PERFORMA GENOTIP IKAN TAMBAKAN Helostoma temminckii (CUVIER, 1829) POPULASI SUMATERA, JAWA DAN KALIMANTAN DENGAN METODE RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD)

<!DOCTYPE html> <html lang="en-US" xml:lang="en-US"> <head> <meta charset="utf-8"> <meta name="viewport" content="width=device-width, initial-scale=1.0"> <title> PERFORMA GENOTIP IKAN TAMBAKAN Helostoma temminckii (CUVIER, 1829) POPULASI SUMATERA, JAWA DAN KALIMANTAN DENGAN METODE RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) | JURNAL SAINS NATURAL </title> <link rel="icon" href="https://ejournalunb.ac.id/public/journals/2/favicon_en_US.png"> <meta name="generator" content="Open Journal Systems 3.3.0.12"> <meta name="google-site-verification" content="FnXpFP0CxoCW3kpkZuA-kC-RKV1DhgYUNPieBQ1EyOA" /> <link rel="schema.DC" href="http://purl.org/dc/elements/1.1/" /> <meta name="DC.Creator.PersonalName" content="M. H. Fariduddin Ath-thar"/> <meta name="DC.Creator.PersonalName" content="Intan Putriana"/> <meta name="DC.Creator.PersonalName" content="Dinar Tri Soelistyowati"/> <meta name="DC.Creator.PersonalName" content="Rudhy Gustiano"/> <meta name="DC.Date.created" scheme="ISO8601" content="2017-12-01"/> <meta name="DC.Date.dateSubmitted" scheme="ISO8601" content="2017-12-01"/> <meta name="DC.Date.issued" scheme="ISO8601" content="2014-01-30"/> <meta name="DC.Date.modified" scheme="ISO8601" content="2023-11-29"/> <meta name="DC.Description" xml:lang="en" content="Genotype performance of Tambakan, Helostoma temminckii (Cuvier, 1829) from Sumatera, Java and Kalimantan Polulation using Random Amplified Polymorphic DNA (RAPD)Â Â Â Â Â Â Â Tambakan, kissing gourami (Helostoma temminckii) is well known as a freshwater tropical Â species from Southeast Asia. In Indonesia, tambakan is an important commodity. However, total production of tambakan tends to decrease. Therefore, domestication has urgently to be done to solve the problem of Tambakan population. Three different tambakan populations from Sumatera, Java and Kalimantan were observed to find good genetic resources for culture activity. This study aimed to investigate the genetic variation of tambakan especially from West Java, Jambi, and South Kalimantan province using RAPD. The result showed that the highest polymorphism and heterozygosity was from South Kalimantan population among others. The three population observed had the fragment size ranged from 100- 2000 bp. The highest genetic distance was between Sumatera and Kalimantan (0,2877), while the lowest was between Kalimantan and Java (0,1961). Key words: Helostoma temminckii, genetic, heterozigosity, genetic relationshipÂ ABSTRAKÂ Â Â Â Â Â Â Ikan tambakan (Helostoma temminckii) adalah salah satu jenis ikan air tawar yang berasal dari wilayah tropis, tepatnya Asia Tenggara. Di Indonesia ikan tambakan merupakan salah satu ikan komoditas penting. Saat ini, jumlah produksi ikan tambakan cenderung menurun. Sehingga domestikasi sangat diperlukan untuk mengatasi masalah peneurunan populasi tambakan Tiga populasi ikan tambakan dari Sumatera, Jawa dan Kalimantan diobeservasi untuk mendapatkan sumber genetic terbaik yang dapat digunakan untuk kegiatan budidaya.. Penelitian ini bertujuan untuk mengidentifikasi keragaman genetika populasi ikan tambakan Jambi, Jawa Barat dan Kalimantan Selatan menggunakan metode RAPD. Hasil menunjukkan bahwa polimorfisme dan heterosigositas tertinggi terdapat pada populasi ikan tambakan Kalimantan Selatan jika dibandingkan dengan populasi ikan tambakan lainnya. Ukuran fragmen DNA teramplifikasi berkisar antara 100-2000 bp. Jarak genetik paling jauh adalah antara populasi tambakan Sumatera dengan Kalimantan (0,2877), sedangkan jarak genetik terendah adalah tambakan Kalimantan dengan Jawa (0,1961).Kata kunci: Helostoma temminckii, genetik, heterosigositas, kekerabatan"/> <meta name="DC.Format" scheme="IMT" content="application/pdf"/> <meta name="DC.Identifier" content="77"/> <meta name="DC.Identifier.pageNumber" content="68-75"/> <meta name="DC.Identifier.DOI" content="10.31938/jsn.v4i1.77"/> <meta name="DC.Identifier.URI" content="https://ejournalunb.ac.id/index.php/JSN/article/view/77"/> <meta name="DC.Language" scheme="ISO639-1" content="en"/> <meta name="DC.Rights" content="Copyright (c) 2017 M. H. Fariduddin Ath-thar, Intan Putriana, Dinar Tri Soelistyowati, Rudhy Gustiano"/> <meta name="DC.Rights" content="https://creativecommons.org/licenses/by-sa/4.0"/> <meta name="DC.Source" content="JURNAL SAINS NATURAL"/> <meta name="DC.Source.ISSN" content="2621-508X"/> <meta name="DC.Source.Issue" content="1"/> <meta name="DC.Source.Volume" content="4"/> <meta name="DC.Source.URI" content="https://ejournalunb.ac.id/index.php/JSN"/> <meta name="DC.Title" content="PERFORMA GENOTIP IKAN TAMBAKAN Helostoma temminckii (CUVIER, 1829) POPULASI SUMATERA, JAWA DAN KALIMANTAN DENGAN METODE RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD)"/> <meta name="DC.Type" content="Text.Serial.Journal"/> <meta name="DC.Type.articleType" content="Research Articles"/> <meta name="gs_meta_revision" content="1.1"/> <meta name="citation_journal_title" content="JURNAL SAINS NATURAL"/> <meta name="citation_journal_abbrev" content="J. Sains Nat."/> <meta name="citation_issn" content="2621-508X"/> <meta name="citation_author" content="M. H. Fariduddin Ath-thar"/> <meta name="citation_author_institution" content="Balai Penelitian dan Pengembangan Budidaya Air Tawar Bogor"/> <meta name="citation_author" content="Intan Putriana"/> <meta name="citation_author_institution" content="Departemen Budidaya Periaran, Institut Pertanian Bogor"/> <meta name="citation_author" content="Dinar Tri Soelistyowati"/> <meta name="citation_author_institution" content="Departemen Budidaya Periaran, Institut Pertanian Bogor"/> <meta name="citation_author" content="Rudhy Gustiano"/> <meta name="citation_author_institution" content="Balai Penelitian dan Pengembangan Budidaya Air Tawar Bogor"/> <meta name="citation_title" content="PERFORMA GENOTIP IKAN TAMBAKAN Helostoma temminckii (CUVIER, 1829) POPULASI SUMATERA, JAWA DAN KALIMANTAN DENGAN METODE RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD)"/> <meta name="citation_language" content="en"/> <meta name="citation_date" content="2014"/> <meta name="citation_volume" content="4"/> <meta name="citation_issue" content="1"/> <meta name="citation_firstpage" content="68"/> <meta name="citation_lastpage" content="75"/> <meta name="citation_doi" content="10.31938/jsn.v4i1.77"/> <meta name="citation_abstract_html_url" content="https://ejournalunb.ac.id/index.php/JSN/article/view/77"/> <meta name="citation_pdf_url" content="https://ejournalunb.ac.id/index.php/JSN/article/download/77/75"/> <meta name="citation_reference" content="Ali, B.A., Huang T.H, Qin D. N., Wang X.M. 2004. A review of random amplified polymorphic DNA (RAPD) markers in fish research. Reviews in Fish Biology and Fisheries 14: 443-453"/> <meta name="citation_reference" content="Dieffenbach, C.W dan Dveksler G. S. 2003. PCR primer: a laboratory manual. Cold Spring Harbor Laboratory Press. 