar, Ohoidertawun and Wab are 6.3122, 6.3293, 6.2333, 3.7406 and 4.4207 mg/kg in dry weight. Bioconcentration (enrichment) factor of iodate in seaweed (<em>Eucheuma cottonii</em>) from the three samples (cluster) is different; in Coastal area of Ambon Island, Western Seram and Southeast Maluku respectively are 23.78, 23.28 and 27.26. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bioconcentration" title="bioconcentration">bioconcentration</a>, <a href="https://publications.waset.org/abstracts/search?q=eucheuma%20cottonii" title=" eucheuma cottonii"> eucheuma cottonii</a>, <a href="https://publications.waset.org/abstracts/search?q=iodate" title=" iodate"> iodate</a>, <a href="https://publications.waset.org/abstracts/search?q=iodine" title=" iodine"> iodine</a>, <a href="https://publications.waset.org/abstracts/search?q=seaweed" title=" seaweed"> seaweed</a> </p> <a href="https://publications.waset.org/abstracts/75025/bioconcentration-analysis-of-iodine-species-in-seaweed-eucheuma-cottonii-from-maluku-marine-as-alternative-food-source" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/75025.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">219</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">27918</span> Synthesis and Spectrophotometric Study of Omeprazole Charge Transfer Complexes with Bromothymol Blue, Methyl Orange, and Picric Acid</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Saeeda%20Nadir%20Ali">Saeeda Nadir Ali</a>, <a href="https://publications.waset.org/abstracts/search?q=Najma%20Sultana"> Najma Sultana</a>, <a href="https://publications.waset.org/abstracts/search?q=Muhammad%20Saeed%20Arayne"> Muhammad Saeed Arayne</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Charge transfer complexes of omeprazole with bromothymol blue, methyl orange, and picric acid in the Beer’s law ranges 7-56, 6-48, and 10-80 µg mL-1, exhibiting stoichiometric ratio 1:1, and maximum wavelength 400, 420 and 373 nm respectively have been studied in aqueous medium. ICH guidelines were followed for validation study. Spectroscopic parameters including oscillator’s strength, dipole moment, ionization potential, energy of complexes, resonance energy, association constant and Gibb’s free energy changes have also been investigated and Benesi-Hildebrand plot in each case has been obtained. In addition, the methods were fruitfully employed for omeprazole determination in pharmaceutical formulations with no excipients obstruction during analysis. Solid omeprazole complexes with all the acceptors were synthesized and then structure was elucidated by IR and 1H NMR spectroscopy. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=omeprazole" title="omeprazole">omeprazole</a>, <a href="https://publications.waset.org/abstracts/search?q=bromothymol%20blue" title=" bromothymol blue"> bromothymol blue</a>, <a href="https://publications.waset.org/abstracts/search?q=methyl%20orange%20and%20picric%20acid" title=" methyl orange and picric acid"> methyl orange and picric acid</a>, <a href="https://publications.waset.org/abstracts/search?q=charge%20transfer%20complexes" title=" charge transfer complexes"> charge transfer complexes</a> </p> <a href="https://publications.waset.org/abstracts/21749/synthesis-and-spectrophotometric-study-of-omeprazole-charge-transfer-complexes-with-bromothymol-blue-methyl-orange-and-picric-acid" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/21749.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">540</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">27917</span> Spectral Analysis Approaches for Simultaneous Determination of Binary Mixtures with Overlapping Spectra: An Application on Pseudoephedrine Sulphate and Loratadine</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sara%20El-Hanboushy">Sara El-Hanboushy</a>, <a href="https://publications.waset.org/abstracts/search?q=Hayam%20Lotfy"> Hayam Lotfy</a>, <a href="https://publications.waset.org/abstracts/search?q=Yasmin%20Fayez"> Yasmin Fayez</a>, <a href="https://publications.waset.org/abstracts/search?q=Engy%20Shokry"> Engy Shokry</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohammed%20%20Abdelkawy"> Mohammed Abdelkawy</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Simple, speciﬁc, accurate and precise spectrophotometric methods are developed and validated for simultaneous determination of pseudoephedrine sulphate (PSE) and loratadine (LOR) in combined dosage form based on spectral analysis technique. Pseudoephedrine (PSE) in binary mixture could be analyzed either by using its resolved zero order absorption spectrum