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Search results for: hindgut

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paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">5</span> Sulfur-Containing Diet Shift Hydrogen Metabolism and Reduce Methane Emission and Modulated Gut Microbiome in Goats</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Tsegay%20Teklebrhan%20Gebremariam">Tsegay Teklebrhan Gebremariam</a>, <a href="https://publications.waset.org/abstracts/search?q=Zhiliang"> Zhiliang</a>, <a href="https://publications.waset.org/abstracts/search?q=Arjan%20Jonker"> Arjan Jonker</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The study investigated that using corn gluten (CG) instead of cornmeal (CM) increased dietary sulfur shifted H₂ metabolism from methanogenesis to alternative sink and modulated microbiome in the rumen as well as hindgut segments of goats. Ruminal fermentation, CH₄ emissions and microbial abundance in goats (n = 24). The experiment was performed using a randomized block design with two dietary treatments (CM and CG with 400 g/kg DM each). Goats in CG increased sulfur, NDF and CP intake and decreased starch intake as compared with those in CM. Goats that received CG diet had decreased dissolved hydrogen (dH₂) (P = 0.01) and dissolved methane yield and emission (dCH₄) (P = 0.001), while increased dH₂S both in the rumen and hindgut segments than those fed CM. Goats fed CG had higher (p < 0.01) gene copies of microbiota and cellulolytic bacteria, whereas starch utilizing bacterial species were less in the rumen and hindgut than those fed CM. Higher (P < 0.05) methanogenic diversity and abundances of Methanimicrococcus and Methanomicrobium were observed in goats that consumed CG, whilst containing lower Methanobrevibacter populations than those receiving CM. The study suggested that goats fed corn gluten improved the gene copies of microbiota and fibrolytic bacterial species while reducing starch utilizing species in the rumen and hindgut segments as compared with that fed cornmeal. Goats consuming corn gluten had a more enriched methanogenic diversity and reduced Methanobrevibacter, a contributor to CH₄ emissions, as compared with goats fed CM. Corn gluten could be used as an alternative feed to decrease the enteric CH₄ emission in ruminant production. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=dissolved%20gasses" title="dissolved gasses">dissolved gasses</a>, <a href="https://publications.waset.org/abstracts/search?q=methanogenesis" title=" methanogenesis"> methanogenesis</a>, <a href="https://publications.waset.org/abstracts/search?q=microbial%20community" title=" microbial community"> microbial community</a>, <a href="https://publications.waset.org/abstracts/search?q=metagenomics" title=" metagenomics"> metagenomics</a> </p> <a href="https://publications.waset.org/abstracts/147510/sulfur-containing-diet-shift-hydrogen-metabolism-and-reduce-methane-emission-and-modulated-gut-microbiome-in-goats" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/147510.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">158</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4</span> Congenital Positional Anomaly of Descending Colon and Sigmoid Colon: Its Embryological Basis and Clinical Implications</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Dhivyalakshmi%20Gnanasekaran">Dhivyalakshmi Gnanasekaran</a>, <a href="https://publications.waset.org/abstracts/search?q=Sonali%20Adole%20Prasante"> Sonali Adole Prasante</a>, <a href="https://publications.waset.org/abstracts/search?q=Raveendranath%20Veeramamani"> Raveendranath Veeramamani</a>, <a href="https://publications.waset.org/abstracts/search?q=H.%20Y.%20Suma"> H. Y. Suma</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A rare case of intestinal malrotation with midline descending colon and right sided sigmoid colon was observed in an adult male cadaver aged around 55 years during routine dissection. The descending colon began from the splenic flexure and gradually descended downwards to occupy the midline position and turned to the right side to be continued as sigmoid colon at the level of the fifth lumbar vertebra. In the right iliac fossa some part of loop of sigmoid colon displaced into the right lumbar region before entering into the true pelvis to continue as rectum. This anomalous descending and sigmoid colon was supplied by varying branching pattern of inferior mesenteric artery. It is extremely important to consider this embryological anomaly before any interventional diagnostic procedures like colonoscopy and to enhance the safety of colonic surgery. