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border-color; } /* Style new checked item */ ul.inputs-list.multi-container li.hs-form-checkbox label span::after, .hs-form-booleancheckbox-display span::after { content: ""; border: 2px solid #d9d9d9; border-radius: 3px; height: 20px; left: 0px; position: absolute; top: 0px; width: 20px; background: #fff; z-index: 0; display: block; transition-property: background-color, border-color, -webkit-box-shadow; -o-transition-property: background-color, border-color, box-shadow; transition-property: background-color, border-color, box-shadow; transition-property: background-color, border-color, box-shadow, -webkit-box-shadow; } /* Show when checked */ ul.inputs-list.multi-container li.hs-form-checkbox label input:checked + span::after, .hs-form-booleancheckbox-display input:checked + span::after, ul.inputs-list.multi-container li.hs-form-checkbox label input:checked + span::before, .hs-form-booleancheckbox-display input:checked + span::before{ opacity: 1; } .hs-form-booleancheckbox-display input:checked+label:after, .hs-form-booleancheckbox-display input:checked+label:after { background: #fff; border-color: #157479; } /* Style when focused */ ul.inputs-list.multi-container li.hs-form-checkbox label input:focus + span::after, .hs-form-booleancheckbox-display input:focus + span::after { outline: 3px solid transparent; /* For Windows high contrast mode. */ box-shadow: 0 0 .3125rem rgba(0, 0, 0, .3); border-color: #157479; } .hs_contact_country{ clear: both; } .hs-button { border-radius: 65px !important; font-size: 14px; line-height: 1.3; letter-spacing: 1.12px; font-family: "Roboto Slab", helvetica, arial, sans-serif; font-weight: 700 !important; padding: 19px 23px !important; border: 2px solid; display: inline-block; text-decoration: none; text-transform: uppercase; -webkit-appearance: none !important; -moz-appearance: none !important; appearance: none !important; } .hbspt-form textarea{max-width: 100% !important; height: 186px !important; resize: none;} .hbspt-form textarea:focus{border: 1px solid #74cbc8 !important;} .hs-fieldtype-textarea .invalid.error{border: 2px solid #E74949 !important;} .hbspt-form fieldset{max-width: 100% !important; margin-bottom: 60px;} .hbspt-form .field {margin-bottom: 27px} .hs_recaptcha{ margin-top: 23px !important; margin-bottom: 27px !important; } .hs-button, .hs-form-field input[type=text], .hs-form-field input[type=email], .hs-form-field input[type=phone], .hs-form-field input[type=number], .hs-form-field input[type=tel], .hs-form-field input[type=date], .hs-form-field textarea { appearance: none; -webkit-appearance: none; -moz-appearance: none; } .hs-default-font-element, .hs-main-font-element { font-family: "Roboto Slab", helvetica, arial, sans-serif; } .hbspt-form .hs-button { white-space: pre-wrap } .hbspt-form .hs-richtext { word-break: break-word } .hbspt-form.submitted-message { overflow: auto } .hbspt-form fieldset.form-columns-1 .hs-input { width: 95% } .hbspt-form fieldset.form-columns-1 .input { margin-right: 0px !important } .hbspt-form fieldset.form-columns-1 input[type=checkbox], .hbspt-form fieldset.form-columns-1 input[type=radio] { width: auto } .hbspt-form fieldset.form-columns-2 .hs-form-field { width: 50%; float: left } .hbspt-form fieldset.form-columns-2 .input { margin-right: 0px !important; } .hbspt-form fieldset.form-columns-3 .hs-form-field { width: 32.7%; float: left } .hbspt-form fieldset.form-columns-3 .input { margin-right: 8px } .hbspt-form label.hs-hidden { visibility: hidden } .hbspt-form .hs-field-desc { width: 100% } .hs-custom-style .hs-input, .hs-custom-style fieldset { max-width: 100% } .hs-custom-style>div.form-columns-3 .hs-form-field, .hs-custom-style fieldset.form-columns-3 .hs-form-field { width: 33.3% } .hs-custom-style>div>div:last-of-type .hs-input:not([type=checkbox]):not([type=radio]), .hs-custom-style fieldset>div:last-of-type .hs-input:not([type=checkbox]):not([type=radio]) { width: 100%; max-width: 