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class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 5</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: Kai-Frederic Seitz</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">5</span> Developing a Cybernetic Model of Interdepartmental Logistic Interactions in SME</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jonas%20Mayer">Jonas Mayer</a>, <a href="https://publications.waset.org/abstracts/search?q=Kai-Frederic%20Seitz"> Kai-Frederic Seitz</a>, <a href="https://publications.waset.org/abstracts/search?q=Thorben%20Kuprat"> Thorben Kuprat</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In today’s competitive environment production’s logistic objectives such as ‘delivery reliability’ and ‘delivery time’ and distribution’s logistic objectives such as ‘service level’ and ‘delivery delay’ are attributed great importance. Especially for small and mid-sized enterprises (SME) attaining these objectives pose a key challenge. Within this context, one of the difficulties is that interactions between departments within the enterprise and their specific objectives are insufficiently taken into account and aligned. Interdepartmental independencies along with contradicting targets set within the different departments result in enterprises having sub-optimal logistic performance capability. This paper presents a research project which will systematically describe the interactions between departments and convert them into a quantifiable form. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=department-specific%20actuating%20and%20control%20variables" title="department-specific actuating and control variables">department-specific actuating and control variables</a>, <a href="https://publications.waset.org/abstracts/search?q=interdepartmental%20interactions" title=" interdepartmental interactions"> interdepartmental interactions</a>, <a href="https://publications.waset.org/abstracts/search?q=cybernetic%20model" title=" cybernetic model"> cybernetic model</a>, <a href="https://publications.waset.org/abstracts/search?q=logistic%20objectives" title=" logistic objectives"> logistic objectives</a> </p> <a href="https://publications.waset.org/abstracts/10592/developing-a-cybernetic-model-of-interdepartmental-logistic-interactions-in-sme" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/10592.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">378</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4</span> Topical Nonsteroidal Anti-Inflammatory Eye Drops and Oral Acetazolamide for Macular Edema after Uncomplicated Phacoemulsification: Outcome and Predictors of Non-Response</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Wissam%20Aljundi">Wissam Aljundi</a>, <a href="https://publications.waset.org/abstracts/search?q=Loay%20Daas"> Loay Daas</a>, <a href="https://publications.waset.org/abstracts/search?q=Yaser%20Abu%20Dail"> Yaser Abu Dail</a>, <a href="https://publications.waset.org/abstracts/search?q=Barbara%20K%C3%A4smann-Kellner"> Barbara Käsmann-Kellner</a>, <a href="https://publications.waset.org/abstracts/search?q=Berthold%20Seitz"> Berthold Seitz</a>, <a href="https://publications.waset.org/abstracts/search?q=Alaa%20Din%20Abdin"> Alaa Din Abdin</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Purpose: To investigate the effectiveness of nonsteroidal anti-inflammatory eye drops (NSAIDs) combined with oral acetazolamide for postoperative macular edema (PME) after uncomplicated phacoemulsification (PE) and to identify predictors of non-response. Methods: We analyzed data of uncomplicated PE and identified eyes with PME. First-line therapy included topical NSAIDs combined with oral acetazolamide. In case of non-response, triamcinolone was administered subtenonally. Outcome measures included best-corrected visual acuity (BCVA) and central macular thickness (CMT). Results: 94 eyes out of 9750 uncomplicated PE developed PME, of which 60 eyes were included. Follow-ups occurred 6.4±1.8, 12.5±3.7, and 18.6±6.0 weeks after diagnosis. BCVA and CMT improved significantly in all follow-ups. 40 eyes