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spectrometry</title> <meta name="description" content="Search results for: mass spectrometry"> <meta name="keywords" content="mass spectrometry"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none 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action="https://publications.waset.org/abstracts/search"> <div id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="mass spectrometry"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 3675</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: mass spectrometry</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3675</span> A Plasmonic Mass Spectrometry Approach for Detection of Small Nutrients and Toxins</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Haiyang%20Su">Haiyang Su</a>, <a href="https://publications.waset.org/abstracts/search?q=Kun%20Qian"> Kun Qian</a> </p> <p class="card-text"><strong>Abstract:</strong></p> We developed a novel plasmonic matrix assisted laser desorption/ionization mass spectrometry (MALDI MS) approach to detect small nutrients and toxin in complex biological emulsion samples. We used silver nanoshells (SiO₂@Ag) with optimized structures as matrices and achieved direct analysis of ~6 nL of human breast milk without any enrichment or separation. We performed identification and quantitation of small nutrients and toxins with limit-of-detection down to 0.4 pmol (for melamine) and reaction time shortened to minutes, superior to the conventional biochemical methods currently in use. Our approach contributed to the near-future application of MALDI MS in a broad field and personalized design of plasmonic materials for real case bio-analysis. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=plasmonic%20materials" title="plasmonic materials">plasmonic materials</a>, <a href="https://publications.waset.org/abstracts/search?q=laser%20desorption%2Fionization" title=" laser desorption/ionization"> laser desorption/ionization</a>, <a href="https://publications.waset.org/abstracts/search?q=mass%20spectrometry" title=" mass spectrometry"> mass spectrometry</a>, <a href="https://publications.waset.org/abstracts/search?q=small%20nutrients" title=" small nutrients"> small nutrients</a>, <a href="https://publications.waset.org/abstracts/search?q=toxins" title=" toxins"> toxins</a> </p> <a href="https://publications.waset.org/abstracts/90310/a-plasmonic-mass-spectrometry-approach-for-detection-of-small-nutrients-and-toxins" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/90310.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">211</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3674</span> New Method for the Determination of Montelukast in Human Plasma by Solid Phase Extraction Using Liquid Chromatography Tandem Mass Spectrometry</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Vijayalakshmi%20Marella">Vijayalakshmi Marella</a>, <a href="https://publications.waset.org/abstracts/search?q=NageswaraRaoPilli"> NageswaraRaoPilli</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This paper describes a simple, rapid and sensitive liquid chromatography / tandem mass spectrometry assay for the determination of montelukast in human plasma using montelukast d6 as an internal standard. Analyte and the internal standard were extracted from 50 µL of human plasma via solid phase extraction technique without evaporation, drying and reconstitution steps. The chromatographic separation was achieved on a C18 column by using a mixture of methanol and 5mM ammonium acetate (80:20, v/v) as the mobile phase at a flow rate of 0.8 mL/min. Good linearity results were obtained during the entire course of validation. Method validation was performed as per FDA guidelines and the results met the acceptance criteria. A run time of 2.5 min for each sample made it possible to analyze more number of samples in short time, thus increasing the productivity. The proposed method was found to be applicable to clinical studies. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Montelukast" title="Montelukast">Montelukast</a>, <a href="https://publications.waset.org/abstracts/search?q=tandem%20mass%20spectrometry" title=" tandem mass spectrometry"> tandem mass spectrometry</a>, <a href="https://publications.waset.org/abstracts/search?q=montelukast%20d6" title=" montelukast d6"> montelukast d6</a>, <a href="https://publications.waset.org/abstracts/search?q=FDA%20guidelines" title=" FDA guidelines"> FDA guidelines</a> </p> <a href="https://publications.waset.org/abstracts/29304/new-method-for-the-determination-of-montelukast-in-human-plasma-by-solid-phase-extraction-using-liquid-chromatography-tandem-mass-spectrometry" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/29304.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">315</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3673</span> Fuel Oxidation Reactions: Pathways and Reactive Intermediates Characterization via Synchrotron Photoionization Mass Spectrometry</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Giovanni%20Meloni">Giovanni Meloni</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Recent results are presented from experiments carried out at the Advanced Light Source (ALS) at the Chemical Dynamics Beamline of Lawrence Berkeley National Laboratory using multiplexed synchrotron photoionization mass spectrometry. The reaction mixture and a buffer gas (He) are introduced through individually calibrated mass flow controllers into a quartz slow flow reactor held at constant pressure and temperature. The gaseous mixture effuses through a 650 μm pinhole into a 1.5 mm skimmer, forming a molecular beam that enters a differentially pumped ionizing chamber. The molecular beam is orthogonally intersected by a tunable synchrotron radiation produced by the ALS in the 8-11 eV energy range. Resultant ions are accelerated, collimated, and focused into an orthogonal time-of-flight mass spectrometer. Reaction species are identified by their mass-to-charge ratios and photoionization (PI) spectra. Comparison of experimental PI spectra with literature and/or simulated curves is routinely done to assure the identity of a given species. With the aid of electronic structure calculations, potential energy surface scans are performed, and Franck-Condon spectral simulations are obtained. Examples of these experiments are discussed, ranging from new intermediates characterization to reaction mechanisms elucidation and biofuels oxidation pathways identification. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=mass%20spectrometry" title="mass spectrometry">mass spectrometry</a>, <a href="https://publications.waset.org/abstracts/search?q=reaction%20intermediates" title=" reaction intermediates"> reaction intermediates</a>, <a href="https://publications.waset.org/abstracts/search?q=synchrotron%20photoionization" title=" synchrotron photoionization"> synchrotron photoionization</a>, <a href="https://publications.waset.org/abstracts/search?q=oxidation%20reactions" title=" oxidation reactions"> oxidation reactions</a> </p> <a href="https://publications.waset.org/abstracts/173937/fuel-oxidation-reactions-pathways-and-reactive-intermediates-characterization-via-synchrotron-photoionization-mass-spectrometry" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/173937.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">73</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3672</span> A Step-by-Step Analytical Protocol For Detecting and Identifying Minor Differences In Like Materials and Polymers Using Pyrolysis -Gas Chromatography/Mass Spectrometry Technique</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Athena%20Nguyen">Athena Nguyen</a>, <a href="https://publications.waset.org/abstracts/search?q=Rojin%20Belganeh"> Rojin Belganeh</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Detecting and identifying differences in like polymer materials are key factors in failure and deformulation analysis, and reverse engineering. Pyrolysis-GC/MS is an easy solid sample introduction technique which expands the application areas of gas chromatography and mass spectrometry. The Micro furnace pyrolyzer is directly interfaced with the GC injector preventing any potential of cold spot, carryover, and cross contamination. In this presentation, the analysis of the differences in three polystyrene samples is demonstrated. Although the three samples look very similar by Evolve gas analysis (EGA) and Flash pyrolysis, there are indications of small levels of other materials. By performing Thermal desorption-GC/MS, the additive compounds between samples show the differences. EGA, flash pyrolysis, and thermal desorption analysis are the different modes of operations of the micro-furnace pyrolyzer enabling users to perform multiple analytical techniques. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Gas%20chromatography%2FMass%20spectrometry" title="Gas chromatography/Mass spectrometry">Gas chromatography/Mass spectrometry</a>, <a href="https://publications.waset.org/abstracts/search?q=pyrolysis" title=" pyrolysis"> pyrolysis</a>, <a href="https://publications.waset.org/abstracts/search?q=pyrolyzer" title=" pyrolyzer"> pyrolyzer</a>, <a href="https://publications.waset.org/abstracts/search?q=thermal%20desorption-GC%2FMS" title=" thermal desorption-GC/MS"> thermal desorption-GC/MS</a> </p> <a href="https://publications.waset.org/abstracts/139716/a-step-by-step-analytical-protocol-for-detecting-and-identifying-minor-differences-in-like-materials-and-polymers-using-pyrolysis-gas-chromatographymass-spectrometry-technique" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/139716.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">187</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3671</span> De-Novo Structural Elucidation from Mass/NMR Spectra</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ismael%20Zamora">Ismael Zamora</a>, <a href="https://publications.waset.org/abstracts/search?q=Elisabeth%20Ortega"> Elisabeth Ortega</a>, <a href="https://publications.waset.org/abstracts/search?q=Tatiana%20Radchenko"> Tatiana Radchenko</a>, <a href="https://publications.waset.org/abstracts/search?q=Guillem%20Plasencia"> Guillem Plasencia</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The structure elucidation based on Mass Spectra (MS) data of unknown substances is an unresolved problem that affects many different fields of application. The recent overview of software available for structure elucidation of small molecules has shown the demand for efficient computational tool that will be able to perform structure elucidation of unknown small molecules and peptides. We developed an algorithm for De-Novo fragment analysis based on MS data that proposes a set of scored and ranked structures that are compatible with the MS and MSMS spectra. Several different algorithms were developed depending on the initial set of fragments and the structure building processes. Also, in all cases, several scores for the final molecule ranking were computed. They were validated with small and middle databases (DB) with the eleven test set compounds. Similar results were obtained from any of the databases that contained the fragments of the expected compound. We presented an algorithm. Or De-Novo fragment analysis based on only mass spectrometry (MS) data only that proposed a set of scored/ranked structures that was validated on different types of databases and showed good results as proof of concept. Moreover, the solutions proposed by Mass Spectrometry were submitted to the prediction of NMR spectra in order to elucidate which of the proposed structures was compatible with the NMR spectra collected. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=De%20Novo" title="De Novo">De Novo</a>, <a href="https://publications.waset.org/abstracts/search?q=structure%20elucidation" title=" structure elucidation"> structure elucidation</a>, <a href="https://publications.waset.org/abstracts/search?q=mass%20spectrometry" title=" mass spectrometry"> mass spectrometry</a>, <a href="https://publications.waset.org/abstracts/search?q=NMR" title=" NMR"> NMR</a> </p> <a href="https://publications.waset.org/abstracts/58568/de-novo-structural-elucidation-from-massnmr-spectra" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/58568.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">295</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3670</span> Multiclass Analysis of Pharmaceuticals in Fish and Shrimp Tissues by High-Performance Liquid Chromatography-Tandem Mass Spectrometry</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Reza%20Pashaei">Reza Pashaei</a>, <a href="https://publications.waset.org/abstracts/search?q=Reda%20Dzingelevi%C4%8Dien%C4%97"> Reda Dzingelevičienė</a> </p> <p class="card-text"><strong>Abstract:</strong></p> An efficient, reliable, and sensitive multiclass analytical method has been expanded to simultaneously determine 15 human pharmaceutical residues in fish and shrimp tissue samples by ultra-high-performance liquid chromatography-tandem mass spectrometry. The investigated compounds comprise ten classes, namely analgesic, antibacterial, anticonvulsant, cardiovascular, fluoroquinolones, macrolides, nonsteroidal anti-inflammatory, penicillins, stimulant, and sulfonamide. A simple liquid extraction procedure based on 0.1% formic acid in methanol was developed. Chromatographic conditions were optimized, and mobile phase namely 0.1 % ammonium acetate (A), and acetonitrile (B): 0 – 2 min, 15% B; 2 – 5 min, linear to 95% B; 5 – 10 min, 95% B; and 10 – 12 min was obtained. Limits of detection and quantification ranged from 0.017 to 1.371 μg/kg and 0.051 to 4.113 μg/kg, respectively. Finally, amoxicillin, azithromycin, caffeine, carbamazepine, ciprofloxacin, clarithromycin, diclofenac, erythromycin, furosemide, ibuprofen, ketoprofen, naproxen, sulfamethoxazole, tetracycline, and triclosan were quantifiable in fish and shrimp samples. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=fish" title="fish">fish</a>, <a href="https://publications.waset.org/abstracts/search?q=liquid%20chromatography" title=" liquid chromatography"> liquid chromatography</a>, <a href="https://publications.waset.org/abstracts/search?q=mass%20spectrometry" title=" mass spectrometry"> mass spectrometry</a>, <a href="https://publications.waset.org/abstracts/search?q=pharmaceuticals" title=" pharmaceuticals"> pharmaceuticals</a>, <a href="https://publications.waset.org/abstracts/search?q=shrimp" title=" shrimp"> shrimp</a>, <a href="https://publications.waset.org/abstracts/search?q=solid-phase%20extraction" title=" solid-phase extraction"> solid-phase extraction</a> </p> <a href="https://publications.waset.org/abstracts/143257/multiclass-analysis-of-pharmaceuticals-in-fish-and-shrimp-tissues-by-high-performance-liquid-chromatography-tandem-mass-spectrometry" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/143257.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">262</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3669</span> A Method for Quantifying Arsenolipids in Sea Water by HPLC-High Resolution Mass Spectrometry </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Muslim%20Khan">Muslim Khan</a>, <a href="https://publications.waset.org/abstracts/search?q=Kenneth%20B.%20Jensen"> Kenneth B. Jensen</a>, <a href="https://publications.waset.org/abstracts/search?q=Kevin%20A.%20Francesconi"> Kevin A. Francesconi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Trace amounts (ca 1 µg/L, 13 nM) of arsenic are present in sea water mostly as the oxyanion arsenate. In contrast, arsenic is present in marine biota (animals and algae) at very high levels (up to100,000 µg/kg) a significant portion of which is present as lipid-soluble compounds collectively termed arsenolipids. The complex nature of sea water presents an analytical challenge to detect trace compounds and monitor their environmental path. We developed a simple method using liquid-liquid extraction combined with HPLC-High Resolution Mass Spectrometer capable of detecting trace of arsenolipids (99 % of the sample matrix while recovering > 80 % of the six target arsenolipids with limit of detection of 0.003 µg/L.) <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=arsenolipids" title="arsenolipids">arsenolipids</a>, <a href="https://publications.waset.org/abstracts/search?q=sea%20water" title=" sea water"> sea water</a>, <a href="https://publications.waset.org/abstracts/search?q=HPLC-high%20resolution%20mass%20spectrometry" title=" HPLC-high resolution mass spectrometry"> HPLC-high resolution mass spectrometry</a> </p> <a href="https://publications.waset.org/abstracts/39793/a-method-for-quantifying-arsenolipids-in-sea-water-by-hplc-high-resolution-mass-spectrometry" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/39793.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">366</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3668</span> Stability of Essential Oils in Pang-Rum by Gas Chromatography-Mass Spectrometry</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=K.%20Jarmkom">K. Jarmkom</a>, <a href="https://publications.waset.org/abstracts/search?q=P.%20Eakwaropas"> P. Eakwaropas</a>, <a href="https://publications.waset.org/abstracts/search?q=W.%20Khobjai"> W. Khobjai</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20Techaeoi"> S. Techaeoi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Ancient Thai perfumed powder was used as a fragrance for clothing, food, and the body. Plant-based natural Thai perfume products are known as Pang-Rum. The objective of this study was to evaluate the stability of essential oils after six months of incubation. The chemical compositions were determined by gas chromatography-mass spectrometry (GC-MS), in terms of the qualitative composition of the isolated essential oil. The isolation of the essential oil of natural products by incubate sample for 5 min at 40 ºC is described. The volatile components were identified by percentage of total peak areas comparing their retention times of GC chromatograph with NIST mass spectral library. The results show no significant difference in the seven chromatograms of perfumed powder (Pang-Rum) both with binder and without binder. Further identification was done by GC-MS. Some components of Pang-Rum with/without binder were changed by temperature and time. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=GC-MS%20analysis" title="GC-MS analysis">GC-MS analysis</a>, <a href="https://publications.waset.org/abstracts/search?q=essential%20oils" title=" essential oils"> essential oils</a>, <a href="https://publications.waset.org/abstracts/search?q=stability" title=" stability"> stability</a>, <a href="https://publications.waset.org/abstracts/search?q=Pang-Rum" title=" Pang-Rum"> Pang-Rum</a> </p> <a href="https://publications.waset.org/abstracts/61580/stability-of-essential-oils-in-pang-rum-by-gas-chromatography-mass-spectrometry" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/61580.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">272</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3667</span> Chemical Fingerprinting of Complex Samples With the Aid of Parallel Outlet Flow Chromatography</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Xavier%20A.%20Conlan">Xavier A. Conlan </a> </p> <p class="card-text"><strong>Abstract:</strong></p> Speed of analysis is a significant limitation to current high-performance liquid chromatography/mass spectrometry (HPLC/MS) and ultra-high-pressure liquid chromatography (UHPLC)/MS systems both of which are used in many forensic investigations. The flow rate limitations of MS detection require a compromise in the chromatographic flow rate, which in turn reduces throughput, and when using modern columns, a reduction in separation efficiency. Commonly, this restriction is combated through the post-column splitting of flow prior to entry into the mass spectrometer. However, this results in a loss of sensitivity and a loss in efficiency due to the post-extra column dead volume. A new chromatographic column format known as 'parallel segmented flow' involves the splitting of eluent flow within the column outlet end fitting, and in this study we present its application in order to interrogate the provenience of methamphetamine samples with mass spectrometry detection. Using parallel segmented flow, column flow rates as high as 3 mL/min were employed in the analysis of amino acids without post-column splitting to the mass spectrometer. Furthermore, when parallel segmented flow chromatography columns were employed, the sensitivity was more than twice that of conventional systems with post-column splitting when the same volume of mobile phase was passed through the detector. These finding suggest that this type of column technology will particularly enhance the capabilities of modern LC/MS enabling both high-throughput and sensitive mass spectral detection. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=chromatography" title="chromatography">chromatography</a>, <a href="https://publications.waset.org/abstracts/search?q=mass%20spectrometry%20methamphetamine" title=" mass spectrometry methamphetamine"> mass spectrometry methamphetamine</a>, <a href="https://publications.waset.org/abstracts/search?q=parallel%20segmented%20outlet%20flow%20column" title=" parallel segmented outlet flow column"> parallel segmented outlet flow column</a>, <a href="https://publications.waset.org/abstracts/search?q=forensic%20sciences" title=" forensic sciences"> forensic sciences</a> </p> <a href="https://publications.waset.org/abstracts/23798/chemical-fingerprinting-of-complex-samples-with-the-aid-of-parallel-outlet-flow-chromatography" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/23798.