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<!doctype html> <html> <head> <title>Isolation and Characterization of Indigenous Bacteria with Purifying Potential in Solid Palm Oil Extraction Sludge Generated by SOCAPALM-Mbambou , Frontiers in Environmental Microbiology, Science Publishing Group</title> <meta name="description" content="The oil palm industry contributes significantly to the economic development of producing countries such as Cameroon. Unfortunately, the exploitation of palm oil constitutes a source of environmental pollution due to the production of enormous quantities of waste during its extraction process, including solid sludge generating greenhouse gases which contribute to global warming climatic. All this leads to the search for alternatives which consists of isolating and characterizing indigenous bacteria with biodegradation capacities in sludge from palm oil extraction. The pH and bacterial counts were determined by the potentiometric method and the decimal dilution technique, respectively. The isolated bacteria were identified by their cultural, cellular and biochemical characteristics. In addition, the identification of Gram- bacteria was further explored by the API 20 E gallery. The palm oil biodegradability test was carried out on M2 medium supplemented with 2% palm oil. The solid sludge biodegradability test was carried out on liquid MSM medium supplemented with 4% sludge stock solution. The results showed that the sludge sample had a slightly alkaline pH of 7.3. A bacterial load of around 10<sup>9</sup> CFU/g of soil was counted. Thirty-one bacterial strains were isolated and purified, including 12 <i>Bacillus sp</i>, 10 <i>Pseudomonas sp</i>, 8 <i>Proteus mirabilis</i> and 1 <i>Klebsiella pneumoniae</i>. All isolates tested for their ability to degrade palm oil or solid sludge grew in culture media with palm oil or solid sludge as the sole source of carbon and energy but with a difference in load. Thus, isolates BI2, BI5, BI31, BI10 and BI 9 showed the highest degradation capacities. These isolates could be used to constitute consortia of microorganisms that can be used in the treatment of waste generated by palm oil extraction. "> <meta name="Keywords" content="Biodegradation, Consortium, Isolate, Microorganism, Solid Sludge"> <link rel="stylesheet" href="/js/bootstrap/css/bootstrap.min.css?v=20250116091759"> <meta name="dc.title" content="Isolation and Characterization of Indigenous Bacteria with Purifying Potential in Solid Palm Oil Extraction Sludge Generated by SOCAPALM-Mbambou "> <meta name="dc.creator" content="Tassong Saah Denis"><meta name="dc.creator" content="Simo Claude"><meta name="dc.creator" content="Taffouo Victor Désiré"> <meta name="dc.type" content="Research Article"> <meta name="dc.source" content="Frontiers in Environmental Microbiology 2024, Volume 10, Page 29"> <meta name="dc.date" content="2024-04-17"> <meta name="dc.identifier" content="10.11648/j.fem.20241002.11"> <meta name="dc.publisher" content="Science Publishing Group"> <meta name="dc.rights" content="2024 The Author(s)"> <meta name="dc.copyright" content="2024 The Author(s)"> <meta name="dc.rightsAgent" content="service@sciencepublishinggroup.com"> <meta name="dc.format" content="text/pdf"> <meta name="dc.language" content="En"> <meta name="dc.description" content="The oil palm industry contributes significantly to the economic development of producing countries such as Cameroon. Unfortunately, the exploitation of palm oil constitutes a source of environmental pollution due to the production of enormous quantities of waste during its extraction process, including solid sludge generating greenhouse gases which contribute to global warming climatic. All this leads to the search for alternatives which consists of isolating and characterizing indigenous bacteria with biodegradation capacities in sludge from palm oil extraction. The pH and bacterial counts were determined by the potentiometric method and the decimal dilution technique, respectively. The isolated bacteria were identified by their cultural, cellular and biochemical characteristics. In addition, the identification of Gram- bacteria was further explored by the API 20 E gallery. The palm oil biodegradability test was carried out on M2 medium supplemented with 2% palm oil. The solid sludge biodegradability test was carried out on liquid MSM medium supplemented with 4% sludge stock solution. The results showed that the sludge sample had a slightly alkaline pH of 7.3. A bacterial load of around 10<sup>9</sup> CFU/g of soil was counted. Thirty-one bacterial strains were isolated and purified, including 12 <i>Bacillus sp</i>, 10 <i>Pseudomonas sp</i>, 8 <i>Proteus mirabilis</i> and 1 <i>Klebsiella pneumoniae</i>. All isolates tested for their ability to degrade palm oil or solid sludge grew in culture media with palm oil or solid sludge as the sole source of carbon and energy but with a difference in load. Thus, isolates BI2, BI5, BI31, BI10 and BI 9 showed the highest degradation capacities. These isolates could be used to constitute consortia of microorganisms that can be used in the treatment of waste generated by palm oil extraction. "> <meta name="dc.subject" content="Biodegradation"><meta name="dc.subject" content="Consortium"><meta name="dc.subject" content="Isolate"><meta name="dc.subject" content="Microorganism"><meta name="dc.subject" content="Solid Sludge"> <meta name="prism.issn" content="2469-8067"> <meta name="prism.publicationName" content="Frontiers in Environmental Microbiology"> <meta name="prism.publicationDate" content="2024-04-17"> <meta name="prism.volume" content="10"> <meta name="prism.number" content="2"> <meta name="prism.section" content="Research Article"> <meta name="prism.startingPage" content="29"> <meta name="prism.endingPage" content="44"> <meta name="prism.copyright" content="2024 The Author(s)"> <meta name="prism.rightsAgent" content="service@sciencepublishinggroup.com"> <meta name="prism.url" content="https://www.sciencepg.com/article/10.11648/j.fem.20241002.11"> <meta name="prism.doi" content="doi:10.11648/j.fem.20241002.11"> <meta name="citation_issn" content="2469-8067"> <meta name="citation_journal_title" content="Frontiers in Environmental Microbiology"> <meta name="citation_journal_abbrev" content="Front. Environ. Microbiol."> <meta name="citation_publisher" content="Science Publishing Group"> <meta name="citation_title" content="Isolation and Characterization of Indigenous Bacteria with Purifying Potential in Solid Palm Oil Extraction Sludge Generated by SOCAPALM-Mbambou "> <meta name="citation_publication_date" content="2024/04"> <meta name="citation_online_date" content="2024/04/17"> <meta name="citation_volume" content="10"> <meta name="citation_issue" content="2"> <meta name="citation_firstpage" content="29"> <meta name="citation_lastpage" content="44"> <meta name="citation_article_type" content="Research Article"> <meta name="citation_fulltext_world_readable" content=""> <meta name="citation_language" content="En"> <meta name="citation_author" content="Tassong Saah Denis"> <meta name="citation_author_institution" content="Laboratory of Plant Biology, Department of Plant Biology, Faculty of Science, University of Douala, Douala, Cameroon"> <meta name="citation_author" content="Simo Claude"> <meta name="citation_author_institution" content="Laboratory of Plant Biology, Department of Plant Biology, Faculty of Science, University of Douala, Douala, Cameroon; Laboratory of Plant Physiology, Department of Biological Sciences, Higher Teacher’s Training College, University of Yaoundé I, Yaounde, Cameroon"> <meta name="citation_author" content="Taffouo Victor Désiré"> <meta name="citation_author_institution" content="Laboratory of Plant Biology, Department of Plant Biology, Faculty of Science, University of Douala, Douala, Cameroon"> <meta name="citation_doi" content="doi:10.11648/j.fem.20241002.11"> <meta name="citation_id" content="3841118"> <meta name="citation_pdf_url" content="http://article.sciencepg.com/pdf/j.fem.20241002.11"> <meta name="citation_reference" content="Jacquemard, J. 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Doctoral thesis in biotechnology, University of Oran, (2014), 153p."> <meta name="fulltext_pdf" content="http://article.sciencepg.com/pdf/j.fem.20241002.11"> <meta property="og:site_name" content="Science Publishing Group"> <meta property="og:type" content="article"> <meta property="og:url" content="https://www.sciencepg.com/article/10.11648/j.fem.20241002.11"> <meta property="og:title" content="Isolation and Characterization of Indigenous Bacteria with Purifying Potential in Solid Palm Oil Extraction Sludge Generated by SOCAPALM-Mbambou "> <meta property="og:description" content="The oil palm industry contributes significantly to the economic development of producing countries such as Cameroon. Unfortunately, the exploitation of palm oil constitutes a source of environmental pollution due to the production of enormous quantities of waste during its extraction process, including solid sludge generating greenhouse gases which contribute to global warming climatic. All this leads to the search for alternatives which consists of isolating and characterizing indigenous bacteria with biodegradation capacities in sludge from palm oil extraction. The pH and bacterial counts were determined by the potentiometric method and the decimal dilution technique, respectively. The isolated bacteria were identified by their cultural, cellular and biochemical characteristics. In addition, the identification of Gram- bacteria was further explored by the API 20 E gallery. The palm oil biodegradability test was carried out on M2 medium supplemented with 2% palm oil. The solid sludge biodegradability test was carried out on liquid MSM medium supplemented with 4% sludge stock solution. The results showed that the sludge sample had a slightly alkaline pH of 7.3. A bacterial load of around 10<sup>9</sup> CFU/g of soil was counted. Thirty-one bacterial strains were isolated and purified, including 12 <i>Bacillus sp</i>, 10 <i>Pseudomonas sp</i>, 8 <i>Proteus mirabilis</i> and 1 <i>Klebsiella pneumoniae</i>. All isolates tested for their ability to degrade palm oil or solid sludge grew in culture media with palm oil or solid sludge as the sole source of carbon and energy but with a difference in load. Thus, isolates BI2, BI5, BI31, BI10 and BI 9 showed the highest degradation capacities. These isolates could be used to constitute consortia of microorganisms that can be used in the treatment of waste generated by palm oil extraction. 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class="article_content_left left"> <input type="hidden" id="articleUniqueID" value="j.fem.20241002.11"> <div class="article_header"> <div class="article_header_top"> Research Article <span>|</span> <a href="/journal/384/open-access" target="_blank"><img src="/img/oa.png" class="oa"></a> <span>|</span> <a href="/journal/384/peer-review-at-sciencepg" style="color: #00599c; text-decoration: underline;" target="_blank">Peer-Reviewed</a> </div> <h3 class="ArticleTitle">Isolation and Characterization of Indigenous Bacteria with Purifying Potential in Solid Palm Oil Extraction Sludge Generated by SOCAPALM-Mbambou </h3> <div class="article_author"> <span class="author"> <a href="javascript:;" data-target="#author0" class="AuthorName" >Tassong Saah Denis</a><a href="http://orcid.org/0009-0008-0637-6793" target="_blank"><img src="/img/orcid_icon.png"></a>,  <div class="author_item person-info" id="author0" style="display: none;"> <p class="author_name">Tassong Saah Denis</p> <p class="Affiliation">Laboratory of Plant Biology, Department of Plant Biology, Faculty of Science, University of Douala, Douala, Cameroon</p> <p class="roles">Contributor Roles: Data curation, Funding acquisition, Investigation, Methodology, Software, Writing – original draft</p> <div class="contact_info"> <input type="hidden" class="person-id" value="10687509"> <p><img src="/img/email_icon.png"><a href="javascript:;" class="EmailAddress btn-email" data-toggle="modal" data-target="#emailModalScrollable">Contact Email</a></p> <p><img src="/img/orcid_icon.png"><a href="http://orcid.org/0009-0008-0637-6793" target="_blank" class="normal_link">http://orcid.org/0009-0008-0637-6793<i class="fas fa-external-link-alt"></i></a> </p> </div> </div> </span><span class="author"> <a href="javascript:;" data-target="#author1" class="AuthorName" >Simo Claude</a><sup>*</sup><a href="http://orcid.org/0009-0008-7800-4761" target="_blank"><img src="/img/orcid_icon.png"></a>,  <div class="author_item person-info" id="author1" style="display: none;"> <p class="author_name">Simo Claude</p> <p class="Affiliation">Laboratory of Plant Biology, Department of Plant Biology, Faculty of Science, University of Douala, Douala, Cameroon</p> <p class="Affiliation">Laboratory of Plant Physiology, Department of Biological Sciences, Higher Teacher’s Training College, University of Yaoundé I, Yaounde, Cameroon</p> <p class="roles">Contributor Roles: Conceptualization, Formal Analysis, Project administration, Software, Validation, Visualization, Writing – original draft, Writing – review & editing</p> <div class="contact_info"> <input type="hidden" class="person-id" value="10687510"> <p><img src="/img/email_icon.png"><a href="javascript:;" class="EmailAddress btn-email" data-toggle="modal" data-target="#emailModalScrollable">Contact Email</a></p> <p><img src="/img/orcid_icon.png"><a href="http://orcid.org/0009-0008-7800-4761" target="_blank" class="normal_link">http://orcid.org/0009-0008-7800-4761<i class="fas fa-external-link-alt"></i></a> </p> </div> </div> </span><span class="author"> <a href="javascript:;" data-target="#author2" class="AuthorName" >Taffouo Victor Désiré</a><a href="http://orcid.org/0000-0002-3745-6875" target="_blank"><img src="/img/orcid_icon.png"></a> <div class="author_item person-info" id="author2" style="display: none;"> <p class="author_name">Taffouo Victor Désiré</p> <p class="Affiliation">Laboratory of Plant Biology, Department of Plant Biology, Faculty of Science, University of Douala, Douala, Cameroon</p> <p class="roles">Contributor Roles: Supervision, Validation</p> <div class="contact_info"> <input type="hidden" class="person-id" value="10687511"> <p><img src="/img/email_icon.png"><a href="javascript:;" class="EmailAddress btn-email" data-toggle="modal" data-target="#emailModalScrollable">Contact Email</a></p> <p><img src="/img/orcid_icon.png"><a href="http://orcid.org/0000-0002-3745-6875" target="_blank" class="normal_link">http://orcid.org/0000-0002-3745-6875<i class="fas fa-external-link-alt"></i></a> </p> </div> </div> </span> </div> <div class="published"> <span>Published in </span> <a href="/journal/384/home" target="_blank"><i>Frontiers in Environmental Microbiology</i></a> (<a href="/journal/384/archive/3841002" target="_blank">Volume 10, Issue 2</a>) </div> <div class="article_time"> <span>Received: </span>7 March 2024     <span>Accepted: </span>25 March 2024     <span>Published: </span>17 April 2024 </div> <div class="vd"> <input type="hidden" id="downloadTotalizationUrl" value="https://w.sciencepublishinggroup.com/"> <span>Views:</span> <span class="spanViews"></span>      <span>Downloads:</span> <span class="spanDownloads"></span> </div> <div class="operation clearfix"> <div class="view_more left"> <a href="https://article.sciencepublishinggroup.com/pdf/j.fem.20241002.11" target="_blank" onclick="downLoadArticle(10087896, "https:\/\/w.sciencepublishinggroup.com\/", '.spanDownloads', "https:\/\/article.sciencepublishinggroup.com\/", "pdf\/j.fem.20241002.11", true)" > <i class="fas fa-file-pdf"></i>Download PDF </a> </div>  <div class="add_ope share_btn left"> <a href="javascript:;" id="toggleButton"> <img src="/img/share_icon.png">Share This Article </a> <div class="share_item toggle-div" id="myDiv"> <div class="s-popup__arrow"></div> <div class="share_list"> <ul> <li> <a id="twitterUrl" target="_blank"><img src="/img/twitter_icon.png">Twitter</a> </li> <li> <a id="linkedInUrl" target="_blank"><img src="/img/LinkedIn_icon.png">Linked In</a> </li> <li> <a id="facebookUrl" target="_blank"><img src="/img/facebook_icon.png">Facebook</a> </li> </ul> <script type="text/javascript"> var twitterUrl = "https://twitter.com/intent/tweet?text=" + twitterUrlEncode("Isolation and Characterization of Indigenous Bacteria with Purifying Potential in Solid Palm Oil Extraction Sludge Generated by SOCAPALM-Mbambou\r\n"); twitterUrl += "&hashtags=" + twitterUrlEncode("Science Publishing Group"); twitterUrl += "&url=" + twitterUrlEncode(location.origin + "/" + "article\/10.11648\/j.fem.20241002.11"); var linkedInUrl = "http://www.linkedin.com/shareArticle?mini=true&title=" + encodeURIComponent("Isolation and Characterization of Indigenous Bacteria with Purifying Potential in Solid Palm Oil Extraction Sludge Generated by SOCAPALM-Mbambou\r\n"); linkedInUrl += encodeURIComponent("&source=" + location.origin + "&summary=" + getMoreContentShow("The oil palm industry contributes significantly to the economic development of producing countries such as Cameroon. Unfortunately, the exploitation of palm oil constitutes a source of environmental pollution due to the production of enormous quantities of waste during its extraction process, including solid sludge generating greenhouse gases which contribute to global warming climatic. All this leads to the search for alternatives which consists of isolating and characterizing indigenous bacteria with biodegradation capacities in sludge from palm oil extraction. The pH and bacterial counts were determined by the potentiometric method and the decimal dilution technique, respectively. The isolated bacteria were identified by their cultural, cellular and biochemical characteristics. In addition, the identification of Gram- bacteria was further explored by the API 20 E gallery. The palm oil biodegradability test was carried out on M2 medium supplemented with 2% palm oil. The solid sludge biodegradability test was carried out on liquid MSM medium supplemented with 4% sludge stock solution. The results showed that the sludge sample had a slightly alkaline pH of 7.3. A bacterial load of around 10<sup>9<\/sup> CFU\/g of soil was counted. Thirty-one bacterial strains were isolated and purified, including 12 <i>Bacillus sp<\/i>, 10 <i>Pseudomonas sp<\/i>, 8 <i>Proteus mirabilis<\/i> and 1 <i>Klebsiella pneumoniae<\/i>. All isolates tested for their ability to degrade palm oil or solid sludge grew in culture media with palm oil or solid sludge as the sole source of carbon and energy but with a difference in load. Thus, isolates BI2, BI5, BI31, BI10 and BI 9 showed the highest degradation capacities. These isolates could be used to constitute consortia of microorganisms that can be used in the treatment of waste generated by palm oil extraction.\r\n", 200)) linkedInUrl += "&url=" + encodeURIComponent(location.origin + "/" + "article\/10.11648\/j.fem.20241002.11"); var facebookUrl = "https://www.facebook.com/sharer.php?u=" + location.origin + "/" + "article\/10.11648\/j.fem.20241002.11"; </script> </div> </div> </div> </div> </div> <div class="article_body"> <div class="section" id="abstract"> <div class="Abatract">Abstract</div> <p class="AbatractContent">The oil palm industry contributes significantly to the economic development of producing countries such as Cameroon. Unfortunately, the exploitation of palm oil constitutes a source of environmental pollution due to the production of enormous quantities of waste during its extraction process, including solid sludge generating greenhouse gases which contribute to global warming climatic. All this leads to the search for alternatives which consists of isolating and characterizing indigenous bacteria with biodegradation capacities in sludge from palm oil extraction. The pH and bacterial counts were determined by the potentiometric method and the decimal dilution technique, respectively. The isolated bacteria were identified by their cultural, cellular and biochemical characteristics. In addition, the identification of Gram- bacteria was further explored by the API 20 E gallery. The palm oil biodegradability test was carried out on M2 medium supplemented with 2% palm oil. The solid sludge biodegradability test was carried out on liquid MSM medium supplemented with 4% sludge stock solution. The results showed that the sludge sample had a slightly alkaline pH of 7.3. A bacterial load of around 10<sup>9</sup> CFU/g of soil was counted. Thirty-one bacterial strains were isolated and purified, including 12 <i>Bacillus sp</i>, 10 <i>Pseudomonas sp</i>, 8 <i>Proteus mirabilis</i> and 1 <i>Klebsiella pneumoniae</i>. All isolates tested for their ability to degrade palm oil or solid sludge grew in culture media with palm oil or solid sludge as the sole source of carbon and energy but with a difference in load. Thus, isolates BI2, BI5, BI31, BI10 and BI 9 showed the highest degradation capacities. These isolates could be used to constitute consortia of microorganisms that can be used in the treatment of waste generated by palm oil extraction. </p> </div> <div class="article_basic_info"> <table> <tr> <td> <span>Published in</span> </td> <td> <a href="/journal/384/home" target="_blank"><i>Frontiers in Environmental Microbiology</i></a> (<a href="/journal/384/archive/3841002" target="_blank">Volume 10, Issue 2</a>) </td> </tr> <tr> <td> <span>DOI</span> </td> <td> <a href="https://doi.org/10.11648/j.fem.20241002.11" target="_blank">10.11648/j.fem.20241002.11</a> </td> </tr> <tr> <td> <span>Page(s)</span> </td> <td>29-44</td> </tr> <tr> <td> <span>Creative Commons</span> </td> <td> <p class="basic_copyright"><img src="/img/copyright_icon2.png"></p> <p>This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (<a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a>), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. </p> </td> </tr> <tr> <td> <span>Copyright</span> </td> <td> <p>Copyright © The Author(s), 2024. Published by Science Publishing Group</p> </td> </tr> </table> </div> <div class="pre_next_article clearfix"> <div class="pre"> <a href="/article/10.11648/j.fem.20241001.13"><i class="fas fa-chevron-circle-left"></i><span>Previous article</span></a> </div> <div class="next"> <a href="/article/10.11648/j.fem.20241002.12"><span>Next article</span><i class="fas fa-chevron-circle-right"></i></a> </div> </div> <div class="section" id="keywords"> <div class="Keywords">Keywords</div> <p class="KeywordsContent">Biodegradation, Consortium, Isolate, Microorganism, Solid Sludge</p> </div> <div class="section" id="section1"><div class="Heading1">1. Introduction</div><div class="Text">The oil palm (<i>Elaeis guineensis</i>) is a perennial monocot plant of the <i>Arecaceae</i> family, native to West Africa which is mainly cultivated for its palm oil extracted from the pericarp of the fruit <div class="references_link" data-target="#references1"><div class="references_detail" style="display: none;" id="references1"><div class="references"><table class="normal_table"><tbody><tr><td>[1]</td><td>Jacquemard, J. C The oil palm. Quae Editions; (2012), 274 p.</td></tr></tbody></table></div></div>[1]</div>. Palm oil plays a key role in meeting global food needs and contributes significantly to the economic development of producing countries. In Cameroon, a production of 465,000 t was recorded during the 2021-2022 harvest campaign, placing it third in Africa and twelfth in the world behind Nigeria (1,400,000 t) and Ivory Coast (600,000 t) <div class="references_link" data-target="#references2"><div class="references_detail" style="display: none;" id="references2"><div class="references"><table class="normal_table"><tbody><tr><td>[2]</td><td>USDA. World palm oil production by country. (2022), 37p. <p class="doi_link"><a href="http://www.worldagriculturalproduction.com" target="_blank" class="normal_link">http://www.worldagriculturalproduction.com<i class="fas fa-external-link-alt"></i></a></p><div class="view_article"><a href="http://www.worldagriculturalproduction.com" target="_blank" class="normal_link">View Article<i class="fas fa-external-link-alt"></i></a></div></td></tr></tbody></table></div></div>[2]</div>. The multiplication of uses of palm oil (cosmetics, energy, biofuels, agri-food, etc.) has increased its demand. Currently, overall demand exceeds supply, and this trend is likely to strengthen in the future, making it a particularly attractive product for investors. According to forecasts from the OECD and the FAO, global palm oil production is expected to increase by 20% between 2021 and 2030. Supply therefore still remains largely in deficit compared to strong demand <div class="references_link" data-target="#references3"><div class="references_detail" style="display: none;" id="references3"><div class="references"><table class="normal_table"><tbody><tr><td>[3]</td><td>OCDE/FAO Perspectives agricoles de l’OCDE et de la FAO 2021-2030. Edition OCDE, paris; (2021), 4p. <p class="doi_link"><a href="https://doi.org/10.1787/e32bf104-fr" target="_blank" class="normal_link">https://doi.org/10.1787/e32bf104-fr<i class="fas fa-external-link-alt"></i></a></p><div class="view_article"><a href="https://doi.org/10.1787/e32bf104-fr" target="_blank" class="normal_link">View Article<i class="fas fa-external-link-alt"></i></a></div></td></tr></tbody></table></div></div>[3]</div>. According to FAO, 21 million hectares of forest in the world in general and 170,169 ha in Cameroon have been destroyed in favor of palm oil plantations <div class="references_link" data-target="#references4"><div class="references_detail" style="display: none;" id="references4"><div class="references"><table class="normal_table"><tbody><tr><td>[4]</td><td>FAO Market Assesment: Oil Crops, Oil and Meals; (2017), 18p.