100% } .hs-custom-style>div input:not([type=image]):not([type=submit]):not([type=button]):not([type=radio]):not([type=checkbox]):not([type=file]), .hs-custom-style fieldset input:not([type=image]):not([type=submit]):not([type=button]):not([type=radio]):not([type=checkbox]):not([type=file]) { box-sizing: border-box; padding: 0 15px; min-height: 27px } .hs-custom-style>div textarea, .hs-custom-style fieldset textarea { padding: 10px 15px } .hs-custom-style .hs-dependent-field>div .hs-input:not([type=checkbox]):not([type=radio]) { width: 100% } .legal-consent-container .field.hs-form-field { margin-bottom: 8px } .legal-consent-container .hs-field-desc.checkbox-desc { margin: -12px 0 0 21px } .legal-consent-container .hs-form-booleancheckbox-display input { float: left } .legal-consent-container .hs-form-booleancheckbox-display>span { display: block !important; margin-left: 30px !important; } .legal-consent-container .hs-form-booleancheckbox-display p { margin: 0; display: inline } .legal-consent-container .hs-error-msgs label { color: #f2545b } .legal-consent-container~.hs_recaptcha { margin-top: 18px } .cookie-reset-container { font-size: 14px; margin-bottom: 10px; text-align: right } #hs-outer-captcha-target, #hs-outer-captcha-target * { display: none; height: 0; width: 0 } .hubspot-link__container { font-size: 14px; padding-bottom: 40px; position: relative; color: #9fa0a2; font-family: "Roboto Slab", helvetica, arial, sans-serif; } .hubspot-link-text { color: #00a4bd; font-weight: 400 } .hubspot-link__container.sproket { color: #9fa0a2 } .hubspot-link { color: #9fa0a2 } .hubspot-link, .hubspot-link:hover { text-decoration: none } .hubspot-link:hover .hubspot-link-text { text-decoration: underline } .hubspot-link__icon { margin-bottom: -1px; margin-right: 5px } .hubspot-link__container.sproket .hubspot-link__icon { width: 30px; margin-right: 0; float: left; margin-top: -9px; margin-left: -5px } .hubspot_signup_form .hs_email{margin-bottom: 0px !important; width: 80% !important; float: left !important;} .hubspot_signup_form .hs_submit{width: 20% !important; float: left !important; } .hubspot_signup_form .hs-button{padding: 12.5px 15px !important;} .hubspot_signup_form .hs_email label{display: none !important;} .hubspot_signup_form ul.no-list{position: absolute !important; top: 48px !important;} .hubspot_signup_form {position: relative !important;} .hubspot_signup_form .submitted-message{color: #ab8940 !important; font-size: 22px !important; } .hubspot_white .hbspt-form label, .hubspot_white .hs-form-required { color: #fff; } .hubspot_white .hs-input, .hubspot_white ul.inputs-list.multi-container li.hs-form-checkbox label span::before, .hubspot_white .hs-form-booleancheckbox-display span::before{ background-color: #fff !important; } .hubspot_white .hs_submit { text-align: center; } .hubspot_white .hs-form select.is-placeholder { opacity: 1; } .hubspot_white ul.inputs-list.multi-container li.hs-form-checkbox label span::after, .hubspot_white .hs-form-booleancheckbox-display span::after { border-color: #3f4852; } input[type=submit].hs-button.primary.large{ color: #fff; background: #00181e; border-color: #00181e !important; } input[type=submit].hs-button.primary.large:hover { background: #74cbc8 !important; border-color: #74cbc8 !important; } /***/ .hbspt-form .hs-input[type=checkbox]{ cursor: pointer; } .hs-form-booleancheckbox-display input:checked + span::after{ background: #fff; border-color: #157479; } .hbspt-form .form-columns-2 { display: flex; flex-flow: row wrap; -webkit-box-direction: normal; } .hbspt-form .hs_firstname { flex: 1; -webkit-box-flex: 1; } .hbspt-form .hs_submit{ margin-top: 60px; } .newsletter-form .hs_submit{margin-top: 45px;} .newsletter-form form .gfield_consent_description { border: none !important; } /*New Landing page*/ .landing-middle-section{ padding-right: 0px !important; } .left-col{max-width:93%} .left-col-para{padding-right:30px;} .left-col video{border-radius:4px;} /* Kit asssesment */ .kits_form_col .hbspt-form .hs-input[type=checkbox] { height: 20px !important; width: 20px !important; } .kits_form_col {padding-bottom: 45px;} .hs-richtext h4 {margin-bottom: 15px !important;} .kits_form_col label.hs-form-checkbox-display {margin-bottom: 15px;} .kits_form_col .hbspt-form li.hs-form-checkbox label span{ margin-left: 0px !important; padding-left: 31px; } .hbspt-form label.hs-form-radio-display { position: relative; padding: 4px 31px; } .hbspt-form .hs-form-radio-display span, .hbspt-form .hs-form-checkbox-display span { position: relative; cursor: pointer; font-size: 15px; font-weight: 300; } .hbspt-form .hs-input[type=radio] { position: absolute; left: 0; z-index: 2; width: 20px !important; height: 20px !important; outline: none; cursor: pointer; background-color: transparent !important; border-radius: 50%; } .hbspt-form .hs_protocol_synopsis_upload .input{position: relative;} .hbspt-form input[type="file"]::-webkit-file-upload-button { cursor: pointer; background-color: #157479; padding: 10px 20px !important; border: 1px solid #157479; border-top-left-radius: 0px; border-bottom-left-radius: 0px; border-top-right-radius: 6px; border-bottom-right-radius: 6px; color: #fff; position: absolute; top: 0; bottom: 0; right: 0; } .hbspt-form input[type="file"] { cursor: pointer; padding: 10px 20px !important; position: relative; top: 0; bottom: 0; right: 0; } /*Form Responsive CSS*/ @media only screen and (min-width: 1024px) and (max-width: 1366px){ .hs_email, .hs_contact_country { clear: both; } } @media only screen and (min-width: 992px) and (max-width: 1024px){ .hs_email, .hs_contact_country { clear: both; } } @media (min-width: 640px) and (max-width: 1120px) { .hbspt-form h1{font-size: 36px !important;} } @media screen and (min-width: 1120px) { .hbspt-form h1{font-size: 45px !important;margin-bottom: 17px !important;} .hbspt-form .gform_heading p{font-size: 17px !important;} } @media only screen and (min-width: 768px) and (max-width: 992px){ .hs_email, .hs_contact_country{ clear: both; } } @media (min-width: 767px) and (max-width: 1024px) { body .hubspot_signup_form .hs_email{width: 66% !important; float: left !important;} } @media screen and (max-width: 992px){ .assay-kit-hbspt-form .fl-col { width: 100%; } } @media screen and (max-width:812px){ .assay-kit-hbspt-form .fl-col { max-width: 100%; } .hbspt-form .hs-fieldtype-select .input:before{ right: 20px !important; } } @media screen and (max-width: 767px){ .hs_submit .actions{padding-top: 0px !important; margin-top: 0px !important;} body .hbspt-form fieldset.form-columns-2 .input {margin-right: 0px !important;} body fieldset.form-columns-3 .input{margin-right: 0px !important;} .hs-form-field .hs-input { width: 100% !important; } .hs-fieldtype-textarea .input{margin-right: 0px !important;} .hs_firstname, .hs_phone, .hs_development_area{ width: 100% !important; } } @media screen and (min-width: 640px){ .hbspt-form .hs_firstname, .kits_form_col .hs_email{ 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href="https://www.bioagilytix.com/speak-to-scientist/">Speak to a Scientist</a></div></div></div><div class="breadcrumbs"><span><span><a href="https://www.bioagilytix.com/"><i class="home-icon"></i><span class="screen-reader-text">Home</span></a></span> <span class="breadcrumb-separator"></span> <span><a href="https://www.bioagilytix.com/techniques/">Techniques & Platforms</a></span> <span class="breadcrumb-separator"></span> <span><a href="https://www.bioagilytix.com/techniques/assay-services/">Techniques</a></span> <span class="breadcrumb-separator"></span> <span class="breadcrumb_last" aria-current="page">Molecular Assays</span></span></div></div></section><div class="wp-block-group alignwide is-layout-constrained wp-container-core-group-is-layout-1 wp-block-group-is-layout-constrained"><p class="has-text-align-center has-large-font-size">Molecular assays are commonly used in support of drug development, particularly with newer drug modalities such as cell and gene therapies. BioAgilytix leverages real-time polymerase chain reaction, quantitative PCR or qPCR, and Droplet Digital™ PCR (ddPCR™) as part of our extensive molecular technology suite to deliver bioanalytical solutions to support discovery, preclinical, and clinical programs.