520 pp"/> <meta name="citation_reference" content="Direktorat Jendral Perikanan Tangkap. 2012. Statistik perikanan tangkap Indonesia 2012. Kementerian Kelautan Per-ikanan"/> <meta name="citation_reference" content="Dunham, R.A. 20011. Aquaculture and fisheries biotechnology: Genetic Approach. CABI Publishing, Cambridge, USA. P: 85-99."/> <meta name="citation_reference" content="FAO. 2010. Helostoma temminckii (Cuvier, 1829). Species fact sheet. FAO, Roma"/> <meta name="citation_reference" content="Falconer, F.S. dan Mackay T.F.C. 1996. Introduction to quantitative genetics. Longman England. 464pp"/> <meta name="citation_reference" content="Gjedrem, T. 2005. Selection and breeding programs in aquaculture. Springer. New York. P: 251-277"/> <meta name="citation_reference" content="Gustiano R., Kusmini I.I., Ath-thar M.H.F. Mengenal sumber daya genetik ikan spesifik lokal air tawar indonesia untuk pengembangan budidaya. PT Penerbit IPB Press."/> <meta name="citation_reference" content="Kapuscinski, A.R dan Jacobson L.D. 2007. Genetic guideline for fisheries management. University of Minnesota, Minnesota Sea Grant Collge Program. Sea Grant Research Report 17, 1-66"/> <meta name="citation_reference" content="Kirpichnikov, V.S. Genetic Bases of Fish Selection. Springer Verlag Berlin. 410pp"/> <meta name="citation_reference" content="Nei, M and Tajima F.. 1981. DNA Polymorphism detectable by resctriction endonuclease. Genetics 97: 146-163."/> <meta name="citation_reference" content="Nugroho, E., M. Takagi, N. Taniguchi. 1997. Practical manual on detection of DNA polymorphism in fish population study. Bulletin of Marine Sciences and Fisheries 17:109-129."/> <meta name="citation_reference" content="Rainboth, W.L. 1996. FAO species identification field guide for fishery purposes. Fishes of the Cambodian Mekong. Rome, FAO. 265 pp."/> <meta name="citation_reference" content="Tave, D. 1993. Genetics for fish hatchery managers. Kluwer Academic Publications. Netherland. 415pp"/> <link rel="stylesheet" href="https://ejournalunb.ac.id/index.php/JSN/$$$call$$$/page/page/css?name=stylesheet" type="text/css" /><link rel="stylesheet" href="https://ejournalunb.ac.id/lib/pkp/styles/fontawesome/fontawesome.css?v=3.3.0.12" type="text/css" /><link rel="stylesheet" href="https://ejournalunb.ac.id/plugins/themes/material/vendor/fontawesome/css/all.min.css?v=3.3.0.12" type="text/css" /><link rel="stylesheet" href="https://ejournalunb.ac.id/plugins/themes/material/vendor/mdb/css/mdb.min.css?v=3.3.0.12" type="text/css" /><link rel="stylesheet" href="https://ejournalunb.ac.id/plugins/themes/material/vendor/aos/aos.css?v=3.3.0.12" type="text/css" /><link rel="stylesheet" href="https://ejournalunb.ac.id/plugins/themes/material/vendor/swiper/swiper-bundle.min.css?v=3.3.0.12" type="text/css" /><style type="text/css">.pkp_structure_head { background: center / cover no-repeat url("https://ejournalunb.ac.id/public/journals/2/homepageImage_en_US.jpg");}</style><link rel="stylesheet" href="https://ejournalunb.ac.id/plugins/generic/citations/css/citations.css?v=3.3.0.12" type="text/css" /><link rel="stylesheet" href="https://ejournalunb.ac.id/public/site/styleSheet.css?v=3.3.0.12" type="text/css" /> </head> <body class="pkp_page_article pkp_op_view has_site_logo" dir="ltr">  <header id="header" class="fixed-top d-flex align-items-center"> <div class="container d-flex justify-content-between align-items-center"> <div class="logo"> <a href="https://ejournalunb.ac.id/index.php/JSN/index" class="navbar-brand _is_img"> <img src="https://ejournalunb.ac.id/public/journals/2/pageHeaderLogoImage_en_US.png" width="2953" height="548" class="img-fluid" style="max-width: 180px;"/> </a> </div> <nav id="navbar" class="navbar navbar-dark flex-row shadow-0"> <ul id="_navigationPrimary" class="_pkp_navigation_primary navbar-nav ml-auto"> <li class=" nav-item"> <a href="https://ejournalunb.ac.id/index.php/JSN/index" class="nav-link"> HOME </a> </li> <li class=" nav-item"> <div class="dropdown"> <a href="https://ejournalunb.ac.id/index.php/JSN/about" class="dropdown-toggle nav-link" type="button" id="_navigationPrimary" data-mdb-toggle="dropdown" aria-expanded="false" > ABOUT </a> <ul class="dropdown-menu dropdown-menu-xxl-end dropdown-menu-light" aria-labelledby="_navigationPrimary"> <li class=""> <a href="https://ejournalunb.ac.id/index.php/JSN/about" class="dropdown-item"> ABOUT THE JOURNAL </a> </li> <li class=""> <a href="https://ejournalunb.ac.id/index.php/JSN/history" class="dropdown-item"> JOURNAL HISTORY </a> </li> <li class=""> <a href="https://ejournalunb.ac.id/index.php/JSN/about/contact" class="dropdown-item"> CONTACT </a> </li> </ul> </div> </li> <li class=" nav-item"> <div class="dropdown"> <a href="https://ejournalunb.ac.id/index.php/JSN/editorial_policy" class="dropdown-toggle nav-link" type="button" id="_navigationPrimary" data-mdb-toggle="dropdown" aria-expanded="false" > POLICY </a> <ul class="dropdown-menu dropdown-menu-xxl-end dropdown-menu-light" aria-labelledby="_navigationPrimary"> <li class=""> <a href="https://ejournalunb.ac.id/index.php/JSN/focusscope" class="dropdown-item"> FOCUS AND SCOPE </a> </li> <li class=""> <a href="https://ejournalunb.ac.id/index.php/JSN/peer-review" class="dropdown-item"> PEER REVIEW PROCESS </a> </li> <li class=""> <a href="https://ejournalunb.ac.id/index.php/JSN/publication-frequency" class="dropdown-item"> PUBLICATION FREQUENCY </a> </li> <li class=""> <a href="https://ejournalunb.ac.id/index.php/JSN/open-access-policy" class="dropdown-item"> OPEN ACCCESS POLICY </a> </li> <li class=""> <a href="https://ejournalunb.ac.id/index.php/JSN/indexing" class="dropdown-item"> INDEXING </a> </li> <li class=""> <a href="https://ejournalunb.ac.id/index.php/JSN/archiving" class="dropdown-item"> ARCHIVING </a> </li> <li class=""> <a href="https://ejournalunb.ac.id/index.php/JSN/screening-plagiarism" class="dropdown-item"> SCREENING PLAGIARISM </a> </li> <li class=""> <a href="https://ejournalunb.ac.id/index.php/JSN/retraction" class="dropdown-item"> RETRACTION </a> </li> </ul> </div> </li> <li class=" nav-item"> <div class="dropdown"> <a href="https://ejournalunb.ac.id/index.php/JSN/about" class="dropdown-toggle nav-link" type="button" id="_navigationPrimary" data-mdb-toggle="dropdown" aria-expanded="false" > PEOPLE </a> <ul class="dropdown-menu dropdown-menu-xxl-end dropdown-menu-light" aria-labelledby="_navigationPrimary"> <li class=""> <a href="https://ejournalunb.ac.id/index.php/JSN/about/editorialTeam" class="dropdown-item"> EDITORIAL TEAM </a> </li> <li class=""> <a href="https://ejournalunb.ac.id/index.php/JSN/peer" class="dropdown-item"> PEER REVIEWERS </a> </li> </ul> </div> </li> <li class=" nav-item"> <div class="dropdown"> <a href="https://ejournalunb.ac.id/index.php/JSN/about" class="dropdown-toggle nav-link" type="button" id="_navigationPrimary" data-mdb-toggle="dropdown" aria-expanded="false" > FOR AUTHOR </a> <ul class="dropdown-menu dropdown-menu-xxl-end dropdown-menu-light" aria-labelledby="_navigationPrimary"> <li class=""> <a href="https://ejournalunb.ac.id/index.php/JSN/guide" class="dropdown-item"> AUTHOR GUIDLINES </a> </li> <li class=""> <a href="https://ejournalunb.ac.id/index.php/JSN/APC" class="dropdown-item"> ARTICLE PROCESSING CHARGE </a> </li> <li class=""> <a href="https://ejournalunb.ac.id/index.php/JSN/onlinesubmission" class="dropdown-item"> ONLINE SUBMISSION </a> </li> <li class=""> <a href="https://ejournalunb.ac.id/index.php/JSN/publication-ethics" class="dropdown-item"> PUBLICATION ETHICS </a> </li> <li class=""> <a href="https://ejournalunb.ac.id/index.php/JSN/copyright-notice" class="dropdown-item"> COPYRIGHT NOTICE </a> </li> <li class=""> <a href="https://ejournalunb.ac.id/index.php/JSN/about/privacy" class="dropdown-item"> PRIVACY STATEMENT </a> </li> </ul> </div> </li> <li class=" nav-item"> <div class="dropdown"> <a href="https://ejournalunb.ac.id/index.php/JSN/about" class="dropdown-toggle nav-link" type="button" id="_navigationPrimary" data-mdb-toggle="dropdown" aria-expanded="false" > ISSUES </a> <ul class="dropdown-menu dropdown-menu-xxl-end dropdown-menu-light" aria-labelledby="_navigationPrimary"> <li class=""> <a href="https://ejournalunb.ac.id/index.php/JSN/issue/current" class="dropdown-item"> CURRENT </a> </li> <li class=""> <a href="https://ejournalunb.ac.id/index.php/JSN/issue/archive" class="dropdown-item"> ARCHIVES </a> </li> </ul> </div> </li> </ul> </nav> <nav class="navbar navbar-expand-lg navbar-dark scrolling-navbar d-flex flex-column shadow-0"> <div class="container"> <div class="navbar-collapse d-flex justify-content-end"> <ul class="navbar-nav mr-auto flex-row "> <li class="nav-item "> <div class="dropdown"> <a href="#" class="nav-link" type="button" id="dropdownSidebar" data-mdb-toggle="dropdown" aria-expanded="false"> <i class="fas fa-globe"></i> </a> <ul class="dropdown-menu dropdown-menu-xxl-end dropdown-menu-light" aria-labelledby="dropdownSidebar"> <li class="locale_en_US current" lang="en-US"> <a href="https://ejournalunb.ac.id/index.php/JSN/user/setLocale/en_US?source=%2Findex.php%2FJSN%2Farticle%2Fview%2F77" class="dropdown-item"> English </a> </li> </ul> </div> </li> <li class=" nav-item"> <a href="https://ejournalunb.ac.id/index.php/JSN/search" class="nav-link mx-1"> <i class="fas fa-search"></i> </a> </li> </ul> <ul id="_navigationUser" class="_pkp_navigation_user flex-row justify-content-end navbar-nav ml-auto"> <li class="profile px-2 px-md-0 nav-item"> <a href="https://ejournalunb.ac.id/" class="nav-link"> ejournalunb </a> </li> <li class="profile px-2 px-md-0 nav-item"> <a href="https://ejournalunb.ac.id/index.php/JSN/user/register" class="nav-link"> Register </a> </li> <li class="profile px-2 px-md-0 nav-item"> <a href="https://ejournalunb.ac.id/index.php/JSN/login" class="nav-link"> Login </a> </li> </ul> </div> </div> </nav> <button type="button" class="navbar-toggler text-white d-lg-none" data-mdb-toggle="modal" data-mdb-target="#exampleModal"> <i class="fas fa-bars"></i> </button>  <div class="modal fade" id="exampleModal" tabindex="-1" aria-labelledby="exampleModalLabel" aria-hidden="true"> <div class="modal-dialog"> <div class="modal-content"> <div class="modal-header"> <h5 class="modal-title" id="exampleModalLabel"></h5> <button type="button" class="btn-close" data-mdb-dismiss="modal" aria-label="Close"></button> </div> <div class="modal-body"> <div> <ul id="_navigationPrimary" class="_pkp_navigation_primary d-block navbar-nav ml-auto"> <li class="text-dark nav-item"> <a href="https://ejournalunb.ac.id/index.php/JSN/index" class="nav-link"> HOME </a> </li> <li class="text-dark nav-item"> <div class="dropdown"> <a href="https://ejournalunb.ac.id/index.php/JSN/about" class="dropdown-toggle nav-link" type="button" id="_navigationPrimary" data-mdb-toggle="dropdown" aria-expanded="false" > ABOUT </a> <ul class="dropdown-menu dropdown-menu-xxl-end dropdown-menu-light" aria-labelledby="_navigationPrimary"> <li class="text-dark"> <a href="https://ejournalunb.ac.id/index.php/JSN/about" class="dropdown-item"> ABOUT THE JOURNAL </a> </li> <li class="text-dark"> <a href="https://ejournalunb.ac.id/index.php/JSN/history" class="dropdown-item"> JOURNAL HISTORY </a> </li> <li class="text-dark"> <a href="https://ejournalunb.ac.id/index.php/JSN/about/contact" class="dropdown-item"> CONTACT </a> </li> </ul> </div> </li> <li class="text-dark nav-item"> <div class="dropdown"> <a href="https://ejournalunb.ac.id/index.php/JSN/editorial_policy" class="dropdown-toggle nav-link" type="button" id="_navigationPrimary" data-mdb-toggle="dropdown" aria-expanded="false" > POLICY </a> <ul class="dropdown-menu dropdown-menu-xxl-end dropdown-menu-light" aria-labelledby="_navigationPrimary"> <li class="text-dark"> <a href="https://ejournalunb.ac.id/index.php/JSN/focusscope" class="dropdown-item"> FOCUS AND SCOPE </a> </li> <li class="text-dark"> <a href="https://ejournalunb.ac.id/index.php/JSN/peer-review" class="dropdown-item"> PEER REVIEW PROCESS </a> </li> <li class="text-dark"> <a href="https://ejournalunb.ac.id/index.php/JSN/publication-frequency" class="dropdown-item"> PUBLICATION FREQUENCY </a> </li> <li class="text-dark"> <a href="https://ejournalunb.ac.id/index.php/JSN/open-access-policy" class="dropdown-item"> OPEN ACCCESS POLICY </a> </li> <li class="text-dark"> <a href="https://ejournalunb.ac.id/index.php/JSN/indexing" class="dropdown-item"> INDEXING </a> </li> <li class="text-dark"> <a href="https://ejournalunb.ac.id/index.php/JSN/archiving" class="dropdown-item"> ARCHIVING </a> </li> <li class="text-dark"> <a href="https://ejournalunb.ac.id/index.php/JSN/screening-plagiarism" class="dropdown-item"> SCREENING PLAGIARISM </a> </li> <li class="text-dark"> <a href="https://ejournalunb.ac.id/index.php/JSN/retraction" class="dropdown-item"> RETRACTION </a> </li> </ul> </div> </li> <li class="text-dark nav-item"> <div class="dropdown"> <a href="https://ejournalunb.ac.id/index.php/JSN/about" class="dropdown-toggle nav-link" type="button" id="_navigationPrimary" data-mdb-toggle="dropdown" aria-expanded="false" > PEOPLE </a> <ul class="dropdown-menu dropdown-menu-xxl-end dropdown-menu-light" aria-labelledby="_navigationPrimary"> <li class="text-dark"> <a href="https://ejournalunb.ac.id/index.php/JSN/about/editorialTeam" class="dropdown-item"> EDITORIAL TEAM </a> </li> <li class="text-dark"> <a href="https://ejournalunb.ac.id/index.php/JSN/peer" class="dropdown-item"> PEER REVIEWERS </a> </li> </ul> </div> </li> <li class="text-dark nav-item"> <div class="dropdown"> <a href="https://ejournalunb.ac.id/index.php/JSN/about" class="dropdown-toggle nav-link" type="button" id="_navigationPrimary" data-mdb-toggle="dropdown" aria-expanded="false" > FOR AUTHOR </a> <ul class="dropdown-menu dropdown-menu-xxl-end dropdown-menu-light" aria-labelledby="_navigationPrimary"> <li class="text-dark"> <a href="https://ejournalunb.ac.id/index.php/JSN/guide" class="dropdown-item"> AUTHOR