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=sigmoid%20colon" title="sigmoid colon">sigmoid colon</a>, <a href="https://publications.waset.org/abstracts/search?q=descending%20colon" title=" descending colon"> descending colon</a>, <a href="https://publications.waset.org/abstracts/search?q=hindgut" title=" hindgut"> hindgut</a>, <a href="https://publications.waset.org/abstracts/search?q=malrotation" title=" malrotation"> malrotation</a> </p> <a href="https://publications.waset.org/abstracts/71406/congenital-positional-anomaly-of-descending-colon-and-sigmoid-colon-its-embryological-basis-and-clinical-implications" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/71406.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">251</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3</span> Sex-Dependent Fitness Improvement of Hercules Beetle Larvae by Amendment of Thermophile-Fermented Compost to Humus</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Futo%20Asano">Futo Asano</a>, <a href="https://publications.waset.org/abstracts/search?q=Yusuke%20Yatsushiro"> Yusuke Yatsushiro</a>, <a href="https://publications.waset.org/abstracts/search?q=Hirokuni%20Miyamoto"> Hirokuni Miyamoto</a>, <a href="https://publications.waset.org/abstracts/search?q=Hiroaki%20Kodama"> Hiroaki Kodama</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A thermophile-fermented compost is produced using small fishes, crabs, and shrimps under a high temperature (approximately 75℃) by fermentation-associated self-heating. This compost has been used as a feed additive for pigs and hens in Japan, and the fecundity of this livestock is enhanced. Firmicutes is a dominant phylum in the microbial composition of the compost. We first reported that improvement of female larval fitness of Hercules beetle can be achieved by amendment of this compost to the humus. When the 90-d-old larvae were reared for subsequent 72 days in the humus with this compost, the growth of female larvae was significantly enhanced when compared with the growth of female larvae in the humus without the compost. In contrast, the growth of male larvae in the compost-free humus was the same as the larvae grow in the compost-amended humus. The bacterial composition of the feces of larvae was determined at 0 days and 46 days after transfer to the humus with or without the compost. The most dominant bacterium in the feces was Xylanimonas. Interestingly, the growth improvement of female larvae was associated with an increased abundance of Mollicutes in the fecal samples. These results indicate that the compost act as a probiotic material for enhancing the female larvae growth by supporting Mollicutes. Here, we tried to isolate Mollicutes from the contents of the midgut and hindgut of the 3rd instar female larvae of the Hercules beetle. These gut contents were spread onto a selective agar medium for Mollicutes (PPLO agar broth, BD Difco, NJ, USA). Although we isolated none of the Mollicutes until now, several bacteria that are closely related to Xylanimonas and Luteimicrobium were isolated. These isolates have xylanase and glucanase (CMCase) activities. We show the gut bacterial profiles of larvae and discuss how the fitness of female larvae of the Hercules beetle is improved by the compost. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=compost" title="compost">compost</a>, <a href="https://publications.waset.org/abstracts/search?q=beetle" title=" beetle"> beetle</a>, <a href="https://publications.waset.org/abstracts/search?q=mollicutes" title=" mollicutes"> mollicutes</a>, <a href="https://publications.waset.org/abstracts/search?q=woody%20biomass" title=" woody biomass"> woody biomass</a> </p> <a href="https://publications.waset.org/abstracts/156759/sex-dependent-fitness-improvement-of-hercules-beetle-larvae-by-amendment-of-thermophile-fermented-compost-to-humus" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/156759.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">84</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2</span> Nitrogen Fixation in Hare Gastrointestinal Tract</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Tatiana%20A.%20Kuznetsova">Tatiana A. Kuznetsova</a>, <a href="https://publications.waset.org/abstracts/search?q=Maxim%20V.%20Vechersky"> Maxim V. Vechersky</a>, <a href="https://publications.waset.org/abstracts/search?q=Natalia%20V.%20Kostina"> Natalia V. Kostina</a>, <a href="https://publications.waset.org/abstracts/search?q=Marat%20M.%20Umarov"> Marat M. Umarov</a>, <a href="https://publications.waset.org/abstracts/search?q=Elena%20I.