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">491</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3666</span> Use of Fabric Phase Sorptive Extraction with Gas Chromatography-Mass Spectrometry for the Determination of Organochlorine Pesticides in Various Aqueous and Juice Samples</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ramandeep%20Kaur">Ramandeep Kaur</a>, <a href="https://publications.waset.org/abstracts/search?q=Ashok%20Kumar%20Malik"> Ashok Kumar Malik</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Fabric Phase Sorptive Extraction (FPSE) combined with Gas chromatography Mass Spectrometry (GCMS) has been developed for the determination of nineteen organochlorine pesticides in various aqueous samples. The method consolidates the features of sol-gel derived microextraction sorbents with rich surface chemistry of cellulose fabric substrate which could directly extract sample from complex sample matrices and incredibly improve the operation with decreased pretreatment time. Some vital parameters such as kind and volume of extraction solvent and extraction time were examinedand optimized. Calibration curves were obtained in the concentration range 0.5-500 ng/mL. Under the optimum conditions, the limits of detection (LODs) were in the range 0.033 ng/mL to 0.136 ng/mL. The relative standard deviations (RSDs) for extraction of 10 ng/mL 0f OCPs were less than 10%. The developed method has been applied for the quantification of these compounds in aqueous and fruit juice samples. The results obtained proved the present method to be rapid and feasible for the determination of organochlorine pesticides in aqueous samples. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=fabric%20phase%20sorptive%20extraction" title="fabric phase sorptive extraction">fabric phase sorptive extraction</a>, <a href="https://publications.waset.org/abstracts/search?q=gas%20chromatography-mass%20spectrometry" title=" gas chromatography-mass spectrometry"> gas chromatography-mass spectrometry</a>, <a href="https://publications.waset.org/abstracts/search?q=organochlorine%20pesticides" title=" organochlorine pesticides"> organochlorine pesticides</a>, <a href="https://publications.waset.org/abstracts/search?q=sample%20pretreatment" title=" sample pretreatment"> sample pretreatment</a> </p> <a href="https://publications.waset.org/abstracts/80494/use-of-fabric-phase-sorptive-extraction-with-gas-chromatography-mass-spectrometry-for-the-determination-of-organochlorine-pesticides-in-various-aqueous-and-juice-samples" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/80494.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">484</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3665</span> Comprehending the Relationship between the Red Blood Cells of a Protein 4.1 -/- Patient and Those of Healthy Controls: A Comprehensive Analysis of Tandem Mass Spectrometry Data</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ahmed%20M.%20Hjazi">Ahmed M. Hjazi</a>, <a href="https://publications.waset.org/abstracts/search?q=Bader%20M.%20Hjazi"> Bader M. Hjazi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Protein 4.1 is a crucial component of complex interactions between the cytoskeleton and other junctional complex proteins. When the gene encoding this protein is altered, resulting in reduced expression, or when the protein is absent, the red cell undergoes a significant structural change. This research aims to achieve a deeper comprehension of the biochemical effects of red cell protein deficiency. A Tandem Mass Spectrometry Analysis (TMT-MS/MS) of patient cells lacking protein 4.1 compared to three healthy controls was achieved by the Proteomics Institute of the University of Bristol. The SDS-PAGE and Western blotting were utilized on the original patient sample and controls to partially confirm TMT MS/MS data analysis of the protein-4.1-deficient cells. Compared to healthy controls, protein levels in samples lacking protein 4.1 had a significantly higher concentration of proteins that probably originated from reticulocytes. This could occur if the patient has an elevated reticulocyte count. The increase in chaperone and reticulocyte-associated proteins was most notable in this study. This may result from elevated quantities of reticulocytes in patients with hereditary elliptocytosis. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=hereditary%20elliptocytosis" title="hereditary elliptocytosis">hereditary elliptocytosis</a>, <a href="https://publications.waset.org/abstracts/search?q=protein%204.1" title=" protein 4.1"> protein 4.1</a>, <a href="https://publications.waset.org/abstracts/search?q=red%20cells" title=" red cells"> red cells</a>, <a href="https://publications.waset.org/abstracts/search?q=tandem%20mass%20spectrometry%20data." title=" tandem mass spectrometry data."> tandem mass spectrometry data.</a> </p> <a href="https://publications.waset.org/abstracts/165174/comprehending-the-relationship-between-the-red-blood-cells-of-a-protein-41-patient-and-those-of-healthy-controls-a-comprehensive-analysis-of-tandem-mass-spectrometry-data" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/165174.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">79</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3664</span> Urinary Volatile Organic Compound Testing in Fast-Track Patients with Suspected Colorectal Cancer</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Godwin%20Dennison">Godwin Dennison</a>, <a href="https://publications.waset.org/abstracts/search?q=C.%20E.%20Boulind"> C. E. Boulind</a>, <a href="https://publications.waset.org/abstracts/search?q=O.%20Gould"> O. Gould</a>, <a href="https://publications.waset.org/abstracts/search?q=B.%20de%20Lacy%20Costello"> B. de Lacy Costello</a>, <a href="https://publications.waset.org/abstracts/search?q=J.%20Allison"> J. Allison</a>, <a href="https://publications.waset.org/abstracts/search?q=P.%20White"> P. White</a>, <a href="https://publications.waset.org/abstracts/search?q=P.%20Ewings"> P. Ewings</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20Wicaksono"> A. Wicaksono</a>, <a href="https://publications.waset.org/abstracts/search?q=N.%20J.%20Curtis"> N. J. Curtis</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20Pullyblank"> A. Pullyblank</a>, <a href="https://publications.waset.org/abstracts/search?q=D.%20Jayne"> D. Jayne</a>, <a href="https://publications.waset.org/abstracts/search?q=J.%20A.%20Covington"> J. A. Covington</a>, <a href="https://publications.waset.org/abstracts/search?q=N.%20Ratcliffe"> N. Ratcliffe</a>, <a href="https://publications.waset.org/abstracts/search?q=N.%20K.%20Francis"> N. K. Francis</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: Colorectal symptoms are common but only infrequently represent serious pathology, including colorectal cancer (CRC). A large number of invasive tests are presently performed for reassurance. We investigated the feasibility of urinary volatile organic compound (VOC) testing as a potential triage tool in patients fast-tracked for assessment for possible CRC. Methods: A prospective, multi-centre, observational feasibility study was performed across three sites. Patients referred on NHS fast-track pathways for potential CRC provided a urine sample which underwent Gas Chromatography Mass Spectrometry (GC-MS), Field Asymmetric Ion Mobility Spectrometry (FAIMS) and Selected Ion Flow Tube Mass Spectrometry (SIFT-MS) analysis. Patients underwent colonoscopy and/or CT colonography and were grouped as either CRC, adenomatous polyp(s), or controls to explore the diagnostic accuracy of VOC output data supported by an artificial neural network (ANN) model. Results: 558 patients participated with 23 (4.1%) CRC diagnosed. 59% of colonoscopies and 86% of CT colonographies showed no abnormalities. Urinary VOC testing was feasible, acceptable to patients, and applicable within the clinical fast track pathway. GC-MS showed the highest clinical utility for CRC and polyp detection vs. controls (sensitivity=0.878, specificity=0.882, AUROC=0.884). Conclusion: Urinary VOC testing and analysis are feasible within NHS fast-track CRC pathways. Clinically meaningful differences between patients with cancer, polyps, or no pathology were identified therefore suggesting VOC analysis may have future utility as a triage tool. Acknowledgment: Funding: NIHR Research for Patient Benefit grant (ref: PB-PG-0416-20022). <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=colorectal%20cancer" title="colorectal cancer">colorectal cancer</a>, <a href="https://publications.waset.org/abstracts/search?q=volatile%20organic%20compound" title=" volatile organic compound"> volatile organic compound</a>, <a href="https://publications.waset.org/abstracts/search?q=gas%20chromatography%20mass%20spectrometry" title=" gas chromatography mass spectrometry"> gas chromatography mass spectrometry</a>, <a href="https://publications.waset.org/abstracts/search?q=field%20asymmetric%20ion%20mobility%20spectrometry" title=" field asymmetric ion mobility spectrometry"> field asymmetric ion mobility spectrometry</a>, <a href="https://publications.waset.org/abstracts/search?q=selected%20ion%20flow%20tube%20mass%20spectrometry" title=" selected ion flow tube mass spectrometry"> selected ion flow tube mass spectrometry</a> </p> <a href="https://publications.waset.org/abstracts/152812/urinary-volatile-organic-compound-testing-in-fast-track-patients-with-suspected-colorectal-cancer" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/152812.