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mice</title> <meta name="description" content="Search results for: 5XFAD mice"> <meta name="keywords" content="5XFAD mice"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none navbar-toggler ml-auto" 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id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="5XFAD mice"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 525</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: 5XFAD mice</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">525</span> Maresin Like 1 Treatment: Curbing the Pathogenesis of Behavioral Dysfunction and Neurodegeneration in Alzheimer's Disease Mouse Model</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Yan%20Lu">Yan Lu</a>, <a href="https://publications.waset.org/abstracts/search?q=Song%20Hong"> Song Hong</a>, <a href="https://publications.waset.org/abstracts/search?q=Janakiraman%20Udaiyappan"> Janakiraman Udaiyappan</a>, <a href="https://publications.waset.org/abstracts/search?q=Aarti%20Nagayach"> Aarti Nagayach</a>, <a href="https://publications.waset.org/abstracts/search?q=Quoc-Viet%20A.%20Duong"> Quoc-Viet A. Duong</a>, <a href="https://publications.waset.org/abstracts/search?q=Masao%20Morita"> Masao Morita</a>, <a href="https://publications.waset.org/abstracts/search?q=Shun%20Saito"> Shun Saito</a>, <a href="https://publications.waset.org/abstracts/search?q=Yuichi%20Kobayashi"> Yuichi Kobayashi</a>, <a href="https://publications.waset.org/abstracts/search?q=Yuhai"> Yuhai</a>, <a href="https://publications.waset.org/abstracts/search?q=Zhao"> Zhao</a>, <a href="https://publications.waset.org/abstracts/search?q=Hongying%20Peng"> Hongying Peng</a>, <a href="https://publications.waset.org/abstracts/search?q=Nicholas%20B.%20Pham"> Nicholas B. Pham</a>, <a href="https://publications.waset.org/abstracts/search?q=Walter%20J%20Lukiw"> Walter J Lukiw</a>, <a href="https://publications.waset.org/abstracts/search?q=Christopher%20A.%20Vuong"> Christopher A. Vuong</a>, <a href="https://publications.waset.org/abstracts/search?q=Nicolas%20G.%20Bazan"> Nicolas G. Bazan</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Aims: Neurodegeneration and behavior dysfunction occurs in patients with Alzheimer's Disease (AD), and as the disease progresses many patients develop cognitive impairment. 5XFAD mouse model of AD is widely used to study AD pathogenesis and treatment. This study aimed to investigate the effect of maresin like 1 (MaR-L1) treatment in AD pathology using 5XFAD mice. Methods: We tested 12-month-old male 5XFAD mice and wild type control mice treated with MaR-L1 in a battery of behavioral tasks. We performed open field test, beam walking test, clasping test, inverted grid test, acetone test, marble burring test, elevated plus maze test, cross maze test and novel object recognition test. We also studied neuronal loss, amyloid β burden, and inflammation in the brains of 5XFAD mice using immunohistology and Western blotting. Results: MaR-L1 treatment to the 5XFAD mice showed improved cognitive function of 5XFAD mice. MaR-L1 showed decreased anxiety behavior in open field test and marble burring test, increased muscular strength in the beam walking test, clasping test and inverted grid test. Cognitive function was improved in MaR-L1 treated 5XFAD mice in the novel object recognition test. MaR-L1 prevented neuronal loss and aberrant inflammation. Conclusion: Our finding suggests that behavioral abnormalities were normalized by the administration of MaR-L1 and the neuroprotective role of MaR-L1 in the AD. It also indicates that MaR-L1 treatment is able to prevent and or ameliorate neuronal loss and aberrant inflammation. Further experiments to validate the results are warranted using other AD models in the future. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Alzheimer%27s%20disease" title="Alzheimer's disease">Alzheimer's disease</a>, <a href="https://publications.waset.org/abstracts/search?q=motor%20and%20cognitive%20behavior" title=" motor and cognitive behavior"> motor and cognitive behavior</a>, <a href="https://publications.waset.org/abstracts/search?q=5XFAD%20mice" title=" 5XFAD mice"> 5XFAD mice</a>, <a href="https://publications.waset.org/abstracts/search?q=Maresin%20Like%201" title=" Maresin Like 1"> Maresin Like 1</a>, <a href="https://publications.waset.org/abstracts/search?q=microglial%20cell" title=" microglial cell"> microglial cell</a>, <a href="https://publications.waset.org/abstracts/search?q=astrocyte" title=" astrocyte"> astrocyte</a>, <a href="https://publications.waset.org/abstracts/search?q=neurodegeneration" title=" neurodegeneration"> neurodegeneration</a>, <a href="https://publications.waset.org/abstracts/search?q=inflammation" title=" inflammation"> inflammation</a>, <a href="https://publications.waset.org/abstracts/search?q=resolution%20of%20inflammation" title=" resolution of inflammation"> resolution of inflammation</a> </p> <a href="https://publications.waset.org/abstracts/131955/maresin-like-1-treatment-curbing-the-pathogenesis-of-behavioral-dysfunction-and-neurodegeneration-in-alzheimers-disease-mouse-model" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/131955.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">178</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">524</span> Trigonella foenum-graecum Seeds Extract as Therapeutic Candidate for Treatment of Alzheimer's Disease</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mai%20M.%20Farid">Mai M. Farid</a>, <a href="https://publications.waset.org/abstracts/search?q=Ximeng%20Yang"> Ximeng Yang</a>, <a href="https://publications.waset.org/abstracts/search?q=Tomoharu%20Kuboyama"> Tomoharu Kuboyama</a>, <a href="https://publications.waset.org/abstracts/search?q=Yuna%20Inada"> Yuna Inada</a>, <a href="https://publications.waset.org/abstracts/search?q=Chihiro%20Tohda"> Chihiro Tohda</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Intro: Trigonella foenum-graecum (Fenugreek), from Fabaceae family is a well-known plant traditionally used as food and medicine. Many pharmacological effects of Trigonella foenum- graecum seeds extract (TF extract) were evaluated such as anti-diabetic, anti-tumor and anti-dementia effects using in vivo models. Regarding the anti-dementia effects of TF extract, diabetic rats, aluminum chloride-induced amnesia rats and scopolamine-injected mice were used previously for evaluation, which are not well established as Alzheimer’s disease models. In addition, those previous studies, active constituents in TF extract for memory function were not identified. Method: This study aimed to clarify the effect of TF extract on Alzheimer’s disease model, 5XFAD mouse that overexpresses mutated APP and PS1 genes and determine the major active constituent in the brain after oral intake of TF extract. Results: Trigonelline was detected in the cerebral cortex of 5XFAD mice after 24 hours of oral administration of TF extract by LC-MS/MS. Oral administration of TF extract for 17 days improved object location memory in 5XFAD mice. Conclusion: These results suggest that TF extract and its active constituents could be an expected therapeutic candidate for Alzheimer’s disease. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Alzheimer%27s%20disease" title="Alzheimer's disease">Alzheimer's disease</a>, <a href="https://publications.waset.org/abstracts/search?q=LC-MS%2FMS" title=" LC-MS/MS"> LC-MS/MS</a>, <a href="https://publications.waset.org/abstracts/search?q=memory%20recovery" title=" memory recovery"> memory recovery</a>, <a href="https://publications.waset.org/abstracts/search?q=Trigonella%20foenum-graecum%20Seeds" title=" Trigonella foenum-graecum Seeds"> Trigonella foenum-graecum Seeds</a>, <a href="https://publications.waset.org/abstracts/search?q=5XFAD%20mice" title=" 5XFAD mice"> 5XFAD mice</a> </p> <a href="https://publications.waset.org/abstracts/131399/trigonella-foenum-graecum-seeds-extract-as-therapeutic-candidate-for-treatment-of-alzheimers-disease" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/131399.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">147</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">523</span> Trigonelline: A Promising Compound for The Treatment of Alzheimer's Disease</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mai%20M.%20Farid">Mai M. Farid</a>, <a href="https://publications.waset.org/abstracts/search?q=Ximeng%20Yang"> Ximeng Yang</a>, <a href="https://publications.waset.org/abstracts/search?q=Tomoharu%20Kuboyama"> Tomoharu Kuboyama</a>, <a href="https://publications.waset.org/abstracts/search?q=Chihiro%20Tohda"> Chihiro Tohda</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Trigonelline is a major alkaloid component derived from Trigonella foenum-graecum L. (fenugreek) and has been reported before as a potential neuroprotective agent, especially in Alzheimer’s disease (AD). However, the previous data were unclear and used model mice were not well established. In the present study, the effect of trigonelline on memory function was investigated in Alzheimer’s disease transgenic model mouse, 5XFAD which overexpresses the mutated APP and PS1 genes. Oral administration of trigonelline for 14 days significantly enhanced object recognition and object location memories. Plasma and cerebral cortex were isolated at 30 min, 1h, 3h, and 6 h after oral administration of trigonelline. LC-MS/MS analysis indicated that trigonelline was detected in both plasma and cortex from 30 min after, suggesting good penetration of trigonelline into the brain. In addition, trigonelline significantly ameliorated axonal and dendrite atrophy in Amyloid β-treated cortical neurons. These results suggest that trigonelline could be a promising therapeutic candidate for AD. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=alzheimer%E2%80%99s%20disease" title="alzheimer’s disease">alzheimer’s disease</a>, <a href="https://publications.waset.org/abstracts/search?q=cortical%20neurons" title=" cortical neurons"> cortical neurons</a>, <a href="https://publications.waset.org/abstracts/search?q=LC-MS%2FMS%20analysis" title=" LC-MS/MS analysis"> LC-MS/MS analysis</a>, <a href="https://publications.waset.org/abstracts/search?q=trigonelline" title=" trigonelline"> trigonelline</a> </p> <a href="https://publications.waset.org/abstracts/116264/trigonelline-a-promising-compound-for-the-treatment-of-alzheimers-disease" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/116264.