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Everardo Olguín Tiznado</title> <meta name="description" content="Search results for: Jesús Everardo Olguín Tiznado"> <meta name="keywords" content="Jesús Everardo Olguín Tiznado"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> 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action="https://publications.waset.org/abstracts/search"> <div id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="Jesús Everardo Olguín Tiznado"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 126</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: Jesús Everardo Olguín Tiznado</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">6</span> Enhancing Seismic Resilience in Colombia's Informal Housing: A Low-cost Retrofit Strategy with Buckling-restrained Braces to Protect Vulnerable Communities in Earthquake-prone Regions</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Luis%20F.%20Caballero-castro">Luis F. Caballero-castro</a>, <a href="https://publications.waset.org/abstracts/search?q=Dirsa%20Feliciano"> Dirsa Feliciano</a>, <a href="https://publications.waset.org/abstracts/search?q=Daniela%20Novoa"> Daniela Novoa</a>, <a href="https://publications.waset.org/abstracts/search?q=Orlando%20Arroyo"> Orlando Arroyo</a>, <a href="https://publications.waset.org/abstracts/search?q=Jes%C3%BAs%20D.%20Villalba-morales"> Jesús D. Villalba-morales</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Colombia faces a critical challenge in seismic resilience due to the prevalence of informal housing, which constitutes approximately 70% of residential structures. More than 10 million Colombians (20% of the population), live in homes susceptible to collapse in the event of an earthquake. This, combined with the fact that 83% of the population is in intermediate and high seismic hazard areas, has brought serious consequences to the country. These consequences became evident during the 1999 Armenia earthquake, which affected nearly 100,000 properties and represented economic losses equivalent to 1.88% of that year's Gross Domestic Product (GDP). Despite previous efforts to reinforce informal housing through methods like externally reinforced masonry walls, alternatives related to seismic protection systems (SPDs), such as Buckling-Restrained Braces (BRB), have not yet been explored in the country. BRBs are reinforcement elements capable of withstanding both compression and tension, making them effective in enhancing the lateral stiffness of structures. In this study, the use of low-cost and easily installable BRBs for the retrofit of informal housing in Colombia was evaluated, considering the economic limitations of the communities. For this purpose, a case study was selected involving an informally constructed dwelling in the country, from which field information on its structural characteristics and construction materials was collected. Based on the gathered information, nonlinear models with and without BRBs were created, and their seismic performance was analyzed and compared through incremental static (pushover) and nonlinear dynamic analyses. In the first analysis, the capacity curve was identified, showcasing the sequence of failure events occurring from initial yielding to structural collapse. In the second case, the model underwent nonlinear dynamic analyses using a set of seismic records consistent with the country's seismic hazard. Based on the results, fragility curves were calculated to evaluate the probability of failure of the informal housings before and after the intervention with BRBs, providing essential information about their effectiveness in reducing seismic vulnerability. The results indicate that low-cost BRBs can significantly increase the capacity of informal housing to withstand earthquakes. The dynamic analysis revealed that retrofit structures experienced lower displacements and deformations, enhancing the safety of residents and the seismic performance of informally constructed houses. In other words, the use of low-cost BRBs in the retrofit of informal housing in Colombia is a promising strategy for improving structural safety in seismic-prone areas. This study emphasizes the importance of seeking affordable and practical solutions to address seismic risk in vulnerable communities in earthquake-prone regions in Colombia and serves as a model for addressing similar challenges of informal housing worldwide. