Cryopreserved Human PBMCs

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hundreds of aliquots from each subject. You therefore may repeat and expand experiments using the same cells, “indefinitely”. These ePBMC have been characterized with respect to their T- and B-cell reactivity to SARS-CoV-2, HCMV, EBV, influenza, and other common recall antigens. Such cells are indispensable for antigen-specific T and B cell ELISPOT/FluoroSpot assay development and validation, as well as proficiency testing, with all of which CTL can assist. Entire studies can be conducted with these cells. Need a cohort of HLA-allele-matched donors? Interested in any other allele? We have dozens of subjects for most alleles at your fingertip, no IRB needed!</h5> <a class="btn btn-primary" href="http://epbmc.immunospot.com" target="_blank" rel="noreferrer noopener" role="button">Search Database</a> <div class="bg-size-cover bg-position-center shadow-lg bg-no-repeat text-center py-7 mb-0 mt-4 rounded" style="width: 500px; background-image: url('/pub/media/images/epbmc/epbmc-main-video.jpg');"> <a class="my-2 video-caption" href="#" data-bs-toggle="modal" data-bs-target="#video-main" data-sub-html='<h6 class="fs-sm text-light">Video caption</h6>'><i class="ai-play fs-1 text-light mt-1 me-2 pe-1"></i></a> <br> <br> <span class="text-light fs-6 p-2 fw-bolder">PBMC, Cryopreserved Peripheral Blood Mononuclear Cells</span> </div> </div> <div class="col-md-3 offset-1"> <div class="widget widget-categories shadow rounded px-3 py-2"> <ul> <li><a class="widget-link text-primary" href="/products/epbmc/full-exome-characterized.html" target="_blank" rel="noopener">Full Genome</a></li> <li><a class="widget-link text-primary" href="/products/epbmc/hla-typed-characterized-pbmc.html" target="_blank" rel="noopener">HLA-typed</a></li> <li><a class="widget-link text-primary" href="/products/epbmc/fcgriiia-polymorphism-characterized-pbmc.html" target="_blank" rel="noopener">FcγRIIIa Polymorphism</a></li> <li><a class="widget-link text-primary" href="/products/epbmc/t-cell-reactivity-characterized-pbmc.html" target="_blank" rel="noopener">T Cell Reactivity</a></li> <li><a class="widget-link text-primary" href="/products/epbmc/b-cell-reactivity-characterized.html" target="_blank" rel="noopener">B Cell Reactivity</a></li> <li><a class="widget-link text-primary" href="/products/epbmc/nk-activity-pbmc.html" target="_blank" rel="noopener">NK-Activity</a></li> <li><a class="widget-link text-primary" href="/products/epbmc/reference-samples-qc-set.html" target="_blank" rel="noopener">Positive/Negative Control PBMC</a></li> <li><a class="widget-link text-primary" href="/epbmc-sars-1.html" target="_blank" rel="noopener">SARS-CoV-2 characterized PBMC</a></li> <li><a class="widget-link text-primary" href="/products/epbmc/pooled-mat-pbmc.html" target="_blank" rel="noopener">Pooled PBMC</a></li> <li><a class="widget-link text-primary" href="/products/epbmc/uncharacterized-pbmc.html" target="_blank" rel="noopener">Uncharacterized PBMC</a></li> <li><a class="widget-link text-primary" href="/products/epbmc/sampling-sets-pbmc.html" target="_blank" rel="noopener">PBMC Sampling Sets</a></li> </ul> </div> </div> </div> </div> <div class="container epbmc-carousel my-5"> <ul class="nav nav-tabs border-bottom mb-0 justify-content-center justify-content-lg-start" role="tablist"> <li class="nav-item my-3 position-relative"><a class="nav-link mr-2 px-3 mr-2 active" role="tab" href="#applications" data-bs-toggle="tab">Applications</a></li> <li