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">147</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">522</span> Protective Role of Peroxiredoxin V against Ischemia/Reperfusion-Induced Acute Kidney Injury in Mice</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Eun%20Gyeong%20Lee">Eun Gyeong Lee</a>, <a href="https://publications.waset.org/abstracts/search?q=Ji%20Young%20Park"> Ji Young Park</a>, <a href="https://publications.waset.org/abstracts/search?q=Hyun%20Ae%20Woo"> Hyun Ae Woo</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Reactive oxygen species (ROS) production is involved in ischemia/reperfusion (I/R) injury in kidney of mice. Oxidative stress develops from an imbalance between ROS production and reduced antioxidant defenses. Many enzymatic and nonenzymatic antioxidant systems including peroxiredoxins (Prxs) are present in kidney to maintain an appropriate level of ROS and prevent oxidative damage. Prxs are a family of peroxidases that reduce peroxides, with a conserved cysteine residue serving as the site of oxidation by peroxides. In this study, we examined the protective role of Prx V against I/R-induced acute kidney injury (AKI) using Prx V wild type (WT) and knockout (KO) mice. We compared the response of Prx V WT and KO mice in mice model of I/R injury. Renal structure, functions, oxidative stress markers, protein levels of oxidative damage marker were worse in Prx V KO mice. Ablation of Prx V enhanced susceptibility to I/R-induced oxidative stress. Prx V KO mice were seen to have more severe renal damage than Prx V WT mice in mice model of I/R injury. Our results demonstrate that Prx V is protective against I/R-induced AKI. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=peroxiredoxin" title="peroxiredoxin">peroxiredoxin</a>, <a href="https://publications.waset.org/abstracts/search?q=ischemia%2Freperfusion" title=" ischemia/reperfusion"> ischemia/reperfusion</a>, <a href="https://publications.waset.org/abstracts/search?q=kidney" title=" kidney"> kidney</a>, <a href="https://publications.waset.org/abstracts/search?q=oxidative%20stress" title=" oxidative stress"> oxidative stress</a> </p> <a href="https://publications.waset.org/abstracts/47859/protective-role-of-peroxiredoxin-v-against-ischemiareperfusion-induced-acute-kidney-injury-in-mice" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/47859.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">386</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">521</span> Effect of Experience on Evacuation of Mice in Emergency Conditions</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Teng%20Zhang">Teng Zhang</a>, <a href="https://publications.waset.org/abstracts/search?q=Shenshi%20Huang"> Shenshi Huang</a>, <a href="https://publications.waset.org/abstracts/search?q=Gang%20Xu"> Gang Xu</a>, <a href="https://publications.waset.org/abstracts/search?q=Xuelin%20Zhang"> Xuelin Zhang</a>, <a href="https://publications.waset.org/abstracts/search?q=Shouxiang%20Lu"> Shouxiang Lu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> With the acceleration of urbanization and the increasing of the population in the city, the evacuation of pedestrians suffering from disaster environments such as fire in a room or other limited space becomes a vital issue in modern society. Mice have been used in experimental crowd evacuation in recent years for its good similarities to human in physical structure and stress reaction. In this study, the effect of experience or memory on the collective behavior of mice was explored. To help mice familiarize themselves with the design of the space and the stimulus caused by smoke, we trained them repeatedly for 2 days so that they can escape from the emergency conditions as soon as possible. The escape pattern, trajectories, walking speed, turning angle and mean individual escape time of mice in each training trail were analyzed. We found that mice can build memory quickly after the first trial on the first day. On the second day, the evacuation of mice was maintained in a stable and efficient state. Meanwhile, the group with size of 30 (G30) had a shorter mean individual escape time compared with G12. Furthermore, we tested the experience of evacuation skill of mice after several days. The results showed that the mice can hold the experience or memory over 3 weeks. We proposed the importance of experience of evacuation skill and the research of training methods in experimental evacuation of mice. The results can deepen our understanding of collective behavior of mice and conduce to the establishment of animal models in the study of pedestrian crowd dynamics in emergency conditions. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=experience" title="experience">experience</a>, <a href="https://publications.waset.org/abstracts/search?q=evacuation" title=" evacuation"> evacuation</a>, <a href="https://publications.waset.org/abstracts/search?q=mice" title=" mice"> mice</a>, <a href="https://publications.waset.org/abstracts/search?q=group%20size" title=" group size"> group size</a>, <a href="https://publications.waset.org/abstracts/search?q=behavior" title=" behavior"> behavior</a> </p> <a href="https://publications.waset.org/abstracts/100161/effect-of-experience-on-evacuation-of-mice-in-emergency-conditions" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/100161.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">268</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">520</span> Evaluation of the Laser and Partial Vibration Stimulation on Osteoporosis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ji%20Hyung%20Park">Ji Hyung Park</a>, <a href="https://publications.waset.org/abstracts/search?q=Dong-Hyun%20Seo"> Dong-Hyun Seo</a>, <a href="https://publications.waset.org/abstracts/search?q=Young-Jin%20Jung"> Young-Jin Jung</a>, <a href="https://publications.waset.org/abstracts/search?q=Han%20Sung%20Kim"> Han Sung Kim</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The aim of this study is to evaluate the effects of the laser and partial vibration stimulation on the mice tibia with morphological characteristics. Twenty female C57BL/6 mice (12 weeks old) were used for the experiment. The study was carried out on four groups of animals each consisting of five mice. Four groups of mice were ovariectomized. Animals were scanned at 0 and 2 weeks after ovariectomy by using micro-computed tomography to estimate morphological characteristics of tibial trabecular bone. Morphological analysis showed that structural parameters of multi-stimuli group appear significantly better phase in BV/TV, BS/BV, Tb.Th, Tb.N, Tb.Sp, and Tb.pf than single stimulation groups. However, single stimulation groups didn’t show significant effect on tibia with Sham group. This study suggests that multi-stimuli may restrain the change as the degenerate phase on osteoporosis in the mice tibia. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=laser" title="laser">laser</a>, <a href="https://publications.waset.org/abstracts/search?q=partial%20vibration" title=" partial vibration"> partial vibration</a>, <a href="https://publications.waset.org/abstracts/search?q=osteoporosis" title=" osteoporosis"> osteoporosis</a>, <a href="https://publications.waset.org/abstracts/search?q=in-vivo%20micro-CT" title=" in-vivo micro-CT"> in-vivo micro-CT</a>, <a href="https://publications.waset.org/abstracts/search?q=mice" title=" mice "> mice </a> </p> <a href="https://publications.waset.org/abstracts/25012/evaluation-of-the-laser-and-partial-vibration-stimulation-on-osteoporosis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/25012.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">515</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">519</span> Cholesterol Modulating Properties of a Proprietary Extract from Phyllanthus spp on Hypercholesteraemic Mice Models</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Anne%20R.%20Fernandez">Anne R. Fernandez</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohammad%20Akmal%20Adnan"> Mohammad Akmal Adnan</a>, <a href="https://publications.waset.org/abstracts/search?q=Tanes%20Prasat"> Tanes Prasat</a>, <a href="https://publications.waset.org/abstracts/search?q=Indu%20Bala%20Jaganath"> Indu Bala Jaganath</a>, <a href="https://publications.waset.org/abstracts/search?q=Brian%20Kirby"> Brian Kirby</a>, <a href="https://publications.waset.org/abstracts/search?q=Kamalan%20Jeevaratnam"> Kamalan Jeevaratnam</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Introduction: Plants from the Phyllantus genus have been used indigenously for the treatment of a variety of ailments for generations. A cocktail of phytonutrients prepared from a plant of the genus Phyllanthus has demonstrated the potential to alleviate ailments which include cardiovascular disorders. In this study, we investigated the cholesterol modulating properties of a highly purified proprietary extract of a Phyllanthus species in hypercholesteraemic mice. Methods: Hypercholesteraemia was induced in ICR mice by ad-libitum feeding of high fat diet daily for six weeks. The mice were then divided into 3 groups and force fed with 10mg/kg of atorvastatin, 200mg/kg of the proprietary Phyllanthus extract and water respectively. Blood samples were taken at the end of fourth week of treatment by a tail prick. At the end of the eighth week of treatment, mice were sacrificed and serum levels of total cholesterol, high-density lipoprotein (HDL), low-density lipoprotein (LDL) and triglycerides were measured. Results: The mean cholesterol levels in the mice fed with high fat diet were 44% (p < 0.05) higher than the mice on normal diet thus validating the model developed. The plasma HDL was significantly elevated in mice treated with the formulation (p ˂ 0.05) in comparison to the statin-treated and control mice. The total cholesterol levels in the mice treated with the proprietary extract were reduced significantly (p < 0.05) at the end of 4 weeks of treatment in comparison to the mice treated with atorvastatin. By the end of 8 weeks of treatment, there was no significant difference in the cholesterol levels of the mice in all groups. Conclusion: These results demonstrate that this proprietary extract from Phyllanthus species has the beneficial effect of reducing total cholesterol level more rapidly than atorvastatin and increasing HDL levels. Since an increase in the HDL cholesterol can reduce the risk of heart disease, this proprietary extract is a useful and safe therapeutic option compared to atorvastatin. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=high-density%20lipoprotein" title="high-density lipoprotein">high-density lipoprotein</a>, <a href="https://publications.waset.org/abstracts/search?q=hypercholesteraemic%20mice%20model" title=" hypercholesteraemic mice model"> hypercholesteraemic mice model</a>, <a href="https://publications.waset.org/abstracts/search?q=ICR%20mice" title=" ICR mice"> ICR mice</a>, <a href="https://publications.waset.org/abstracts/search?q=Phyllanthus%20spp." title=" Phyllanthus spp. "> Phyllanthus spp. </a> </p> <a href="https://publications.waset.org/abstracts/35450/cholesterol-modulating-properties-of-a-proprietary-extract-from-phyllanthus-spp-on-hypercholesteraemic-mice-models" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/35450.