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checked item */ ul.inputs-list.multi-container li.hs-form-checkbox label span::after, .hs-form-booleancheckbox-display span::after { content: ""; border: 2px solid #d9d9d9; border-radius: 3px; height: 20px; left: 0px; position: absolute; top: 0px; width: 20px; background: #fff; z-index: 0; display: block; transition-property: background-color, border-color, -webkit-box-shadow; -o-transition-property: background-color, border-color, box-shadow; transition-property: background-color, border-color, box-shadow; transition-property: background-color, border-color, box-shadow, -webkit-box-shadow; } /* Show when checked */ ul.inputs-list.multi-container li.hs-form-checkbox label input:checked + span::after, .hs-form-booleancheckbox-display input:checked + span::after, ul.inputs-list.multi-container li.hs-form-checkbox label input:checked + span::before, .hs-form-booleancheckbox-display input:checked + span::before{ opacity: 1; } .hs-form-booleancheckbox-display input:checked+label:after, .hs-form-booleancheckbox-display input:checked+label:after { background: #fff; border-color: #157479; } /* Style when focused */ ul.inputs-list.multi-container li.hs-form-checkbox label input:focus + span::after, .hs-form-booleancheckbox-display input:focus + span::after { outline: 3px solid transparent; /* For Windows high contrast mode. */ box-shadow: 0 0 .3125rem rgba(0, 0, 0, .3); border-color: #157479; } .hs_contact_country{ clear: both; } .hs-button { border-radius: 65px !important; font-size: 14px; line-height: 1.3; letter-spacing: 1.12px; font-family: "Roboto Slab", helvetica, arial, sans-serif; font-weight: 700 !important; padding: 19px 23px !important; border: 2px solid; display: inline-block; text-decoration: none; text-transform: uppercase; -webkit-appearance: none !important; -moz-appearance: none !important; appearance: none !important; } .hbspt-form textarea{max-width: 100% !important; height: 186px !important; resize: none;} .hbspt-form textarea:focus{border: 1px solid #74cbc8 !important;} .hs-fieldtype-textarea .invalid.error{border: 2px solid #E74949 !important;} .hbspt-form fieldset{max-width: 100% !important; margin-bottom: 60px;} .hbspt-form .field {margin-bottom: 27px} .hs_recaptcha{ margin-top: 23px !important; margin-bottom: 27px !important; } .hs-button, .hs-form-field input[type=text], .hs-form-field input[type=email], .hs-form-field input[type=phone], .hs-form-field input[type=number], .hs-form-field input[type=tel], .hs-form-field input[type=date], .hs-form-field textarea { appearance: none; -webkit-appearance: none; -moz-appearance: none; } .hs-default-font-element, .hs-main-font-element { font-family: "Roboto Slab", helvetica, arial, sans-serif; } .hbspt-form .hs-button { white-space: pre-wrap } .hbspt-form .hs-richtext { word-break: break-word } .hbspt-form.submitted-message { overflow: auto } .hbspt-form fieldset.form-columns-1 .hs-input { width: 95% } .hbspt-form fieldset.form-columns-1 .input { margin-right: 0px !important } .hbspt-form fieldset.form-columns-1 input[type=checkbox], .hbspt-form fieldset.form-columns-1 input[type=radio] { width: auto } .hbspt-form fieldset.form-columns-2 .hs-form-field { width: 50%; float: left } .hbspt-form fieldset.form-columns-2 .input { margin-right: 0px !important; } .hbspt-form fieldset.form-columns-3 .hs-form-field { width: 32.7%; float: left } .hbspt-form fieldset.form-columns-3 .input { margin-right: 8px } .hbspt-form label.hs-hidden { visibility: hidden } .hbspt-form .hs-field-desc { width: 100% } .hs-custom-style .hs-input, .hs-custom-style fieldset { max-width: 100% } .hs-custom-style>div.form-columns-3 .hs-form-field, .hs-custom-style fieldset.form-columns-3 .hs-form-field { width: 33.3% } .hs-custom-style>div>div:last-of-type .hs-input:not([type=checkbox]):not([type=radio]), .hs-custom-style