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data-elementor-type="wp-page" data-elementor-id="92" class="elementor elementor-92"> <div class="elementor-element elementor-element-5d54b52 e-flex e-con-boxed e-con e-parent" data-id="5d54b52" data-element_type="container" data-settings="{"background_background":"classic"}"> <div class="e-con-inner"> <div class="elementor-element elementor-element-45abeee elementor-widget elementor-widget-heading" data-id="45abeee" data-element_type="widget" data-widget_type="heading.default"> <div class="elementor-widget-container"> <h1 class="elementor-heading-title elementor-size-default">Custom Real-Time PCR (qPCR)</h1> </div> </div> <div class="elementor-element elementor-element-617d3af elementor-widget elementor-widget-heading" data-id="617d3af" data-element_type="widget" data-widget_type="heading.default"> <div class="elementor-widget-container"> <h2 class="elementor-heading-title elementor-size-default">Our Real-Time PCR (qPCR) service encompasses the complete workflow, from primer design to PCR data analysis. <br>We work closely with you to tailor the process to your specific needs and make any adjustments needed to achieve the best possible results.</br></h2> </div> </div> </div> </div> <div class="elementor-element elementor-element-d7c30d8 e-flex e-con-boxed e-con e-parent" data-id="d7c30d8" data-element_type="container" data-settings="{"background_background":"classic"}"> <div class="e-con-inner"> <div class="elementor-element elementor-element-8550022 e-flex e-con-boxed e-con e-child" data-id="8550022" data-element_type="container"> <div class="e-con-inner"> <div class="elementor-element elementor-element-ee9d457 elementor-widget elementor-widget-heading" data-id="ee9d457" data-element_type="widget" data-widget_type="heading.default"> <div class="elementor-widget-container"> <h3 class="elementor-heading-title elementor-size-default">Quantitative PCR (qPCR), also known as real-time PCR, is a powerful and widely-used molecular biology technique that enables the precise detection and quantification of nucleic acids. It has revolutionized research and diagnostic workflows across fields such as gene expression analysis, pathogen detection, and genetic variation studies. qPCR combines the specificity and amplification efficiency of traditional PCR with real-time detection, providing a reliable and highly sensitive approach to monitor DNA or RNA in diverse applications. This is made possible by state-of-the-art </h3> </div> </div> </div> </div> <div class="elementor-element elementor-element-f852632 e-con-full e-flex e-con e-child" data-id="f852632" data-element_type="container"> <div class="elementor-element elementor-element-7af452d elementor-widget elementor-widget-heading" data-id="7af452d" data-element_type="widget" data-widget_type="heading.default"> <div class="elementor-widget-container"> <h3 class="elementor-heading-title elementor-size-default">instruments housed in our labs, including the CFX 96, QuantStudio 5-DX™, CFS Opus 96 - Dx, and Fluidigm Biomark™ HD. <br>qPCR offers exceptional sensitivity, specificity, and reproducibility, making it ideal for detecting low-abundance targets, quantifying gene expression, and monitoring genetic variations.<br>Whether you are engaged in genomics research, oncology studies, or infectious disease diagnostics, we deliver comprehensive real-time PCR (qPCR) services that provide the reliable data you need to advance your scientific endeavors.</h3> </div> </div> </div> </div> </div> <div class="elementor-element elementor-element-ddf33a7 e-flex e-con-boxed e-con e-parent" data-id="ddf33a7" data-element_type="container"> <div class="e-con-inner"> <div class="elementor-element elementor-element-8620701 elementor-widget elementor-widget-heading" data-id="8620701" data-element_type="widget" data-widget_type="heading.default"> <div class="elementor-widget-container"> <h2 class="elementor-heading-title elementor-size-default">Our product portfolio</h2> </div> </div> <div class="elementor-element elementor-element-2832ac3 elementor-widget-divider--view-line elementor-widget elementor-widget-divider" data-id="2832ac3" data-element_type="widget" data-widget_type="divider.default"> <div class="elementor-widget-container"> <div class="elementor-divider"> <span class="elementor-divider-separator"> </span> </div> </div> </div> <div class="elementor-element elementor-element-73101ac elementor-widget elementor-widget-text-editor" data-id="73101ac" data-element_type="widget" data-widget_type="text-editor.default"> <div class="elementor-widget-container"> <p>Our experts leverage cutting-edge technologies in our molecular biology labs to perform real-time PCR (qPCR) experiments with high sensitivity, specificity, and reproducibility. </p> </div> </div> </div> </div> <div class="elementor-element elementor-element-254e1ef e-flex e-con-boxed e-con e-parent" data-id="254e1ef" data-element_type="container"> <div class="e-con-inner"> <div class="elementor-element elementor-element-a9aef10 e-con-full e-flex e-con e-child" data-id="a9aef10" data-element_type="container"> </div> <div class="elementor-element