</p></div><div id="block_91f6e593d0029b382cb1626fd5ae7ae8" class="wp-block em-media-content em-mt--lg alignfull"><div class="em-media-content__inner em-media-content__inner--media-right has-gray-xxl-background-color em-media-content__inner--content-has-padding"><div class="em-media-content__media em-media-content__media--right"> <img fetchpriority="high" decoding="async" src="https://www.bioagilytix.com/wp-content/uploads/2023/09/iStock-177381768-1-1380x776.jpg" alt="gloved hand prepping lab equipment" class="object-fit--cover" srcset="https://www.bioagilytix.com/wp-content/uploads/2023/09/iStock-177381768-1-1380x776.jpg 1380w, https://www.bioagilytix.com/wp-content/uploads/2023/09/iStock-177381768-1-2000x1125.jpg 2000w, https://www.bioagilytix.com/wp-content/uploads/2023/09/iStock-177381768-1-1120x630.jpg 1120w, https://www.bioagilytix.com/wp-content/uploads/2023/09/iStock-177381768-1-850x478.jpg 850w, https://www.bioagilytix.com/wp-content/uploads/2023/09/iStock-177381768-1-640x360.jpg 640w" width="1380" height="776"></div><div class="em-media-content__content em-media-content__content--left em-media-content__content--add-padding"><h2 class="h2">BioAgilityx’s PCR expertise</h2><p class="">Our scientific team offers a breadth and depth of expertise in the development, optimization, and validation of complex molecular assays using qPCR and ddPCR, collaborating with your team to ensure your project is a success. Our laboratories are equipped with the latest generation of PCR equipment, including Applied Biosystems QuantStudio 7 Flex or Pro with both 96- and 384-well fast-cycle options, as well as TaqMan Array Cards and BioRad’s droplet digital PCR systems, QX200 and QXOne. </p></div></div></div><div id="block_651a3da5814ae84ad15db57c9f426612" class="wp-block em-media-content em-mt--lg em-mb--xl alignwide"><div class="em-media-content__inner em-media-content__inner--media-left has-none-background-color em-media-content__inner--content-has-padding"><div class="em-media-content__media em-media-content__media--left"> <noscript><img decoding="async" src="https://www.bioagilytix.com/wp-content/uploads/2023/09/iStock-665525354.jpg" alt="Multichannel pipette tips filled in with reaction mixture" class="object-fit--cover" srcset="https://www.bioagilytix.com/wp-content/uploads/2023/09/iStock-665525354.jpg 1255w, https://www.bioagilytix.com/wp-content/uploads/2023/09/iStock-665525354-300x200.jpg 300w, https://www.bioagilytix.com/wp-content/uploads/2023/09/iStock-665525354-1024x681.jpg 1024w, https://www.bioagilytix.com/wp-content/uploads/2023/09/iStock-665525354-768x511.jpg 768w, https://www.bioagilytix.com/wp-content/uploads/2023/09/iStock-665525354-100x67.jpg 100w" width="1255" height="835"></noscript><img decoding="async" src='data:image/svg+xml,%3Csvg%20xmlns=%22http://www.w3.org/2000/svg%22%20viewBox=%220%200%201255%20835%22%3E%3C/svg%3E' data-src="https://www.bioagilytix.com/wp-content/uploads/2023/09/iStock-665525354.jpg" alt="Multichannel pipette tips filled in with reaction mixture" class="lazyload object-fit--cover" data-srcset="https://www.bioagilytix.com/wp-content/uploads/2023/09/iStock-665525354.jpg 1255w, https://www.bioagilytix.com/wp-content/uploads/2023/09/iStock-665525354-300x200.jpg 300w, https://www.bioagilytix.com/wp-content/uploads/2023/09/iStock-665525354-1024x681.jpg 1024w, https://www.bioagilytix.com/wp-content/uploads/2023/09/iStock-665525354-768x511.jpg 768w, https://www.bioagilytix.com/wp-content/uploads/2023/09/iStock-665525354-100x67.jpg 100w" width="1255" height="835"></div><div class="em-media-content__content em-media-content__content--right em-media-content__content--add-padding"><h2 class="h2"><strong><strong>PCR for molecular assay support at any phase of drug