CINXE.COM
miRNAscope HD Assay Red | In Situ Hybridization, RNA-ISH | ACDBio
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Assay Red</h3></div><div class="loader-image-gallery"></div><div id="film_roll" class="sample-slider"><div><img typeof="foaf:Image" src="https://acdbio.com/sites/default/files/styles/sample_image/public/img3.jpg" width="765" height="451" alt="miRNAscope™ assay used to detect miR-214-3p in different species samples including the mouse brain, rat brain, human brain, and mouse liver. miR-214-3p was successfully detected in the mouse, rat, and human brain tissue samples and not in the negative control mouse liver tissue sample. " title="miRNAscope™ assay used to detect miR-214-3p in different species samples including the mouse brain, rat brain, human brain, and mouse liver. miR-214-3p was successfully detected in the mouse, rat, and human brain tissue samples and not in the negative control mouse liver tissue sample. " /><p class="slider-below-text"> miRNAscope™ assay used to detect miR-214-3p in different species samples including the mouse brain, rat brain, human brain, and mouse liver. miR-214-3p was successfully detected in the mouse, rat, and human brain tissue samples and not in the negative control mouse liver tissue sample.</p></div><div><img typeof="foaf:Image" src="https://acdbio.com/sites/default/files/styles/sample_image/public/img2.jpg" width="765" height="451" alt="miRNAscope™ assay used to specifically detect miR-138-5p in Purkinje cells in cerebellum and hippocampus regions of the mouse brain. " title="miRNAscope™ assay used to specifically detect miR-138-5p in Purkinje cells in cerebellum and hippocampus regions of the mouse brain. " /><p class="slider-below-text"> miRNAscope™ assay used to specifically detect miR-138-5p in Purkinje cells in cerebellum and hippocampus regions of the mouse brain.</p></div><div><img typeof="foaf:Image" src="https://acdbio.com/sites/default/files/styles/sample_image/public/img1.jpg" width="765" height="451" alt="miRNAscope™ HD assay used to detect the outer nuclear layer (ONL) specific microRNA-182 in FFPE mouse eye sample. Negligible microRNA-182 is also detected in the inner nuclear layer and the ganglion cell layer." title="miRNAscope™ HD assay used to detect the outer nuclear layer (ONL) specific microRNA-182 in FFPE mouse eye sample. Negligible microRNA-182 is also detected in the inner nuclear layer and the ganglion cell layer." /><p class="slider-below-text"> miRNAscope™ HD assay used to detect the outer nuclear layer (ONL) specific microRNA-182 in FFPE mouse eye sample. Negligible microRNA-182 is also detected in the inner nuclear layer and the ganglion cell layer.</p></div></div><style type="text/css"> .loader-image-gallery { position: absolute; left: 0px; top: 0px; width: 100%; height: 100%; z-index: 9999; background: url('/sites/default/files/loading_apple.gif') 50% 35% no-repeat rgb(249,249,249); background-size: 100px; opacity: .8; } .sample-image { position: relative; } </style> <script type="text/javascript"> jQuery(document).ready(function($) { // $('#film_roll').hide(); function preloader(){ $('.loader-image-gallery').hide(); $('#film_roll').show(); } window.onload = preloader; }); </script> <div class="profile-content"><div class="field field-name-body field-type-text-with-summary field-label-hidden"><div class="field-items"><div class="field-item even" property="content:encoded"><p>Introducing the highly specific and sensitive miRNAscope™ Assay to detect small RNAs with spatial and morphological context at single-cell resolution. Leveraging ACD’s RNAscope™ core technology, the miRNAscope assay is designed to enable applications for small RNAs including the detection of antisense oligonucleotides (ASOs), microRNAs (miRNAs), small interfering RNAs (siRNAs), and other smaller RNAs that are 17-50 bases.