</td></tr></tbody></table></div></div>[4]</div>. As a result, forest clearing not only destroys biodiversity, but also endangers existing microflora and fauna. In addition, the slow decomposition and fires of peatlands, resulting from the expansion of oil palm plantations, have caused a significant loss of biodiversity as well as high greenhouse gas emissions <div class="references_link" data-target="#references5"><div class="references_detail" style="display: none;" id="references5"><div class="references"><table class="normal_table"><tbody><tr><td>[5]</td><td>FAO International Day of Forests. Indonesia’s Forests in the Limelight | FAO in Indonesia | Food and Agriculture Organization of the United Nations; (2015), 56p. Disponible sur: <p class="doi_link"><a href="http://www.fao.org/indonesia/news/detail-events/en/c/409648/" target="_blank" class="normal_link">http://www.fao.org/indonesia/news/detail-events/en/c/409648/<i class="fas fa-external-link-alt"></i></a></p><div class="view_article"><a href="http://www.fao.org/indonesia/news/detail-events/en/c/409648/" target="_blank" class="normal_link">View Article<i class="fas fa-external-link-alt"></i></a></div></td></tr></tbody></table></div></div>[5]</div>. The cultivation policy implemented by oil growers in Cameroon as in all producing countries aims to increase production yield in order to satisfy high demand, which leads them to use enormous quantities of chemical fertilizers in their practices fertilization. According to <div class="references_link" data-target="#references6"><div class="references_detail" style="display: none;" id="references6"><div class="references"><table class="normal_table"><tbody><tr><td>[6]</td><td>Halimah, M., Zulkifli H., Subramaniam V., Tan Y. A., Puah C. W., Chong C., Choo Y. M. Life cycle assessment of oil palm seedling production (Part 1). Journal of Oil Palm Research. (2010), 22(3), 878-886.</td></tr></tbody></table></div></div>[6]</div>, the application of nitrogen fertilizers is the primary source of greenhouse gas emissions in plantations. In addition, some of these fertilizers are responsible for the pollution of surface water and groundwater. Hence the need to find non-polluting alternatives in fertilization practices. In this context, replacing mineral fertilizers with organic fertilizers, such as compost, could provide a solution to this problem <div class="references_link" data-target="#references7"><div class="references_detail" style="display: none;" id="references7"><div class="references"><table class="normal_table"><tbody><tr><td>[7]</td><td>Pellerin, S., Bamière, L., Pardon, L. Agriculture and greenhouse gases: Ten actions to reduce emissions. Quae Editions; 2015. 202 p.</td></tr></tbody></table></div></div>[7]</div>. Although the oil palm industry is recognized for its contribution to economic growth and rapid development in Cameroon, it also contributes to environmental pollution due to the production of huge quantities of waste during the process extraction. These wastes are mainly liquid discharges forming a final effluent called POME (Palm Oil Mill Effluent), stalks or EFB (Empty Fruit Bunch), solid sludge from three-phase centrifugation, fibers and hulls <div class="references_link" data-target="#references8"><div class="references_detail" style="display: none;" id="references8"><div class="references"><table class="normal_table"><tbody><tr><td>[8]</td><td>Parveen, F. R., Rajeev, P. S., Hakimi, I. M., Norizan E. Review of Current Palm Oil Mill Effluent (POME) Treatment Methods: Vermicomposting as a Sustainable Practice. World Applied Sciences Journal. (2010), 10(10), 1190-1201.</td></tr></tbody></table></div></div>[8]</div>. Managing this waste poses enormous problems for agro-industries. Some of this waste, such as fibers and hulls, is regularly used for the production of energy in boilers. In most industries, liquid effluents are treated in a lagoon system before discharge into waterways, but this treatment is not very effective due to the high content of the clarification sludge which quickly loads into the water. the settling basin and contaminate the other basins, hence the high levels of biochemical oxygen demand (BOD), chemical oxygen demand (COD) and suspended matter (SM). Therefore, it is necessary to regularly clean the sludge from the settling basins and treat it otherwise. The method of managing stalks as well as solid sludge practiced by agro-industries in Cameroon is their dumping in the field for their natural composting. However, this practice causes environmental pollution and deteriorates the surrounding environment. Solid sludge and cobs take several years to degrade naturally when dumped in the field. In contact with plants, palm oil sludge inhibits their growth and causes their death <div class="references_link" data-target="#references9"><div class="references_detail" style="display: none;" id="references9"><div class="references"><table class="normal_table"><tbody><tr><td>[8]</td><td>Parveen, F. R., Rajeev, P. S., Hakimi, I. M., Norizan E. Review of Current Palm Oil Mill Effluent (POME) Treatment Methods: Vermicomposting as a Sustainable Practice. World Applied Sciences Journal. (2010), 10(10), 1190-1201.</td></tr></tbody></table></div></div>[8]</div>. In addition, the leaching of sludge by rainwater causes water depletion and leads to aquatic pollution. Furthermore, solid sludge is responsible for bad odors and therefore a source of diseases. Given the abundance of this sludge generated by palm oil agro-industries in Cameroon and their impact on environmental pollution, there is an urgent need to put in place an effective management system for their treatment, in order to preserve the environment. With this in mind, biological treatment by composting can be a good option for the sustainable management of this waste. Composting would make it possible to treat this waste rich in organic matter and the opportunity to use it in the field as organic fertilizer. This treatment system would significantly reduce the use of chemical fertilizers and the environmental impact of oil palm plantations. Indeed, this method relies on the ability of bacteria to use fatty acids as a source of carbon in their metabolism. The method requires competent bacteria to be available for its implementation. To do this, the principle is to isolate this type of bacteria in soils polluted by fatty acids. However, Cameroonian companies operating in the field of industrial waste treatment, particularly biological treatment, always use laboratories in Western or Asian countries to obtain microorganisms suitable for biodegradation, which is very expensive and therefore makes the cost very high waste processing for local businesses. However, starting from the hypothesis according to which, for each type of waste accumulated in the environment, certain microorganisms can adapt to live there and use it as a source of carbon and therefore can degrade this waste, it is therefore possible to isolate these indigenous microorganisms and use them in the biodegradation process. These microorganisms could be used to constitute consortia of microorganisms that can be used in the treatment of waste generated by palm oil extraction.</div></div><div class="section" id="section2"><div class="Heading1">2. Materials and Methods</div><div class="Text" id="section2_1"><p class='Heading2'>2.1. Materials</p></div><div class="Text">The palm oil sludge samples used in this study were taken from the Cameroonian palm grove company<a class='a_link' href='javascript:;'> </a>(SOCAPALM-Mbambou) site located in Dizangue-Cameroun in the Littoral region, Sanaga maritime Division, Dizangue Sub-division.<a class='a_link' href='javascript:;'>.</a> The palm oil used as substrate was purchased on the local market. This oil was then sterilized in an autoclave before being used.</div><div class="Text" id="section2_2"><p class='Heading2'>2.2. Methods</p></div><div class="Text" id="section2_2_1"><p class='Heading3'>2.2.1. Evaluation of Hydrogen Potential</p></div><div class="Text">The pH of the sample was measured using a “SARTORIUS AGCOTTINGEN” type pH meter. A suspension of solid sludge was prepared in distilled water at 5/50 (mass/volume ratio). Stirring was carried out for a period of one hour followed by decantation, then the probe was immersed in the solution and the value was recorded.</div><div class="Text" id="section2_2_2"><p class='Heading3'>2.2.2. Isolation, Purification and Storage of Bacterial Isolates</p></div><div class="Text">The serial decimal dilution technique was used to enumerate total mesophilic aerobic microflora in sludge samples <div class="references_link" data-target="#references10"><div class="references_detail" style="display: none;" id="references10"><div class="references"><table class="normal_table"><tbody><tr><td>[9]</td><td>Austin, B., Bucke, D., Feist, S. W., Helm, M. M. Disease problems among cultured bivalve larvae. ministry of Agricuture, Fisheries and Food, Directorate of Fisheries Research, Lowestoft, InternaI Report N°16; (1988), p. 22.</td></tr><tr><td>[10]</td><td>Goma-Tchimbakala, J., Obambi, N. J. R, Lebonguy, A. A., Gouolally, T. Biodegradation of PAHs from Used Oil by Consortia of Microorganisms Isolated from Polluted Soils of Brazzaville, Congo. European Journal of Scientific Research. (2020), 155(4), 378–387.</td></tr></tbody></table></div></div>[9, 10]</div>. The dilution series ranging from 10<sup>-1</sup> (stock solution) to 10<sup>-8 </sup>was prepared from a suspension of 10g of sludge in 90 mL of sterile distilled water. Subsequently, 0.1 mL of each dilution was plated in two repetitions on the Petri dishes containing ordinary nutrient agar (NA). The boxes were incubated at 37°C for 24 hours. At the end of the incubation, the colonies presenting morphological differences were counted in CFU (Colony Forming Unit) and the number of germs per gram of the sample was determined according to the formula of Marchal and Bourdon (1982) <div class="references_link" data-target="#references11"><div class="references_detail" style="display: none;" id="references11"><div class="references"><table class="normal_table"><tbody><tr><td>[11]</td><td>Marchal, N., Bourdon J. L. Culture media for the isolation and biochemical identification of bacteria. Doin Ed., Paris; (1982), 482p.</td></tr></tbody></table></div></div>[11]</div>:</div><div class="Text">N = n / d.v</div><div class="Text">where N: number of microorganisms in CFU/mL; n: number of colonies counted; v: Volume collected (0.1mL); d: Dilution.</div><div class="Text">The colonies counted were purified by successive and alternating subcultures in liquid medium, then in solid medium <div class="references_link" data-target="#references12"><div class="references_detail" style="display: none;" id="references12"><div class="references"><table class="normal_table"><tbody><tr><td>[12]</td><td>Delarras, C., Practical Microbiology For The Sanitair Analysis Or Control Laboratory, 1st edition, Paris: Tec Edition and doc; (2007), p. 476.</td></tr></tbody></table></div></div>[12]</div>. After purification, the isolates were stored in Eppendorf tubes at 4°C on solid medium (NA) and at 20°C in nutrient broth supplemented with glycerol (1:1, v/v) <div class="references_link" data-target="#references13"><div class="references_detail" style="display: none;" id="references13"><div class="references"><table class="normal_table"><tbody><tr><td>[13]</td><td>Obayori, O. S., Ilori, M. O., Adebusoye, S. A., Oyetibo, G. O., Amund, O. O. Degradation of hydrocarbons and biosurfactant production by Pseudomonas sp strain LP1. World. J. Microbiol. Biotechnol, (2009), 1615-1623.</td></tr></tbody></table></div></div>[13]</div>.</div><div class="Text" id="section2_2_3"><p class='Heading3'>2.2.3. Cultural and Cellular Characterization of Isolates</p></div><div class="Text" id="section(i)"><p class='Heading2'>(i). Macroscopic Appearance</p></div><div class="Text">Observing the macroscopic appearance of the colonies allows an initial characterization to be carried out. According to Joffin and Leyral (2006), the macroscopic identification elements are as follows: shape, color, elevation, contours, size, opacity, consistency and surface <div class="references_link" data-target="#references14"><div class="references_detail" style="display: none;" id="references14"><div class="references"><table class="normal_table"><tbody><tr><td>[14]</td><td>Joffin, J. N., Leyral, G. Technical microbiology, Volume 1: Dictionary of techniques, 4th edition. CRDP edition of aquitaire; (2006), p. 368.</td></tr></tbody></table></div></div>[14]</div>. Mossel medium supplemented with polymixin B (1 mg/mL), a medium specific to the growth of <i>Bicillus</i>, was used to verify the capacity of bacteria suspected to belong to the genus <i>Bacillus sp</i>., according to the modified method of Goma-Tchimbakala <i>et al.</i> (2020) <div class="references_link" data-target="#references15"><div class="references_detail" style="display: none;" id="references15"><div class="references"><table class="normal_table"><tbody><tr><td>[10]</td><td>Goma-Tchimbakala, J., Obambi, N. J. R, Lebonguy, A. A., Gouolally, T. Biodegradation of PAHs from Used Oil by Consortia of Microorganisms Isolated from Polluted Soils of Brazzaville, Congo. European Journal of Scientific Research. (2020), 155(4), 378–387.</td></tr></tbody></table></div></div>[10]</div>.</div><div class="Text" id="section(ii)"><p class='Heading2'>(ii). Microscopic Appearance</p></div><div class="Text">An observation under a microscope of the bacteria in the fresh state made it possible to see the mobility. The shape and pattern of grouping were determined by methylene blue staining. A Gram stain was done to classify the bacteria according to bacterial type and confirm their purity in addition to Malachite green staining to see the presence of resistance spores <div class="references_link" data-target="#references16"><div class="references_detail" style="display: none;" id="references16"><div class="references"><table class="normal_table"><tbody><tr><td>[15]</td><td>Selmoun, M., Benkhebcheche, D. E. Contribution to the study of the biodegradation of hydrocarbons in drilling muds by bacteria producing biosurfactants. Document of the end of Master cycle for obtaining a Master’s degree in Microbial Ecology; (2016), 77p.</td></tr></tbody></table></div></div>[15]</div>.</div><div class="Text" id="section2_2_4"><p class='Heading3'>2.2.4. Biochemical Characterization of Isolates</p></div><div class="Text"><i>Orientation Test: Potash Test (3% Aqueous KOH Solution)</i></div><div class="Text">The potash test was carried out in addition to the classic Gram staining method. The KOH test was carried out according to the method described by Carlone <i>et al. </i>(1983) taken up by Selmoun <i>et al.</i> (2016) <div class="references_link" data-target="#references17"><div class="references_detail" style="display: none;" id="references17"><div class="references"><table class="normal_table"><tbody><tr><td>[16]</td><td>Carlone, GM et al. Methods for Distinguishing Gram-Positive from Gram-Negative Bacteria. J Clin Microbiol. (1983), 1157-1159.</td></tr><tr><td>[15]</td><td>Selmoun, M., Benkhebcheche, D. E. Contribution to the study of the biodegradation of hydrocarbons in drilling muds by bacteria producing biosurfactants. Document of the end of Master cycle for obtaining a Master’s degree in Microbial Ecology; (2016), 77p.</td></tr></tbody></table></div></div>[16, 15]</div>. On a glass slide, a drop of 3% potassium hydroxide was pipetted and then a visible loop of cells from a single, well-isolated colony was mixed into the drop. When the mixture becomes viscous and stringy within 60 seconds after mixing (KOH positive), the colony is considered Gram negative.</div><div class="Text">(a). Characterization of Gram+ Bacteria</div><div class="Text"><i>Search for oxidase</i></div><div class="Text">The oxidase test was carried out according to the method of <div class="references_link" data-target="#references18"><div class="references_detail" style="display: none;" id="references18"><div class="references"><table class="normal_table"><tbody><tr><td>[17]</td><td>Singleton, P. Bacteriology, Duonod Edition, 4th edition Paris; (1999), p. 415.</td></tr></tbody></table></div></div>[17]</div>. An oxidase disk previously soaked with a drop of sterile distilled water was placed on a slide and brought into contact with a freshly cultured bacterial colony. The appearance of a purple color directly obtained indicates that the test is positive.</div><div class="Text"><i>Search for catalase</i></div><div class="Text">Using a platinum loop, a drop of the bacterial suspension is placed on a glass slide then emulsified in a drop of 10 volume hydrogen peroxide. The presence of catalase is immediately manifested by gas bubbles (effervescence) corresponding to the released oxygen <div class="references_link" data-target="#references19"><div class="references_detail" style="display: none;" id="references19"><div class="references"><table class="normal_table"><tbody><tr><td>[11]</td><td>Marchal, N., Bourdon J. L. Culture media for the isolation and biochemical identification of bacteria. Doin Ed., Paris; (1982), 482p.</td></tr></tbody></table></div></div>[11]</div>.</div><div class="Text">(b). Characterization of Gram- bacteria</div><div class="Text">In addition to the search for respiratory enzymes such as catalase and oxidase, the other biochemical characteristics of the Gram- bacterial isolates were determined using an API 20E identification gallery (BioMérieux). Seeding of the galleries was carried out from 24-hour colonies following the protocol proposed by the manufacturer. The galleries were incubated at 37°C for 48 h. The reactions produced during incubation are reflected by the change in the colored indicator. The results were noted on the result sheet provided by the manufacturer and the identification was made using the APIWEB identification program accessible online.</div><div class="Text" id="section2_2_5"><p class='Heading3'>2.2.5. Biodegradability Test of Palm Oil and Extraction Sludge Using Isolates</p></div><div class="Text" id="section(i)"><p class='Heading2'>(i). Palm oil Biodegradability Test</p></div><div class="Text">M2 medium supplemented with 2% palm oil was used for this test. Two agar plates were provided for each strain: one without yeast extract and the other with 1g/L of yeast extract in order to make a comparison and confirm the degradation capacity of palm oil as the sole source of carbon and energy by these bacteria. This test was followed for five days. The degradation efficiency of the isolates was determined by comparing the growth speed and colony load <div class="references_link" data-target="#references20"><div class="references_detail" style="display: none;" id="references20"><div class="references"><table class="normal_table"><tbody><tr><td>[15]</td><td>Selmoun, M., Benkhebcheche, D. E. Contribution to the study of the biodegradation of hydrocarbons in drilling muds by bacteria producing biosurfactants. Document of the end of Master cycle for obtaining a Master’s degree in Microbial Ecology; (2016), 77p.</td></tr></tbody></table></div></div>[15]</div>.</div><div class="Text" id="section(ii)"><p class='Heading2'>(ii). Biodegradability Test of Solid Sludge from Palm Oil Extraction</p></div><div class="Text">This test was carried out by monitoring the growth kinetics of the bacteria according to the method of <div class="references_link" data-target="#references21"><div class="references_detail" style="display: none;" id="references21"><div class="references"><table class="normal_table"><tbody><tr><td>[18]</td><td>Ndzobo, N. E. J., Tavea, M. F., Bella, J et al. Isolation, Screening and Biochemical Identification of Bacteria with Purifying Potential in the Effluents of a Paint Manufacturing Industry. Frontiers in Environmental Microbiology. 8(1), (2022), pp. 6-12. <p class="doi_link"><a href="https://doi.org/10.11648/j.fem.20220801.12" target="_blank" class="normal_link">https://doi.org/10.11648/j.fem.20220801.12<i class="fas fa-external-link-alt"></i></a></p><div class="view_article"><a href="https://doi.org/10.11648/j.fem.20220801.12" target="_blank" class="normal_link">View Article<i class="fas fa-external-link-alt"></i></a></div></td></tr></tbody></table></div></div>[18]</div>. Thus 10g of solid sludge was dissolved in 1L of sterile distilled water to form the stock solution. The pre-cultures of the 8 bacterial isolates were carried out according to the protocol of Temesgen <i>et al.</i> (2018) whose composition of the nutrient broth in grams per liter was as follows: Glucose: 3g/L; NH<sub>4</sub>CL: 3.2 g/L; KH<sub>2</sub>PO<sub>4</sub>-: 1 g/L; K<sub>2</sub>HPO<sub>4</sub>-: 10 g/L; MgSO<sub>4</sub>.7H<sub>2</sub>O: 1g/L; NaCL: 5 g/L; peptone: 5 g/L <div class="references_link" data-target="#references22"><div class="references_detail" style="display: none;" id="references22"><div class="references"><table class="normal_table"><tbody><tr><td>[19]</td><td>Temesgen, O., Diriba, M., Mulissa, J. Potential applications of some indigenous bacteria isolated from polluted areas in the treatment of brewery effluents. Biotechnology Research International. (2018), Article ID 9745198, 13 pages <p class="doi_link"><a href="https://doi.org/10.1155/2018/9745198" target="_blank" class="normal_link">https://doi.org/10.1155/2018/9745198<i class="fas fa-external-link-alt"></i></a></p><div class="view_article"><a href="https://doi.org/10.1155/2018/9745198" target="_blank" class="normal_link">View Article<i class="fas fa-external-link-alt"></i></a></div></td></tr></tbody></table></div></div>[19]</div>. Each 24-h pure isolate collected from the nutrient agar was inoculated into a vial containing 50mL of sterile nutrient broth and the set of vials was incubated on a shaker at 32°C for 48h. The liquid MSM medium supplemented with 4% stock solution of palm oil extraction sludge (v/v) used for the test has a salt composition similar to that of the pre-culture nutrient broth but free of glucose and peptone. For each series of tests, the pre-cultures of the isolates were centrifuged at 4000 rpm for 10 minutes, the resulting pellets were washed with 0.9% NaCL solution and centrifuged once more. The recovered pellets were each suspended in 20 mL of sterile 0.9% NaCL solution and calibrated with a spectrophotometer at 600 nm at OD=0.1 corresponding to a load of 107 CFU/mL. In each series of tests, 5mL of calibrated cell suspension of each isolate was inoculated in triplicate into the 100mL vials containing 45mL of sterile supplemented MSM solution. A vial containing sterile, non-inoculated supplemented liquid MSM medium into which 5 mL of 0.9% NaCL solution was introduced served as a control. OD measurements were performed after every 24 h for 7 days of incubation in a shaker at 30°C starting on day 0. OD measurements were used to determine the growth rate of each isolate after 5 days incubation according to the formula:</div><div class="Text">T (%) ₌ [(ODf-ODi)/ODi] ×100</div><div class="Text">where ODf: final optical density; ODi: initial optical density.</div><div class="Text" id="section2_2_6"><p class='Heading3'>2.2.6. Data Analyzes</p></div><div class="Text">The data collected were subjected to an analysis of variance (ANOVA) with the aim of investigating the existence of a significant difference between the isolates. The separation of isolates into homogeneous groups was carried out by the Duncan test at the α=5% threshold. The dendogram of morphological characters made it possible to group isolates with similar characters. All of these analyzes were carried out using SPSS 20.0 software.</div></div><div class="section" id="section3"><div class="Heading1">3. Results</div><div class="Text" id="section3_1"><p class='Heading2'>3.1. Evaluation of Hydrogen Potential</p></div><div class="Text">The sludge sample studied for microbiological analysis has a slightly alkaline pH of 7.3 after pH meter stability which lasted nearly 30 minutes.