fieldset>div:last-of-type .hs-input:not([type=checkbox]):not([type=radio]) { width: 100%; max-width: 100% } .hs-custom-style>div input:not([type=image]):not([type=submit]):not([type=button]):not([type=radio]):not([type=checkbox]):not([type=file]), .hs-custom-style fieldset input:not([type=image]):not([type=submit]):not([type=button]):not([type=radio]):not([type=checkbox]):not([type=file]) { box-sizing: border-box; padding: 0 15px; min-height: 27px } .hs-custom-style>div textarea, .hs-custom-style fieldset textarea { padding: 10px 15px } .hs-custom-style .hs-dependent-field>div .hs-input:not([type=checkbox]):not([type=radio]) { width: 100% } .legal-consent-container .field.hs-form-field { margin-bottom: 8px } .legal-consent-container .hs-field-desc.checkbox-desc { margin: -12px 0 0 21px } .legal-consent-container .hs-form-booleancheckbox-display input { float: left } .legal-consent-container .hs-form-booleancheckbox-display>span { display: block !important; margin-left: 30px !important; } .legal-consent-container .hs-form-booleancheckbox-display p { margin: 0; display: inline } .legal-consent-container .hs-error-msgs label { color: #f2545b } .legal-consent-container~.hs_recaptcha { margin-top: 18px } .cookie-reset-container { font-size: 14px; margin-bottom: 10px; text-align: right } #hs-outer-captcha-target, #hs-outer-captcha-target * { display: none; height: 0; width: 0 } .hubspot-link__container { font-size: 14px; padding-bottom: 40px; position: relative; color: #9fa0a2; font-family: "Roboto Slab", helvetica, arial, sans-serif; } .hubspot-link-text { color: #00a4bd; font-weight: 400 } .hubspot-link__container.sproket { color: #9fa0a2 } .hubspot-link { color: #9fa0a2 } .hubspot-link, .hubspot-link:hover { text-decoration: none } .hubspot-link:hover .hubspot-link-text { text-decoration: underline } .hubspot-link__icon { margin-bottom: -1px; margin-right: 5px } .hubspot-link__container.sproket .hubspot-link__icon { width: 30px; margin-right: 0; float: left; margin-top: -9px; margin-left: -5px } .hubspot_signup_form .hs_email{margin-bottom: 0px !important; width: 80% !important; float: left !important;} .hubspot_signup_form .hs_submit{width: 20% !important; float: left !important; } .hubspot_signup_form .hs-button{padding: 12.5px 15px !important;} .hubspot_signup_form .hs_email label{display: none !important;} .hubspot_signup_form ul.no-list{position: absolute !important; top: 48px !important;} .hubspot_signup_form {position: relative !important;} .hubspot_signup_form .submitted-message{color: #ab8940 !important; font-size: 22px !important; } .hubspot_white .hbspt-form label, .hubspot_white .hs-form-required { color: #fff; } .hubspot_white .hs-input, .hubspot_white ul.inputs-list.multi-container li.hs-form-checkbox label span::before, .hubspot_white .hs-form-booleancheckbox-display span::before{ background-color: #fff !important; } .hubspot_white .hs_submit { text-align: center; } .hubspot_white .hs-form select.is-placeholder { opacity: 1; } .hubspot_white ul.inputs-list.multi-container li.hs-form-checkbox label span::after, .hubspot_white .hs-form-booleancheckbox-display span::after { border-color: #3f4852; } input[type=submit].hs-button.primary.large{ color: #fff; background: #00181e; border-color: #00181e !important; } input[type=submit].hs-button.primary.large:hover { background: #74cbc8 !important; border-color: #74cbc8 !important; } /***/ .hbspt-form .hs-input[type=checkbox]{ cursor: pointer; } .hs-form-booleancheckbox-display input:checked + span::after{ background: #fff; border-color: #157479; } .hbspt-form .form-columns-2 { display: flex; flex-flow: row wrap; -webkit-box-direction: normal; } .hbspt-form .hs_firstname { flex: 1; -webkit-box-flex: 1; } .hbspt-form .hs_submit{ margin-top: 60px; } .newsletter-form .hs_submit{margin-top: 45px;} .newsletter-form form .gfield_consent_description { border: none !important; } /*New Landing page*/ .landing-middle-section{ padding-right: 0px !important; } .left-col{max-width:93%} .left-col-para{padding-right:30px;} .left-col video{border-radius:4px;} /* Kit asssesment */ .kits_form_col .hbspt-form .hs-input[type=checkbox] { height: 20px !important; width: 20px !important; } .kits_form_col {padding-bottom: 45px;} .hs-richtext h4 {margin-bottom: 15px !important;} .kits_form_col label.hs-form-checkbox-display {margin-bottom: 15px;} .kits_form_col .hbspt-form li.hs-form-checkbox label span{ margin-left: 0px !important; padding-left: 31px; } .hbspt-form label.hs-form-radio-display { position: relative; padding: 4px 31px; } .hbspt-form .hs-form-radio-display span, .hbspt-form .hs-form-checkbox-display span { position: relative; cursor: pointer; font-size: 15px; font-weight: 300; } .hbspt-form .hs-input[type=radio] { position: absolute; left: 0; z-index: 2; width: 20px !important; height: 20px !important; outline: none; cursor: pointer; background-color: transparent !important; border-radius: 50%; } .hbspt-form .hs_protocol_synopsis_upload .input{position: relative;} .hbspt-form input[type="file"]::-webkit-file-upload-button { cursor: pointer; background-color: #157479; padding: 10px 20px !important; border: 1px solid #157479; border-top-left-radius: 0px; border-bottom-left-radius: 0px; border-top-right-radius: 6px; border-bottom-right-radius: 6px; color: #fff; position: absolute; top: 0; bottom: 0; right: 0; } .hbspt-form input[type="file"] { cursor: pointer; padding: 10px 20px !important; position: relative; top: 0; bottom: 0; right: 0; } /*Form Responsive CSS*/ @media only screen and (min-width: 1024px) and (max-width: 1366px){ .hs_email, .hs_contact_country { clear: both; } } @media only screen and (min-width: 992px) and (max-width: 1024px){ .hs_email, .hs_contact_country { clear: both; } } @media (min-width: 640px) and (max-width: 1120px) { .hbspt-form h1{font-size: 36px !important;} } @media screen and (min-width: 1120px) { .hbspt-form h1{font-size: 45px !important;margin-bottom: 17px !important;} .hbspt-form .gform_heading p{font-size: 17px !important;} } @media only screen and (min-width: 768px) and (max-width: 992px){ .hs_email, .hs_contact_country{ clear: both; } } @media (min-width: 767px) and (max-width: 1024px) { body .hubspot_signup_form .hs_email{width: 66% !important; float: left !important;} } @media screen and (max-width: 992px){ .assay-kit-hbspt-form .fl-col { width: 100%; } } @media screen and (max-width:812px){ .assay-kit-hbspt-form .fl-col { max-width: 100%; } .hbspt-form .hs-fieldtype-select .input:before{ right: 20px !important; } } @media screen and (max-width: 767px){ .hs_submit .actions{padding-top: 0px !important; margin-top: 0px !important;} body .hbspt-form fieldset.form-columns-2 .input {margin-right: 0px !important;} body fieldset.form-columns-3 .input{margin-right: 0px !important;} .hs-form-field .hs-input { width: 100% !important; } .hs-fieldtype-textarea .input{margin-right: 0px !important;} .hs_firstname, .hs_phone, .hs_development_area{ width: 100% !important; } } @media screen and (min-width: 640px){ .hbspt-form .hs_firstname, .kits_form_col .hs_email{ 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href="https://www.bioagilytix.com/speak-to-scientist/">Speak to a Scientist</a></div></div></div><div class="breadcrumbs"><span><span><a href="https://www.bioagilytix.com/"><i class="home-icon"></i><span class="screen-reader-text">Home</span></a></span> <span class="breadcrumb-separator"></span> <span><a href="https://www.bioagilytix.com/techniques/">Techniques & Platforms</a></span> <span class="breadcrumb-separator"></span> <span><a href="https://www.bioagilytix.com/techniques/instrumentation/">Instrumentation</a></span> <span class="breadcrumb-separator"></span> <span class="breadcrumb_last" aria-current="page">ELISpot</span></span></div></div></section><div