TNF-alpha Products

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<div class="highlighted"> <aside class="container section clearfix" role="complementary"> <div> </div> </aside> </div> <div id="main" class="container-fluid container-wide "> <div class="row"> <div class="col-md-12"> <div> <div id="block-bio-techne-global-breadcrumbs" class="system_breadcrumb_block"> <nav role="navigation" aria-labelledby="system-breadcrumb"> <h2 id="system-breadcrumb" class="visually-hidden">Breadcrumb</h2> <ol> <li> <a href="/">Home</a> </li> <li> Products </li> <li> TNF-alpha Products </li> </ol> </nav> </div> </div> </div> </div> <div class="row"> <div class="col-md-12"> <main> <section class="section"> <div> <div id="block-bio-techne-global-content" class="system_main_block"> <div id="search_results_page"> <div class="d-sm-block d-xs-block d-lg-none d-xl-none"> <div class="d-lg-none"> <h1>TNF-alpha Products</h1> <div class="show-more" data-text="<p>TNF-alpha (Tumor necrosis factor alpha) plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism. TNF-alpha was first identified as a cytotoxic factor produced by macrophages capable of killing mouse tumor cells.&nbsp;It is the prototypic ligand and along with Lymphotoxin-alpha, were identified as the first members of the TNF superfamily. Active TNF-alpha and other members of the TNF superfamily exist as a homotrimer with high structural homology. Receptor binding occurs at the interface of two TNF-alpha monomers. And receptor activation occurs when all three monomer interfaces are engaged with a receptor. For TNF-alpha, receptor binding and activation occurs through TNF R1 or TNF RII, and subsequently leads to activation of NF-kB or MAPK signaling pathways. Another pathway that TNF-alpha can activate utilizes the death domain of TNF RI to induce apoptosis. TNF-alpha promotes the inflammatory response largely through NF-kB signaling, and inhibition of TNF-alpha has proven successful in treating many autoimmune disorders. TNF-alpha is also present on the cell surface as membrane-bound TNF-alpha can induce the lysis of neighboring tumor cells and virus infected cells. TNF-alpha protein is translated as a type II transmembrane protein containing an N-terminal transmembrane domain. The soluble cytokine is released from its cell-anchoring TM domain by proteolytic processing by metalloproteases.</p>"> <div class="data-display"> <p>TNF-alpha (Tumor necrosis factor alpha) plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism. TNF-alpha was first identified as a cytotoxic factor produced by macrophages capable of killing mouse tumor cells. It is the prototypic ligand and along with Lymphotoxin-alpha, were identified as the first members of the TNF superfamily. Active TNF-alpha and other members of the TNF superfamily exist as a homotrimer with high structural homology. Receptor binding occurs at the interface of two TNF-alpha monomers. And receptor activation occurs when all three monomer interfaces are engaged with a receptor. For TNF-alpha, receptor binding and activation occurs through TNF R1 or TNF RII, and subsequently leads to activation of NF-kB or MAPK signaling pathways. Another pathway that TNF-alpha can activate utilizes the death domain of TNF RI to induce apoptosis. TNF-alpha promotes the inflammatory response largely through NF-kB signaling, and inhibition of TNF-alpha has proven successful in treating many autoimmune disorders. TNF-alpha is also present on the cell surface as membrane-bound TNF-alpha can induce the lysis of neighboring tumor cells and virus infected cells. TNF-alpha protein is translated as a type II transmembrane protein containing an N-terminal transmembrane domain. 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a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="VersaClone cDNA Plasmids (4)" data-facet_lower="versaclone cdna plasmids" class="search_facet_name text-break sub_category"> VersaClone cDNA Plasmids <span class="facet_count">(4)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?sub_category=Luminex Discovery Assays" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Luminex Discovery Assays (3)" data-facet_lower="luminex discovery assays" class="search_facet_name text-break sub_category"> Luminex Discovery Assays <span class="facet_count">(3)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?sub_category=Animal-Free Recombinant Proteins" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> 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a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Chemiluminescent Sandwich ELISAs (1)" data-facet_lower="chemiluminescent sandwich elisas" class="search_facet_name text-break sub_category"> Chemiluminescent Sandwich ELISAs <span class="facet_count">(1)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?sub_category=ELISpot Development Modules" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="ELISpot Development Modules (1)" data-facet_lower="elispot development modules" class="search_facet_name text-break sub_category"> ELISpot Development Modules <span class="facet_count">(1)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?sub_category=Overexpression Lysates" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Overexpression Lysates (1)" data-facet_lower="overexpression lysates" class="search_facet_name text-break sub_category"> Overexpression Lysates <span class="facet_count">(1)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?sub_category=Simple Plex Custom Multi-Analyte Assay Cartridges" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Simple Plex Custom Multi-Analyte Assay Cartridges (1)" data-facet_lower="simple plex custom multi-analyte assay cartridges" class="search_facet_name text-break sub_category"> Simple Plex Custom Multi-Analyte Assay Cartridges <span class="facet_count">(1)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?sub_category=Single Analyte ELISpot Kits" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Single Analyte ELISpot Kits (1)" data-facet_lower="single analyte elispot kits" class="search_facet_name text-break sub_category"> Single Analyte ELISpot Kits <span class="facet_count">(1)</span> </span> </a> </div> </div> </div> </div> </div> <div class="search_facet_category_block"> <div class="dropdown-container position-relative"> <button class="btn btn-secondary dropdown-toggle horizontal-dropdown" type="button" id="dropdown-menu-button-compatible_instrument" data-toggle="dropdown" aria-haspopup="true" aria-expanded="false"> Compatible Instrument </button> <div class="dropdown-menu" aria-labelledby="dropdown-menu-button-compatible_instrument"> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?compatible_instrument=Ella" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Ella (11)" data-facet_lower="ella" class="search_facet_name text-break compatible_instrument"> Ella <span class="facet_count">(11)</span> </span> </a> </div> </div> </div> </div> <div class="search_facet_category_block"> <div class="dropdown-container position-relative"> <button class="btn btn-secondary dropdown-toggle horizontal-dropdown" type="button" id="dropdown-menu-button-species" data-toggle="dropdown" aria-haspopup="true" aria-expanded="false"> Species </button> <div class="dropdown-menu" aria-labelledby="dropdown-menu-button-species"> <div class="facet search_facet_search_category facet_list p-1"> <input type="text" class="form-control w-100 my-2 facet_category_search species" data-facet_title="species" placeholder="Search Species..."/> </div> <div class="facets_wrapper"> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?species=Human" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Human (838)" data-facet_lower="human" class="search_facet_name text-break species"> Human <span class="facet_count">(838)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?species=Mouse" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Mouse (207)" data-facet_lower="mouse" class="search_facet_name text-break species"> Mouse <span class="facet_count">(207)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?species=Rat" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Rat (108)" data-facet_lower="rat" class="search_facet_name text-break species"> Rat <span class="facet_count">(108)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?species=Fish" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Fish (55)" data-facet_lower="fish" class="search_facet_name text-break species"> Fish <span class="facet_count">(55)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?species=Canine" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Canine (42)" data-facet_lower="canine" class="search_facet_name text-break species"> Canine <span class="facet_count">(42)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?species=Porcine" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Porcine (12)" data-facet_lower="porcine" class="search_facet_name text-break species"> Porcine <span class="facet_count">(12)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?species=Non-human Primate" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Non-human Primate (3)" data-facet_lower="non-human primate" class="search_facet_name text-break species"> Non-human Primate <span class="facet_count">(3)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?species=Primate - Macaca mulatta (Rhesus Macaque)" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Primate - Macaca mulatta (Rhesus Macaque) (1)" data-facet_lower="primate - macaca mulatta (rhesus macaque)" class="search_facet_name text-break species"> Primate - Macaca mulatta (Rhesus Macaque) <span class="facet_count">(1)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?species=Rabbit" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Rabbit (36)" data-facet_lower="rabbit" class="search_facet_name text-break species"> Rabbit <span class="facet_count">(36)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?species=Mouse (Negative)" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Mouse (Negative) (29)" data-facet_lower="mouse (negative)" class="search_facet_name text-break species"> Mouse (Negative) <span class="facet_count">(29)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?species=Sheep" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Sheep (25)" data-facet_lower="sheep" class="search_facet_name text-break species"> Sheep <span class="facet_count">(25)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?species=Feline" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Feline (11)" data-facet_lower="feline" class="search_facet_name text-break species"> Feline <span class="facet_count">(11)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?species=Guinea Pig" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Guinea Pig (11)" data-facet_lower="guinea pig" class="search_facet_name text-break species"> Guinea Pig <span class="facet_count">(11)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?species=Zebrafish" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Zebrafish (9)" data-facet_lower="zebrafish" class="search_facet_name text-break species"> Zebrafish <span class="facet_count">(9)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?species=Rhesus Macaque" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Rhesus Macaque (8)" data-facet_lower="rhesus macaque" class="search_facet_name text-break species"> Rhesus Macaque <span class="facet_count">(8)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?species=Monkey" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Monkey (7)" data-facet_lower="monkey" class="search_facet_name text-break species"> Monkey <span class="facet_count">(7)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?species=Cotton Rat" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Cotton Rat (5)" data-facet_lower="cotton rat" class="search_facet_name text-break species"> Cotton Rat <span class="facet_count">(5)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?species=Primate" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Primate (5)" data-facet_lower="primate" class="search_facet_name text-break species"> Primate <span class="facet_count">(5)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?species=Bovine" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Bovine (4)" data-facet_lower="bovine" class="search_facet_name text-break species"> Bovine <span class="facet_count">(4)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?species=Equine" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Equine (3)" data-facet_lower="equine" class="search_facet_name text-break species"> Equine <span class="facet_count">(3)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?species=Cynomolgus Monkey" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Cynomolgus Monkey (2)" data-facet_lower="cynomolgus monkey" class="search_facet_name text-break species"> Cynomolgus Monkey <span class="facet_count">(2)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?species=Avian - Chicken" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Avian - Chicken (1)" data-facet_lower="avian - chicken" class="search_facet_name text-break species"> Avian - Chicken <span class="facet_count">(1)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?species=Chicken" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Chicken (1)" data-facet_lower="chicken" class="search_facet_name text-break species"> Chicken <span class="facet_count">(1)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?species=Ferret" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Ferret (1)" data-facet_lower="ferret" class="search_facet_name text-break species"> Ferret <span class="facet_count">(1)</span> </span> </a> </div> </div> </div> </div> </div> <div class="search_facet_category_block"> <div class="dropdown-container position-relative"> <button class="btn btn-secondary dropdown-toggle horizontal-dropdown" type="button" id="dropdown-menu-button-applications" data-toggle="dropdown" aria-haspopup="true" aria-expanded="false"> Application </button> <div class="dropdown-menu" aria-labelledby="dropdown-menu-button-applications"> <div class="facet search_facet_search_category facet_list p-1"> <input type="text" class="form-control w-100 my-2 facet_category_search applications" data-facet_title="applications" placeholder="Search Application..."