GUIDLINES </a> </li> <li class="text-dark"> <a href="https://ejournalunb.ac.id/index.php/JSN/APC" class="dropdown-item"> ARTICLE PROCESSING CHARGE </a> </li> <li class="text-dark"> <a href="https://ejournalunb.ac.id/index.php/JSN/onlinesubmission" class="dropdown-item"> ONLINE SUBMISSION </a> </li> <li class="text-dark"> <a href="https://ejournalunb.ac.id/index.php/JSN/publication-ethics" class="dropdown-item"> PUBLICATION ETHICS </a> </li> <li class="text-dark"> <a href="https://ejournalunb.ac.id/index.php/JSN/copyright-notice" class="dropdown-item"> COPYRIGHT NOTICE </a> </li> <li class="text-dark"> <a href="https://ejournalunb.ac.id/index.php/JSN/about/privacy" class="dropdown-item"> PRIVACY STATEMENT </a> </li> </ul> </div> </li> <li class="text-dark nav-item"> <div class="dropdown"> <a href="https://ejournalunb.ac.id/index.php/JSN/about" class="dropdown-toggle nav-link" type="button" id="_navigationPrimary" data-mdb-toggle="dropdown" aria-expanded="false" > ISSUES </a> <ul class="dropdown-menu dropdown-menu-xxl-end dropdown-menu-light" aria-labelledby="_navigationPrimary"> <li class="text-dark"> <a href="https://ejournalunb.ac.id/index.php/JSN/issue/current" class="dropdown-item"> CURRENT </a> </li> <li class="text-dark"> <a href="https://ejournalunb.ac.id/index.php/JSN/issue/archive" class="dropdown-item"> ARCHIVES </a> </li> </ul> </div> </li> </ul> </div> <hr/> <div> <ul class="navbar-nav mr-auto flex-row d-block"> <li class="nav-item mx-0"> <div class="dropdown"> <a href="#" class="nav-link dropdown-toggle" type="button" id="dropdownSidebar" data-mdb-toggle="dropdown" aria-expanded="false"> Language </a> <ul class="dropdown-menu dropdown-menu-xxl-end dropdown-menu-light" aria-labelledby="dropdownSidebar"> <li class="locale_en_US current" lang="en-US"> <a href="https://ejournalunb.ac.id/index.php/JSN/user/setLocale/en_US?source=%2Findex.php%2FJSN%2Farticle%2Fview%2F77" class="dropdown-item"> English </a> </li> </ul> </div> </li> <li class="mx-0 nav-item"> <a href="https://ejournalunb.ac.id/index.php/JSN/search" class="nav-link"> Search </a> </li> </ul> <ul id="_navigationUser" class="_pkp_navigation_user d-block navbar-nav ml-auto"> <li class="profile text-dark nav-item"> <a href="https://ejournalunb.ac.id/" class="nav-link"> ejournalunb </a> </li> <li class="profile text-dark nav-item"> <a href="https://ejournalunb.ac.id/index.php/JSN/user/register" class="nav-link"> Register </a> </li> <li class="profile text-dark nav-item"> <a href="https://ejournalunb.ac.id/index.php/JSN/login" class="nav-link"> Login </a> </li> </ul> </div> </div> <div class="modal-footer"> </div> </div> </div> </div> </div> </header> <main class="" role="main"> <a id="pkp_content_main"></a> <section class="hero-section inner-page"> <div class="wave"> <svg width="1920px" height="265px" viewBox="0 0 1920 265" version="1.1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"> <g id="Page-1" stroke="none" stroke-width="1" fill="none" fill-rule="evenodd"> <g id="Apple-TV" transform="translate(0.000000, -402.000000)" fill="#FFFFFF"> <path d="M0,439.134243 C175.04074,464.89273 327.944386,477.771974 458.710937,477.771974 C654.860765,477.771974 870.645295,442.632362 1205.9828,410.192501 C1429.54114,388.565926 1667.54687,411.092417 1920,477.771974 L1920,667 L1017.15166,667 L0,667 L0,439.134243 Z" id="Path"></path> </g> </g> </svg> </div> <div class="container"> <div class="row align-items-center"> <div class="col-12"> <div class="row justify-content-center"> <div class="col-md-10 text-center hero-text"> <h1 data-aos="fade-up" data-aos-delay="">PERFORMA GENOTIP IKAN TAMBAKAN Helostoma temminckii (CUVIER, 1829) POPULASI SUMATERA, JAWA DAN KALIMANTAN DENGAN METODE RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD)</h1> <p class="mb-5" data-aos="fade-up" data-aos-delay="100"></p> </div> </div> </div> </div> </div> </section> <div class="page page_article"> <nav class="cmp_breadcrumbs" role="navigation" aria-label="You are here:"> <ol> <li> <a href="https://ejournalunb.ac.id/index.php/JSN/index"> Home </a> <span class="separator">/</span> </li> <li> <a href="https://ejournalunb.ac.id/index.php/JSN/issue/archive"> Archives </a> <span class="separator">/</span> </li> <li> <a href="https://ejournalunb.ac.id/index.php/JSN/issue/view/11"> Vol. 4 No. 1 (2014): Sains Natural </a> <span class="separator">/</span> </li> <li class="current" aria-current="page"> <span aria-current="page"> Research Articles </span> </li> </ol> </nav> <article class="obj_article_details"> <div class="d-md-flex"> <div class="main_entry"> <section class="item"> <h3 class="page_title"> PERFORMA GENOTIP IKAN TAMBAKAN Helostoma temminckii (CUVIER, 1829) POPULASI SUMATERA, JAWA DAN KALIMANTAN DENGAN METODE RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) </h3> </section> <section class="item authors"> <h2 class="pkp_screen_reader">Authors</h2> <ul class="authors"> <li> <span class="name"> M. H. Fariduddin Ath-thar </span> <span class="affiliation"> Balai Penelitian dan Pengembangan Budidaya Air Tawar Bogor </span> </li> <li> <span class="name"> Intan Putriana </span> <span class="affiliation"> Departemen Budidaya Periaran, Institut Pertanian Bogor </span> </li> <li> <span class="name"> Dinar Tri Soelistyowati </span> <span class="affiliation"> Departemen Budidaya Periaran, Institut Pertanian Bogor </span> </li> <li> <span class="name"> Rudhy Gustiano </span> <span class="affiliation"> Balai Penelitian dan Pengembangan Budidaya Air Tawar Bogor </span> </li> </ul> </section> <section class="item doi"> <h2 class="label"> DOI: </h2> <span class="value"> <a href="https://doi.org/10.31938/jsn.v4i1.77"> https://doi.org/10.31938/jsn.v4i1.77 </a> </span> </section> <section class="item abstract"> <h4 class="_label">Abstract</h4> <p><strong><em>Genotype performance of Tambakan, Helostoma temminckii (Cuvier, 1829) from Sumatera, Java and Kalimantan Polulation using Random Amplified Polymorphic DNA (RAPD)</em></strong></p><p><em>Â Â Â Â Â Â Â Tambakan, kissing gourami (Helostoma temminckii) is well known as a freshwater</em><em> tropical </em><em>Â species from Southeast Asia. In Indonesia, tambakan is an important commodity. However, total production of tambakan tend</em><em>s</em><em> to decrease. Therefore, domestication has urgently to be done to solve the problem of Tambakan population. Three different tambakan populations from Sumatera, Java and Kalimantan were observed to find good genetic resources for culture activity. This study aimed to investigate the genetic variation of tambakan </em><em>especially </em><em>from West Java, Jambi, and South Kalimantan province using RAPD. The result showed that the highest polymorphism and heterozygosity was from South Kalimantan population among others. The three population observed had the fragment size ranged from 100- 2000 bp. The highest genetic distance was between Sumatera and Kalimantan (0,2877), while the lowest was between Kalimantan and Java (0,1961). </em></p><p><em>Key