elementor-element-e3901ed e-con-full e-flex e-con e-child" data-id="e3901ed" data-element_type="container"> <div class="elementor-element elementor-element-4817871 elementor-widget elementor-widget-image" data-id="4817871" data-element_type="widget" data-widget_type="image.default"> <div class="elementor-widget-container"> <img decoding="async" width="150" height="150" src="https://i0.wp.com/firalismolecularprecision.com/wp-content/uploads/2023/04/CFX.png?resize=150%2C150&ssl=1" class="attachment-thumbnail size-thumbnail wp-image-2183" alt="" data-attachment-id="2183" data-permalink="https://firalismolecularprecision.com/cro-cdmo-services/genomic-services/custom-rt-qpcr-services/cfx/" data-orig-file="https://i0.wp.com/firalismolecularprecision.com/wp-content/uploads/2023/04/CFX.png?fit=439%2C421&ssl=1" data-orig-size="439,421" data-comments-opened="1" data-image-meta="{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}" data-image-title="CFX" data-image-description="" data-image-caption="" data-medium-file="https://i0.wp.com/firalismolecularprecision.com/wp-content/uploads/2023/04/CFX.png?fit=300%2C288&ssl=1" data-large-file="https://i0.wp.com/firalismolecularprecision.com/wp-content/uploads/2023/04/CFX.png?fit=439%2C421&ssl=1" /> </div> </div> <div class="elementor-element elementor-element-9b6cec5 elementor-widget elementor-widget-heading" data-id="9b6cec5" data-element_type="widget" data-widget_type="heading.default"> <div class="elementor-widget-container"> <h2 class="elementor-heading-title elementor-size-default">CFX 96</h2> </div> </div> </div> <div class="elementor-element elementor-element-8b83ed8 e-con-full e-flex e-con e-child" data-id="8b83ed8" data-element_type="container"> <div class="elementor-element elementor-element-8b4f2d3 elementor-widget elementor-widget-image" data-id="8b4f2d3" data-element_type="widget" data-widget_type="image.default"> <div class="elementor-widget-container"> <img fetchpriority="high" decoding="async" width="640" height="497" src="https://i0.wp.com/firalismolecularprecision.com/wp-content/uploads/2023/04/QuantStudio-5-e1681219172931.png?fit=640%2C497&ssl=1" class="attachment-large size-large wp-image-2186" alt="" data-attachment-id="2186" data-permalink="https://firalismolecularprecision.com/cro-cdmo-services/genomic-services/custom-rt-qpcr-services/quantstudio-5/" data-orig-file="https://i0.wp.com/firalismolecularprecision.com/wp-content/uploads/2023/04/QuantStudio-5-e1681219172931.png?fit=650%2C505&ssl=1" data-orig-size="650,505" data-comments-opened="1" data-image-meta="{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}" data-image-title="QuantStudio-5" data-image-description="" data-image-caption="" data-medium-file="https://i0.wp.com/firalismolecularprecision.com/wp-content/uploads/2023/04/QuantStudio-5-e1681219172931.png?fit=300%2C233&ssl=1" data-large-file="https://i0.wp.com/firalismolecularprecision.com/wp-content/uploads/2023/04/QuantStudio-5-e1681219172931.png?fit=640%2C497&ssl=1" /> </div> </div> <div class="elementor-element elementor-element-29bafd6 elementor-widget elementor-widget-heading" data-id="29bafd6" data-element_type="widget" data-widget_type="heading.default"> <div class="elementor-widget-container"> <h2 class="elementor-heading-title elementor-size-default">QS5-DX™</h2> </div> </div> </div> <div class="elementor-element elementor-element-df74b76 e-con-full e-flex e-con e-child" data-id="df74b76" data-element_type="container"> <div class="elementor-element elementor-element-db4a52f elementor-widget elementor-widget-image" data-id="db4a52f" data-element_type="widget" data-widget_type="image.default"> <div class="elementor-widget-container"> <img loading="lazy" decoding="async" width="1536" height="1242" src="https://i0.wp.com/firalismolecularprecision.com/wp-content/uploads/2023/04/cfx-opus-deepwell.jpg?fit=1536%2C1242&ssl=1" class="attachment-1536x1536 size-1536x1536 wp-image-2190" alt="" data-attachment-id="2190" data-permalink="https://firalismolecularprecision.com/cro-cdmo-services/genomic-services/custom-rt-qpcr-services/cfx-opus-deepwell/" data-orig-file="https://i0.wp.com/firalismolecularprecision.com/wp-content/uploads/2023/04/cfx-opus-deepwell.jpg?fit=2864%2C2316&ssl=1" data-orig-size="2864,2316" data-comments-opened="1" data-image-meta="{"aperture":"14","credit":"","camera":"Canon EOS 6D Mark II","caption":"","created_timestamp":"1569642237","copyright":"","focal_length":"35","iso":"200","shutter_speed":"0.016666666666667","title":"","orientation":"1"}" data-image-title="cfx-opus-deepwell" data-image-description="" data-image-caption="" data-medium-file="https://i0.wp.com/firalismolecularprecision.com/wp-content/uploads/2023/04/cfx-opus-deepwell.jpg?fit=300%2C243&ssl=1" data-large-file="https://i0.wp.com/firalismolecularprecision.com/wp-content/uploads/2023/04/cfx-opus-deepwell.jpg?fit=640%2C518&ssl=1" /> </div> </div> <div class="elementor-element elementor-element-12846f2 elementor-widget elementor-widget-heading" data-id="12846f2" data-element_type="widget" data-widget_type="heading.default"> <div class="elementor-widget-container"> <h2 class="elementor-heading-title elementor-size-default">CFS