CINXE.COM

PBMC Isolation and Cryopreservation Tech Note | GENEWIZ from Azenta

<!doctype html><html lang="en"><head> <meta charset="utf-8"> <title>PBMC Isolation and Cryopreservation Tech Note | GENEWIZ from Azenta</title> <link rel="shortcut icon" href="https://web.genewiz.com/hubfs/GENEWIZ-Favicon_PublicWeb_40x40@3x.png"> <meta name="description" content="Learn about our optimized workflows for the rapid isolation and freezing of PBMCs with reproducible, best-in-class results. Download tech note."> <meta name="viewport" content="width=device-width, initial-scale=1"> <script src="/hs/hsstatic/jquery-libs/static-1.1/jquery/jquery-1.7.1.js"></script> <script>hsjQuery = window['jQuery'];</script> <meta property="og:description" content="Learn about our optimized workflows for the rapid isolation and freezing of PBMCs with reproducible, best-in-class results. Download tech note."> <meta property="og:title" content="PBMC Isolation and Cryopreservation Tech Note | GENEWIZ from Azenta"> <meta name="twitter:description" content="Learn about our optimized workflows for the rapid isolation and freezing of PBMCs with reproducible, best-in-class results. Download tech note."> <meta name="twitter:title" content="PBMC Isolation and Cryopreservation Tech Note | GENEWIZ from Azenta"> <style> a.cta_button{-moz-box-sizing:content-box !important;-webkit-box-sizing:content-box !important;box-sizing:content-box !important;vertical-align:middle}.hs-breadcrumb-menu{list-style-type:none;margin:0px 0px 0px 0px;padding:0px 0px 0px 0px}.hs-breadcrumb-menu-item{float:left;padding:10px 0px 10px 10px}.hs-breadcrumb-menu-divider:before{content:'›';padding-left:10px}.hs-featured-image-link{border:0}.hs-featured-image{float:right;margin:0 0 20px 20px;max-width:50%}@media (max-width: 568px){.hs-featured-image{float:none;margin:0;width:100%;max-width:100%}}.hs-screen-reader-text{clip:rect(1px, 1px, 1px, 1px);height:1px;overflow:hidden;position:absolute !important;width:1px} </style> <link rel="stylesheet" href="https://web.genewiz.com/hs-fs/hub/3478602/hub_generated/template_assets/69476735040/1721068281488/Azenta_Templates/Form_Conversion_LP_Template/css/main.min.css"> <link rel="stylesheet" href="https://cdnjs.cloudflare.com/ajax/libs/font-awesome/5.15.1/css/all.min.css"> <link rel="stylesheet" href="https://cdn2.hubspot.net/hub/-1/hub_generated/module_assets/-2712622/1732054504504/module_-2712622_Site_Search_Input.min.css"> <style> #hs_cos_wrapper_widget_1721162582197 .content-lite { padding:0px; margin-top:0px; margin-bottom:0px; text-align:CENTER; } #hs_cos_wrapper_widget_1721162582197 .content-lite h1, #hs_cos_wrapper_widget_1721162582197 .content-lite h2, #hs_cos_wrapper_widget_1721162582197 .content-lite h3, #hs_cos_wrapper_widget_1721162582197 .content-lite h4, #hs_cos_wrapper_widget_1721162582197 .content-lite h5, #hs_cos_wrapper_widget_1721162582197 .content-lite h6 { color:#ffffff; opacity:1.0; } </style> <style> #hs_cos_wrapper_widget_1721162745442 .form-lite { padding:0px; margin-top:0px; margin-bottom:0px; text-align:LEFT; } </style> <style> @font-face { font-family: "Ubuntu"; font-weight: 400; font-style: normal; font-display: swap; src: url("/_hcms/googlefonts/Ubuntu/regular.woff2") format("woff2"), url("/_hcms/googlefonts/Ubuntu/regular.woff") format("woff"); } @font-face { font-family: "Ubuntu"; font-weight: 700; font-style: normal; font-display: swap; src: url("/_hcms/googlefonts/Ubuntu/700.woff2") format("woff2"), url("/_hcms/googlefonts/Ubuntu/700.woff") format("woff"); } </style> <!-- Editor Styles --> <style id="hs_editor_style" type="text/css"> #hs_cos_wrapper_widget_1695840013043 { display: block !important; padding-bottom: 0px !important; padding-left: 10px !important; padding-right: 10px !important; padding-top: 20px !important } #hs_cos_wrapper_widget_1721161853922 { color: #009e75 !important; display: block !important } #hs_cos_wrapper_widget_1721161853922 p , #hs_cos_wrapper_widget_1721161853922 li , #hs_cos_wrapper_widget_1721161853922 span , #hs_cos_wrapper_widget_1721161853922 label , #hs_cos_wrapper_widget_1721161853922 h1 , #hs_cos_wrapper_widget_1721161853922 h2 , #hs_cos_wrapper_widget_1721161853922 h3 , #hs_cos_wrapper_widget_1721161853922 h4 , #hs_cos_wrapper_widget_1721161853922 h5 , #hs_cos_wrapper_widget_1721161853922 h6 { color: #009e75 !important } #hs_cos_wrapper_widget_1707934728424 { display: block !important; text-align: center !important } #hs_cos_wrapper_widget_1721161853922 { color: #009e75 !important; display: block !important } #hs_cos_wrapper_widget_1721161853922 p , #hs_cos_wrapper_widget_1721161853922 li , #hs_cos_wrapper_widget_1721161853922 span , #hs_cos_wrapper_widget_1721161853922 label , #hs_cos_wrapper_widget_1721161853922 h1 , #hs_cos_wrapper_widget_1721161853922 h2 , #hs_cos_wrapper_widget_1721161853922 h3 , #hs_cos_wrapper_widget_1721161853922 h4 , #hs_cos_wrapper_widget_1721161853922 h5 , #hs_cos_wrapper_widget_1721161853922 h6 { color: #009e75 !important } .widget_1695840013043-flexbox-positioning { display: -ms-flexbox !important; -ms-flex-direction: column !important; -ms-flex-align: start !important; -ms-flex-pack: start; display: flex !important; flex-direction: column !important; align-items: flex-start !important; justify-content: flex-start; } .widget_1695840013043-flexbox-positioning > div { max-width: 100%; flex-shrink: 0 !important; } .widget_1707935050061-flexbox-positioning { display: -ms-flexbox !important; -ms-flex-direction: column !important; -ms-flex-align: center !important; -ms-flex-pack: start; display: flex !important; flex-direction: column !important; align-items: center !important; justify-content: flex-start; } .widget_1707935050061-flexbox-positioning > div { max-width: 100%; flex-shrink: 0 !important; } /* HubSpot Non-stacked Media Query Styles */ @media (min-width:768px) { .header-top-row-0-vertical-alignment > .row-fluid { display: -ms-flexbox !important; -ms-flex-direction: row; display: flex !important; flex-direction: row; } .cell_1695839761992-vertical-alignment { display: -ms-flexbox !important; -ms-flex-direction: column !important; -ms-flex-pack: center !important; display: flex !important; flex-direction: column !important; justify-content: center !important; } .cell_1695839761992-vertical-alignment > div { flex-shrink: 0 !important; } } /* HubSpot Styles (default) */ .header-top-row-0-padding { padding-top: 20px !important; padding-bottom: 10px !important; padding-left: 0px !important; padding-right: 20px !important; } .cell_1695839761992-row-0-padding { padding-top: 0px !important; } .dnd_area-row-0-padding { padding-top: 60px !important; padding-bottom: 60px !important; } .dnd_area-row-0-background-layers { background-image: linear-gradient(rgba(0, 158, 117, 1), rgba(0, 158, 117, 1)) !important; background-position: left top !important; background-size: auto !important; background-repeat: no-repeat !important; } .dnd_area-row-1-padding { padding-top: 35px !important; } .cell_1661522884083-row-1-padding { padding-top: 0px !important; } .footer-row-0-padding { padding-top: 70px !important; padding-bottom: 25px !important; } .cell_17079347285362-row-0-padding { padding-top: 0px !important; } .cell_17079347285362-row-1-padding { padding-top: 13px !important; } </style> <!-- Added by GoogleAnalytics4 integration --> <script> var _hsp = window._hsp = window._hsp || []; window.dataLayer = window.dataLayer || []; function gtag(){dataLayer.push(arguments);} var useGoogleConsentModeV2 = true; var waitForUpdateMillis = 1000; if (!window._hsGoogleConsentRunOnce) { window._hsGoogleConsentRunOnce = true; gtag('consent', 'default', { 'ad_storage': 'denied', 'analytics_storage': 'denied', 'ad_user_data': 'denied', 'ad_personalization': 'denied', 'wait_for_update': waitForUpdateMillis }); if (useGoogleConsentModeV2) { _hsp.push(['useGoogleConsentModeV2']) } else { _hsp.push(['addPrivacyConsentListener', function(consent){ var hasAnalyticsConsent = consent && (consent.allowed || (consent.categories && consent.categories.analytics)); var hasAdsConsent = consent && (consent.allowed || (consent.categories && consent.categories.advertisement)); gtag('consent', 'update', { 'ad_storage': hasAdsConsent ? 'granted' : 'denied', 'analytics_storage': hasAnalyticsConsent ? 'granted' : 'denied', 'ad_user_data': hasAdsConsent ? 'granted' : 'denied', 'ad_personalization': hasAdsConsent ? 'granted' : 'denied' }); }]); } } gtag('js', new Date()); gtag('set', 'developer_id.dZTQ1Zm', true); gtag('config', 'G-X6ZGZ4VPT4'); </script> <script async src="https://www.googletagmanager.com/gtag/js?id=G-X6ZGZ4VPT4"></script> <!-- /Added by GoogleAnalytics4 integration --> <!