at its λ max 256.8 nm after subtraction of LOR spectrum or in presence of LOR spectrum by absorption correction method at 256.8 nm, dual wavelength (DWL) method at 254nm and 273nm, induced dual wavelength (IDWL) method at 256nm and 272nm and ratio difference (RD) method at 256nm and 262 nm. Loratadine (LOR) in the mixture could be analyzed directly at 280nm without any interference of PSE spectrum or at 250 nm using its recovered zero order absorption spectrum using constant multiplication(CM).In addition, simultaneous determination for PSE and LOR in their mixture could be applied by induced amplitude modulation method (IAM) coupled with amplitude multiplication (PM). <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=dual%20wavelength%20%28DW%29" title="dual wavelength (DW)">dual wavelength (DW)</a>, <a href="https://publications.waset.org/abstracts/search?q=induced%20amplitude%20modulation%20method%20%28IAM%29%20coupled%20with%20amplitude%20multiplication%20%28PM%29" title=" induced amplitude modulation method (IAM) coupled with amplitude multiplication (PM)"> induced amplitude modulation method (IAM) coupled with amplitude multiplication (PM)</a>, <a href="https://publications.waset.org/abstracts/search?q=loratadine" title=" loratadine"> loratadine</a>, <a href="https://publications.waset.org/abstracts/search?q=pseudoephedrine%20sulphate" title=" pseudoephedrine sulphate"> pseudoephedrine sulphate</a>, <a href="https://publications.waset.org/abstracts/search?q=ratio%20difference%20%28RD%29" title=" ratio difference (RD)"> ratio difference (RD)</a> </p> <a href="https://publications.waset.org/abstracts/53587/spectral-analysis-approaches-for-simultaneous-determination-of-binary-mixtures-with-overlapping-spectra-an-application-on-pseudoephedrine-sulphate-and-loratadine" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/53587.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">321</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">27916</span> A Fluorescent Polymeric Boron Sensor</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Soner%20Cubuk">Soner Cubuk</a>, <a href="https://publications.waset.org/abstracts/search?q=Mirgul%20Kosif"> Mirgul Kosif</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Vezir%20Kahraman"> M. Vezir Kahraman</a>, <a href="https://publications.waset.org/abstracts/search?q=Ece%20Kok%20Yetimoglu"> Ece Kok Yetimoglu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Boron is an essential trace element for the completion of the life circle for organisms. Suitable methods for the determination of boron have been proposed, including acid - base titrimetric, inductively coupled plasma emission spectroscopy flame atomic absorption and spectrophotometric. However, the above methods have some disadvantages such as long analysis times, requirement of corrosive media such as concentrated sulphuric acid and multi-step sample preparation requirements and time-consuming procedures. In this study, a selective and reusable fluorescent sensor for boron based on glycosyloxyethyl methacrylate was prepared by photopolymerization. The response characteristics such as response time, pH, linear range, limit of detection were systematically investigated. The excitation/emission maxima of the membrane were at 378/423 nm, respectively. The approximate response time was measured as 50 sec. In addition, sensor had a very low limit of detection which was 0.3 ppb. The sensor was successfully used for the determination of boron in water samples with satisfactory results. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=boron" title="boron">boron</a>, <a href="https://publications.waset.org/abstracts/search?q=fluorescence" title=" fluorescence"> fluorescence</a>, <a href="https://publications.waset.org/abstracts/search?q=photopolymerization" title=" photopolymerization"> photopolymerization</a>, <a href="https://publications.waset.org/abstracts/search?q=polymeric%20sensor" title=" polymeric sensor"> polymeric sensor</a> </p> <a href="https://publications.waset.org/abstracts/41973/a-fluorescent-polymeric-boron-sensor" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/41973.