Opus 96 - Dx</h2> </div> </div> </div> <div class="elementor-element elementor-element-4aed2ed e-con-full e-flex e-con e-child" data-id="4aed2ed" data-element_type="container"> <div class="elementor-element elementor-element-0b58323 elementor-widget elementor-widget-image" data-id="0b58323" data-element_type="widget" data-widget_type="image.default"> <div class="elementor-widget-container"> <img loading="lazy" decoding="async" width="150" height="150" src="https://i0.wp.com/firalismolecularprecision.com/wp-content/uploads/2023/04/ba49a.jpg?resize=150%2C150&ssl=1" class="attachment-thumbnail size-thumbnail wp-image-2191" alt="" data-attachment-id="2191" data-permalink="https://firalismolecularprecision.com/cro-cdmo-services/genomic-services/custom-rt-qpcr-services/ba49a/" data-orig-file="https://i0.wp.com/firalismolecularprecision.com/wp-content/uploads/2023/04/ba49a.jpg?fit=772%2C776&ssl=1" data-orig-size="772,776" data-comments-opened="1" data-image-meta="{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}" data-image-title="ba49a" data-image-description="" data-image-caption="" data-medium-file="https://i0.wp.com/firalismolecularprecision.com/wp-content/uploads/2023/04/ba49a.jpg?fit=298%2C300&ssl=1" data-large-file="https://i0.wp.com/firalismolecularprecision.com/wp-content/uploads/2023/04/ba49a.jpg?fit=640%2C643&ssl=1" /> </div> </div> <div class="elementor-element elementor-element-ff41c00 elementor-widget elementor-widget-heading" data-id="ff41c00" data-element_type="widget" data-widget_type="heading.default"> <div class="elementor-widget-container"> <h2 class="elementor-heading-title elementor-size-default">Fluidigm Biomark™ HD</h2> </div> </div> </div> </div> </div> <div class="elementor-element elementor-element-3e84600 e-flex e-con-boxed e-con e-parent" data-id="3e84600" data-element_type="container"> <div class="e-con-inner"> <div class="elementor-element elementor-element-5a79f19 e-con-full e-flex e-con e-child" data-id="5a79f19" data-element_type="container"> <div class="elementor-element elementor-element-f264189 elementor-widget-divider--view-line elementor-widget elementor-widget-divider" data-id="f264189" data-element_type="widget" data-widget_type="divider.default"> <div class="elementor-widget-container"> <div class="elementor-divider"> <span class="elementor-divider-separator"> </span> </div> </div> </div> </div> <div class="elementor-element elementor-element-88bb2fd e-con-full e-flex e-con e-child" data-id="88bb2fd" data-element_type="container"> <div class="elementor-element elementor-element-531d5f0 elementor-widget-divider--view-line elementor-widget elementor-widget-divider" data-id="531d5f0" data-element_type="widget" data-widget_type="divider.default"> <div class="elementor-widget-container"> <div class="elementor-divider"> <span class="elementor-divider-separator"> </span> </div> </div> </div> </div> <div class="elementor-element elementor-element-3fe8dbd e-con-full e-flex e-con e-child" data-id="3fe8dbd" data-element_type="container"> <div class="elementor-element elementor-element-efd27c5 elementor-widget-divider--view-line elementor-widget elementor-widget-divider" data-id="efd27c5" data-element_type="widget" data-widget_type="divider.default"> <div class="elementor-widget-container"> <div class="elementor-divider"> <span class="elementor-divider-separator"> </span> </div> </div> </div> </div> <div class="elementor-element elementor-element-3c407d1 e-con-full e-flex e-con e-child" data-id="3c407d1" data-element_type="container"> <div class="elementor-element elementor-element-bd86c7e elementor-widget-divider--view-line elementor-widget elementor-widget-divider" data-id="bd86c7e" data-element_type="widget" data-widget_type="divider.default"> <div class="elementor-widget-container"> <div class="elementor-divider"> <span class="elementor-divider-separator"> </span> </div> </div> </div> </div> <div class="elementor-element elementor-element-d8b14cd e-con-full e-flex e-con e-child" data-id="d8b14cd" data-element_type="container"> <div class="elementor-element elementor-element-933700c elementor-widget-divider--view-line elementor-widget elementor-widget-divider" data-id="933700c" data-element_type="widget" data-widget_type="divider.default"> <div class="elementor-widget-container"> <div class="elementor-divider"> <span class="elementor-divider-separator"> </span> </div> </div> </div> </div> </div> </div> <div class="elementor-element elementor-element-3f9dcb8 e-flex e-con-boxed e-con e-parent" data-id="3f9dcb8" data-element_type="container"> <div class="e-con-inner"> <div class="elementor-element elementor-element-5e7cecc e-con-full e-flex e-con e-child" data-id="5e7cecc" data-element_type="container"> <div class="elementor-element elementor-element-f9a09e9 elementor-widget__width-initial elementor-widget elementor-widget-text-editor" data-id="f9a09e9" data-element_type="widget" data-widget_type="text-editor.default"> <div class="elementor-widget-container"> <p>Number of samples per assay</p> </div> </div> </div> <div class="elementor-element elementor-element-df08eeb e-con-full e-flex e-con e-child" data-id="df08eeb" data-element_type="container"> <div