showed response to first-line therapy at first follow-up (G1). The remaining 20 eyes showed no response and required subtenon triamcinolone (G2), of which 11 eyes showed complete regression at the second follow-up and 4 eyes at the third follow-up. 5 eyes showed no response and required intravitreal injection. Multivariate linear regression model showed that diabetes mellitus (DM) and increased cumulative dissipated energy (CDE) are predictors of non-response. Conclusion: Topical NSAIDs with acetazolamide resulted in complete regression of PME in 67% of all cases. DM and increased CDE might be considered as predictors of nonresponse to this treatment. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=postoperative%20macular%20edema" title="postoperative macular edema">postoperative macular edema</a>, <a href="https://publications.waset.org/abstracts/search?q=intravitreal%20injection" title=" intravitreal injection"> intravitreal injection</a>, <a href="https://publications.waset.org/abstracts/search?q=cumulative%20energy" title=" cumulative energy"> cumulative energy</a>, <a href="https://publications.waset.org/abstracts/search?q=irvine%20gass%20syndrome" title=" irvine gass syndrome"> irvine gass syndrome</a>, <a href="https://publications.waset.org/abstracts/search?q=pseudophakie" title=" pseudophakie"> pseudophakie</a> </p> <a href="https://publications.waset.org/abstracts/156170/topical-nonsteroidal-anti-inflammatory-eye-drops-and-oral-acetazolamide-for-macular-edema-after-uncomplicated-phacoemulsification-outcome-and-predictors-of-non-response" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/156170.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">124</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3</span> Apoptosis and Alterations in P21 and P27 Levels in Human Primary Aniridia Limbal Stromal Cells, in an Lps-Induced Inflammatory Microenvironment, in Vitro</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Shanhe%20Liu">Shanhe Liu</a>, <a href="https://publications.waset.org/abstracts/search?q=Shuailin%20Li"> Shuailin Li</a>, <a href="https://publications.waset.org/abstracts/search?q=Shao-Lun%20Hsu"> Shao-Lun Hsu</a>, <a href="https://publications.waset.org/abstracts/search?q=Berthold%20Seitz"> Berthold Seitz</a>, <a href="https://publications.waset.org/abstracts/search?q=Shweta%20Suiwal"> Shweta Suiwal</a>, <a href="https://publications.waset.org/abstracts/search?q=Tanja%20Stachon"> Tanja Stachon</a>, <a href="https://publications.waset.org/abstracts/search?q=N%C3%B3ra%20Szentm%C3%A1ry"> Nóra Szentmáry</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Purpose: Congenital aniridia is a rare ocular disorder with partial or complete absence of the iris in most cases and is frequently accompanied by aniridia-associated keratopathy (AAK). Evidence from prior studies suggests increased susceptibility of corneal limbal stromal cells to inflammatory stimuli, in which an increased apoptotic rate may play a significant role. This study aimed to investigate apoptosis in primary aniridia limbal stromal cells and to assess changes in p21 and p27 levels in response to lipopolysaccharide (LPS)-induced inflammation in vitro. Methods: Primary human corneal fibroblasts were isolated from the limbal region of both aniridia (AN-LSCs; n=8) and healthy (LSCs; n=8) donors. The cells were treated with 0 µg/ml, 2.5 µg/ml, 10 µg/ml and 17.5 µg/ml LPS for 24 hours. Apoptosis was assessed by flow cytometry in each group. The expression levels of apoptosis-related genes CDKN1A (p21) and CDKN1B (p27) were measured by qPCR. p21 and p27 protein levels were analyzed by flow cytometry. Results: Flow cytometry revealed a significantly higher apoptotic rate in AN-LSCs, than in LSCs (p<0.0001). CDKN1A mRNA level and p21 protein level were significantly higher in AN-LSCs than in LSCs (p=0.0232, p=0.0003). In AN-LSCs, 17.5 µg/ml LPS treatment significantly increased the apoptotic rate (p=0.0007) but had no effect on the apoptotic rate in LSCs (p>0.05). In LSCs, 10 and 17.5 µg/ml LPS treatment significantly increased CDKN1B mRNA levels (p=0.0028, p=0.0019) without changes in