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定制的微流控芯片设计实现具有广视野的低成本三维时空转录组学研究—小柯机器人—科学网
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However, integrating cost-effectiveness, high throughput, a wide field of view and compatibility with three-dimensional (3D) volumes has been challenging. Here we introduce microfluidics-assisted grid chips for spatial transcriptome sequencing (MAGIC-seq), a new method that combines carbodiimide chemistry, spatial combinatorial indexing and innovative microfluidics design. This technique allows sensitive and reproducible profiling of diverse tissue types, achieving an eightfold increase in throughput, minimal cost and reduced batch effects. MAGIC-seq breaks conventional microfluidics limits by enhancing barcoding efficiency and enables analysis of whole postnatal mouse sections, providing comprehensive cellular structure elucidation at near single-cell resolution, uncovering transcriptional variations and dynamic trajectories of mouse organogenesis. Our 3D transcriptomic atlas of the developing mouse brain, consisting of 93 sections, reveals the molecular and cellular landscape, serving as a valuable resource for neuroscience and developmental biology. Overall, MAGIC-seq is a high-throughput, cost-effective, large field of view and versatile method for spatial transcriptomic studies.</p> <p> DOI: 10.1038/s41588-024-01906-4</p> <p> Source: <a href="https://www.nature.com/articles/s41588-024-01906-4">https://www.nature.com/articles/s41588-024-01906-4</a></p> </p> <div class="remarks">期刊信息</div> <div class="dd"><p> <span style="font-weight: bold;">Nature Genetics：</span>《自然—遗传学》，创刊于1992年。隶属于施普林格·自然出版集团，最新IF：41.307<br /> <span style="font-weight: bold;">官方网址：</span><a href="https://www.nature.com/ng/" target="_blank">https://www.nature.com/ng/</a><br /> <span style="font-weight: bold;">投稿链接：</span><a href="https://mts-ng.nature.com/cgi-bin/main.plex" target="_blank">https://mts-ng.nature.com/cgi-bin/main.plex</a></p> </div> </div> </div> <style> #footer{ background:#890f0e; text-align:center; padding:10px; color:#FFF; font-size:12px;} #footer a{color:#FFF; font-size:12px;} </style> <div id="footer"><a href="http://www.sciencenet.cn/aboutus/" target="_blank">关于我们</a> | <a href="http://www.sciencenet.cn/shengming.aspx" target="_blank">网站声明</a> | <a href="http://www.sciencenet.cn/tiaokuang.aspx" target="_blank">服务条款</a> | <a href="http://www.sciencenet.cn/contact.aspx" target="_blank">联系方式</a> | <a href="http://www.sciencenet.cn/RSS.aspx">RSS</a> | <a href="mailto:jubao@stimes.cn">举报</a> | <a href="https://stimes.sciencenet.cn/">中国科学报社</a> <br> <a href="https://beian.miit.gov.cn" target="_blank" > 京ICP备07017567号-12 </a> 互联网新闻信息服务许可证10120230008 京公网安备 11010802032783<br>Copyright @ 2007-<script type="text/javascript">var Date22 = new Date();var year22 = Date22.getFullYear();document.write(year22);</script> 中国科学报社 All Rights Reserved<br> 地址：北京市海淀区中关村南一条乙三号 邮箱：blog@stimes.cn <br> <span style="display:none"> <script type="text/javascript"> var _bdhmProtocol = (("https:" == document.location.protocol) ? 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