class="elementor-element elementor-element-336fe07 elementor-widget elementor-widget-text-editor" data-id="336fe07" data-element_type="widget" data-widget_type="text-editor.default"> <div class="elementor-widget-container"> <p>96 samples/assay</p> </div> </div> </div> <div class="elementor-element elementor-element-f44f401 e-con-full e-flex e-con e-child" data-id="f44f401" data-element_type="container"> <div class="elementor-element elementor-element-76f39f8 elementor-widget elementor-widget-text-editor" data-id="76f39f8" data-element_type="widget" data-widget_type="text-editor.default"> <div class="elementor-widget-container"> <p>96 samples/assay</p> </div> </div> </div> <div class="elementor-element elementor-element-4311c57 e-con-full e-flex e-con e-child" data-id="4311c57" data-element_type="container"> <div class="elementor-element elementor-element-d95b7a0 elementor-widget elementor-widget-text-editor" data-id="d95b7a0" data-element_type="widget" data-widget_type="text-editor.default"> <div class="elementor-widget-container"> <p>96 samples/assay</p> </div> </div> </div> <div class="elementor-element elementor-element-9588e2f e-con-full e-flex e-con e-child" data-id="9588e2f" data-element_type="container"> <div class="elementor-element elementor-element-a7ebeec elementor-widget elementor-widget-text-editor" data-id="a7ebeec" data-element_type="widget" data-widget_type="text-editor.default"> <div class="elementor-widget-container"> <p>96 samples/assay</p> </div> </div> </div> </div> </div> <div class="elementor-element elementor-element-97a6a57 e-flex e-con-boxed e-con e-parent" data-id="97a6a57" data-element_type="container"> <div class="e-con-inner"> <div class="elementor-element elementor-element-2763b65 e-con-full e-flex e-con e-child" data-id="2763b65" data-element_type="container"> <div class="elementor-element elementor-element-31066d0 elementor-widget-divider--view-line elementor-widget elementor-widget-divider" data-id="31066d0" data-element_type="widget" data-widget_type="divider.default"> <div class="elementor-widget-container"> <div class="elementor-divider"> <span class="elementor-divider-separator"> </span> </div> </div> </div> </div> <div class="elementor-element elementor-element-12a8640 e-con-full e-flex e-con e-child" data-id="12a8640" data-element_type="container"> <div class="elementor-element elementor-element-3225dc2 elementor-widget-divider--view-line elementor-widget elementor-widget-divider" data-id="3225dc2" data-element_type="widget" data-widget_type="divider.default"> <div class="elementor-widget-container"> <div class="elementor-divider"> <span class="elementor-divider-separator"> </span> </div> </div> </div> </div> <div class="elementor-element elementor-element-8506fb4 e-con-full e-flex e-con e-child" data-id="8506fb4" data-element_type="container"> <div class="elementor-element elementor-element-9875425 elementor-widget-divider--view-line elementor-widget elementor-widget-divider" data-id="9875425" data-element_type="widget" data-widget_type="divider.default"> <div class="elementor-widget-container"> <div class="elementor-divider"> <span class="elementor-divider-separator"> </span> </div> </div> </div> </div> <div class="elementor-element elementor-element-64e450f e-con-full e-flex e-con e-child" data-id="64e450f" data-element_type="container"> <div class="elementor-element elementor-element-f3be401 elementor-widget-divider--view-line elementor-widget elementor-widget-divider" data-id="f3be401" data-element_type="widget" data-widget_type="divider.default"> <div class="elementor-widget-container"> <div class="elementor-divider"> <span class="elementor-divider-separator"> </span> </div> </div> </div> </div> <div class="elementor-element elementor-element-7311c7c e-con-full e-flex e-con e-child" data-id="7311c7c" data-element_type="container"> <div class="elementor-element elementor-element-a431284 elementor-widget-divider--view-line elementor-widget elementor-widget-divider" data-id="a431284" data-element_type="widget" data-widget_type="divider.default"> <div class="elementor-widget-container"> <div class="elementor-divider"> <span class="elementor-divider-separator"> </span> </div> </div> </div> </div> </div> </div> <div class="elementor-element elementor-element-171b5f9 e-flex e-con-boxed e-con e-parent" data-id="171b5f9" data-element_type="container"> <div class="e-con-inner"> <div class="elementor-element elementor-element-7d299d3 e-con-full e-flex e-con e-child" data-id="7d299d3" data-element_type="container"> <div class="elementor-element elementor-element-c100f75 elementor-widget__width-initial elementor-widget elementor-widget-text-editor" data-id="c100f75" data-element_type="widget" data-widget_type="text-editor.default"> <div class="elementor-widget-container"> <p>RUO</p> </div> </div> </div> <div class="elementor-element elementor-element-5d9525c e-con-full e-flex e-con e-child" data-id="5d9525c" data-element_type="container"> <div class="elementor-element elementor-element-2e47505 elementor-view-default elementor-widget elementor-widget-icon" data-id="2e47505" data-element_type="widget" data-widget_type="icon.default"> <div