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=buckling-restrained%20braces" title="buckling-restrained braces">buckling-restrained braces</a>, <a href="https://publications.waset.org/abstracts/search?q=fragility%20curves" title=" fragility curves"> fragility curves</a>, <a href="https://publications.waset.org/abstracts/search?q=informal%20housing" title=" informal housing"> informal housing</a>, <a href="https://publications.waset.org/abstracts/search?q=incremental%20dynamic%20analysis" title=" incremental dynamic analysis"> incremental dynamic analysis</a>, <a href="https://publications.waset.org/abstracts/search?q=seismic%20retrofit" title=" seismic retrofit"> seismic retrofit</a> </p> <a href="https://publications.waset.org/abstracts/173797/enhancing-seismic-resilience-in-colombias-informal-housing-a-low-cost-retrofit-strategy-with-buckling-restrained-braces-to-protect-vulnerable-communities-in-earthquake-prone-regions" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/173797.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">96</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">5</span> Hydraulic Headloss in Plastic Drainage Pipes at Full and Partially Full Flow</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Velitchko%20G.%20Tzatchkov">Velitchko G. Tzatchkov</a>, <a href="https://publications.waset.org/abstracts/search?q=Petronilo%20E.%20Cortes-Mejia"> Petronilo E. Cortes-Mejia</a>, <a href="https://publications.waset.org/abstracts/search?q=J.%20Manuel%20Rodriguez-Varela"> J. Manuel Rodriguez-Varela</a>, <a href="https://publications.waset.org/abstracts/search?q=Jesus%20Figueroa-Vazquez"> Jesus Figueroa-Vazquez</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Hydraulic headloss, expressed by the values of friction factor f and Manning’s coefficient n, is an important parameter in designing drainage pipes. Their values normally are taken from manufacturer recommendations, many times without sufficient experimental support. To our knowledge, currently there is no standard procedure for hydraulically testing such pipes. As a result of research carried out at the Mexican Institute of Water Technology, a laboratory testing procedure was proposed and applied on 6 and 12 inches diameter polyvinyl chloride (PVC) and high-density dual wall polyethylene pipe (HDPE) drainage pipes. While the PVC pipe is characterized by naturally smooth interior and exterior walls, the dual wall HDPE pipe has corrugated exterior wall and, although considered smooth, a slightly wavy interior wall. The pipes were tested at full and partially full pipe flow conditions. The tests for full pipe flow were carried out on a 31.47 m long pipe at flow velocities between 0.11 and 4.61 m/s. Water was supplied by gravity from a 10 m-high tank in some of the tests, and from a 3.20 m-high tank in the rest of the tests. Pressure was measured independently with piezometer readings and pressure transducers. The flow rate was measured by an ultrasonic meter. For the partially full pipe flow the pipe was placed inside an existing 49.63 m long zero slope (horizontal) channel. The flow depth was measured by piezometers located along the pipe, for flow rates between 2.84 and 35.65 L/s, measured by a rectangular weir. The observed flow profiles were then compared to computer generated theoretical gradually varied flow profiles for different Manning’s n values. It was found that Manning’s n, that normally is assumed constant for a given pipe material, is in fact dependent on flow velocity and pipe diameter for full pipe flow, and on flow depth for partially full pipe flow. Contrary to the expected higher values of n and f for the HDPE pipe, virtually the same values were obtained for the smooth interior wall PVC pipe and the slightly wavy interior wall HDPE pipe. The explanation of this fact was found in Henry Morris’ theory for smooth turbulent conduit flow over isolated roughness elements. Following Morris, three categories of the flow regimes are possible in a rough conduit: isolated roughness (or semi smooth turbulent) flow, wake interference (or hyper turbulent) flow, and skimming (or quasi-smooth) flow. Isolated roughness flow is characterized by friction drag turbulence over the wall between the roughness elements, independent vortex generation, and dissipation around each roughness element. In this regime, the wake and vortex generation zones at each element develop and dissipate before attaining the next element. The longitudinal spacing of the roughness elements and their height are important influencing agents. Given the slightly wavy form of the HDPE pipe interior wall, the flow for this type of pipe belongs to this category. Based on that theory, an equation for the hydraulic friction factor was obtained. The obtained coefficient values are going to be used in the Mexican design standards. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=drainage%20plastic%20pipes" title="drainage plastic pipes">drainage plastic pipes</a>, <a href="https://publications.waset.org/abstracts/search?q=hydraulic%20headloss" title=" hydraulic headloss"> hydraulic headloss</a>, <a href="https://publications.waset.org/abstracts/search?q=hydraulic%20friction%20factor" title=" hydraulic friction factor"> hydraulic friction factor</a>, <a href="https://publications.waset.org/abstracts/search?q=Manning%E2%80%99s%20n" title=" Manning’s n"> Manning’s n</a> </p> <a href="https://publications.waset.org/abstracts/70867/hydraulic-headloss-in-plastic-drainage-pipes-at-full-and-partially-full-flow" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/70867.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">281</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4</span> Chain Networks on Internationalization of SMEs: Co-Opetition Strategies in Agrifood Sector</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Emilio%20Galdeano-G%C3%B3mez">Emilio Galdeano-Gómez</a>, <a href="https://publications.waset.org/abstracts/search?q=Juan%20C.%20P%C3%A9rez-Mesa"> Juan C. Pérez-Mesa</a>, <a href="https://publications.waset.org/abstracts/search?q=Laura%20Piedra-Mu%C3%B1oz"> Laura Piedra-Muñoz</a>, <a href="https://publications.waset.org/abstracts/search?q=Mar%C3%ADa%20C.%20Garc%C3%ADa-Barranco"> María C. García-Barranco</a>, <a href="https://publications.waset.org/abstracts/search?q=Jes%C3%BAs%20Hern%C3%A1ndez-Rubio"> Jesús Hernández-Rubio</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The situation in which firms engage in simultaneous cooperation and competition with each other is a phenomenon known as co-opetition. This scenario has received increasing attention in business economics and management analyses. In the domain of supply chain networks and for small and medium-sized enterprises, SMEs, these strategies are of greater relevance given the complex environment of globalization and competition in open markets. These firms face greater challenges regarding technology and access to specific resources due to their limited capabilities and limited market presence. Consequently, alliances and collaborations with both buyers and suppliers prove to be key elements in overcoming these constraints. However, rivalry and competition are also regarded as major factors in successful internationalization processes, as they are drivers for firms to attain a greater degree of specialization and to improve efficiency, for example enabling them to allocate scarce resources optimally and providing incentives for innovation and entrepreneurship. The present work aims to contribute to the literature on SMEs’ internationalization strategies. The sample is constituted by a panel data of marketing firms from the Andalusian food sector and a multivariate regression analysis is developed, measuring variables of co-opetition and international activity. The hierarchical regression equations method has been followed, thus resulting in three estimated models: the first one excluding the variables indicative of channel type, while the latter two include the international retailer chain and wholesaler variable. The findings show that the combination of several factors leads to a complex scenario of inter-organizational relationships of cooperation and competition. In supply chain management analyses, these relationships tend to be classified as either buyer-supplier (vertical level) or supplier-supplier relationships (horizontal level). Several buyers and suppliers tend to participate in supply chain networks, and in which the form of governance (hierarchical and non-hierarchical) influences cooperation and competition strategies. For instance, due to their market power and/or their closeness to the end consumer, some buyers (e.g. large retailers in food markets) can exert an influence on the selection and interaction of several of their intermediate suppliers, thus endowing certain networks in the supply chain with greater stability. This hierarchical influence may in turn allow these suppliers to develop their capabilities (e.g. specialization) to a greater extent. On the other hand, for those suppliers that are outside these networks, this environment of