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href="https://www.quantum-si.com/about-old__trashed/customer-connections/">Customer Connections</a> </li> <li class="menu-item menu-item-type-custom menu-item-object-custom "> <a href="https://ir.quantum-si.com/">Investors</a> </li> <li class="menu-item menu-item-type-post_type menu-item-object-page "> <a href="https://www.quantum-si.com/distributor/">Distributors</a> </li> </ul> </li> <li class="menu-item menu-item-type-post_type menu-item-object-page "> <a target="_self" href="https://www.quantum-si.com/contact/">Contact</a> </li> </ul> </nav> <button class="hamburger hamburger--slider" type="button"> <span class="hamburger-box"> <span class="hamburger-inner"></span> </span> </button> </div> </div> </header> <section id="content" role="main" class="content-wrapper"> <div class="wrapper "> <section id="hero" data-bg="https://www.quantum-si.com/wp-content/uploads/ptm-hero.jpg"> <div class="row align-right" > <div class="column large-6"> <h1>Uncover New Insights with <highlight>Post-translational Modification</highlight> (PTM) Analysis</h1> <p>Interrogate PTMs and proteoforms with ease on Platinum’s® Next-Generation Protein Sequencing™ platform.</p> <a href="https://www.quantum-si.com/resources/app-notes/detecting-arginine-post-translational-modifications-using-quantum-si-next-generation-protein-sequencing-technology/" class="button" target="">Download App Note</a> </div> </div> </section> <section class="text-image" data-block_df739258b7dcf0ff09865e55eeeba821 id="text-image-generate-deeper-insights"> <div class="row medium-unstack align-middle "> <div class="column"> <span class="applications-header"> <a href="https://www.quantum-si.com/applications/">Applications</a> <span class="arrow">&rsaquo;</span> Post Translational Modification Analysis </span> <h2> Generate Deeper Insights </h2> <p> <p>Discover more about your proteins! Platinum® technology enables you to discover new insights into biological function with the capability to conduct analysis of post-translational modifications (PTMs).</p> </p> </div> </div> </section> <section class="accordion-section" data-block_75c1e5121ec4a4abb5da4a14639cc98f> <div class="row"> <div class="column"> <h3>See how Platinum’s single-molecule resolution overcomes obstacles presented by other PTM analysis methods:</h3> <ul class="accordion" data-accordion data-allow-all-closed="true"> <li class="accordion-item" data-accordion-item> <a href="#" class="accordion-title">Integrating Top-Down Mass Spectrometry and Single-Molecule Sequencing for Proteoform Analysis — WEBINAR <div class="webinar-info"> <span class="speaker-name">Dr. Michael Caldwell,Scientific Officer at Northwestern Proteomics Center of Excellence, Dr. Meredith Carpenter, Head of Scientific Affairs, QSI and Dr. Kenneth Skinner, Senior Scientific Liaison, QSI</span> <span class="webinar-date">September 30, 2024</span> </div></a> <div class="accordion-content" data-tab-content> <p>Given the numerous sources of protein variation, ranging from biological factors to sample preparation and instrument settings, there is a pressing need for next-generation analytical platforms capable of driving proteoform analysis with complete molecular precision.</p> <p>In this webinar, Dr. Michael Caldwell, Scientific Officer at Northwestern Proteomics Center of Excellence, explores top-down mass spectrometry (TD-MS) and Platinum<sup>®</sup>.</p> <p>Platinum is a benchtop instrument that provides single amino acid resolution of single protein molecules to characterize IL-6, including pyroglutamate (pE), a post-translational modification (PTM) produced via enzymatic and spontaneous cyclization of N-terminal glutamine (Q)/glutamate (E) residues.</p> <p>Find out how TD-MS enables intact protein analysis, limiting formation of N-terminal Q/E (MS1). During MS2, analysis of daughter ions reveals modifications and artifactual formation. Platinum employs dye-labeled N-terminal amino acid recognizers that reveal primary structure and variants, and aminopeptidases that expose the next amino acid for recognition until the peptide is sequenced. TD-MS and Platinum detect not only functionally relevant regions of IL-6 primary structure, but also sites prone to pE formation.</p> <p><strong>Key Learning Points/Takeaways:</strong></p> <ul class="wp-block-list"> <li>Understand how top-down mass spectrometry (TD-MS) and Next-Generation Protein Sequencing can complement each other for comprehensive protein analysis.</li> <li>Explore methods to bridge discovery and targeted protein analysis across different instruments and detection methods.</li> <li>Learn how TD-MS distinguishes between native and artifactual proteoforms.</li> </ul> <div class="key-learning"></div> <p><strong>Speakers:</strong> Michael Caldwell, PhD – Northwestern University, Meredith Carpenter, PhD – QSI and Kenneth Skinner, PhD – QSI</p> <p><strong>Date:</strong> September 30, 2024</p> <p><a class="wp-block-button__link has-black-color has-text-color wp-element-button" href="/resources/webinars/integrating-top-down-mass-spectrometry-and-single-molecule-sequencing-for-proteoform-analysis/">Watch Now</a></p> </div> </li> <li class="accordion-item" data-accordion-item> <a href="#" class="accordion-title">Detection and Differentiation of PTMs with Platinum – APP NOTE</a> <div class="accordion-content" data-tab-content> <p><strong>Arginine PTM analysis</strong></p> <p>Arginine plays a critical role in protein structure and function and often mediates key interactions between protein binding partners or between proteins and DNA. The two most common arginine PTMs are citrullination and dimethylation, which respectively alter arginine into citrulline or ADMA and SDMA.