/> </div> <div class="facets_wrapper"> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?applications=ELISA" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="ELISA (557)" data-facet_lower="elisa" class="search_facet_name text-break applications"> ELISA <span class="facet_count">(557)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?applications=Western Blot" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Western Blot (319)" data-facet_lower="western blot" class="search_facet_name text-break applications"> Western Blot <span class="facet_count">(319)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?applications=Immunocytochemistry/ Immunofluorescence" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Immunocytochemistry/ Immunofluorescence (137)" data-facet_lower="immunocytochemistry/ immunofluorescence" class="search_facet_name text-break applications"> Immunocytochemistry/ Immunofluorescence <span class="facet_count">(137)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?applications=Bioactivity" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Bioactivity (28)" data-facet_lower="bioactivity" class="search_facet_name text-break applications"> Bioactivity <span class="facet_count">(28)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?applications=Block/Neutralize" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Block/Neutralize (265)" data-facet_lower="block/neutralize" class="search_facet_name text-break applications"> Block/Neutralize <span class="facet_count">(265)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?applications=Simple Plex Automated Immunoassay" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Simple Plex Automated Immunoassay (8)" data-facet_lower="simple plex automated immunoassay" class="search_facet_name text-break applications"> Simple Plex Automated Immunoassay <span class="facet_count">(8)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?applications=Flow Cytometry" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Flow Cytometry (607)" data-facet_lower="flow cytometry" class="search_facet_name text-break applications"> Flow Cytometry <span class="facet_count">(607)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?applications=Immunohistochemistry" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Immunohistochemistry (264)" data-facet_lower="immunohistochemistry" class="search_facet_name text-break applications"> Immunohistochemistry <span class="facet_count">(264)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?applications=ELISA Capture" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="ELISA Capture (10)" data-facet_lower="elisa capture" class="search_facet_name text-break applications"> ELISA Capture <span class="facet_count">(10)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?applications=CyTOF-ready" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="CyTOF-ready (137)" data-facet_lower="cytof-ready" class="search_facet_name text-break applications"> CyTOF-ready <span class="facet_count">(137)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?applications=ELISA Detection" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="ELISA Detection (8)" data-facet_lower="elisa detection" class="search_facet_name text-break applications"> ELISA Detection <span class="facet_count">(8)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?applications=Control" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Control (6)" data-facet_lower="control" class="search_facet_name text-break applications"> Control <span class="facet_count">(6)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?applications=Functional Assay" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Functional Assay (318)" data-facet_lower="functional assay" class="search_facet_name text-break applications"> Functional Assay <span class="facet_count">(318)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?applications=SDS-PAGE" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="SDS-PAGE (9)" data-facet_lower="sds-page" class="search_facet_name text-break applications"> SDS-PAGE <span class="facet_count">(9)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?applications=Inhibition Activity" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Inhibition Activity (2)" data-facet_lower="inhibition activity" class="search_facet_name text-break applications"> Inhibition Activity <span class="facet_count">(2)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?applications=Antibody Competition" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Antibody Competition (1)" data-facet_lower="antibody competition" class="search_facet_name text-break applications"> Antibody Competition <span class="facet_count">(1)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?applications=Dual RNAscope ISH-IHC" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Dual RNAscope ISH-IHC (1)" data-facet_lower="dual rnascope ish-ihc" class="search_facet_name text-break applications"> Dual RNAscope ISH-IHC <span class="facet_count">(1)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?applications=Enzyme Activity" rel="nofollow" class="facet_item"> <span class="styled_checkbox a___button-checkbox"> <span class="checkbox_square"></span> <span class="checkbox_square_inner"></span> </span> <span data-facet="Enzyme Activity (1)" data-facet_lower="enzyme activity" class="search_facet_name text-break applications"> Enzyme Activity <span class="facet_count">(1)</span> </span> </a> </div> </div> </div> </div> </div> <div class="search_facet_category_block"> <div class="dropdown-container position-relative"> <button class="btn btn-secondary dropdown-toggle horizontal-dropdown" type="button" id="dropdown-menu-button-conjugate_form" data-toggle="dropdown" aria-haspopup="true" aria-expanded="false"> Conjugates </button> <div class="dropdown-menu" aria-labelledby="dropdown-menu-button-conjugate_form"> <div class="facet search_facet_search_category facet_list p-1"> <input type="text" class="form-control w-100 my-2 facet_category_search conjugate_form" data-facet_title="conjugate_form" placeholder="Search Conjugates..."/> </div> <div class="facets_wrapper"> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?conjugate_form=Unconjugated" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Unconjugated (185)" data-facet_lower="unconjugated" class="search_facet_name text-break conjugate_form"> Unconjugated <span class="facet_count">(185)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?conjugate_form=HRP" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="HRP (38)" data-facet_lower="hrp" class="search_facet_name text-break conjugate_form"> HRP <span class="facet_count">(38)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?conjugate_form=Biotin" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Biotin (53)" data-facet_lower="biotin" class="search_facet_name text-break conjugate_form"> Biotin <span class="facet_count">(53)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?conjugate_form=Alexa Fluor 350" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Alexa Fluor 350 (31)" data-facet_lower="alexa fluor 350" class="search_facet_name text-break conjugate_form"> Alexa Fluor 350 <span class="facet_count">(31)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?conjugate_form=Alexa Fluor 405" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Alexa Fluor 405 (31)" data-facet_lower="alexa fluor 405" class="search_facet_name text-break conjugate_form"> Alexa Fluor 405 <span class="facet_count">(31)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?conjugate_form=Alexa Fluor 488" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Alexa Fluor 488 (31)" data-facet_lower="alexa fluor 488" class="search_facet_name text-break conjugate_form"> Alexa Fluor 488 <span class="facet_count">(31)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?conjugate_form=Alexa Fluor 594" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Alexa Fluor 594 (31)" data-facet_lower="alexa fluor 594" class="search_facet_name text-break conjugate_form"> Alexa Fluor 594 <span class="facet_count">(31)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?conjugate_form=Alexa Fluor 647" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Alexa Fluor 647 (31)" data-facet_lower="alexa fluor 647" class="search_facet_name text-break conjugate_form"> Alexa Fluor 647 <span class="facet_count">(31)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?conjugate_form=Alexa Fluor 700" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Alexa Fluor 700 (31)" data-facet_lower="alexa fluor 700" class="search_facet_name text-break conjugate_form"> Alexa Fluor 700 <span class="facet_count">(31)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?conjugate_form=Alexa Fluor 750" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Alexa Fluor 750 (31)" data-facet_lower="alexa fluor 750" class="search_facet_name text-break conjugate_form"> Alexa Fluor 750 <span class="facet_count">(31)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?conjugate_form=FITC" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="FITC (31)" data-facet_lower="fitc" class="search_facet_name text-break conjugate_form"> FITC <span class="facet_count">(31)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?conjugate_form=Alexa Fluor 532" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Alexa Fluor 532 (28)" data-facet_lower="alexa fluor 532" class="search_facet_name text-break conjugate_form"> Alexa Fluor 532 <span class="facet_count">(28)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?conjugate_form=DyLight 350" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="DyLight 350 (28)" data-facet_lower="dylight 350" class="search_facet_name text-break conjugate_form"> DyLight 350 <span class="facet_count">(28)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?conjugate_form=DyLight 405" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="DyLight 405 (28)" data-facet_lower="dylight 405" class="search_facet_name text-break conjugate_form"> DyLight 405 <span class="facet_count">(28)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?conjugate_form=DyLight 488" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="DyLight 488 (28)" data-facet_lower="dylight 488" class="search_facet_name text-break conjugate_form"> DyLight 488 <span class="facet_count">(28)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?conjugate_form=DyLight 550" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="DyLight 550 (28)" data-facet_lower="dylight 550" class="search_facet_name text-break conjugate_form"> DyLight 550 <span class="facet_count">(28)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?conjugate_form=DyLight 594" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="DyLight 594 (28)" data-facet_lower="dylight 594" class="search_facet_name text-break conjugate_form"> DyLight 594 <span class="facet_count">(28)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?conjugate_form=DyLight 650" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="DyLight 650 (28)" data-facet_lower="dylight 650" class="search_facet_name text-break conjugate_form"> DyLight 650 <span class="facet_count">(28)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?conjugate_form=DyLight 680" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="DyLight 680 (28)" data-facet_lower="dylight 680" class="search_facet_name text-break conjugate_form"> DyLight 680 <span class="facet_count">(28)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?conjugate_form=DyLight 755" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="DyLight 755 (28)" data-facet_lower="dylight 755" class="search_facet_name text-break conjugate_form"> DyLight 755 <span class="facet_count">(28)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?conjugate_form=Janelia Fluor 549" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Janelia Fluor 549 (27)" data-facet_lower="janelia fluor 549" class="search_facet_name text-break conjugate_form"> Janelia Fluor 549 <span class="facet_count">(27)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?conjugate_form=Janelia Fluor 646" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Janelia Fluor 646 (27)" data-facet_lower="janelia fluor 646" class="search_facet_name text-break conjugate_form"> Janelia Fluor 646 <span class="facet_count">(27)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?conjugate_form=mFluor Violet 450 SE" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="mFluor Violet 450 SE (27)" data-facet_lower="mfluor violet 450 se" class="search_facet_name text-break conjugate_form"> mFluor Violet 450 SE <span class="facet_count">(27)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?conjugate_form=mFluor Violet 500 