hierarchy, characterized by a “hub firm” or “channel master”, may provide an incentive for developing their co-opetition relationships. These results prove that the analyzed firms have experienced considerable growth in sales to new foreign markets, mainly in Europe, dealing with large retail chains and wholesalers as main buyers. This supply industry is predominantly made up of numerous SMEs, which has implied a certain disadvantage when dealing with the buyers, as negotiations have traditionally been held on an individual basis and in the face of high competition among suppliers. Over recent years, however, cooperation among these marketing firms has become more common, for example regarding R&D, promotion, scheduling of production and sales. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=co-petition%20networks" title="co-petition networks">co-petition networks</a>, <a href="https://publications.waset.org/abstracts/search?q=international%20supply%20chain" title=" international supply chain"> international supply chain</a>, <a href="https://publications.waset.org/abstracts/search?q=maketing%20agrifood%20firms" title=" maketing agrifood firms"> maketing agrifood firms</a>, <a href="https://publications.waset.org/abstracts/search?q=SMEs%20strategies" title=" SMEs strategies"> SMEs strategies</a> </p> <a href="https://publications.waset.org/abstracts/148877/chain-networks-on-internationalization-of-smes-co-opetition-strategies-in-agrifood-sector" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/148877.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">79</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3</span> A Tool to Provide Advanced Secure Exchange of Electronic Documents through Europe</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jesus%20Carretero">Jesus Carretero</a>, <a href="https://publications.waset.org/abstracts/search?q=Mario%20Vasile"> Mario Vasile</a>, <a href="https://publications.waset.org/abstracts/search?q=Javier%20Garcia-Blas"> Javier Garcia-Blas</a>, <a href="https://publications.waset.org/abstracts/search?q=Felix%20Garcia-Carballeira"> Felix Garcia-Carballeira</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Supporting cross-border secure and reliable exchange of data and documents and to promote data interoperability is critical for Europe to enhance sector (like eFinance, eJustice and eHealth). This work presents the status and results of the European Project MADE, a Research Project funded by Connecting Europe facility Programme, to provide secure e-invoicing and e-document exchange systems among Europe countries in compliance with the eIDAS Regulation (Regulation EU 910/2014 on electronic identification and trust services). The main goal of MADE is to develop six new AS4 Access Points and SMP in Europe to provide secure document exchanges using the eDelivery DSI (Digital Service Infrastructure) amongst both private and public entities. Moreover, the project demonstrates the feasibility and interest of the solution provided by providing several months of interoperability among the providers of the six partners in different EU countries. To achieve those goals, we have followed a methodology setting first a common background for requirements in the partner countries and the European regulations. Then, the partners have implemented access points in each country, including their service metadata publisher (SMP), to allow the access to their clients to the pan-European network. Finally, we have setup interoperability tests with the other access points of the consortium. The tests will include the use of each entity production-ready Information Systems that process the data to confirm all steps of the data exchange. For the access points, we have chosen AS4 instead of other existing alternatives because it supports multiple payloads, native web services, pulling facilities, lightweight client implementations, modern crypto algorithms, and more authentication types, like username-password and X.509 authentication and SAML authentication. The main contribution of MADE project is to open the path for European companies to use eDelivery services with cross-border exchange of electronic documents following PEPPOL (Pan-European Public Procurement Online) based on the e-SENS AS4 Profile. It also includes the development/integration of new components, integration of new and existing logging and traceability solutions and maintenance tool support for PKI. Moreover, we have found that most companies are still not ready to support