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">444</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">518</span> Effects of Exercise on Klotho Expression and Klotho DNA Methylation in Obese Mice</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Yao%20Huang">Yao Huang</a>, <a href="https://publications.waset.org/abstracts/search?q=Hongjie%20Yu"> Hongjie Yu</a>, <a href="https://publications.waset.org/abstracts/search?q=Fangrong%20Xu"> Fangrong Xu</a>, <a href="https://publications.waset.org/abstracts/search?q=Longbiao%20Cai"> Longbiao Cai</a>, <a href="https://publications.waset.org/abstracts/search?q=Qiqiang%20He"> Qiqiang He</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The Klotho gene has been found to be involved in cardiovascular health, and epigenetic mechanism has risen as good candidates to understand the role of lifestyle factors in obesity. The aim of this study was to investigate the effect of exercise intervention on the expression and DNA methylation of Klotho gene in high-fat diet induced obese mice. C57BL/6 male mice were fed a normal diet (ND) or a high-fat diet (HFD) for 12 weeks. HFD induced obese mice were divided into secondary group (SED) and exercise group (EX) randomly. The treadmill exercise was performed in EX group for 8 weeks. The expression and DNA methylation of Klotho were evaluated by Western blot, RT-PCR, and Methylation-specific PCR. Results indicated that Klotho protein and mRNA expression were significantly lower in the SED group than those in the ND and EX groups (P<0.01), whereas no significant difference, was found between ND group and EX group (P>0.05). Furthermore, mice in the ND group and SED group showed significantly lower levels of completely methylated Klotho DNA in ND group (0%) and SED group (50%) compared with the EX group (90%), and unmethylated Klotho DNA level in ND group (80%) was significantly higher than those in the SED (0%) and EX (0%) groups. These results suggested that exercise leads to increased Klotho expression and reduced Klotho DNA methylation level in HFD induced obese mice. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=DNA%20methylation" title="DNA methylation">DNA methylation</a>, <a href="https://publications.waset.org/abstracts/search?q=exercise%20intervention" title=" exercise intervention"> exercise intervention</a>, <a href="https://publications.waset.org/abstracts/search?q=klotho" title=" klotho"> klotho</a>, <a href="https://publications.waset.org/abstracts/search?q=obese%20mice" title=" obese mice "> obese mice </a> </p> <a href="https://publications.waset.org/abstracts/56824/effects-of-exercise-on-klotho-expression-and-klotho-dna-methylation-in-obese-mice" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/56824.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">353</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">517</span> In vivo Anticandida Activity of Three Traditionally Used Medicinal Plants in East Africa</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Daniel%20P.%20Kisangau">Daniel P. Kisangau</a>, <a href="https://publications.waset.org/abstracts/search?q=Ken%20M.%20Hosea"> Ken M. Hosea</a>, <a href="https://publications.waset.org/abstracts/search?q=Herbert%20V.%20M.%20Lyaruu"> Herbert V. M. Lyaruu</a>, <a href="https://publications.waset.org/abstracts/search?q=Cosam%20C.%20Josep"> Cosam C. Josep</a>, <a href="https://publications.waset.org/abstracts/search?q=Zakaria%20H.%20Mbwambo"> Zakaria H. Mbwambo</a>, <a href="https://publications.waset.org/abstracts/search?q=Pax%20J.%20Masimba"> Pax J. Masimba </a> </p> <p class="card-text"><strong>Abstract:</strong></p> Crude extracts of Dracaena steudneri bark (DSB), Sapium ellipticum bark (SEB) and Capparis erythrocarpos root (CER) were investigated for their antifungal activity in immunocompromised mice infected with Candida albicans in an in vivo mice infection model. The results revealed a substantial dose dependency in all treatments given, with mice survival to the end of the experiment correlating well to the dose levels. At a dose of 400 mg/kg, C. erythrocarpos was the most effective with mice survival of 60% and organ burden clearance ranging from 64.0%-99.9% (P<0.0001) in all treatments. At the same dose, the least effective plant was S. ellipticum which had a mice survival of 20% and organ burden clearance ranging from 78.0%-96.6 (P>0.05). Mice survival for D. steudneri was 30% with organ burden clearance ranging from 89.0%-99.9% (P<0.05). All mice receiving no active treatment died before ten days post infection. In all treatment groups, there was a steady decline in mean weights of mice immediately after immunosuppression followed by gradual recovery in some cases which appeared to be dose dependent a few days post infection. Thus, extracts of D. steudneri and C. erythrocarpos portrayed the most significant potential as sources of antifungal drugs. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=antifungal%20activity" title="antifungal activity">antifungal activity</a>, <a href="https://publications.waset.org/abstracts/search?q=medicinal%20plants" title=" medicinal plants"> medicinal plants</a>, <a href="https://publications.waset.org/abstracts/search?q=candida%20albicans" title=" candida albicans"> candida albicans</a>, <a href="https://publications.waset.org/abstracts/search?q=East%20Africa" title=" East Africa"> East Africa</a> </p> <a href="https://publications.waset.org/abstracts/14067/in-vivo-anticandida-activity-of-three-traditionally-used-medicinal-plants-in-east-africa" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/14067.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">505</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">516</span> A Recombinant Group a Streptococcus (GAS-2W) Strain Elicits Protective Immunity in Mice through Induction of an IFN-γ Dependent Humoral Response</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Shiva%20Emami">Shiva Emami</a>, <a href="https://publications.waset.org/abstracts/search?q=Jenny%20Persson"> Jenny Persson</a>, <a href="https://publications.waset.org/abstracts/search?q=Bengt%20Johansson%20Lindbom"> Bengt Johansson Lindbom</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Group A streptococcus (GAS) is a prevalent human pathogen, causing a wide range of infections and diseases. One of the most well-known virulence factors in GAS is M protein, a surface protein that facilitates bacterial invasion. In this study, we used a recombinant GAS strain (GAS-2W) expressing M protein containing a hyper immunogenic peptide (2W). Mice were immunized three times with heat-killed-GAS subcutaneously at three weeks intervals. Three weeks post last immunization, mice were challenged intraperitoneally with a lethal dose of live GAS. In order to investigate the impact of IFN-ƴ and antibodies in protection against GAS infection, we used a mouse model knock-out for IFN-ƴ (IFN-ƴ KO). We observed immunization with GAS-2W strain can increase protection against GAS infection in mice compared with the original GAS strain. Higher levels of antibodies against M1 protein were measured in GAS-2W-immunized mice. There was also a significant increase in IgG2c response in mice immunized with GAS2W. By using IFN-ƴ KO mice, we showed that not a high level of total IgG, but IgG2c was correlated with protection through the i.p challenge. It also emphasizes the importance of IFN-ƴ cytokine to combat GAS by isotype switching to IgG2c (which is opsonic for phagocytosis). Our data indicate the crucial role of IFN-ƴ in the protective immune response that, together with IgG2c, can induce protection against GAS. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Group%20A%20streptococcus" title="Group A streptococcus">Group A streptococcus</a>, <a href="https://publications.waset.org/abstracts/search?q=IgG2c" title=" IgG2c"> IgG2c</a>, <a href="https://publications.waset.org/abstracts/search?q=IFN-%CE%B3" title=" IFN-γ"> IFN-γ</a>, <a href="https://publications.waset.org/abstracts/search?q=protection" title=" protection"> protection</a> </p> <a href="https://publications.waset.org/abstracts/141555/a-recombinant-group-a-streptococcus-gas-2w-strain-elicits-protective-immunity-in-mice-through-induction-of-an-ifn-gh-dependent-humoral-response" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/141555.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">90</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">515</span> Methylprednisolone Injection Did Not Inhibit Anti-Hbs Response Following Hepatitis B Vaccination in Mice</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=P.%20O.%20Ughachukwu">P. O. Ughachukwu</a>, <a href="https://publications.waset.org/abstracts/search?q=P.%20O.%20Okonkwo"> P. O. Okonkwo</a>, <a href="https://publications.waset.org/abstracts/search?q=P.%20C.%20Unekwe"> P. C. Unekwe</a>, <a href="https://publications.waset.org/abstracts/search?q=J.%20O.%20Ogamba"> J. O. Ogamba</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: The prevalence of hepatitis B viral infection is high worldwide with liver cirrhosis and hepatocellular carcinoma as important complications. Cases of poor antibody response to hepatitis B vaccination abound. Immunosuppression, especially from glucocorticoids, is often cited as a cause of poor antibody response and there are documented evidences of irrational administration of glucocorticoids to children and adults. The study was, therefore, designed to find out if administration of glucocorticoids affects immune response to vaccination against hepatitis B in mice. Methods: Mice of both sexes were randomly divided into 2 groups. Daily intramuscular methylprednisolone injections, (15 mg kg-1), were given to the test group while sterile deionized water (0.1ml) was given to control mice for 30 days. On day 6 all mice were given 2 μg (0.1ml) hepatitis B vaccine and a booster dose on day 27. On day 34, blood samples were collected and analyzed for anti-HBs titres using enzyme-linked immunosorbent assay (ELISA). Statistical analysis was done using Graph Pad Prism 5.0 and the results taken as statistically significant at p value < 0.05. Results: There were positive serum anti-HBs responses in all mice groups but the differences in titres were not statistically significant. Conclusions: At the dosages and length of exposure used in this study, methylprednisolone injection did not significantly inhibit anti-HBs response in mice following immunization against hepatitis B virus. By extrapolation, methylprednisolone, when used in the usual clinical doses and duration of therapy, is not likely to inhibit immune response to hepatitis B vaccinations in man. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=anti-HBs" title="anti-HBs">anti-HBs</a>, <a href="https://publications.waset.org/abstracts/search?q=hepatitis%20B%20vaccine" title=" hepatitis B vaccine"> hepatitis B vaccine</a>, <a href="https://publications.waset.org/abstracts/search?q=immune%20response" title=" immune response"> immune response</a>, <a href="https://publications.waset.org/abstracts/search?q=methylprednisolone" title=" methylprednisolone"> methylprednisolone</a>, <a href="https://publications.waset.org/abstracts/search?q=mice" title=" mice"> mice</a> </p> <a href="https://publications.waset.org/abstracts/28711/methylprednisolone-injection-did-not-inhibit-anti-hbs-response-following-hepatitis-b-vaccination-in-mice" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/28711.