class="elementor-widget-container"> <div class="elementor-icon-wrapper"> <div class="elementor-icon"> <svg aria-hidden="true" class="e-font-icon-svg e-fas-check" viewBox="0 0 512 512" xmlns="http://www.w3.org/2000/svg"><path d="M173.898 439.404l-166.4-166.4c-9.997-9.997-9.997-26.206 0-36.204l36.203-36.204c9.997-9.998 26.207-9.998 36.204 0L192 312.69 432.095 72.596c9.997-9.997 26.207-9.997 36.204 0l36.203 36.204c9.997 9.997 9.997 26.206 0 36.204l-294.4 294.401c-9.998 9.997-26.207 9.997-36.204-.001z"></path></svg> </div> </div> </div> </div> </div> <div class="elementor-element elementor-element-7da6d65 e-con-full e-flex e-con e-child" data-id="7da6d65" data-element_type="container"> <div class="elementor-element elementor-element-504eeb5 elementor-view-default elementor-widget elementor-widget-icon" data-id="504eeb5" data-element_type="widget" data-widget_type="icon.default"> <div class="elementor-widget-container"> <div class="elementor-icon-wrapper"> <div class="elementor-icon"> <svg aria-hidden="true" class="e-font-icon-svg e-fas-check" viewBox="0 0 512 512" xmlns="http://www.w3.org/2000/svg"><path d="M173.898 439.404l-166.4-166.4c-9.997-9.997-9.997-26.206 0-36.204l36.203-36.204c9.997-9.998 26.207-9.998 36.204 0L192 312.69 432.095 72.596c9.997-9.997 26.207-9.997 36.204 0l36.203 36.204c9.997 9.997 9.997 26.206 0 36.204l-294.4 294.401c-9.998 9.997-26.207 9.997-36.204-.001z"></path></svg> </div> </div> </div> </div> </div> <div class="elementor-element elementor-element-b79afd3 e-con-full e-flex e-con e-child" data-id="b79afd3" data-element_type="container"> <div class="elementor-element elementor-element-a4b61d4 elementor-view-default elementor-widget elementor-widget-icon" data-id="a4b61d4" data-element_type="widget" data-widget_type="icon.default"> <div class="elementor-widget-container"> <div class="elementor-icon-wrapper"> <div class="elementor-icon"> <svg aria-hidden="true" class="e-font-icon-svg e-fas-check" viewBox="0 0 512 512" xmlns="http://www.w3.org/2000/svg"><path d="M173.898 439.404l-166.4-166.4c-9.997-9.997-9.997-26.206 0-36.204l36.203-36.204c9.997-9.998 26.207-9.998 36.204 0L192 312.69 432.095 72.596c9.997-9.997 26.207-9.997 36.204 0l36.203 36.204c9.997 9.997 9.997 26.206 0 36.204l-294.4 294.401c-9.998 9.997-26.207 9.997-36.204-.001z"></path></svg> </div> </div> </div> </div> </div> <div class="elementor-element elementor-element-84d1a81 e-con-full e-flex e-con e-child" data-id="84d1a81" data-element_type="container"> <div class="elementor-element elementor-element-0be48fd elementor-view-default elementor-widget elementor-widget-icon" data-id="0be48fd" data-element_type="widget" data-widget_type="icon.default"> <div class="elementor-widget-container"> <div class="elementor-icon-wrapper"> <div class="elementor-icon"> <svg aria-hidden="true" class="e-font-icon-svg e-fas-check" viewBox="0 0 512 512" xmlns="http://www.w3.org/2000/svg"><path d="M173.898 439.404l-166.4-166.4c-9.997-9.997-9.997-26.206 0-36.204l36.203-36.204c9.997-9.998 26.207-9.998 36.204 0L192 312.69 432.095 72.596c9.997-9.997 26.207-9.997 36.204 0l36.203 36.204c9.997 9.997 9.997 26.206 0 36.204l-294.4 294.401c-9.998 9.997-26.207 9.997-36.204-.001z"></path></svg> </div> </div> </div> </div> </div> </div> </div> <div class="elementor-element elementor-element-c8dd6c8 e-flex e-con-boxed e-con e-parent" data-id="c8dd6c8" data-element_type="container"> <div class="e-con-inner"> <div class="elementor-element elementor-element-d819633 e-con-full e-flex e-con e-child" data-id="d819633" data-element_type="container"> <div class="elementor-element elementor-element-f65bbfc elementor-widget-divider--view-line elementor-widget elementor-widget-divider" data-id="f65bbfc" data-element_type="widget" data-widget_type="divider.default"> <div class="elementor-widget-container"> <div class="elementor-divider"> <span class="elementor-divider-separator"> </span> </div> </div> </div> </div> <div class="elementor-element elementor-element-d73541f e-con-full e-flex e-con e-child" data-id="d73541f" data-element_type="container"> <div class="elementor-element elementor-element-d1979be elementor-widget-divider--view-line elementor-widget elementor-widget-divider" data-id="d1979be" data-element_type="widget" data-widget_type="divider.default"> <div class="elementor-widget-container"> <div class="elementor-divider"> <span class="elementor-divider-separator"> </span> </div> </div> </div> </div> <div class="elementor-element elementor-element-650788c e-con-full e-flex e-con e-child" data-id="650788c" data-element_type="container"> <div class="elementor-element elementor-element-0e4b017 elementor-widget-divider--view-line elementor-widget elementor-widget-divider" data-id="0e4b017" data-element_type="widget" data-widget_type="divider.default"> <div class="elementor-widget-container"> <div class="elementor-divider"> <span class="elementor-divider-separator"> </span> </div> </div> </div> </div> <div class="elementor-element elementor-element-480a404 e-con-full e-flex e-con e-child" data-id="480a404" data-element_type="container"> <div class="elementor-element elementor-element-5a199e3 elementor-widget-divider--view-line