those profiles. Thus further efforts will be needed to promote this technology into the companies. The consortium includes the following 9 partners. From them, 2 are research institutions: University Carlos III of Madrid (Coordinator), and Universidad Politecnica de Valencia. The other 7 (EDICOM, BIZbrains, Officient, Aksesspunkt Norge, eConnect, LMT group, Unimaze) are private entities specialized in secure delivery of electronic documents and information integration brokerage in their respective countries. To achieve cross-border operativity, they will include AS4 and SMP services in their platforms according to the EU Core Service Platform. Made project is instrumental to test the feasibility of cross-border documents eDelivery in Europe. If successful, not only einvoices, but many other types of documents will be securely exchanged through Europe. It will be the base to extend the network to the whole Europe. This project has been funded under the Connecting Europe Facility Agreement number: INEA/CEF/ICT/A2016/1278042. Action No: 2016-EU-IA-0063. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=security" title="security">security</a>, <a href="https://publications.waset.org/abstracts/search?q=e-delivery" title=" e-delivery"> e-delivery</a>, <a href="https://publications.waset.org/abstracts/search?q=e-invoicing" title=" e-invoicing"> e-invoicing</a>, <a href="https://publications.waset.org/abstracts/search?q=e-delivery" title=" e-delivery"> e-delivery</a>, <a href="https://publications.waset.org/abstracts/search?q=e-document%20exchange" title=" e-document exchange"> e-document exchange</a>, <a href="https://publications.waset.org/abstracts/search?q=trust" title=" trust"> trust</a> </p> <a href="https://publications.waset.org/abstracts/95782/a-tool-to-provide-advanced-secure-exchange-of-electronic-documents-through-europe" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/95782.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">265</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2</span> Biochemical and Antiviral Study of Peptides Isolated from Amaranthus hypochondriacus on Tomato Yellow Leaf Curl Virus Replication</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jos%C3%A9%20Silvestre%20Mendoza%20Figueroa">José Silvestre Mendoza Figueroa</a>, <a href="https://publications.waset.org/abstracts/search?q=Anders%20Kvarnheden"> Anders Kvarnheden</a>, <a href="https://publications.waset.org/abstracts/search?q=Jes%C3%BAs%20M%C3%A9ndez%20Lozano"> Jesús Méndez Lozano</a>, <a href="https://publications.waset.org/abstracts/search?q=Edgar%20Antonio%20Rodr%C3%ADguez%20Negrete"> Edgar Antonio Rodríguez Negrete</a>, <a href="https://publications.waset.org/abstracts/search?q=Manuel%20Soriano%20Garc%C3%ADa"> Manuel Soriano García</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Agroindustrial plants such as cereals and pseudo cereals offer a substantial source of biomacromolecules, as they contain large amounts per tissue-gram of proteins, polysaccharides and lipids in comparison with other plants. In particular, Amaranthus hypochondriacus seeds have high levels of proteins in comparison with other cereal and pseudo cereal species, which makes the plant a good source of bioactive molecules such as peptides. Geminiviruses are one principal class of pathogens that causes important economic losses in crops, affecting directly the development and production of the plant. One such virus is the Tomato yellow leaf curl virus (TYLCV), which affects mainly Solanacea family plants such as tomato species. The symptoms of the disease are curling of leaves, chlorosis, dwarfing and floral abortion. The aim of this work was to get peptides derived from enzymatic hydrolysis of globulins and albumins from amaranth seeds with specific recognition of the replication origin in the TYLCV genome, and to test the antiviral activity on host plants with the idea to generate a direct control of this viral infection. Globulins and albumins from amaranth were extracted, the fraction was enzymatically digested with papain, and the aromatic peptides fraction was selected for further purification. Six peptides were tested against the replication origin (OR) using affinity assays, surface resonance plasmon and fluorescent titration, and two of these peptides showed high affinity values to the replication origin of the virus, dissociation constant values were calculated and showed specific interaction between the peptide Ampep1 and the OR. An in vitro replication test of the total TYLCV