p27 protein levels (p>0.05). In AN-LFC, all LPS concentrations significantly increased CDKN1B mRNA levels (p≤0.0051) without changes at the protein level (p>0.05). Conclusions: There is an increased apoptotic rate in limbal stromal cells of congenital aniridia patients, which is accompanied by an increased p21 protein level. AN-LSCs are more sensible to LPS-induced inflammation than normal controls, and LPS treatment triggers CDKN1B mRNA levels both in AN-LSCs and LSCs Further studies should clarify the specific changes in the apoptotic cascade and identify potential therapeutic targets in limbal stromal cells of patients with congenital aniridia, aiming to prevent or delay the progression of AAK. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=limbal%20fibroblasts" title="limbal fibroblasts">limbal fibroblasts</a>, <a href="https://publications.waset.org/abstracts/search?q=aniridia%20associated%20keratopathy" title=" aniridia associated keratopathy"> aniridia associated keratopathy</a>, <a href="https://publications.waset.org/abstracts/search?q=lipopolysaccharide" title=" lipopolysaccharide"> lipopolysaccharide</a>, <a href="https://publications.waset.org/abstracts/search?q=apoptosis" title=" apoptosis"> apoptosis</a> </p> <a href="https://publications.waset.org/abstracts/198176/apoptosis-and-alterations-in-p21-and-p27-levels-in-human-primary-aniridia-limbal-stromal-cells-in-an-lps-induced-inflammatory-microenvironment-in-vitro" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/198176.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">12</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2</span> Effect of Travoprost on Cell Viability, Proliferation and Migration in the Sirna-Based Aniridia Limbal Epithelial Cell Model and in Primary Aniridia Limbal Stromal Cells, in Vitro</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Shuailin%20Li">Shuailin Li</a>, <a href="https://publications.waset.org/abstracts/search?q=Tanja%20Stachon"> Tanja Stachon</a>, <a href="https://publications.waset.org/abstracts/search?q=Fabian%20N.%20Fries"> Fabian N. Fries</a>, <a href="https://publications.waset.org/abstracts/search?q=Zhen%20Li"> Zhen Li</a>, <a href="https://publications.waset.org/abstracts/search?q=Shanhe%20Liu"> Shanhe Liu</a>, <a href="https://publications.waset.org/abstracts/search?q=Shao-Lun%20Hsu"> Shao-Lun Hsu</a>, <a href="https://publications.waset.org/abstracts/search?q=Berthold%20Seitz"> Berthold Seitz</a>, <a href="https://publications.waset.org/abstracts/search?q=Swarnali%20Kundu"> Swarnali Kundu</a>, <a href="https://publications.waset.org/abstracts/search?q=Maryam%20Amini"> Maryam Amini</a>, <a href="https://publications.waset.org/abstracts/search?q=Shweta%20Suiwal"> Shweta Suiwal</a>, <a href="https://publications.waset.org/abstracts/search?q=N%C3%B3ra%20Szentm%C3%A1ry"> Nóra Szentmáry</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Purpose: Aniridia associated keratopathy (AAK) is a progressive condition commonly observed in individuals with congenital aniridia, with PAX6 haploinsufficiency. AAK can lead to limbal stem cell deficiency and progressive ocular surface damage. The dysfunction of limbal epithelial and stromal cells (LECs and LSCs) potentially plays a key role in AAK pathogenesis. Travoprost, a prostaglandin analog, has been shown to influence cellular behavior in various cell types, but its effects on primary aniridia LECs and LSCs remain unclear. This study aims to evaluate the impact of travoprost on cell viability, proliferation, and migration in LECs, in the siRNA-based limbal epithelial cell model and in primary aniridia LSCs, in vitro. Methods: Primary human LECs were extracted from heathy donors, and siRNA treatment was used to mimic PAX6 haploinsufficiency in congenital aniridia. Primary human LSCs were extracted from heathy and aniridia donors (AN-LSCs). LECs, LSCs and AN-LSCs were treated with 0.039-40 μg/ml travoprost, for 20 minutes. The XTT and BrdU assays were used to evaluate the effect of travoprost on cell viability and proliferation (n=7). Cell migration assay was performed following siRNA-based PAX6 knockdown