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">323</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">514</span> Tactile Cues and Spatial Navigation in Mice</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Rubaiyea%20Uddin">Rubaiyea Uddin</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The hippocampus, located in the limbic system, is most commonly known for its role in memory and spatial navigation (as cited in Brain Reward and Pathways). It maintains an especially important role in specifically episodic and declarative memory. The hippocampus has also recently been linked to dopamine, the reward pathway’s primary neurotransmitter. Since research has found that dopamine also contributes to memory consolidation and hippocampal plasticity, this neurotransmitter is potentially responsible for contributing to the hippocampus’s role in memory formation. In this experiment we tested to see the effect of tactile cues on spatial navigation for eight different mice. We used a radial arm that had one designated 'reward' arm containing sucrose. The presence or absence of bedding was our tactile cue. We attempted to see if the memory of that cue would enhance the mice’s memory of having received the reward in that arm. The results from our study showed there was no significant response from the use of tactile cues on spatial navigation on our 129 mice. Tactile cues therefore do not influence spatial navigation. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=mice" title="mice">mice</a>, <a href="https://publications.waset.org/abstracts/search?q=radial%20arm%20maze" title=" radial arm maze"> radial arm maze</a>, <a href="https://publications.waset.org/abstracts/search?q=memory" title=" memory"> memory</a>, <a href="https://publications.waset.org/abstracts/search?q=spatial%20navigation" title=" spatial navigation"> spatial navigation</a>, <a href="https://publications.waset.org/abstracts/search?q=tactile%20cues" title=" tactile cues"> tactile cues</a>, <a href="https://publications.waset.org/abstracts/search?q=hippocampus" title=" hippocampus"> hippocampus</a>, <a href="https://publications.waset.org/abstracts/search?q=reward" title=" reward"> reward</a>, <a href="https://publications.waset.org/abstracts/search?q=sensory%20skills" title=" sensory skills"> sensory skills</a>, <a href="https://publications.waset.org/abstracts/search?q=Alzheimer%E2%80%99s" title=" Alzheimer’s"> Alzheimer’s</a>, <a href="https://publications.waset.org/abstracts/search?q=neurodegnerative%20disease" title=" neurodegnerative disease"> neurodegnerative disease</a> </p> <a href="https://publications.waset.org/abstracts/21710/tactile-cues-and-spatial-navigation-in-mice" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/21710.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">649</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">513</span> Dual-functional Peptide With Defective Interfering Genes Protecting Mice From Avian and Seasonal Influenza Virus Infection</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Hanjun%20Zhao">Hanjun Zhao</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Limited efficacy of current antivirals and antiviral-resistant mutations impair anti-influenza treatment. Here, we evaluated the in vitro and in vivo antiviral effect of three defective interfering genes (DIG-3) of influenza virus. Virus replication was significantly reduced in 293T and A549 cells transfected with DIG-3. Mice transfected with DIG-3 encoded by jetPEI-vector, as prophylaxis and therapeutics against A(H7N7) virus respectively, had significantly better survivals (80% and 50%) than control mice (0%). We further developed a dual-functional peptide TAT-P1, which delivers DIG-3 with high transfection efficiency and concomitantly exerts antiviral activity by preventing endosomal acidification. TAT-P1/DIG-3 was more effective than jetPEI/DIG-3 in treating A(H7N7) or A(H1N1)pdm09-infected mice and showed potent prophylactic protection on A(H7N7) or A(H1N1)pdm09-infected mice. The addition of P1 peptide, preventing endosomal acidification, could enhance the protection of TAT-P1/DIG-3 on A(H1N1)pdm09-infected mice. Dual-functional TAT-P1 with DIG-3 can effectively protect or treat mice infected by avian and seasonal influenza virus infection. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=antiviral%20peptide" title="antiviral peptide">antiviral peptide</a>, <a href="https://publications.waset.org/abstracts/search?q=dual-functional%20peptide" title=" dual-functional peptide"> dual-functional peptide</a>, <a href="https://publications.waset.org/abstracts/search?q=defective%20interfering%20genes" title=" defective interfering genes"> defective interfering genes</a>, <a href="https://publications.waset.org/abstracts/search?q=influenza%20virus" title=" influenza virus"> influenza virus</a> </p> <a href="https://publications.waset.org/abstracts/98170/dual-functional-peptide-with-defective-interfering-genes-protecting-mice-from-avian-and-seasonal-influenza-virus-infection" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/98170.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">122</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">512</span> Neutralizing Antibody Response against Inactivated FMDV Type O/IRN/2010 Vaccine by Electron Beam in BALB/C Mice</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=F.%20Motamedi%20Sedeh">F. Motamedi Sedeh</a>, <a href="https://publications.waset.org/abstracts/search?q=Sh.%20Chahardoli"> Sh. Chahardoli</a>, <a href="https://publications.waset.org/abstracts/search?q=H.%20Mahravani"> H. Mahravani</a>, <a href="https://publications.waset.org/abstracts/search?q=N.%20Harzandi"> N. Harzandi</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Sotoodeh"> M. Sotoodeh</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20K.%20Shafaei"> S. K. Shafaei </a> </p> <p class="card-text"><strong>Abstract:</strong></p> Foot-and-mouth disease virus (FMDV) is the most economically important disease of livestock. The aim of the study is inactivation of FMD virus type O/IRN/2010 by electron beam without antigenic changes as electron radio vaccine. The BALB/C mice were divided into three groups, each group containing five mice. Three groups of mice were inoculated with conventional vaccine and electron beam irradiated vaccine FMDV type O/IRN/2010 subcutaneously three weeks interval, the final group as negative control. The sera were separated from the blood samples of mice 14 days after last vaccination and tested for the presence of antibodies against FMDV type O/IRN/2010 by serum neutralization test. The Serum Neutralization Test (SNT) was carried out and antibody titration was calculated according to the Kraber protocol. The results of the SNT in three groups of mice showed the titration of neutralizing antibody in the vaccinated mice groups; electron radio vaccine and conventional vaccine were significantly higher than negative control group (P<0.05). Therefore, the radio vaccine is a good candidate to immunize animals against FMDV type O/IRN/2010. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=FMDV%20type%20O%2FIRN%2F2010" title="FMDV type O/IRN/2010">FMDV type O/IRN/2010</a>, <a href="https://publications.waset.org/abstracts/search?q=neutralizing%20antibody%20response" title=" neutralizing antibody response"> neutralizing antibody response</a>, <a href="https://publications.waset.org/abstracts/search?q=electron%20beam" title=" electron beam"> electron beam</a>, <a href="https://publications.waset.org/abstracts/search?q=radio%20vaccine" title=" radio vaccine"> radio vaccine</a> </p> <a href="https://publications.waset.org/abstracts/11949/neutralizing-antibody-response-against-inactivated-fmdv-type-oirn2010-vaccine-by-electron-beam-in-balbc-mice" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/11949.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">317</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">511</span> Molecular Mechanisms of Lipid Metabolism and Obesity Modulation by Caspase-1/11 and nlrp3 Inflammasome in Mice</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=L%C3%ADvia%20Pimentel%20Sant%27ana%20Dourado">Lívia Pimentel Sant'ana Dourado</a>, <a href="https://publications.waset.org/abstracts/search?q=Raquel%20Das%20Neves%20Almeida"> Raquel Das Neves Almeida</a>, <a href="https://publications.waset.org/abstracts/search?q=Lu%C3%ADs%20Henrique%20Costa%20Corr%C3%AAa%20Neto"> Luís Henrique Costa Corrêa Neto</a>, <a href="https://publications.waset.org/abstracts/search?q=Nayara%20Soares"> Nayara Soares</a>, <a href="https://publications.waset.org/abstracts/search?q=Kelly%20Grace%20Magalh%C3%A3es"> Kelly Grace Magalhães</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Introduction: Obesity and high-fat diet intake have a crucial impact on immune cells and inflammatory profile, highlighting an emerging realization that obesity is an inflammatory disease. In the present work, we aimed to characterize the role of caspase-1/11 and NLRP3 inflammasome in the establishment of mice obesity and modulation of inflammatory lipid metabolism induced by high fat diet intake. Methods and results: Wild type, caspase-1/11 and NLRP3 knockout mice were fed with standard fat diet (SFD) or high fat diet (HFD) for 90 days. The weight of animals was measured weekly to monitor the weight gain. After 90 days, the blood, peritoneal lavage cells, heart and liver were collected from mice studied here. Cytokines were measured in serum by ELISA and analyzed in spectrophotometry. Lipid antigen presentation molecule CD1d expression, reactive oxygen species (ROS) generation and lipid droplets biogenesis were analyzed in cells from mice peritoneal cavity by flow cytometry. Liver histopathology was performed for morphological evaluation of the organ. The absence of caspase-1/11, but not NLRP3, in mice fed with HFD favored the mice weight gain, increased liver size, induced development of hepatic steatosis and IL-12 secretion in mice compared to mice fed with SFD. In addition, caspase-1/11 knockout mice fed with HFD presented an increased CD1d molecule expression, as well as higher levels of lipid droplets biogenesis and ROS generation compared to wild type mice also fed with HFD. Conclusion: Our data suggest that caspase-1/11 knockout mice have greater susceptibility to obesity as well as increased activation of lipid metabolism and inflammatory markers. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=caspase%201" title="caspase 1">caspase 1</a>, <a href="https://publications.waset.org/abstracts/search?q=caspase%2011" title=" caspase 11"> caspase 11</a>, <a href="https://publications.waset.org/abstracts/search?q=inflamassome" title=" inflamassome"> inflamassome</a>, <a href="https://publications.waset.org/abstracts/search?q=obesity" title=" obesity"> obesity</a>, <a href="https://publications.waset.org/abstracts/search?q=lipids" title=" lipids"> lipids</a> </p> <a href="https://publications.waset.org/abstracts/58314/molecular-mechanisms-of-lipid-metabolism-and-obesity-modulation-by-caspase-111-and-nlrp3-inflammasome-in-mice" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/58314.