-- Added by GoogleTagManager integration --> <script> var _hsp = window._hsp = window._hsp || []; window.dataLayer = window.dataLayer || []; function gtag(){dataLayer.push(arguments);} var useGoogleConsentModeV2 = true; var waitForUpdateMillis = 1000; var hsLoadGtm = function loadGtm() { if(window._hsGtmLoadOnce) { return; } if (useGoogleConsentModeV2) { gtag('set','developer_id.dZTQ1Zm',true); gtag('consent', 'default', { 'ad_storage': 'denied', 'analytics_storage': 'denied', 'ad_user_data': 'denied', 'ad_personalization': 'denied', 'wait_for_update': waitForUpdateMillis }); _hsp.push(['useGoogleConsentModeV2']) } (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start': new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0], j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src= 'https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f); })(window,document,'script','dataLayer','GTM-KF3TM6'); window._hsGtmLoadOnce = true; }; _hsp.push(['addPrivacyConsentListener', function(consent){ if(consent.allowed || (consent.categories && consent.categories.analytics)){ hsLoadGtm(); } }]); </script> <!-- /Added by GoogleTagManager integration --> <link rel="canonical" href="https://web.genewiz.com/tech-note/pbmc-isolation-cryopreservation"> <!-- Google tag (gtag.js) --> <script async src="https://www.googletagmanager.com/gtag/js?id=AW-1072435208"></script> <script> window.dataLayer = window.dataLayer || []; function gtag(){dataLayer.push(arguments);} gtag('js', new Date()); gtag('config', 'AW-1072435208'); </script> <!-- Google Tag Manager --> <script>(function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start': new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0], j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src= 'https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f); })(window,document,'script','dataLayer','GTM-KF3TM6');</script> <!-- End Google Tag Manager --> <script src="https://use.typekit.net/gll5nnj.js"></script> <script>try{Typekit.load({ async: true });}catch(e){}</script> <!-- FontAwesome --> <script src="https://use.fontawesome.com/75c29f9a51.js"></script> <!-- Facebook Pixel Code --> <script> !function(f,b,e,v,n,t,s){if(f.fbq)return;n=f.fbq=function(){n.callMethod? n.callMethod.apply(n,arguments):n.queue.push(arguments)};if(!f._fbq)f._fbq=n; n.push=n;n.loaded=!0;n.version='2.0';n.queue=[];t=b.createElement(e);t.async=!0; t.src=v;s=b.getElementsByTagName(e)[0];s.parentNode.insertBefore(t,s)}(window, document,'script','https://connect.facebook.net/en_US/fbevents.js'); fbq('init', '348185225613720'); // Insert your pixel ID here. fbq('track', 'PageView'); </script> <noscript>&lt;img height="1" width="1" style="display:none" src="https://www.facebook.com/tr?id=348185225613720&amp;amp;ev=PageView&amp;amp;noscript=1"&gt;</noscript> <!-- DO NOT MODIFY --> <!-- End Facebook Pixel Code --> <!-- Google Code for Clicked Order Now Button Conversion Page In your html page, add the snippet and call goog_report_conversion when someone clicks on the chosen link or button. --> <script type="text/javascript"> /* <![CDATA[ */ goog_snippet_vars = function() { var w = window; w.google_conversion_id = 1072435208; w.google_conversion_label = "iXPjCLLEznUQiKCw_wM"; w.google_remarketing_only = false; } // DO NOT CHANGE THE CODE BELOW. goog_report_conversion = function(url) { goog_snippet_vars(); window.google_conversion_format = "3"; var opt = new Object(); opt.onload_callback = function() { if (typeof(url) != 'undefined') { window.location = url; } } var conv_handler = window['google_trackConversion']; if (typeof(conv_handler) == 'function') { conv_handler(opt); } } /* ]]> */ </script> <script type="text/javascript" src="//www.googleadservices.com/pagead/conversion_async.js"> </script> <!-- Auryc --> <!--<script data-cfasync="false" charset="utf-8" > ;(function (g) { g.aurycReadyCb = g.aurycReadyCb || []; var d = document, i, am = d.createElement("script"), h = d.head || d.getElementsByTagName("head")[0],aex = { "src": "//cdn.userty.com/202-genewizcom/container.js", "data-cfasync": "false", "async": "true", "defer": "true", "data-vendor": "userty", "data-role": "container", "charset": "utf-8" }; for (var attr in aex) { am.setAttribute(attr,aex[attr]); } h.appendChild(am); })(window);--> <!--</script>--> <!