development</strong></strong></h2><p class="">Common PCR-based methods supported by BioAgilytix include:</p><ul class="wp-block-list"><li class="">Custom, real-time <a href="https://www.bioagilytix.com/techniques/instrumentation/qpcr-ddpcr-assay/">qPCR</a></li><li class="">Gene expression analysis using TaqMan assays</li><li class="">SNP genotyping analysis and copy number variation</li><li class="">Mutation detection </li><li class=""><a href="https://go.bioagilytix.com/rna-therapy-bioanalysis">PK of RNA-based therapeutics</a></li><li class="">Gene and cell therapy support (biodistribution/persistence, viral shedding)</li></ul></div></div></div><div class="wp-block-group alignwide em-mt--lg em-mb--md is-content-justification-left is-layout-constrained wp-container-core-group-is-layout-2 wp-block-group-is-layout-constrained"><h2 class="h2"><strong>Selecting qPCR or ddPCR for high-quality molecular results</strong></h2><p class="">Molecular assays in support of bioanalytical testing can be performed on either qPCR or ddPCR. Real-time PCR (RT-qPCR or qPCR) is often considered the gold standard in molecular analysis for detecting nucleic acids. However, ddPCR is a newer technology that can have distinct advantages over traditional PCR methods in specific situations.  </p></div><div class="wp-block-emagine-grid alignwide wp-block-emagine-grid--halves"><div class="wp-block-emagine-grid__inner em-grid-2_sm-1-left-middle"><div class="wp-block-emagine-column em-col-middle"><div class="wp-block-image size-large"><div><figure class=""><noscript><img decoding="async" width="1024" height="945" src="https://www.bioagilytix.com/wp-content/uploads/2023/09/MG_Right.jpg-650-transformed-1024x945.jpg" alt="" class="wp-image-5802" srcset="https://www.bioagilytix.com/wp-content/uploads/2023/09/MG_Right.jpg-650-transformed-1024x945.jpg 1024w, https://www.bioagilytix.com/wp-content/uploads/2023/09/MG_Right.jpg-650-transformed-300x277.jpg 300w, https://www.bioagilytix.com/wp-content/uploads/2023/09/MG_Right.jpg-650-transformed-768x709.jpg 768w, https://www.bioagilytix.com/wp-content/uploads/2023/09/MG_Right.jpg-650-transformed-100x92.jpg 100w, https://www.bioagilytix.com/wp-content/uploads/2023/09/MG_Right.jpg-650-transformed.jpg 1300w" sizes="(max-width: 1024px) 100vw, 1024px" /></noscript><img decoding="async" width="1024" height="945" src='data:image/svg+xml,%3Csvg%20xmlns=%22http://www.w3.org/2000/svg%22%20viewBox=%220%200%201024%20945%22%3E%3C/svg%3E' data-src="https://www.bioagilytix.com/wp-content/uploads/2023/09/MG_Right.jpg-650-transformed-1024x945.jpg" alt="" class="lazyload wp-image-5802" data-srcset="https://www.bioagilytix.com/wp-content/uploads/2023/09/MG_Right.jpg-650-transformed-1024x945.jpg 1024w, https://www.bioagilytix.com/wp-content/uploads/2023/09/MG_Right.jpg-650-transformed-300x277.jpg 300w, https://www.bioagilytix.com/wp-content/uploads/2023/09/MG_Right.jpg-650-transformed-768x709.jpg 768w, https://www.bioagilytix.com/wp-content/uploads/2023/09/MG_Right.jpg-650-transformed-100x92.jpg 100w, https://www.bioagilytix.com/wp-content/uploads/2023/09/MG_Right.jpg-650-transformed.jpg 1300w" data-sizes="(max-width: 1024px) 100vw, 1024px" /></figure></div></div></div><div class="wp-block-emagine-column em-col-middle"><h3 class="h3">Applications for qPCR</h3><p class="">qPCR monitors DNA amplification in real-time by continually measuring the signal of fluorescent molecules (chromophores) activated during the PCR process. Modern qPCR instrumentation allows for the simultaneous measurement of multiple, unique DNA amplifications in a single reaction vessel, making it possible to quantitate multiple targets per sample. This translates into lower sample volumes and reduced reagent usage, creating substantial overall time and cost savings. BioAgilytix uses the latest Quant Studio platforms that are scalable with both 96- or 384-well blocks. The qPCR platform can obtain 10 logs of linear range and is useful with assays that require wide dynamic ranges.