SE" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="mFluor Violet 500 SE (27)" data-facet_lower="mfluor violet 500 se" class="search_facet_name text-break conjugate_form"> mFluor Violet 500 SE <span class="facet_count">(27)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?conjugate_form=mFluor Violet 610 SE" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="mFluor Violet 610 SE (27)" data-facet_lower="mfluor violet 610 se" class="search_facet_name text-break conjugate_form"> mFluor Violet 610 SE <span class="facet_count">(27)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?conjugate_form=PerCP" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="PerCP (26)" data-facet_lower="percp" class="search_facet_name text-break conjugate_form"> PerCP <span class="facet_count">(26)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?conjugate_form=CoraFluor 1" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="CoraFluor 1 (25)" data-facet_lower="corafluor 1" class="search_facet_name text-break conjugate_form"> CoraFluor 1 <span class="facet_count">(25)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?conjugate_form=Allophycocyanin" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Allophycocyanin (23)" data-facet_lower="allophycocyanin" class="search_facet_name text-break conjugate_form"> Allophycocyanin <span class="facet_count">(23)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?conjugate_form=PE" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="PE (22)" data-facet_lower="pe" class="search_facet_name text-break conjugate_form"> PE <span class="facet_count">(22)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?conjugate_form=Fluorescein" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Fluorescein (3)" data-facet_lower="fluorescein" class="search_facet_name text-break conjugate_form"> Fluorescein <span class="facet_count">(3)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?conjugate_form=Phycoerythrin" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Phycoerythrin (3)" data-facet_lower="phycoerythrin" class="search_facet_name text-break conjugate_form"> Phycoerythrin <span class="facet_count">(3)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?conjugate_form=Allophycocyanin/Cy7" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Allophycocyanin/Cy7 (1)" data-facet_lower="allophycocyanin/cy7" class="search_facet_name text-break conjugate_form"> Allophycocyanin/Cy7 <span class="facet_count">(1)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?conjugate_form=PE/Atto594" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="PE/Atto594 (1)" data-facet_lower="pe/atto594" class="search_facet_name text-break conjugate_form"> PE/Atto594 <span class="facet_count">(1)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?conjugate_form=PE/Cy5.5" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="PE/Cy5.5 (1)" data-facet_lower="pe/cy5.5" class="search_facet_name text-break conjugate_form"> PE/Cy5.5 <span class="facet_count">(1)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?conjugate_form=PE/Cy7" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="PE/Cy7 (1)" data-facet_lower="pe/cy7" class="search_facet_name text-break conjugate_form"> PE/Cy7 <span class="facet_count">(1)</span> </span> </a> </div> </div> </div> </div> </div> <div class="search_facet_category_block"> <div class="dropdown-container position-relative"> <button class="btn btn-secondary dropdown-toggle more-facet" type="button" id="dropdown-menu-button-clonality" data-toggle="dropdown" aria-haspopup="true" aria-expanded="false"> Clonality </button> <div class="dropdown-menu" aria-labelledby="dropdown-menu-button-clonality"> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?clonality=Polyclonal" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Polyclonal (49)" data-facet_lower="polyclonal" class="search_facet_name text-break clonality"> Polyclonal <span class="facet_count">(49)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?clonality=Monoclonal" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Monoclonal (839)" data-facet_lower="monoclonal" class="search_facet_name text-break clonality"> Monoclonal <span class="facet_count">(839)</span> </span> </a> </div> </div> </div> </div> <div class="search_facet_category_block"> <div class="dropdown-container position-relative"> <button class="btn btn-secondary dropdown-toggle more-facet" type="button" id="dropdown-menu-button-host" data-toggle="dropdown" aria-haspopup="true" aria-expanded="false"> Host Species </button> <div class="dropdown-menu" aria-labelledby="dropdown-menu-button-host"> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?host=Mouse" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Mouse (270)" data-facet_lower="mouse" class="search_facet_name text-break host"> Mouse <span class="facet_count">(270)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?host=Goat" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Goat (20)" data-facet_lower="goat" class="search_facet_name text-break host"> Goat <span class="facet_count">(20)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?host=Rabbit" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Rabbit (167)" data-facet_lower="rabbit" class="search_facet_name text-break host"> Rabbit <span class="facet_count">(167)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?host=Human" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Human (392)" data-facet_lower="human" class="search_facet_name text-break host"> Human <span class="facet_count">(392)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?host=Rat" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Rat (41)" data-facet_lower="rat" class="search_facet_name text-break host"> Rat <span class="facet_count">(41)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?host=Hamster" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Hamster (1)" data-facet_lower="hamster" class="search_facet_name text-break host"> Hamster <span class="facet_count">(1)</span> </span> </a> </div> </div> </div> </div> <div class="search_facet_category_block"> <div class="dropdown-container position-relative"> <button class="btn btn-secondary dropdown-toggle more-facet" type="button" id="dropdown-menu-button-isotype" data-toggle="dropdown" aria-haspopup="true" aria-expanded="false"> Isotype </button> <div class="dropdown-menu" aria-labelledby="dropdown-menu-button-isotype"> <div class="facets_wrapper"> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?isotype=IgG" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="IgG (137)" data-facet_lower="igg" class="search_facet_name text-break isotype"> IgG <span class="facet_count">(137)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?isotype=IgG1" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="IgG1 (425)" data-facet_lower="igg1" class="search_facet_name text-break isotype"> IgG1 <span class="facet_count">(425)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?isotype=IgG1 kappa" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="IgG1 kappa (172)" data-facet_lower="igg1 kappa" class="search_facet_name text-break isotype"> IgG1 kappa <span class="facet_count">(172)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?isotype=IgG Kappa" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="IgG Kappa (79)" data-facet_lower="igg kappa" class="search_facet_name text-break isotype"> IgG Kappa <span class="facet_count">(79)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?isotype=IgG4" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="IgG4 (29)" data-facet_lower="igg4" class="search_facet_name text-break isotype"> IgG4 <span class="facet_count">(29)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?isotype=IgG4 kappa" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="IgG4 kappa (27)" data-facet_lower="igg4 kappa" class="search_facet_name text-break isotype"> IgG4 kappa <span class="facet_count">(27)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?isotype=IgM Kappa" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="IgM Kappa (12)" data-facet_lower="igm kappa" class="search_facet_name text-break isotype"> IgM Kappa <span class="facet_count">(12)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?isotype=IgG2b" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="IgG2b (4)" data-facet_lower="igg2b" class="search_facet_name text-break isotype"> IgG2b <span class="facet_count">(4)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?isotype=IgG2a" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="IgG2a (2)" data-facet_lower="igg2a" class="search_facet_name text-break isotype"> IgG2a <span class="facet_count">(2)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?isotype=Serum" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Serum (1)" data-facet_lower="serum" class="search_facet_name text-break isotype"> Serum <span class="facet_count">(1)</span> </span> </a> </div> </div> </div> </div> </div> <div class="search_facet_category_block"> <div class="dropdown-container position-relative"> <button class="btn btn-secondary dropdown-toggle more-facet" type="button" id="dropdown-menu-button-clone" data-toggle="dropdown" aria-haspopup="true" aria-expanded="false"> Clone </button> <div class="dropdown-menu" aria-labelledby="dropdown-menu-button-clone"> <div class="facet search_facet_search_category facet_list p-1"> <input type="text" class="form-control w-100 my-2 facet_category_search clone" data-facet_title="clone" placeholder="Search Clone..."/> </div> <div class="facets_wrapper"> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?clone=1825" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="1825 (1)" data-facet_lower="1825" class="search_facet_name text-break clone"> 1825 <span class="facet_count">(1)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?clone=28401" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="28401 (1)" data-facet_lower="28401" class="search_facet_name text-break clone"> 28401 <span class="facet_count">(1)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?clone=cA2 (Infliximab)" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="cA2 (Infliximab) (57)" data-facet_lower="ca2 (infliximab)" class="search_facet_name text-break clone"> cA2 (Infliximab) <span class="facet_count">(57)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?clone=D2E7 (Adalimumab)" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="D2E7 (Adalimumab) (54)" data-facet_lower="d2e7 (adalimumab)" class="search_facet_name text-break clone"> D2E7 (Adalimumab) <span class="facet_count">(54)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?clone=CDP 571 (Humicade)" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="CDP 571 (Humicade) (51)" data-facet_lower="cdp 571 (humicade)" class="search_facet_name text-break clone"> CDP 571 (Humicade) <span class="facet_count">(51)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?clone=Hu7" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Hu7 (37)" data-facet_lower="hu7" class="search_facet_name text-break clone"> Hu7 <span class="facet_count">(37)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?clone=6402" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="6402 (36)" data-facet_lower="6402" class="search_facet_name text-break clone"> 6402 <span class="facet_count">(36)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?clone=MP6-XT22" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="MP6-XT22 (36)" data-facet_lower="mp6-xt22" class="search_facet_name text-break clone"> MP6-XT22 <span class="facet_count">(36)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?clone=6401" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="6401 (33)" data-facet_lower="6401" class="search_facet_name text-break clone"> 6401 <span class="facet_count">(33)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?clone=2342C" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="2342C (30)" data-facet_lower="2342c" class="search_facet_name text-break clone"> 2342C <span class="facet_count">(30)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?clone=6N2E12" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="6N2E12 (29)" data-facet_lower="6n2e12" class="search_facet_name text-break clone"> 6N2E12 <span class="facet_count">(29)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?clone=B-F7" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="B-F7 (29)" data-facet_lower="b-f7" class="search_facet_name text-break clone"> B-F7 <span class="facet_count">(29)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?clone=CDP571" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="CDP571 (29)" data-facet_lower="cdp571" class="search_facet_name text-break clone"> CDP571 <span class="facet_count">(29)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?clone=CMAB008" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="CMAB008 (29)" data-facet_lower="cmab008" class="search_facet_name text-break clone"> CMAB008 <span class="facet_count">(29)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?clone=ESBA 105" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="ESBA 105 (29)" data-facet_lower="esba 105" class="search_facet_name text-break clone"> ESBA 105 <span class="facet_count">(29)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?clone=Epitomics patent anti-TNFa" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Epitomics patent anti-TNFa (29)" data-facet_lower="epitomics patent anti-tnfa" class="search_facet_name text-break clone"> Epitomics patent anti-TNFa <span