DNA was performed, in which the peptide AmPep1 was added in different concentrations to the system reaction, which resulted in a decrease of viral DNA synthesis when the peptide concentration increased. Also, we showed that the peptide can decrease the complementary DNA chain of the virus in Nicotiana benthamiana leaves, confirming that the peptide binds to the OR and that its expected mechanism of action is to decrease the replication rate of the viral genome. In an infection assay, N. benthamiana plants were agroinfected with TYLCV-Israel and TYLCV-Guasave. After confirming systemic infection, the peptide was infiltrated in new infected leaves, and the plants treated with the peptide showed a decrease of virus symptoms and viral titer. In order to confirm the antiviral activity in a commercial crop, tomato plants were infected with TYLCV. After confirming systemic infection, plants were infiltrated with peptide solution as above, and the symptom development was monitored 21 days after treatment, showing that tomato plants treated with peptides had lower symptom rates and viral titer. The peptide was also tested against other begomovirus such as Pepper huasteco yellow vein virus (PHYVV-Guasave), showing a decrease of symptoms in N. benthamiana infected plants. The model of direct biochemical control of TYLCV infection shown in this work can be extrapolated to other begomovirus infections, and the methods reported here can be used for design of antiviral agrochemicals for other plant virus infections. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=agrochemical%20screening" title="agrochemical screening">agrochemical screening</a>, <a href="https://publications.waset.org/abstracts/search?q=antiviral" title=" antiviral"> antiviral</a>, <a href="https://publications.waset.org/abstracts/search?q=begomovirus" title=" begomovirus"> begomovirus</a>, <a href="https://publications.waset.org/abstracts/search?q=geminivirus" title=" geminivirus"> geminivirus</a>, <a href="https://publications.waset.org/abstracts/search?q=peptides" title=" peptides"> peptides</a>, <a href="https://publications.waset.org/abstracts/search?q=plasmon" title=" plasmon"> plasmon</a>, <a href="https://publications.waset.org/abstracts/search?q=TYLCV" title=" TYLCV"> TYLCV</a> </p> <a href="https://publications.waset.org/abstracts/73010/biochemical-and-antiviral-study-of-peptides-isolated-from-amaranthus-hypochondriacus-on-tomato-yellow-leaf-curl-virus-replication" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/73010.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">276</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1</span> Design of DNA Origami Structures Using LAMP Products as a Combined System for the Detection of Extended Spectrum B-Lactamases</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Kalaumari%20Mayoral-Pe%C3%B1a">Kalaumari Mayoral-Peña</a>, <a href="https://publications.waset.org/abstracts/search?q=Ana%20I.%20Montejano-Montelongo"> Ana I. Montejano-Montelongo</a>, <a href="https://publications.waset.org/abstracts/search?q=Josu%C3%A9%20Reyes-Mu%C3%B1oz"> Josué Reyes-Muñoz</a>, <a href="https://publications.waset.org/abstracts/search?q=Gonzalo%20A.%20Ortiz-Mancilla"> Gonzalo A. Ortiz-Mancilla</a>, <a href="https://publications.waset.org/abstracts/search?q=Mayrin%20Rodr%C3%ADguez-Cruz"> Mayrin Rodríguez-Cruz</a>, <a href="https://publications.waset.org/abstracts/search?q=V%C3%ADctor%20Hern%C3%A1ndez-Villalobos"> Víctor Hernández-Villalobos</a>, <a href="https://publications.waset.org/abstracts/search?q=Jes%C3%BAs%20A.%20Guzm%C3%A1n-L%C3%B3pez"> Jesús A. Guzmán-López</a>, <a href="https://publications.waset.org/abstracts/search?q=Santiago%20Garc%C3%ADa-Jacobo"> Santiago García-Jacobo</a>, <a href="https://publications.waset.org/abstracts/search?q=Iv%C3%A1n%20Licona-V%C3%A1zquez"> Iván Licona-Vázquez</a>, <a href="https://publications.waset.org/abstracts/search?q=Grisel%20Fierros-Romero"> Grisel Fierros-Romero</a>, <a href="https://publications.waset.org/abstracts/search?q=Rosario%20Flores-Vallejo"> Rosario Flores-Vallejo</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The group B-lactamic antibiotics include some of the most frequently used small drug molecules against bacterial infections. Nevertheless, an alarming decrease in their efficacy has been reported due to the emergence of antibiotic-resistant bacteria. Infections caused by bacteria expressing extended Spectrum B-lactamases (ESBLs) are difficult to treat and account for higher morbidity and