</div><div class="Text" id="section3_2"><p class='Heading2'>3.2. Isolation, Purification and Storage of Bacterial Isolates</p></div><div class="Text">The counts on nutrient agar obtained for the different dilutions after 48 hours of incubation provided an idea of the bacterial load of the sample. Observation of the sample shows a fairly high bacterial load around 12.6.10<sup>9</sup> CFU/g of sludge. Based on all the cultural characteristics, 31 bacteria were isolated and purified from the solid sludge sample.</div><div class="Text" id="section3_3"><p class='Heading2'>3.3. Cultural and Cellular Characterization of Bacterial Isolates</p></div><div class="Text"> <div class="Figure" id="figure_1"><img src="https://article.sciencepublishinggroup.com/article/figure/3841118/3841118_1.png"><p class="article_figures_download"><a href="https://article.sciencepublishinggroup.com/article/figure/3841118/3841118_1.png"><i class="fas fa-arrow-alt-circle-down"></i>Download: Download full-size image</a></p></div><div class="FigureCaption_SingleLine" id='figure_'1>Figure 1. <i> Cultural (A, B, C, D) and cellular (E, F, G, H) (Gx100) aspects of the respective isolates BI19, BI4, BI23 and BI12.</i></div> </div><div class="Text">All the macroscopic and microscopic characteristics studied on the 31 isolates are presented in the following two <a class='a_link' href='javascript:;' data='table_1'>Tables 1 and 2</a>. According to these results, we note the presence of significant bacterial diversity in the sludge sample. Crop characteristics vary from one isolate to another. Bacterial isolates BI1, BI2, BI3, BI4, BI8, BI14, BI15, BI16, BI18, BI19, BI21 and BI26 all have large, round, flat, rough, opaque, dry, beige-colored colonies (except Ib16 which is whitish) and develop on Mossel medium. Their cells are Gram+, rod-shaped, isolated, mobile and produce spores. Bacterial isolates BI5, BI6, BI7, BI9, BI10, BI11, BI12, BI13, BI20, BI22, BI23, BI24, BI25, BI27, BI28, BI29, BI30, BI31 all have round or irregular, beige-colored, invasive colonies, rounded, smooth and translucent. <a class='a_link' href='javascript:;' data='figure_1'>Figure 1</a> below presents the cultural and cellular aspects of some isolates. Their cells are Gram-, rod-shaped, isolated, mobile (with the exception of BI17 which is immobile). <a class='a_link' href='javascript:;' data='figure_1'>Figure 1</a> below presents the cultural and cellular aspects of some isolates. These cultural and cellular characteristics allow us to have a first idea of the type of bacteria. All 31 isolates are bacilli, including 12 Gram+ bacilli and 19 Gram- bacilli.</div><div class="Text"> <div class="Figure" id="figure_2"><img src="https://article.sciencepublishinggroup.com/article/figure/3841118/3841118_2.png"><p class="article_figures_download"><a href="https://article.sciencepublishinggroup.com/article/figure/3841118/3841118_2.png"><i class="fas fa-arrow-alt-circle-down"></i>Download: Download full-size image</a></p></div><div class="FigureCaption_SingleLine" id='figure_'2>Figure 2. <i> Hierarchical classification of bacterial isolates obtained on the basis of cultural characteristics.</i></div> </div><div class="Text">The cultural characteristics of the isolates made it possible to classify them hierarchically. The various groups of bacteria were carried out at the threshold of 95% homogeneity (<a class='a_link' href='javascript:;' data='figure_2'>Figure 2</a>). This hierarchical classification made it possible to distinguish two large groups of bacteria. Group 1 made up of 19 bacterial isolates (BI5, BI6, BI7, Ib9, BI10, BI11, BI12, BI13, BI17, BI20, BI22, BI23, BI24, BI25, BI27, BI28, BI29, BI30 and BI31) which all have the Round or irregular colonies, beige in color, invasive, rounded, smooth and translucent. Group 2 made up of 12 bacterial isolates (BI1, BI2, BI3, BI4, BI8, BI14, BI15, BI16, BI18, BI19, BI21 and Ib26) which all have large colonies, round, flat, rough, opaque, dry, of beige color (with the exception of Ib16 which is whitish) and develop on Mossel medium. Group 1 presents three subgroups: subgroup 1 consisting of bacterial isolate BI5, BI10, BI11, BI13, BI23, BI24, BI25 and BI31; subgroup 2 consisting of bacterial isolates BI6, BI7, BI9, BI12, BI20, BI22, BI27, BI28, BI29 and BI30 and subgroup 3 consisting of bacterial isolate BI17. Group 2 also presents 3 subgroups: subgroup 1 consisting of bacterial isolates BI1, BI2, BI3, BI4, BI8, BI14, BI15, BI18, BI19, BI21; subgroup 2 consisting of bacterial isolate BI26 and subgroup 3 consisting of bacterial isolate BI16.</div><div class="TableCaption_SingleLine" id="table_1">Table 1. <i> Cultural characteristics of bacterial isolates.</i></div><div class="article_table"><table frame="hsides" rules="all" cellpadding="0.0" style="width: 100%;"> <tbody> <tr> <th class="TableHead" rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 8.365470186888164%; vertical-align: middle"><p>Isolate code</p></th> <th class="TableHead" rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 8.325917136359141%; vertical-align: middle"><p>Form</p></th> <th class="TableHead" rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 8.19736972213982%; vertical-align: middle"><p>Color</p></th> <th class="TableHead" rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 9.472955601700782%; vertical-align: middle"><p>Elevation</p></th> <th class="TableHead" rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 10.629882329674677%; vertical-align: middle"><p>Outline</p></th> <th class="TableHead" rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 10.619994067042422%; vertical-align: middle"><p>Size</p></th> <th class="TableHead" rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 8.414911500049442%; vertical-align: middle"><p>Surface</p></th> <th class="TableHead" rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 11.213289824977752%; vertical-align: middle"><p>Opacity</p></th> <th class="TableHead" rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 11.213289824977752%; vertical-align: middle"><p>Consistency</p></th> <th class="TableHead" rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 13.546919806190052%; vertical-align: middle"><p>Growth on Mossel medium</p></th> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.365470186888164%; vertical-align: middle"><p>BI1</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.325917136359141%; vertical-align: middle"><p>Rounds</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.19736972213982%; vertical-align: middle"><p>Beige</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 9.472955601700782%; vertical-align: middle"><p>Flat</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.629882329674677%; vertical-align: middle"><p>Irregular</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.619994067042422%; vertical-align: middle"><p>Large</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.414911500049442%; vertical-align: middle"><p>Rough</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>Opaque</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>Dried</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 13.546919806190052%; vertical-align: middle"><p>+</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.365470186888164%; vertical-align: middle"><p>BI2</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.325917136359141%; vertical-align: middle"><p>Rounds</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.19736972213982%; vertical-align: middle"><p>Beige</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 9.472955601700782%; vertical-align: middle"><p>Flat</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.629882329674677%; vertical-align: middle"><p>Irregular</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.619994067042422%; vertical-align: middle"><p>Large</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.414911500049442%; vertical-align: middle"><p>Rough</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>Opaque</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>Dried</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 13.546919806190052%; vertical-align: middle"><p>+</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.365470186888164%; vertical-align: middle"><p>BI3</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.325917136359141%; vertical-align: middle"><p>Rounds</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.19736972213982%; vertical-align: middle"><p>Beige</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 9.472955601700782%; vertical-align: middle"><p>Flat</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.629882329674677%; vertical-align: middle"><p>Irregular</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.619994067042422%; vertical-align: middle"><p>Large</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.414911500049442%; vertical-align: middle"><p>Rough</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>Opaque</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>Dried</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 13.546919806190052%; vertical-align: middle"><p>+</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.365470186888164%; vertical-align: middle"><p>BI4</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.325917136359141%; vertical-align: middle"><p>Rounds</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.19736972213982%; vertical-align: middle"><p>Beige</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 9.472955601700782%; vertical-align: middle"><p>Flat</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.629882329674677%; vertical-align: middle"><p>Irregular</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.619994067042422%; vertical-align: middle"><p>Large</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.414911500049442%; vertical-align: middle"><p>Rough</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>Opaque</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>Dried</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 13.546919806190052%; vertical-align: middle"><p>+</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.365470186888164%; vertical-align: middle"><p>BI5</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.325917136359141%; vertical-align: middle"><p>Rounds</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.19736972213982%; vertical-align: middle"><p>Beige</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 9.472955601700782%; vertical-align: middle"><p>Domed</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.629882329674677%; vertical-align: middle"><p>NA</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.619994067042422%; vertical-align: middle"><p>Invasive</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.414911500049442%; vertical-align: middle"><p>Smooth</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>Translucent</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>NA</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 13.546919806190052%; vertical-align: middle"><p>NA</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.365470186888164%; vertical-align: middle"><p>BI6</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.325917136359141%; vertical-align: middle"><p>Irregular</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.19736972213982%; vertical-align: middle"><p>Beige</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 9.472955601700782%; vertical-align: middle"><p>Domed</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.629882329674677%; vertical-align: middle"><p>Irregular</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.619994067042422%; vertical-align: middle"><p>Punctiform</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.414911500049442%; vertical-align: middle"><p>Smooth</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>Translucent</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>NA</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 13.546919806190052%; vertical-align: middle"><p>NA</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.365470186888164%; vertical-align: middle"><p>BI7</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.325917136359141%; vertical-align: middle"><p>Irregular</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.19736972213982%; vertical-align: middle"><p>Beige</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 9.472955601700782%; vertical-align: middle"><p>Domed</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.629882329674677%; vertical-align: middle"><p>Irregular</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.619994067042422%; vertical-align: middle"><p>Punctiform</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.414911500049442%; vertical-align: middle"><p>Smooth</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>Translucent</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>NA</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 13.546919806190052%; vertical-align: middle"><p>NA</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.365470186888164%; vertical-align: middle"><p>BI8</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.325917136359141%; vertical-align: middle"><p>Rounds</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.19736972213982%; vertical-align: middle"><p>Beige</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 9.472955601700782%; vertical-align: middle"><p>Flat</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.629882329674677%; vertical-align: middle"><p>Irregular</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.619994067042422%; vertical-align: middle"><p>Large</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.414911500049442%; vertical-align: middle"><p>Rough</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>Opaque</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>Dried</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 13.546919806190052%; vertical-align: middle"><p>+</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.365470186888164%; vertical-align: middle"><p>BI9</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.325917136359141%; vertical-align: middle"><p>Irregular</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.19736972213982%; vertical-align: middle"><p>Beige</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 9.472955601700782%; vertical-align: middle"><p>Domed</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.629882329674677%; vertical-align: middle"><p>Irregular</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.619994067042422%; vertical-align: middle"><p>Punctiform</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.414911500049442%; vertical-align: middle"><p>Smooth</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>Translucent</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>NA</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 13.546919806190052%; vertical-align: middle"><p>NA</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.365470186888164%; vertical-align: middle"><p>BI10</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.325917136359141%; vertical-align: middle"><p>Rounds</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.19736972213982%; vertical-align: middle"><p>Beige</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 9.472955601700782%; vertical-align: middle"><p>Domed</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.629882329674677%; vertical-align: middle"><p>NA</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.619994067042422%; vertical-align: middle"><p>Invasive</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.414911500049442%; vertical-align: middle"><p>Smooth</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>Translucent</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>NA</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 13.546919806190052%; vertical-align: middle"><p>NA</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.365470186888164%; vertical-align: middle"><p>BI11</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.325917136359141%; vertical-align: middle"><p>Rounds</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.19736972213982%; vertical-align: middle"><p>Beige</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 9.472955601700782%; vertical-align: middle"><p>Domed</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.629882329674677%; vertical-align: middle"><p>NA</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.619994067042422%; vertical-align: middle"><p>Invasive</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.414911500049442%; vertical-align: middle"><p>Smooth</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>Translucent</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>NA</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 13.546919806190052%; vertical-align: middle"><p>NA</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.365470186888164%; vertical-align: middle"><p>BI12</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.325917136359141%; vertical-align: middle"><p>Irregular</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.19736972213982%; vertical-align: middle"><p>Beige</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 9.472955601700782%; vertical-align: middle"><p>Domed</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.629882329674677%; vertical-align: middle"><p>Irregular</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.619994067042422%; vertical-align: middle"><p>Punctiform</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.414911500049442%; vertical-align: middle"><p>Smooth</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>Translucent</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>NA</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 13.546919806190052%; vertical-align: middle"><p>NA</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.365470186888164%; vertical-align: middle"><p>BI13</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.325917136359141%; vertical-align: middle"><p>Rounds</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.19736972213982%; vertical-align: middle"><p>Beige</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 9.472955601700782%; vertical-align: middle"><p>Domed</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.629882329674677%; vertical-align: middle"><p>NA</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.619994067042422%; vertical-align: middle"><p>Invasive</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.414911500049442%; vertical-align: middle"><p>Smooth</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>Translucent</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>NA</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 13.546919806190052%; vertical-align: middle"><p>NA</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.365470186888164%; vertical-align: middle"><p>BI14</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.325917136359141%; vertical-align: middle"><p>Rounds</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.19736972213982%; vertical-align: middle"><p>Beige</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 9.472955601700782%; vertical-align: middle"><p>Flat</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.629882329674677%; vertical-align: middle"><p>Irregular</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.619994067042422%; vertical-align: middle"><p>Large</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.414911500049442%; vertical-align: middle"><p>Rough</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>Opaque</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>Dried</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 13.546919806190052%; vertical-align: middle"><p>+</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.365470186888164%; vertical-align: middle"><p>BI15</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.325917136359141%; vertical-align: middle"><p>Rounds</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.19736972213982%; vertical-align: middle"><p>Beige</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 9.472955601700782%; vertical-align: middle"><p>Flat</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.629882329674677%; vertical-align: middle"><p>Irregular</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.619994067042422%; vertical-align: middle"><p>Large</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.414911500049442%; vertical-align: middle"><p>Rough</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>Opaque</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>Dried</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 13.546919806190052%; vertical-align: middle"><p>+</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.365470186888164%; vertical-align: middle"><p>BI16</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.325917136359141%; vertical-align: middle"><p>Rounds</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.19736972213982%; vertical-align: middle"><p>Whitish</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 9.472955601700782%; vertical-align: middle"><p>Flat</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.629882329674677%; vertical-align: middle"><p>Irregular</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.619994067042422%; vertical-align: middle"><p>Large</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.414911500049442%; vertical-align: middle"><p>Rough</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>Opaque</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>Dried</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 13.546919806190052%; vertical-align: middle"><p>+</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.365470186888164%; vertical-align: middle"><p>BI17</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.325917136359141%; vertical-align: middle"><p>Rounds</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.19736972213982%; vertical-align: middle"><p>Beige</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 9.472955601700782%; vertical-align: middle"><p>Domed</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.629882329674677%; vertical-align: middle"><p>Régulières</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.619994067042422%; vertical-align: middle"><p>Small</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.414911500049442%; vertical-align: middle"><p>Smooth</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>Translucent</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>Mucosa</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 13.546919806190052%; vertical-align: middle"><p>NA</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.365470186888164%; vertical-align: middle"><p>BI18</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.325917136359141%; vertical-align: middle"><p>Rounds</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.19736972213982%; vertical-align: middle"><p>Beige</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 9.472955601700782%; vertical-align: middle"><p>Flat</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.629882329674677%; vertical-align: middle"><p>Irregular</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.619994067042422%; vertical-align: middle"><p>Large</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.414911500049442%; vertical-align: middle"><p>Rough</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>Opaque</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>Dried</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 13.546919806190052%; vertical-align: middle"><p>+</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.365470186888164%; vertical-align: middle"><p>BI19</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.325917136359141%; vertical-align: middle"><p>Rounds</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.19736972213982%; vertical-align: middle"><p>Beige</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 9.472955601700782%; vertical-align: middle"><p>Flat</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.629882329674677%; vertical-align: middle"><p>Irregular</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.619994067042422%; vertical-align: middle"><p>Large</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.414911500049442%; vertical-align: middle"><p>Rough</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>Opaque</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>Dried</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 13.546919806190052%; vertical-align: middle"><p>+</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.365470186888164%; vertical-align: middle"><p>BI20</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.325917136359141%; vertical-align: middle"><p>Irregular</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.19736972213982%; vertical-align: middle"><p>Beige</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 9.472955601700782%; vertical-align: middle"><p>Domed</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.629882329674677%; vertical-align: middle"><p>Irregular</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.619994067042422%; vertical-align: middle"><p>Punctiform</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.414911500049442%; vertical-align: middle"><p>Smooth</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>Translucent</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>NA</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 13.546919806190052%; vertical-align: middle"><p>NA</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.365470186888164%; vertical-align: middle"><p>BI21</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.325917136359141%; vertical-align: middle"><p>Rounds</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.19736972213982%; vertical-align: middle"><p>Beige</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 9.472955601700782%; vertical-align: middle"><p>Flat</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.629882329674677%; vertical-align: middle"><p>Irregular</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.619994067042422%; vertical-align: middle"><p>Large</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.414911500049442%; vertical-align: middle"><p>Rough</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>Opaque</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>Dried</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 13.546919806190052%; vertical-align: middle"><p>+</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.365470186888164%; vertical-align: middle"><p>BI22</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.325917136359141%; vertical-align: middle"><p>Irregular</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.19736972213982%; vertical-align: middle"><p>Beige</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 9.472955601700782%; vertical-align: middle"><p>Domed</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.629882329674677%; vertical-align: middle"><p>Irregular</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.619994067042422%; vertical-align: middle"><p>Punctiform</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.414911500049442%; vertical-align: middle"><p>Smooth</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>Translucent</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>NA</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 13.546919806190052%; vertical-align: middle"><p>NA</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.365470186888164%; vertical-align: middle"><p>BI23</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.325917136359141%; vertical-align: middle"><p>Rounds</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.19736972213982%; vertical-align: middle"><p>Beige</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 9.472955601700782%; vertical-align: middle"><p>Domed</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.629882329674677%; vertical-align: middle"><p>NA</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.619994067042422%; vertical-align: middle"><p>Invasives</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.414911500049442%; vertical-align: middle"><p>Smooth</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>Translucent</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>NA</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 13.546919806190052%; vertical-align: middle"><p>NA</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.365470186888164%; vertical-align: middle"><p>BI24</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.325917136359141%; vertical-align: middle"><p>Rounds</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.19736972213982%; vertical-align: middle"><p>Beige</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 9.472955601700782%; vertical-align: middle"><p>Domed</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.629882329674677%; vertical-align: middle"><p>NA</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.619994067042422%; vertical-align: middle"><p>Invasive</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.414911500049442%; vertical-align: middle"><p>Smooth</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>Translucent</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>NA</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 13.546919806190052%; vertical-align: middle"><p>NA</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.365470186888164%; vertical-align: middle"><p>BI25</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.325917136359141%; vertical-align: middle"><p>Rounds</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.19736972213982%; vertical-align: middle"><p>Beige</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 9.472955601700782%; vertical-align: middle"><p>Domed</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.629882329674677%; vertical-align: middle"><p>NA</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.619994067042422%; vertical-align: middle"><p>Invasive</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.414911500049442%; vertical-align: middle"><p>Smooth</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>Translucent</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>NA</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 13.546919806190052%; vertical-align: middle"><p>NA</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.365470186888164%; vertical-align: middle"><p>BI26</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.325917136359141%; vertical-align: middle"><p>Rounds</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.19736972213982%; vertical-align: middle"><p>Beige</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 9.472955601700782%; vertical-align: middle"><p>Domed</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.629882329674677%; vertical-align: middle"><p>Irregular</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.619994067042422%; vertical-align: middle"><p>Large</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.414911500049442%; vertical-align: middle"><p>Rough</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>Opaque</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>Dried</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 13.546919806190052%; vertical-align: middle"><p>+</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.365470186888164%; vertical-align: middle"><p>BI27</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.325917136359141%; vertical-align: middle"><p>Irregular</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.19736972213982%; vertical-align: middle"><p>Beige</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 9.472955601700782%; vertical-align: middle"><p>Domed</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.629882329674677%; vertical-align: middle"><p>Irregular</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.619994067042422%; vertical-align: middle"><p>Punctiform</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.414911500049442%; vertical-align: middle"><p>Smooth</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>Translucent</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>NA</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 13.546919806190052%; vertical-align: middle"><p>NA</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.365470186888164%; vertical-align: middle"><p>BI28</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.325917136359141%; vertical-align: middle"><p>Irregular</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.19736972213982%; vertical-align: middle"><p>Beige</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 9.472955601700782%; vertical-align: middle"><p>Domed</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.629882329674677%; vertical-align: middle"><p>Irregular</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.619994067042422%; vertical-align: middle"><p>Punctiform</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.414911500049442%; vertical-align: middle"><p>Smooth</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>Translucent</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>NA</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 13.546919806190052%; vertical-align: middle"><p>NA</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.365470186888164%; vertical-align: middle"><p>BI29</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.325917136359141%; vertical-align: middle"><p>Irregular</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.19736972213982%; vertical-align: middle"><p>Beige</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 9.472955601700782%; vertical-align: middle"><p>Domed</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.629882329674677%; vertical-align: middle"><p>Irregular</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.619994067042422%; vertical-align: middle"><p>Punctiform</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.414911500049442%; vertical-align: middle"><p>Smooth</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>Translucent</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>NA</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 13.546919806190052%; vertical-align: middle"><p>NA</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.365470186888164%; vertical-align: middle"><p>BI30</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.325917136359141%; vertical-align: middle"><p>Irregular</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.19736972213982%; vertical-align: middle"><p>Beige</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 9.472955601700782%; vertical-align: middle"><p>Domed</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.629882329674677%; vertical-align: middle"><p>Irregular</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 10.619994067042422%; vertical-align: middle"><p>Punctiform</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 8.414911500049442%; vertical-align: middle"><p>Smooth</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>Translucent</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>NA</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 13.546919806190052%; vertical-align: middle"><p>NA</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 8.365470186888164%; vertical-align: middle"><p>BI31</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 8.325917136359141%; vertical-align: middle"><p>Rounds</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 8.19736972213982%; vertical-align: middle"><p>Beige</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 9.472955601700782%; vertical-align: middle"><p>Domed</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 10.629882329674677%; vertical-align: middle"><p>NA</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 10.619994067042422%; vertical-align: middle"><p>Invasive</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 8.414911500049442%; vertical-align: middle"><p>Smooth</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>Translucent</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 11.213289824977752%; vertical-align: middle"><p>NA</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 13.546919806190052%; vertical-align: middle"><p>NA</p></td> </tr> </tbody> </table></div><div class="Text">+: growth on Mossel medium plus polymixin B; NA: not applied.</div><div class="TableCaption_SingleLine" id="table_2">Table 2. <i> Cellular characteristics of bacterial isolates.</i></div><div class="article_table"><table frame="hsides" rules="all" cellpadding="0.0" style="width: 100%;"> <tbody> <tr> <th class="TableHead" rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 16.66503475962009%; vertical-align: middle"><p>Isolate code</p></th> <th class="TableHead" rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 16.66503475962009%; vertical-align: middle"><p>Gram stain</p></th> <th class="TableHead" rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 16.66503475962009%; vertical-align: middle"><p>cell shape</p></th> <th class="TableHead" rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 18.055419563301676%; vertical-align: middle"><p>arrangement</p></th> <th class="TableHead" rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 16.655243317340645%; vertical-align: middle"><p>Mobility</p></th> <th class="TableHead" rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 15.294232840497404%; vertical-align: middle"><p>sporulation</p></th> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>BI1</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>Gram +</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>stick</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 18.055419563301676%; vertical-align: middle"><p>Isolated</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.655243317340645%; vertical-align: middle"><p>Mobile</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 15.294232840497404%; vertical-align: middle"><p>+</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>BI2</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>Gram +</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>stick</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 18.055419563301676%; vertical-align: middle"><p>Isolated</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.655243317340645%; vertical-align: middle"><p>Mobile</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 15.294232840497404%; vertical-align: middle"><p>+</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>BI3</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>Gram +</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>stick</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 18.055419563301676%; vertical-align: middle"><p>Isolated</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.655243317340645%; vertical-align: middle"><p>Mobile</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 15.294232840497404%; vertical-align: middle"><p>+</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>BI4</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>Gram +</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>stick</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 18.055419563301676%; vertical-align: middle"><p>Isolated</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.655243317340645%; vertical-align: middle"><p>Mobile</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 15.294232840497404%; vertical-align: middle"><p>+</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>BI5</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>Gram -</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>stick</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 18.055419563301676%; vertical-align: middle"><p>Isolated</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.655243317340645%; vertical-align: middle"><p>Mobile</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 15.294232840497404%; vertical-align: middle"><p>NA</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>BI6</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>Gram -</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>stick</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 18.055419563301676%; vertical-align: middle"><p>Isolated</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.655243317340645%; vertical-align: middle"><p>Mobile</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 15.294232840497404%; vertical-align: middle"><p>NA</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>BI7</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>Gram -</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>stick</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 18.055419563301676%; vertical-align: middle"><p>Isolated</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.655243317340645%; vertical-align: middle"><p>Mobile</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 15.294232840497404%; vertical-align: middle"><p>NA</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>BI8</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>Gram +</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>stick</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 18.055419563301676%; vertical-align: middle"><p>Isolated</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.655243317340645%; vertical-align: middle"><p>Mobile</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 15.294232840497404%; vertical-align: middle"><p>+</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>BI9</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>Gram -</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>stick</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 18.055419563301676%; vertical-align: middle"><p>Isolated</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.655243317340645%; vertical-align: middle"><p>Mobile</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 15.294232840497404%; vertical-align: middle"><p>NA</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>BI10</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>Gram-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>stick</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 18.055419563301676%; vertical-align: middle"><p>Isolated</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.655243317340645%; vertical-align: middle"><p>Mobile</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 15.294232840497404%; vertical-align: middle"><p>NA</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>BI11</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>Gram-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>stick</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 18.055419563301676%; vertical-align: middle"><p>Isolated</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.655243317340645%; vertical-align: middle"><p>Mobile</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 15.294232840497404%; vertical-align: middle"><p>NA</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>BI12</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>Gram-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>stick</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 18.055419563301676%; vertical-align: middle"><p>Isolated</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.655243317340645%; vertical-align: middle"><p>Mobile</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 15.294232840497404%; vertical-align: middle"><p>NA</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>BI13</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>Gram-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>stick</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 18.055419563301676%; vertical-align: middle"><p>Isolated</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.655243317340645%; vertical-align: middle"><p>Mobile</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 15.294232840497404%; vertical-align: middle"><p>NA</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>BI14</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>Gram +</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>stick</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 18.055419563301676%; vertical-align: middle"><p>Isolated</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.655243317340645%; vertical-align: middle"><p>Mobile</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 15.294232840497404%; vertical-align: middle"><p>+</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>BI15</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>Gram +</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>stick</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 18.055419563301676%; vertical-align: middle"><p>Isolated</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.655243317340645%; vertical-align: middle"><p>Mobile</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 15.294232840497404%; vertical-align: middle"><p>+</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>BI16</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>Gram +</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>stick</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 18.055419563301676%; vertical-align: middle"><p>Isolated</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.655243317340645%; vertical-align: middle"><p>Mobile</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 15.294232840497404%; vertical-align: middle"><p>+</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>BI17</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>Gram-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>stick</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 18.055419563301676%; vertical-align: middle"><p>Isolated</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.655243317340645%; vertical-align: middle"><p>Immobile</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 15.294232840497404%; vertical-align: middle"><p>NA</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>BI18</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>Gram +</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>stick</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 18.055419563301676%; vertical-align: middle"><p>Isolated</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.655243317340645%; vertical-align: middle"><p>Mobile</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 15.294232840497404%; vertical-align: middle"><p>+</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>BI19</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>Gram +</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>stick</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 18.055419563301676%; vertical-align: middle"><p>Isolated</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.655243317340645%; vertical-align: middle"><p>Mobile</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 15.294232840497404%; vertical-align: middle"><p>+</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>BI20</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>Gram-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>stick</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 18.055419563301676%; vertical-align: middle"><p>Isolated</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.655243317340645%; vertical-align: middle"><p>Mobile</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 15.294232840497404%; vertical-align: middle"><p>NA</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>BI21</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>Gram +</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>stick</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 18.055419563301676%; vertical-align: middle"><p>Isolated</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.655243317340645%; vertical-align: middle"><p>Mobile</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 15.294232840497404%; vertical-align: middle"><p>+</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>BI22</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>Gram -</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>stick</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 18.055419563301676%; vertical-align: middle"><p>Isolated</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.655243317340645%; vertical-align: middle"><p>Mobile</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 15.294232840497404%; vertical-align: middle"><p>NA</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>BI23</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>Gram -</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>stick</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 18.055419563301676%; vertical-align: middle"><p>Isolated</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.655243317340645%; vertical-align: middle"><p>Mobile</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 15.294232840497404%; vertical-align: middle"><p>NA</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>BI24</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>Gram -</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>stick</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 18.055419563301676%; vertical-align: middle"><p>Isolated</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.655243317340645%; vertical-align: middle"><p>Mobile</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 15.294232840497404%; vertical-align: middle"><p>NA</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>BI25</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>Gram-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>stick</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 18.055419563301676%; vertical-align: middle"><p>Isolated</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.655243317340645%; vertical-align: middle"><p>Mobile</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 15.294232840497404%; vertical-align: middle"><p>NA</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>BI26</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>Gram+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>stick</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 18.055419563301676%; vertical-align: middle"><p>Isolated</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.655243317340645%; vertical-align: middle"><p>mobile</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 15.294232840497404%; vertical-align: middle"><p>+</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>BI27</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>Gram-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>stick</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 18.055419563301676%; vertical-align: middle"><p>Isolated</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.655243317340645%; vertical-align: middle"><p>Mobile</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 15.294232840497404%; vertical-align: middle"><p>NA</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>BI28</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>Gram -</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>stick</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 18.055419563301676%; vertical-align: middle"><p>Isolated</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.655243317340645%; vertical-align: middle"><p>Mobile</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 15.294232840497404%; vertical-align: middle"><p>NA</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>BI29</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>Gram -</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>stick</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 18.055419563301676%; vertical-align: middle"><p>Isolated</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.655243317340645%; vertical-align: middle"><p>Mobile</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 15.294232840497404%; vertical-align: middle"><p>NA</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>BI30</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>Gram-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>stick</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 18.055419563301676%; vertical-align: middle"><p>Isolated</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 16.655243317340645%; vertical-align: middle"><p>Mobile</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 15.294232840497404%; vertical-align: middle"><p>NA</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>BI31</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>Gram-</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 16.66503475962009%; vertical-align: middle"><p>stick</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 18.055419563301676%; vertical-align: middle"><p>Isolated</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 16.655243317340645%; vertical-align: middle"><p>Mobile</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 15.294232840497404%; vertical-align: middle"><p>NA</p></td> </tr> </tbody> </table></div><div class="Text">NA: not applied; +: positive sporulation.