class="facet_count">(29)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?clone=J2D10" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="J2D10 (29)" data-facet_lower="j2d10" class="search_facet_name text-break clone"> J2D10 <span class="facet_count">(29)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?clone=Z74P2C5*A12" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="Z74P2C5*A12 (29)" data-facet_lower="z74p2c5*a12" class="search_facet_name text-break clone"> Z74P2C5*A12 <span class="facet_count">(29)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?clone=adalimumab" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="adalimumab (29)" data-facet_lower="adalimumab" class="search_facet_name text-break clone"> adalimumab <span class="facet_count">(29)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?clone=certolizumAb" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="certolizumAb (29)" data-facet_lower="certolizumab" class="search_facet_name text-break clone"> certolizumAb <span class="facet_count">(29)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?clone=golimumab" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="golimumab (29)" data-facet_lower="golimumab" class="search_facet_name text-break clone"> golimumab <span class="facet_count">(29)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?clone=hMAK195" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="hMAK195 (29)" data-facet_lower="hmak195" class="search_facet_name text-break clone"> hMAK195 <span class="facet_count">(29)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?clone=infliximab" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="infliximab (29)" data-facet_lower="infliximab" class="search_facet_name text-break clone"> infliximab <span class="facet_count">(29)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?clone=6N1E7" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="6N1E7 (28)" data-facet_lower="6n1e7" class="search_facet_name text-break clone"> 6N1E7 <span class="facet_count">(28)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?clone=TNF/1500R" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="TNF/1500R (26)" data-facet_lower="tnf/1500r" class="search_facet_name text-break clone"> TNF/1500R <span class="facet_count">(26)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?clone=52B83" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="52B83 (23)" data-facet_lower="52b83" class="search_facet_name text-break clone"> 52B83 <span class="facet_count">(23)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?clone=2C8" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="2C8 (9)" data-facet_lower="2c8" class="search_facet_name text-break clone"> 2C8 <span class="facet_count">(9)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?clone=103302" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="103302 (2)" data-facet_lower="103302" class="search_facet_name text-break clone"> 103302 <span class="facet_count">(2)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?clone=4C6-H8" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="4C6-H8 (2)" data-facet_lower="4c6-h8" class="search_facet_name text-break clone"> 4C6-H8 <span class="facet_count">(2)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?clone=MAb1" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="MAb1 (2)" data-facet_lower="mab1" class="search_facet_name text-break clone"> MAb1 <span class="facet_count">(2)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?clone=P/T2" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="P/T2 (2)" data-facet_lower="p/t2" class="search_facet_name text-break clone"> P/T2 <span class="facet_count">(2)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?clone=TNF706" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="TNF706 (2)" data-facet_lower="tnf706" class="search_facet_name text-break clone"> TNF706 <span class="facet_count">(2)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?clone=TNF706 %2B P/T2" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="TNF706 + P/T2 (2)" data-facet_lower="tnf706 + p/t2" class="search_facet_name text-break clone"> TNF706 + P/T2 <span class="facet_count">(2)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?clone=103304" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="103304 (1)" data-facet_lower="103304" class="search_facet_name text-break clone"> 103304 <span class="facet_count">(1)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?clone=103314" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="103314 (1)" data-facet_lower="103314" class="search_facet_name text-break clone"> 103314 <span class="facet_count">(1)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?clone=159811" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="159811 (1)" data-facet_lower="159811" class="search_facet_name text-break clone"> 159811 <span class="facet_count">(1)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?clone=159813" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="159813 (1)" data-facet_lower="159813" class="search_facet_name text-break clone"> 159813 <span class="facet_count">(1)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?clone=182303" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="182303 (1)" data-facet_lower="182303" class="search_facet_name text-break clone"> 182303 <span class="facet_count">(1)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?clone=182309" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="182309 (1)" data-facet_lower="182309" class="search_facet_name text-break clone"> 182309 <span class="facet_count">(1)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?clone=182312" rel="nofollow" class="facet_item"> <span class="styled_radio a___button-radio"> <span class="radio_circle"></span> <span class="radio_circle_inner"></span> </span> <span data-facet="182312 (1)" data-facet_lower="182312" class="search_facet_name text-break clone"> 182312 <span class="facet_count">(1)</span> </span> </a> </div> <div class="m___radio-filter facet"> <a href="/t/tnf-alpha?clone=236812" rel="nofollow" class="facet_item"> <span 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class="search_term_text">TNF-alpha</span>" in <strong id="search_category">Products</strong> </p> </div> <div class="col-md-6 text-lg-right"> <label for="sort_by_select">Sort by:</label> <select id="sort_by_select" class="sort_by_select rounded-0 p-0" aria-label="Sort by"> <option value="/t/tnf-alpha?&sort=ranking,desc" selected="selected" >Best Match </option> <option value="/t/tnf-alpha?&sort=title,asc" >Products (A-Z) </option> <option value="/t/tnf-alpha?&sort=product_created_date,desc" >Newest </option> <option value="/t/tnf-alpha?&sort=stars,desc" >Rating </option> </select> </div> </div> <div id="search_results" class="row mt-3" data-search_term="TNF-alpha"> <div class="col-lg-12"> <div class="d-none d-lg-block"> <p class="target_h1">TNF-alpha Products</p> <div class="show-more" data-text="<p>TNF-alpha (Tumor necrosis factor alpha) plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism. TNF-alpha was first identified as a cytotoxic factor produced by macrophages capable of killing mouse tumor cells.&nbsp;It is the prototypic ligand and along with Lymphotoxin-alpha, were identified as the first members of the TNF superfamily. Active TNF-alpha and other members of the TNF superfamily exist as a homotrimer with high structural homology. Receptor binding occurs at the interface of two TNF-alpha monomers. And receptor activation occurs when all three monomer interfaces are engaged with a receptor. For TNF-alpha, receptor binding and activation occurs through TNF R1 or TNF RII, and subsequently leads to activation of NF-kB or MAPK signaling pathways. Another pathway that TNF-alpha can activate utilizes the death domain of TNF RI to induce apoptosis. TNF-alpha promotes the inflammatory response largely through NF-kB signaling, and inhibition of TNF-alpha has proven successful in treating many autoimmune disorders. TNF-alpha is also present on the cell surface as membrane-bound TNF-alpha can induce the lysis of neighboring tumor cells and virus infected cells. TNF-alpha protein is translated as a type II transmembrane protein containing an N-terminal transmembrane domain. The soluble cytokine is released from its cell-anchoring TM domain by proteolytic processing by metalloproteases.</p>"> <div class="data-display"> <p>TNF-alpha (Tumor necrosis factor alpha) plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism. TNF-alpha was first identified as a cytotoxic factor produced by macrophages capable of killing mouse tumor cells. It is the prototypic ligand and along with Lymphotoxin-alpha, were identified as the first members of the TNF superfamily. Active TNF-alpha and other members of the TNF superfamily exist as a homotrimer with high structural homology. Receptor binding occurs at the interface of two TNF-alpha monomers. And receptor activation occurs when all three monomer interfaces are engaged with a receptor. For TNF-alpha, receptor binding and activation occurs through TNF R1 or TNF RII, and subsequently leads to activation of NF-kB or MAPK signaling pathways. Another pathway that TNF-alpha can activate utilizes the death domain of TNF RI to induce apoptosis. TNF-alpha promotes the inflammatory response largely through NF-kB signaling, and inhibition of TNF-alpha has proven successful in treating many autoimmune disorders. TNF-alpha is also present on the cell surface as membrane-bound TNF-alpha can induce the lysis of neighboring tumor cells and virus infected cells. TNF-alpha protein is translated as a type II transmembrane protein containing an N-terminal transmembrane domain. The soluble cytokine is released from its cell-anchoring TM domain by proteolytic processing by metalloproteases.</p> </div> <a href="#" class="show-more-btn">Show More</a> </div> </div> <div id="search_results_selected_facets" class="my-2"> </div> <div id="search_results_block"> <div class="search_result atc-container pb-3" data-brand="rnd" data-category="ELISA Kits" data-id="MTA00B" data-name="Mouse TNF-alpha Quantikine ELISA Kit" data-position="0"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/elisa-kits/mouse-tnf-alpha-quantikine-elisa-kit_mta00b" title="Mouse TNF-alpha Quantikine ELISA Kit"> <h4>Mouse TNF-alpha Quantikine ELISA Kit <span class="rounded align-middle p-1 badge best-seller" title="Best Seller">Best Seller</span> </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="R&D Systems, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-rnd-systems.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/elisa-kits/mouse-tnf-alpha-quantikine-elisa-kit_mta00b" title="Mouse TNF-alpha Quantikine ELISA Kit"> MTA00B </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/elisa-kits/mouse-tnf-alpha-quantikine-elisa-kit_mta00b#reviews" class="searchImage_star5"> (22) </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/elisa-kits/mouse-tnf-alpha-quantikine-elisa-kit_mta00b#citations"><span class="icon-icon-product-citations"></span>Citations (1161)</a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/elisa-kits/mouse-tnf-alpha-quantikine-elisa-kit_mta00b#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> <div class="d-none d-md-inline my-1"> <a href="#" class="search_result_images"><span class="icon-icon-image-camera-plus"></span> Images (5)</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> ELISA </div> <div class="d-none d-md-block"> </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-0" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-0"> <div class="row"> <div class="search_result_description col-xs-12 col-md-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Sensitivity:</strong></td> <td class="pb-1 w-75 text-break"> 7.21 pg/mL </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> ELISA </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Assay Range:</strong></td> <td class="pb-1 w-75 text-break"> 10.9 - 700 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma) </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="mta00b"></div> </div> <div class="search_result_image_wrapper col-xs-12 col-md-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="rnd"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src=https://resources.bio-techne.com/images/products/elisa/TNFalpha_MTA00_ELISA_1036.jpg data-srcset=https://resources.bio-techne.com/images/products/elisa/TNFalpha_MTA00_ELISA_1036.jpg alt="Mouse TNF-alpha ELISA Cell Culture Supernate Standard Curve" title="Mouse TNF-alpha ELISA Cell Culture Supernate Standard Curve" data-caption="" data-badges="" data-application="N/A" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> <span class="image-count-text align-middle">(5)</span> </div> </div> </div> <i data-src="https://resources.bio-techne.com/images/products/elisa/TNFalpha_MTA00_ELISA_1037.jpg" data-alt="Mouse TNF-alpha ELISA Serum/Plasma Standard Curve" title="Mouse TNF-alpha ELISA Serum/Plasma Standard Curve" data-caption="" data-badges="" data-application="N/A" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/elisa/TNFalpha_MTA00_ELISA_1151.jpg" data-alt="Mouse TNF-alpha ELISA Linearity" title="Mouse TNF-alpha ELISA Linearity" data-caption="" data-badges="" data-application="N/A" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/mta00b_mouse-tnf-alpha-quantikine-elisa-kit-4420241542504.jpg" data-alt="Mouse TNF-alpha ELISA Kit - Quantikine" title="Mouse TNF-alpha ELISA Kit - Quantikine" data-caption="HFD-induced plasma saturated free fatty acid (FFA) and lactate trigger production of proinflammatory cytokines and VEGF in mouse chondrocytes.