class="wp-block-group alignwide is-layout-constrained wp-container-core-group-is-layout-1 wp-block-group-is-layout-constrained"><p class="has-text-align-center has-large-font-size">BioAgilytix offers Enzyme-Linked Immunosorbent Spot (ELISpot) assays at multiple global locations. With the ability to qualify and validate assays for nonregulated or regulated work, BioAgilytix’s ELISpot platform provides robust data for monitoring cell-mediated immune responses to biologics, cell therapies, and gene therapies during clinical and preclinical testing. <strong> </strong></p></div><div id="block_a0a2b4dd4b99a275814618265d281c8f" class="wp-block em-media-content em-mt--none em-mb--lg alignfull"><div class="em-media-content__inner em-media-content__inner--media-right has-gray-xxl-background-color em-media-content__inner--content-has-padding"><div class="em-media-content__media em-media-content__media--right"> <img fetchpriority="high" decoding="async" src="https://www.bioagilytix.com/wp-content/uploads/2023/08/t-cell-dark2-1380x776.jpg" alt="t cell" class="object-fit--cover" srcset="https://www.bioagilytix.com/wp-content/uploads/2023/08/t-cell-dark2-1380x776.jpg 1380w, https://www.bioagilytix.com/wp-content/uploads/2023/08/t-cell-dark2-1120x630.jpg 1120w, https://www.bioagilytix.com/wp-content/uploads/2023/08/t-cell-dark2-850x478.jpg 850w, https://www.bioagilytix.com/wp-content/uploads/2023/08/t-cell-dark2-640x360.jpg 640w" width="1380" height="776"></div><div class="em-media-content__content em-media-content__content--left em-media-content__content--add-padding"><h2 class="h2"><strong><strong>BioAgilytix’s ELISpot expertise</strong></strong></h2><p class="">The ELISpot platform provides a method for accurately detecting and enumerating antigen-specific immune cells with a high degree of sensitivity. BioAgilytix’s ELISpot assays can be qualified and validated to support regulated studies, and our team has a proven track record of leveraging this platform to deliver high-quality results. Speak with one of our ELISpot experts about your scientific requirements and learn how the features of this platform can be optimally leveraged for bioanalysis in support of your therapeutic program.  </p></div></div></div><div id="block_1e6774683cdc03116dca993ff8d4759e" class="wp-block em-media-content em-mt--md em-mb--md alignwide"><div class="em-media-content__inner em-media-content__inner--media-left has-none-background-color em-media-content__inner--content-has-padding"><div class="em-media-content__media em-media-content__media--left"> <noscript><img decoding="async" src="https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1092921812.jpg" alt="cancer cells 3d illustration" class="object-fit--cover" srcset="https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1092921812.jpg 1000w, https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1092921812-300x169.jpg 300w, https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1092921812-768x432.jpg 768w, https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1092921812-850x478.jpg 850w, https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1092921812-640x360.jpg 640w, https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1092921812-100x56.jpg 100w" width="1000" height="563"></noscript><img decoding="async" src='data:image/svg+xml,%3Csvg%20xmlns=%22http://www.w3.org/2000/svg%22%20viewBox=%220%200%201000%20563%22%3E%3C/svg%3E' data-src="https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1092921812.jpg" alt="cancer cells 3d illustration" class="lazyload object-fit--cover" data-srcset="https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1092921812.jpg 1000w, https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1092921812-300x169.jpg 300w, https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1092921812-768x432.jpg 