</div><div class="Text" id="section3_4"><p class='Heading2'>3.4. Biochemical Characterization of Isolates</p></div><div class="Text" id="section3_4_1"><p class='Heading3'>3.4.1. Characterization of Gram+ Bacteria</p></div><div class="Text">The search for respiratory enzymes such as oxidase and catalase made it possible to characterize the Gram+ bacteria isolated from the sludge sample. The reactions of the bacterial colonies deposited on an oxidase disk soaked in a drop of distilled water led to the immediate appearance of a purple color (positive test) in all the gram+ bacteria, indicating the presence of phenylenediamine- oxidase or cytochrome oxidase in these bacteria.</div><div class="Text">The reactions of bacterial colonies emulsified in a drop of hydrogen peroxide on a glass slide led to the immediate appearance of gas bubbles (effervescence) corresponding to the oxygen released in all Gram+ bacteria, thus indicating the presence of catalase in the bacteria tested.</div><div class="Text">On the basis of the cultural (shape, color and size of colonies), cellular (color, shape, Gram staining, shape, arrangement, mobility, sporulation and growth on Mossel medium) and biochemical (catalase and oxidase) characteristics obtained, the isolates can be identified to the genus level. The bacterial isolates BI1, BI2, BI3, BI4, BI8, BI14, BI15, BI16, BI18, BI19, BI21 and BI26 all have large colonies, circular in shape, beige in color (with the exception of BI16 which is whitish) and develop in the Mossel environment. Their cells are Gram+, rod-shaped, isolated, mobile and produce spores. Biochemical tests indicate that all of these bacteria produce catalase (<a class='a_link' href='javascript:;' data='table_3'>Table 3</a>). All of these characteristics lead to their identification as belonging to the genus <i>Bacillus sp</i>.</div><div class="TableCaption_SingleLine" id="table_3">Table 3. <i> Results of oxidase and catalase tests in gram+ bacteria.</i></div><div class="article_table"><table frame="hsides" rules="all" cellpadding="0.0" style="width: 100%;"> <tbody> <tr> <th class="TableHead" rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 25.0%; vertical-align: middle"><p>Stem code</p></th> <th class="TableHead" rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 25.0%; vertical-align: middle"><p>Gram test</p></th> <th class="TableHead" rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 25.0%; vertical-align: middle"><p>Catalase test</p></th> <th class="TableHead" rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 25.0%; vertical-align: middle"><p>Oxidase test</p></th> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>BI1</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Gram +</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>BI2</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Gram +</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>BI3</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Gram +</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>BI4</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Gram +</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>BI5</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Gram -</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Negatif</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>BI6</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Gram -</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>BI7</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Gram -</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>BI8</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Gram +</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>BI9</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Gram -</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>BI10</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Gram-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Negatif</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>BI11</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Gram-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Negatif</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>BI12</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Gram-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>BI13</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Gram-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Negatif</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>BI14</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Gram +</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>BI15</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Gram +</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>BI16</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Gram +</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>BI17</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Gram-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Negatif</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>BI18</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Gram +</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>BI19</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Gram +</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>BI20</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Gram-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>BI21</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Gram +</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>BI22</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Gram -</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>BI23</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Gram -</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Negatif</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>BI24</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Gram -</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Negatif</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>BI25</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Gram-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Negatif</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>BI26</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Gram +</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>BI27</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Gram-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>BI28</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Gram -</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>BI29</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Gram -</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>BI30</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Gram-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>BI31</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Gram-</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Positive</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 25.0%; vertical-align: middle"><p>Negatif</p></td> </tr> </tbody> </table></div><div class="Text" id="section3_4_2"><p class='Heading3'>3.4.2. Characterization of Gram- Bacteria</p></div><div class="Text">The results of the biochemical tests carried out on Gram- bacteria are grouped in <a class='a_link' href='javascript:;' data='table_4'>Tables 4 and 5</a>. On the basis of the cultural, cellular and biochemical characteristics obtained, certain strains can be identified at the genus or species level. Bacterial isolates BI5, BI6, BI7, BI9, BI10, BI11, BI12, BI13, BI20, BI22, BI23, BI24, BI25, BI27, BI28, BI29, BI30, BI31 all have round or irregular, beige-colored, invasive colonies, smooth and translucent. Their cells are Gram-, rod-shaped, isolated, mobile (with the exception of Ib17 which is immobile). Biochemical tests indicated that all of these bacteria produce catalase. In addition, the ONPG, CIT, GEL and OX tests are positive for bacterial isolates BI6, BI7, BI9, BI12, BI20, BI22, BI27, BI28, BI29 and BI30. These isolates are identified as belonging to the genus <i>Pseudomonas sp</i>. However, ODC (Ornithine decarboxylase), CIT (Citrate), IND (Indole), VP (Voges-Proskauer), GEL (Gelatin), GLU (Glucose), MAN (Mannose), INO (Inositol), SOR (Sorbitol), RHA (Rhamnose), BAG (Sucrose), MEL (Melibiosis), AMY (Amygdalin) and ARA (Arabinose) tests are positive for bacterial isolates BI5, BI10, BI11, BI13, BI23, BI24, BI25 and BI31. These isolates are identified as belonging to the species <i>Proteus mirabilis</i>. However, ONPG (O-nitrophényl- D-galactopyranoside), ADH (Arginine dihydrolase), LDC (Lysine decarboxylase), URE (Urea), CIT (Citrate), IND (Indole), VP (Voges-Proskauer), GEL (Gelatin), GLU (Glucose), MAN (Mannose), INO (Inositol), SOR (Sorbitol), RHA (Rhamnose), BAG (Sucrose), MEL (Melibiosis), AMY (Amygdalin) and ARA (Arabinose) are positive for bacterial isolate BI17. This bacterial isolate is identified as belonging to the species <i>Klebsiella pneumoniae</i>.</div><div class="TableCaption_SingleLine" id="table_4">Table 4. <i> Results of the 11 biochemical tests of Gram- bacteria based on the API20E Kit and the oxidase test.</i></div><div class="article_table"><table frame="hsides" rules="all" cellpadding="0.0" style="width: 100%;"> <tbody> <tr> <th class="TableHead" rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 6.619014980906687%; vertical-align: middle"><p></p></th> <th class="TableHead" rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 7.30441594046803%; vertical-align: middle"><p>ONPG</p></th> <th class="TableHead" rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 6.658180750024479%; vertical-align: middle"><p>ADH</p></th> <th class="TableHead" rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 6.658180750024479%; vertical-align: middle"><p>LDC</p></th> <th class="TableHead" rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 6.6679721923039255%; vertical-align: middle"><p>ODC</p></th> <th class="TableHead" rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 6.658180750024479%; vertical-align: middle"><p>CIT</p></th> <th class="TableHead" rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 6.6679721923039255%; vertical-align: middle"><p>H<sub>2</sub>S</p></th> <th class="TableHead" rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 6.658180750024479%; vertical-align: middle"><p>URE</p></th> <th class="TableHead" rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 6.658180750024479%; vertical-align: middle"><p>TDA</p></th> <th class="TableHead" rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 6.6679721923039255%; vertical-align: middle"><p>IND</p></th> <th class="TableHead" rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 6.658180750024479%; vertical-align: middle"><p>VP</p></th> <th class="TableHead" rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 6.6679721923039255%; vertical-align: middle"><p>GEL</p></th> <th class="TableHead" rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 19.455595809262704%; vertical-align: middle"><p>Species name</p></th> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.619014980906687%; vertical-align: middle"><p>BI23</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.30441594046803%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 19.455595809262704%; vertical-align: middle"><p><i>Proteus mirabilis</i></p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.619014980906687%; vertical-align: middle"><p>BI29</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.30441594046803%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 19.455595809262704%; vertical-align: middle"><p><i>Pseudomonas </i><i>sp</i></p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.619014980906687%; vertical-align: middle"><p>BI6</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.30441594046803%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 19.455595809262704%; vertical-align: middle"><p><i>Pseudomonas </i><i>sp</i></p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.619014980906687%; vertical-align: middle"><p>BI24</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.30441594046803%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 19.455595809262704%; vertical-align: middle"><p><i>Proteus mirabilis</i></p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.619014980906687%; vertical-align: middle"><p>BI5</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.30441594046803%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 19.455595809262704%; vertical-align: middle"><p><i>Proteus mirabilis</i></p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.619014980906687%; vertical-align: middle"><p>BI7</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.30441594046803%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 19.455595809262704%; vertical-align: middle"><p><i>Pseudomonas </i><i>sp</i></p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.619014980906687%; vertical-align: middle"><p>BI28</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.30441594046803%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 19.455595809262704%; vertical-align: middle"><p><i>Pseudomonas </i><i>sp</i></p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.619014980906687%; vertical-align: middle"><p>BI22</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.30441594046803%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 19.455595809262704%; vertical-align: middle"><p><i>Pseudomonas </i><i>sp</i></p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.619014980906687%; vertical-align: middle"><p>BI20</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.30441594046803%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 19.455595809262704%; vertical-align: middle"><p><i>Pseudomonas </i><i>sp</i></p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.619014980906687%; vertical-align: middle"><p>BI30</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.30441594046803%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 19.455595809262704%; vertical-align: middle"><p><i>Pseudomonas </i><i>sp</i></p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.619014980906687%; vertical-align: middle"><p>BI10</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.30441594046803%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 19.455595809262704%; vertical-align: middle"><p><i>Proteus mirabilis</i></p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.619014980906687%; vertical-align: middle"><p>BI25</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.30441594046803%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 19.455595809262704%; vertical-align: middle"><p><i>Proteus mirabilis</i></p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.619014980906687%; vertical-align: middle"><p>BI12</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.30441594046803%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 19.455595809262704%; vertical-align: middle"><p><i>Pseudomonas </i><i>sp</i></p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.619014980906687%; vertical-align: middle"><p>BI27</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.30441594046803%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 19.455595809262704%; vertical-align: middle"><p><i>Pseudomonas </i><i>sp</i></p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.619014980906687%; vertical-align: middle"><p>BI13</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.30441594046803%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 19.455595809262704%; vertical-align: middle"><p><i>Proteus mirabilis</i></p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.619014980906687%; vertical-align: middle"><p>BI31</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.30441594046803%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 19.455595809262704%; vertical-align: middle"><p><i>Proteus mirabilis</i></p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.619014980906687%; vertical-align: middle"><p>BI17</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.30441594046803%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 19.455595809262704%; vertical-align: middle"><p><i>Klebsiella</i><i> </i><i>pneumoniae</i></p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.619014980906687%; vertical-align: middle"><p>BI11</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.30441594046803%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 19.455595809262704%; vertical-align: middle"><p><i>Proteus mirabilis</i></p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 6.619014980906687%; vertical-align: middle"><p>BI9</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 7.30441594046803%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 6.658180750024479%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 6.6679721923039255%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 19.455595809262704%; vertical-align: middle"><p><i>Pseudomonas </i><i>sp</i></p></td> </tr> </tbody> </table></div><div class="Text">+: positive test; -: negative test.</div><div class="TableCaption_SingleLine" id="table_5">Table 5. <i> Results of the 10 biochemical tests of Gram- bacteria based on the API20E Kit and the oxidase test.</i></div><div class="article_table"><table frame="hsides" rules="all" cellpadding="0.0" style="width: 100%;"> <tbody> <tr> <th class="TableHead" rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 7.3142073827474805%; vertical-align: middle"><p></p></th> <th class="TableHead" rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 7.323998825026928%; vertical-align: middle"><p>GLU</p></th> <th class="TableHead" rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 7.323998825026928%; vertical-align: middle"><p>MAN</p></th> <th class="TableHead" rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 7.323998825026928%; vertical-align: middle"><p>INO</p></th> <th class="TableHead" rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 7.323998825026928%; vertical-align: middle"><p>SOR</p></th> <th class="TableHead" rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 7.3142073827474805%; vertical-align: middle"><p>RHA</p></th> <th class="TableHead" rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 7.323998825026928%; vertical-align: middle"><p>BAG</p></th> <th class="TableHead" rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 7.323998825026928%; vertical-align: middle"><p>MEL</p></th> <th class="TableHead" rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 7.323998825026928%; vertical-align: middle"><p>AMY</p></th> <th class="TableHead" rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 7.323998825026928%; vertical-align: middle"><p>ARA</p></th> <th class="TableHead" rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 7.323998825026928%; vertical-align: middle"><p>OX</p></th> <th class="TableHead" rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 19.45559580926271%; vertical-align: middle"><p>Species name</p></th> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.3142073827474805%; vertical-align: middle"><p>BI23</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.3142073827474805%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 19.45559580926271%; vertical-align: middle"><p><i>Proteus mirabilis</i></p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.3142073827474805%; vertical-align: middle"><p>BI29</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.3142073827474805%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 19.45559580926271%; vertical-align: middle"><p><i>Pseudomonas </i><i>sp</i></p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.3142073827474805%; vertical-align: middle"><p>BI6</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.3142073827474805%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 19.45559580926271%; vertical-align: middle"><p><i>Pseudomonas </i><i>sp</i></p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.3142073827474805%; vertical-align: middle"><p>BI24</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.3142073827474805%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 19.45559580926271%; vertical-align: middle"><p><i>Proteus mirabilis</i></p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.3142073827474805%; vertical-align: middle"><p>BI5</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.3142073827474805%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 19.45559580926271%; vertical-align: middle"><p><i>Proteus mirabilis</i></p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.3142073827474805%; vertical-align: middle"><p>BI7</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.3142073827474805%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 19.45559580926271%; vertical-align: middle"><p><i>Pseudomonas </i><i>sp</i></p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.3142073827474805%; vertical-align: middle"><p>BI28</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.3142073827474805%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 19.45559580926271%; vertical-align: middle"><p><i>Pseudomonas </i><i>sp</i></p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.3142073827474805%; vertical-align: middle"><p>BI22</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.3142073827474805%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 19.45559580926271%; vertical-align: middle"><p><i>Pseudomonas </i><i>sp</i></p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.3142073827474805%; vertical-align: middle"><p>BI20</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.3142073827474805%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 19.45559580926271%; vertical-align: middle"><p><i>Pseudomonas </i><i>sp</i></p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.3142073827474805%; vertical-align: middle"><p>BI30</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.3142073827474805%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 19.45559580926271%; vertical-align: middle"><p><i>Pseudomonas </i><i>sp</i></p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.3142073827474805%; vertical-align: middle"><p>BI10</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.3142073827474805%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 19.45559580926271%; vertical-align: middle"><p><i>Proteus mirabilis</i></p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.3142073827474805%; vertical-align: middle"><p>BI25</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.3142073827474805%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 19.45559580926271%; vertical-align: middle"><p><i>Proteus mirabilis</i></p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.3142073827474805%; vertical-align: middle"><p>BI12</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.3142073827474805%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 19.45559580926271%; vertical-align: middle"><p><i>Pseudomonas </i><i>sp</i></p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.3142073827474805%; vertical-align: middle"><p>BI27</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.3142073827474805%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 19.45559580926271%; vertical-align: middle"><p><i>Pseudomonas </i><i>sp</i></p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.3142073827474805%; vertical-align: middle"><p>BI13</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.3142073827474805%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 19.45559580926271%; vertical-align: middle"><p><i>Proteus mirabilis</i></p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.3142073827474805%; vertical-align: middle"><p>BI31</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.3142073827474805%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 19.45559580926271%; vertical-align: middle"><p><i>Proteus mirabilis</i></p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.3142073827474805%; vertical-align: middle"><p>BI17</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.3142073827474805%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 19.45559580926271%; vertical-align: middle"><p><i>Klebsiella</i><i> </i><i>pneumoniae</i></p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.3142073827474805%; vertical-align: middle"><p>BI11</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.3142073827474805%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 19.45559580926271%; vertical-align: middle"><p><i>Proteus mirabilis</i></p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 7.3142073827474805%; vertical-align: middle"><p>BI9</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 7.3142073827474805%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>-</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 7.323998825026928%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 19.45559580926271%; vertical-align: middle"><p><i>Pseudomonas </i><i>sp</i></p></td> </tr> </tbody> </table></div><div class="Text">+: positive test; -: negative test.