(a~d) Relative mRNA levels of IL-6 (a), TNF-alpha (b), IL-1 beta (c) and VEGF (d) in the mouse primary chondrocytes treated with full or fractionated (>3 KD or " data-badges="" data-application="N/A" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/mta00b_mouse-tnf-alpha-quantikine-elisa-kit-2282024172362.jpg" data-alt="Detection of Mouse Mouse TNF-alpha Quantikine ELISA Kit by ELISA" title="Detection of Mouse Mouse TNF-alpha Quantikine ELISA Kit by ELISA" data-caption="PYC suppressed LPS-induced proinflammatory cytokine production in BV2 microglia.Cells were incubated with the indicated concentrations of PYC or vehicle for 1h before LPS treatment (500ng/mL). After 24 h incubation, the culture supernatants were collected, and the amount of TNF-alpha, IL-6, IL-1 beta and IL-10 were measured by ELISA (A-D). Data are represented as mean ± SD from at least 3 independent experiments. *P< 0.05 compared with LPS alone. #P< 0.05. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26367267), licensed under a CC-BY license. Not internally tested by R&D Systems." data-badges="" data-application="ELISA" data-width="250" data-height="250" class="none"></i> </div> </div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-MTA00B" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="MTA00B"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart-MTA00B" class="atc-lines"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="MTA00B-1"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> MTA00B-1 </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="PMTA00B"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> PMTA00B </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="SMTA00B"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> SMTA00B </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> </div> <div class="atc-control ProductCart-MTA00B-control text-right"> <div class="row d-flex justify-content-end mx-0 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=MTA00B" class="atc-bulk-button btn a___button-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> </div> </div> <div class="search_result atc-container pb-3" data-brand="rnd" data-category="ELISA Kits" data-id="DTA00D" data-name="Human TNF-alpha Quantikine ELISA Kit" data-position="1"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/elisa-kits/human-tnf-alpha-quantikine-elisa-kit_dta00d" title="Human TNF-alpha Quantikine ELISA Kit"> <h4>Human TNF-alpha Quantikine ELISA Kit <span class="rounded align-middle p-1 badge best-seller" title="Best Seller">Best Seller</span> </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="R&D Systems, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-rnd-systems.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/elisa-kits/human-tnf-alpha-quantikine-elisa-kit_dta00d" title="Human TNF-alpha Quantikine ELISA Kit"> DTA00D </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/elisa-kits/human-tnf-alpha-quantikine-elisa-kit_dta00d#reviews" class="searchImage_star5"> (12) </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/elisa-kits/human-tnf-alpha-quantikine-elisa-kit_dta00d#citations"><span class="icon-icon-product-citations"></span>Citations (654)</a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/elisa-kits/human-tnf-alpha-quantikine-elisa-kit_dta00d#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> <div class="d-none d-md-inline my-1"> <a href="#" class="search_result_images"><span class="icon-icon-image-camera-plus"></span> Images (2)</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> ELISA </div> <div class="d-none d-md-block"> </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-1" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-1"> <div class="row"> <div class="search_result_description col-xs-12 col-md-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Sensitivity:</strong></td> <td class="pb-1 w-75 text-break"> 6.23 pg/mL </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> ELISA </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Assay Range:</strong></td> <td class="pb-1 w-75 text-break"> 15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma) </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="dta00d"></div> </div> <div class="search_result_image_wrapper col-xs-12 col-md-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="rnd"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src=https://resources.bio-techne.com/images/products/elisa/dta00d_human-tnf-alpha-quantikine-pharmpak-50-plates-2672021102137.jpg data-srcset=https://resources.bio-techne.com/images/products/elisa/dta00d_human-tnf-alpha-quantikine-pharmpak-50-plates-2672021102137.jpg alt="Human TNF-alpha ELISA Standard Curve" title="Human TNF-alpha ELISA Standard Curve" data-caption="" data-badges="" data-application="N/A" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> <span class="image-count-text align-middle">(2)</span> </div> </div> </div> <i data-src="https://resources.bio-techne.com/images/products/elisa/dta00d_human-tnf-alpha-quantikine-pharmpak-50-plates-2672021102236.jpg" data-alt="Human TNF-alpha ELISA Linearity" title="Human TNF-alpha ELISA Linearity" data-caption="" data-badges="" data-application="N/A" data-width="250" data-height="250" class="none"></i> </div> </div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-DTA00D" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="DTA00D"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart-DTA00D" class="atc-lines"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="DTA00D"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> DTA00D </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="PDTA00D"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> PDTA00D </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="STA00D"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> STA00D </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> </div> <div class="atc-control ProductCart-DTA00D-control text-right"> <div class="row d-flex justify-content-end mx-0 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=DTA00D" class="atc-bulk-button btn a___button-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> </div> </div> <div class="search_result atc-container pb-3" data-brand="proteinsimple" data-category="Simple Plex" data-id="SPCKB-MP-000822" data-name="Simple Plex Mouse TNF-alpha Cartridge" data-position="2"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/simple-plex/simple-plex-mouse-tnf-alpha-cartridge_spckb-mp-000822" title="Simple Plex Mouse TNF-alpha Cartridge"> <h4>Simple Plex Mouse TNF-alpha Cartridge </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="ProteinSimple, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-proteinsimple.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/simple-plex/simple-plex-mouse-tnf-alpha-cartridge_spckb-mp-000822" title="Simple Plex Mouse TNF-alpha Cartridge"> SPCKB-MP-000822 </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/simple-plex/simple-plex-mouse-tnf-alpha-cartridge_spckb-mp-000822#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> <div class="d-none d-md-inline my-1"> <a href="#" class="search_result_images"><span class="icon-icon-image-camera-plus"></span> Images (3)</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> Simple Plex </div> <div class="d-none d-md-block"> </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-2" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="row"> <div class="align-middle mt-2 col-xs-12 col-sm-9"> <p class="mb-0">Simple Plex Mouse TNF-alpha assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.</p> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-2"> <div class="row"> <div class="search_result_description col-xs-12 col-md-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> Simple Plex </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="spckb-mp-000822"></div> </div> <div class="search_result_image_wrapper col-xs-12 col-md-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="proteinsimple"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src=https://resources.bio-techne.com/images/products/spckb-mp-000822_simple-plex-cartridge-kit-for-16-samples-1-analyte-for-use-with-mouse-plasma-serum-279202113247.png data-srcset=https://resources.bio-techne.com/images/products/spckb-mp-000822_simple-plex-cartridge-kit-for-16-samples-1-analyte-for-use-with-mouse-plasma-serum-279202113247.png alt="Simple Plex Cartridge Kit for 16 samples, 1 analyte for use with Mouse Plasma / Serum." title="Mouse TNF-alpha Ella Assay" data-caption="" data-badges="" data-application="N/A" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> <span class="image-count-text align-middle">(3)</span> </div> </div> </div> <i data-src="https://resources.bio-techne.com/images/products/spckb-mp-000822_simple-plex-cartridge-kit-for-16-samples-1-analyte-for-use-with-mouse-plasma-serum-22920218413.png" data-alt="Mouse TNF-alpha Simple Assay Standard Curve" title="Mouse TNF-alpha Ella Assay Standard Curve" data-caption="" data-badges="" data-application="N/A" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/spckb-mp-000822_simple-plex-cartridge-kit-for-16-samples-1-analyte-for-use-with-mouse-plasma-serum-229202184452.jpg" data-alt="Mouse TNF-alpha Simple Assay Linearity" title="Mouse TNF-alpha Ella Assay Linearity" data-caption="" data-badges="" data-application="N/A" data-width="250" data-height="250" class="none"></i> </div> </div> </div> </div> <div class="js-form-item form-item pb-3 js-form-type-search form-item- js-form-item-"> <label>Customize Your Simple Plex Cartridge</label> <div id="order_detail_wrapper" class="order_detail_wrapper"> <h4></h4> <p>Create your custom Simple Plex cartridge for up to 8 targets. Choose from more than 250 targets across multiple species.</p> <hr class="a___border-dashed my-4"> <div class="text-right mb-2"> <a class="btn btn-primary text-white" href="/resources/simple-plex-panel-builder"> Build My Cartridge </a> </div> </div> </div> </div> </div> </div> <div class="search_result atc-container pb-3" data-brand="rnd" data-category="ELISA Kits" data-id="DY410" data-name="Mouse TNF-alpha DuoSet ELISA" data-position="3"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/elisa-kits/mouse-tnf-alpha-duoset-elisa_dy410" title="Mouse TNF-alpha DuoSet ELISA"> <h4>Mouse TNF-alpha DuoSet ELISA <span class="rounded align-middle p-1 badge best-seller" title="Best Seller">Best Seller</span> </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="R&D Systems, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-rnd-systems.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/elisa-kits/mouse-tnf-alpha-duoset-elisa_dy410" title="Mouse TNF-alpha DuoSet ELISA"> DY410 </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/elisa-kits/mouse-tnf-alpha-duoset-elisa_dy410#reviews" class="searchImage_star5"> (31) </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/elisa-kits/mouse-tnf-alpha-duoset-elisa_dy410#citations"><span class="icon-icon-product-citations"></span>Citations (1135)</a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/elisa-kits/mouse-tnf-alpha-duoset-elisa_dy410#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> <div class="d-none d-md-inline my-1"> <a href="#" class="search_result_images"><span class="icon-icon-image-camera-plus"></span> Images (1)</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> ELISA </div> <div class="d-none d-md-block"> </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-3" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-3"> <div class="row"> <div class="search_result_description col-xs-12 col-md-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Assay Range:</strong></td> <td class="pb-1 w-75 text-break"> 31.2 - 2,000 pg/mL </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> ELISA </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="dy410"></div> </div> <div class="search_result_image_wrapper col-xs-12 col-md-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="rnd"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src=https://resources.bio-techne.com/images/products/elisa/DATA_TNFalpha_DY410_ELISA_2323.jpg data-srcset=https://resources.bio-techne.com/images/products/elisa/DATA_TNFalpha_DY410_ELISA_2323.jpg alt="Mouse TNF-alpha ELISA Standard Curve" title="Mouse TNF-alpha ELISA Standard Curve" data-caption="" data-badges="" data-application="N/A" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-DY410" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="DY410"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart-DY410" class="atc-lines"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="DY410"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> DY410 </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="DY410-05"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> DY410-05 </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> </div> <div class="atc-control ProductCart-DY410-control text-right"> <div class="row d-flex justify-content-end mx-0 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=DY410" class="atc-bulk-button btn a___button-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> </div> </div> <div class="search_result atc-container pb-3" data-brand="rnd" data-category="ELISA Kits" data-id="QK210" data-name="Human TNF-alpha Quantikine QuicKit ELISA" data-position="4"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/elisa-kits/human-tnf-alpha-quantikine-quickit-elisa_qk210" title="Human TNF-alpha Quantikine QuicKit ELISA"> <h4>Human TNF-alpha Quantikine QuicKit ELISA <span class="rounded align-middle p-1 badge best-seller" title="Best Seller">Best Seller</span> </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="R&D Systems, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-rnd-systems.