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">283</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">27915</span> Compensatory Increased Activities of Mitochondrial Respiratory Chain Complexes from Eyes of Glucose-Immersed Zebrafish</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jisun%20Jun">Jisun Jun</a>, <a href="https://publications.waset.org/abstracts/search?q=Eun%20Ko"> Eun Ko</a>, <a href="https://publications.waset.org/abstracts/search?q=Sooim%20Shin"> Sooim Shin</a>, <a href="https://publications.waset.org/abstracts/search?q=Kitae%20Kim"> Kitae Kim</a>, <a href="https://publications.waset.org/abstracts/search?q=Moonsung%20Choi"> Moonsung Choi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Diabetes is a metabolic disease characterized by hyperglycemia, insulin resistant, mitochondrial dysfunction. Diabetes is associated with the development of diabetic retinopathy resulting in worsening vision and eventual blindness. In this study, eyes were enucleated from glucose-immersed zebrafish which is a good animal model to generate diabetes, and then mitochondria were isolated to evaluate activities of mitochondrial electron transfer complexes. Surprisingly, the amount of isolated mitochondria was increased in eyes from glucose-immersed zebrafish compared to those from non-glucose-immerged zebrafish. Spectrophotometric analysis for measuring activities of mitochondrial complex I, II, III, and IV revealed that mitochondria functions was even enhanced in eyes from glucose-immersed zebrafish. These results indicated that 3 days or 7 days glucose-immersion on zebrafish to induce diabetes might contribute metabolic compensatory mechanism to restore their mitochondrial homeostasis on the early stage of diabetes in eyes. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=diabetes" title="diabetes">diabetes</a>, <a href="https://publications.waset.org/abstracts/search?q=glucose%20immersion" title=" glucose immersion"> glucose immersion</a>, <a href="https://publications.waset.org/abstracts/search?q=mitochondrial%20complexes" title=" mitochondrial complexes"> mitochondrial complexes</a>, <a href="https://publications.waset.org/abstracts/search?q=zebrafish" title=" zebrafish"> zebrafish</a> </p> <a href="https://publications.waset.org/abstracts/77334/compensatory-increased-activities-of-mitochondrial-respiratory-chain-complexes-from-eyes-of-glucose-immersed-zebrafish" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/77334.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">204</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">27914</span> Phytochemical Evaluation and In-Vitro Antibacterial Activity of Ethanolic Extracts of Moroccan Lavandula x Intermedia Leaves and Flowers</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jamila%20Fliou">Jamila Fliou</a>, <a href="https://publications.waset.org/abstracts/search?q=Federica%20Spinola"> Federica Spinola</a>, <a href="https://publications.waset.org/abstracts/search?q=Ouassima%20Riffi"> Ouassima Riffi</a>, <a href="https://publications.waset.org/abstracts/search?q=Asmaa%20Zriouel"> Asmaa Zriouel</a>, <a href="https://publications.waset.org/abstracts/search?q=Ali%20Amechrouq"> Ali Amechrouq</a>, <a href="https://publications.waset.org/abstracts/search?q=Luca%20Nalbone"> Luca Nalbone</a>, <a href="https://publications.waset.org/abstracts/search?q=Alessandro%20Giuffrida"> Alessandro Giuffrida</a>, <a href="https://publications.waset.org/abstracts/search?q=Filippo%20Giarratana"> Filippo Giarratana</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This study performed a preliminary evaluation of the phytochemical composition and in vitro antibacterial activity of ethanolic extracts of Lavandula x intermedia leaves and flowers collected in the Fez-Meknes region of Morocco. Phytochemical analyses comprised qualitative colourimetric determinations of alkaloids, anthraquinones, and terpenes and quantitative analysis of total polyphenols, flavonoids, and condensed tannins by UV spectrophotometer. Antibacterial activity was evaluated by determining minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values against different ATCC bacterial strains. The phytochemical analysis showed a high amount of total polyphenols, flavonoids, and tannins in the leaf extract and a higher amount of terpenes based on colourimetric reaction than the flower extract. A positive colourimetric reaction for alkaloids and anthraquinones was detected for both extracts. The antibacterial activity of leaves and flower extract was not different against Gram-positive and Gram-negative strains (p<0.05). The results of the present study suggest the possible use of ethanolic extracts of L. x intermedia collected in the Fez-Meknes region of Morocco as a natural agent against bacterial pathogens. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=antimicrobial%20activity" title="antimicrobial activity">antimicrobial activity</a>, <a href="https://publications.waset.org/abstracts/search?q=Lavandula%20spp." title=" Lavandula spp."