words: Helostoma temminckii, genetic, heterozigosity, genetic relationship</em></p><p><strong>Â </strong></p><p><strong>ABSTRAK</strong></p><p>Â Â Â Â Â Â Â Ikan tambakan <em>(Helostoma temminckii) </em>adalah salah satu jenis ikan air tawar yang berasal dari wilayah tropis, tepatnya Asia Tenggara. Di Indonesia ikan tambakan merupakan salah satu ikan komoditas penting. Saat ini, jumlah produksi ikan tambakan cenderung menurun. Sehingga domestikasi sangat diperlukan untuk mengatasi masalah peneurunan populasi tambakan Tiga populasi ikan tambakan dari Sumatera, Jawa dan Kalimantan diobeservasi untuk mendapatkan sumber genetic terbaik yang dapat digunakan untuk kegiatan budidaya.. Penelitian ini bertujuan untuk mengidentifikasi keragaman genetika populasi ikan tambakan Jambi, Jawa Barat dan Kalimantan Selatan menggunakan metode RAPD. Hasil menunjukkan bahwa polimorfisme dan heterosigositas tertinggi terdapat pada populasi ikan tambakan Kalimantan Selatan jika dibandingkan dengan populasi ikan tambakan lainnya. Ukuran fragmen DNA teramplifikasi berkisar antara 100-2000 bp. Jarak genetik paling jauh adalah antara populasi tambakan Sumatera dengan Kalimantan (0,2877), sedangkan jarak genetik terendah adalah tambakan Kalimantan dengan Jawa (0,1961).</p>Kata kunci: <em>Helostoma temminckii</em>, genetik, heterosigositas, kekerabatan </section> <div class="item downloads_chart"> <h3 class="label"> Downloads </h3> <div class="value"> <canvas class="usageStatsGraph" data-object-type="Submission" data-object-id="77"></canvas> <div class="usageStatsUnavailable" data-object-type="Submission" data-object-id="77"> Download data is not yet available. </div> </div> </div> <section class="item references"> <h2 class="label"> References </h2> <div class="value"> <p>Ali, B.A., Huang T.H, Qin D. N., Wang X.M. 2004. A review of random amplified polymorphic DNA (RAPD) markers in fish research. Reviews in Fish Biology and Fisheries 14: 443-453 </p> <p>Dieffenbach, C.W dan Dveksler G. S. 2003. PCR primer: a laboratory manual. Cold Spring Harbor Laboratory Press. 520 pp </p> <p>Direktorat Jendral Perikanan Tangkap. 2012. Statistik perikanan tangkap Indonesia 2012. Kementerian Kelautan Per-ikanan </p> <p>Dunham, R.A. 20011. Aquaculture and fisheries biotechnology: Genetic Approach. CABI Publishing, Cambridge, USA. P: 85-99. </p> <p>FAO. 2010. Helostoma temminckii (Cuvier, 1829). Species fact sheet. FAO, Roma </p> <p>Falconer, F.S. dan Mackay T.F.C. 1996. Introduction to quantitative genetics. Longman England. 464pp </p> <p>Gjedrem, T. 2005. Selection and breeding programs in aquaculture. Springer. New York. P: 251-277 </p> <p>Gustiano R., Kusmini I.I., Ath-thar M.H.F. Mengenal sumber daya genetik ikan spesifik lokal air tawar indonesia untuk pengembangan budidaya. PT Penerbit IPB Press. </p> <p>Kapuscinski, A.R dan Jacobson L.D. 2007. Genetic guideline for fisheries management. University of Minnesota, Minnesota Sea Grant Collge Program. Sea Grant Research Report 17, 1-66 </p> <p>Kirpichnikov, V.S. Genetic Bases of Fish Selection. Springer Verlag Berlin. 410pp </p> <p>Nei, M and Tajima F.. 1981. DNA Polymorphism detectable by resctriction endonuclease. Genetics 97: 146-163. </p> <p>Nugroho, E., M. Takagi, N. Taniguchi. 1997. Practical manual on detection of DNA polymorphism in fish population study. Bulletin of Marine Sciences and Fisheries 17:109-129. </p> <p>Rainboth, W.L. 1996. FAO species identification field guide for fishery purposes. Fishes of the Cambodian Mekong. Rome, FAO. 265 pp. </p> <p>Tave, D. 1993. Genetics for fish hatchery managers. Kluwer Academic Publications. Netherland. 415pp </p> </div> </section> </div> <div class="entry_details"> <div class="item cover_image"> <div class="sub_item"> <a href="https://ejournalunb.ac.id/index.php/JSN/issue/view/11"> <img src="https://ejournalunb.ac.id/public/journals/2/cover_issue_11_en_US.jpg" alt=""> </a> </div> </div> <div class="item galleys"> <h2 class="pkp_screen_reader"> Downloads </h2> <ul class="value galleys_links"> <li> <a class="obj_galley_link btn btn-primary pdf" href="https://ejournalunb.ac.id/index.php/JSN/article/view/77/75"> PDF </a> </li> </ul> </div> <div class="item published"> <section class="sub_item"> <h2 class="label"> Published </h2> <div class="value"> <span>2017-12-01</span> </div> </section> </div> <div class="item citation"> <section class="sub_item citation_display"> <h2 class="label"> How to Cite </h2> <div class="value"> <div id="citationOutput" role="region" aria-live="polite"> <div class="csl-bib-body"> <div class="csl-entry">Ath-thar, M. H. F., Putriana, I., Soelistyowati, D. T., & Gustiano, R. (2017). PERFORMA GENOTIP IKAN TAMBAKAN Helostoma temminckii (CUVIER, 1829) POPULASI SUMATERA, JAWA DAN KALIMANTAN DENGAN METODE RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD). <i>JURNAL SAINS NATURAL</i>, <i>4</i>(1), 68–75. https://doi.org/10.31938/jsn.v4i1.77</div> </div> </div> <div class="citation_formats"> <button class="cmp_button citation_formats_button" aria-controls="cslCitationFormats" aria-expanded="false" data-csl-dropdown="true"> More Citation Formats </button> <div id="cslCitationFormats" class="citation_formats_list" aria-hidden="true"> <ul class="citation_formats_styles"> <li> <a aria-controls="citationOutput" href="https://ejournalunb.ac.id/index.php/JSN/citationstylelanguage/get/acm-sig-proceedings?submissionId=77&publicationId=74" data-load-citation data-json-href="https://ejournalunb.ac.id/index.php/JSN/citationstylelanguage/get/acm-sig-proceedings?submissionId=77&publicationId=74&return=json" > ACM </a> </li> <li> <a aria-controls="citationOutput" href="https://ejournalunb.ac.id/index.php/JSN/citationstylelanguage/get/acs-nano?submissionId=77&publicationId=74" data-load-citation data-json-href="https://ejournalunb.ac.id/index.php/JSN/citationstylelanguage/get/acs-nano?submissionId=77&publicationId=74&return=json" > ACS </a> </li> <li> <a aria-controls="citationOutput" href="https://ejournalunb.ac.id/index.php/JSN/citationstylelanguage/get/apa?submissionId=77&publicationId=74" data-load-citation data-json-href="https://ejournalunb.ac.id/index.php/JSN/citationstylelanguage/get/apa?submissionId=77&publicationId=74&return=json" > APA </a> </li> <li> <a aria-controls="citationOutput" href="https://ejournalunb.ac.id/index.php/JSN/citationstylelanguage/get/associacao-brasileira-de-normas-tecnicas?submissionId=77&publicationId=74" data-load-citation data-json-href="https://ejournalunb.ac.id/index.php/JSN/citationstylelanguage/get/associacao-brasileira-de-normas-tecnicas?submissionId=77&publicationId=74&return=json" > ABNT </a> </li> <li> <a aria-controls="citationOutput" href="https://ejournalunb.ac.id/index.php/JSN/citationstylelanguage/get/chicago-author-date?submissionId=77&publicationId=74" data-load-citation data-json-href="https://ejournalunb.ac.id/index.php/JSN/citationstylelanguage/get/chicago-author-date?submissionId=77&publicationId=74&return=json" > Chicago </a> </li> <li> <a