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">319</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">510</span> Acute Oral Toxicity Study of Mystroxylon aethiopicum Root Bark Aqueous Extract in Albino Mice</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mhuji%20Kilonzo">Mhuji Kilonzo</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Acute oral toxicity of Mystroxylon aethiopicum root bark aqueous was evaluated in albino mice of either sex. In this study, five groups of mice were orally treated with doses of 1000, 2000, 3000, 4000 and 5000 mg/kg body weight of the crude extract. The mortality, signs of toxicity and body weights were observed individually for two weeks. At the end of the two weeks study, all animals were sacrificed, and the hematological and biochemical parameters, as well as organ weights relative to body weight of each animal, were determined. No mortality, signs of toxicity and abnormalities in vital organs were observed in the entire period of study for both treated and control groups of mice. Additionally, there were no significant changes (p > 0.05) in the blood hematology and biochemical analysis. However, the body weights of all mice increased significantly. The Mystroxylon aethiopicum root bark aqueous extract were found to have a high safe margin when administered orally. Hence, the extract can be utilized for pharmaceutical formulations. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=acute%20oral%20toxicity" title="acute oral toxicity">acute oral toxicity</a>, <a href="https://publications.waset.org/abstracts/search?q=albino%20mice" title=" albino mice"> albino mice</a>, <a href="https://publications.waset.org/abstracts/search?q=Mystroxylon%20aethiopicum" title=" Mystroxylon aethiopicum"> Mystroxylon aethiopicum</a>, <a href="https://publications.waset.org/abstracts/search?q=safety" title=" safety"> safety</a> </p> <a href="https://publications.waset.org/abstracts/63956/acute-oral-toxicity-study-of-mystroxylon-aethiopicum-root-bark-aqueous-extract-in-albino-mice" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/63956.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">289</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">509</span> Exposure to Tactile Cues Does Not Influence Spatial Navigation in 129 S1/SvLm Mice</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Rubaiyea%20Uddin">Rubaiyea Uddin</a>, <a href="https://publications.waset.org/abstracts/search?q=Rebecca%20Taylor"> Rebecca Taylor</a>, <a href="https://publications.waset.org/abstracts/search?q=Emily%20Levesque"> Emily Levesque</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The hippocampus, located in the limbic system, is most commonly known for its role in memory and spatial navigation (as cited in Brain Reward and Pathways). It maintains an especially important role in specifically episodic and declarative memory. The hippocampus has also recently been linked to dopamine, the reward pathway’s primary neurotransmitter. Since research has found that dopamine also contributes to memory consolidation and hippocampal plasticity, this neurotransmitter is potentially responsible for contributing to the hippocampus’s role in memory formation. In this experiment we tested to see the effect of tactile cues on spatial navigation for eight different mice. We used a radial arm that had one designated “reward” arm containing sucrose. The presence or absence of bedding was our tactile cue. We attempted to see if the memory of that cue would enhance the mice’s memory of having received the reward in that arm. The results from our study showed there was no significant response from the use of tactile cues on spatial navigation on our 129 mice. Tactile cues therefore do not influence spatial navigation. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=mice" title="mice">mice</a>, <a href="https://publications.waset.org/abstracts/search?q=radial%20arm%20maze" title=" radial arm maze"> radial arm maze</a>, <a href="https://publications.waset.org/abstracts/search?q=memory" title=" memory"> memory</a>, <a href="https://publications.waset.org/abstracts/search?q=spatial%20navigation" title=" spatial navigation"> spatial navigation</a>, <a href="https://publications.waset.org/abstracts/search?q=tactile%20cues" title=" tactile cues"> tactile cues</a>, <a href="https://publications.waset.org/abstracts/search?q=hippocampus" title=" hippocampus"> hippocampus</a>, <a href="https://publications.waset.org/abstracts/search?q=reward" title=" reward"> reward</a>, <a href="https://publications.waset.org/abstracts/search?q=sensory%20skills" title=" sensory skills"> sensory skills</a>, <a href="https://publications.waset.org/abstracts/search?q=Alzheimer%27s" title=" Alzheimer's"> Alzheimer's</a>, <a href="https://publications.waset.org/abstracts/search?q=neuro-degenerative%20diseases" title=" neuro-degenerative diseases"> neuro-degenerative diseases</a> </p> <a href="https://publications.waset.org/abstracts/17816/exposure-to-tactile-cues-does-not-influence-spatial-navigation-in-129-s1svlm-mice" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/17816.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">688</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">508</span> Hepatotoxicity Induced by Arsenic Trioxide in Adult Mice and Their Progeny</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Bouaziz%20H.">Bouaziz H.</a>, <a href="https://publications.waset.org/abstracts/search?q=Soudania%20N."> Soudania N.</a>, <a href="https://publications.waset.org/abstracts/search?q=Essafia%20M."> Essafia M.</a>, <a href="https://publications.waset.org/abstracts/search?q=Ben%20Amara%20I."> Ben Amara I.</a>, <a href="https://publications.waset.org/abstracts/search?q=Hakim%20A."> Hakim A.</a>, <a href="https://publications.waset.org/abstracts/search?q=Jamoussi%20K."> Jamoussi K.</a>, <a href="https://publications.waset.org/abstracts/search?q=Zeghal%20Km"> Zeghal Km</a>, <a href="https://publications.waset.org/abstracts/search?q=Zeghal%20N."> Zeghal N.</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In this investigation, we have evaluated the effects of arsenic trioxide on hepatic function in pregnant and lactating Swiss albino mice and their suckling pups. Experiments were carried out on female mice given 175 ppm As2O3 in their drinking water from the 14th day of pregnancy until day 14 after delivery. Our results showed a significant decrease in plasma levels of total protein and albumin, cholesterol and triglyceride in As2O3 treated mice and their pups. The hyperbilirubinemia and the increased plasma total alkaline phosphatase activity suggested the presence of cholestasis. Transaminase activities as well as lactate deshydrogenase activity in plasma, known as biomarkers of hepatocellular injury, were elevated indicating hepatic cells’damage after treatment with As2O3. Exposure to arsenic led to an increase of liver thiobarbituric acid reactive substances level along with a concomitant decrease in the activities of superoxide dismutase, catalase and glutathione peroxidase and in glutathione. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=antioxidant%20status" title="antioxidant status">antioxidant status</a>, <a href="https://publications.waset.org/abstracts/search?q=arsenic%20trioxide" title=" arsenic trioxide"> arsenic trioxide</a>, <a href="https://publications.waset.org/abstracts/search?q=hepatotoxicity" title=" hepatotoxicity"> hepatotoxicity</a>, <a href="https://publications.waset.org/abstracts/search?q=mice" title=" mice"> mice</a>, <a href="https://publications.waset.org/abstracts/search?q=oxidative%20stress" title=" oxidative stress"> oxidative stress</a> </p> <a href="https://publications.waset.org/abstracts/22776/hepatotoxicity-induced-by-arsenic-trioxide-in-adult-mice-and-their-progeny" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/22776.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">255</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">507</span> Concomitant Exposure of Bacoside A and Bromelain Relieves Dichlorvos Toxicity in Mice Serum </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sonam%20Agarwal">Sonam Agarwal</a>, <a href="https://publications.waset.org/abstracts/search?q=Renu%20Bist"> Renu Bist</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Current study emphasizes the toxic effects of dichlorvos on serum in terms of oxidative stress. Meanwhile, a protective action of bacoside A and bromelain was investigated against the biochemical alterations in serum. The experimental design included six groups of mice: saline was given as a vehicle to the control mice (group I). Mice belonging to groups II, III and IV, were administered with dichlorvos (40 mg/kg b.w.), bromelain and bacoside A, respectively. The fifth group received a combination of bromelain and bacoside A. In group VI, Bacoside A, and bromelain were administered 20 minutes prior to exposure of dichlorvos. Thiobarbituric acid reactive substances (TBARS), protein carbonyl content (PCC), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) and reduced glutathione (GSH) level were used as biochemical test of toxic action for dichlorvos intoxication. Significantly increased TBARS and PCC level in second group suggests that dichlorvos enhances the production of free radicals in serum of mice (p< 0.05). Antioxidants treatment significantly decreased the levels of TBARS and PCC (p< 0.05). Dichlorvos administration causes a significant reduction in the level of CAT, SOD, GPx and GSH (p< 0.05) which was restored significantly by co-administration of bromelain and Bacoside A in dichlorvos exposed mice (p< 0.05). Treatment of bromelain and Bacoside A in combination served as better scavengers of toxicity induced by dichlorvos. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bacoside%20A" title="bacoside A">bacoside A</a>, <a href="https://publications.waset.org/abstracts/search?q=bromelain" title=" bromelain"> bromelain</a>, <a href="https://publications.waset.org/abstracts/search?q=dichlorvos" title=" dichlorvos"> dichlorvos</a>, <a href="https://publications.waset.org/abstracts/search?q=serum" title=" serum"> serum</a> </p> <a href="https://publications.waset.org/abstracts/59409/concomitant-exposure-of-bacoside-a-and-bromelain-relieves-dichlorvos-toxicity-in-mice-serum" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/59409.