-- Hotjar Tracking Code for https://web.genewiz.com --> <script> (function(h,o,t,j,a,r){ h.hj=h.hj||function(){(h.hj.q=h.hj.q||[]).push(arguments)}; h._hjSettings={hjid:1063981,hjsv:6}; a=o.getElementsByTagName('head')[0]; r=o.createElement('script');r.async=1; r.src=t+h._hjSettings.hjid+j+h._hjSettings.hjsv; a.appendChild(r); })(window,document,'https://static.hotjar.com/c/hotjar-','.js?sv='); </script> <meta property="og:image" content="https://web.genewiz.com/hubfs/2022-01%20PCS%20-%20PBMC%20Isolation%20Tech%20Note/PBMC%20Tech%20Note%20Cover%20Photo-1.png"> <meta property="og:image:width" content="1220"> <meta property="og:image:height" content="1380"> <meta property="og:image:alt" content="azenta cryogenics tech note"> <meta name="twitter:image" content="https://web.genewiz.com/hubfs/2022-01%20PCS%20-%20PBMC%20Isolation%20Tech%20Note/PBMC%20Tech%20Note%20Cover%20Photo-1.png"> <meta name="twitter:image:alt" content="azenta cryogenics tech note"> <meta property="og:url" content="https://web.genewiz.com/tech-note/pbmc-isolation-cryopreservation"> <meta name="twitter:card" content="summary_large_image"> <meta http-equiv="content-language" content="en"> <meta name="generator" content="HubSpot"></head> <body> <!-- Added by GoogleTagManager integration --> <noscript><iframe src="https://www.googletagmanager.com/ns.html?id=GTM-KF3TM6" height="0" width="0" style="display:none;visibility:hidden"></iframe></noscript> <!-- /Added by GoogleTagManager integration --> <div class="body-wrapper hs-content-id-52056861452 hs-landing-page hs-page "> <div data-global-resource-path="Azenta Templates/Form Conversion LP Template/templates/partials/header.html"><header class="header"> <a href="#main-content" class="header_skip">Skip to content</a> <div class="container-fluid"> <div class="row-fluid-wrapper"> <div class="row-fluid"> <div class="span12 widget-span widget-type-cell " style="" data-widget-type="cell" data-x="0" data-w="12"> <div class="row-fluid-wrapper row-depth-1 row-number-1 dnd-section header-top-row-0-padding header-top-row-0-vertical-alignment"> <div class="row-fluid "> <div class="span12 widget-span widget-type-cell dnd-column cell_1695839761992-vertical-alignment" style="" data-widget-type="cell" data-x="0" data-w="12"> <div class="row-fluid-wrapper row-depth-1 row-number-2 cell_1695839761992-row-0-padding dnd-row"> <div class="row-fluid "> <div class="span12 widget-span widget-type-custom_widget widget_1695840013043-flexbox-positioning dnd-module" style="" data-widget-type="custom_widget" data-x="0" data-w="12"> <div id="hs_cos_wrapper_widget_1695840013043" class="hs_cos_wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_module widget-type-logo" style="" data-hs-cos-general-type="widget" data-hs-cos-type="module"> <span id="hs_cos_wrapper_widget_1695840013043_hs_logo_widget" class="hs_cos_wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_logo" style="" data-hs-cos-general-type="widget" data-hs-cos-type="logo"><a href="https://www.genewiz.com/" target="_blank" id="hs-link-widget_1695840013043_hs_logo_widget" rel="noopener" style="border-width:0px;border:0px;"><img src="https://web.genewiz.com/hs-fs/hubfs/GENEWIZ%20Brand%20Assets/GENEWIZ%20Logos/2024-03%20Current%20GENEWIZ%20Logos/GENEWIZ_Logo_Hubspot_250x83@3x.png?width=250&amp;height=83&amp;name=GENEWIZ_Logo_Hubspot_250x83@3x.png" class="hs-image-widget " height="83" style="height: auto;width:250px;border-width:0px;border:0px;" width="250" alt="GENEWIZ from Azenta Life Sciences" title="GENEWIZ from Azenta Life Sciences" srcset="https://web.genewiz.com/hs-fs/hubfs/GENEWIZ%20Brand%20Assets/GENEWIZ%20Logos/2024-03%20Current%20GENEWIZ%20Logos/GENEWIZ_Logo_Hubspot_250x83@3x.png?width=125&amp;height=42&amp;name=GENEWIZ_Logo_Hubspot_250x83@3x.png 125w, https://web.genewiz.com/hs-fs/hubfs/GENEWIZ%20Brand%20Assets/GENEWIZ%20Logos/2024-03%20Current%20GENEWIZ%20Logos/GENEWIZ_Logo_Hubspot_250x83@3x.png?width=250&amp;height=83&amp;name=GENEWIZ_Logo_Hubspot_250x83@3x.png 250w, https://web.genewiz.com/hs-fs/hubfs/GENEWIZ%20Brand%20Assets/GENEWIZ%20Logos/2024-03%20Current%20GENEWIZ%20Logos/GENEWIZ_Logo_Hubspot_250x83@3x.png?width=375&amp;height=125&amp;name=GENEWIZ_Logo_Hubspot_250x83@3x.png 375w, https://web.genewiz.com/hs-fs/hubfs/GENEWIZ%20Brand%20Assets/GENEWIZ%20Logos/2024-03%20Current%20GENEWIZ%20Logos/GENEWIZ_Logo_Hubspot_250x83@3x.png?width=500&amp;height=166&amp;name=GENEWIZ_Logo_Hubspot_250x83@3x.png 500w, https://web.genewiz.com/hs-fs/hubfs/GENEWIZ%20Brand%20Assets/GENEWIZ%20Logos/2024-03%20Current%20GENEWIZ%20Logos/GENEWIZ_Logo_Hubspot_250x83@3x.png?width=625&amp;height=208&amp;name=GENEWIZ_Logo_Hubspot_250x83@3x.png 625w, https://web.genewiz.com/hs-fs/hubfs/GENEWIZ%20Brand%20Assets/GENEWIZ%20Logos/2024-03%20Current%20GENEWIZ%20Logos/GENEWIZ_Logo_Hubspot_250x83@3x.png?width=750&amp;height=249&amp;name=GENEWIZ_Logo_Hubspot_250x83@3x.png 750w" sizes="(max-width: 250px) 100vw, 250px"></a></span> </div> </div><!