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/elisa-kits/human-tnf-alpha-quantikine-quickit-elisa_qk210" title="Human TNF-alpha Quantikine QuicKit ELISA"> QK210 </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/elisa-kits/human-tnf-alpha-quantikine-quickit-elisa_qk210#citations"><span class="icon-icon-product-citations"></span>Citations (5)</a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/elisa-kits/human-tnf-alpha-quantikine-quickit-elisa_qk210#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> <div class="d-none d-md-inline my-1"> <a href="#" class="search_result_images"><span class="icon-icon-image-camera-plus"></span> Images (5)</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> ELISA </div> <div class="d-none d-md-block"> </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-4" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-4"> <div class="row"> <div class="search_result_description col-xs-12 col-md-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Sensitivity:</strong></td> <td class="pb-1 w-75 text-break"> 7.02 pg/mL </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> ELISA </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Assay Range:</strong></td> <td class="pb-1 w-75 text-break"> 31.3 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma) </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="qk210"></div> </div> <div class="search_result_image_wrapper col-xs-12 col-md-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="rnd"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src=https://resources.bio-techne.com/images/products/elisa/TNFalpha_DTA00_ELISA_2688.jpg data-srcset=https://resources.bio-techne.com/images/products/elisa/TNFalpha_DTA00_ELISA_2688.jpg alt="Human TNF- alpha Standard Curve" title="Human TNF- alpha Standard Curve" data-caption="" data-badges="" data-application="N/A" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> <span class="image-count-text align-middle">(5)</span> </div> </div> </div> <i data-src="https://resources.bio-techne.com/images/products/elisa/TNFalpha_DTA00_ELISA_2686.jpg" data-alt="Human TNF- alpha Standard Curve" title="Human TNF- alpha Standard Curve" data-caption="" data-badges="" data-application="N/A" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/elisa/TNFalpha_QK210_ELISA_2703.jpg" data-alt="Human TNF-alpha ELISA Standard Curve" title="Human TNF-alpha ELISA Standard Curve" data-caption="" data-badges="" data-application="N/A" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/elisa/qk210_human-tnf-alpha-quantikine-quickit-elisa-elisa-292020151237.png" data-alt="" title="Human TNF-alpha QuicKit Spiked Recovery Competitor Comparison" data-caption="TNF-alpha is spiked at three known concentrations throughout the range of the assay and run to measure response of the spiked sample matrix. Serum recovery is 100% compared to 66% for the top competitor. EDTA plasma recovery is 102% compared to 59% for the top competitor. Heparin plasma recovery is 112% compared to 74% for the top competitor. In spike and recovery experiments, natural samples are spiked with the recombinant target analyte of interest to identify interference caused by sample matrices." data-badges="" data-application="ELISA" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/elisa/qk210_human-tnf-alpha-quantikine-quickit-elisa-elisa-292020151746.png" data-alt="" title="Human TNF-alpha QuicKit Spiked Linearity Competitor Comparison" data-caption="TNF-alpha is spiked at high concentration in various matrices and diluted with appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. The linearity is between 87%-105% compared to 122%-189% for the top competitor." data-badges="" data-application="ELISA" data-width="250" data-height="250" class="none"></i> </div> </div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-QK210" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="QK210"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart-QK210" class="atc-lines"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="QK210"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> QK210 </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> </div> <div class="atc-control ProductCart-QK210-control text-right"> <div class="row d-flex justify-content-end mx-0 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=QK210" class="atc-bulk-button btn a___button-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> </div> </div> <div class="search_result atc-container pb-3" data-brand="rnd" data-category="ELISA Kits" data-id="HSTA00E" data-name="Human TNF-alpha Quantikine HS ELISA" data-position="5"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/elisa-kits/human-tnf-alpha-quantikine-hs-elisa_hsta00e" title="Human TNF-alpha Quantikine HS ELISA"> <h4>Human TNF-alpha Quantikine HS ELISA <span class="rounded align-middle p-1 badge best-seller" title="Best Seller">Best Seller</span> </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="R&D Systems, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-rnd-systems.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/elisa-kits/human-tnf-alpha-quantikine-hs-elisa_hsta00e" title="Human TNF-alpha Quantikine HS ELISA"> HSTA00E </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/elisa-kits/human-tnf-alpha-quantikine-hs-elisa_hsta00e#reviews" class="searchImage_star5"> (9) </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/elisa-kits/human-tnf-alpha-quantikine-hs-elisa_hsta00e#citations"><span class="icon-icon-product-citations"></span>Citations (308)</a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/elisa-kits/human-tnf-alpha-quantikine-hs-elisa_hsta00e#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> <div class="d-none d-md-inline my-1"> <a href="#" class="search_result_images"><span class="icon-icon-image-camera-plus"></span> Images (8)</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> ELISA </div> <div class="d-none d-md-block"> </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-5" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-5"> <div class="row"> <div class="search_result_description col-xs-12 col-md-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Sensitivity:</strong></td> <td class="pb-1 w-75 text-break"> 0.049 pg/mL </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> ELISA </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Assay Range:</strong></td> <td class="pb-1 w-75 text-break"> 0.156 - 10 pg/mL (Serum, EDTA Plasma, Heparin Plasma) </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="hsta00e"></div> </div> <div class="search_result_image_wrapper col-xs-12 col-md-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="rnd"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src=https://resources.bio-techne.com/images/products/elisa/TNFalpha_HSTA0_ELISA_2666.jpg data-srcset=https://resources.bio-techne.com/images/products/elisa/TNFalpha_HSTA0_ELISA_2666.jpg alt="Human TNF-alpha Quantikine HS ELISA Kit Sensitivity Competitor Comparison" title="Human TNF-alpha Quantikine HS ELISA Kit Sensitivity Competitor Comparison" data-caption="" data-badges="" data-application="N/A" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> <span class="image-count-text align-middle">(8)</span> </div> </div> </div> <i data-src="https://resources.bio-techne.com/images/products/elisa/TNFalpha_HSTA00D_ELISA_1185.jpg" data-alt="Human TNF-alpha Standard Curve" title="Human TNF-alpha Standard Curve" data-caption="" data-badges="" data-application="N/A" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/elisa/DATA_TNFalpha_HSTA0_ELISA_898.jpg" data-alt="Human TNF-alpha High Sensitivity Standard Curve" title="Human TNF-alpha High Sensitivity Standard Curve" data-caption="" data-badges="" data-application="N/A" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/elisa/TNF-alpha_HSTA0_ELISA_404.jpg" data-alt="Human TNF-alpha High Sensitivity ELISA Linearity" title="Human TNF-alpha High Sensitivity ELISA Linearity" data-caption="" data-badges="" data-application="N/A" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/elisa/TNFalpha_HSTA0_ELISA_2667.jpg" data-alt="Human TNF-alpha Quantikine HS ELISA Kit Recovery Competitor Comparison" title="Human TNF-alpha Quantikine HS ELISA Kit Recovery Competitor Comparison" data-caption="" data-badges="" data-application="N/A" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/elisa/TNFalpha_HSTA00D_ELISA_1186.jpg" data-alt="Human TNF-alpha ELISA Linearity" title="Human TNF-alpha ELISA Linearity" data-caption="" data-badges="" data-application="N/A" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/elisa/TNFalpha_HSTA0_ELISA_1171.jpg" data-alt="Human TNF-alpha ELISA Standard Curve" title="Human TNF-alpha ELISA Standard Curve" data-caption="" data-badges="" data-application="N/A" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/elisa/TNFalpha_HSTA0_ELISA_1172.jpg" data-alt="Human TNF-alpha ELISA Linearity" title="Human TNF-alpha ELISA Linearity" data-caption="" data-badges="" data-application="N/A" data-width="250" data-height="250" class="none"></i> </div> </div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-HSTA00E" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="HSTA00E"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart-HSTA00E" class="atc-lines"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="HSTA00E"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> HSTA00E </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="PHSTA00E"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> PHSTA00E </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="SSTA00E"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> SSTA00E </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> </div> <div class="atc-control ProductCart-HSTA00E-control text-right"> <div class="row d-flex justify-content-end mx-0 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=HSTA00E" class="atc-bulk-button btn a___button-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> </div> </div> <div class="search_result atc-container pb-3" data-brand="rnd" data-category="Proteins and Enzymes" data-id="410-MT" data-name="Recombinant Mouse TNF-alpha (aa 80-235) Protein" data-position="6"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/proteins-enzymes/recombinant-mouse-tnf-alpha-aa-80-235-protein_410-mt" title="Recombinant Mouse TNF-alpha (aa 80-235) Protein"> <h4>Recombinant Mouse TNF-alpha (aa 80-235) Protein <span class="rounded align-middle p-1 badge best-seller" title="Best Seller">Best Seller</span> </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="R&D Systems, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-rnd-systems.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/proteins-enzymes/recombinant-mouse-tnf-alpha-aa-80-235-protein_410-mt" title="Recombinant Mouse TNF-alpha (aa 80-235) Protein"> 410-MT </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/proteins-enzymes/recombinant-mouse-tnf-alpha-aa-80-235-protein_410-mt#reviews" class="searchImage_star5"> (14) </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/proteins-enzymes/recombinant-mouse-tnf-alpha-aa-80-235-protein_410-mt#citations"><span class="icon-icon-product-citations"></span>Citations (518)</a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/proteins-enzymes/recombinant-mouse-tnf-alpha-aa-80-235-protein_410-mt#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> <div class="d-none d-md-inline my-1"> <a href="#" class="search_result_images"><span class="icon-icon-image-camera-plus"></span> Images (2)</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> BA </div> <div class="d-none d-md-block"> </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-6" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="row"> <div class="align-middle mt-2 col-xs-12 col-sm-9"> <p class="mb-0">Analyzed by SEC-MALS</p> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-6"> <div class="row"> <div class="search_result_description col-xs-12 col-md-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Source:</strong></td> <td class="pb-1 w-75 text-break"> E. coli </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Accession #:</strong></td> <td class="pb-1 w-75 text-break"> P06804 </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> BA </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="410-mt"></div> </div> <div class="search_result_image_wrapper col-xs-12 col-md-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="rnd"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src=https://resources.bio-techne.com/images/products/410-mt_recombinant-mouse-tnf-alpha-aa-80-235-protein-sec-mals-203202493319..jpg data-srcset=https://resources.bio-techne.com/images/products/410-mt_recombinant-mouse-tnf-alpha-aa-80-235-protein-sec-mals-203202493319..jpg alt="" title="Recombinant Mouse TNF‑ alpha aa 80‑235 Protein SEC-MALS." data-caption="Recombinant Mouse TNF‑ alpha Protein (Catalog # 410-MT) has a molecular weight (MW) of 50.8 kDa as analyzed by SEC-MALS, suggesting that this protein is a homotrimer." data-badges="" data-application="SEC-MALS" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> <span class="image-count-text align-middle">(2)</span> </div> </div> </div> <i