aria-controls="citationOutput" href="https://ejournalunb.ac.id/index.php/JSN/citationstylelanguage/get/harvard-cite-them-right?submissionId=77&publicationId=74" data-load-citation data-json-href="https://ejournalunb.ac.id/index.php/JSN/citationstylelanguage/get/harvard-cite-them-right?submissionId=77&publicationId=74&return=json" > Harvard </a> </li> <li> <a aria-controls="citationOutput" href="https://ejournalunb.ac.id/index.php/JSN/citationstylelanguage/get/ieee?submissionId=77&publicationId=74" data-load-citation data-json-href="https://ejournalunb.ac.id/index.php/JSN/citationstylelanguage/get/ieee?submissionId=77&publicationId=74&return=json" > IEEE </a> </li> <li> <a aria-controls="citationOutput" href="https://ejournalunb.ac.id/index.php/JSN/citationstylelanguage/get/modern-language-association?submissionId=77&publicationId=74" data-load-citation data-json-href="https://ejournalunb.ac.id/index.php/JSN/citationstylelanguage/get/modern-language-association?submissionId=77&publicationId=74&return=json" > MLA </a> </li> <li> <a aria-controls="citationOutput" href="https://ejournalunb.ac.id/index.php/JSN/citationstylelanguage/get/turabian-fullnote-bibliography?submissionId=77&publicationId=74" data-load-citation data-json-href="https://ejournalunb.ac.id/index.php/JSN/citationstylelanguage/get/turabian-fullnote-bibliography?submissionId=77&publicationId=74&return=json" > Turabian </a> </li> <li> <a aria-controls="citationOutput" href="https://ejournalunb.ac.id/index.php/JSN/citationstylelanguage/get/vancouver?submissionId=77&publicationId=74" data-load-citation data-json-href="https://ejournalunb.ac.id/index.php/JSN/citationstylelanguage/get/vancouver?submissionId=77&publicationId=74&return=json" > Vancouver </a> </li> </ul> <div class="label"> Download Citation </div> <ul class="citation_formats_styles"> <li> <a href="https://ejournalunb.ac.id/index.php/JSN/citationstylelanguage/download/ris?submissionId=77&publicationId=74"> <span class="fa fa-download"></span> Endnote/Zotero/Mendeley (RIS) </a> </li> <li> <a href="https://ejournalunb.ac.id/index.php/JSN/citationstylelanguage/download/bibtex?submissionId=77&publicationId=74"> <span class="fa fa-download"></span> BibTeX </a> </li> </ul> </div> </div> </div> </section> </div> <div class="item issue"> <section class="sub_item"> <h2 class="label"> Issue </h2> <div class="value"> <a class="title" href="https://ejournalunb.ac.id/index.php/JSN/issue/view/11"> Vol. 4 No. 1 (2014): Sains Natural </a> </div> </section> <section class="sub_item"> <h2 class="label"> Section </h2> <div class="value"> Research Articles </div> </section> </div> <div class="item copyright"> <h2 class="label"> License </h2> <p>Copyright (c) 2017 M. H. Fariduddin Ath-thar, Intan Putriana, Dinar Tri Soelistyowati, Rudhy Gustiano</p> <a rel="license" href="https://creativecommons.org/licenses/by-sa/4.0/"><img alt="Creative Commons License" src="//i.creativecommons.org/l/by-sa/4.0/88x31.png" /></a><p>This work is licensed under a <a rel="license" href="https://creativecommons.org/licenses/by-sa/4.0/">Creative Commons Attribution-ShareAlike 4.0 International License</a>.</p> </div> <div class="item citations-container" data-image-path="https://ejournalunb.ac.id/plugins/generic/citations/images/" data-citations-url="https://ejournalunb.ac.id/index.php/JSN/citations/get?citationsId=10.31938%2Fjsn.v4i1.77&citationsShowList=1&citationsProvider=all" data-citations-provider="all" data-citations-total="1" data-citations-list="1" data-show-google="1" data-show-pmc="1"> <div id="citations-loader"></div> <div class="citations-count"> <div class="citations-count-crossref"> <img class="img-fluid" src="https://ejournalunb.ac.id/plugins/generic/citations/images/crossref.png" alt="Crossref"/> <div class="badge_total"></div> </div> <div class="citations-count-scopus"> <img src="https://ejournalunb.ac.id/plugins/generic/citations/images/scopus.png" alt="Scopus"/> <br/> <span class="badge_total"></span> </div> <div class="citations-count-google"> <a href="https://scholar.google.com/scholar?q=10.31938/jsn.v4i1.77" target="_blank" rel="noreferrer"> <img src="https://ejournalunb.ac.id/plugins/generic/citations/images/scholar.png" alt="Google Scholar"/> </a> </div> <div class="citations-count-pmc"> <a href="http://europepmc.org/search?scope=fulltext&query=(REF:10.31938/jsn.v4i1.77)" target="_blank" rel="noreferrer"> <img src="https://ejournalunb.ac.id/plugins/generic/citations/images/pmc.png" alt="Europe PMC"/> <br/> <span class="badge_total"></span> </a> </div> </div> <div class="citations-list"></div> </div> <link rel="stylesheet" type="text/css" href="/plugins/generic/badges/styles/badges.css"> <div class="item badges"> <h2 class="label">Metrics</h2> <div class="sub_item"> <span class="__dimensions_badge_embed__" data-doi="10.31938/jsn.v4i1.77" data-style="small_circle"></span><script async src="https://badge.dimensions.ai/badge.js" charset="utf-8"></script> </div> <div class="sub_item"> <script type='text/javascript' src='https://d1bxh8uas1mnw7.cloudfront.net/assets/embed.js'></script> <div data-badge-popover="right" data-badge-type="donut" data-doi="10.31938/jsn.v4i1.77" data-hide-no-mentions="true" class="altmetric-embed"></div> </div> <div class="sub_item"> <script type="text/javascript" src="//cdn.plu.mx/widget-popup.js"></script> <a href="https://plu.mx/plum/a/?doi=10.31938/jsn.v4i1.77" class="plumx-plum-print-popup"></a> </div> </div> </div> </div> </article> <div id="articlesBySameAuthorList"> <h3>Most read articles by the same author(s)</h3> <ul> <li> M. H. Fariduddin Ath-thar, Vitas Atmadi Prakoso, <a href="https://ejournalunb.ac.id/index.php/JSN/article/view/102"> PENGARUH PENAMBAHAN AMPELA AYAM PADA KOMPOSISI PAKAN TERHADAP PERFORMA REPRODUKSI INDUK IKAN LELE </a>, <a href="https://ejournalunb.ac.id/index.php/JSN/issue/view/13"> JURNAL SAINS NATURAL: Vol. 5 No. 1 (2015): Sains Natural </a> </li> <li> Irin Iriana Kusmini, Dinar Tri Soelistyowati, Rudhy Gustiano, Peni Pitriani, Vitas Atmadi Prakoso, <a href="https://ejournalunb.ac.id/index.php/JSN/article/view/65"> KERAGAMAN FENOTIPA IKAN NILA BEST F4,F5 DAN IKAN NILA NIRWANA 2 HASIL SELEKSI DENGAN ANALISIS TRUSS MORFOMETRIK </a>, <a href="https://ejournalunb.ac.id/index.php/JSN/issue/view/10"> JURNAL SAINS NATURAL: Vol. 3 No. 2 (2013): Sains Natural </a> </li> <li> Gleni Hasan Huwoyon, Anik Martinah Hariati, Rudhy Gustiano, <a href="https://ejournalunb.ac.id/index.php/JSN/article/view/7"> LIMBAH BIOGAS KOTORAN AYAM SEBAGAI ALTERNATIF SUMBER PROTEIN PAKAN BENIH IKAN PATIN (Pangasianodon hypophthalmus) </a>, <a href="https://ejournalunb.ac.id/index.php/JSN/issue/view/14"> JURNAL SAINS NATURAL: Vol. 1 No. 1 (2011): Sains Natural </a> </li> <li> Iskandariah Iskandariah, Otong Zenal Arifin, Rudhy Gustiano, <a href="https://ejournalunb.ac.id/index.php/JSN/article/view/8"> ANALISIS KERAGAMAN GENETIK TIGA STRAIN NILA MERAH (Oreochromis sp) DENGAN ANOVA RAPD </a>, <a href="https://ejournalunb.ac.id/index.php/JSN/issue/view/14"> JURNAL SAINS NATURAL: Vol. 1 No. 1 (2011): Sains Natural </a> </li> <li> M. H. Fariduddin Ath-thar, Dadang Ariyanto, Rudhy Gustiano, <a href="https://ejournalunb.ac.id/index.php/JSN/article/view/6"> EVALUASI PERTUMBUHAN LIMA STRAIN IKAN NILA PADA MEDIA BERSALINITAS </a>, <a href="https://ejournalunb.ac.id/index.php/JSN/issue/view/14"> JURNAL SAINS NATURAL: Vol. 1 No. 1 (2011): Sains Natural </a> </li> </ul> <div id="articlesBySameAuthorPages"> </div> </div> </div> <div class="html-preloader bg-light"> <div class="spinner-border text-primary" style="width: 3rem; height: 3rem;" role="status"> <span class="visually-hidden">Loading...