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">91</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3663</span> Structural Elucidation of Intact Rough-Type Lipopolysaccharides using Field Asymmetric Ion Mobility Spectrometry and Kendrick Mass Defect Plots</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Abanoub%20Mikhael">Abanoub Mikhael</a>, <a href="https://publications.waset.org/abstracts/search?q=Darryl%20Hardie"> Darryl Hardie</a>, <a href="https://publications.waset.org/abstracts/search?q=Derek%20Smith"> Derek Smith</a>, <a href="https://publications.waset.org/abstracts/search?q=Helena%20Petrosova"> Helena Petrosova</a>, <a href="https://publications.waset.org/abstracts/search?q=Robert%20Ernst"> Robert Ernst</a>, <a href="https://publications.waset.org/abstracts/search?q=David%20Goodlett"> David Goodlett</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Lipopolysaccharide (LPS) is a hallmark virulence factor of Gram-negative bacteria. It is a complex, structurally het- erogeneous mixture due to variations in number, type, and position of its simplest units: fatty acids and monosaccharides. Thus, LPS structural characterization by traditional mass spectrometry (MS) methods is challenging. Here, we describe the benefits of field asymmetric ion mobility spectrometry (FAIMS) for analysis of intact R-type lipopolysaccharide complex mixture (lipooligo- saccharide; LOS). Structural characterization was performed using Escherichia coli J5 (Rc mutant) LOS, a TLR4 agonist widely used in glycoconjugate vaccine research. FAIMS gas phase fractionation improved the (S/N) ratio and number of detected LOS species. Additionally, FAIMS allowed the separation of overlapping isobars facilitating their tandem MS characterization and un- equivocal structural assignments. In addition to FAIMS gas phase fractionation benefits, extra sorting of the structurally related LOS molecules was further accomplished using Kendrick mass defect (KMD) plots. Notably, a custom KMD base unit of [Na-H] created a highly organized KMD plot that allowed identification of interesting and novel structural differences across the different LOS ion families, i.e., ions with different acylation degrees, oligosaccharides composition, and chemical modifications. Defining the composition of a single LOS ion by tandem MS along with the organized KMD plot structural network was sufficient to deduce the composition of 181 LOS species out of 321 species present in the mixture. The combination of FAIMS and KMD plots allowed in-depth characterization of the complex LOS mixture and uncovered a wealth of novel information about its structural variations. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=lipopolysaccharide" title="lipopolysaccharide">lipopolysaccharide</a>, <a href="https://publications.waset.org/abstracts/search?q=ion%20mobility%20MS" title=" ion mobility MS"> ion mobility MS</a>, <a href="https://publications.waset.org/abstracts/search?q=Kendrick%20mass%20defect" title=" Kendrick mass defect"> Kendrick mass defect</a>, <a href="https://publications.waset.org/abstracts/search?q=Tandem%20mass%20spectrometry" title=" Tandem mass spectrometry"> Tandem mass spectrometry</a> </p> <a href="https://publications.waset.org/abstracts/173086/structural-elucidation-of-intact-rough-type-lipopolysaccharides-using-field-asymmetric-ion-mobility-spectrometry-and-kendrick-mass-defect-plots" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/173086.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">71</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3662</span> A Turn-on Fluorescent Sensor for Pb(II)</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ece%20K%C3%B6k%20Yetimo%C4%9Flu">Ece Kök Yetimoğlu</a>, <a href="https://publications.waset.org/abstracts/search?q=Soner%20%C3%87ubuk"> Soner Çubuk</a>, <a href="https://publications.waset.org/abstracts/search?q=Ne%C5%9Fe%20Ta%C5%9Fci"> Neşe Taşci</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Vezir%20Kahraman"> M. Vezir Kahraman</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Lead(II) is one of the most toxic environmental pollutants in the world, due to its high toxicity and non-biodegradability. Lead exposure causes severe risks to human health such as central brain damages, convulsions, kidney damages, and even death. To determine lead(II) in environmental or biological samples, scientists use atomic absorption spectrometry (AAS), inductively coupled plasma mass spectrometry (ICPMS), fluorescence spectrometry and electrochemical techniques. Among these systems the fluorescence spectrometry and fluorescent chemical sensors have attracted considerable attention because of their good selectivity and high sensitivity. The fluorescent polymers usually contain covalently bonded fluorophores. In this study imidazole based UV cured polymeric film was prepared and designed to act as a fluorescence chemo sensor for lead (II) analysis. The optimum conditions such as influence of pH value and time on the fluorescence intensity of the sensor have also been investigated. The sensor was highly sensitive with a detection limit as low as 1.87 × 10−8 mol L-1 and it was successful in the determination of Pb(II) in water samples. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=fluorescence" title="fluorescence">fluorescence</a>, <a href="https://publications.waset.org/abstracts/search?q=lead%28II%29" title=" lead(II)"> lead(II)</a>, <a href="https://publications.waset.org/abstracts/search?q=photopolymerization" title=" photopolymerization"> photopolymerization</a>, <a href="https://publications.waset.org/abstracts/search?q=polymeric%20sensor" title=" polymeric sensor"> polymeric sensor</a> </p> <a href="https://publications.waset.org/abstracts/46887/a-turn-on-fluorescent-sensor-for-pbii" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/46887.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">671</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3661</span> Deformulation and Comparative Analysis of Apparently Similar Polymers Using Multiple Modes of Pyrolysis-Gc/Ms</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Athena%20Nguyen">Athena Nguyen</a>, <a href="https://publications.waset.org/abstracts/search?q=Rojin%20Belganeh"> Rojin Belganeh</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Detecting and identifying differences in like polymer materials are key factors in deformulation, comparative analysis as well as reverse engineering. Pyrolysis-GC/MS is an easy solid sample introduction technique which expands the application areas of gas chromatography and mass spectrometry. The Micro-furnace pyrolyzer is directly interfaced with the GC injector preventing any potential of cold spot, carryover, and cross contamination. This presentation demonstrates the study of two similar polymers by performing different mode of operations in the same system: Evolve gas analysis (EGA), Flash pyrolysis, Thermal desorption analysis, and Heart-cutting analysis. Unknown polymer materials and their chemical compositions are identified. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=gas%20chromatography%2Fmass%20spectrometry" title="gas chromatography/mass spectrometry">gas chromatography/mass spectrometry</a>, <a href="https://publications.waset.org/abstracts/search?q=pyrolysis" title=" pyrolysis"> pyrolysis</a>, <a href="https://publications.waset.org/abstracts/search?q=pyrolyzer" title=" pyrolyzer"> pyrolyzer</a>, <a href="https://publications.waset.org/abstracts/search?q=thermal%20desorption-GC%2FMS" title=" thermal desorption-GC/MS"> thermal desorption-GC/MS</a> </p> <a href="https://publications.waset.org/abstracts/139719/deformulation-and-comparative-analysis-of-apparently-similar-polymers-using-multiple-modes-of-pyrolysis-gcms" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/139719.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">264</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3660</span> Heroin and Opiates Metabolites Tracing by Gas-Chromatography Isotope Ratio Mass Spectrometry</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Yao-Te%20Yen">Yao-Te Yen</a>, <a href="https://publications.waset.org/abstracts/search?q=Chao-Hsin%20Cheng"> Chao-Hsin Cheng</a>, <a href="https://publications.waset.org/abstracts/search?q=Meng-Shun%20Huang"> Meng-Shun Huang</a>, <a href="https://publications.waset.org/abstracts/search?q=Shan-Zong%20Cyue"> Shan-Zong Cyue</a> </p> <p class="card-text"><strong>Abstract:</strong></p> 'Poppy-seed defense' has been a serious problem all over the world, that is because the opiates metabolites in urine are difficult to distinguish where they come from precisely. In this research, a powerful analytic method has been developed to trace the opiates metabolites in urine by Gas-Chromatography Isotope Ratio Mass Spectrometry (GC-IRMS). In order to eliminate the interference of synthesis to heroin or metabolism through human body, opiates metabolites in urine and sized heroin were hydrolyzed to morphine. Morphine is the key compound for tracing between opiates metabolites and seized heroin in this research. By matching δ13C and δ15N values through morphine, it is successful to distinguish the opiates metabolites coming from heroin or medicine. We tested seven heroin abuser’s metabolites and seized heroin in crime sites, the result showed that opiates metabolites coming from seized heroin, the variation of δ13C and δ15N for morphine are within 0.2 and 2.5‰, respectively. The variation of δ13C and δ15N for morphine are reasonable with the result of matrix match experiments. Above all, the uncertainty of 'Poppy-seed defense' can be solved easily by this analytic method, it provides the direct evidence for judge to make accurate conviction without hesitation. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=poppy-seed%20defense" title="poppy-seed defense">poppy-seed defense</a>, <a href="https://publications.waset.org/abstracts/search?q=heroin" title=" heroin"> heroin</a>, <a href="https://publications.waset.org/abstracts/search?q=opiates%20metabolites" title=" opiates metabolites"> opiates metabolites</a>, <a href="https://publications.waset.org/abstracts/search?q=isotope%20ratio%20mass%20spectrometry" title=" isotope ratio mass spectrometry"> isotope ratio mass spectrometry</a> </p> <a href="https://publications.waset.org/abstracts/74236/heroin-and-opiates-metabolites-tracing-by-gas-chromatography-isotope-ratio-mass-spectrometry" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/74236.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">239</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3659</span> Aflatoxins Characterization in Remedial Plant-Delphinium denudatum by High-Performance Liquid Chromatography–Tandem Mass Spectrometry</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Nadeem%20A.