</div><div class="Text" id="section3_5"><p class='Heading2'>3.5. Biodegradability Test of Palm Oil and Extraction Sludge Using Bacterial Isolates</p></div><div class="Text" id="section3_5_1"><p class='Heading3'>3.5.1. Palm Oil Biodegradability Test</p></div><div class="Text">The assessment of palm oil biodegradability is based on the ability of isolates to use palm oil as the sole source of carbon and energy. <a class='a_link' href='javascript:;' data='figure_3'>Figure 3</a> shows some images of the growth of the isolates on M2 medium supplemented with 2% palm oil as the sole carbon source and on M2 medium supplemented with 2% palm oil plus yeast extract. It appears that all the bacterial isolates tested grew on the M2 medium supplemented with 2% palm oil and devoid of yeast extract after 48 hours with a difference in the load. Unlike, in boxes containing M2 medium with 2% palm oil and 1g/L of yeast extract, growth is obtained after 24 hours with a heavier load. <a class='a_link' href='javascript:;' data='table_6'>Table 6</a> presents the results of the biodegradability test of palm oil by the different bacterial isolates. We observe that all the isolates grew in the presence of palm oil as the sole source of carbon and energy but with a difference in the load. Bacterial isolates BI2, BI5, BI9, BI10, BI13, BI22, BI29 and BI31 ²showed growth with higher load. These isolates were selected for further work.</div><div class="Text"> <div class="Figure" id="figure_3"><img src="https://article.sciencepublishinggroup.com/article/figure/3841118/3841118_3.png"><p class="article_figures_download"><a href="https://article.sciencepublishinggroup.com/article/figure/3841118/3841118_3.png"><i class="fas fa-arrow-alt-circle-down"></i>Download: Download full-size image</a></p></div><div class="FigureCaption_SingleLine" id='figure_'3>Figure 3. <i> Some images of the growth of bacterial isolates on M2 medium with 2% palm oil as the sole carbon source (A, B, C) and on M2 medium supplemented with palm oil plus yeast extract (D, E, F).</i></div> </div><div class="TableCaption_SingleLine" id="table_6">Table 6. <i> Test de biodégradabilité de l’huile de palme par les différents isolats bactériens.</i></div><div class="article_table"><table frame="hsides" rules="all" cellpadding="0.0" style="width: 100%;"> <tbody> <tr> <th class="TableHead" rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 23.25467541368844%; vertical-align: middle"><p>Bacterial isolates</p></th> <th class="TableHead" rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 33.15382355821013%; vertical-align: middle"><p>Growth in the presence of palm oil</p></th> <th class="TableHead" rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: bold; background-color: #DAEEF3; width: 43.59150102810144%; vertical-align: middle"><p>Growth in the presence of palm oil and yeast extract</p></th> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 23.25467541368844%; vertical-align: middle"><p>BI1</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 33.15382355821013%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 43.59150102810144%; vertical-align: middle"><p>++</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 23.25467541368844%; vertical-align: middle"><p>BI2</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 33.15382355821013%; vertical-align: middle"><p>++</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 43.59150102810144%; vertical-align: middle"><p>++++</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 23.25467541368844%; vertical-align: middle"><p>BI3</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 33.15382355821013%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 43.59150102810144%; vertical-align: middle"><p>++</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 23.25467541368844%; vertical-align: middle"><p>BI4</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 33.15382355821013%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 43.59150102810144%; vertical-align: middle"><p>++</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 23.25467541368844%; vertical-align: middle"><p>BI 5</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 33.15382355821013%; vertical-align: middle"><p>++</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 43.59150102810144%; vertical-align: middle"><p>++++</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 23.25467541368844%; vertical-align: middle"><p>BI6</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 33.15382355821013%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 43.59150102810144%; vertical-align: middle"><p>++</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 23.25467541368844%; vertical-align: middle"><p>BI7</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 33.15382355821013%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 43.59150102810144%; vertical-align: middle"><p>++</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 23.25467541368844%; vertical-align: middle"><p>BI8</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 33.15382355821013%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 43.59150102810144%; vertical-align: middle"><p>++</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 23.25467541368844%; vertical-align: middle"><p>BI9</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 33.15382355821013%; vertical-align: middle"><p>++</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 43.59150102810144%; vertical-align: middle"><p>++++</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 23.25467541368844%; vertical-align: middle"><p>BI10</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 33.15382355821013%; vertical-align: middle"><p>++</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 43.59150102810144%; vertical-align: middle"><p>++++</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 23.25467541368844%; vertical-align: middle"><p>BI11</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 33.15382355821013%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 43.59150102810144%; vertical-align: middle"><p>++</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 23.25467541368844%; vertical-align: middle"><p>BI12</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 33.15382355821013%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 43.59150102810144%; vertical-align: middle"><p>++</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 23.25467541368844%; vertical-align: middle"><p>BI13</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 33.15382355821013%; vertical-align: middle"><p>++</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 43.59150102810144%; vertical-align: middle"><p>++++</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 23.25467541368844%; vertical-align: middle"><p>BI14</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 33.15382355821013%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 43.59150102810144%; vertical-align: middle"><p>++</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 23.25467541368844%; vertical-align: middle"><p>BI15</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 33.15382355821013%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 43.59150102810144%; vertical-align: middle"><p>++</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 23.25467541368844%; vertical-align: middle"><p>BI16</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 33.15382355821013%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 43.59150102810144%; vertical-align: middle"><p>++</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 23.25467541368844%; vertical-align: middle"><p>BI17</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 33.15382355821013%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 43.59150102810144%; vertical-align: middle"><p>++</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 23.25467541368844%; vertical-align: middle"><p>BI18</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 33.15382355821013%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 43.59150102810144%; vertical-align: middle"><p>++</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 23.25467541368844%; vertical-align: middle"><p>BI19</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 33.15382355821013%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 43.59150102810144%; vertical-align: middle"><p>++</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 23.25467541368844%; vertical-align: middle"><p>BI20</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 33.15382355821013%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 43.59150102810144%; vertical-align: middle"><p>++</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 23.25467541368844%; vertical-align: middle"><p>BI21</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 33.15382355821013%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 43.59150102810144%; vertical-align: middle"><p>++</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 23.25467541368844%; vertical-align: middle"><p>BI22</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 33.15382355821013%; vertical-align: middle"><p>++</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 43.59150102810144%; vertical-align: middle"><p>++++</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 23.25467541368844%; vertical-align: middle"><p>BI23</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 33.15382355821013%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 43.59150102810144%; vertical-align: middle"><p>++</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 23.25467541368844%; vertical-align: middle"><p>BI24</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 33.15382355821013%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 43.59150102810144%; vertical-align: middle"><p>++</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 23.25467541368844%; vertical-align: middle"><p>BI25</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 33.15382355821013%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 43.59150102810144%; vertical-align: middle"><p>++</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 23.25467541368844%; vertical-align: middle"><p>BI26</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 33.15382355821013%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 43.59150102810144%; vertical-align: middle"><p>++</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 23.25467541368844%; vertical-align: middle"><p>BI27</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 33.15382355821013%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 43.59150102810144%; vertical-align: middle"><p>++</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 23.25467541368844%; vertical-align: middle"><p>BI28</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 33.15382355821013%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 43.59150102810144%; vertical-align: middle"><p>++</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 23.25467541368844%; vertical-align: middle"><p>BI29</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 33.15382355821013%; vertical-align: middle"><p>++</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 43.59150102810144%; vertical-align: middle"><p>++++</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 23.25467541368844%; vertical-align: middle"><p>BI30</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 33.15382355821013%; vertical-align: middle"><p>+</p></td> <td rowspan="1" colspan="1" valign="middle" style="border-style: solid none solid; border-width: 0.0 0 0.0; font-weight: normal; background-color: white; width: 43.59150102810144%; vertical-align: middle"><p>++</p></td> </tr> <tr> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 23.25467541368844%; vertical-align: middle"><p>BI31</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 33.15382355821013%; vertical-align: middle"><p>++</p></td> <td rowspan="1" colspan="1" valign="middle" align="left" style="border-style: solid none solid; border-width: 0.0 0 1.0; font-weight: normal; background-color: white; width: 43.59150102810144%; vertical-align: middle"><p>++++</p></td> </tr> </tbody> </table></div><div class="Text">+ = growth; ++ = growth with high load; ++++ = growth with higher load.</div><div class="Text" id="section3_5_2"><p class='Heading3'>3.5.2. Biodegradability Test of Solid Sludge from Palm Oil Extraction</p></div><div class="Text">The growth kinetics of the isolates in the presence of solid sludge from palm oil extraction as the sole source of carbon and energy was measured after 7 days (<a class='a_link' href='javascript:;' data='figure_4'>Figure 4</a>). Through the results obtained, we see that the bacterial strains studied show 5 growth phases. An acceleration phase between D0 and D1, a slowdown phase between D1 and D3, an exponential growth phase between D3 and D5, a decline phase between D5 and D6, then a second acceleration phase between D6 and D7. The growth curves in <a class='a_link' href='javascript:;' data='figure_4'>Figure 4</a> show that the growth speed of the isolates and consequently that of the degradation of palm oil extraction sludge is very significant during the first 5 days. This growth is continuous during the first 5 days of incubation with a maximum number of microorganisms on the 5th day of incubation in all isolates. The results of optical density (OD) show that all bacterial isolates tested have the ability to use palm oil sludge as the sole source of carbon and energy, but to different degrees. Bacterial isolates BI5, BI31 and BI29 exhibit the highest growth rates, respectively. Bacterial isolate BI9 presents the lowest growth rate. We also note that most of the strains show good growth over the entire duration of the incubation and this growth appears stationary. Nevertheless, we observed a drop in growth between day 5 and day 6, then a recovery between day 6 and day 7 in all isolates.</div><div class="Text">The results of the evolution of the biomass of the cell suspension of each isolate inoculated in the liquid MSM medium supplemented with 4% stock solution of palm oil extraction sludge after 5 days of incubation are presented in the <a class='a_link' href='javascript:;' data='figure_5'>Figure 5</a> below. Analysis of variance of the growth rate of the different isolates showed a significant difference (p = 0.013). These results showed a variation in the biomass of the isolates between 16 and 187.2%. Bacterial isolates BI2, BI5, BI31 and BI10 showed the highest growth rates. Bacterial isolates BI29 and BI22 showed the lowest growth rates. 75% of isolates had a growth rate greater than 50%.</div><div class="Text"> <div class="Figure" id="figure_4"><img src="https://article.sciencepublishinggroup.com/article/figure/3841118/3841118_4.png"><p class="article_figures_download"><a href="https://article.sciencepublishinggroup.com/article/figure/3841118/3841118_4.png"><i class="fas fa-arrow-alt-circle-down"></i>Download: Download full-size image</a></p></div><div class="FigureCaption_SingleLine" id='figure_'4>Figure 4. <i> Growth kinetics of the different strains in the presence of solid sludge from palm oil extraction as the sole source of carbon and energy.</i></div> </div><div class="Text"> <div class="Figure" id="figure_5"><img src="https://article.sciencepublishinggroup.com/article/figure/3841118/3841118_5.png"><p class="article_figures_download"><a href="https://article.sciencepublishinggroup.com/article/figure/3841118/3841118_5.png"><i class="fas fa-arrow-alt-circle-down"></i>Download: Download full-size image</a></p></div><div class="FigureCaption_SingleLine" id='figure_'5>Figure 5. <i> Growth rate of the 8 bacterial strains after 5 days of incubation in the MSM medium supplemented with 4% stock solution of palm oil extraction sludge.</i></div> </div></div><div class="section" id="section4"><div class="Heading1">4. Discussion</div><div class="Text" id="section4_1"><p class='Heading2'>4.1. Evaluation of Hydrogen Potential</p></div><div class="Text">The sludge sample studied for microbiological analysis has a slightly alkaline pH of 7.3 after pH meter stability which lasted nearly 30 minutes. This pH is between 6.5 and 8, the pH interval where biodegradation takes place <div class="references_link" data-target="#references23"><div class="references_detail" style="display: none;" id="references23"><div class="references"><table class="normal_table"><tbody><tr><td>[10]</td><td>Goma-Tchimbakala, J., Obambi, N. J. R, Lebonguy, A. A., Gouolally, T. Biodegradation of PAHs from Used Oil by Consortia of Microorganisms Isolated from Polluted Soils of Brazzaville, Congo. European Journal of Scientific Research. (2020), 155(4), 378–387.</td></tr></tbody></table></div></div>[10]</div>. This pH, close to neutrality, would promote the growth of bacteria and therefore allow the biodegradation of fatty acids to take place efficiently. These results are similar to those obtained by Goma-Tchimbakala <i>et al. </i>(2020) who found PH values of 7.02 and 7.32 in two soils polluted by used motor oil <div class="references_link" data-target="#references24"><div class="references_detail" style="display: none;" id="references24"><div class="references"><table class="normal_table"><tbody><tr><td>[10]</td><td>Goma-Tchimbakala, J., Obambi, N. J. R, Lebonguy, A. A., Gouolally, T. Biodegradation of PAHs from Used Oil by Consortia of Microorganisms Isolated from Polluted Soils of Brazzaville, Congo. European Journal of Scientific Research. (2020), 155(4), 378–387.</td></tr></tbody></table></div></div>[10]</div>.</div><div class="Text" id="section4_2"><p class='Heading2'>4.2. Isolation, Purification and Storage of Bacterial Isolates</p></div><div class="Text">The results of the enumeration on nutrient agar obtained for the different dilutions after 48 hours of incubation showed that the sample of palm oil extraction sludge stored at the SOCAPALM landfill contains a fairly significant bacterial load with a value around 12.6.109 CFU/g of sludge. This high bacterial load is believed to be due to the adaptation of bacteria to the environmental conditions of the environment and the use of palm oil sludge as a source of carbon and energy. It has been proven that the development of microorganisms depends on the characteristics of the environment and the nature of the waste found there <div class="references_link" data-target="#references25"><div class="references_detail" style="display: none;" id="references25"><div class="references"><table class="normal_table"><tbody><tr><td>[20]</td><td>Oksfriani, J. S., Risjani, Y. Bacteria as indicators of environmental pollution: Review. International Journal of Ecosystem (2014), 4(6), 251-258.</td></tr></tbody></table></div></div>[20]</div>. In addition, the adaptation of microorganisms is the consequence of three interdependent mechanisms, namely, the induction and/or depression of specific enzymes, genetic mutations and selective enrichment <div class="references_link" data-target="#references26"><div class="references_detail" style="display: none;" id="references26"><div class="references"><table class="normal_table"><tbody><tr><td>[21]</td><td>Lima, S. D. A., Oliveira, F., Golin, R et al. Isolation and characterization of hydrocarbon-degrading bacteria from gas station leaking-contaminated groundwater in the Southern Amazon, Brazi. Brazilian Journal of Biology. (2020), 80(2), 354-361.</td></tr></tbody></table></div></div>[21]</div>. Similar results were found by Selmoun <i>et al.</i> (2016) from oil drilling waste sloughs, and by Benyahia and Ayadi (2012) from a site polluted by oil <div class="references_link" data-target="#references27"><div class="references_detail" style="display: none;" id="references27"><div class="references"><table class="normal_table"><tbody><tr><td>[15]</td><td>Selmoun, M., Benkhebcheche, D. E. Contribution to the study of the biodegradation of hydrocarbons in drilling muds by bacteria producing biosurfactants. Document of the end of Master cycle for obtaining a Master’s degree in Microbial Ecology; (2016), 77p.</td></tr><tr><td>[22]</td><td>Benyahia, D., Ayadi, M. Characterization of microbial flora of waste sludge from RTC-SONATRACH oil tanks. Thesis for obtaining the state engineer diploma in Environmental Biological Sciences; (2012), 53p.</td></tr></tbody></table></div></div>[15, 22]</div>. Also, Lebonguy (2019) counted 1.53.10<sup>7</sup> CFU/g of soil in the polluted soil of a fuel distribution station in Pointe-Noire <div class="references_link" data-target="#references28"><div class="references_detail" style="display: none;" id="references28"><div class="references"><table class="normal_table"><tbody><tr><td>[23]</td><td>Lebonguy, A. A. Characterization of microorganisms isolated from soil polluted by hydrocarbons and diesel from the bottom of a tank. Unique doctoral thesis, Marien NGouabi University, (2019). 183p.</td></tr></tbody></table></div></div>[23]</div>.</div><div class="Text">Based on all the cultural characteristics, 31 bacteria were isolated and purified from the sample of solid sludge from palm oil extraction. These results show a high culturable bacterial diversity in this waste. This high diversity could be explained by the easier adaptation of bacteria to the carbon source contained in this waste (fatty acids), which are less recalcitrant than other pollutants such as polycyclic aromatic hydrocarbons. These results are in agreement with those obtained by Djien <i>et al.</i> (2021) in soil polluted by liquid effluents from an artisanal palm oil extraction factory <div class="references_link" data-target="#references29"><div class="references_detail" style="display: none;" id="references29"><div class="references"><table class="normal_table"><tbody><tr><td>[24]</td><td>Djien, N. F., Noubou, T. D., Fobasso, T. R et al. Isolation of Bacteria with Purifying Potential and Application in the Treatment of Effluents from an Artisanal Palm Oil Mill in the Littoral Region of Cameroon. Journal of Environmental Protection. (2021), 12, 462-471.