</span> </div> </div> </main> <div class="mt-5 pt-4" data-aos="fade-up" data-aos-delay="200"> <ul class="_pkp_structure_sidebar list-unstyled container" role="complementary" aria-label="Sidebar"> <li> <a href="http://pkp.sfu.ca/ojs/" class="font-monospace"> Open Journal Systems </a> </li>  <div class="pkp_block block_custom" id="customblock-logo"> <h2 class="title pkp_screen_reader">logo</h2> <div class="content"> <div id="customblock-new_temp" class="pkp_block block_custom"> <div class="content"> <p><img src="/public/site/images/admin2/logo-JSNok.png" alt=""></p> <p><a href="https://ejournalunb.ac.id/index.php/JSN/about/submissions"><img src="https://jrtt.org/public/site/images/admin/submission-logo-copy.jpg" alt="" width="280" height="56"></a></p> </div> </div> </div> </div> <div class="pkp_block block_developed_by"> <div class="content"> <span class="title">Most Read</span> <ul class="most_read"> <li class="most_read_article"> <div class="most_read_article_title"><a href="https://ejournalunb.ac.id/index.php/JSN/article/view/25">GINGEROL PADA RIMPANG JAHE MERAH (Zingiber officinale, Roscoe) DENGAN METODE PERKOLASI TERMODIFIKASI BASA</a></div> <div class="most_read_article_journal"><span class="fa fa-eye"></span> 148</div> </li> <li class="most_read_article"> <div class="most_read_article_title"><a href="https://ejournalunb.ac.id/index.php/JSN/article/view/250">PENGUJIAN PARAMETER FISIK SABUN MANDI CAIR DARI SURFAKTAN SODIUM LAURETH SULFATE (SLES)</a></div> <div class="most_read_article_journal"><span class="fa fa-eye"></span> 120</div> </li> <li class="most_read_article"> <div class="most_read_article_title"><a href="https://ejournalunb.ac.id/index.php/JSN/article/view/249">ALTERNATIF METODE ANALISIS PENETAPAN KADAR ASAM MEFENAMAT DALAM PONSAMIC 500 mg TABLET SALUT SELAPUT</a></div> <div class="most_read_article_journal"><span class="fa fa-eye"></span> 119</div> </li> <li class="most_read_article"> <div class="most_read_article_title"><a href="https://ejournalunb.ac.id/index.php/JSN/article/view/32">VALIDASI METODE PENETAPAN KADAR CYANOCOBALAMIN SECARA SPEKTROFOTOMETRI VISIBEL DOUBLE BEAM</a></div> <div class="most_read_article_journal"><span class="fa fa-eye"></span> 118</div> </li> <li class="most_read_article"> <div class="most_read_article_title"><a href="https://ejournalunb.ac.id/index.php/JSN/article/view/103">HIDROLISIS ASAM KLORIDA TEPUNG PATI SINGKONG (Manihot esculenta Crantz) DALAM PEMBUATAN GULA CAIR</a></div> <div class="most_read_article_journal"><span class="fa fa-eye"></span> 117</div> </li> </ul> </div> </div> <div class="pkp_block block_custom" id="customblock-tools"> <h2 class="title pkp_screen_reader">Suggested Tools</h2> <div class="content"> <div style="padding: 8px; border-bottom: 1px solid #696969; background: #0d2455; color: #102e43;"> <p align="center"><strong><span style="font-weight: bold;"><span style="color: white;">Supported by:</span></span></strong></p> </div> <div style="padding: 8px; border-bottom: 1px solid #696969; background: white; color: darkgreen;"> <p align="center"><a style="font-size: 10px;" href="http://relawanjurnal.id"><img src="/public/site/images/admin2/rji1.png" alt=""></a></p> </div> <p align="center"> </p> <div id="customblock-new_temp" class="pkp_block block_custom"> <div class="content"> <div style="padding: 8px; border-bottom: 1px solid #696969; background: #0d2455; color: #102e43;"> <p align="center"><strong><span style="font-weight: bold;"><span style="color: white;">Suggested Tools</span></span></strong></p> </div> <div style="padding: 8px; border-bottom: 1px solid #696969; background: white; color: darkgreen;"> <p align="center"><a href="https://www.mendeley.com" target="_blank" rel="noopener"><strong><img src="https://ejournalunb.ac.id/public/site/images/admin2/preview.png" alt="" width="239" height="58"></strong></a> <strong><img src="https://ejournalunb.ac.id/public/site/images/admin2/1541574731469.png" alt="" width="123" height="58"> <a href="https://www.grammarly.com" target="_blank" rel="noopener"><img src="https://ejournalunb.ac.id/public/site/images/admin2/logo-grammarly.png" alt="" width="248" height="58"></a> <a href="https://docs.google.com/document/d/13omzg3QPd5HVgrCq2O131eLMiAXT6XfU/edit?usp=share_link&ouid=106505291203607867071&rtpof=true&sd=true" target="_blank" rel="noopener"><img src="https://ejournalunb.ac.id/public/site/images/admin2/download.png" alt="" width="190" height="58"></a></strong></p> </div> </div> </div> </div> </div> <div class="pkp_block block_custom" id="customblock-template"> <h2 class="title pkp_screen_reader">Download Template JSN</h2> <div class="content"> <div id="customblock-new_temp" class="pkp_block block_custom"> <div class="content"> <div style="padding: 8px; border-bottom: 1px solid #696969; background: #0d2455; color: #102e43;"> <p align="center"><strong><span style="font-weight: bold;"><span style="color: white;">Download Template JSN</span></span></strong></p> </div> <div style="padding: 8px; border-bottom: 1px solid #696969; background: white; color: darkgreen;"> <p align="center"><a href="https://docs.google.com/document/d/1HJBgO9qivnKeSaDchEy-JDxkXl0QpBmv/edit?usp=sharing&ouid=107018353799929461741&rtpof=true&sd=true"><strong><img src="https://ejournalunb.ac.id/public/site/images/admin2/pngtreedoc-file-document-icon-4175858.png" alt="" width="95" height="95"></strong></a></p> </div> </div> </div> </div> </div> <div class="pkp_block block_custom" id="customblock-stats"> <h2 class="title pkp_screen_reader">stats</h2> <div class="content"> <div id="customblock-new_temp" class="pkp_block block_custom"> <div class="content"> <div style="padding: 8px; border-bottom: 1px solid #696969; background: #0d2455; color: #102e43;"> <p align="center"><strong><span style="font-weight: bold;"><span style="color: white;">Visitors Statistics</span></span></strong></p> </div> <div style="padding: 8px; border-bottom: 1px solid #696969; background: white; color: darkgreen;"> <p align="center"><a href="https://info.flagcounter.com/AyCL"><img src="https://s11.flagcounter.com/count/AyCL/bg_FFFFFF/txt_000000/border_CCCCCC/columns_1/maxflags_10/viewers_0/labels_1/pageviews_0/flags_0/percent_1/" alt="Flag Counter" border="0"></a></p> </div> </div> </div> <p align="center"><a title="Web Analytics Made Easy - Statcounter" href="https://statcounter.com/" target="_blank" rel="noopener"><img src="https://c.statcounter.com/12510156/0/0690ecfa/0/" alt="Web Analytics Made Easy - Statcounter"></a></p> <p align="center"><a href="https://statcounter.com/p12510156/?guest=1">View The Statistics of J. Sains Nat.