768w, https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1092921812-850x478.jpg 850w, https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1092921812-640x360.jpg 640w, https://www.bioagilytix.com/wp-content/uploads/2023/08/shutterstock_1092921812-100x56.jpg 100w" width="1000" height="563"></div><div class="em-media-content__content em-media-content__content--right em-media-content__content--add-padding"><h2 class="h2"><strong><strong>ELISpot applications for determining cell-mediated immunity  </strong></strong></h2><p class="">The ELISpot platform has become a leading technology for monitoring cell-mediated immunity in the regulated bioanalytical space. ELISpot assays provide quantitative information regarding the number of cells secreting specific cytokines as part of a cellular immune response directed toward therapeutic proteins or peptides. </p><p class="">We use ELISpot to visualize individual cells that secrete cytokines after stimulation. By adding a known concentration of a patient’s immune cells, such as purified peripheral blood mononuclear cells (PBMCs), the frequency of antigen-specific cells can be determined after in vitro stimulation with the antigen of interest. For cellular immunogenicity, proteins or peptides derived from the therapeutic can be used for stimulation, which provides information on the drug-specific immune response. </p><p class="">At BioAgilytix, we most frequently utilize ELISpot in our work with cellular immune responses or T cell responses to cell and gene therapy products. However, we have also supported determination of antigenic epitopes, and antigen-specific B cell or plasma cell prevalence. For bioanalytical assessment of gene therapies, ELISpot can be used to assess cellular immune responses against the vector and transgene. With cell therapy products, the response against unique sequences in the CAR construct or other novel proteins expressed on the therapeutic can be evaluated.   </p></div></div></div><div class="wp-block-emagine-background alignfull has-quaternary-background-color has-background-dim em-pt--xl em-pb--xl em-mt--lg"><div class="wp-block-emagine-background__content"><div class="wp-block-emagine-background__content-inner"><div class="wp-block-emagine-grid alignwide wp-block-emagine-grid--left-heavy"><div class="wp-block-emagine-grid__inner em-grid-lgGutter-equalHeight-left-alternateRows"><div class="wp-block-emagine-column em-col-8_sm-12"><h6 class="has-primary-l-color has-text-color h6"><strong><strong><strong>Key ELISpot Benefits: </strong></strong></strong></h6><h2 class="has-em-white-color has-text-color h2 em-mt--xs"><strong><strong><strong>Exceptional sensitivity and single cell detection  </strong></strong></strong></h2><p class="">The ELISpot platform offers several distinct advantages over other methods. First, the cells used in the assay can be isolated and cryopreserved, negating the need for fresh samples at the point of analysis. Stimulation of the cells then takes place in the final assay plate, which streamlines the laboratory procedure.  </p><p class="">A cell that is activated by the proteins or peptides added to the wells during stimulation will produce cytokines, which are secreted into the surrounding matrix. The wells of the assay plate have a membrane coated with antibodies that captures the secreted cytokines. When the cells are removed from the plate at the end of the assay, each activated cell can then be visualized as spots of cytokines left behind on the membrane. By visualizing each individual activated cell, this methodology offers exceptional sensitivity and provides a direct, quantitative readout of antigen-specific cell frequency. </p><p class="">Selecting the specific cytokine — or cytokines for multiplexed assays — detected in this assay format can also provide an indication of the immune