</p> <figure><img fetchpriority="high" decoding="async" class="wp-image-306" src="https://www.quantum-si.com/wp-content/uploads/2023/04/The-limitations-of-arginine-PTM-analysis-1024x524.webp" sizes="(max-width: 1024px) 100vw, 1024px" srcset="https://www.quantum-si.com/wp-content/uploads/2023/04/The-limitations-of-arginine-PTM-analysis-1024x524.webp 1024w, https://www.quantum-si.com/wp-content/uploads/2023/04/The-limitations-of-arginine-PTM-analysis-300x154.webp 300w, https://www.quantum-si.com/wp-content/uploads/2023/04/The-limitations-of-arginine-PTM-analysis-768x393.webp 768w, https://www.quantum-si.com/wp-content/uploads/2023/04/The-limitations-of-arginine-PTM-analysis.webp 1190w" alt="" width="1024" height="524" /></figure> <p>ADMA and SDMA isomers share structural similarities and an identical mass, limiting their identification through traditional mass spectrometry. Likewise, determining arginine to citrulline results in a negligible mass increase. Another common method for PTM detection is antibody-based immunoassay ELISA, but only a few antibodies against arginine PTMs are commercially available, restricting its application.</p> <p><strong>Direct detection of arginine PTMs in proteins with Platinum</strong></p> <p>With Platinum’s single-molecule resolution and sensitivity, you can detect and differentiate arginine PTMs accurately. Peptide fragments are probed in real-time by fluorescently labeled recognizers, designed to bind on and off to their cognate N-terminal amino acid (NAA), while aminopeptidases in solution remove analyzed NAAs to expose subsequent amino acids for further recognition.<br /> Fluorescence lifetime, intensity, and kinetic data are collected in real-time and analyzed to determine amino acid sequence and PTM content. This novel single-molecule sequencing approach can overcome the challenges facing conventional methods through unparalleled amino acid detection.</p> <p>Using Quantum-Si’s single-molecule protein sequencing, we demonstrated how to detect citrulline and how to differentiate between dimethylated arginine residues efficiently.</p> <p><a class="wp-block-button__link has-black-color has-text-color wp-element-button" href="https://www.quantum-si.com/resources/app-notes/detecting-arginine-post-translational-modifications-using-quantum-si-next-generation-protein-sequencing-technology/">See the Data</a></p> <figure><img decoding="async" class="wp-image-306" src="https://www.quantum-si.com/wp-content/uploads/Detecting_Arginine_PTMs_Fig3.jpg" sizes="(max-width: 1024px) 100vw, 1024px" />Platinum Can Detect and Differentiate PTMs – <a href="https://www.quantum-si.com/resources/app-notes/detecting-arginine-post-translational-modifications-using-quantum-si-next-generation-protein-sequencing-technology/">Download the application note</a> to see how the process works and the PTM analysis that Platinum® delivers.</figure> </div> </li> <li class="accordion-item" data-accordion-item> <a href="#" class="accordion-title">Beyond the Genome: Identification of Protein Sequence and PTMs with Quantum-Si’s Next- Generation Protein Sequencer™ Platinum® – POSTER</a> <div class="accordion-content" data-tab-content> <p>To demonstrate the versatility of Platinum and the use of kinetic signatures, we sequenced various types of samples, including mixtures of recombinant proteins, peptides with post-translational modifications (PTMs), proteins immunoprecipitated from human serum, and proteins isolated from human serum via fractionation with SDS-PAGE. The resulting peptides generated distinct kinetic signatures aligned to their respective sequences, highlighting the efficacy of Quantum-Si’s sequencing platform in analyzing multi-protein mixtures at reduced input concentrations.</p> <p><a class="wp-block-button__link has-black-color has-text-color wp-element-button" href="/resources/posters/beyond-the-genome-identification-of-protein-sequence-and-ptms-with-quantum-sis-next-generation-protein-sequencer-platinum/">Download Poster</a></p> </div> </li> <li class="accordion-item" data-accordion-item> <a href="#" class="accordion-title">Beyond the Genome: Unraveling Protein Variability with Quantum-Si’s Next-Generation Protein Sequencing™ Technology – POSTER</a> <div class="accordion-content" data-tab-content> <p>Protein sequencing is a groundbreaking advancement in proteomics that augments genomics and transcriptomics research by providing crucial insights into the functional proteins encoded by the genome. Protein sequencing offers a more complete understanding of cellular processes and disease mechanisms by detecting changes at the protein level, such as post-translational modifications (PTMs), which cannot be captured by genomics data alone. Next-Generation Protein Sequencing™ (NGPS) on Platinum® enables researchers to identify and characterize proteins with single-molecule resolution in a simple workflow and on a benchtop instrument.</p> <p><a class="wp-block-button__link has-black-color has-text-color wp-element-button" href="/resources/posters/beyond-the-genome-unraveling-protein-variability-with-quantum-sis-next-generation-protein-sequencing-technology-2/">Download Poster</a></p> </div> </li> </ul> </div> </div> </section> </div> </section> <div class="footer-bar"><p>RESEARCH USE ONLY. 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