</a></p> </div> </div> <div class="pkp_block block_custom" id="customblock-indexs"> <h2 class="title pkp_screen_reader">indexs</h2> <div class="content"> <div id="customblock-new_temp" class="pkp_block block_custom"> <div class="content"> <div style="padding: 8px; border-bottom: 1px solid #696969; background: #0d2455; color: #102e43;"> <p align="center"><strong><span style="font-weight: bold;"><span style="color: white;">Jurnal Sains Natural Indexed by:</span></span></strong></p> </div> <div style="padding: 8px; border-bottom: 1px solid #696969; background: white; color: darkgreen;"> <p align="center"><a href="https://search.crossref.org/?q=jurnal+sains+natural&from_ui=yes"><img src="https://ejournalunb.ac.id/public/site/images/admin_ejournalunb/crossref-logo-landscape-2001.png" alt="" height="58"></a>  <a href="https://sinta.kemdikbud.go.id/journals/profile/7284"><img src="https://ejournalunb.ac.id/public/site/images/admin_ejournalunb/badge-sinta-journal-2.png" alt="" width="136" height="58"></a> <a title="jurnal sains natural" href="https://scholar.google.com/citations?user=MZuLzDYAAAAJ&hl=en" target="_self"><img src="https://ejournalunb.ac.id/public/site/images/admin2/scholar.png" alt="" height="58"></a> <a href="https://garuda.kemdikbud.go.id/journal/view/12405"><img src="https://ejournalunb.ac.id/public/site/images/admin2/garuda1-1900911767084fe4229f6f14713af333.png" alt="" width="249" height="58"></a></p> <p align="center"><a title="one search" href="https://onesearch.id/Search/Results?lookfor=jurnal+sains+natural&type=AllFields&sort=title&filter%5B%5D=collection%3A%22Jurnal+Sains+Natural%22" target="_self"><img src="https://ejournalunb.ac.id/public/site/images/admin2/onesearch.png" alt="" height="58"></a><a title="base" href="https://www.base-search.net/Search/Results?q=dccoll:ftuninusabangsa&refid=dcrecen"><img src="https://ejournalunb.ac.id/public/site/images/admin2/base_logo_kl.png" alt="" height="58"></a> <a href="https://app.dimensions.ai/discover/publication?search_mode=content&or_facet_source_title=jour.1338135"><img src="https://ejournalunb.ac.id/public/site/images/admin2/Dimensions-logo.png" alt="" height="58"></a> <a href="https://portal.issn.org/resource/issn/2621-508X"><img src="https://ejournalunb.ac.id/public/site/images/admin2/road-issn-4d2a8310f3299f5cf91aad7972ff781d.png" alt="" width="188" height="58"></a><a href="https://www.scilit.net/sources/93280"><img src="https://ejournalunb.ac.id/public/site/images/admin_ejournalunb/mceclip0.png" height="58"></a></p> </div> </div> </div> </div> </div> </ul> </div>  <footer class="footer" role="contentinfo"> <div class="container"> <p align="center"><strong>Publisher</strong>: Universitas Nusa Bangsa; <strong>Co-publisher</strong>: Fakultas MIPA, Universitas Nusa Bangsa; <strong>First Publication</strong>: 2011(print), 2018(online)</p> <p align="center">This work is licensed under <a style="display: inline-block;" href="http://creativecommons.org/licenses/by-sa/4.0/?ref=chooser-v1" target="_blank" rel="license noopener noreferrer">CC BY-SA 4.0<img style="height: 22px!important; margin-left: 3px; vertical-align: text-bottom;" src="https://mirrors.creativecommons.org/presskit/icons/cc.svg?ref=chooser-v1" /><img style="height: 22px!important; margin-left: 3px; vertical-align: text-bottom;" src="https://mirrors.creativecommons.org/presskit/icons/by.svg?ref=chooser-v1" /><img style="height: 22px!important; margin-left: 3px; vertical-align: text-bottom;" src="https://mirrors.creativecommons.org/presskit/icons/sa.svg?ref=chooser-v1" /></a></p> <div class="row justify-content-center text-center"> <div class="col-md-7"> <p class="copyright">© Platform & Workflow by: <a href="https://ejournalunb.ac.id/index.php/JSN/about/aboutThisPublishingSystem"> Open Journal Systems</a></p> <div class="credits"> Designed by <a href="https://github.com/madi-nuralin/material">Material Theme</a> </div> </div> </div> </div> </footer> <script src="https://ejournalunb.ac.id/lib/pkp/lib/vendor/components/jquery/jquery.min.js?v=3.3.0.12" type="text/javascript"></script><script src="https://ejournalunb.ac.id/lib/pkp/lib/vendor/components/jqueryui/jquery-ui.min.js?v=3.3.0.12" type="text/javascript"></script><script src="https://ejournalunb.ac.id/plugins/themes/material/vendor/mdb/js/mdb.min.js?v=3.3.0.12" type="text/javascript"></script><script src="https://ejournalunb.ac.id/plugins/themes/material/vendor/aos/aos.js?v=3.3.0.12" type="text/javascript"></script><script src="https://ejournalunb.ac.id/plugins/themes/material/vendor/swiper/swiper-bundle.min.js?v=3.3.0.12" type="text/javascript"></script><script src="https://ejournalunb.ac.id/plugins/themes/material/js/main.js?v=3.3.0.12" type="text/javascript"></script><script src="https://ejournalunb.ac.id/plugins/generic/citationStyleLanguage/js/articleCitation.js?v=3.3.0.12" type="text/javascript"></script><script src="https://ejournalunb.ac.id/plugins/generic/citations/js/citations.js?v=3.3.0.12" type="text/javascript"></script><script type="text/javascript">var pkpUsageStats = pkpUsageStats || {};pkpUsageStats.data = pkpUsageStats.data || {};pkpUsageStats.data.Submission = pkpUsageStats.data.Submission || {};pkpUsageStats.data.Submission[77] = {"data":{"2018":{"1":0,"2":0,"3":1,"4":1,"5":1,"6":0,"7":0,"8":0,"9":0,"10":0,"11":0,"12":0},"2019":{"1":0,"2":1,"3":2,"4":0,"5":0,"6":0,"7":0,"8":1,"9":1,"10":0,"11":0,"12":0},"2020":{"1":0,"2":0,"3":1,"4":1,"5":0,"6":0,"7":0,"8":2,"9":0,"10":1,"11":0,"12":2},"2021":{"1":2,"2":0,"3":4,"4":1,"5":0,"6":3,"7":1,"8":1,"9":2,"10":1,"11":0,"12":1},"2022":{"1":0,"2":1,"3":0,"4":5,"5":1,"6":0,"7":2,"8":0,"9":2,"10":1,"11":0,"12":1},"2023":{"1":1,"2":13,"3":48,"4":15,"5":14,"6":4,"7":6,"8":4,"9":60,"10":23,"11":16,"12":4},"2024":{"1":1,"2":8,"3":17,"4":7,"5":26,"6":26,"7":12,"8":11,"9":123,"10":87,"11":36,"12":49},"2025":{"1":49,"2":32,"3":1,"4":0,"5":0,"6":0,"7":0,"8":0,"9":0,"10":0,"11":0,"12":0}},"label":"All Downloads","color":"79,181,217","total":737};</script><script src="https://cdnjs.cloudflare.com/ajax/libs/Chart.js/2.0.1/Chart.js?v=3.3.0.12" type="text/javascript"></script><script type="text/javascript">var pkpUsageStats = pkpUsageStats || {};pkpUsageStats.locale = pkpUsageStats.locale || {};pkpUsageStats.locale.months = ["Jan","Feb","Mar","Apr","May","Jun","Jul","Aug","Sep","Oct","Nov","Dec"];pkpUsageStats.config = pkpUsageStats.config || {};pkpUsageStats.config.chartType = "bar";</script><script src="https://ejournalunb.ac.id/plugins/generic/usageStats/js/UsageStatsFrontendHandler.js?v=3.3.0.12" type="text/javascript"></script> </body> </html>

CINXE.COM

PERFORMA GENOTIP IKAN TAMBAKAN Helostoma temminckii (CUVIER, 1829) POPULASI SUMATERA, JAWA DAN KALIMANTAN DENGAN METODE RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) | JURNAL SAINS NATURAL