%20Siddique">Nadeem A. Siddique</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohd%20Mujeeb"> Mohd Mujeeb</a>, <a href="https://publications.waset.org/abstracts/search?q=Kahkashan"> Kahkashan</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Introduction: The objective of the projected work is to study the occurrence of the aflatoxins B1, B2, G1and G2 in remedial plants, exclusively in Delphinium denudatum. The aflatoxins were analysed by high-performance liquid chromatography–tandem quadrupole mass spectrometry with electrospray ionization (HPLC–MS/MS) and immunoaffinity column chromatography were used for extraction and purification of aflatoxins. PDA media was selected for fungal count. Results: A good quality linear relationship was originated for AFB1, AFB2, AFG1 and AFG2 at 1–10 ppb (r > 0.9995). The analyte precision at three different spiking levels was 88.7–109.1 %, by means of low per cent relative standard deviations in each case. Within 5 to7 min aflatoxins can be separated using an Agilent XDB C18-column. We found that AFB1 and AFB2 were not found in D. denudatum. This was reliable through exceptionally low figures of fungal colonies observed after 6 hr of incubation. The developed analytical method is straightforward, be successfully used to determine the aflatoxins. Conclusion: The developed analytical method is straightforward, simple, accurate, economical and can be successfully used to find out the aflatoxins in remedial plants and consequently to have power over the quality of products. The presence of aflatoxin in the plant extracts was interrelated to the least fungal load in the remedial plants examined. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=aflatoxins" title="aflatoxins">aflatoxins</a>, <a href="https://publications.waset.org/abstracts/search?q=delphinium%20denudatum" title=" delphinium denudatum"> delphinium denudatum</a>, <a href="https://publications.waset.org/abstracts/search?q=liquid%20chromatography" title=" liquid chromatography"> liquid chromatography</a>, <a href="https://publications.waset.org/abstracts/search?q=mass%20spectrometry" title=" mass spectrometry"> mass spectrometry</a> </p> <a href="https://publications.waset.org/abstracts/56463/aflatoxins-characterization-in-remedial-plant-delphinium-denudatum-by-high-performance-liquid-chromatography-tandem-mass-spectrometry" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/56463.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">213</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3658</span> Automatic Threshold Search for Heat Map Based Feature Selection: A Cancer Dataset Analysis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Carlos%20Huertas">Carlos Huertas</a>, <a href="https://publications.waset.org/abstracts/search?q=Reyes%20Juarez-Ramirez"> Reyes Juarez-Ramirez</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Public health is one of the most critical issues today; therefore, there is great interest to improve technologies in the area of diseases detection. With machine learning and feature selection, it has been possible to aid the diagnosis of several diseases such as cancer. In this work, we present an extension to the Heat Map Based Feature Selection algorithm, this modification allows automatic threshold parameter selection that helps to improve the generalization performance of high dimensional data such as mass spectrometry. We have performed a comparison analysis using multiple cancer datasets and compare against the well known Recursive Feature Elimination algorithm and our original proposal, the results show improved classification performance that is very competitive against current techniques. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=biomarker%20discovery" title="biomarker discovery">biomarker discovery</a>, <a href="https://publications.waset.org/abstracts/search?q=cancer" title=" cancer"> cancer</a>, <a href="https://publications.waset.org/abstracts/search?q=feature%20selection" title=" feature selection"> feature selection</a>, <a href="https://publications.waset.org/abstracts/search?q=mass%20spectrometry" title=" mass spectrometry"> mass spectrometry</a> </p> <a href="https://publications.waset.org/abstracts/46310/automatic-threshold-search-for-heat-map-based-feature-selection-a-cancer-dataset-analysis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/46310.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">338</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3657</span> Determination of Micronutrients in the Fruit of Cydonia oblonga Miller</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Madrakhimova%20Sakhiba">Madrakhimova Sakhiba</a>, <a href="https://publications.waset.org/abstracts/search?q=Matmurotov%20Bakhtishod"> Matmurotov Bakhtishod</a>, <a href="https://publications.waset.org/abstracts/search?q=Boltaboyava%20Zilola"> Boltaboyava Zilola</a>, <a href="https://publications.waset.org/abstracts/search?q=Matchanov%20Alimjan"> Matchanov Alimjan</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Analyzing the chemical composition of locally consumed food products is one of the urgent problems in the health sector today. Taking this into account, it analyzed the microelement content of Cydonia oblonga Miller (COM) fruit growing in the Republic of Uzbekistan using the ISP MS inductively coupled mass spectrometry method. fruits brought to a constant mass in the analysis were mineralized in a mixture of nitric acid-HNO₃ and hydrogen peroxide-H₂O₂ in a ratio of 3:2. The mineralized extract was diluted to 50 milliliters with double-distilled water and analyzed. The results of the analysis showed that the fruit is rich in micronutrients necessary for the human body, especially potassium-K and phosphorus-P among macroelements, Strontium-Sr and barium-Ba from microelements are more than other microelements. It was observed that the amount of trace elements contained in COM fruit does not exceed the permissible standards. Therefore, it can be recommended to eat this fruit every day to prevent various diseases that occur in the human body. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cydonia%20oblonga%20miller" title="cydonia oblonga miller">cydonia oblonga miller</a>, <a href="https://publications.waset.org/abstracts/search?q=macroelement" title=" macroelement"> macroelement</a>, <a href="https://publications.waset.org/abstracts/search?q=microelement" title=" microelement"> microelement</a>, <a href="https://publications.waset.org/abstracts/search?q=inductively%20coupled%20mass%20spectrometry" title=" inductively coupled mass spectrometry"> inductively coupled mass spectrometry</a>, <a href="https://publications.waset.org/abstracts/search?q=hydrolysis" title=" hydrolysis"> hydrolysis</a>, <a href="https://publications.waset.org/abstracts/search?q=mineralization" title=" mineralization"> mineralization</a> </p> <a href="https://publications.waset.org/abstracts/181523/determination-of-micronutrients-in-the-fruit-of-cydonia-oblonga-miller" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/181523.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">71</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3656</span> Dairy Wastewater Treatment by Electrochemical and Catalytic Method</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Basanti%20Ekka">Basanti Ekka</a>, <a href="https://publications.waset.org/abstracts/search?q=Talis%20Juhna"> Talis Juhna </a> </p> <p class="card-text"><strong>Abstract:</strong></p> Dairy industrial effluents originated by the typical processing activities are composed of various organic and inorganic constituents, and these include proteins, fats, inorganic salts, antibiotics, detergents, sanitizers, pathogenic viruses, bacteria, etc. These contaminants are harmful to not only human beings but also aquatic flora and fauna. Because consisting of large classes of contaminants, the specific targeted removal methods available in the literature are not viable solutions on the industrial scale. Therefore, in this on-going research, a series of coagulation, electrochemical, and catalytic methods will be employed. The bulk coagulation and electrochemical methods can wash off most of the contaminants, but some of the harmful chemicals may slip in; therefore, specific catalysts designed and synthesized will be employed for the removal of targeted chemicals. In the context of Latvian dairy industries, presently, work is under progress on the characterization of dairy effluents by total organic carbon (TOC), Inductively Coupled Plasma Mass Spectrometry (ICP-MS)/ Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES), High-Performance Liquid Chromatography (HPLC), Gas Chromatography-Mass Spectrometry (GC-MS), and Mass Spectrometry. After careful evaluation of the dairy effluents, a cost-effective natural coagulant will be employed prior to advanced electrochemical technology such as electrocoagulation and electro-oxidation as a secondary treatment process. Finally, graphene oxide (GO) based hybrid materials will be used for post-treatment of dairy wastewater as graphene oxide has been widely applied in various fields such as environmental remediation and energy production due to the presence of various oxygen-containing groups. Modified GO will be used as a catalyst for the removal of remaining contaminants after the electrochemical process. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=catalysis" title="catalysis">catalysis</a>, <a href="https://publications.waset.org/abstracts/search?q=dairy%20wastewater" title=" dairy wastewater"> dairy wastewater</a>, <a href="https://publications.waset.org/abstracts/search?q=electrochemical%20method" title=" electrochemical method"> electrochemical method</a>, <a href="https://publications.waset.org/abstracts/search?q=graphene%20oxide" title=" graphene oxide"> graphene oxide</a> </p> <a href="https://publications.waset.org/abstracts/111262/dairy-wastewater-treatment-by-electrochemical-and-catalytic-method" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/111262.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">144</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3655</span> Potential Biosorption of Rhodococcus erythropolis, an Isolated Strain from Sossego Copper Mine, Brazil </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Marcela%20dos%20P.%20G.%20Baltazar">Marcela dos P. G. Baltazar</a>, <a href="https://publications.waset.org/abstracts/search?q=Louise%20H.%20Gracioso"> Louise H. Gracioso</a>, <a href="https://publications.waset.org/abstracts/search?q=Luciana%20J.%20Gimenes"> Luciana J. Gimenes</a>, <a href="https://publications.waset.org/abstracts/search?q=Bruno%20Karolski"> Bruno Karolski</a>, <a href="https://publications.waset.org/abstracts/search?q=Ingrid%20Avanzi"> Ingrid Avanzi</a>, <a href="https://publications.waset.org/abstracts/search?q=Elen%20A.