and subsequent 0.313 and 0.156 μg/ml travoprost treatment for 20 minutes (n=5). Results: LECs and LSCs viability decreased significantly from 0.156 μg/ml and ANLSCs viability decreased significantly from 0.078 μg/ml travoprost concentration (p=0.0279, p<0.0001, p=0.0002). In all cell types, there was a stepwise decrease in cell viability, as the travoprost concentration increased (p<0.0001). Travoprost treatment did not change cell proliferation in LSCs (p≥0.0892). In LECs, 20 and 40 μg/ml travoprost concentration, in AN-LSCs 40 μg/ml travoprost concentration exhibited reduced cell proliferation, compared to untreated controls (p=0.0291, p=0.0041, p=0.0011). PAX6-knockdown LECs exhibited lower migration rates at 6, 12, and 24 hours (p=0.0015, p=0.0471, p=0.0009) than control siRNA treated LECs, following travoprost treatment. In contrast, AN-LSCs demonstrated higher migration rates at the same 3 time points, than LSCs, after treatment (p=0.0225, p=0.0383, p=0.0155). In addition, among AN-LSCs, migration rate at of the 0.313 μg/ml travoprost treated group at 6 hours was significantly higher, than in the untreated control group. Conclusions: Our results demonstrate that travoprost may exert different effects on LECs, PAX6-knockdown LECs, LSCs, and AN-LSCs regarding cell viability, proliferation, and migration. AN-LSCs appear to exhibit greater sensitivity to travoprost treatment, than LSCs, therefore, topical antiglaucomatous treatment should be selected with caution for patients with congenital aniridia. Further in vivo measurements are necessary to evaluate the potential role of travoprost on AAK. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=congenital%20aniridia" title="congenital aniridia">congenital aniridia</a>, <a href="https://publications.waset.org/abstracts/search?q=travoprost" title=" travoprost"> travoprost</a>, <a href="https://publications.waset.org/abstracts/search?q=primary%20limbal%20epithelial%20cells" title=" primary limbal epithelial cells"> primary limbal epithelial cells</a>, <a href="https://publications.waset.org/abstracts/search?q=primary%20limbal%20stromal%20cells" title=" primary limbal stromal cells"> primary limbal stromal cells</a> </p> <a href="https://publications.waset.org/abstracts/198133/effect-of-travoprost-on-cell-viability-proliferation-and-migration-in-the-sirna-based-aniridia-limbal-epithelial-cell-model-and-in-primary-aniridia-limbal-stromal-cells-in-vitro" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/198133.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">11</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1</span> Effect of Retinoic Acid Treatment on the Retinoic Acid Signaling Pathway in a siRNA-Based Aniridia Limbal Epithelial Cell Model, in Vitro</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Shao-Lun%20Hsu">Shao-Lun Hsu</a>, <a href="https://publications.waset.org/abstracts/search?q=Tanja%20Stachon"> Tanja Stachon</a>, <a href="https://publications.waset.org/abstracts/search?q=Fabian%20N.%20Fries"> Fabian N. Fries</a>, <a href="https://publications.waset.org/abstracts/search?q=Zhen%20Li"> Zhen Li</a>, <a href="https://publications.waset.org/abstracts/search?q=Shuailin%20Li"> Shuailin Li</a>, <a href="https://publications.waset.org/abstracts/search?q=Shanhe%20Liu"> Shanhe Liu</a>, <a href="https://publications.waset.org/abstracts/search?q=Berthold%20Seitz"> Berthold Seitz</a>, <a href="https://publications.waset.org/abstracts/search?q=Swarnali%20Kundu"> Swarnali Kundu</a>, <a href="https://publications.waset.org/abstracts/search?q=Maryam%20Amini"> Maryam Amini</a>, <a href="https://publications.waset.org/abstracts/search?q=Shweta%20Suiwal"> Shweta Suiwal</a>, <a href="https://publications.waset.org/abstracts/search?q=N%C3%B3ra%20Szentm%C3%A1ry"> Nóra Szentmáry</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Purpose: Congenital aniridia is characterized by PAX6 haploinsufficiency, and aniridia-associated keratopathy (AAK). In AAK, limbal stem cell deficiency and impaired wound healing are widely observed in patients, which might be associated with an imbalanced retinoic acid (RA) signaling