%20Naumova"> Elena I. Naumova</a> </p> <p class="card-text"><strong>Abstract:</strong></p> One of the main problems of nutrition of phytophagous animals is the insufficiency of protein in their forage. Usually, symbiotic microorganisms highly contribute both to carbohydrates and nitrogen compounds of the food. But it is not easy to utilize microbial biomass in the large intestine and caecum for the animals with hindgut fermentation. So that, some animals, as well hares, developed special mechanism of contribution of such biomass - obligate autocoprophagy, or reingestion. Hares have two types of feces - the hard and the soft. Hard feces are excreted at night, while hares are vigilance ("foraging period"), and the soft ones (caecotrophs) are produced and reingested in the day-time during hares "resting-period". We examine the role of microbial digestion in providing nitrogen nutrition of hare (Lepus europaeus). We determine the ability of nitrogen fixation in fornix and stomach body, small intestine, caecum and colon of hares' gastro-intestinal tract in two main period of hares activity - "resting-period" (day time) and "foraging period" (late-evening and very-early-morning). We use gas chromatography to measure levels of nitrogen fixing activity (acetylene reduction). Nitrogen fixing activity was detected in the contents of all analyzed parts of the gastrointestinal tract. Maximum values were recorded in the large intestine. Also daily dynamics of the process was detected. Thus, during hare “resting-period” (caecotrophs formation) N2-fixing activity was significantly higher than during “foraging period”, reaching 0,3 nmol C2H4/g*h. N2-fixing activity in the gastrointestinal tract can allocate to significant contribution of nitrogen fixers to microbial digestion in hare and confirms the importance of coprophagy as a nitrogen source in lagomorphs. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=coprophagy" title="coprophagy">coprophagy</a>, <a href="https://publications.waset.org/abstracts/search?q=gastrointestinal%20tract" title=" gastrointestinal tract"> gastrointestinal tract</a>, <a href="https://publications.waset.org/abstracts/search?q=lagomorphs" title=" lagomorphs"> lagomorphs</a>, <a href="https://publications.waset.org/abstracts/search?q=nitrogen%20fixation" title=" nitrogen fixation"> nitrogen fixation</a> </p> <a href="https://publications.waset.org/abstracts/29193/nitrogen-fixation-in-hare-gastrointestinal-tract" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/29193.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">364</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1</span> Changes of Mitochondrial Potential in the Midgut Epithelium of Lithobius forficatus (Myriapoda, Chilopoda) Exposed to Cadmium Concentrated in Soil</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Magdalena%20Rost-Roszkowska">Magdalena Rost-Roszkowska</a>, <a href="https://publications.waset.org/abstracts/search?q=Izabela%20Poprawa"> Izabela Poprawa</a>, <a href="https://publications.waset.org/abstracts/search?q=Alina%20Chachulska-Zymelka"> Alina Chachulska-Zymelka</a>, <a href="https://publications.waset.org/abstracts/search?q=Lukasz%20Chajec"> Lukasz Chajec</a>, <a href="https://publications.waset.org/abstracts/search?q=Grazyna%20Wilczek"> Grazyna Wilczek</a>, <a href="https://publications.waset.org/abstracts/search?q=Piotr%20Wilczek"> Piotr Wilczek</a>, <a href="https://publications.waset.org/abstracts/search?q=Malgorzata%20Lesniewska"> Malgorzata Lesniewska</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Lithobius forficatus, commonly known as the brown centipede, is a widespread European species, which lives in the upper layers of soil, under stones, litter, rocks, and leaves. As the soil organism, it is exposed to numerous stressors such as xenobiotics, including heavy metals, temperature, starvation, pathogens, etc. Heavy metals are treated as the environmental pollutants of the soil because of their toxic effects on plants, animals and human being. One of the heavy metals which is xenobiotic and can be taken up by plants or animals from the soil is cadmium. The digestive system of centipedes is composed of three distinct regions: fore-, mid- and hindgut. The salivary glands of centipedes are the organs which belong to the anterior region of the digestive system and take part in the synthesis, accumulation, and secretion of many substances. The middle region having contact with the food masses is treated as one of the barriers which protect the organism against any stressors which originate from the external environment, e.g., toxic metals. As the material for our studies, we chose two organs of the digestive system in brown centipede, the organs which take part in homeostasis maintenance: the