%20Perpetuo"> Elen A. Perpetuo</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In this work, bacterial strains were isolated from environmental samples from a copper mine and three of them presented potential for bioremediation of copper. All the strains were identified by mass spectrometry (MALDI-TOF-Biotyper) and grown in three diferent media supplemented with 100 ppm of copper chloride in flasks of 500mL and it was incubated at 28 °C and 180 rpm. Periodically, samples were taken and monitored for cellular growth and copper biosorption by spectrophotometer UV-Vis (600 nm) and Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES), respectively. At the end of exponential phase of cellular growth, the biomass was utilized to construct a correlation curve between absorbance and dry mass of the cells. Among the three isolates with potential for biorremediation, 1 strain exhibit capacity the most for bioremediation of effluents contaminated by copper being identified as Rhodococcus erythropolis. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bioprocess" title="bioprocess">bioprocess</a>, <a href="https://publications.waset.org/abstracts/search?q=bioremediation" title=" bioremediation"> bioremediation</a>, <a href="https://publications.waset.org/abstracts/search?q=biosorption" title=" biosorption"> biosorption</a>, <a href="https://publications.waset.org/abstracts/search?q=copper" title=" copper"> copper</a> </p> <a href="https://publications.waset.org/abstracts/30539/potential-biosorption-of-rhodococcus-erythropolis-an-isolated-strain-from-sossego-copper-mine-brazil" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/30539.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">388</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3654</span> Establishing a Computational Screening Framework to Identify Environmental Exposures Using Untargeted Gas-Chromatography High-Resolution Mass Spectrometry</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Juni%20C.%20Kim">Juni C. Kim</a>, <a href="https://publications.waset.org/abstracts/search?q=Anna%20R.%20Robuck"> Anna R. Robuck</a>, <a href="https://publications.waset.org/abstracts/search?q=Douglas%20I.%20Walker"> Douglas I. Walker</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The human exposome, which includes chemical exposures over the lifetime and their effects, is now recognized as an important measure for understanding human health; however, the complexity of the data makes the identification of environmental chemicals challenging. The goal of our project was to establish a computational workflow for the improved identification of environmental pollutants containing chlorine or bromine. Using the “pattern. search” function available in the R package NonTarget, we wrote a multifunctional script that searches mass spectral clusters from untargeted gas-chromatography high-resolution mass spectrometry (GC-HRMS) for the presence of spectra consistent with chlorine and bromine-containing organic compounds. The “pattern. search” function was incorporated into a different function that allows the evaluation of clusters containing multiple analyte fragments, has multi-core support, and provides a simplified output identifying listing compounds containing chlorine and/or bromine. The new function was able to process 46,000 spectral clusters in under 8 seconds and identified over 150 potential halogenated spectra. We next applied our function to a deidentified dataset from patients diagnosed with primary biliary cholangitis (PBC), primary sclerosing cholangitis (PSC), and healthy controls. Twenty-two spectra corresponded to potential halogenated compounds in the PSC and PBC dataset, including six significantly different in PBC patients, while four differed in PSC patients. We have developed an improved algorithm for detecting halogenated compounds in GC-HRMS data, providing a strategy for prioritizing exposures in the study of human disease. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=exposome" title="exposome">exposome</a>, <a href="https://publications.waset.org/abstracts/search?q=metabolome" title=" metabolome"> metabolome</a>, <a href="https://publications.waset.org/abstracts/search?q=computational%20metabolomics" title=" computational metabolomics"> computational metabolomics</a>, <a href="https://publications.waset.org/abstracts/search?q=high-resolution%20mass%20spectrometry" title=" high-resolution mass spectrometry"> high-resolution mass spectrometry</a>, <a href="https://publications.waset.org/abstracts/search?q=exposure" title=" exposure"> exposure</a>, <a href="https://publications.waset.org/abstracts/search?q=pollutants" title=" pollutants"> pollutants</a> </p> <a href="https://publications.waset.org/abstracts/157128/establishing-a-computational-screening-framework-to-identify-environmental-exposures-using-untargeted-gas-chromatography-high-resolution-mass-spectrometry" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/157128.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">138</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3653</span> Plasma-Induced Modification of Biomolecules: A Tool for Analysis of Protein Structures</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Yuting%20Wu">Yuting Wu</a>, <a href="https://publications.waset.org/abstracts/search?q=Faraz%20Choudhury"> Faraz Choudhury</a>, <a href="https://publications.waset.org/abstracts/search?q=Daniel%20Benjamin"> Daniel Benjamin</a>, <a href="https://publications.waset.org/abstracts/search?q=James%20Whalin"> James Whalin</a>, <a href="https://publications.waset.org/abstracts/search?q=Joshua%20Blatz"> Joshua Blatz</a>, <a href="https://publications.waset.org/abstracts/search?q=Leon%20Shohet"> Leon Shohet</a>, <a href="https://publications.waset.org/abstracts/search?q=Michael%20Sussman"> Michael Sussman</a>, <a href="https://publications.waset.org/abstracts/search?q=Mark%20Richards"> Mark Richards</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Plasma-Induced Modification of Biomolecules (PLIMB) has been developed as a technology, which, together with mass spectrometry, measures three-dimensional structural characteristics of proteins. This technique uses hydroxyl radicals generated by atmospheric-pressure plasma discharge to react with the solvent-accessible side chains of protein in an aqueous solution. In this work, we investigate the three-dimensional structure of hemoglobin and myoglobin using PLIMB. Additional modifications to these proteins, such as oxidation, fragmentations, and conformational changes caused by PLIMB are also explored. These results show that PLIMB, coupled with mass spectrometry, is an effective way to determine solvent access to hemoproteins. Furthermore, we show that many factors, including pH and the electrical parameters used to generate the plasma, have a significant influence on solvent accessibility. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=plasma" title="plasma">plasma</a>, <a href="https://publications.waset.org/abstracts/search?q=hemoglobin" title=" hemoglobin"> hemoglobin</a>, <a href="https://publications.waset.org/abstracts/search?q=myoglobin" title=" myoglobin"> myoglobin</a>, <a href="https://publications.waset.org/abstracts/search?q=solvent%20access" title=" solvent access"> solvent access</a> </p> <a href="https://publications.waset.org/abstracts/124966/plasma-induced-modification-of-biomolecules-a-tool-for-analysis-of-protein-structures" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/124966.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">193</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3652</span> Body Fluids Identification by Raman Spectroscopy and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Huixia%20Shi">Huixia Shi</a>, <a href="https://publications.waset.org/abstracts/search?q=Can%20Hu"> Can Hu</a>, <a href="https://publications.waset.org/abstracts/search?q=Jun%20Zhu"> Jun Zhu</a>, <a href="https://publications.waset.org/abstracts/search?q=Hongling%20Guo"> Hongling Guo</a>, <a href="https://publications.waset.org/abstracts/search?q=Haiyan%20Li"> Haiyan Li</a>, <a href="https://publications.waset.org/abstracts/search?q=Hongyan%20Du"> Hongyan Du</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The identification of human body fluids during forensic investigations is a critical step to determine key details, and present strong evidence to testify criminal in a case. With the popularity of DNA and improved detection technology, the potential question must be revolved that whether the suspect’s DNA derived from saliva or semen, menstrual or peripheral blood, how to identify the red substance or aged blood traces on the spot is blood; How to determine who contribute the right one in mixed stains. In recent years, molecular approaches have been developing increasingly on mRNA, miRNA, DNA methylation and microbial markers, but appear expensive, time-consuming, and destructive disadvantages. Physicochemical methods are utilized frequently such us scanning electron microscopy/energy spectroscopy and X-ray fluorescence and so on, but results only showing one or two characteristics of body fluid itself and that out of working in unknown or mixed body fluid stains. This paper focuses on using chemistry methods Raman spectroscopy and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry to discriminate species of peripheral blood, menstrual blood, semen, saliva, vaginal secretions, urine or sweat. Firstly, non-destructive, confirmatory, convenient and fast Raman spectroscopy method combined with more accurate matrix-assisted laser desorption/ionization time-of-flight mass spectrometry method can totally distinguish one from other body fluids. Secondly, 11 spectral signatures and specific metabolic molecules have been obtained by analysis results after 70 samples detected. Thirdly, Raman results showed peripheral and menstrual blood, saliva and vaginal have highly similar spectroscopic features. Advanced statistical analysis of the multiple Raman spectra must be requested to classify one to another. On the other hand, it seems that the lactic acid can differentiate peripheral and menstrual blood detected by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, but that is not a specific metabolic molecule, more sensitivity ones will be analyzed in a forward study. These results demonstrate the great potential of the developed chemistry methods for forensic applications, although more work is needed for method validation. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=body%20fluids" title="body fluids">body fluids</a>, <a href="https://publications.waset.org/abstracts/search?q=identification" title=" identification"> identification</a>, <a href="https://publications.waset.org/abstracts/search?q=Raman%20spectroscopy" title=" Raman spectroscopy"> Raman spectroscopy</a>, <a href="https://publications.waset.org/abstracts/search?q=matrix-assisted%20laser%20desorption%2Fionization%20time-of-flight%20mass%20spectrometry" title=" matrix-assisted laser desorption/ionization time-of-flight mass spectrometry"> matrix-assisted laser desorption/ionization time-of-flight mass spectrometry</a> </p> <a href="https://publications.waset.org/abstracts/103873/body-fluids-identification-by-raman-spectroscopy-and-matrix-assisted-laser-desorptionionization-time-of-flight-mass-spectrometry" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/103873.