data-src="https://resources.bio-techne.com/images/products/protein/410-mt_recombinant-mouse-tnf-alpha-aa-80-235-protein-bioactivity-1612202094353.jpg" data-alt="Graph showing dose-dependent bioactivity of mouse TNF-alpha protein" title="Bioactivity of Mouse TNF-alpha" data-caption="Recombinant mouse TNF-alpha (410-MT) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED<sub>50</sub> for this effect is 8-50 pg/mL." data-badges="" data-application="Bioactivity" data-width="250" data-height="250" class="none"></i> </div> </div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-410-MT" data-cf="1" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="410-MT"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div class="row"> <hr/> </div> <div class="col-12 py-2 font-weight-bold px-0"> Carrier Free <a href="#" data-toggle="tooltip" title="No added BSA"> <span class="icon-information-outline2 ml-1"></span> </a> </div> <div id="ProductCart-410-MT-cf" class="atc-lines type-cf"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="410-MT-100/CF"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> 410-MT-100/CF </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="410-MT-050/CF"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> 410-MT-050/CF </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="410-MT-025/CF"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> 410-MT-025/CF </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="410-MT-01M/CF"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> 410-MT-01M/CF </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="410-MT-010/CF"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> 410-MT-010/CF </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> </div> <div class="row"> <hr/> </div> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="col-12 py-2 font-weight-bold px-0"> With Carrier <a href="#" data-toggle="tooltip" title="Formulated with BSA to improve stability"> <span class="icon-information-outline2 ml-1"></span> </a> </div> <div id="ProductCart-410-MT-ca" class="atc-lines type-ca"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="410-MT-010"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> 410-MT-010 </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="410-MT-025"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> 410-MT-025 </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="410-MT-050"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> 410-MT-050 </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="410-MT-100"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> 410-MT-100 </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> </div> <div class="atc-control ProductCart-410-MT-control text-right"> <div class="row d-flex justify-content-end mx-0 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=410-MT" class="atc-bulk-button btn a___button-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> </div> </div> <div class="search_result atc-container pb-3" data-brand="proteinsimple" data-category="Simple Plex" data-id="SPCKB-PS-002803" data-name="Simple Plex Human TNF-alpha 2nd Gen Cartridge" data-position="7"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/simple-plex/simple-plex-human-tnf-alpha-2nd-gen-cartridge_spckb-ps-002803" title="Simple Plex Human TNF-alpha 2nd Gen Cartridge"> <h4>Simple Plex Human TNF-alpha 2nd Gen Cartridge </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="ProteinSimple, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-proteinsimple.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/simple-plex/simple-plex-human-tnf-alpha-2nd-gen-cartridge_spckb-ps-002803" title="Simple Plex Human TNF-alpha 2nd Gen Cartridge"> SPCKB-PS-002803 </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/simple-plex/simple-plex-human-tnf-alpha-2nd-gen-cartridge_spckb-ps-002803#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> <div class="d-none d-md-inline my-1"> <a href="#" class="search_result_images"><span class="icon-icon-image-camera-plus"></span> Images (3)</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> Simple Plex </div> <div class="d-none d-md-block"> </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-7" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="row"> <div class="align-middle mt-2 col-xs-12 col-sm-9"> <p class="mb-0">Simple Plex Human TNF-alpha 2nd Gen assay kit contains cartridge, sample diluent SD13, and wash buffer. Part # starting with ST01 ships in 2-3 days. Sample type indicated with PS (Plasma/Serum).</p> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-7"> <div class="row"> <div class="search_result_description col-xs-12 col-md-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> Simple Plex </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="spckb-ps-002803"></div> </div> <div class="search_result_image_wrapper col-xs-12 col-md-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="proteinsimple"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src=https://resources.rndsystems.com/categoryimages/proteinsimple_simple-plex-single-analyte-assay-cartridges.png data-srcset=https://resources.rndsystems.com/categoryimages/proteinsimple_simple-plex-single-analyte-assay-cartridges.png alt="Human TNF-alpha Assay - Simple Plex" title="Human TNF-alpha Assay - Simple Plex" data-caption="" data-badges="" data-application="N/A" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> <span class="image-count-text align-middle">(3)</span> </div> </div> </div> <i data-src="https://resources.bio-techne.com/images/products/TNFalpha_SPCKBPS002803_ELISA_2761.png" data-alt="Human TNF-alpha Assay Standard Curve" title="Human TNF-alpha Assay Standard Curve" data-caption="" data-badges="" data-application="N/A" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/TNFalpha_SPCKBPS002803_ELISA_2762.jpg" data-alt="Human TNF-alpha Assay Linearity" title="Human TNF-alpha Assay Linearity" data-caption="" data-badges="" data-application="N/A" data-width="250" data-height="250" class="none"></i> </div> </div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-SPCKB-PS-002803" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="SPCKB-PS-002803"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart-SPCKB-PS-002803" class="atc-lines"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="SPCKA-PS-002803"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> SPCKA-PS-002803 </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="SPCKB-PS-002803"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> SPCKB-PS-002803 </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="SPCKC-PS-002803"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> SPCKC-PS-002803 </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="ST01B-PS-002803"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> ST01B-PS-002803 </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> </div> <div class="atc-control ProductCart-SPCKB-PS-002803-control text-right"> <div class="row d-flex justify-content-end mx-0 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=SPCKB-PS-002803" class="atc-bulk-button btn a___button-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> </div> </div> <div class="search_result atc-container pb-3" data-brand="rnd" data-category="ELISA Kits" data-id="RTA00" data-name="Rat TNF-alpha Quantikine ELISA Kit" data-position="8"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/elisa-kits/rat-tnf-alpha-quantikine-elisa-kit_rta00" title="Rat TNF-alpha Quantikine ELISA Kit"> <h4>Rat TNF-alpha Quantikine ELISA Kit </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="R&D Systems, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-rnd-systems.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/elisa-kits/rat-tnf-alpha-quantikine-elisa-kit_rta00" title="Rat TNF-alpha Quantikine ELISA Kit"> RTA00 </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/elisa-kits/rat-tnf-alpha-quantikine-elisa-kit_rta00#reviews" class="searchImage_star5"> (13) </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/elisa-kits/rat-tnf-alpha-quantikine-elisa-kit_rta00#citations"><span class="icon-icon-product-citations"></span>Citations (508)</a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/elisa-kits/rat-tnf-alpha-quantikine-elisa-kit_rta00#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> <div class="d-none d-md-inline my-1"> <a href="#" class="search_result_images"><span class="icon-icon-image-camera-plus"></span> Images (2)</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> ELISA </div> <div class="d-none d-md-block"> </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-8" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-8"> <div class="row"> <div class="search_result_description col-xs-12 col-md-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Sensitivity:</strong></td> <td class="pb-1 w-75 text-break"> 5 pg/mL </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> ELISA </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Assay Range:</strong></td> <td class="pb-1 w-75 text-break"> 12.5 - 800 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma) </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="rta00"></div> </div> <div class="search_result_image_wrapper col-xs-12 col-md-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="rnd"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src=https://resources.bio-techne.com/images/products/elisa/DATA_TNFalpha_RTA00_ELISA_1099.jpg data-srcset=https://resources.bio-techne.com/images/products/elisa/DATA_TNFalpha_RTA00_ELISA_1099.jpg alt="Rat TNF-alpha ELISA Standard Curve" title="Rat TNF-alpha ELISA Standard Curve" data-caption="" data-badges="" data-application="N/A" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> <span class="image-count-text align-middle">(2)</span> </div> </div> </div> <i data-src="https://resources.bio-techne.com/images/products/elisa/TNF-alpha_PRTA00_ELISA_365.jpg" data-alt="Rat TNF-alpha ELISA Linearity" title="Rat TNF-alpha ELISA Linearity" data-caption="" data-badges="" data-application="N/A" data-width="250" data-height="250" class="none"></i> </div> </div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-RTA00" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="RTA00"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart-RTA00" class="atc-lines"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="PRTA00"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> PRTA00 </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="RTA00-1"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> RTA00-1 </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="SRTA00"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> SRTA00 </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> </div> <div class="atc-control ProductCart-RTA00-control text-right"> <div class="row d-flex justify-content-end mx-0 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=RTA00" class="atc-bulk-button btn a___button-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> </div> </div> <div class="search_result atc-container pb-3" data-brand="rnd" data-category="ELISA Kits" data-id="DY210" data-name="Human TNF-alpha DuoSet ELISA" data-position="9"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/elisa-kits/human-tnf-alpha-duoset-elisa_dy210" title="Human TNF-alpha DuoSet ELISA"> <h4>Human TNF-alpha DuoSet ELISA <span class="rounded align-middle p-1 badge best-seller" title="Best Seller">Best Seller</span> </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="R&D Systems, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-rnd-systems.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/elisa-kits/human-tnf-alpha-duoset-elisa_dy210" title="Human TNF-alpha DuoSet ELISA"> DY210 </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/elisa-kits/human-tnf-alpha-duoset-elisa_dy210#reviews" class="searchImage_star5"> (28) </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/elisa-kits/human-tnf-alpha-duoset-elisa_dy210#citations"><span class="icon-icon-product-citations"></span>Citations (825)</a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/elisa-kits/human-tnf-alpha-duoset-elisa_dy210#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> <div class="d-none d-md-inline my-1"> <a href="#" class="search_result_images"><span class="icon-icon-image-camera-plus"></span> Images (1)</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> ELISA </div> <div class="d-none d-md-block"> </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-9" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-9"> <div class="row"> <div class="search_result_description col-xs-12 col-md-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Assay Range:</strong></td> <td class="pb-1 w-75 text-break"> 15.6 - 1,000 pg/mL </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> ELISA </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="dy210"></div> </div> <div class="search_result_image_wrapper col-xs-12 col-md-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="rnd"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src=https://resources.bio-techne.com/images/products/elisa/DATA_TNFalpha_DY210_ELISA_1766.jpg data-srcset=https://resources.bio-techne.com/images/products/elisa/DATA_TNFalpha_DY210_ELISA_1766.jpg alt="Human TNF-alpha ELISA Standard Curve" title="Human TNF-alpha ELISA Standard Curve" data-caption="" data-badges="" data-application="N/A" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-DY210" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="DY210"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart-DY210" class="atc-lines"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="DY210"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> DY210 </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="DY210-05"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> DY210-05 </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> </div> <div class="atc-control ProductCart-DY210-control text-right"> <div class="row d-flex justify-content-end mx-0 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=DY210" class="atc-bulk-button btn a___button-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> </div> </div> <div class="search_result atc-container pb-3" data-brand="rnd" data-category="Primary Antibodies" data-id="AF-410-NA" data-name="Human/Mouse TNF-alpha Antibody" data-position="10"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/antibodies/human-mouse-tnf-alpha-antibody_af-410-na" title="Human/Mouse TNF-alpha Antibody"> <h4>Human/Mouse TNF-alpha Antibody <span class="rounded align-middle p-1 badge best-seller" title="Best Seller">Best Seller</span> </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="R&D Systems, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-rnd-systems.