elementor-widget elementor-widget-divider" data-id="5a199e3" data-element_type="widget" data-widget_type="divider.default"> <div class="elementor-widget-container"> <div class="elementor-divider"> <span class="elementor-divider-separator"> </span> </div> </div> </div> </div> <div class="elementor-element elementor-element-5f48fc9 e-con-full e-flex e-con e-child" data-id="5f48fc9" data-element_type="container"> <div class="elementor-element elementor-element-8f946f1 elementor-widget-divider--view-line elementor-widget elementor-widget-divider" data-id="8f946f1" data-element_type="widget" data-widget_type="divider.default"> <div class="elementor-widget-container"> <div class="elementor-divider"> <span class="elementor-divider-separator"> </span> </div> </div> </div> </div> </div> </div> <div class="elementor-element elementor-element-8121302 e-flex e-con-boxed e-con e-parent" data-id="8121302" data-element_type="container"> <div class="e-con-inner"> <div class="elementor-element elementor-element-53a3c47 e-con-full e-flex e-con e-child" data-id="53a3c47" data-element_type="container"> <div class="elementor-element elementor-element-f462eac elementor-widget__width-initial elementor-widget elementor-widget-text-editor" data-id="f462eac" data-element_type="widget" data-widget_type="text-editor.default"> <div class="elementor-widget-container"> <p>IVDR</p> </div> </div> </div> <div class="elementor-element elementor-element-437cd0e e-con-full e-flex e-con e-child" data-id="437cd0e" data-element_type="container"> <div class="elementor-element elementor-element-2110a47 elementor-view-default elementor-widget elementor-widget-icon" data-id="2110a47" data-element_type="widget" data-widget_type="icon.default"> <div class="elementor-widget-container"> <div class="elementor-icon-wrapper"> <div class="elementor-icon"> <svg aria-hidden="true" class="e-font-icon-svg e-fas-times" viewBox="0 0 352 512" xmlns="http://www.w3.org/2000/svg"><path d="M242.72 256l100.07-100.07c12.28-12.28 12.28-32.19 0-44.48l-22.24-22.24c-12.28-12.28-32.19-12.28-44.48 0L176 189.28 75.93 89.21c-12.28-12.28-32.19-12.28-44.48 0L9.21 111.45c-12.28 12.28-12.28 32.19 0 44.48L109.28 256 9.21 356.07c-12.28 12.28-12.28 32.19 0 44.48l22.24 22.24c12.28 12.28 32.2 12.28 44.48 0L176 322.72l100.07 100.07c12.28 12.28 32.2 12.28 44.48 0l22.24-22.24c12.28-12.28 12.28-32.19 0-44.48L242.72 256z"></path></svg> </div> </div> </div> </div> </div> <div class="elementor-element elementor-element-4fd3967 e-con-full e-flex 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data-widget_type="text-editor.default"> <div class="elementor-widget-container"> <p>Manufacturer’s information</p> </div> </div> </div> <div class="elementor-element elementor-element-88da299 e-con-full e-flex e-con e-child" data-id="88da299" data-element_type="container"> <div class="elementor-element elementor-element-1fdf938 elementor-align-center elementor-widget elementor-widget-button" data-id="1fdf938" data-element_type="widget" data-widget_type="button.default"> <div class="elementor-widget-container"> <div class="elementor-button-wrapper"> <a class="elementor-button elementor-button-link elementor-size-sm elementor-animation-grow" href="https://www.bio-rad.com/fr-fr/product/cfx96-touch-real-time-pcr-detection-system?ID=LJB1YU15" target="_blank"> <span class="elementor-button-content-wrapper"> <span class="elementor-button-icon"> <svg aria-hidden="true" class="e-font-icon-svg e-far-file-alt" viewBox="0 0 384 512" xmlns="http://www.w3.org/2000/svg"><path d="M288 248v28c0 6.6-5.4 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elementor-size-sm elementor-animation-grow" href="https://www.thermofisher.com/fr/fr/home/life-science/pcr/real-time-pcr/real-time-pcr-instruments/quantstudio-systems/models/quantstudio-3-5.html" target="_blank"> <span class="elementor-button-content-wrapper"> <span class="elementor-button-icon"> <svg aria-hidden="true" class="e-font-icon-svg e-far-file-alt" viewBox="0 0 384 512" xmlns="http://www.w3.org/2000/svg"><path d="M288 248v28c0 6.6-5.4 12-12 12H108c-6.6 0-12-5.4-12-12v-28c0-6.6 5.4-12 12-12h168c6.6 0 12 5.4 12 12zm-12 72H108c-6.6 0-12 5.4-12 12v28c0 6.6 5.4 12 12 12h168c6.6 0 12-5.4 12-12v-28c0-6.6-5.4-12-12-12zm108-188.1V464c0 26.5-21.5 48-48 48H48c-26.5 0-48-21.5-48-48V48C0 21.5 21.5 0 48 0h204.1C264.8 0 277 5.1 286 14.1L369.9 98c9 8.9 14.1 21.2 14.1 33.9zm-128-80V128h76.1L256 51.9zM336 464V176H232c-13.3 0-24-10.7-24-24V48H48v416h288z"></path></svg> </span> <span class="elementor-button-text">Know more</span> </span> </a> </div> </div> </div> </div> <div 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class="elementor-button-wrapper"> <a class="elementor-button elementor-button-link elementor-size-sm elementor-animation-grow" href="https://www.gene-quantification.de/flyer-biomark-96x96.pdf" target="_blank"> <span class="elementor-button-content-wrapper"> <span class="elementor-button-icon"> <svg aria-hidden="true" class="e-font-icon-svg e-far-file-alt" viewBox="0 0 384 512" xmlns="http://www.w3.org/2000/svg"><path d="M288 248v28c0 6.6-5.4 12-12 12H108c-6.6 0-12-5.4-12-12v-28c0-6.6 5.4-12 12-12h168c6.6 0 12 5.4 12 