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">349</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">506</span> Black-Legged Tick (Ixodes Scapularis) Impacts on Hematology and Ectoparasite Communities of Peromyscus Mice</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Erica%20Fellin">Erica Fellin</a>, <a href="https://publications.waset.org/abstracts/search?q=Albrecht%20Schulte-Hostedde"> Albrecht Schulte-Hostedde</a> </p> <p class="card-text"><strong>Abstract:</strong></p> As the climate warms, the black-legged tick’s (Ixodes scapularis) range expands further north in Ontario, Canada, reaching new host populations that have not previously interacted with this blood-feeding parasite. Peromyscus mice in these northern areas are unfamiliar and inexperienced to the effects of these ticks compared to their southern counterparts that have adapted to living with these organisms. The purpose of this study was to see if there is a difference in physiology between these two groups – deer mice living in areas where tick populations have established and deer mice living in black-legged tick-free environments – looking specifically to see if there is significant variation in hemoglobin levels, which can negatively impact how these mice function in their environment. Along with this, a comparison of the parasite community structure on these mice hosts was analyzed to see if ticks change the composition of these micro-environments. Blood samples were collected from individual mice from populations where black-legged ticks were either present or absent to assess haemoglobin levels. At the same time, ectoparasites were collected from these same mice to determine parasite loads and species diversity. Haemoglobin levels were found to be lower when tick loads were high, and parasite diversity appeared to be higher when ticks were absent. Since black-legged ticks are carriers of many pathogens that can be passed on to humans, including Lyme’s disease, it is important to understand their movement and distribution across Ontario as well as their interactions with their hosts (and co-occurring parasites) in their environments. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=community%20ecology" title="community ecology">community ecology</a>, <a href="https://publications.waset.org/abstracts/search?q=hematology" title=" hematology"> hematology</a>, <a href="https://publications.waset.org/abstracts/search?q=hosts" title=" hosts"> hosts</a>, <a href="https://publications.waset.org/abstracts/search?q=parasites" title=" parasites"> parasites</a> </p> <a href="https://publications.waset.org/abstracts/114218/black-legged-tick-ixodes-scapularis-impacts-on-hematology-and-ectoparasite-communities-of-peromyscus-mice" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/114218.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">140</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">505</span> Effect of Goat Milk Kefir and Soy Milk Kefir on IL-6 in Diabetes Mellitus Wistar Mice Models Induced by Streptozotocin and Nicotinamide</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Agatha%20Swasti%20Ayuning%20Tyas">Agatha Swasti Ayuning Tyas</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Hyperglycemia in Diabetes Mellitus (DM) is an important factor in cellular and vascular damage, which is caused by activation of C Protein Kinase, polyol and hexosamine track, and production of Advanced Glycation End-Products (AGE). Those mentioned before causes the accumulation of Reactive Oxygen Species (ROS). Oxidative stress increases the expression of proinflammatory factors IL-6 as one of many signs of endothelial disfunction. Genistein in soy milk has a high immunomodulator potential. Goat milk contains amino acids which have antioxidative potential. Fermented kefir has an anti-inflammatory activity which believed will also contribute in potentiating goat milk and soy milk. This study is a quasi-experimental posttest-only research to 30 Wistar mice. This study compared the levels of IL-6 between healthy Wistar mice group (G1) and 4 DM Wistar mice with intervention and grouped as follows: mice without treatment (G2), mice treated with 100% goat milk kefir (G3), mice treated with combination of 50% goat milk kefir and 50% soy milk kefir (G4), and mice treated with 100% soy milk kefir (G5). DM animal models were induced with Streptozotocin & Nicotinamide to achieve hyperglycemic condition. Goat milk kefir and soy milk kefir are given at a dose of 2 mL/kg body weight/day for four weeks to intervention groups. Blood glucose was analyzed by the GOD-POD principle. IL-6 was analyzed by enzyme-linked sandwich ELISA. The level of IL-6 in DM untreated control group (G2) showed a significant difference from the group treated with the combination of 50% goat milk kefir and 50% soy milk kefir (G3) (p=0,006) and the group treated with 100% soy milk kefir (G5) (p=0,009). Whereas the difference of IL-6 in group treated with 100% goat milk kefir (G3) was not significant (p=0,131). There is also synergism between glucose level and IL-6 in intervention groups treated with combination of 50% goat milk kefir and 50% soy milk kefir (G3) and the group treated with 100% soy milk kefir (G5). Combination of 50 % goat milk kefir and 50% soy milk kefir and administration of 100% soy milk kefir alone can control the level of IL-6 remained low in DM Wistar mice induced with streptozocin and nicotinamide. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=diabetes%20mellitus" title="diabetes mellitus">diabetes mellitus</a>, <a href="https://publications.waset.org/abstracts/search?q=goat%20milk%20kefir" title=" goat milk kefir"> goat milk kefir</a>, <a href="https://publications.waset.org/abstracts/search?q=soy%20milk%20kefir" title=" soy milk kefir"> soy milk kefir</a>, <a href="https://publications.waset.org/abstracts/search?q=interleukin%206" title=" interleukin 6"> interleukin 6</a> </p> <a href="https://publications.waset.org/abstracts/65540/effect-of-goat-milk-kefir-and-soy-milk-kefir-on-il-6-in-diabetes-mellitus-wistar-mice-models-induced-by-streptozotocin-and-nicotinamide" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/65540.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">285</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">504</span> Radioprotective Efficacy of Costus afer against the Radiation-Induced Hematology and Histopathology Damage in Mice</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Idowu%20R.%20Akomolafe">Idowu R. Akomolafe</a>, <a href="https://publications.waset.org/abstracts/search?q=Naven%20Chetty"> Naven Chetty</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: The widespread medical application of ionizing radiation has raised public concern about radiation exposure and, thus, associated cancer risk. The production of reactive oxygen species and free radicals as a result of radiation exposure can cause severe damage to deoxyribonucleic acid (DNA) of cells, thus leading to biological effect. Radiotherapy is an excellent modality in the treatment of cancerous cells, comes with a few challenges. A significant challenge is the exposure of healthy cells surrounding the tumour to radiation. The last few decades have witnessed lots of attention shifted to plants, herbs, and natural product as an alternative to synthetic compound for radioprotection. Thus, the study investigated the radioprotective efficacy of Costus afer against whole-body radiation-induced haematological, histopathological disorder in mice. Materials and Method: Fifty-four mice were randomly divided into nine groups. Animals were pretreated with the extract of Costus afer by oral gavage for six days before irradiation. Control: 6 mice received feed and water only; 6 mice received feed, water, and 3Gy; 6 mice received feed, water, and 6Gy; experimental: 6 mice received 250 mg/kg extract; 6 mice received 500 mg/kg extract; 6 mice received 250 mg/kg extract and 3Gy; 6 mice received 500 mg/kg extract and 3Gy; 6 mice received 250 mg/kg extract and 6Gy; 6 mice received 500 mg/kg extract and 6Gy in addition to feeding and water. The irradiation was done at the Radiotherapy and Oncology Department of Grey's Hospital using linear accelerator (LINAC). Thirty-six mice were sacrificed by cervical dislocation 48 hours after irradiation, and blood was collected for haematology tests. Also, the liver and kidney of the sacrificed mice were surgically removed for histopathology tests. The remaining eighteen (18) mice were used for mortality and survival studies. Data were analysed by one-way ANOVA, followed by Tukey's multiple comparison test. Results: Prior administration of Costus afer extract decreased the symptoms of radiation sickness and caused a significant delay in the mortality as demonstrated in the experimental mice. The first mortality was recorded on day-5 post irradiation, and this happened to the group E- that is, mice that received 6Gy but no extract. There was significant protection in the experimental mice, as demonstrated in the blood counts against hematopoietic and gastrointestinal damage when compared with the control. The protection was seen in the increase in blood counts of experimental animals and the number of survivor. The protection offered by Costus afer may be due to its ability to scavenge free radicals and restore gastrointestinal and bone marrow damage produced by radiation. Conclusions: The study has demonstrated that exposure of mice to radiation could cause modifications in the haematological and histopathological parameters of irradiated mice. However, the changes were relieved by the methanol extract of Costus afer, probably through its free radical scavenging and antioxidant properties. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=costus%20afer" title="costus afer">costus afer</a>, <a href="https://publications.waset.org/abstracts/search?q=hematological" title=" hematological"> hematological</a>, <a href="https://publications.waset.org/abstracts/search?q=mortality" title=" mortality"> mortality</a>, <a href="https://publications.waset.org/abstracts/search?q=radioprotection" title=" radioprotection"> radioprotection</a>, <a href="https://publications.waset.org/abstracts/search?q=radiotherapy" title=" radiotherapy"> radiotherapy</a> </p> <a href="https://publications.waset.org/abstracts/125484/radioprotective-efficacy-of-costus-afer-against-the-radiation-induced-hematology-and-histopathology-damage-in-mice" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/125484.