--end widget-span --> </div><!--end row--> </div><!--end row-wrapper --> </div><!--end widget-span --> </div><!--end row--> </div><!--end row-wrapper --> </div><!--end widget-span --> </div> </div> </div> <div class="hidden"> <div class="header-lite-language-switcher"> <div class="header-language-switcher-label"> <div id="hs_cos_wrapper_language-switcher" class="hs_cos_wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_module widget-type-language_switcher" style="" data-hs-cos-general-type="widget" data-hs-cos-type="module"><span id="hs_cos_wrapper_language-switcher_" class="hs_cos_wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_language_switcher" style="" data-hs-cos-general-type="widget" data-hs-cos-type="language_switcher"></span></div> <div class="header-language-switcher-label-current"> English</div> </div> </div> </div> <div class="header-lite-search-trigger flex items-center hidden"> <svg version="1.0" xmlns="http://www.w3.org/2000/svg" viewbox="0 0 512 512" aria-hidden="true"><g id="search1_layer"><path d="M505 442.7L405.3 343c-4.5-4.5-10.6-7-17-7H372c27.6-35.3 44-79.7 44-128C416 93.1 322.9 0 208 0S0 93.1 0 208s93.1 208 208 208c48.3 0 92.7-16.4 128-44v16.3c0 6.4 2.5 12.5 7 17l99.7 99.7c9.4 9.4 24.6 9.4 33.9 0l28.3-28.3c9.4-9.4 9.4-24.6.1-34zM208 336c-70.7 0-128-57.2-128-128 0-70.7 57.2-128 128-128 70.7 0 128 57.2 128 128 0 70.7-57.2 128-128 128z" /></g></svg> </div> <div class="header-lite-search"> <div class="relative"> <div class="header-lite-search_top block absolute"> <div class="header-lite-search-close"> <div class="hamburger hamburger--slider js-hamburger is-active flex items-center justify-center h-full"> <div class="hamburger-box"> <div class="hamburger-inner"></div> </div> </div> </div> </div> <div id="hs_cos_wrapper_site_search" class="hs_cos_wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_module" style="" data-hs-cos-general-type="widget" data-hs-cos-type="module"> <div class="hs-search-field"> <div class="hs-search-field__bar"> <form data-hs-do-not-collect="true" action="/hs-search-results"> <input type="text" class="hs-search-field__input" name="term" autocomplete="off" aria-label="Search" placeholder="Search"> <input type="hidden" name="type" value="SITE_PAGE"> <input type="hidden" name="type" value="BLOG_POST"> <input type="hidden" name="type" value="LISTING_PAGE"> </form> </div> <ul class="hs-search-field__suggestions"></ul> </div></div> </div> </div> </header></div> <div id="main-content"> <main id="main-content" class="body-container-wrapper"> <div class="container-fluid body-container body-container--home"> <div class="row-fluid-wrapper"> <div class="row-fluid"> <div class="span12 widget-span widget-type-cell dnd-area" style="" data-widget-type="cell" data-x="0" data-w="12"> <div class="row-fluid-wrapper row-depth-1 row-number-1 dnd_area-row-0-background-color dnd_area-row-0-background-layers dnd-section dnd_area-row-0-padding"> <div class="row-fluid "> <div class="span12 widget-span widget-type-cell dnd-column" style="" data-widget-type="cell" data-x="0" data-w="12"> <div class="row-fluid-wrapper row-depth-1 row-number-2 dnd-row"> <div class="row-fluid "> <div class="span12 widget-span widget-type-custom_widget dnd-module" style="" data-widget-type="custom_widget" data-x="0" data-w="12"> <div id="hs_cos_wrapper_widget_1721162582197" class="hs_cos_wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_module" style="" data-hs-cos-general-type="widget" data-hs-cos-type="module"><div class="content-lite relative "> <h1>An Optimized Approach to PBMC Isolation and Cryopreservation</h1> </div> </div> </div><!--end widget-span --> </div><!--end row--> </div><!--end row-wrapper --> </div><!--end widget-span --> </div><!--end row--> </div><!