12zm-12 72H108c-6.6 0-12 5.4-12 12v28c0 6.6 5.4 12 12 12h168c6.6 0 12-5.4 12-12v-28c0-6.6-5.4-12-12-12zm108-188.1V464c0 26.5-21.5 48-48 48H48c-26.5 0-48-21.5-48-48V48C0 21.5 21.5 0 48 0h204.1C264.8 0 277 5.1 286 14.1L369.9 98c9 8.9 14.1 21.2 14.1 33.9zm-128-80V128h76.1L256 51.9zM336 464V176H232c-13.3 0-24-10.7-24-24V48H48v416h288z"></path></svg> </span> <span class="elementor-button-text">Know more</span> </span> </a> </div> </div> </div> </div> </div> </div> <div class="elementor-element elementor-element-d8294c8 e-flex e-con-boxed e-con e-parent" data-id="d8294c8" data-element_type="container"> <div class="e-con-inner"> <div class="elementor-element elementor-element-7d9f799 elementor-widget elementor-widget-heading" data-id="7d9f799" data-element_type="widget" data-widget_type="heading.default"> <div class="elementor-widget-container"> <h2 class="elementor-heading-title elementor-size-default">real-time pcr (qPCR) principle</h2> </div> </div> <div class="elementor-element elementor-element-dab6275 elementor-widget-divider--view-line elementor-widget elementor-widget-divider" data-id="dab6275" data-element_type="widget" data-widget_type="divider.default"> <div class="elementor-widget-container"> <div class="elementor-divider"> <span class="elementor-divider-separator"> </span> </div> </div> </div> <div class="elementor-element elementor-element-0be35dc animated-slow elementor-invisible elementor-widget elementor-widget-text-editor" data-id="0be35dc" data-element_type="widget" data-settings="{"_animation":"fadeIn","_animation_delay":300}" data-widget_type="text-editor.default"> <div class="elementor-widget-container"> <p>The principle of qPCR lies in the continuous monitoring of DNA amplification during the reaction, achieved through the use of fluorescent dyes or probes.</p><p><span style="color: #008000;"><strong>Amplification</strong>:</span><br />Like conventional PCR, qPCR involves repeated cycles of denaturation, annealing, and extension to amplify a target DNA sequence. If the starting material is RNA, a reverse transcription step is performed first to generate complementary DNA (cDNA).</p><p><span style="color: #008000;"><strong>Fluorescence Detection</strong>:</span></p><p style="padding-left: 40px;"><strong>• </strong><span style="text-decoration: underline;">Intercalating Dyes</span><span style="text-decoration: underline;">:</span> Dyes such as SYBR Green bind to double-stranded DNA and emit fluorescence proportional to the amount of DNA generated.</p><p style="padding-left: 40px;"><strong>•</strong> <span style="text-decoration: underline;">Probe-Based Detection:</span> Probes like TaqMan contain a fluorescent reporter and quencher, providing target-specific fluorescence upon successful amplification.</p><p><span style="color: #008000;"><strong>Real-Time Quantification</strong>:</span><br />The fluorescence intensity is measured during each cycle, allowing the accumulation of DNA to be tracked in real time. The cycle at which fluorescence surpasses a set threshold (Ct value) reflects the initial quantity of the target DNA.</p><p style="text-align: left;"><span style="color: #008000;"><strong>Quantification Methods</strong>:</span><br />qPCR can provide absolute quantification using standard curves, or relative quantification (comparing target expression to reference genes).</p> </div> </div> </div> </div> <div class="elementor-element elementor-element-27e918b e-flex e-con-boxed e-con e-parent" data-id="27e918b" data-element_type="container" data-settings="{"background_background":"classic"}"> <div class="e-con-inner"> <div class="elementor-element elementor-element-7d0aa7c elementor-widget elementor-widget-heading" data-id="7d0aa7c" data-element_type="widget" data-widget_type="heading.default"> <div class="elementor-widget-container"> <h2 class="elementor-heading-title elementor-size-default">For more details on each system and its specific applications, connect with our experts today! We're here to help you find the perfect solution for your research or diagnostic needs.</h2> </div> </div> <div class="elementor-element elementor-element-a4d317f elementor-button-info elementor-align-center elementor-widget elementor-widget-button" data-id="a4d317f" data-element_type="widget" data-widget_type="button.default"> <div class="elementor-widget-container"> <div class="elementor-button-wrapper"> <a class="elementor-button elementor-button-link elementor-size-lg" href="https://firalismolecularprecision.com/contact-us/" target="_blank"> <span class="elementor-button-content-wrapper"> <span class="elementor-button-text">Talk to one of our experts</span> </span> </a> </div> </div> </div> </div> </div> <div class="elementor-element elementor-element-36b5cba e-flex e-con-boxed e-con e-parent" data-id="36b5cba" data-element_type="container"> <div class="e-con-inner"> <div class="elementor-element elementor-element-3bd30a6 e-con-full e-flex e-con e-child" data-id="3bd30a6" 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447.9 17.9 449 8.4 441.6.1 432 .1L399.6 0c-8.1 0-14.9 5.9-16 13.9-.7 4.9-1.8 11.1-3.4 18.1H67.8c-1.6-7-2.7-13.1-3.4-18.1-1.1-8-7.9-14-16-13.9L16.1.1C6.5.1-1 