class="nav-item my-3 position-relative"><a class="nav-link mr-2 px-3 mr-2" role="tab" href="#characterization" data-bs-toggle="tab">Characterization</a></li> <li class="nav-item my-3 position-relative"><a class="nav-link mr-2 px-3 mr-2" role="tab" href="#convenience" data-bs-toggle="tab">Convenience</a></li> <li class="nav-item my-3 position-relative"><a class="nav-link mr-2 px-3 mr-2" role="tab" href="#resources" data-bs-toggle="tab">Additional Resources</a></li> <li class="nav-item my-3 position-relative"><a class="nav-link mr-2 px-3 mr-2" role="tab" href="#availability" data-bs-toggle="tab">Instant Worldwide Availability</a></li> <li class="nav-item my-3 position-relative"><a class="nav-link mr-2 px-3 mr-2" role="tab" href="#videos" data-bs-toggle="tab">ePBMC Videos</a></li> </ul> <div class="tab-content"> <div id="applications" class="tab-pane fade show active"> <div class="row bg-grey p-3 text-sm-left"> <div class="col-sm-7 mb-3 mb-sm-0"> <h3 class="h5 mb-4 font-weight-bold">Applications</h3> <p>Individual healthy donor cells are available in batches of up to 400 to 800 vials for any application that requires live, functional PBMC.</p> <table class="slider-text" style="width: 100%;"> <tbody> <tr> <td>ELISPOT</td> <td>Cytokine Protein Arrays (CPA)</td> </tr> <tr> <td>NK assays</td> <td>Cytokine mRNA Determinations (RT-PCR)</td> </tr> <tr> <td>Tetramers/Pentamers</td> <td>ADCC</td> </tr> <tr> <td>Intracytoplasmic Cytokine Staining (ICS)</td> <td>Transmigration Assay</td> </tr> <tr> <td>Cytokine ELISA Assays</td> <td>Cytotoxicity Assays</td> </tr> <tr> <td>Cytokine Bead Arrays (CBA)</td> <td>Toxicology studies</td> </tr> </tbody> </table> </div> <div class="col-sm-5"> <img class="rounded" src="https://immunospot.com/media/images/epbmc/carousel-applications.jpg" alt="" /> </div> </div> </div> <div id="characterization" class="tab-pane fade"> <div class="row bg-grey p-3 text-sm-left"> <div class="col-sm-7 mb-3 mb-sm-0"> <h3 class="h5 mb-4 font-weight-bold">Characterization</h3> <p>Immune characterized PBMC are available in large batches of individual cryopreserved vials with predetermined frequencies of antigen-specific IFN-g, IL-2, IL-4, IL-5, IL-17 secreting cells.</p> <p>Each batch of PBMC has been tested for reactivity in ELISPOT assays for Class I-restricted antigens: 32 Class I individual CEF-peptides and 2 Class I CEF-Peptide Pools and Class II antigens: 24 Class II individual CEF peptides, 1 Class II CEF-Peptide Pool, and 6 Protein antigens (Mosquito, Dust Mite, Mumps, Candida, PPD, PHA), as well as our new CPI antigen peptide pool, which was developed as a CD4+ specific positive control.</p> <p>High resolution HLA-typed for three Class I loci (A, B, C) and four Class II loci (DRB1, DQA1, DQB1, and DP)</p> <p>Fully characterized donor demographics, vaccination status and antigen reactivity.</p> <p>Reference samples for designing your own experiments or for screening the reactivity of uncharacterized PBMC against your specific antigen or compound of interest.</p> </div> <div class="col-sm-5"><img class="img-fluid rounded" src="https://immunospot.com/media/images/epbmc/carousel-characterization.jpg" alt=""></div> </div> </div> <div id="convenience" class="tab-pane fade"> <div class="row bg-grey p-3 text-sm-left"> <div class="col-sm-7 mb-3 mb-sm-0"> <h3 class="h5 mb-4 font-weight-bold">Convenience</h3> <p>ePBMC samples are isolated from leukocytes collected by leukapharesis and are frozen in CTL-Cryo™ ABC serum-free freezing medium.