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">137</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3651</span> Analysis of the Volatile Organic Compounds of Tillandsia Flowers by HS-SPME/GC-MS</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Alexandre%20Gonzalez">Alexandre Gonzalez</a>, <a href="https://publications.waset.org/abstracts/search?q=Zohra%20Benfodda"> Zohra Benfodda</a>, <a href="https://publications.waset.org/abstracts/search?q=David%20B%C3%A9nim%C3%A9lis"> David Bénimélis</a>, <a href="https://publications.waset.org/abstracts/search?q=Jean-Xavier%20Fontaine"> Jean-Xavier Fontaine</a>, <a href="https://publications.waset.org/abstracts/search?q=Roland%20Molini%C3%A9"> Roland Molinié</a>, <a href="https://publications.waset.org/abstracts/search?q=Patrick%20Meffre"> Patrick Meffre</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Volatile organic compounds (VOCs) emitted by flowers play an important role in plant ecology. However, the Tillandsia genus has been scarcely studied according to the VOCs emitted by flowers. Tillandsia are epiphytic flowering plants belonging to the Bromeliaceae family. The VOCs composition of twelve unscented and two faint-scented Tillandsia species was studied. The headspace solid phase microextraction coupled with gas chromatography combined with mass spectrometry method was used to explore the chemical diversity of the VOCs. This study allowed the identification of 65 VOCs among the fourteen species, and between six to twenty-five compounds were identified in each of the species. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=tillandsia" title="tillandsia">tillandsia</a>, <a href="https://publications.waset.org/abstracts/search?q=headspace%20solid%20phase%20microextraction%20%28HS-SPME%29" title=" headspace solid phase microextraction (HS-SPME)"> headspace solid phase microextraction (HS-SPME)</a>, <a href="https://publications.waset.org/abstracts/search?q=gas%20chromatography-mass%20spectrometry%20%28GC-MS%29" title=" gas chromatography-mass spectrometry (GC-MS)"> gas chromatography-mass spectrometry (GC-MS)</a>, <a href="https://publications.waset.org/abstracts/search?q=scentless%20flowers" title=" scentless flowers"> scentless flowers</a>, <a href="https://publications.waset.org/abstracts/search?q=volatile%20organic%20compounds%20%28VOCs%29" title=" volatile organic compounds (VOCs)"> volatile organic compounds (VOCs)</a>, <a href="https://publications.waset.org/abstracts/search?q=PCA%20analysis" title=" PCA analysis"> PCA analysis</a>, <a href="https://publications.waset.org/abstracts/search?q=heatmap" title=" heatmap"> heatmap</a> </p> <a href="https://publications.waset.org/abstracts/152016/analysis-of-the-volatile-organic-compounds-of-tillandsia-flowers-by-hs-spmegc-ms" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/152016.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">124</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3650</span> Determination of a Novel Artificial Sweetener Advantame in Food by Liquid Chromatography Tandem Mass Spectrometry</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Fangyan%20Li">Fangyan Li</a>, <a href="https://publications.waset.org/abstracts/search?q=Lin%20Min%20Lee"> Lin Min Lee</a>, <a href="https://publications.waset.org/abstracts/search?q=Hui%20Zhu%20Peh"> Hui Zhu Peh</a>, <a href="https://publications.waset.org/abstracts/search?q=Shoet%20Harn%20Chan"> Shoet Harn Chan</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Advantame, a derivative of aspartame, is the latest addition to a family of low caloric and high potent dipeptide sweeteners which include aspartame, neotame and alitame. The use of advantame as a high-intensity sweetener in food was first accepted by Food Standards Australia New Zealand in 2011 and subsequently by US and EU food authorities in 2014, with the results from toxicity and exposure studies showing advantame poses no safety concern to the public at regulated levels. To our knowledge, currently there is barely any detailed information on the analytical method of advantame in food matrix, except for one report published in Japanese, stating a high performance liquid chromatography (HPLC) and liquid chromatography/ mass spectrometry (LC-MS) method with a detection limit at ppm level. However, the use of acid in sample preparation and instrumental analysis in the report raised doubt over the reliability of the method, as there is indication that stability of advantame is compromised under acidic conditions. Besides, the method may not be suitable for analyzing food matrices containing advantame at low ppm or sub-ppm level. In this presentation, a simple, specific and sensitive method for the determination of advantame in food is described. The method involved extraction with water and clean-up via solid phase extraction (SPE) followed by detection using liquid chromatography tandem mass spectrometry (LC-MS/MS) in negative electrospray ionization mode. No acid was used in the entire procedure. Single laboratory validation of the method was performed in terms of linearity, precision and accuracy. A low detection limit at ppb level was achieved. Satisfactory recoveries were obtained using spiked samples at three different concentration levels. This validated method could be used in the routine inspection of the advantame level in food. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=advantame" title="advantame">advantame</a>, <a href="https://publications.waset.org/abstracts/search?q=food" title=" food"> food</a>, <a href="https://publications.waset.org/abstracts/search?q=LC-MS%2FMS" title=" LC-MS/MS"> LC-MS/MS</a>, <a href="https://publications.waset.org/abstracts/search?q=sweetener" title=" sweetener"> sweetener</a> </p> <a href="https://publications.waset.org/abstracts/26887/determination-of-a-novel-artificial-sweetener-advantame-in-food-by-liquid-chromatography-tandem-mass-spectrometry" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/26887.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">475</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3649</span> Composition and in Vitro Antimicrobial Activity of Three Eryngium L. Species</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=R.%20Mickiene">R. Mickiene</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20Friese"> A. Friese</a>, <a href="https://publications.waset.org/abstracts/search?q=U.%20Rosler"> U. Rosler</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20Maruska"> A. Maruska</a>, <a href="https://publications.waset.org/abstracts/search?q=O.%20Ragazinskiene"> O. Ragazinskiene </a> </p> <p class="card-text"><strong>Abstract:</strong></p> This research focuses on phytochemistry and antimicrobial activities of compounds isolated and identified from three species of Eryngium. The antimicrobial activity of extracts from Eryngiumplanum L., Eryngium maritimum L., Eryngium campestre L. grown in Lithuania, were tested by the method of series dilutions, against different bacteria species: Escherichia coli, Proteus vulgaris and Staphylococcus aureus with and without antibiotic resistances, originating from livestock. The antimicrobial activity of extracts was described by determination of the minimal inhibitory concentration. Preliminary results show that the minimal inhibitory concentration range between 8.0 % and 17.0 % for the different Eryngium extracts and bacterial species.The total amounts ofphenolic compounds and total amounts of flavonoids were tested in the methanolic extracts of the plants. Identification and evaluation of the phenolic compounds were performed by liquid chromatography. The essential oils were analyzed by gas chromatography mass spectrometry. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=antimicrobial%20activities" title="antimicrobial activities">antimicrobial activities</a>, <a href="https://publications.waset.org/abstracts/search?q=Eryngium%20L.%20species" title=" Eryngium L. species"> Eryngium L. species</a>, <a href="https://publications.waset.org/abstracts/search?q=essential%20oils" title=" essential oils"> essential oils</a>, <a href="https://publications.waset.org/abstracts/search?q=gas%20chromatography%20mass%20spectrometry" title=" gas chromatography mass spectrometry"> gas chromatography mass spectrometry</a> </p> <a href="https://publications.waset.org/abstracts/4219/composition-and-in-vitro-antimicrobial-activity-of-three-eryngium-l-species" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/4219.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">446</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3648</span> Comparison of Different Methods of Microorganism's Identification from a Copper Mining in Pará, Brazil </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Louise%20H.%20Gracioso">Louise H. Gracioso</a>, <a href="https://publications.waset.org/abstracts/search?q=Marcela%20P.G.%20Baltazar"> Marcela P.G. Baltazar</a>, <a href="https://publications.waset.org/abstracts/search?q=Ingrid%20R.%20Avanzi"> Ingrid R. Avanzi</a>, <a href="https://publications.waset.org/abstracts/search?q=Bruno%20Karolski"> Bruno Karolski</a>, <a href="https://publications.waset.org/abstracts/search?q=Luciana%20J.%20Gimenes"> Luciana J. Gimenes</a>, <a href="https://publications.waset.org/abstracts/search?q=Claudio%20O.%20Nascimento"> Claudio O. Nascimento</a>, <a href="https://publications.waset.org/abstracts/search?q=Elen%20A.