8.4.1 17.9 5.3 60.8 31.4 171.8 160 256 31.5 340.2 5.3 451.2.1 494.1zM224 219.6c-25.1-13.7-46.4-28.4-64.3-43.6h128.5c-17.8 15.2-39.1 30-64.2 43.6zM355.1 96c-5.8 10.4-12.8 21.1-21 32H114c-8.3-10.9-15.3-21.6-21-32h262.1zM92.9 416c5.8-10.4 12.8-21.1 21-32h219.4c8.3 10.9 15.4 21.6 21.2 32H92.9z"></path></svg> </div> </div> </div> </div> <div class="elementor-element elementor-element-a149c73 elementor-widget elementor-widget-heading" data-id="a149c73" data-element_type="widget" data-widget_type="heading.default"> <div class="elementor-widget-container"> <h2 class="elementor-heading-title elementor-size-default">Complete workflow</h2> </div> </div> <div class="elementor-element elementor-element-f420494 elementor-widget elementor-widget-text-editor" data-id="f420494" data-element_type="widget" data-widget_type="text-editor.default"> <div class="elementor-widget-container"> <p>Our qPCR service covers the entire workflow, <strong>from the initial design of primers to the final analysis of PCR data.</strong> We collaborate closely with you to understand your specific needs and make any necessary adjustments to ensure optimal results.</p> </div> </div> </div> <div class="elementor-element elementor-element-711c864 e-con-full animated-slow e-flex elementor-invisible e-con e-child" data-id="711c864" data-element_type="container" data-settings="{"animation":"fadeIn","animation_delay":600}"> <div class="elementor-element elementor-element-ef42076 elementor-view-default elementor-widget elementor-widget-icon" data-id="ef42076" data-element_type="widget" data-widget_type="icon.default"> <div class="elementor-widget-container"> <div class="elementor-icon-wrapper"> <div class="elementor-icon"> <svg aria-hidden="true" class="e-font-icon-svg e-far-user" viewBox="0 0 448 512" xmlns="http://www.w3.org/2000/svg"><path d="M313.6 304c-28.7 0-42.5 16-89.6 16-47.1 0-60.8-16-89.6-16C60.2 304 0 364.2 0 438.4V464c0 26.5 21.5 48 48 48h352c26.5 0 48-21.5 48-48v-25.6c0-74.2-60.2-134.4-134.4-134.4zM400 464H48v-25.6c0-47.6 38.8-86.4 86.4-86.4 14.6 0 38.3 16 89.6 16 51.7 0 74.9-16 89.6-16 47.6 0 86.4 38.8 86.4 86.4V464zM224 288c79.5 0 144-64.5 144-144S303.5 0 224 0 80 64.5 80 144s64.5 144 144 144zm0-240c52.9 0 96 43.1 96 96s-43.1 96-96 96-96-43.1-96-96 43.1-96 96-96z"></path></svg> </div> </div> </div> </div> <div class="elementor-element elementor-element-1ec942e elementor-widget elementor-widget-heading" data-id="1ec942e" data-element_type="widget" data-widget_type="heading.default"> <div class="elementor-widget-container"> <h2 class="elementor-heading-title elementor-size-default">Qualified Team</h2> </div> </div> <div class="elementor-element elementor-element-226fe43 elementor-widget elementor-widget-text-editor" data-id="226fe43" data-element_type="widget" data-widget_type="text-editor.default"> <div 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We provide comprehensive support throughout the entire process, from sample preparation to data analysis, ensuring a successful project outcome.</p> </div> </div> </div> <div class="elementor-element elementor-element-6efa707 e-con-full animated-slow e-flex elementor-invisible e-con e-child" data-id="6efa707" data-element_type="container" data-settings="{"animation":"fadeIn","animation_delay":400}"> <div class="elementor-element elementor-element-53eb7e6 elementor-view-default elementor-widget elementor-widget-icon" data-id="53eb7e6" data-element_type="widget" data-widget_type="icon.default"> <div class="elementor-widget-container"> <div class="elementor-icon-wrapper"> <div class="elementor-icon"> <svg aria-hidden="true" class="e-font-icon-svg e-far-chart-bar" viewBox="0 0 512 512" xmlns="http://www.w3.org/2000/svg"><path d="M396.8 352h22.4c6.4 0 12.8-6.4 12.8-12.8V108.8c0-6.4-6.4-12.8-12.8-12.8h-22.4c-6.4 0-12.8 6.4-12.8 12.8v230.4c0 6.4 6.4 12.8 12.8 12.8zm-192 0h22.4c6.4 0 12.8-6.4 12.8-12.8V140.8c0-6.4-6.4-12.8-12.8-12.8h-22.4c-6.4 0-12.8 6.4-12.8 12.8v198.4c0 6.4 6.4 12.8 12.8 12.8zm96 0h22.4c6.4 0 12.8-6.4 12.8-12.8V204.8c0-6.4-6.4-12.8-12.8-12.8h-22.4c-6.4 0-12.8 6.4-12.8 12.8v134.4c0 6.4 6.4 12.8 12.8 12.8zM496 400H48V80c0-8.84-7.16-16-16-16H16C7.16 64 0 71.16 0 80v336c0 17.67 14.33 32 32 32h464c8.84 0 16-7.16 16-16v-16c0-8.84-7.16-16-16-16zm-387.2-48h22.4c6.4 0 12.8-6.4 12.8-12.8v-70.4c0-6.4-6.4-12.8-12.8-12.8h-22.4c-6.4 0-12.8 6.4-12.8 12.8v70.4c0 6.4 6.4 12.8 12.8 12.8z"></path></svg> </div> </div> </div> </div> <div class="elementor-element elementor-element-b925dcb elementor-widget elementor-widget-heading" data-id="b925dcb" data-element_type="widget" data-widget_type="heading.default"> <div class="elementor-widget-container"> <h2 class="elementor-heading-title elementor-size-default">High-quality results</h2> </div> </div> <div class="elementor-element elementor-element-6a5de18 elementor-widget elementor-widget-text-editor" data-id="6a5de18" data-element_type="widget" data-widget_type="text-editor.default"> <div class="elementor-widget-container"> <p>Our experts use advanced platforms to conduct qPCR experiments with exceptional sensitivity and specificity, delivering high-quality data. 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