> Lavandula spp.</a>, <a href="https://publications.waset.org/abstracts/search?q=lavender" title=" lavender"> lavender</a>, <a href="https://publications.waset.org/abstracts/search?q=lavandin" title=" lavandin"> lavandin</a>, <a href="https://publications.waset.org/abstracts/search?q=UV%20spectrophotometric%20analysis" title=" UV spectrophotometric analysis"> UV spectrophotometric analysis</a> </p> <a href="https://publications.waset.org/abstracts/176476/phytochemical-evaluation-and-in-vitro-antibacterial-activity-of-ethanolic-extracts-of-moroccan-lavandula-x-intermedia-leaves-and-flowers" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/176476.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">68</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">27913</span> Improving Dyeability of Cotton Fabric with Juglans regia L. Natural Dyestuff</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=M.%20Heysem%20Arslan">M. Heysem Arslan</a>, <a href="https://publications.waset.org/abstracts/search?q=Ikilem%20Gocek"> Ikilem Gocek</a>, <a href="https://publications.waset.org/abstracts/search?q=U.%20Kivanc%20Sahin"> U. Kivanc Sahin</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Natural dyestuff, extracted from Juglans Regia L., a kind of walnut, was used to dye 100% cotton gabardine fabric. The main goal of this study was to enhance dyeing process of cotton fabric with Juglans Regia L. dyestuff in terms of color fastness values by designing and developing a mordant application process. Within the context of this study, different mordants such as tannic acid, gallic acid, ascorbic acid, potassium sodium tartrate tetrahydrate, calcium carbonate, iron (II) sulphate heptahydrate, aluminum potassium sulphate dodecahydrate and their combinations were applied in the mordanting processes. Spectrophotometric analysis, color fastness to washing and color fastness to light tests were carried out on the fabric samples. In this study, it was shown that by using the right combination of mordants with a proper application process, it is possible to improve color fastness values of cotton fabric samples dyed with natural dyestuff. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=extraction" title="extraction">extraction</a>, <a href="https://publications.waset.org/abstracts/search?q=Juglans%20Regia%20L." title=" Juglans Regia L."> Juglans Regia L.</a>, <a href="https://publications.waset.org/abstracts/search?q=mordanting%20process" title=" mordanting process"> mordanting process</a>, <a href="https://publications.waset.org/abstracts/search?q=natural%20dyestuff" title=" natural dyestuff"> natural dyestuff</a> </p> <a href="https://publications.waset.org/abstracts/61928/improving-dyeability-of-cotton-fabric-with-juglans-regia-l-natural-dyestuff" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/61928.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">310</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">27912</span> Method Validation for Heavy Metal Determination in Spring Water and Sediments</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Habtamu%20Abdisa">Habtamu Abdisa</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Spring water is particularly valuable due to its high mineral content, which is beneficial for human health. However, anthropogenic activities usually imbalance the natural levels of its composition, which can cause adverse health effects. Regular monitoring of a naturally given environmental resource is of great concern in the world today. The spectrophotometric application is one of the best methods for qualifying and quantifying the mineral contents of environmental water samples. This research was conducted to evaluate the quality of spring water concerning its heavy metal composition. A grab sampling technique was employed to collect representative samples, including duplicates. The samples were then treated with concentrated HNO3 to a pH level below 2 and stored at 4oC. The samples were digested and analyzed for cadmium (Cd), chromium (Cr), manganese (Mn), copper (Cu), iron (Fe), and zinc (Zn) following method validation. Atomic Absorption Spectrometry (AAS) was utilized for the sample analysis. Quality control measures, including blanks, duplicates, and certified reference materials (CRMs), were implemented to ensure the accuracy and precision of the analytical results. Of the metals analyzed in the water samples, Cd and Cr were found to be below the detection limit. However, the concentrations of Mn, Cu, Fe, and Zn ranged from mean values of 0.119-0.227 mg/L, 0.142-0.166 mg/L, 0.183-0.267 mg/L, and 0.074-0.181 mg/L, respectively. Sediment analysis revealed mean concentration ranges of 348.31-429.21 mg/kg, 0.23-0.28 mg/kg, 18.73-22.84 mg/kg, 2.76-3.15 mg/kg, 941.84-1128.56 mg/kg, and 42.39-66.53 mg/kg for Mn, Cd, Cu, Cr, Fe, and Zn, respectively. The study results established that the evaluated spring water and its associated sediment met the regulatory standards and guidelines for heavy metal concentrations. Furthermore, this research can enhance the quality assurance and control processes for environmental sample analysis, ensuring the generation of reliable data. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=method%20validation" title="method validation">method validation</a>, <a href="https://publications.waset.org/abstracts/search?q=heavy%20metal" title=" heavy metal"> heavy metal</a>, <a href="https://publications.waset.org/abstracts/search?q=spring%20water" title=" spring water"> spring water</a>, <a href="https://publications.waset.org/abstracts/search?q=sediment" title=" sediment"> sediment</a>, <a href="https://publications.waset.org/abstracts/search?q=method%20detection%20limit" title=" method detection limit"> method detection limit</a> </p> <a href="https://publications.waset.org/abstracts/173392/method-validation-for-heavy-metal-determination-in-spring-water-and-sediments" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/173392.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">68</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">27911</span> Green Synthesis of Silver Nanoparticles with Aqueous Extract of Moringa oleifera Lam Leaves and Its Ameliorative Effect on Benign Prostatic Hyperplasia in Wistar Rat</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Rotimi%20Larayetana">Rotimi Larayetana</a>, <a href="https://publications.waset.org/abstracts/search?q=Yahaya%20Abdulrazaq"> Yahaya Abdulrazaq</a>, <a href="https://publications.waset.org/abstracts/search?q=Oladunni%20O.%20Falola"> Oladunni O. Falola</a>, <a href="https://publications.waset.org/abstracts/search?q=Abayomi%20Ajayi"> Abayomi Ajayi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The aim of this study was to perform green synthesis of silver nanoparticles (AgNPs) with the aqueous extract of Moringa oleifera Lam (M oleifera) leaves and determine its effects on benign prostatic hyperplasia in Wistar rats. Silver nitrate (AgNO₃) solution was reduced using the aqueous extract of Moringa oleifera Lam leaves, the resultant biogenic AgNPs were characterized by Fourier transformed infrared spectrophotometric, SEM, TEM and X-ray diffraction analysis. Animal experiments involved thirty (30) adult male Wistar rats randomly divided into five groups (A to E; n ₌ 5). Group A received only subcutaneous injection of olive oil daily while the other groups got 3 mg/kg/daily of testosterone propionate (TP) subcutaneously plus 50 mg/kg/daily of AgNPs intraperitoneally (B), 3 mg/kg/daily of TP plus 25 mg/kg/daily of AgNPs (C), 3 mg/kg/daily of TP only (D) and 25 mg/kg/daily of AgNPs only (E). The animals were sacrificed after 14 days, and the prostate gland, liver, and kidney were processed for histological analysis. Phytochemical screening and GC-MS analysis were performed to determine the composition of the M oleifera extract used. Biogenic AgNPs with an average diameter of 23 nm were synthesized. Biogenic AgNPs ameliorated hormone-induced prostate enlargement, and the inhibition of prostatic hypertrophy could be due to the presence of a significant amount of plant fatty acids and phytosterols in the aqueous extract of M oleifera extract. However, the administration of biogenic AgNPs at higher doses impacted negatively on the cytoarchitecture of the liver. Green synthesis of AgNPs with the aqueous extract of Moringa oleifera might be beneficial for the treatment of BPH. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=benign%20prostatic%20hyperplasia" title="benign prostatic hyperplasia">benign prostatic hyperplasia</a>, <a href="https://publications.waset.org/abstracts/search?q=biogenic%20synthesis" title=" biogenic synthesis"> biogenic synthesis</a>, <a href="https://publications.waset.org/abstracts/search?q=Moringa%20oleifera" title=" Moringa oleifera"> Moringa oleifera</a>, <a href="https://publications.waset.org/abstracts/search?q=silver%20nanoparticles" title=" silver nanoparticles"> silver nanoparticles</a>, <a href="https://publications.waset.org/abstracts/search?q=testosterone" title=" testosterone"> testosterone</a> </p> <a 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