</p></div></div></div><div id="block_77616b86f1f6635c584af98b9f0307e4" class="wp-block em-media-content em-mt--none alignwide"><div class="em-media-content__inner em-media-content__inner--media-right has-none-background-color em-media-content__inner--content-has-padding"><div class="em-media-content__media em-media-content__media--right"> <noscript><img decoding="async" src="https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1907619679.jpg" alt="DNA rendering" class="object-fit--cover" srcset="https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1907619679.jpg 1000w, https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1907619679-300x169.jpg 300w, https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1907619679-768x432.jpg 768w, https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1907619679-850x478.jpg 850w, https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1907619679-640x360.jpg 640w, https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1907619679-100x56.jpg 100w" width="1000" height="563"></noscript><img decoding="async" src='data:image/svg+xml,%3Csvg%20xmlns=%22http://www.w3.org/2000/svg%22%20viewBox=%220%200%201000%20563%22%3E%3C/svg%3E' data-src="https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1907619679.jpg" alt="DNA rendering" class="lazyload object-fit--cover" data-srcset="https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1907619679.jpg 1000w, https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1907619679-300x169.jpg 300w, https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1907619679-768x432.jpg 768w, https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1907619679-850x478.jpg 850w, https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1907619679-640x360.jpg 640w, https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1907619679-100x56.jpg 100w" width="1000" height="563"></div><div class="em-media-content__content em-media-content__content--left em-media-content__content--add-padding"><h3 class="h3">Applications for ddPCR</h3><p class="">ddPCR is inherently more precise than traditional PCR as ddPCR amplifies individual DNA fragments rather than from bulk sampled DNA. In ddPCR, samples are fractionated into thousands of individual droplets. Individual DNA fragments in the sample are thereby partitioned or distributed within the droplets which allows direct counting of the number of DNA molecules within a sample (i.e. the number of droplets in the sample that contain DNA).  This method provides high precision at low copy numbers. BioAgilytix uses ddPCR QX200 and QX One for absolute quantification of target DNA or RNA (cDNA) molecules without the need for a standard curve. QX ONE ddPCR System is used for its multiplexing capabilities to simultaneously detect multiple targets. This system seamlessly integrates a standard ddPCR workflow of droplet generation, thermal cycling, droplet reading, and analysis into a robust platform. The walk-away automation of the QX ONE allows our scientists to provide robust results. </p></div></div></div></article></main><footer class="site-footer site-footer--has-logo site-footer--align-brand-left"><div class="deep-footer clearfix"><div class="alignwide"><div class="em-grid"><div class="em-col-6_md-12"><div class="deep-footer__brand"><div class="deep-footer__logo" style="max-width: 300px"> <a href="https://www.bioagilytix.com"><div class="em-image"><div class="em-image__ratio" style="padding-top:21.420572708367%;"> <svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 187.53 40.17" width="187.53px" height="40.17px" class=""><g fill="none"><g fill="#fff"><path d="M12.04 18.13v-.14c2.53-1.16 3.8-2.91 3.8-5.23 0-.87-.16-1.61-.49-2.24s-.78-1.11-1.36-1.45c-.64-.38-1.4-.65-2.28-.8-.66-.11-1.51-.17-2.56-.17H0v1.02c.35.1.63.22.83.35s.35.29.45.5.16.47.18.8.04.73.04 1.22v13.3c0 .5-.01.9-.04 1.23-.03.32-.09.59-.19.8s-.25.38-.45.5-.47.23-.82.33v1.02h8.62c1.33 0 2.43-.11 3.29-.34s1.61-.58 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