--end row-wrapper --> <div class="row-fluid-wrapper row-depth-1 row-number-3 dnd_area-row-1-padding dnd-section"> <div class="row-fluid "> <div class="span6 widget-span widget-type-cell dnd-column" style="" data-widget-type="cell" data-x="0" data-w="6"> <div class="row-fluid-wrapper row-depth-1 row-number-4 dnd-row"> <div class="row-fluid "> <div class="span12 widget-span widget-type-custom_widget dnd-module" style="" data-widget-type="custom_widget" data-x="0" data-w="12"> <div id="hs_cos_wrapper_widget_1721161853922" class="hs_cos_wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_module widget-type-text" style="" data-hs-cos-general-type="widget" data-hs-cos-type="module"><span id="hs_cos_wrapper_widget_1721161853922_" class="hs_cos_wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_text" style="" data-hs-cos-general-type="widget" data-hs-cos-type="text">TECH NOTE</span></div> </div><!--end widget-span --> </div><!--end row--> </div><!--end row-wrapper --> <div class="row-fluid-wrapper row-depth-1 row-number-5 cell_1661522884083-row-1-padding dnd-row"> <div class="row-fluid "> <div class="span12 widget-span widget-type-custom_widget dnd-module" style="" data-widget-type="custom_widget" data-x="0" data-w="12"> <div id="hs_cos_wrapper_dnd-modulemodule_164459845601510218" class="hs_cos_wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_module widget-type-rich_text" style="" data-hs-cos-general-type="widget" data-hs-cos-type="module"><span id="hs_cos_wrapper_dnd-modulemodule_164459845601510218_" class="hs_cos_wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_rich_text" style="" data-hs-cos-general-type="widget" data-hs-cos-type="rich_text"><h2>An Optimized Approach to PBMC Isolation and Cryopreservation</h2> <p style="font-size: 16px; line-height: 1.5;"><span style="color: #3a3a3a;">Peripheral blood mononuclear cells (PBMCs) are a valuable sample type for basic research and clinical studies. The quantity and quality of these isolated cells are critical to the performance of functional assays, as poor sample handling may result in inaccurate data. In this tech note, we discuss the challenges associated with PBMC isolation and offer a robust workflow for PBMC isolation and cryopreservation, ensuring high-quality results that exceed industry standards for cell viability and yield.</span></p> <h3>What You'll Learn</h3> <ul> <li>The importance of quantity and quality of isolated PBMC cells for the downstream analysis</li> <li>The challenges in preanalytical steps of PBMC isolation that affect sample quality</li> <li>How Azenta Life Sciences optimized workflows for the rapid isolation and freezing of PBMCs with reproducible, best-in-class results</li> </ul></span></div> </div><!--end widget-span --> </div><!--end row--> </div><!--end row-wrapper --> <div class="row-fluid-wrapper row-depth-1 row-number-6 dnd-row"> <div class="row-fluid "> <div class="span12 widget-span widget-type-custom_widget dnd-module" style="" data-widget-type="custom_widget" data-x="0" data-w="12"> <div id="hs_cos_wrapper_dnd-modulemodule_15785536722931530" class="hs_cos_wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_module widget-type-linked_image widget-type-linked_image widget-type-linked_image" style="" data-hs-cos-general-type="widget" data-hs-cos-type="module"> <span id="hs_cos_wrapper_dnd-modulemodule_15785536722931530_" class="hs_cos_wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_linked_image" style="" data-hs-cos-general-type="widget" data-hs-cos-type="linked_image"><img src="https://web.genewiz.com/hubfs/2022-01%20PCS%20-%20PBMC%20Isolation%20Tech%20Note/PBMC%20Tech%20Note%20Cover%20Photo-1.png" class="hs-image-widget " style="max-width: 100%; height: auto;" alt="PBMC Tech Note Cover Photo-1" title="PBMC Tech Note Cover Photo-1"></span></div> </div><!--end widget-span --> </div><!--end row--> </div><!--end row-wrapper --> </div><!--end widget-span --> <div class="span6 widget-span widget-type-cell dnd-column" style="" data-widget-type="cell" data-x="6" data-w="6"> <div class="row-fluid-wrapper row-depth-1 row-number-7 dnd-row"> <div class="row-fluid "> <div class="span12 widget-span widget-type-custom_widget dnd-module" style="" data-widget-type="custom_widget" data-x="0" data-w="12"> <div id="hs_cos_wrapper_widget_1721162745442" class="hs_cos_wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_module" style="" data-hs-cos-general-type="widget" data-hs-cos-type="module"><div class="form-lite relative "> <div class="mb-8"><h2>Download Tech Note</h2></div> <div class=""> <span id="hs_cos_wrapper_widget_1721162745442_" class="hs_cos_wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_form" style="" data-hs-cos-general-type="widget" data-hs-cos-type="form"><h3 id="hs_cos_wrapper_form_683415801_title" class="hs_cos_wrapper form-title" data-hs-cos-general-type="widget_field" data-hs-cos-type="text"></h3> <div id="hs_form_target_form_683415801"></div> </span> </div> </div> </div> </div><!