Search results for: serodiagnostic

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serodiagnostic</title> <meta name="description" content="Search results for: serodiagnostic"> <meta name="keywords" content="serodiagnostic"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none 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action="https://publications.waset.org/abstracts/search"> <div id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="serodiagnostic"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 3</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: serodiagnostic</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3</span> Profile of Serological Response of Equids Naturally Infected with Burkholderia mallei</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Iahtasham%20Khan">Iahtasham Khan</a>, <a href="https://publications.waset.org/abstracts/search?q=Vania%20Lucia%20De%20Assis%20Santana"> Vania Lucia De Assis Santana</a>, <a href="https://publications.waset.org/abstracts/search?q=Marcilia%20Maria%20Alves%20De%20Souza"> Marcilia Maria Alves De Souza</a>, <a href="https://publications.waset.org/abstracts/search?q=Mabel%20Hanna%20%20Vance%20Harrop"> Mabel Hanna Vance Harrop</a>, <a href="https://publications.waset.org/abstracts/search?q=Fernando%20Leandro%20Dos%20Santos"> Fernando Leandro Dos Santos</a>, <a href="https://publications.waset.org/abstracts/search?q=Cec%C3%ADlia%20Maria%20Souza%20Le%C3%A3o%20E.%20Silva"> Cec铆lia Maria Souza Le茫o E. Silva</a>, <a href="https://publications.waset.org/abstracts/search?q=Pedro%20Paulo%20Silveira"> Pedro Paulo Silveira</a>, <a href="https://publications.waset.org/abstracts/search?q=Marcelo%20Brasil"> Marcelo Brasil</a>, <a href="https://publications.waset.org/abstracts/search?q=Marcus%20Vin%C3%ADcius"> Marcus Vin铆cius</a>, <a href="https://publications.waset.org/abstracts/search?q=H%C3%A9lio%20Cordeiro%20Manso%20Filho"> H茅lio Cordeiro Manso Filho</a>, <a href="https://publications.waset.org/abstracts/search?q=Muhammad%20Younus"> Muhammad Younus</a>, <a href="https://publications.waset.org/abstracts/search?q=Aman%20Ullah%20Khan"> Aman Ullah Khan</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Glanders ranks high on clinical lists in some regions of Brazil as a cause of respiratory and lymphatic disease in equids. Glanders is caused by Burkolderia mallei (B. mallei) Gram-negative bacterium. B. mallei was first biological agent used in World War I in 20th century. The complement fixation test (CFT) is a serodiagnostic tool prescribed by the World Organization for Animal Health (OIE)for the diagnosis of glanders in the international trade of equids. The aim of the present study was to monitor the serological responses in equines naturally infected with B. mallei using the CFT. A total of 574 equids were tested with CFT, 30 days apart in a total of 12 samplings. One hundred thirty-four sera tested negative in all samplings; 192 sera tested positive in one sampling and 125 sera tested positive in two or more samplings. Remaining 123 samples showed uncertain results. Thus, CFT results can vary over a period of time. These variations could be the consequence of the effects of the natural immune response in each animal. The findings of the present study demonstrate difficulties regarding the simultaneous implementation of CFT and test and slaughter policies to eradicate glanders. Another constraint to control this disease is the presence of carrier/transitory CFT-negative animals, which are a potential source of disease in glanders-free areas. Serodiagnostic tests of higher sensitivity and specificity like immunobloat should be implemented to achieve success in the eradication of glanders. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=glanders" title="glanders">glanders</a>, <a href="https://publications.waset.org/abstracts/search?q=equids" title=" equids"> equids</a>, <a href="https://publications.waset.org/abstracts/search?q=horses" title=" horses"> horses</a>, <a href="https://publications.waset.org/abstracts/search?q=immunological" title=" immunological"> immunological</a>, <a href="https://publications.waset.org/abstracts/search?q=mules" title=" mules"> mules</a> </p> <a href="https://publications.waset.org/abstracts/68969/profile-of-serological-response-of-equids-naturally-infected-with-burkholderia-mallei" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/68969.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">415</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2</span> Serological Screening of Cytomegalovirus Infection among Sudanese Patients with Leukemia, Breast and Prostate Cancers at Radiation-Isotope Center in Khartoum</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Abuelquasim.%20M.%20Hassan">Abuelquasim. M. Hassan</a>, <a href="https://publications.waset.org/abstracts/search?q=Namarig%20.S.%20Mohammed"> Namarig .S. Mohammed</a>, <a href="https://publications.waset.org/abstracts/search?q=Samah%20F.%20Mohammed"> Samah F. Mohammed</a>, <a href="https://publications.waset.org/abstracts/search?q=Wafaa.%20A.%20Mohammed"> Wafaa. A. Mohammed</a>, <a href="https://publications.waset.org/abstracts/search?q=Wafaa%20M.%20Edriss"> Wafaa M. Edriss</a>, <a href="https://publications.waset.org/abstracts/search?q=Amel%20A.%20Ahmed"> Amel A. Ahmed</a>, <a href="https://publications.waset.org/abstracts/search?q=Elfadil%20M.