salivary glands and the midgut. The main purpose of the project was to investigate the relationship between the percentage of depolarized mitochondria, mitophagy and ATP level in cells of mentioned above organs. The animals were divided into experimental groups: K – the control group, the animals cultured in a laboratory conditions in a horticultural soil and fed with Acheta domesticus larvae; Cd1 – the animals cultured in a horticultural soil supplemented with 80 mg/kg (dry weight) of CdCl2, fed with A. domesticus larvae maintained in tap water, 12 days – short-term exposure; Cd2 – the animals cultured in a horticultural soil supplemented with 80 mg/kg (dry weight) of CdCl2, fed with A. domesticus larvae maintained in tap water, 45 days – long-term exposure. The studies were conducted using transmission electron microscopy (TEM), flow cytometry and confocal microscopy. Quantitative analysis revealed that regardless of the organ, a progressive increase in the percentage of cells with depolarized mitochondria was registered, but only in the salivary glands. These were statistically significant changes from the control. In both organs, there were no differences in the level of the analyzed parameter depending on the duration of exposure of individuals to cadmium. Changes in the ultrastructure of mitochondria have been observed. With the extension of the body's exposure time to metal, an increase in the ADP/ATP index was recorded. However, changes statistically significant to the control were demonstrated in the intestine and salivary glands. The size of this intestinal index and salivary glands in the Cd2 group was about thirty and twenty times higher, respectively than in control. Acknowledgment: The study has been financed by the National Science Centre, Poland, grant no 2017/25/B/NZ4/00420. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cadmium" title="cadmium">cadmium</a>, <a href="https://publications.waset.org/abstracts/search?q=digestive%20system" title=" digestive system"> digestive system</a>, <a href="https://publications.waset.org/abstracts/search?q=ultrastructure" title=" ultrastructure"> ultrastructure</a>, <a href="https://publications.waset.org/abstracts/search?q=centipede" title=" centipede"> centipede</a> </p> <a href="https://publications.waset.org/abstracts/107844/changes-of-mitochondrial-potential-in-the-midgut-epithelium-of-lithobius-forficatus-myriapoda-chilopoda-exposed-to-cadmium-concentrated-in-soil" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/107844.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">136</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates its 16th foundational anniversary</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Account <li><a href="https://waset.org/profile">My Account</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Explore <li><a href="https://waset.org/disciplines">Disciplines</a></li> <li><a href="https://waset.org/conferences">Conferences</a></li> <li><a href="https://waset.org/conference-programs">Conference Program</a></li> <li><a href="https://waset.org/committees">Committees</a></li> <li><a href="https://publications.waset.org">Publications</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Research <li><a href="https://publications.waset.org/abstracts">Abstracts</a></li> <li><a href="https://publications.waset.org">Periodicals</a></li> <li><a href="https://publications.waset.org/archive">Archive</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Open Science <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Science-Philosophy.pdf">Open Science Philosophy</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Science-Award.pdf">Open Science Award</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Society-Open-Science-and-Open-Innovation.pdf">Open Innovation</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Postdoctoral-Fellowship-Award.pdf">Postdoctoral Fellowship Award</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Scholarly-Research-Review.pdf">Scholarly Research Review</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Support <li><a href="https://waset.org/page/support">Support</a></li> <li><a href="https://waset.org/profile/messages/create">Contact Us</a></li> <li><a href="https://waset.org/profile/messages/create">Report Abuse</a></li> </ul> </div> </div> </div> </div> </div> <div class="container text-center"> <hr style="margin-top:0;margin-bottom:.3rem;"> <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank" class="text-muted small">Creative Commons Attribution 4.0 International License</a> <div id="copy" class="mt-2">&copy; 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