--end widget-span --> </div><!--end row--> </div><!--end row-wrapper --> </div><!--end widget-span --> </div><!--end row--> </div><!--end row-wrapper --> </div><!--end widget-span --> </div> </div> </div> </main> </div> <div data-global-resource-path="Azenta Templates/Form Conversion LP Template/templates/partials/footer.html"><footer class="footer test"> <div class="container-fluid footer__container content-wrapper"> <div class="row-fluid-wrapper"> <div class="row-fluid"> <div class="span12 widget-span widget-type-cell " style="" data-widget-type="cell" data-x="0" data-w="12"> <div class="row-fluid-wrapper row-depth-1 row-number-1 dnd-section footer-row-0-padding"> <div class="row-fluid "> <div class="span12 widget-span widget-type-cell dnd-column" style="" data-widget-type="cell" data-x="0" data-w="12"> <div class="row-fluid-wrapper row-depth-1 row-number-2 cell_17079347285362-row-0-padding dnd-row"> <div class="row-fluid "> <div class="span12 widget-span widget-type-custom_widget widget_1707935050061-flexbox-positioning dnd-module" style="" data-widget-type="custom_widget" data-x="0" data-w="12"> <div id="hs_cos_wrapper_widget_1707935050061" class="hs_cos_wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_module widget-type-logo" style="" data-hs-cos-general-type="widget" data-hs-cos-type="module"> <span id="hs_cos_wrapper_widget_1707935050061_hs_logo_widget" class="hs_cos_wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_logo" style="" data-hs-cos-general-type="widget" data-hs-cos-type="logo"><a href="https://www.azenta.com/" id="hs-link-widget_1707935050061_hs_logo_widget" style="border-width:0px;border:0px;"><img src="https://web.genewiz.com/hs-fs/hubfs/ARCHIVE/Azenta%20Logo%20and%20Brand%20Assets/Azenta%20Logos/Azenta_Logo_Primary_FullColor-Black_300x100@2x.png?width=250&amp;height=83&amp;name=Azenta_Logo_Primary_FullColor-Black_300x100@2x.png" class="hs-image-widget " height="83" style="height: auto;width:250px;border-width:0px;border:0px;" width="250" alt="Azenta_Logo_Primary_FullColor-Black_300x100@2x" title="Azenta_Logo_Primary_FullColor-Black_300x100@2x" srcset="https://web.genewiz.com/hs-fs/hubfs/ARCHIVE/Azenta%20Logo%20and%20Brand%20Assets/Azenta%20Logos/Azenta_Logo_Primary_FullColor-Black_300x100@2x.png?width=125&amp;height=42&amp;name=Azenta_Logo_Primary_FullColor-Black_300x100@2x.png 125w, https://web.genewiz.com/hs-fs/hubfs/ARCHIVE/Azenta%20Logo%20and%20Brand%20Assets/Azenta%20Logos/Azenta_Logo_Primary_FullColor-Black_300x100@2x.png?width=250&amp;height=83&amp;name=Azenta_Logo_Primary_FullColor-Black_300x100@2x.png 250w, https://web.genewiz.com/hs-fs/hubfs/ARCHIVE/Azenta%20Logo%20and%20Brand%20Assets/Azenta%20Logos/Azenta_Logo_Primary_FullColor-Black_300x100@2x.png?width=375&amp;height=125&amp;name=Azenta_Logo_Primary_FullColor-Black_300x100@2x.png 375w, https://web.genewiz.com/hs-fs/hubfs/ARCHIVE/Azenta%20Logo%20and%20Brand%20Assets/Azenta%20Logos/Azenta_Logo_Primary_FullColor-Black_300x100@2x.png?width=500&amp;height=166&amp;name=Azenta_Logo_Primary_FullColor-Black_300x100@2x.png 500w, https://web.genewiz.com/hs-fs/hubfs/ARCHIVE/Azenta%20Logo%20and%20Brand%20Assets/Azenta%20Logos/Azenta_Logo_Primary_FullColor-Black_300x100@2x.png?width=625&amp;height=208&amp;name=Azenta_Logo_Primary_FullColor-Black_300x100@2x.png 625w, https://web.genewiz.com/hs-fs/hubfs/ARCHIVE/Azenta%20Logo%20and%20Brand%20Assets/Azenta%20Logos/Azenta_Logo_Primary_FullColor-Black_300x100@2x.png?width=750&amp;height=249&amp;name=Azenta_Logo_Primary_FullColor-Black_300x100@2x.png 750w" sizes="(max-width: 250px) 100vw, 250px"></a></span> </div> </div><!--end widget-span --> </div><!--end row--> </div><!--end row-wrapper --> <div class="row-fluid-wrapper row-depth-1 row-number-3 cell_17079347285362-row-1-padding dnd-row"> <div class="row-fluid "> <div class="span12 widget-span widget-type-custom_widget dnd-module" style="" data-widget-type="custom_widget" data-x="0" data-w="12"> <div id="hs_cos_wrapper_widget_1707934728424" class="hs_cos_wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_module widget-type-page_footer" style="" data-hs-cos-general-type="widget" data-hs-cos-type="module"><span id="hs_cos_wrapper_widget_1707934728424_page_footer" class="hs_cos_wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_page_footer" style="" data-hs-cos-general-type="widget" data-hs-cos-type="page_footer"> <footer> <span class="hs-footer-company-copyright">© 2024 Azenta Life Sciences</span> </footer> </span></div> </div><!