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">140</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">503</span> Ameliorating Effects of Rosemary and Costus on Blood-Associated Toxicity in Ehrlich-Bearing Mice Treated with Cisplatin</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Yousry%20El-Sayed%20Elbolkiny">Yousry El-Sayed Elbolkiny</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohamed%20Labib%20%20Salem"> Mohamed Labib Salem</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: Rosemary (ROLE) and costus (SLRE) have been established to show antioxidant effects. Aim: This study aimed to evaluate the ameliorating effects of ROLE and SLRE on the side effects induced by cisplatin (CIS) in tumor-bearing mice. Materials and Methods: Extracts of ROLE and SLRE were examined for their phytochemical activities. To evaluate their anti-tumor effects, mice were inoculated intraperitoneally (i.p.) with 2.5x105 Ehrlich ascites carcinoma (EAC) and then treated i.p. with CIS at days 3-7 and with ROLE (dose) or SLRE (dose) at days 3-14. Mice were sacrificed on day 14 for CBC and T-cell analyses. Results: Phytochemical analysis revealed that both ROLE and SLRE showed similar antioxidant activities. Treatment of EAC-bearing mice with CIS-induced antitumor efficacy of about 90%. Treatment with CIS in combination with ROLE or SLRE did not further enhance the antitumor activity of CIS. However, co-administration of ROLE or SLRE with CIS significantly increased the antitumor efficacy of CIS. Flow cytometric analysis showed that the numbers of CD4+ and CD8+ T cells were decreased in EAC-bearing mice after treatment with CIS. Treatment with both ROLE and SLRE improved the number of these cells. Conclusion: Combinatorial treatment with rosemary and costus can enhance the antitumor activity of CIS <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=CBC" title="CBC">CBC</a>, <a href="https://publications.waset.org/abstracts/search?q=cisplantin" title=" cisplantin"> cisplantin</a>, <a href="https://publications.waset.org/abstracts/search?q=costus" title=" costus"> costus</a>, <a href="https://publications.waset.org/abstracts/search?q=rosemary" title=" rosemary"> rosemary</a> </p> <a href="https://publications.waset.org/abstracts/185377/ameliorating-effects-of-rosemary-and-costus-on-blood-associated-toxicity-in-ehrlich-bearing-mice-treated-with-cisplatin" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/185377.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">48</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">502</span> Pharmacodynamic Interaction between Tamsulosin and Finasteride Treatment on Induced Benign Prostate Hyperplasia in Mice by Using Chou-Talalay Method</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Firas%20Rashad%20Al-Samarai">Firas Rashad Al-Samarai</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Introduction: Benign prostatic hyperplasia (BPH) is a common condition as men get older. An enlarged prostate gland can cause uncomfortable urinary symptoms, such as blocking the flow of urine out of the bladder. It can also cause bladder, urinary tract, or kidney problems. Objective: to evaluate the efficacy and interaction of tamsulosin with finasteride treatment on induced benign prostate hyperplasia (BPH) in mice. Methods: BPH was induced by subcutaneous injection of testosterone propionate (20 mg/kg) for 30 days. Eighty-five mice were divided into five groups. The first group (G1): twenty-five mice induced BPH treated with tamsulosin orally and divided into five equal subgroups with doses (0.017, 0.052, 0.087, 0. 123, and 0.158) mg/kg, the second group (G2): twenty-five mice induced BPH treated with finasteride orally and divided into five equal subgroups with doses (0.175, 0.527, 0.878, 1.23, and 1.580) mg/kg. the third group (G3): twenty-five mice induced BPH treated with a combination of tamsulosin with finasteride orally, and divided into five equal subgroups with doses (0.0085, 0.0875), (0.026, 0.2635), (0.0435, 0.439) , (0.0615, 0.615) and ( 0.079 , 0.790 ) mg/kg respectively. Fourth group (G4): five mice induced BPH and treated distilled water. Fifth group (G5): five mice were not inducing BPH and without any treatment. Results: The results showed a gradual significant increase in prostate weight % and prostate index % Inhibitions until reached saturation in the last two doses of tamsulosin, finasteride, and combination groups, the maximum effective dose of tamsulosin and finasteride were (0.156) and (1.495) mg/kg respectively. Moreover, the effective dose of the combination (tamsulosin and finasteride) was estimated (0.06876, 0.6876) mg/kg, respectively, as well as the type of interaction was synergism and the value of the combination index was 0.046. Conclusions: We concluded that the combination of tamsulosin with finasteride showed a synergistic effect in BPH treatment by minimizing the side effect of each drug as s result of decreasing the dose of each one. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Tamsulosin" title="Tamsulosin">Tamsulosin</a>, <a href="https://publications.waset.org/abstracts/search?q=Finasteride" title=" Finasteride"> Finasteride</a>, <a href="https://publications.waset.org/abstracts/search?q=combination" title=" combination"> combination</a>, <a href="https://publications.waset.org/abstracts/search?q=BPH" title=" BPH"> BPH</a> </p> <a href="https://publications.waset.org/abstracts/164994/pharmacodynamic-interaction-between-tamsulosin-and-finasteride-treatment-on-induced-benign-prostate-hyperplasia-in-mice-by-using-chou-talalay-method" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/164994.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">76</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">501</span> Antihyperglycemic Effect of Aqueous Extract of Foeniculum vulgare Miller in Diabetic Mice</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Singh%20Baljinder">Singh Baljinder</a>, <a href="https://publications.waset.org/abstracts/search?q=Sharma%20Navneet"> Sharma Navneet</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Foeniculum vulgare Miller is a biennial medicinal and aromatic plant belonging to the family Apiaceae (Umbelliferaceae). It is a hardy, perennial–umbelliferous herb with yellow flowers and feathery leaves. The aim is to study the control of blood glucose in alloxan induced diabetic mice.Method used for extraction was continuous hot percolation method in which Soxhlet apparatus was used.95%ethanol was used as solvent. Male albino mice weighing about 20-25 g obtained from Guru Angad Dev University of Veterinary Science, Ludhiana were used for the study. Diabetes was induced by a single i.p. injection of 125 mg/kg of alloxan monohydrate in sterile saline (11). After 48 h, animals with serum glucose level above 200 mg/dl (diabetic) were selected for the study. Blood samples from mice were collected by retro-orbital puncture (ROP) technique. Serum glucose levels were determined by glucose oxidase and peroxidase method. Single administration (single dose) of aqueous extract of fennel (25, 50, and 100 mg/kg, p.o.) in diabetic Swiss albino mice, showed reduction in serum glucose level after 45 min. Maximum reduction in serum glucose level was seen at doses of 100 mg/kg. Aqueous extract of fennel in all doses except 25 mg/kg did not cause any significant decrease in blood glucose. It may be said that the aqueous extract of fennel decreased the serum glucose level and improved glucose tolerance owing to the presence of aldehyde moiety. The aqueous extract of fennel has antihyperglycemic activity as it lowers serum glucose level in diabetic mice. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Foeniculum%20vulgare%20Miller" title="Foeniculum vulgare Miller">Foeniculum vulgare Miller</a>, <a href="https://publications.waset.org/abstracts/search?q=antihyperglycemic" title=" antihyperglycemic"> antihyperglycemic</a>, <a href="https://publications.waset.org/abstracts/search?q=diabetic%20mice" title=" diabetic mice"> diabetic mice</a>, <a href="https://publications.waset.org/abstracts/search?q=Umbelliferaceae" title=" Umbelliferaceae "> Umbelliferaceae </a> </p> <a href="https://publications.waset.org/abstracts/9969/antihyperglycemic-effect-of-aqueous-extract-of-foeniculum-vulgare-miller-in-diabetic-mice" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/9969.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">286</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">500</span> Anethum graveolens Prevents Liver and Kidney Injury, Oxidative Stress and Inflammation in Mice Exposed to Nicotine Perinatally</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Saleh%20N.%20Maodaa">Saleh N. Maodaa</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Perinatal exposure to nicotine imbalances the redox status in newborns. This study investigated the effect of Anethum graveolens (dill) extract on oxidative stress and tissue injury in the liver and kidney of mice newborns exposed to nicotine perinatally. Pregnant mice received nicotine (0.25 mg/kg) on gestational day 12 to day 5 after birth and/or A. graveolens extract on a gestational day 1 to day 15 after birth. Newborn mice exposed to nicotine showed multiple histopathological alterations in the kidney and liver, including inflammatory cell infiltration and degenerative changes. Nicotine exposure increased hepatic and renal reactive oxygen species (ROS), lipid peroxidation, tumor necrosis factor (TNF-_), interleukin-6 (IL-6), and inducible nitric oxide synthase (iNOS) (p < 0.001), and decreased antioxidant defenses (p < 0.001). A. graveolens supplementation significantly prevented liver and kidney injury, suppressed ROS generation (p < 0.001), lipid peroxidation (p < 0.001), and inflammatory response (p < 0.001), and enhanced antioxidant defenses. In addition, A. graveolens upregulated hepatic and renal Nrf2 and HO-1 mRNA and increased HO-1 activity in normal and nicotine-exposed mice. In conclusion, A. graveolens protects against perinatal nicotine-induced oxidative stress, inflammation, and tissue injury in the liver and kidney of newborn mice. A. graveolens upregulated hepatic and renal Nrf2/HO-1 signaling and enhanced antioxidant defenses in mice. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=dill" title="dill">dill</a>, <a href="https://publications.waset.org/abstracts/search?q=oxidative%20stress" title=" oxidative stress"> oxidative stress</a>, <a href="https://publications.waset.org/abstracts/search?q=cytokines" title=" cytokines"> cytokines</a>, <a href="https://publications.waset.org/abstracts/search?q=nicotine" title=" nicotine"> nicotine</a> </p> <a href="https://publications.waset.org/abstracts/159904/anethum-graveolens-prevents-liver-and-kidney-injury-oxidative-stress-and-inflammation-in-mice-exposed-to-nicotine-perinatally" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/159904.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">80</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">499</span> Biocompatible Chitosan Nanoparticles as an Efficient Delivery Vehicle for Mycobacterium Tuberculosis Lipids to Induce Potent Cytokines and Antibody Response through Activation of γδ T-Cells in Mice</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ishani%20Das">Ishani Das</a>, <a href="https://publications.waset.org/abstracts/search?q=Avinash%20Padhi"> Avinash Padhi</a>, <a href="https://publications.waset.org/abstracts/search?q=Sitabja%20Mukherjee"> Sitabja Mukherjee</a>, <a href="https://publications.waset.org/abstracts/search?q=Santosh%20Kar"> Santosh Kar</a>, <a href="https://publications.waset.org/abstracts/search?q=Avinash%20Sonawane"> Avinash Sonawane</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Activation of cell mediated and humoral immune responses to Mycobacterium tuberculosis (Mtb) are critical for protection. Herein, we show that mice immunized with Mtb lipid bound chitosan nanoparticles(NPs) induce secretion of prominent Th1 and Th2 cytokines in lymph node and spleen cells, and also induced significantly higher levels of IgG, IgG1, IgG2 and IgM in comparison to control mice measured by ELISA. Furthermore, significantly enhanced γδ-T cell activation was observed in lymph node cells isolated from mice immunized with Mtb lipid coated chitosan-NPs as compared to mice immunized with chitosan-NPs alone or Mtb lipid liposomes through flow cytometric analysis. Also, it was observed that in comparison to CD8+ cells, significantly higher CD4+ cells were present in both the lymph node and spleen cells isolated from mice immunized with Mtb lipid coated chitosan NP. In conclusion, this study represents a promising new strategy for efficient delivery of Mtb lipids using chitosan NPs to trigger enhanced cell mediated and antibody response against Mtb lipids. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=antibody%20response" title="antibody response">antibody response</a>, <a href="https://publications.waset.org/abstracts/search?q=chitosan%20nanoparticles" title=" chitosan nanoparticles"> chitosan nanoparticles</a>, <a href="https://publications.waset.org/abstracts/search?q=cytokines" title=" cytokines"> cytokines</a>, <a href="https://publications.waset.org/abstracts/search?q=mycobacterium%20tuberculosis%20lipids" title=" mycobacterium tuberculosis lipids"> mycobacterium tuberculosis lipids</a> </p> <a href="https://publications.waset.org/abstracts/55795/biocompatible-chitosan-nanoparticles-as-an-efficient-delivery-vehicle-for-mycobacterium-tuberculosis-lipids-to-induce-potent-cytokines-and-antibody-response-through-activation-of-ghd-t-cells-in-mice" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/55795.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">280</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">498</span> The Effect of Dendrobium nobile Lindl. Alkaloids on the Blood Glucose and Amyloid Precursor Protein Metabolic Pathways in Db/Db Mice</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Juan%20Huang">Juan Huang</a>, <a href="https://publications.waset.org/abstracts/search?q=Nanqu%20Huang"> Nanqu Huang</a>, <a href="https://publications.waset.org/abstracts/search?q=Jingshan%20Shi"> Jingshan Shi</a>, <a href="https://publications.waset.org/abstracts/search?q=Yu%20Qiu"> Yu Qiu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Objectives: There are pathophysiological connections between type 2 diabetes mellitus (T2DM) and Alzheimer's disease (AD), and research on drugs with hypoglycemic and beta-amyloid (Aβ)-clearing effects have great therapeutic potential for AD. Dendrobium nobile Lindl. Alkaloids (DNLA) as one of the active compounds of Dendrobium nobile Lindl. In this study, we attempted to verify the hypoglycemic effect and investigate the effects of DNLA on the amyloid precursor protein (APP) metabolic pathway of the hippocampus in db/db mice. Methods: 4-weeks-old male C57BL/KsJ mice were the control group. And the same age and sexuality db/db mice were: model, DNLA-L (20 mg/kg), DNLA-M (40 mg/kg), and DNLA-H (80 mg/kg). After, mice were treated with different concentrations of DNLA for 17 weeks. The fasting blood glucose (FBG) was detected by glucose oxidase assay every week from the 4th to last week. The protein expression of β-amyloid 1-42 (Aβ1-42), β-site amyloid precursor protein-cleaving enzyme 1 (BACE1), and APP were examined by Western blotting. Results: The concentration of FBG and the protein expression of Aβ1-42, BACE1, and APP were increased in the hippocampus of the model group. Moreover, DNLA not only significantly decreased the concentration of FBG but also reduced the protein expressions of Aβ1-42, BACE1 and APP in the hippocampus of db/db mice in a dose-dependent manner. Conclusions: DNLA can decrease the protein expressions of Aβ1-42 in the hippocampus of db/db mice, and the mechanism may be involved in the APP metabolic pathway. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Alzheimer%27s%20disease" title="Alzheimer's disease">Alzheimer's disease</a>, <a href="https://publications.waset.org/abstracts/search?q=type%202%20diabetes%20mellitus" title=" type 2 diabetes mellitus"> type 2 diabetes mellitus</a>, <a href="https://publications.waset.org/abstracts/search?q=%CE%B2-site%20amyloid%20precursor%20protein-cleaving%20enzyme%201" title=" β-site amyloid precursor protein-cleaving enzyme 1"> β-site amyloid precursor protein-cleaving enzyme 1</a>, <a href="https://publications.waset.org/abstracts/search?q=traditional%20Chinese%20medicines" title=" traditional Chinese medicines"> traditional Chinese medicines</a>, <a href="https://publications.waset.org/abstracts/search?q=beta-amyloid" title=" beta-amyloid"> beta-amyloid</a> </p> <a href="https://publications.waset.org/abstracts/152548/the-effect-of-dendrobium-nobile-lindl-alkaloids-on-the-blood-glucose-and-amyloid-precursor-protein-metabolic-pathways-in-dbdb-mice" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/152548.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">250</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">497</span> Antidiabetic Evaluation of Pig (Sus scrofa) Bile on Alloxan-Induced BALB/c Mice</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=John%20Lyndon%20C.%20Lunnay">John Lyndon C. Lunnay</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This study discerns to evaluate the antidiabetic efficacy of pig bile on alloxan-induced BALB/c mice. The experimental animals were divided and selected using RCBD into 5 groups (n= 4): T1 (negative control), T2 (1ml/kg), T3 (2ml/kg), T4 (3ml/kg) and T5 (Glibenclamide). Hyperglycemia was induced by injecting 1% alloxan monohydrate intraperitoneally. A glucose tolerance test was performed using a 2g/kg glucose solution, and blood glucose levels were measured at different time intervals. 14 days of monitoring was also done to ensure effectivity and efficacy of the different treatments. Bodyweight was also determined. Results show that administration of treatments on test animals significantly reverted the blood glucose levels of mice in 60 minutes and 120 minutes using an oral glucose tolerance test. After 14 days of monitoring, normal blood glucose levels were seen significantly on T2 (1ml/kg), T3 (2ml/kg), T4 (3ml/kg), and T5 (Glibenclamide), which only suggests the efficacy of pig bile on lowering glucose levels on alloxan-induced diabetic mice. Bodyweight analysis shows no significant difference. Duncan’s multiple range test (DMRT) shows comparable efficacy and effectivity between T4 (3ml/kg) and T5 (Glibenclamide) on lowering BGL at different day and time intervals. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=pig%20bile" title="pig bile">pig bile</a>, <a href="https://publications.waset.org/abstracts/search?q=BALB%2Fc%20mice" title=" BALB/c mice"> BALB/c mice</a>, <a href="https://publications.waset.org/abstracts/search?q=blood%20glucose" title=" blood glucose"> blood glucose</a>, <a href="https://publications.waset.org/abstracts/search?q=Gllibenclamide" title=" Gllibenclamide"> Gllibenclamide</a> </p> <a href="https://publications.waset.org/abstracts/129960/antidiabetic-evaluation-of-pig-sus-scrofa-bile-on-alloxan-induced-balbc-mice" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/129960.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">148</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">496</span> Astragaioside IV Inhibits Type2 Allergic Contact Dermatitis in Mice and the Mechanism Through TLRs-NF-kB Pathway</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Xiao%20Wei">Xiao Wei</a>, <a href="https://publications.waset.org/abstracts/search?q=Dandan%20Sheng"> Dandan Sheng</a>, <a href="https://publications.waset.org/abstracts/search?q=Xiaoyan%20Jiang"> Xiaoyan Jiang</a>, <a href="https://publications.waset.org/abstracts/search?q=Lili%20Gui"> Lili Gui</a>, <a href="https://publications.waset.org/abstracts/search?q=Huizhu%20Wang"> Huizhu Wang</a>, <a href="https://publications.waset.org/abstracts/search?q=Xi%20Yu"> Xi Yu</a>, <a href="https://publications.waset.org/abstracts/search?q=Hailiang%20Liu"> Hailiang Liu</a>, <a href="https://publications.waset.org/abstracts/search?q=Min%20Hong"> Min Hong</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Objective: Mice Type2 allergic contact dermatitis was utilized in this study to explore the effect of AS-IV on Type 2 allergic inflammatory. Methods: The mice were topically sensitized on the shaved abdomens with 1.5% FITC solution on abdominal skin in the day 1 and day 2 and elicited on the right ear with 0.5% FITC solution at day 6. Mice were treated with either AS-IV or normal saline from day 1 to day 5 (induction phase). Auricle swelling was measured 24 h after the elicitation. Ear pathohistological examination was carried out by HE staining. IL-4\IL-13, and IL-9 levels of ear tissue were detected by ELISA. Mice were treated with AS-IV at the initial stage of induction phase, ear tissue was taked at day 3.TSLP level of ear tissue was detected by ELISA and TSLPmRNA\NF-kBmRNA\TLRs（TLR2\TLR3\TLR8\TLR9）mRNA were detected by PCR. Results: AS-IV induction phase evidently inhibited the auricle inflam-mation of the models; pathohistological results indicated that AS-IV induction phase alleviated local edema and angiectasis of mice models and reduced lymphocytic infiltration. AS-IV induction phase markedly decreased IL-4\IL-13, and IL-9 levels in ear tissue. Moreover, at the initial stage of induction pha-se, AS-IV significantly reduced TSLP\TSLPmRNA\NF-kBmRNA\TLR2mRNA\TLR8 mRNA levels in ear tissue. Conclusion: Administration with AS-IV in induction phase could inhibit Type 2 allergic contact dermatitis in mice significantly, and the mechanism may be related with regulating TSLP through TLRs-NF-kB pathway. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Astragaioside%20IV" title="Astragaioside IV">Astragaioside IV</a>, <a href="https://publications.waset.org/abstracts/search?q=allergic%20contact%20dermatitis" title=" allergic contact dermatitis"> allergic contact dermatitis</a>, <a href="https://publications.waset.org/abstracts/search?q=TSLP" title=" TSLP"> TSLP</a>, <a href="https://publications.waset.org/abstracts/search?q=interleukin-4" title=" interleukin-4"> interleukin-4</a>, <a href="https://publications.waset.org/abstracts/search?q=interleukin-13" title=" interleukin-13"> interleukin-13</a>, <a href="https://publications.waset.org/abstracts/search?q=interleukin-9" title=" interleukin-9"> interleukin-9</a> </p> <a href="https://publications.waset.org/abstracts/2925/astragaioside-iv-inhibits-type2-allergic-contact-dermatitis-in-mice-and-the-mechanism-through-tlrs-nf-kb-pathway" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/2925.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">431</span> </span> </div> </div> <ul class="pagination"> <li class="page-item disabled"><span class="page-link">&lsaquo;</span></li> <li class="page-item active"><span class="page-link">1</span></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=5XFAD%20mice&page=2">2</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=5XFAD%20mice&page=3">3</a></li> <li class="page-item"><a class="page-link" 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