</p> <p>The leukapharesis samples are collected in full compliance with HIPAA guidelines to eliminate any potential breach of privacy. They are tested for: HBsAg, HBcAb, HCV, HTLV I/II and STS by serology; as well as HIV I, HCV and WNV by NAT (nucleic acid testing).</p> <p>No IRBs required – We’ve taken care of all ethical and regulatory compliance issues so that you can purchase donor samples matching your requirements with no hassle.</p> </div> <div class="col-sm-5"><img class="img-fluid rounded" src="https://immunospot.com/media/images/epbmc/carousel-convenience.jpg" alt=""></div> </div> </div> <div id="resources" class="tab-pane fade"> <div class="row bg-grey p-3 text-sm-left"> <div class="col-sm-7 mb-3 mb-sm-0"> <ul class="no-indent"> <li><a href="/pub/media/mageplaza/product_attachments/attachment_file/c/t/ctl-epbmc-brochure.pdf" target="_blank" rel="noopener">ePBMC® Brochure</a></li> <li><a href="/pub/media/mageplaza/product_attachments/attachment_file/c/t/ctl-serum-free-media-brochure.pdf" target="_blank" rel="noopener">CTL Serum-free Media Brochure</a></li> <li><a href="/pub/media/mageplaza/product_attachments/attachment_file/p/r/protocols-guidelines_for_pbmc_022717.pdf" target="_blank" rel="noopener">Protocols & Guidelines for Working with PBMC</a></li> <li><a href="/pub/media/mageplaza/product_attachments/attachment_file/r/e/resting-cryopreserved-pbmc.pdf" target="_blank" rel="noopener">Resting of cryopreserved PBMC does not generally benefit the performance of antigen-specific T cell ELISPOT Assays</a></li> <li><a href="/pub/media/mageplaza/product_attachments/attachment_file/t/h/thawing-cryopreserved-pbmc.pdf" target="_blank" rel="noopener">Optimal thawing of cryopreserved PBMC for use in high-throughput human immune monitoring studies</a></li> <li><a href="/pub/media/mageplaza/product_attachments/attachment_file/e/l/elispot-in-384-well-format_compressed.pdf" target="_blank" rel="noopener">ELISPOT assays in 384-well format allow analysis of 400 independent T cell tests with 10ml blood</a></li> <li><a href="/pub/media/mageplaza/product_attachments/attachment_file/e/l/elispot-has-reproducible-results-different-labs-inexperienced-investigators_compressed_1.pdf" target="_blank" rel="noopener">ELISPOT assays provide reproducible results among different laboratories for T cell immune monitoring even in the hands of inexperienced investigators</a></li> </ul> </div> <div class="col-sm-5"><img class="img-fluid rounded" src="https://immunospot.com/media/images/epbmc/carousel-brochure.jpg" alt=""></div> </div> </div> <div id="availability" class="tab-pane fade"> <div class="row bg-grey p-3 text-sm-left"> <div class="col-sm-7 mb-3 mb-sm-0"> <h3 class="h5 mb-4 font-weight-bold">Instant Worldwide Availability</h3> <p>Shipped overnight within the continental US.</p> <p>Shipments to Europe and Japan take approximately 3 days, with cells shipped in Nitrogen vapor at -180C.</p> <p>ePBMC® are shipped in a cryopreservation container to ensure that their functionality is unaffected by the shipping, no matter where you are. Guaranteed!</p> <p>For shipping outside of the US, please contact <a href="https://immunospot.com/contact-support/contact-us" target="_self" name="Distributors">the distributor in your area</a>, or<a href="http://www.immunospot.com/contact-form" name="Contact us"> contact us</a> directly.