</p><p>• Detect and identify cellular subtypes</p><p>• Visualize gene regulation with morphological context</p><p>• Validate miRNA biomarkers in intact tissues</p><p>• Assess small RNA therapeutic delivery mechanism</p><p>• Evaluate biodistribution and efficacy of therapy</p><p>• Add a visual dimension to heterogeneous tissue biology and analysis</p><p> </p><p>The miRNAscope™ HD Assay Workflow Requires the following:</p><ul><li>miRNAscope™ Target Probes (Catalog or Made-to-Order S1 Probes, not compatible with the RNAscope™ or BaseScope™ Assays)</li><li>miRNAscope™ Positive Control Probe (Cat. No. 727871-S1)</li><li>miRNAscope™ Negative Control Probe (Cat. No. 727881-S1)</li><li>miRNAscope™ HD Reagent Kit - RED (Cat. No. 324500) (Includes Pretreatment Kit, Detection kit, and wash buffer)</li><li>ImmEdge Hydrophobic Barrier Pen (Cat. No. 310018)</li><li>HybEZ™ Hybridization System</li></ul><div> </div><p>If using miRNAscope™ HD Assay for the first time, our Intro Packs include the universal positive and negative control probes, the Reagent Kit, the control cell pellets (Human or Mouse), the ImmEdge hydrophobic barrier pen, and the Vectamount mounting media. Be sure to order your specific miRNAscope™ target probes separately.</p><ul><li>miRNAscope™ Intro Pack HD Reagent Kit RED – Hsa (Cat. No. 324530)</li><li>miRNAscope™ Intro Pack HD Reagent Kit RED – Mmu (Cat. 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rgba(0,0,0,0.28); box-shadow: 0 7px 12px rgba(0,0,0,0.28); } @media only screen and (min-width: 48em) { /*mobile*/ .embeddedServiceInvitation { max-width: 332px; max-height: 210px; } } .embeddedServiceInvitation > .embeddedServiceInvitationHeader { width: inherit; height: 32px; line-height: 32px; padding: 10px; color: #FFFFFF; background-color: #222222; overflow: initial; display: flex; justify-content: space-between; align-items: stretch; border-top-left-radius: 8px; border-top-right-radius: 8px; } .embeddedServiceInvitationHeader #embeddedServiceAvatar { width: 32px; height: 32px; border-radius: 50%; } .embeddedServiceInvitationHeader .embeddedServiceTitleText { font-size: 18px; color: #FFFFFF; overflow: hidden; word-wrap: normal; white-space: nowrap; text-overflow: ellipsis; align-self: stretch; flex-grow: 1; max-width: 100%; margin: 0 12px; } .embeddedServiceInvitationHeader .embeddedServiceCloseIcon { border: none; border-radius: 3px; cursor: pointer; position: relative; bottom: 3%; background-color: transparent; width: 32px; height: 32px; font-size: 23px; color: #FFFFFF; } .embeddedServiceInvitationHeader .embeddedServiceCloseIcon:focus { outline: none; } .embeddedServiceInvitationHeader .embeddedServiceCloseIcon:focus::before { content: " "; position: absolute; top: 11%; left: 7%; width: 85%; height: 85%; background-color: rgba(255, 255, 255, 0.2); border-radius: 4px; pointer-events: none; } .embeddedServiceInvitationHeader .embeddedServiceCloseIcon:active, .embeddedServiceCloseIcon:hover { background-color: #FFFFFF; color: rgba(0,0,0,0.7); opacity: 0.7; } .embeddedServiceInvitation > .embeddedServiceInvitationBody { background-color: #FFFFFF; max-height: 110px; min-width: 260px; margin: 0 8px; font-size: 14px; line-height: 20px; overflow: auto; } .embeddedServiceInvitationBody p { color: #333333; padding: 8px; margin: 12px 0; } .embeddedServiceInvitation > .embeddedServiceInvitationFooter { width: inherit; color: #FFFFFF; text-align: right; background-color: #FFFFFF; padding: 10px; max-height: 50px; border-bottom-left-radius: 8px; border-bottom-right-radius: 8px; } .embeddedServiceInvitationFooter > .embeddedServiceActionButton { font-size: 14px; max-height: 40px; border: none; border-radius: 4px; padding: 10px; margin: 4px; text-align: center; text-decoration: none; display: inline-block; cursor: pointer; } .embeddedServiceInvitationFooter > #acceptInvite { background-color: #005290; color: #FFFFFF; } .embeddedServiceInvitationFooter > #rejectInvite { background-color: #FFFFFF; color: #005290; } </style> <!-- doFind, doCreate and isExactMatch example for a Contact: Find a contact whose Email exactly matches the value provided by the customer in the form If there's no match, then create a Contact record and set it's First Name, Last Name, Email, and Phone to the values provided by the customer --> <!