cell types that are activated in response to a stimulant. As such, ELISpot assays are able to provide informative data sets for evaluating and predicting the type of immune responses occurring in the patient. </p></div><div class="wp-block-emagine-column em-col-4_sm-12"><h5 class="has-em-white-color has-text-color h5"><strong>Benefits of ELISpot assays:</strong> </h5><ul class="wp-block-list"><li class="">High sensitivity </li><li class="">Single cell detection and enumeration </li><li class="">Scalability for higher throughput </li></ul></div></div></div></div></div></div><div id="block_aa23ec62ab06d42a1f0cc9f0b803a20d" class="wp-block em-media-content alignwide"><div class="em-media-content__inner em-media-content__inner--media-left has-none-background-color em-media-content__inner--content-has-padding"><div class="em-media-content__media em-media-content__media--left"> <noscript><img decoding="async" src="https://www.bioagilytix.com/wp-content/uploads/2023/08/immunospot.jpeg" alt="immunospot" class="object-fit--contain" srcset="https://www.bioagilytix.com/wp-content/uploads/2023/08/immunospot.jpeg 600w, https://www.bioagilytix.com/wp-content/uploads/2023/08/immunospot-300x300.jpeg 300w, https://www.bioagilytix.com/wp-content/uploads/2023/08/immunospot-150x150.jpeg 150w, https://www.bioagilytix.com/wp-content/uploads/2023/08/immunospot-100x100.jpeg 100w" width="600" height="600"></noscript><img decoding="async" src='data:image/svg+xml,%3Csvg%20xmlns=%22http://www.w3.org/2000/svg%22%20viewBox=%220%200%20600%20600%22%3E%3C/svg%3E' data-src="https://www.bioagilytix.com/wp-content/uploads/2023/08/immunospot.jpeg" alt="immunospot" class="lazyload object-fit--contain" data-srcset="https://www.bioagilytix.com/wp-content/uploads/2023/08/immunospot.jpeg 600w, https://www.bioagilytix.com/wp-content/uploads/2023/08/immunospot-300x300.jpeg 300w, https://www.bioagilytix.com/wp-content/uploads/2023/08/immunospot-150x150.jpeg 150w, https://www.bioagilytix.com/wp-content/uploads/2023/08/immunospot-100x100.jpeg 100w" width="600" height="600"></div><div class="em-media-content__content em-media-content__content--right em-media-content__content--add-padding"><h3 class="h3"><strong><strong><strong>How the ELISpot platform works </strong></strong></strong></h3><p class="">BioAgilytix utilizes ImmunoSpot<sup>®</sup> ELISpot plate readers equipped with top-end optics to enable high-resolution detection. These systems support traditional enzymatic assays as well as fluorescence and bright-field imaging. The ImmunoSpot software can accommodate single- or double-color enzymatic assays (blue spot/single analyte or blue and red spot/dual analyte) as well as FluoroSpot assays (single or multiplex up to four analytes). An automated plate loader also enables scaling up for higher throughput applications. The instruments and software at BioAgilytix have been installed and validated to be 21 CFR Part 11 compliant, enabling full use of the platform for regulated assay support. </p></div></div></div></article></main><footer class="site-footer site-footer--has-logo site-footer--align-brand-left"><div class="deep-footer clearfix"><div class="alignwide"><div class="em-grid"><div class="em-col-6_md-12"><div class="deep-footer__brand"><div class="deep-footer__logo" style="max-width: 300px"> <a href="https://www.bioagilytix.com"><div class="em-image"><div class="em-image__ratio" style="padding-top:21.420572708367%;"> <svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 187.53 40.17" width="187.53px" height="40.17px" class=""><g fill="none"><g fill="#fff"><path d="M12.04 18.13v-.14c2.53-1.16 3.8-2.91 3.8-5.23 0-.87-.16-1.61-.49-2.24s-.78-1.11-1.36-1.45c-.64-.38-1.4-.65-2.28-.8-.66-.11-1.51-.17-2.56-.17H0v1.02c.35.1.63.22.83.35s.35.29.45.5.16.47.18.8.04.73.04 1.22v13.3c0 .5-.01.9-.04 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