pathway. In the previous studies, we demonstrated the relationship between PAX6 and the altered expression levels of key markers in the RA signaling pathway to retinol treatment. The present study evaluates the gene and protein expression levels in an in vitro small interfering RNA (siRNA) PAX6 knockdown aniridia limbal epithelial cell model, following retinoic acid treatment. This study targets the direct effects of active RA products and their association with key regulators of the RA signaling pathway in siRNA PAX6 knockdown LECs. Methods: Primary human limbal epithelial cells (LECs) were knocked down by siRNA treatment to mimic PAX6 deletion in congenital aniridia (n=8). This was followed by 0µM, 1µM, and 5µM retinoic acid treatment applied in both siRNA PAX6 control and knockdown groups. After 48h incubation, paired box 6 (PAX6), alcohol dehydrogenase 7 (ADH7), aldehyde dehydrogenase 1 family member A1 (ALDH1A1), cytochrome P450 family 26 subfamilies A member 1 (CYP26A1), retinol-binding protein 1 (RBP1), cellular retinoic acid binding protein 2 (CRABP2), fatty acid binding protein 5 (FABP5), retinoid X receptor alpha (RXRA), retinoid X receptor beta (RXRB), retinoic acid receptor alpha (RARA), retinoic acid receptor beta (RARB), peroxisome proliferator-activated receptor gamma (PPARG), vascular endothelial growth factor A (VEGFA) mRNA levels have been determined using qPCR and protein levels by ELISA or western blot. Results: PAX6, ADH7, ALDH1A1, FABP5 mRNA levels and PAX6, ADH7, FABP5, PPARG2 protein levels were significantly lower in the PAX6 knockdown group, than in controls (p<0.001, p=0.018, p=0.015, p<0.001; p<0.001, p=0.003, p<0.001, p=0.007). PPARG mRNA level was significantly higher in the PAX6 knockdown group than in controls (p=0.012). CYP26A1 mRNA expression was upregulated using 1 µM and 5 µM RA treatment in both the PAX6 control (p<0.001; p<0.001) and the PAX6 knockdown group (p=0.001; p=0.002). CRABP2 mRNA expression in the PAX6 knockdown group (p=0.02) and protein expression in both groups were downregulated using to 5 µM RA concentration (p=0.003; p=0.02). RARA mRNA expression in the PAX6 knockdown group (p=0.023), RARB mRNA expression in both groups (p=0.006, p=0.001), and RXRA protein expression in controls (p=0.007), were downregulated using to 5 µM RA concentration. VEGFA mRNA expression in PAX6 controls was upregulated using 5 µM RA (p=0.041). FABP5 to CRAP2 ratio was higher in PAX6 controls, than in the PAX6 knockdown group (p<0.001). Additionally, the FABP5 to CRAP2 ratio was only upregulated in PAX6 controls using 5 µM RA concentration but not in the PAX6 knockdown group (p<0.001). Conclusions: the results reveal a less-responsive FABP5 to CRABP2 ratio in PAX6 knockdown LECs following increased RA concentration, as well as altered expression of key regulators in the RA signaling pathway. Further investigation, expanding the sample size, mimicking different PAX6 mutations and exploring additional molecular mechanism impacted by RA treatment should offer potential treatment options in AAK, in the future. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=congenital%20aniridia" title="congenital aniridia">congenital aniridia</a>, <a href="https://publications.waset.org/abstracts/search?q=paired%20box%206%20gene" title=" paired box 6 gene"> paired box 6 gene</a>, <a href="https://publications.waset.org/abstracts/search?q=aniridia-associated%20keratopathy" title=" aniridia-associated keratopathy"> aniridia-associated keratopathy</a>, <a href="https://publications.waset.org/abstracts/search?q=retinoic%20acid%20signaling%20pathway" title=" retinoic acid signaling pathway"> retinoic acid signaling pathway</a> </p> <a href="https://publications.waset.org/abstracts/197382/effect-of-retinoic-acid-treatment-on-the-retinoic-acid-signaling-pathway-in-a-sirna-based-aniridia-limbal-epithelial-cell-model-in-vitro" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/197382.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">12</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates its 16th 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