--end widget-span --> </div><!--end row--> </div><!--end row-wrapper --> </div><!--end widget-span --> </div><!--end row--> </div><!--end row-wrapper --> </div><!--end widget-span --> </div> </div> </div> </footer></div> </div> <!-- HubSpot performance collection script --> <script defer src="/hs/hsstatic/content-cwv-embed/static-1.1293/embed.js"></script> <script defer src="https://web.genewiz.com/hs-fs/hub/3478602/hub_generated/template_assets/69476802372/1721068289194/Azenta_Templates/Form_Conversion_LP_Template/js/plugins/plugins.min.js"></script> <script defer src="https://web.genewiz.com/hs-fs/hub/3478602/hub_generated/template_assets/69476645745/1721068285014/Azenta_Templates/Form_Conversion_LP_Template/js/main.min.js"></script> <script> var hsVars = hsVars || {}; hsVars['language'] = 'en'; </script> <script src="/hs/hsstatic/cos-i18n/static-1.53/bundles/project.js"></script> <script src="https://cdn2.hubspot.net/hub/-1/hub_generated/module_assets/-2712622/1732054503738/module_-2712622_Site_Search_Input.min.js"></script> <!--[if lte IE 8]> <script charset="utf-8" src="https://js.hsforms.net/forms/v2-legacy.js"></script> <![endif]--> <script data-hs-allowed="true" src="/_hcms/forms/v2.js"></script> <script data-hs-allowed="true"> var options = { portalId: '3478602', formId: 'a34a7f48-ecda-4d0c-9f3d-4e517465e994', formInstanceId: '4344', pageId: '52056861452', region: 'na1', pageName: "PBMC Isolation and Cryopreservation Tech Note | GENEWIZ from Azenta", css: '', target: '#hs_form_target_form_683415801', contentType: "landing-page", formsBaseUrl: '/_hcms/forms/', formData: { cssClass: 'hs-form stacked hs-custom-form' } }; options.getExtraMetaDataBeforeSubmit = function() { var metadata = {}; if (hbspt.targetedContentMetadata) { var count = hbspt.targetedContentMetadata.length; var targetedContentData = []; for (var i = 0; i < count; i++) { var tc = hbspt.targetedContentMetadata[i]; if ( tc.length !== 3) { continue; } targetedContentData.push({ definitionId: tc[0], criterionId: tc[1], smartTypeId: tc[2] }); } metadata["targetedContentMetadata"] = JSON.stringify(targetedContentData); } return metadata; }; hbspt.forms.create(options); </script> <!-- Start of HubSpot Analytics Code --> <script type="text/javascript"> var _hsq = _hsq || []; _hsq.push(["setContentType", "landing-page"]); _hsq.push(["setCanonicalUrl", "https:\/\/web.genewiz.com\/tech-note\/pbmc-isolation-cryopreservation"]); _hsq.push(["setPageId", "52056861452"]); _hsq.push(["setContentMetadata", { "contentPageId": 52056861452, "legacyPageId": "52056861452", "contentFolderId": null, "contentGroupId": null, "abTestId": null, "languageVariantId": 52056861452, "languageCode": "en", }]); </script> <script type="text/javascript" id="hs-script-loader" async defer src="/hs/scriptloader/3478602.js?businessUnitId=0"></script> <!-- End of HubSpot Analytics Code --> <script type="text/javascript"> var hsVars = { render_id: "3a1986eb-ae37-4a8c-aaa5-e8bf9343f575", ticks: 1732069420567, page_id: 52056861452, content_group_id: 0, portal_id: 3478602, app_hs_base_url: "https://app.hubspot.com", cp_hs_base_url: "https://cp.hubspot.com", language: "en", analytics_page_type: "landing-page", scp_content_type: "", analytics_page_id: "52056861452", category_id: 1, folder_id: 0, is_hubspot_user: false } </script> <script defer src="/hs/hsstatic/HubspotToolsMenu/static-1.354/js/index.js"></script> <!-- Google Code for Remarketing Tag --> <script type="text/javascript"> /* <![CDATA[ */ var google_conversion_id = 1072435208; var google_custom_params = window.google_tag_params; var google_remarketing_only = true; /* ]]> */ </script> <script type="text/javascript" src="//www.googleadservices.com/pagead/conversion.js"></script> <noscript> <div style="display:inline;"> <img height="1" width="1" style="border-style:none;" alt="" src="//googleads.g.doubleclick.net/pagead/viewthroughconversion/1072435208/?guid=ON&amp;script=0"> </div> </noscript> </body></html>

Pages: 1 2 3 4 5 6 7 8 9 10