-- <input type="hidden" name="liveagent.prechat.findorcreate.map.doFind:Contact" value="Email,true" /> <input type="hidden" name="liveagent.prechat.findorcreate.map.isExactMatch:Contact" value="Email,true" /> <input type="hidden" name="liveagent.prechat.findorcreate.map.doCreate:Contact" value="LastName,true;FirstName,true;Email,true" />--> <input type="hidden" name="liveagent.prechat.findorcreate.map.doFind:Contact" value="LastName,true;FirstName,true;Email,true" /> <input type="hidden" name="liveagent.prechat.findorcreate.map.isExactMatch:Contact" value="LastName,true;FirstName,true;Email,true" /> <input type="hidden" name="liveagent.prechat.findorcreate.map.doCreate:Contact" value="LastName,true;FirstName,true;Email,true" /> <script type='text/javascript'> (function() { document.getElementById('closeInvite').onclick = function() { embedded_svc.inviteAPI.inviteButton.rejectInvite(); }; document.getElementById('rejectInvite').onclick = function() { embedded_svc.inviteAPI.inviteButton.rejectInvite(); }; // use this API call to reject invitations document.getElementById('acceptInvite').onclick = function() { embedded_svc.liveAgentAPI.startChat({ directToButtonRouting: { buttonId: "5733t000000cVk9" }, extraPrechatInfo: [], extraPrechatFormDetails: [] }); }; // use this API call to start chat from invitations document.addEventListener('keyup', function(event) { if (event.keyCode == 27) { embedded_svc.inviteAPI.inviteButton.rejectInvite(); }}) })(); </script> <!-- End of Invitations --> <script type='text/javascript' src='https://service.force.com/embeddedservice/5.0/esw.min.js'></script> <script type='text/javascript'> var initESW = function(gslbBaseURL) { embedded_svc.settings.displayHelpButton = true; //Or false embedded_svc.settings.language = ''; //For example, enter 'en' or 'en-US' embedded_svc.settings.defaultMinimizedText = 'Chat now'; //(Defaults to Chat with an Expert) embedded_svc.settings.disabledMinimizedText = 'Leave a message'; //(Defaults to Agent Offline) embedded_svc.settings.autoOpenPostChat = true; //embedded_svc.settings.loadingText = ''; //(Defaults to Loading) //embedded_svc.settings.storageDomain = 'yourdomain.com'; //(Sets the domain for your deployment so that visitors can navigate subdomains during a chat session) // Settings for Chat embedded_svc.settings.directToButtonRouting = function (prechatFormData) { if (prechatFormData[4].value === "Technical Service") return "5733t000000XZAM"; if (prechatFormData[4].value === "Customer Care") return "5733t000000XZAH"; }; embedded_svc.settings.prepopulatedPrechatFields = { OmniURL__c: window.location.href, ChooseaDepartment__c: 'Technical Service' }; //Sets the auto-population of pre-chat form fields embedded_svc.settings.extraPrechatFormDetails = [{ "label":"Source Domain", "value":window.location.href, "transcriptFields":[ "Source_Domain__c" ], "displayToAgent":true }]; //embedded_svc.settings.fallbackRouting = []; //An array of button IDs, user IDs, or userId_buttonId embedded_svc.settings.offlineSupportMinimizedText = 'Leave a message'; //(Defaults to Contact Us) embedded_svc.settings.enabledFeatures = ['LiveAgent']; embedded_svc.settings.entryFeature = 'LiveAgent'; embedded_svc.init( 'https://biotechne.my.salesforce.com', 'https://biotechne.my.salesforce-sites.com/LiveAgent', gslbBaseURL, '00D500000006gkl', 'ACD_Chat_Automated', { baseLiveAgentContentURL: 'https://c.la1-c1-ia5.salesforceliveagent.com/content', deploymentId: '572380000004CqL', buttonId: '5733t000000cVk9', baseLiveAgentURL: 'https://d.la1-c1-ia5.salesforceliveagent.com/chat', eswLiveAgentDevName: 'EmbeddedServiceLiveAgent_Parent04I3t000000Y7zbEAC_175bb741b99', isOfflineSupportEnabled: true } ); }; if (!window.embedded_svc) { var s = document.createElement('script'); s.setAttribute('src', 'https://biotechne.my.salesforce.com/embeddedservice/5.0/esw.min.js'); s.onload = function() { initESW(null); }; document.body.appendChild(s); } else { initESW('https://service.force.com'); } </script> <script src="//rum-static.pingdom.net/pa-5c133f6e1872b50016000024.js" async></script> <script type="text/javascript">window.NREUM||(NREUM={});NREUM.info={"beacon":"bam.nr-data.net","licenseKey":"NRJS-dd6144c4de227d09eb4","applicationID":"1022125825","transactionName":"YFxbZkpUXBYEVkYNWVkWbEBRGlsLAVBKSkZfSQ==","queueTime":0,"applicationTime":284,"atts":"TBtYEAJOTxg=","errorBeacon":"bam.nr-data.net","agent":""}</script></body> </html>