%20Abass"> Elfadil M. Abass</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Introduction: Cytomegalovirus (CMV), a common virus, usually causes asymptomatic infections in immunocompetent hosts; however, it may lead to serious complications especially in cancer patients. Objectives: This study was conducted to determine the seroprevalence of human cytomegalovirus (HCMV) among leukemia, breast and prostate cancer patients attending at Radiation Isotope-Center-Khartoum (RICK) from April to August 2016. Material and Methods: A total of 91 subjects were included: 30 leukemic, 22 breast cancer and 29 prostate cancer patients.10 of them were healthy and used as control group, serum samples were collected and tested for CMV IgG & IgM using enzyme-linked immune sorbent assay (ELISA). Result: Of the control group, 9/10 (9.9%) were seropositive for CMV IgG and 1/10 (1.09%) were sero positive for IgM. Also, all cancer groups demonstrated presence of IgG antibody classes as: The percentage of positive results in prostate, breast cancer and leukemia were 35.8 %, 37.2%, and 35.3% respectively. Conclusion: There was no significant correlation between leukemia, breast, prostate and HCMV. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cytomegalovirus" title="cytomegalovirus">cytomegalovirus</a>, <a href="https://publications.waset.org/abstracts/search?q=serodiagnostic" title=" serodiagnostic"> serodiagnostic</a>, <a href="https://publications.waset.org/abstracts/search?q=breast%20cancer" title=" breast cancer"> breast cancer</a>, <a href="https://publications.waset.org/abstracts/search?q=leukemia" title=" leukemia"> leukemia</a> </p> <a href="https://publications.waset.org/abstracts/57018/serological-screening-of-cytomegalovirus-infection-among-sudanese-patients-with-leukemia-breast-and-prostate-cancers-at-radiation-isotope-center-in-khartoum" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/57018.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">384</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1</span> Novel p22-Monoclonal Antibody Based Blocking ELISA for the Detection of African Swine Fever Virus Antibodies in Serum</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ghebremedhin%20Tsegay">Ghebremedhin Tsegay</a>, <a href="https://publications.waset.org/abstracts/search?q=Weldu%20Tesfagaber"> Weldu Tesfagaber</a>, <a href="https://publications.waset.org/abstracts/search?q=Yuanmao%20Zhu"> Yuanmao Zhu</a>, <a href="https://publications.waset.org/abstracts/search?q=Xijun%20He"> Xijun He</a>, <a href="https://publications.waset.org/abstracts/search?q=Wan%20Wang"> Wan Wang</a>, <a href="https://publications.waset.org/abstracts/search?q=Zhenjiang%20Zhang"> Zhenjiang Zhang</a>, <a href="https://publications.waset.org/abstracts/search?q=Encheng%20Sun"> Encheng Sun</a>, <a href="https://publications.waset.org/abstracts/search?q=Jinya%20Zhang"> Jinya Zhang</a>, <a href="https://publications.waset.org/abstracts/search?q=Yuntao%20Guan"> Yuntao Guan</a>, <a href="https://publications.waset.org/abstracts/search?q=Fang%20Li"> Fang Li</a>, <a href="https://publications.waset.org/abstracts/search?q=Renqiang%20Liu"> Renqiang Liu</a>, <a href="https://publications.waset.org/abstracts/search?q=Zhigao%20Bu"> Zhigao Bu</a>, <a href="https://publications.waset.org/abstracts/search?q=Dongming%20Zhao%2A"> Dongming Zhao*</a> </p> <p class="card-text"><strong>Abstract:</strong></p> African swine fever (ASF) is a highly infectious viral disease of pigs, resulting in significant economic loss worldwide. As there is no approved vaccines and treatments, the control of ASF entirely depends on early diagnosis and culling of infected pigs. Thus, highly specific and sensitive diagnostic assays are required for accurate and early diagnosis of ASF virus (ASFV). Currently, only a few recombinant proteins have been tested and validated for use as reagents in ASF diagnostic assays. The most promising ones for ASFV antibody detection were p72, p30, p54, and pp62. So far, three ELISA kits based on these recombinant proteins have been commercialized. Due to the complex nature of the virus and variety forms of the disease, robust serodiagnostic assays are still required. ASFV p22 protein, encoded by KP177R gene, is located in the inner membrane of viral particle and appeared transiently in the plasma membrane early after virus infection. The p22 protein interacts with numerous cellular proteins, involved in processes of phagocytosis and endocytosis through different cellular pathways. However, p22 does not seem to be involved in virus replication or swine pathogenicity. In this study, E.coli expressed recombinant p22 protein was used to generate a monoclonal antibody (mAb), and its potential use for the development of blocking ELISA (bELISA) was evaluated. A total of 806 pig serum samples were tested to evaluate the bELISA. Acording the ROC (Reciever operating chracteristic) analysis, 100% sensitivity and 98.10% of specificity was recorded when the PI cut-off value was set at 47%. The novel assay was able to detect the antibodies as early as 9 days post infection. Finaly, a highly sensitive, specific and rapid novel p22-mAb based bELISA assay was developed, and optimized for detection of antibodies against genotype I and II ASFVs. It is a promising candidate for an early and acurate detection of the antibodies and is highly expected to have a valuable role in the containment and prevention of ASF. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=ASFV" title="ASFV">ASFV</a>, <a href="https://publications.waset.org/abstracts/search?q=blocking%20ELISA" title=" blocking ELISA"> blocking ELISA</a>, <a href="https://publications.waset.org/abstracts/search?q=diagnosis" title=" diagnosis"> diagnosis</a>, <a href="https://publications.waset.org/abstracts/search?q=monoclonal%20antibodies" title=" monoclonal antibodies"> monoclonal antibodies</a>, <a href="https://publications.waset.org/abstracts/search?q=sensitivity" title=" sensitivity"> sensitivity</a>, <a href="https://publications.waset.org/abstracts/search?q=specificity" title=" specificity"> specificity</a> </p> <a href="https://publications.waset.org/abstracts/164212/novel-p22-monoclonal-antibody-based-blocking-elisa-for-the-detection-of-african-swine-fever-virus-antibodies-in-serum" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/164212.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">77</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates its 16th foundational 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Search results for: serodiagnostic