mortality rates, delayed recovery, and high economic burden. According to the Global Report on Antimicrobial Resistance Surveillance, it is estimated that mortality due to resistant bacteria will ascend to 10 million cases per year worldwide. These facts highlight the importance of developing low-cost and readily accessible detection methods of drug-resistant ESBLs bacteria to prevent their spread and promote accurate and fast diagnosis. Bacterial detection is commonly done using molecular diagnostic techniques, where PCR stands out for its high performance. However, this technique requires specialized equipment not available everywhere, is time-consuming, and has a high cost. Loop-Mediated Isothermal Amplification (LAMP) is an alternative technique that works at a constant temperature, significantly decreasing the equipment cost. It yields double-stranded DNA of several lengths with repetitions of the target DNA sequence as a product. Although positive and negative results from LAMP can be discriminated by colorimetry, fluorescence, and turbidity, there is still a large room for improvement in the point-of-care implementation. DNA origami is a technique that allows the formation of 3D nanometric structures by folding a large single-stranded DNA (scaffold) into a determined shape with the help of short DNA sequences (staples), which hybridize with the scaffold. This research aimed to generate DNA origami structures using LAMP products as scaffolds to improve the sensitivity to detect ESBLs in point-of-care diagnosis. For this study, the coding sequence of the CTM-X-15 ESBL of E. coli was used to generate the LAMP products. The set of LAMP primers were designed using PrimerExplorerV5. As a result, a target sequence of 200 nucleotides from CTM-X-15 ESBL was obtained. Afterward, eight different DNA origami structures were designed using the target sequence in the SDCadnano and analyzed with CanDo to evaluate the stability of the 3D structures. The designs were constructed minimizing the total number of staples to reduce costs and complexity for point-of-care applications. After analyzing the DNA origami designs, two structures were selected. The first one was a zig-zag flat structure, while the second one was a wall-like shape. Given the sequence repetitions in the scaffold sequence, both were able to be assembled with only 6 different staples each one, ranging between 18 to 80 nucleotides. Simulations of both structures were performed using scaffolds of different sizes yielding stable structures in all the cases. The generation of the LAMP products were tested by colorimetry and electrophoresis. The formation of the DNA structures was analyzed using electrophoresis and colorimetry. The modeling of novel detection methods through bioinformatics tools allows reliable control and prediction of results. To our knowledge, this is the first study that uses LAMP products and DNA-origami in combination to delect ESBL-producing bacterial strains, which represent a promising methodology for diagnosis in the point-of-care. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=beta-lactamases" title="beta-lactamases">beta-lactamases</a>, <a href="https://publications.waset.org/abstracts/search?q=antibiotic%20resistance" title=" antibiotic resistance"> antibiotic resistance</a>, <a href="https://publications.waset.org/abstracts/search?q=DNA%20origami" title=" DNA origami"> DNA origami</a>, <a href="https://publications.waset.org/abstracts/search?q=isothermal%20amplification" title=" isothermal amplification"> isothermal amplification</a>, <a href="https://publications.waset.org/abstracts/search?q=LAMP%20technique" title=" LAMP technique"> LAMP technique</a>, <a href="https://publications.waset.org/abstracts/search?q=molecular%20diagnosis" title=" molecular diagnosis"> molecular diagnosis</a> </p> <a href="https://publications.waset.org/abstracts/142416/design-of-dna-origami-structures-using-lamp-products-as-a-combined-system-for-the-detection-of-extended-spectrum-b-lactamases" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/142416.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">222</span> </span> </div> </div> <ul class="pagination"> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=Jes%C3%BAs%20Everardo%20Olgu%C3%ADn%20Tiznado&page=4" rel="prev">&lsaquo;</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=Jes%C3%BAs%20Everardo%20Olgu%C3%ADn%20Tiznado&page=1">1</a></li> <li class="page-item"><a class="page-link" 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