</p> </div> <div class="col-sm-5"><img class="img-fluid rounded" src="https://immunospot.com/media/images/epbmc/carousel-world.jpg" alt=""></div> </div> </div> <div id="videos" class="tab-pane fade"> <div class="row bg-grey p-3 text-sm-left"> <div class="col-sm-6 mb-3 mb-sm-0"> <div class="row no-gutters mb-1"> <div class="col-md-12"> <div class="video-still lg bg-size-cover bg-position-center bg-no-repeat text-center py-5 mb-0" style="background-image: url('/pub/media/images/epbmc/video-lg.jpg');"><a class="cs-video-btn cs-video-btn-sm my-2" href="#" data-bs-toggle="modal" data-bs-target="#video-lg"> </a> <br> <div class="video-caption">PBMC, Cryopreserved Peripheral Blood Mononuclear Cells</div> </div> </div> </div> <div class="row no-gutters"> <div class="col-md-4 col-sm-3 pr-1"> <div class="video-still sm" style="background-image: url('/pub/media/images/epbmc/video-5.jpg');"><a class="video-caption" href="#" data-bs-toggle="modal" data-bs-target="#video-5">Ficoll Separation Method #1</a></div> </div> <div class="col-md-4 col-sm-3 pr-1"> <div class="video-still sm" style="background-image: url('/pub/media/images/epbmc/video-9.jpg');"><a class="video-caption" href="#" data-bs-toggle="modal" data-bs-target="#video-9">Ficoll Separation Method #2</a></div> </div> <div class="col-md-4 col-sm-3"> <div class="video-still sm" style="background-image: url('/pub/media/images/epbmc/video-2.jpg');"><a class="video-caption" href="#" data-bs-toggle="modal" data-bs-target="#video-2">Ficoll Separation Method #3</a></div> </div> </div> </div> <div class="col-sm-6"> <div class="row no-gutters mb-1"> <div class="col-md-4 col-sm-3 pr-1"> <div class="video-still sm" style="background-image: url('/pub/media/images/epbmc/video-1.jpg');"><a class="video-caption" href="#" data-bs-toggle="modal" data-bs-target="#video-1">Diluting Blood Before Isolating PBMC</a></div> </div> <div class="col-md-4 col-sm-3 pr-1"> <div class="video-still sm" style="background-image: url('/pub/media/images/epbmc/video-3.jpg');"><a class="video-caption" href="#" data-bs-toggle="modal" data-bs-target="#video-3">Resuspend PBMC Cell Pellet</a></div> </div> <div class="col-md-4 col-sm-3"> <div class="video-still sm" style="background-image: url('/pub/media/images/epbmc/video-4.jpg');"><a class="video-caption" href="#" data-bs-toggle="modal" data-bs-target="#video-4">Apply Adhesive Plate Sealer</a></div> </div> </div> <div class="row no-gutters mb-1"> <div class="col-md-4 col-sm-3 pr-1"> <div class="video-still sm" style="background-image: url('/pub/media/images/epbmc/video-6.jpg');"><a class="video-caption" href="#" data-bs-toggle="modal" data-bs-target="#video-6">Balance & Spin Vials for PBMC Isolation</a></div> </div> <div class="col-md-4 col-sm-3 pr-1"> <div class="video-still sm" style="background-image: url('/pub/media/images/epbmc/video-7.jpg');"><a class="video-caption" href="#" data-bs-toggle="modal" data-bs-target="#video-7">Storing the Cells</a></div> </div> <div class="col-md-4 col-sm-3"> <div class="video-still sm" style="background-image: url('/pub/media/images/epbmc/video-8.jpg');"><a class="video-caption" href="#" data-bs-toggle="modal" data-bs-target="#video-8">Create Humidified Chamber</a></div> </div> </div> <div class="row no-gutters"> <div class="col-md-4 col-sm-3 pr-1"> <div class="video-still sm" style="background-image: url('/pub/media/images/epbmc/video-10.jpg');"><a class="video-caption" href="#" data-bs-toggle="modal" data-bs-target="#video-10">Collect Mononuclear Cells</a></div> </div> <div class="col-md-4 col-sm-3 pr-1"> <div class="video-still sm" style="background-image: url('/pub/media/images/epbmc/video-11.jpg');"><a class="video-caption" 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