%20Perpetuo"> Elen A. Perpetuo </a> </p> <p class="card-text"><strong>Abstract:</strong></p> Introduction: Higher copper concentrations promote a selection pressure on organisms such as plants, fungi and bacteria, which allows surviving only the resistant organisms to the contaminated site. This selective pressure keeps only the organisms most resistant to a specific condition and subsequently increases their bioremediation potential. Despite the bacteria importance for biosphere maintenance, it is estimated that only a small fraction living microbial species has been described and characterized. Due to the molecular biology development, tools based on analysis 16S ribosomal RNA or another specific gene are making a new scenario for the characterization studies and identification of microorganisms in the environment. News identification of microorganisms methods have also emerged like Biotyper (MALDI / TOF), this method mass spectrometry is subject to the recognition of spectroscopic patterns of conserved and features proteins for different microbial species. In view of this, this study aimed to isolate bacteria resistant to copper present in a Copper Processing Area (Sossego Mine, Canaan, PA) and identifies them in two different methods: Recent (spectrometry mass) and conventional. This work aimed to use them for a future bioremediation of this Mining. Material and Methods: Samples were collected at fifteen different sites of five periods of times. Microorganisms were isolated from mining wastes by culture enrichment technique; this procedure was repeated 4 times. The isolates were inoculated into MJS medium containing different concentrations of chloride copper (1mM, 2.5mM, 5mM, 7.5mM and 10 mM) and incubated in plates for 72 h at 28 ºC. These isolates were subjected to mass spectrometry identification methods (Biotyper – MALDI/TOF) and 16S gene sequencing. Results: A total of 105 strains were isolated in this area, bacterial identification by mass spectrometry method (MALDI/TOF) achieved 74% agreement with the conventional identification method (16S), 31% have been unsuccessful in MALDI-TOF and 2% did not obtain identification sequence the 16S. These results show that Biotyper can be a very useful tool in the identification of bacteria isolated from environmental samples, since it has a better value for money (cheap and simple sample preparation and MALDI plates are reusable). Furthermore, this technique is more rentable because it saves time and has a high performance (the mass spectra are compared to the database and it takes less than 2 minutes per sample). <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=copper%20mining%20area" title="copper mining area">copper mining area</a>, <a href="https://publications.waset.org/abstracts/search?q=bioremediation" title=" bioremediation"> bioremediation</a>, <a href="https://publications.waset.org/abstracts/search?q=microorganisms" title=" microorganisms"> microorganisms</a>, <a href="https://publications.waset.org/abstracts/search?q=identification" title=" identification"> identification</a>, <a href="https://publications.waset.org/abstracts/search?q=MALDI%2FTOF" title=" MALDI/TOF"> MALDI/TOF</a>, <a href="https://publications.waset.org/abstracts/search?q=RNA%2016S" title=" RNA 16S"> RNA 16S</a> </p> <a href="https://publications.waset.org/abstracts/30563/comparison-of-different-methods-of-microorganisms-identification-from-a-copper-mining-in-para-brazil" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/30563.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">378</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3647</span> Synthesis, Characterization and Antibacterial Screening of 3-Hydroxy-2-[3-(2/3/4-Methoxybenzoyl)Thioureido]Butyric Acid</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=M.%20S.%20M.%20Yusof">M. S. M. Yusof</a>, <a href="https://publications.waset.org/abstracts/search?q=R.%20Ramli"> R. Ramli</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20K.%20C.%20Soh"> S. K. C. Soh</a>, <a href="https://publications.waset.org/abstracts/search?q=N.%20Ismail"> N. Ismail</a>, <a href="https://publications.waset.org/abstracts/search?q=N.%20Ngah"> N. Ngah</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This study presents the synthesis of a series of methoxybenzoylthiourea amino acid derivatives. The compounds were obtained from the reactions between 2/3/4-methoxybenzoyl isothiocyanate with threonine. All of the compounds were characterized via mass spectrometry, <sup>1</sup>H and <sup>13</sup>C NMR spectrometry, UV-Vis spectrophotometer and FT-IR spectroscopy. Mass spectra for all of the compounds showed the presence of molecular ion [M]<sup>+</sup> peaks at <em>m</em>/<em>z</em> 312, which are in agreement to the calculated molecular weight. For <sup>1</sup>H NMR spectra, the presence of OC<em>H</em><sub>3</sub>, C=S-N<em>H</em> and C=O-N<em>H</em> protons were observed within range of δ<sub>H </sub>3.8-4.0 ppm, 11.1-11.5 ppm and 10.0-11.5 ppm, respectively. <sup>13</sup>C NMR spectra in all compounds displayed the presence of O<em>C</em>H<sub>3</sub>, <em>C</em>=O-NH,<em> C</em>=O-OH and <em>C</em>=S carbon resonances within range of δ<sub>C </sub>55.0-57.0 ppm, 165.0-168.0 ppm, 170.0-171.0 ppm and 180.0-182.0 ppm, respectively. In UV spectra, two absorption bands have been observed and both were assigned to the n-π* and π-π* transitions. Six vibrational modes of <em>v</em>(N-H), <em>v</em>(O-H), <em>v</em>(C=O-OH), <em>v</em>(C=O-NH), <em>v</em>(C=C) aromatic and <em>v</em>(C=S) appeared in the FT-IR spectra within the range of 3241-3467 cm<sup>-1</sup>, 2976-3302 cm<sup>-1</sup>, 1720-1768 cm<sup>-1</sup>, 1655-1672 cm<sup>-1</sup>, 1519-1525 cm<sup>-1</sup> and 754-763 cm<sup>-1</sup>, respectively. The antibacterial activity for all of the compounds was screened against <em>Staphylococcus aureus</em>, <em>Staphylococcus epidermidis</em>, <em>Salmonella typhimurium</em> and <em>Escherichia coli</em>. However, no activity was observed. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=methoxybenzoyl%20isothiocyanate" title="methoxybenzoyl isothiocyanate">methoxybenzoyl isothiocyanate</a>, <a href="https://publications.waset.org/abstracts/search?q=amino%20acid" title=" amino acid"> amino acid</a>, <a href="https://publications.waset.org/abstracts/search?q=threonine" title=" threonine"> threonine</a>, <a href="https://publications.waset.org/abstracts/search?q=antibacterial" title=" antibacterial"> antibacterial</a> </p> <a href="https://publications.waset.org/abstracts/68578/synthesis-characterization-and-antibacterial-screening-of-3-hydroxy-2-3-234-methoxybenzoylthioureidobutyric-acid" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/68578.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">332</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3646</span> Assessing the Mass Concentration of Microplastics and Nanoplastics in Wastewater Treatment Plants by Pyrolysis Gas Chromatography−Mass Spectrometry</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Yanghui%20Xu">Yanghui Xu</a>, <a href="https://publications.waset.org/abstracts/search?q=Qin%20Ou"> Qin Ou</a>, <a href="https://publications.waset.org/abstracts/search?q=Xintu%20Wang"> Xintu Wang</a>, <a href="https://publications.waset.org/abstracts/search?q=Feng%20Hou"> Feng Hou</a>, <a href="https://publications.waset.org/abstracts/search?q=Peng%20Li"> Peng Li</a>, <a href="https://publications.waset.org/abstracts/search?q=Jan%20Peter%20van%20der%20Hoek"> Jan Peter van der Hoek</a>, <a href="https://publications.waset.org/abstracts/search?q=Gang%20Liu"> Gang Liu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The level and removal of microplastics (MPs) in wastewater treatment plants (WWTPs) has been well evaluated by the particle number, while the mass concentration of MPs and especially nanoplastics (NPs) remains unclear. In this study, microfiltration, ultrafiltration and hydrogen peroxide digestion were used to extract MPs and NPs with different size ranges (0.01−1, 1−50, and 50−1000 μm) across the whole treatment schemes in two WWTPs. By identifying specific pyrolysis products, pyrolysis gas chromatography−mass spectrometry were used to quantify their mass concentrations of selected six types of polymers (i.e., polymethyl methacrylate (PMMA), polypropylene (PP), polystyrene (PS), polyethylene (PE), polyethylene terephthalate (PET), and polyamide (PA)). The mass concentrations of total MPs and NPs decreased from 26.23 and 11.28 μg/L in the influent to 1.75 and 0.71 μg/L in the effluent, with removal rates of 93.3 and 93.7% in plants A and B, respectively. Among them, PP, PET and PE were the dominant polymer types in wastewater, while PMMA, PS and PA only accounted for a small part. The mass concentrations of NPs (0.01−1 μm) were much lower than those of MPs (>1 μm), accounting for 12.0−17.9 and 5.6− 19.5% of the total MPs and NPs, respectively. Notably, the removal efficiency differed with the polymer type and size range. The low-density MPs (e.g., PP and PE) had lower removal efficiency than high-density PET in both plants. Since particles with smaller size could pass the tertiary sand filter or membrane filter more easily, the removal efficiency of NPs was lower than that of MPs with larger particle size. Based on annual wastewater effluent discharge, it is estimated that about 0.321 and 0.052 tons of MPs and NPs were released into the river each year. Overall, this study investigated the mass concentration of MPs and NPs with a wide size range of 0.01−1000 μm in wastewater, which provided valuable information regarding the pollution level and distribution characteristics of MPs, especially NPs, in WWTPs. However, there are limitations and uncertainties in the current study, especially regarding the sample collection and MP/NP detection. The used plastic items (e.g., sampling buckets, ultrafiltration membranes, centrifugal tubes, and pipette tips) may introduce potential contamination. Additionally, the proposed method caused loss of MPs, especially NPs, which can lead to underestimation of MPs/NPs. Further studies are recommended to address these challenges about MPs/NPs in wastewater. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=microplastics" title="microplastics">microplastics</a>, <a href="https://publications.waset.org/abstracts/search?q=nanoplastics" title=" nanoplastics"> nanoplastics</a>, <a href="https://publications.waset.org/abstracts/search?q=mass%20concentration" title=" mass concentration"> mass concentration</a>, <a href="https://publications.waset.org/abstracts/search?q=WWTPs" title=" WWTPs"> WWTPs</a>, <a href="https://publications.waset.org/abstracts/search?q=Py-GC%2FMS" title=" Py-GC/MS"> Py-GC/MS</a> </p> <a href="https://publications.waset.org/abstracts/165249/assessing-the-mass-concentration-of-microplastics-and-nanoplastics-in-wastewater-treatment-plants-by-pyrolysis-gas-chromatographymass-spectrometry" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/165249.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">281</span> </span> </div> </div> <ul class="pagination"> <li class="page-item disabled"><span class="page-link">&lsaquo;</span></li> <li class="page-item active"><span class="page-link">1</span></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=mass%20spectrometry&page=2">2</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=mass%20spectrometry&page=3">3</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=mass%20spectrometry&page=4">4</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=mass%20spectrometry&page=5">5</a></li> <li class="page-item"><a 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