</td></tr></tbody></table></div></div>[24]</div>. Furthermore, other authors such as Ndzobo <i>et al.</i> (2022) after the similar study carried out on the isolation, screening and biochemical identification of bacteria with purifying potential in soil polluted by effluents from a paint manufacturing industry, isolated and purified 35 bacterial isolates <div class="references_link" data-target="#references30"><div class="references_detail" style="display: none;" id="references30"><div class="references"><table class="normal_table"><tbody><tr><td>[18]</td><td>Ndzobo, N. E. J., Tavea, M. F., Bella, J et al. Isolation, Screening and Biochemical Identification of Bacteria with Purifying Potential in the Effluents of a Paint Manufacturing Industry. Frontiers in Environmental Microbiology. 8(1), (2022), pp. 6-12. <p class="doi_link"><a href="https://doi.org/10.11648/j.fem.20220801.12" target="_blank" class="normal_link">https://doi.org/10.11648/j.fem.20220801.12<i class="fas fa-external-link-alt"></i></a></p><div class="view_article"><a href="https://doi.org/10.11648/j.fem.20220801.12" target="_blank" class="normal_link">View Article<i class="fas fa-external-link-alt"></i></a></div></td></tr></tbody></table></div></div>[18]</div>.</div><div class="Text" id="section4_3"><p class='Heading2'>4.3. Cultural and Cellular Characterization of Bacterial Isolates</p></div><div class="Text">All the macroscopic and microscopic characteristics studied on the 31 isolates confirm the significant bacterial diversity present in the sludge sample, already shown by the isolation and purification of the colonies. These cultural and cellular characteristics of the isolates allow us to have a first idea of the type of bacteria. All 31 bacterial isolates are bacilli, including 12 Gram+ bacilli and 19 Gram- bacilli. According to the literature, bacteria from the bacilli group are the most involved in the biodegradation of pollutants. These results corroborate those of Goma-Tchimbakala <i>et al.</i> (2020) who found that the bacteria isolated from soil polluted by polycyclic aromatic hydrocarbons all belonged to the <i>Bacillus</i> genus <div class="references_link" data-target="#references31"><div class="references_detail" style="display: none;" id="references31"><div class="references"><table class="normal_table"><tbody><tr><td>[10]</td><td>Goma-Tchimbakala, J., Obambi, N. J. R, Lebonguy, A. A., Gouolally, T. Biodegradation of PAHs from Used Oil by Consortia of Microorganisms Isolated from Polluted Soils of Brazzaville, Congo. European Journal of Scientific Research. (2020), 155(4), 378–387.</td></tr></tbody></table></div></div>[10]</div>. Also Xingjian <i>et al.</i> (2018) found that among bacteria isolated from oil-polluted soil in China, most were bacilli <div class="references_link" data-target="#references32"><div class="references_detail" style="display: none;" id="references32"><div class="references"><table class="normal_table"><tbody><tr><td>[25]</td><td>Xingjian, X., Wenming, L., Shuhua, T. et al. Petroleum hydrocarbon-degrading bacteria for the remediation of oil pollution under aerobic conditions: A perspective analysis. Front Microbiol. (2018), 9, 2885. <p class="doi_link"><a href="https://doi.org/10.3389/fmicb.2018.02885" target="_blank" class="normal_link">https://doi.org/10.3389/fmicb.2018.02885<i class="fas fa-external-link-alt"></i></a></p><div class="view_article"><a href="https://doi.org/10.3389/fmicb.2018.02885" target="_blank" class="normal_link">View Article<i class="fas fa-external-link-alt"></i></a></div></td></tr></tbody></table></div></div>[25]</div>.</div><div class="Text">The hierarchical classification of isolates obtained on the basis of cultural characteristics made it possible to distinguish two large groups of bacteria. Group 1 consists of 19 bacterial isolates which are grouped into three subgroups. Group 2 consists of 12 bacterial isolates which are also grouped into three subgroups. The grouping of the isolates into several groups further shows the existence of the high diversity within the isolates in the sample. Similar results were found by Benadjila (2017) who obtained a significant diversity of bacteria in soil polluted by hydrocarbons <div class="references_link" data-target="#references33"><div class="references_detail" style="display: none;" id="references33"><div class="references"><table class="normal_table"><tbody><tr><td>[26]</td><td>Benadjila, Isolation and identification of bacteria from soil polluted by hydrocarbons and phenol biodegradation test. Thesis for obtaining the Master's degree in Biological Science; (2017), 53p.</td></tr></tbody></table></div></div>[26]</div>.</div><div class="Text" id="section4_4"><p class='Heading2'>4.4. Biochemical Characterization of Bacterial Isolates</p></div><div class="Text" id="section4_4_1"><p class='Heading3'>4.4.1. Characterization of Gram+ Bacteria</p></div><div class="Text">All the cultural, cellular and biochemical characteristics led to the identification of bacterial isolates BI1, BI2, BI3, BI4, BI8, BI14, BI15, BI16, BI18, BI19, BI21 and BI26 as belonging to the genus <i>Bacillus sp</i>. This result could be justified by the fact that bacteria of the genus <i>Bacillus sp</i>. are a group of bacteria very involved in the natural biodegradation of pollutants. These results are in agreement with those of Benyahia and Ayadi (2012) who found during their work on the characterization of the microbial flora of waste sludge from RTC-SONATRACH oil tanks that <i>Bacillus sp</i>. represented 30% of all microorganisms isolated <div class="references_link" data-target="#references34"><div class="references_detail" style="display: none;" id="references34"><div class="references"><table class="normal_table"><tbody><tr><td>[22]</td><td>Benyahia, D., Ayadi, M. Characterization of microbial flora of waste sludge from RTC-SONATRACH oil tanks. Thesis for obtaining the state engineer diploma in Environmental Biological Sciences; (2012), 53p.</td></tr></tbody></table></div></div>[22]</div>. These results also corroborate those obtained by Djien <i>et al.</i> (2021) who found, during their studies on the isolation of bacteria with purifying potential and application to the treatment of effluents from an artisanal palm oil mill in the coastal region of Cameroon, bacteria of the genus <i>Bacillus sp </i><div class="references_link" data-target="#references35"><div class="references_detail" style="display: none;" id="references35"><div class="references"><table class="normal_table"><tbody><tr><td>[24]</td><td>Djien, N. F., Noubou, T. D., Fobasso, T. R et al. Isolation of Bacteria with Purifying Potential and Application in the Treatment of Effluents from an Artisanal Palm Oil Mill in the Littoral Region of Cameroon. Journal of Environmental Protection. (2021), 12, 462-471.</td></tr></tbody></table></div></div>[24]</div>.</div><div class="Text" id="section4_4_2"><p class='Heading3'>4.4.2. Characterization of Gram- Bacteria</p></div><div class="Text">The results of the cultural, cellular and biochemical characteristics led to the identification of bacterial isolates BI6, BI7, BI9, BI12, BI20, BI22, BI27, BI28, BI29 and BI30 as belonging to the genus <i>Pseudomonas sp</i>. However, these characters led to the identification of bacterial isolates BI5, BI10, BI11, BI13, BI23, BI24, BI25 and BI31 as belonging to the species <i>Proteus mirabilis</i> and isolate BI17 as belonging to the species <i>Klebsiella pneumoniae</i>. The presence of these species in polluted environments is due to their ability to tolerate and use pollutants as a source of carbon and energy <div class="references_link" data-target="#references36"><div class="references_detail" style="display: none;" id="references36"><div class="references"><table class="normal_table"><tbody><tr><td>[27]</td><td>Liang, L., Song, X., Kong, J et al. Anaerobic biodegradation of high-molecular-weight polycyclic aromatic hydrocarbons by a facultative anaerobe Pseudomonas sp. JP1. Biodegradation, (2014), 25, 825-833. <p class="doi_link"><a href="https://doi.org/10.1007/s10532-014-9702-5" target="_blank" class="normal_link">https://doi.org/10.1007/s10532-014-9702-5<i class="fas fa-external-link-alt"></i></a></p><div class="view_article"><a href="https://doi.org/10.1007/s10532-014-9702-5" target="_blank" class="normal_link">View Article<i class="fas fa-external-link-alt"></i></a></div></td></tr></tbody></table></div></div>[27]</div>. Most of these strains have already been identified in environments polluted by hydrocarbons <div class="references_link" data-target="#references37"><div class="references_detail" style="display: none;" id="references37"><div class="references"><table class="normal_table"><tbody><tr><td>[10]</td><td>Goma-Tchimbakala, J., Obambi, N. J. R, Lebonguy, A. A., Gouolally, T. Biodegradation of PAHs from Used Oil by Consortia of Microorganisms Isolated from Polluted Soils of Brazzaville, Congo. European Journal of Scientific Research. (2020), 155(4), 378–387.</td></tr><tr><td>[27]</td><td>Liang, L., Song, X., Kong, J et al. Anaerobic biodegradation of high-molecular-weight polycyclic aromatic hydrocarbons by a facultative anaerobe Pseudomonas sp. JP1. Biodegradation, (2014), 25, 825-833. <p class="doi_link"><a href="https://doi.org/10.1007/s10532-014-9702-5" target="_blank" class="normal_link">https://doi.org/10.1007/s10532-014-9702-5<i class="fas fa-external-link-alt"></i></a></p><div class="view_article"><a href="https://doi.org/10.1007/s10532-014-9702-5" target="_blank" class="normal_link">View Article<i class="fas fa-external-link-alt"></i></a></div></td></tr><tr><td>[28]</td><td>Benchouk, A., chibani Petroleum-hydrocarbons biodegradationby pseudomanas strains isolated from hydrocarbon-contaminated soil. J. fundam. Appl. Sci. (2017), 9(2), 713-726.</td></tr><tr><td>[29]</td><td>Lebonguy, A. A., Goma-Tchimbakala, J., Miambi, E., Keleke, S. Isolation and caracterisation of petroleum product emulsifing pseudomonas strains from a generation set fuel tank. African journal of microbiology reasearch, (2017), 11(22), 920-926. <p class="doi_link"><a href="https://doi.org/10.5897/AJMR2016.809" target="_blank" class="normal_link">https://doi.org/10.5897/AJMR2016.809<i class="fas fa-external-link-alt"></i></a></p><div class="view_article"><a href="https://doi.org/10.5897/AJMR2016.809" target="_blank" class="normal_link">View Article<i class="fas fa-external-link-alt"></i></a></div></td></tr></tbody></table></div></div>[10, 27-29]</div>. Liang <i>et al.</i> (2014) also identified a strain of <i>Pseudomonas sp</i>. JP1 of polluted sediments from Shantou port in China <div class="references_link" data-target="#references38"><div class="references_detail" style="display: none;" id="references38"><div class="references"><table class="normal_table"><tbody><tr><td>[27]</td><td>Liang, L., Song, X., Kong, J et al. Anaerobic biodegradation of high-molecular-weight polycyclic aromatic hydrocarbons by a facultative anaerobe Pseudomonas sp. JP1. Biodegradation, (2014), 25, 825-833. <p class="doi_link"><a href="https://doi.org/10.1007/s10532-014-9702-5" target="_blank" class="normal_link">https://doi.org/10.1007/s10532-014-9702-5<i class="fas fa-external-link-alt"></i></a></p><div class="view_article"><a href="https://doi.org/10.1007/s10532-014-9702-5" target="_blank" class="normal_link">View Article<i class="fas fa-external-link-alt"></i></a></div></td></tr></tbody></table></div></div>[27]</div>.</div><div class="Text" id="section4_5"><p class='Heading2'>4.5. Biodegradability Test of Palm Oil and Extraction Sludge Using Bacterial Isolates</p></div><div class="Text" id="section4_5_1"><p class='Heading3'>4.5.1. Palm Oil Biodegradability Test</p></div><div class="Text">The results of the palm oil biodegradation test show that all 31 bacterial isolates tested grew on the M2 medium supplemented with 2% palm oil and devoid of yeast extract after 48 hours with a difference in the load. Unlike, in boxes containing M2 medium with 2% palm oil and 1g/L of yeast extract, growth is obtained after 24 hours with a heavier load. These observations show that all bacteria isolated from palm oil extraction sludge can use the palm oil contained in this sludge as a sole source of carbon and energy and therefore can degrade this pollutant. The presence of a second carbon source leads to more rapid growth of these bacteria which can effectively degrade this waste. Similar results were found by Selmoun <i>et al.</i> (2016) during their work on the contribution to the study of the biodegradation of hydrocarbons in drilling muds by bacteria producing biosurfactants <div class="references_link" data-target="#references39"><div class="references_detail" style="display: none;" id="references39"><div class="references"><table class="normal_table"><tbody><tr><td>[15]</td><td>Selmoun, M., Benkhebcheche, D. E. Contribution to the study of the biodegradation of hydrocarbons in drilling muds by bacteria producing biosurfactants. Document of the end of Master cycle for obtaining a Master’s degree in Microbial Ecology; (2016), 77p.</td></tr></tbody></table></div></div>[15]</div>. Benyahia and Ayadi (2012) found during their work on the characterization of the microbial flora of waste sludge from RTC-SONATRACH oil tanks that half of the strains isolated had good growth on all the hydrocarbons tested as carbon substrate <div class="references_link" data-target="#references40"><div class="references_detail" style="display: none;" id="references40"><div class="references"><table class="normal_table"><tbody><tr><td>[22]</td><td>Benyahia, D., Ayadi, M. Characterization of microbial flora of waste sludge from RTC-SONATRACH oil tanks. Thesis for obtaining the state engineer diploma in Environmental Biological Sciences; (2012), 53p.</td></tr></tbody></table></div></div>[22]</div>.</div><div class="Text" id="section4_5_2"><p class='Heading3'>4.5.2. Biodegradability Test of Solid Sludge from Palm Oil Extraction</p></div><div class="Text">The growth kinetics measured after 7 days for the 8 isolates in the presence of solid sludge from palm oil extraction as the sole source of carbon and energy showed 5 growth phases. An acceleration phase between day 0 and day 1 during which there is an acceleration in the rate of bacterial division. A slowdown phase between D1 and D3 during which there is a slowdown in the rate of bacterial division. A phase of exponential growth between D3 and D5 during which the bacteria are in their optimum physiological and metabolic state with maximum division. A phase of decline between D5 and D6 where growing conditions become unfavorable. Then a second acceleration phase between D6 and D7. The absence of the latency phase shows that the latency time was very short. These results demonstrate a more or less rapid adaptation of the isolates to the carbon source used and the rapid establishment of the enzymatic machinery necessary for the biodegradation of this waste. The resumption of the acceleration of growth between day 6 and day 7 would be due to the fact that certain bacteria have adapted to consume the waste generated or their dead congeners. The growth curves in <a class='a_link' href='javascript:;' data='figure_4'>Figure 4</a> show that the speed of growth and therefore that of the degradation of palm oil extraction sludge is very important during the first 5 days. This growth is continuous during the first 5 days of incubation with a maximum number of microorganisms on the 5th day of incubation in all isolates. The optical density results show that all bacterial isolates tested have the ability to use palm oil sludge as the sole source of carbon and energy, but to different degrees. These results could be justified by the fact that the initial attack on the carbon source differs from one isolate to another and, moreover, by the individual growth characteristics of each isolate <div class="references_link" data-target="#references41"><div class="references_detail" style="display: none;" id="references41"><div class="references"><table class="normal_table"><tbody><tr><td>[30]</td><td>Guermouche, M. A. Molecular characterization of bacteria involved in the biodegradation of hydrocarbons. Doctoral thesis in biotechnology, University of Oran, (2014), 153p.</td></tr></tbody></table></div></div>[30]</div>. Bacterial isolates BI5, BI31 and BI29 exhibit the highest growth rates, respectively. Isolate BI9 presents the lowest growth rate. We also note that most of the isolates show good growth over the entire duration of the incubation, their growth appears stationary. However, we observed a drop in growth between day 5 and day 6, then a recovery between day 6 and day 7 in all isolates. These results corroborate those obtained by Guermouche (2014) who, during their work on the molecular characterization of bacteria involved in the biodegradation of hydrocarbons, found that certain bacterial strains had more capacity to degrade oil compared to others <div class="references_link" data-target="#references42"><div class="references_detail" style="display: none;" id="references42"><div class="references"><table class="normal_table"><tbody><tr><td>[30]</td><td>Guermouche, M. A. Molecular characterization of bacteria involved in the biodegradation of hydrocarbons. Doctoral thesis in biotechnology, University of Oran, (2014), 153p.</td></tr></tbody></table></div></div>[30]</div>.</div><div class="Text">The results of the evolution of the biomass of the cell suspension of each isolate inoculated in the liquid MSM medium supplemented with 4% stock solution of palm oil extraction sludge after 7 days of incubation showed a variation of the biomass of the isolates between 16 and 187.2%. Bacterial isolates BI2, BI5, BI31 and BI10 showed the highest growth rates and isolates BI29 and BI22 the lowest growth rates. Seventy-five percent of the isolates had a growth rate greater than 50%. These results corroborate those of Ndzobo <i>et al.</i> (2022) who found during their studies on the isolation, screening and biochemical identification of bacteria with purifying potential in a soil polluted by the effluents of a paint manufacturing industry in Cameroon that the isolates IS20, IS59, IS19; isolates IS25, IS35, IS45, IS37, IS55 and isolates IS06, IS08, IS41, IS43, IS15, IS30, IS49 and IS10 had the growth rates of 37%, 50% and 30%, respectively <div class="references_link" data-target="#references43"><div class="references_detail" style="display: none;" id="references43"><div class="references"><table class="normal_table"><tbody><tr><td>[18]</td><td>Ndzobo, N. E. J., Tavea, M. F., Bella, J et al. Isolation, Screening and Biochemical Identification of Bacteria with Purifying Potential in the Effluents of a Paint Manufacturing Industry. Frontiers in Environmental Microbiology. 8(1), (2022), pp. 6-12. <p class="doi_link"><a href="https://doi.org/10.11648/j.fem.20220801.12" target="_blank" class="normal_link">https://doi.org/10.11648/j.fem.20220801.12<i class="fas fa-external-link-alt"></i></a></p><div class="view_article"><a href="https://doi.org/10.11648/j.fem.20220801.12" target="_blank" class="normal_link">View Article<i class="fas fa-external-link-alt"></i></a></div></td></tr></tbody></table></div></div>[18]</div>.</div></div><div class="section" id="section5"><div class="Heading1">5. Conclusion</div><div class="Text">This work aimed to isolate and characterize indigenous bacteria with biodegradation capacities in sludge from palm oil extraction. It appears that 31 bacteria were isolated and purified from the sludge sample, including 12 <i>Bacillus sp</i>, 10 <i>Pseudomonas sp</i>, 8 <i>Proteus mirabilis</i> and 1 <i>Klebsiella pneumoniae</i>. All 31 bacterial isolates tested for their ability to use palm oil as the sole carbon and energy source grew in the culture medium in the presence of palm oil as the sole carbon and energy source. All the top 8 isolates selected from the palm oil biodegradability test and tested for their ability to degrade solid sludge from palm oil extraction showed very rapid adaptation to the source of carbon, a very high speed and growth rate. Of all these 8 bacterial isolates, bacterial isolates BI2, BI5, BI31, BI10 and BI9 showed the highest growth rates. These isolates could be used to constitute consortia of microorganisms that can be used in the treatment of waste generated by palm oil extraction.</div></div><div class="section" id="section6"><div class="Heading1">Abbreviations</div><div class="Text">ADH: Arginine Dihydrolase</div><div class="Text">AMY: Amygdalin</div><div class="Text">ARA: Arabinose</div><div class="Text">BAG: Sucrose</div><div class="Text">BI: Bacterial Isolate</div><div class="Text">CIT: Citrate</div><div class="Text">GEL: Gelatin</div><div class="Text">GLU: Glucose</div><div class="Text">H<sub>2</sub>S: Hydrogen Sulfide</div><div class="Text">IND: Indole</div><div class="Text">INO: Inositol</div><div class="Text">LDC: Lysine Décarboxylase</div><div class="Text">MAN: Mannose</div><div class="Text">MEL: Melibiosis</div><div class="Text">OD: Optical Density</div><div class="Text">ODC: Ornithine Décarboxylase</div><div class="Text">ONPG: O-nitrophényl- D-galactopyranoside</div><div class="Text">OX: Oxydase</div><div class="Text">RHA: Rhamnose</div><div class="Text">SOR: Sorbitol</div><div class="Text">TDA: Tryptophan Deaminase</div><div class="Text">URE: Urea</div><div class="Text">VP: Voges-Proskauer</div></div><div class="section" id="section7"><div class="Heading1">Acknowledgments</div><div class="Text">The authors thank the Laboratory of the Department of Plant Biology of the Faculty of Science of the University of Douala for some analyzes that have been done.</div></div><div class="section" id="section8"><div class="Heading1">Author Contributions</div><div class="Text">Tassong Saah Denis: Data curation, Software, Funding acquisition, Investigation, Methodology, Writing – original draft</div><div class="Text">Simo Claude: Conceptualization, Software, Formal Analysis, Validation, Visualization, Writing – original draft, Project administration, Writing – review & editing</div><div class="Text">Taffouo Vctor Désiré: Supervision, Validation</div></div><div class="section" id="section9"><div class="Heading1">Conflicts of Interest</div><div class="Text">The authors declare no conflicts of interest.</div></div> <div class="section" id="references"> <div class="Heading1">References</div> <div class="references"> <table class="normal_table"> <tbody> <tr id="ref_1" class="References"> <td>[1] </td> <td style="word-break: break-word;"> Jacquemard, J. 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Doctoral thesis in biotechnology, University of Oran, (2014), 153p. </td> </tr> </tbody> </table> </div> </div> <div class="section" id="cite_this_article" style="margin-bottom: 20px;"> <div class="Heading1">Cite This Article</div> <div class="cite_article"> <div class="mt-tabpage" js-tab="2"> <div class="mt-tabpage-title"> <a class="mt-tabpage-item mt-tabpage-item-cur">Plain Text</a> <a class="mt-tabpage-item">BibTeX</a> <a class="mt-tabpage-item">RIS</a> </div> <div class="mt-tabpage-count"> <ul class="mt-tabpage-cont__wrap"> <li class="mt-tabpage-item"> <div class="tab_div"> <div class="cite_type"> <p class="cite_type_item">APA Style</p> <p class="cite_type_info apa-copy-src">Denis, T. S., Claude, S., Désiré, T. V. (2024). Isolation and Characterization of Indigenous Bacteria with Purifying Potential in Solid Palm Oil Extraction Sludge Generated by SOCAPALM-Mbambou. <i>Frontiers in Environmental Microbiology</i>, <i>10</i>(2), 29-44. <a href='https://doi.org/10.11648/j.fem.20241002.11'>https://doi.org/10.11648/j.fem.20241002.11</a></p> <p class="cite_operation"> <span><a class="apa-copy copy-el" data-clipboard-action="copy" data-clipboard-target=".apa-copy-src" href="javascript:;" ><img src="/img/copy_icon.png">Copy</a></span> <span class="line">|</span> <span><a href="javascript:;" onclick="spgCommon.bindDownloadDataFromEl("10.11648.j.fem.20241002.11.apa.txt", '.apa-copy-src')"><img src="/img/download_icon.png">Download</a></span> </p> </div> <div class="cite_type"> <p class="cite_type_item">ACS Style</p> <p class="cite_type_info acs-copy-src">Denis, T. S.; Claude, S.; Désiré, T. V. Isolation and Characterization of Indigenous Bacteria with Purifying Potential in Solid Palm Oil Extraction Sludge Generated by SOCAPALM-Mbambou. <i>Front. Environ. Microbiol.</i> <b>2024</b>, <i>10</i>(2), 29-44. <a href='https://doi.org/10.11648/j.fem.20241002.11'>doi: 10.11648/j.fem.20241002.11</a></p> <p class="cite_operation"> <span><a class="acs-copy copy-el" data-clipboard-action="copy" data-clipboard-target=".acs-copy-src" href="javascript:;"><img src="/img/copy_icon.png">Copy</a></span> <span class="line">|</span> <span><a href="javascript:;" onclick="spgCommon.bindDownloadDataFromEl("10.11648.j.fem.20241002.11.acs.txt", '.acs-copy-src')"><img src="/img/download_icon.png">Download</a></span> </p> </div> <div class="cite_type"> <p class="cite_type_item">AMA Style</p> <p class="cite_type_info ama-copy-src">Denis TS, Claude S, Désiré TV. Isolation and Characterization of Indigenous Bacteria with Purifying Potential in Solid Palm Oil Extraction Sludge Generated by SOCAPALM-Mbambou. <i>Front Environ Microbiol</i>. 2024;10(2):29-44. <a href='https://doi.org/10.11648/j.fem.20241002.11'>doi: 10.11648/j.fem.20241002.11</a></p> <p class="cite_operation"> <span><a class="ama-copy copy-el" data-clipboard-action="copy" data-clipboard-target=".ama-copy-src" href="javascript:;"><img src="/img/copy_icon.png">Copy</a></span> <span class="line">|</span> <span><a href="javascript:;" onclick="spgCommon.bindDownloadDataFromEl("10.11648.j.fem.20241002.11.ama.txt", '.ama-copy-src')"><img src="/img/download_icon.png">Download</a></span> </p> </div> </div> </li> <li class="mt-tabpage-item"> <div class="tab_div"> <pre _ngcontent-anj-c150="" class="text ris-text bib-copy-src main_content_citetext">@article{10.11648/j.fem.20241002.11, author = {Tassong Saah Denis and Simo Claude and Taffouo Victor Désiré}, title = {Isolation and Characterization of Indigenous Bacteria with Purifying Potential in Solid Palm Oil Extraction Sludge Generated by SOCAPALM-Mbambou }, journal = {Frontiers in Environmental Microbiology}, volume = {10}, number = {2}, pages = {29-44}, doi = {10.11648/j.fem.20241002.11}, url = {https://doi.org/10.11648/j.fem.20241002.11}, eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20241002.11}, abstract = {The oil palm industry contributes significantly to the economic development of producing countries such as Cameroon. Unfortunately, the exploitation of palm oil constitutes a source of environmental pollution due to the production of enormous quantities of waste during its extraction process, including solid sludge generating greenhouse gases which contribute to global warming climatic. All this leads to the search for alternatives which consists of isolating and characterizing indigenous bacteria with biodegradation capacities in sludge from palm oil extraction. The pH and bacterial counts were determined by the potentiometric method and the decimal dilution technique, respectively. The isolated bacteria were identified by their cultural, cellular and biochemical characteristics. In addition, the identification of Gram- bacteria was further explored by the API 20 E gallery. The palm oil biodegradability test was carried out on M2 medium supplemented with 2% palm oil. The solid sludge biodegradability test was carried out on liquid MSM medium supplemented with 4% sludge stock solution. The results showed that the sludge sample had a slightly alkaline pH of 7.3. A bacterial load of around 109 CFU/g of soil was counted. Thirty-one bacterial strains were isolated and purified, including 12 Bacillus sp, 10 Pseudomonas sp, 8 Proteus mirabilis and 1 Klebsiella pneumoniae. All isolates tested for their ability to degrade palm oil or solid sludge grew in culture media with palm oil or solid sludge as the sole source of carbon and energy but with a difference in load. Thus, isolates BI2, BI5, BI31, BI10 and BI 9 showed the highest degradation capacities. These isolates could be used to constitute consortia of microorganisms that can be used in the treatment of waste generated by palm oil extraction. }, year = {2024} } </pre> <p class="cite_operation"> <span><a class="bib-copy copy-el" data-clipboard-action="copy" data-clipboard-target=".bib-copy-src" href="javascript:;"><img src="/img/copy_icon.png">Copy</a></span> <span class="line">|</span> <span><a href="javascript:;" onclick="spgCommon.bindDownloadDataFromEl("10.11648.j.fem.20241002.11.bib", '.bib-copy-src')"><img src="/img/download_icon.png">Download</a></span> </p> </div> </li> <li class="mt-tabpage-item"> <div class="tab_div"> <pre _ngcontent-anj-c150="" class="text ris-text ris-copy-src main_content_citetext">TY - JOUR T1 - Isolation and Characterization of Indigenous Bacteria with Purifying Potential in Solid Palm Oil Extraction Sludge Generated by SOCAPALM-Mbambou AU - Tassong Saah Denis AU - Simo Claude AU - Taffouo Victor Désiré Y1 - 2024/04/17 PY - 2024 N1 - https://doi.org/10.11648/j.fem.20241002.11 DO - 10.11648/j.fem.20241002.11 T2 - Frontiers in Environmental Microbiology JF - Frontiers in Environmental Microbiology JO - Frontiers in Environmental Microbiology SP - 29 EP - 44 PB - Science Publishing Group SN - 2469-8067 UR - https://doi.org/10.11648/j.fem.20241002.11 AB - The oil palm industry contributes significantly to the economic development of producing countries such as Cameroon. Unfortunately, the exploitation of palm oil constitutes a source of environmental pollution due to the production of enormous quantities of waste during its extraction process, including solid sludge generating greenhouse gases which contribute to global warming climatic. All this leads to the search for alternatives which consists of isolating and characterizing indigenous bacteria with biodegradation capacities in sludge from palm oil extraction. The pH and bacterial counts were determined by the potentiometric method and the decimal dilution technique, respectively. The isolated bacteria were identified by their cultural, cellular and biochemical characteristics. In addition, the identification of Gram- bacteria was further explored by the API 20 E gallery. The palm oil biodegradability test was carried out on M2 medium supplemented with 2% palm oil. The solid sludge biodegradability test was carried out on liquid MSM medium supplemented with 4% sludge stock solution. The results showed that the sludge sample had a slightly alkaline pH of 7.3. A bacterial load of around 109 CFU/g of soil was counted. Thirty-one bacterial strains were isolated and purified, including 12 Bacillus sp, 10 Pseudomonas sp, 8 Proteus mirabilis and 1 Klebsiella pneumoniae. All isolates tested for their ability to degrade palm oil or solid sludge grew in culture media with palm oil or solid sludge as the sole source of carbon and energy but with a difference in load. Thus, isolates BI2, BI5, BI31, BI10 and BI 9 showed the highest degradation capacities. These isolates could be used to constitute consortia of microorganisms that can be used in the treatment of waste generated by palm oil extraction. VL - 10 IS - 2 ER - </pre> <p class="cite_operation"> <span><a class="ris-copy copy-el" data-clipboard-action="copy" data-clipboard-target=".ris-copy-src" href="javascript:;"><img src="/img/copy_icon.png">Copy</a></span> <span class="line">|</span> <span><a href="javascript:;" onclick="spgCommon.bindDownloadDataFromEl("10.11648.j.fem.20241002.11.ris", '.ris-copy-src')"><img src="/img/download_icon.png">Download</a></span> </p> </div> </li> </ul> </div> </div> </div> </div> <div class="section" id="author_information" style="margin-top: 0px; margin-bottom: 50px;"> <div class="Heading1">Author Information</div> <div class="author_information"> <ul> <li> <div class="author_info"> <p class="article_author_name">Tassong Saah Denis</p> <p class="Affiliation">Laboratory of Plant Biology, Department of Plant Biology, Faculty of Science, University of Douala, Douala, Cameroon</p> <div class="contact_info"> <input type="hidden" class="person-id" value="10687509"> <p><img src="/img/email_icon.png"><a href="javascript:;" class="EmailAddress btn-email" data-toggle="modal" data-target="#emailModalScrollable">Contact Email</a></p> <p><img src="/img/orcid_icon.png"><a href="http://orcid.org/0009-0008-0637-6793" target="_blank" class="normal_link">http://orcid.org/0009-0008-0637-6793<i class="fas fa-external-link-alt"></i></a> </p> </div> </div> </li> <li> <div class="author_info"> <p class="article_author_name">Simo Claude</p> <p class="Affiliation">Laboratory of Plant Biology, Department of Plant Biology, Faculty of Science, University of Douala, Douala, Cameroon; Laboratory of Plant Physiology, Department of Biological Sciences, Higher Teacher’s Training College, University of Yaoundé I, Yaounde, Cameroon</p> <div class="contact_info"> <input type="hidden" class="person-id" value="10687510"> <p><img src="/img/email_icon.png"><a href="javascript:;" class="EmailAddress btn-email" data-toggle="modal" data-target="#emailModalScrollable">Contact Email</a></p> <p><img src="/img/orcid_icon.png"><a href="http://orcid.org/0009-0008-7800-4761" target="_blank" class="normal_link">http://orcid.org/0009-0008-7800-4761<i class="fas fa-external-link-alt"></i></a> </p> </div> </div> </li> <li> <div class="author_info"> <p class="article_author_name">Taffouo Victor Désiré</p> <p class="Affiliation">Laboratory of Plant Biology, Department of Plant Biology, Faculty of Science, University of Douala, Douala, Cameroon</p> <div class="contact_info"> <input type="hidden" class="person-id" value="10687511"> <p><img src="/img/email_icon.png"><a href="javascript:;" class="EmailAddress btn-email" data-toggle="modal" data-target="#emailModalScrollable">Contact Email</a></p> <p><img src="/img/orcid_icon.png"><a href="http://orcid.org/0000-0002-3745-6875" target="_blank" class="normal_link">http://orcid.org/0000-0002-3745-6875<i class="fas fa-external-link-alt"></i></a> </p> </div> </div> </li> </ul> </div> </div> </div> </div> <div class="article_position right"> <div class="article_content_right"> <div class="toggle-close clearfix"><i class="fas fa-times"></i></div> <input type="hidden" id="articleID" value="10087896"> <div class="right_content_btn download_right"> <a href="https://article.sciencepublishinggroup.com/pdf/j.fem.20241002.11" target="_blank" onclick="downLoadArticle(10087896, "https:\/\/w.sciencepublishinggroup.com\/", '.spanDownloads', "https:\/\/article.sciencepublishinggroup.com\/", "pdf\/j.fem.20241002.11", true)" > <i class="fas fa-file-pdf"></i>Download PDF </a> </div> <div id="slider" class="article_tab">  <div class="controls-center"> <div id="slider_controls"> <ul id="slider_nav" class="clearfix"> <li><a href="javascript:;"><i class="fas fa-bars"></i>Sections</a></li> <li><a href="javascript:;"><i class="fas fa-image"></i>Figures</a></li> <li><a href="javascript:;"><i class="fas fa-border-all"></i>Tables</a></li> </ul> </div> </div>  <div class="article_tab_item" style="background-color: white;"> <div id="slideshow"> <div class="slider-item"> <ul class="section_ul"> <li class="section_nav"> <a href="javascript:;" data="abstract">Abstract</a> </li> <li class="section_nav"> <a href="javascript:;" data="keywords">Keywords</a> </li> <li class="section_nav"> <p class="document_section">Document Sections</p> <ol class="document_section_ol"> <script> var sectionOfBodyList = [{"id":"1","caption":"Introduction","level":1},{"id":"2","caption":"Materials and Methods","level":1},{"id":"2_1","caption":"2.1. Materials","level":2},{"id":"2_2","caption":"2.2. Methods","level":2},{"id":"2_2_1","caption":"2.2.1. Evaluation of Hydrogen Potential","level":3},{"id":"2_2_2","caption":"2.2.2. Isolation, Purification and Storage of Bacterial Isolates","level":3},{"id":"2_2_3","caption":"2.2.3. Cultural and Cellular Characterization of Isolates","level":3},{"id":"(i)","caption":"(i). Macroscopic Appearance","level":2},{"id":"(ii)","caption":"(ii). Microscopic Appearance","level":2},{"id":"2_2_4","caption":"2.2.4. Biochemical Characterization of Isolates","level":3},{"id":"2_2_5","caption":"2.2.5. Biodegradability Test of Palm Oil and Extraction Sludge Using Isolates","level":3},{"id":"(i)","caption":"(i). Palm oil Biodegradability Test","level":2},{"id":"(ii)","caption":"(ii). Biodegradability Test of Solid Sludge from Palm Oil Extraction","level":2},{"id":"2_2_6","caption":"2.2.6. Data Analyzes","level":3},{"id":"3","caption":"Results","level":1},{"id":"3_1","caption":"3.1. Evaluation of Hydrogen Potential","level":2},{"id":"3_2","caption":"3.2. Isolation, Purification and Storage of Bacterial Isolates","level":2},{"id":"3_3","caption":"3.3. Cultural and Cellular Characterization of Bacterial Isolates","level":2},{"id":"3_4","caption":"3.4. Biochemical Characterization of Isolates","level":2},{"id":"3_4_1","caption":"3.4.1. Characterization of Gram+ Bacteria","level":3},{"id":"3_4_2","caption":"3.4.2. Characterization of Gram- Bacteria","level":3},{"id":"3_5","caption":"3.5. Biodegradability Test of Palm Oil and Extraction Sludge Using Bacterial Isolates","level":2},{"id":"3_5_1","caption":"3.5.1. Palm Oil Biodegradability Test","level":3},{"id":"3_5_2","caption":"3.5.2. Biodegradability Test of Solid Sludge from Palm Oil Extraction","level":3},{"id":"4","caption":"Discussion","level":1},{"id":"4_1","caption":"4.1. Evaluation of Hydrogen Potential","level":2},{"id":"4_2","caption":"4.2. Isolation, Purification and Storage of Bacterial Isolates","level":2},{"id":"4_3","caption":"4.3. Cultural and Cellular Characterization of Bacterial Isolates","level":2},{"id":"4_4","caption":"4.4. Biochemical Characterization of Bacterial Isolates","level":2},{"id":"4_4_1","caption":"4.4.1. Characterization of Gram+ Bacteria","level":3},{"id":"4_4_2","caption":"4.4.2. Characterization of Gram- Bacteria","level":3},{"id":"4_5","caption":"4.5. Biodegradability Test of Palm Oil and Extraction Sludge Using Bacterial Isolates","level":2},{"id":"4_5_1","caption":"4.5.1. Palm Oil Biodegradability Test","level":3},{"id":"4_5_2","caption":"4.5.2. Biodegradability Test of Solid Sludge from Palm Oil Extraction","level":3},{"id":"5","caption":"Conclusion","level":1}]; </script> <li class="document_section_nav" data="1"> <a href="javascript:;" data="section1">1. Introduction</a> </li> <li class="document_section_nav" data="2"> <a href="javascript:;" data="section2">2. Materials and Methods</a> </li> <li class="document_section_nav" data="3"> <a href="javascript:;" data="section3">3. Results</a> </li> <li class="document_section_nav" data="4"> <a href="javascript:;" data="section4">4. Discussion</a> </li> <li class="document_section_nav" data="5"> <a href="javascript:;" data="section5">5. Conclusion</a> </li> </ol> <div id="toggleFullOutline" class="toggle_second_nav" onclick="toggleFullOutline()">Show Full Outline<i class="fas fa-caret-down"></i></div> </li> <li class="section_nav"> <a href="javascript:;" data="section6">Abbreviations</a> </li> <li class="section_nav"> <a href="javascript:;" data="section7">Acknowledgments</a> </li> <li class="section_nav"> <a href="javascript:;" data="section8">Author Contributions</a> </li> <li class="section_nav"> <a href="javascript:;" data="section9">Conflicts of Interest</a> </li> <li class="section_nav"> <a href="javascript:;" data="references">References</a> </li> <li class="section_nav"> <a href="javascript:;" data="cite_this_article">Cite This Article</a> </li> <li class="section_nav"> <a href="javascript:;" data="author_information">Author Information</a> </li> </ul> </div> <div class="slider-item overflow-y"> <ul class="figures_ul"> <li> <p class="figure_number"><a href="javascript:;" data="figure_1">Figure 1</a></p> <a href="javascript:;" data="figure_1"> <img src="https://article.sciencepublishinggroup.com/article/figure/3841118/3841118_1.png"> <p class="figure_description">Figure 1. <i> </i><i>Cultural (A, B, C, D) and cellular (E, F, G, H) (Gx100) aspects of the respective isolates BI19, BI4, BI23 and BI12.</i></p> </a> </li> <li> <p class="figure_number"><a href="javascript:;" data="figure_2">Figure 2</a></p> <a href="javascript:;" data="figure_2"> <img src="https://article.sciencepublishinggroup.com/article/figure/3841118/3841118_2.png"> <p class="figure_description">Figure 2. <i> </i><i>Hierarchical classification of bacterial isolates obtained on the basis of cultural characteristics.</i></p> </a> </li> <li> <p class="figure_number"><a href="javascript:;" data="figure_3">Figure 3</a></p> <a href="javascript:;" data="figure_3"> <img src="https://article.sciencepublishinggroup.com/article/figure/3841118/3841118_3.png"> <p class="figure_description">Figure 3. <i> </i><i>Some images of the growth of bacterial isolates on M2 medium with 2% palm oil as the sole carbon source (A, B, C) and on M2 medium supplemented with palm oil plus yeast extract (D, E, F).</i></p> </a> </li> <li> <p class="figure_number"><a href="javascript:;" data="figure_4">Figure 4</a></p> <a href="javascript:;" data="figure_4"> <img src="https://article.sciencepublishinggroup.com/article/figure/3841118/3841118_4.png"> <p class="figure_description">Figure 4. <i> </i><i>Growth kinetics of the different strains in the presence of solid sludge from palm oil extraction as the sole source of carbon and energy.</i></p> </a> </li> <li> <p class="figure_number"><a href="javascript:;" data="figure_5">Figure 5</a></p> <a href="javascript:;" data="figure_5"> <img src="https://article.sciencepublishinggroup.com/article/figure/3841118/3841118_5.png"> <p class="figure_description">Figure 5. <i> </i><i>Growth rate of the 8 bacterial strains after 5 days of i</i><i>n</i><i>cubation in the MSM medium supplemented with 4% stock solution of palm oil extraction sludge.</i></p> </a> </li> </ul> </div> <div class="slider-item overflow-y"> <ul class="table_ul"> <li> <p class="table_number"><a href="javascript:;" data="table_1"><i class="fas fa-border-all"></i>Table 1</a></p> <a href="javascript:;" data="table_1"><p class="table_description">Table 1. <i> Cultural characteristics of bacterial isolates.</i></p></a> </li> <li> <p class="table_number"><a href="javascript:;" data="table_2"><i class="fas fa-border-all"></i>Table 2</a></p> <a href="javascript:;" data="table_2"><p class="table_description">Table 2. <i> Cellular characteristics of bacterial isolates.</i></p></a> </li> <li> <p class="table_number"><a href="javascript:;" data="table_3"><i class="fas fa-border-all"></i>Table 3</a></p> <a href="javascript:;" data="table_3"><p class="table_description">Table 3. <i> Results of oxidase and catalase tests in gram+ bacteria.</i></p></a> </li> <li> <p class="table_number"><a href="javascript:;" data="table_4"><i class="fas fa-border-all"></i>Table 4</a></p> <a href="javascript:;" data="table_4"><p class="table_description">Table 4. <i> Results of the 11 biochemical tests of Gram- bacteria based on the API20E Kit and the oxidase test.</i></p></a> </li> <li> <p class="table_number"><a href="javascript:;" data="table_5"><i class="fas fa-border-all"></i>Table 5</a></p> <a href="javascript:;" data="table_5"><p class="table_description">Table 5. <i> Results of the 10 biochemical tests of Gram- bacteria based on the API20E Kit and the oxidase test.</i></p></a> </li> <li> <p class="table_number"><a href="javascript:;" data="table_6"><i class="fas fa-border-all"></i>Table 6</a></p> <a href="javascript:;" data="table_6"><p class="table_description">Table 6. <i> Test de biodégradabilité de l’huile de palme par les différents isolats bactériens.</i></p></a> </li> </ul> </div> </div> </div> <div class="clr"></div> <div class="clearfix"></div> </div> </div> <div class="tab-nav-shortcut"> 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Isolation and Characterization of Indigenous Bacteria with Purifying Potential in Solid Palm Oil Extraction Sludge Generated by SOCAPALM-Mbambou. <i>Front Environ Microbiol</i>. 2024;10(2):29-44. <a href='https://doi.org/10.11648/j.fem.20241002.11'>doi: 10.11648/j.fem.20241002.11</a></p> <p class="cite_operation"> <span><a class="ama-copy-pop copy-el" data-clipboard-action="copy" data-clipboard-target=".ama-copy-pop-src" href="javascript:;"><img src="/img/copy_icon.png">Copy</a></span> <span class="line">|</span> <span><a href="javascript:;" onclick="spgCommon.bindDownloadDataFromEl("10.11648.j.fem.20241002.11.ama.txt", '.ama-copy-pop-src')"><img src="/img/download_icon.png">Download</a></span> </p> </div> </div> </li> <li class="mt-tabpage-item puple_cite"> <div class="tab_div"> <pre _ngcontent-anj-c150="" class="text ris-text bib-copy-pop-src">@article{10.11648/j.fem.20241002.11, author = {Tassong Saah Denis and Simo Claude and Taffouo Victor Désiré}, title = {Isolation and Characterization of Indigenous Bacteria with Purifying Potential in Solid Palm Oil Extraction Sludge Generated by SOCAPALM-Mbambou }, journal = {Frontiers in Environmental Microbiology}, volume = {10}, number = {2}, pages = {29-44}, doi = {10.11648/j.fem.20241002.11}, url = {https://doi.org/10.11648/j.fem.20241002.11}, eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20241002.11}, abstract = {The oil palm industry contributes significantly to the economic development of producing countries such as Cameroon. Unfortunately, the exploitation of palm oil constitutes a source of environmental pollution due to the production of enormous quantities of waste during its extraction process, including solid sludge generating greenhouse gases which contribute to global warming climatic. All this leads to the search for alternatives which consists of isolating and characterizing indigenous bacteria with biodegradation capacities in sludge from palm oil extraction. The pH and bacterial counts were determined by the potentiometric method and the decimal dilution technique, respectively. The isolated bacteria were identified by their cultural, cellular and biochemical characteristics. In addition, the identification of Gram- bacteria was further explored by the API 20 E gallery. The palm oil biodegradability test was carried out on M2 medium supplemented with 2% palm oil. The solid sludge biodegradability test was carried out on liquid MSM medium supplemented with 4% sludge stock solution. The results showed that the sludge sample had a slightly alkaline pH of 7.3. A bacterial load of around 109 CFU/g of soil was counted. Thirty-one bacterial strains were isolated and purified, including 12 Bacillus sp, 10 Pseudomonas sp, 8 Proteus mirabilis and 1 Klebsiella pneumoniae. All isolates tested for their ability to degrade palm oil or solid sludge grew in culture media with palm oil or solid sludge as the sole source of carbon and energy but with a difference in load. Thus, isolates BI2, BI5, BI31, BI10 and BI 9 showed the highest degradation capacities. These isolates could be used to constitute consortia of microorganisms that can be used in the treatment of waste generated by palm oil extraction. }, year = {2024} } </pre> <p class="cite_operation"> <span><a class="bib-copy-pop copy-el" data-clipboard-action="copy" data-clipboard-target=".bib-copy-pop-src" href="javascript:;"><img src="/img/copy_icon.png">Copy</a></span> <span class="line">|</span> <span><a href="javascript:;" onclick="spgCommon.bindDownloadDataFromEl("10.11648.j.fem.20241002.11.bib", '.bib-copy-pop-src')"><img src="/img/download_icon.png">Download</a></span> </p> </div> </li> <li class="mt-tabpage-item puple_cite"> <div class="tab_div"> <pre _ngcontent-anj-c150="" class="text ris-text ris-copy-pop-src">TY - JOUR T1 - Isolation and Characterization of Indigenous Bacteria with Purifying Potential in Solid Palm Oil Extraction Sludge Generated by SOCAPALM-Mbambou AU - Tassong Saah Denis AU - Simo Claude AU - Taffouo Victor Désiré Y1 - 2024/04/17 PY - 2024 N1 - https://doi.org/10.11648/j.fem.20241002.11 DO - 10.11648/j.fem.20241002.11 T2 - Frontiers in Environmental Microbiology JF - Frontiers in Environmental Microbiology JO - Frontiers in Environmental Microbiology SP - 29 EP - 44 PB - Science Publishing Group SN - 2469-8067 UR - https://doi.org/10.11648/j.fem.20241002.11 AB - The oil palm industry contributes significantly to the economic development of producing countries such as Cameroon. Unfortunately, the exploitation of palm oil constitutes a source of environmental pollution due to the production of enormous quantities of waste during its extraction process, including solid sludge generating greenhouse gases which contribute to global warming climatic. All this leads to the search for alternatives which consists of isolating and characterizing indigenous bacteria with biodegradation capacities in sludge from palm oil extraction. The pH and bacterial counts were determined by the potentiometric method and the decimal dilution technique, respectively. The isolated bacteria were identified by their cultural, cellular and biochemical characteristics. In addition, the identification of Gram- bacteria was further explored by the API 20 E gallery. The palm oil biodegradability test was carried out on M2 medium supplemented with 2% palm oil. The solid sludge biodegradability test was carried out on liquid MSM medium supplemented with 4% sludge stock solution. The results showed that the sludge sample had a slightly alkaline pH of 7.3. A bacterial load of around 109 CFU/g of soil was counted. Thirty-one bacterial strains were isolated and purified, including 12 Bacillus sp, 10 Pseudomonas sp, 8 Proteus mirabilis and 1 Klebsiella pneumoniae. All isolates tested for their ability to degrade palm oil or solid sludge grew in culture media with palm oil or solid sludge as the sole source of carbon and energy but with a difference in load. Thus, isolates BI2, BI5, BI31, BI10 and BI 9 showed the highest degradation capacities. These isolates could be used to constitute consortia of microorganisms that can be used in the treatment of waste generated by palm oil extraction. 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