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/antibodies/human-mouse-tnf-alpha-antibody_af-410-na" title="Human/Mouse TNF-alpha Antibody"> AF-410-NA </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/antibodies/human-mouse-tnf-alpha-antibody_af-410-na#reviews" class="searchImage_star4"> (5) </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/antibodies/human-mouse-tnf-alpha-antibody_af-410-na#citations"><span class="icon-icon-product-citations"></span>Citations (152)</a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/antibodies/human-mouse-tnf-alpha-antibody_af-410-na#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> <div class="d-none d-md-inline my-1"> <a href="#" class="search_result_images"><span class="icon-icon-image-camera-plus"></span> Images (8)</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> WB, IHC, CyTOF-ready, ICC, ICFlow, Neut </div> <div class="d-none d-md-block"> <span class="species_title"><strong>Reactivity:</strong></span> Human, Mouse </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-10" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-10"> <div class="row"> <div class="search_result_description col-xs-12 col-md-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Reactivity:</strong></td> <td class="pb-1 w-75 text-break"> Human, Mouse </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Details:</strong></td> <td class="pb-1 w-75 text-break"> Goat IgG Polyclonal </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> WB, IHC, CyTOF-ready, ICC, ICFlow, +1 More </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="af-410-na"></div> </div> <div class="search_result_image_wrapper col-xs-12 col-md-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="rnd"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src=https://resources.bio-techne.com/images/products/antibody/TNFalpha_AF410NA_Western_Blot_22812.jpg data-srcset=https://resources.bio-techne.com/images/products/antibody/TNFalpha_AF410NA_Western_Blot_22812.jpg alt="Detection of Human TNF-a antibody by Western Blot." title="Detection of Human TNF‑ alpha by Western Blot." data-caption="Western blot shows conditioned media from CHO Chinese hamster ovary cell line either mock transfected or transfected with human TNF- alpha. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). Specific bands were detected for TNF-a at approximately 14 and 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." data-badges="" data-application="Western Blot" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> <span class="image-count-text align-middle">(8)</span> </div> </div> </div> <i data-src="https://resources.bio-techne.com/images/products/antibody/TNFalpha_AF410NA_Western_Blot_22813.jpg" data-alt="Detection of Mouse TNF-a antibody by Western Blot." title="Detection of Mouse TNF‑ alpha by Western Blot." data-caption="Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 4 hours. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). Specific bands were detected for TNF-a at approximately 14 and 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/antibody/TNF-alpha_AF-410-NA_Immunocytochemistry_9636.jpg" data-alt="TNF-a antibody in RAW 264 by Immunocytochemistry (ICC).7 Mouse Cell Line by Immunocytochemistry (ICC)." title="TNF‑ alpha in RAW 264.7 Mouse Cell Line." data-caption="TNF-a was detected in immersion fixed RAW 264.7 mouse monocyte/ macrophage cell line treated with LPS using Human/Mouse TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." data-badges="<span class="biological-validation"></span>" data-application="Immunocytochemistry" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/antibody/TNF-alpha_AF-410-NA_Immunocytochemistry_7296.jpg" data-alt="TNF-a antibody in Mouse T Cells by Immunocytochemistry (ICC)." title="TNF‑ alpha in Mouse T Cells." data-caption="TNF‑ alpha was detected in immersion fixed activated mouse T Cells using 15 µg/mL Human/Mouse TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑410‑NA) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." data-badges="" data-application="Immunocytochemistry" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/antibody/af-410-na_mouse-tnf-alpha-affinity-purified-polyclonal-ab-immunohistochemistry-3520201707.jpg" data-alt="TNF-alpha antibody in Mouse Spleen Tissue by Immunohistochemistry (IHC-P)." title="TNF-alpha in Mouse Spleen Tissue." data-caption="TNF-alpha was detected in immersion fixed paraffin-embedded sections of mouse spleen tissue using Goat Anti-Human/Mouse TNF-alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. Staining was performed using our IHC Staining with VisUCyte HRP Polymer Detection Reagents." data-badges="" data-application="Immunohistochemistry" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/antibody/TNF-alpha_AF-410-NA_Block_Neutralize_8706.jpg" data-alt="Cytotoxicity Induced by TNF-alpha and Neutralization by Mouse TNF-alpha Antibody." title="Cytotoxicity Induced by TNF-alpha and Neutralization by Mouse TNF-alpha Antibody." data-caption="Recombinant Mouse TNF-a (410-MT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Mouse TNF-a (0.1 ng/mL) is neutralized (green line) by increasing concentrations of Human/Mouse TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA). The ND<SUB>50</SUB> is typically 1.5-10 ng/mL in the presence of the metabolic inhibitor actinomycin D." data-badges="" data-application="Neutralization" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/antibody/af-410-na_mouse-tnf-alpha-affinity-purified-polyclonal-ab-immunohistochemistry-3152023132230.jpg" data-alt="" title="Detection of TNF-alpha in Human Spleen." data-caption="TNF-alpha was detected in immersion fixed paraffin-embedded sections of Human Spleen using Goat Anti-Human/Mouse TNF-alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." data-badges="" data-application="Immunohistochemistry" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/af-410-na_mouse-tnf-alpha-affinity-purified-polyclonal-ab-41202410331929.jpg" data-alt="Detection of Mouse TNF-alpha by Western Blot" title="Detection of Mouse TNF-alpha by Western Blot" data-caption="WNT-5A induces a proinflammatory transformation in mouse microglia. (A, B) iNOS, COX-2 and TNF alpha were detected by immunoblotting in lysates from mouse primary microglia after WNT-5A stimulation (ctrl, 300 ng/ml, 6 hours). (B’) shows TNF alpha levels in the supernatant of primary microglia under ctrl conditions and upon WNT-5A stimulation (ctrl, 300 ng/ml, 24 hours; n = 4). At least three experiments are summarized in the bar graphs. Data are normalized to ctrl. Error bars give s.e.m. (C) Microglial proliferation was assessed by an MTT assay monitoring mitochondrial activity, which is proportional to cell number [see Additional file 1: Figure S5]. Stimulation with WNT-5A (300 ng/ml, 24 hours) increased MTT, which was blocked by PTX (100 ng/ml, overnight) or the MEK1/2 (10 μM) inhibitor, SL327. (D). Experiments were done in triplicate and data from three independent experiments are shown. *, P " data-badges="" data-application="Western Blot" data-width="250" data-height="250" class="none"></i> </div> </div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-AF-410-NA" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="AF-410-NA"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart-AF-410-NA" class="atc-lines"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="AF-410-NA"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> AF-410-NA </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="AF-410-SP"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> AF-410-SP </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> </div> <div class="atc-control ProductCart-AF-410-NA-control text-right"> <div class="row d-flex justify-content-end mx-0 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=AF-410-NA" class="atc-bulk-button btn a___button-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> </div> </div> <div class="search_result atc-container pb-3" data-brand="rnd" data-category="ELISA Kits" data-id="DY510" data-name="Rat TNF-alpha DuoSet ELISA" data-position="11"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/elisa-kits/rat-tnf-alpha-duoset-elisa_dy510" title="Rat TNF-alpha DuoSet ELISA"> <h4>Rat TNF-alpha DuoSet ELISA </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="R&D Systems, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-rnd-systems.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/elisa-kits/rat-tnf-alpha-duoset-elisa_dy510" title="Rat TNF-alpha DuoSet ELISA"> DY510 </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/elisa-kits/rat-tnf-alpha-duoset-elisa_dy510#reviews" class="searchImage_star4"> (7) </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/elisa-kits/rat-tnf-alpha-duoset-elisa_dy510#citations"><span class="icon-icon-product-citations"></span>Citations (184)</a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/elisa-kits/rat-tnf-alpha-duoset-elisa_dy510#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> <div class="d-none d-md-inline my-1"> <a href="#" class="search_result_images"><span class="icon-icon-image-camera-plus"></span> Images (1)</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> ELISA </div> <div class="d-none d-md-block"> </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-11" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-11"> <div class="row"> <div class="search_result_description col-xs-12 col-md-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Assay Range:</strong></td> <td class="pb-1 w-75 text-break"> 62.5 - 4,000 pg/mL </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> ELISA </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="dy510"></div> </div> <div class="search_result_image_wrapper col-xs-12 col-md-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="rnd"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src=https://resources.bio-techne.com/images/products/elisa/DATA_TNFalpha_DY510_ELISA_2079.jpg data-srcset=https://resources.bio-techne.com/images/products/elisa/DATA_TNFalpha_DY510_ELISA_2079.jpg alt="Rat TNF-alpha ELISA Standard Curve" title="Rat TNF-alpha ELISA Standard Curve" data-caption="" data-badges="" data-application="N/A" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-DY510" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="DY510"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart-DY510" class="atc-lines"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="DY510"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> DY510 </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="DY510-05"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> DY510-05 </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> </div> <div class="atc-control ProductCart-DY510-control text-right"> <div class="row d-flex justify-content-end mx-0 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=DY510" class="atc-bulk-button btn a___button-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> </div> </div> <div class="search_result atc-container pb-3" data-brand="novus" data-category="Primary Antibodies" data-id="NBP1-19532" data-name="TNF-alpha Antibody - BSA Free" data-position="12"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/antibodies/tnf-alpha-antibody_nbp1-19532" title="TNF-alpha Antibody - BSA Free"> <h4>TNF-alpha Antibody - BSA Free </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="Novus Biologicals, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-novus-biologicals.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/antibodies/tnf-alpha-antibody_nbp1-19532" title="TNF-alpha Antibody - BSA Free"> NBP1-19532 </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/antibodies/tnf-alpha-antibody_nbp1-19532#reviews" class="searchImage_star5"> (3) </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/antibodies/tnf-alpha-antibody_nbp1-19532#citations"><span class="icon-icon-product-citations"></span>Citations (84)</a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/antibodies/tnf-alpha-antibody_nbp1-19532#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> <div class="d-none d-md-inline my-1"> <a href="#" class="search_result_images"><span class="icon-icon-image-camera-plus"></span> Images (7)</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> IHC, WB, ICC/IF, Flow </div> <div class="d-none d-md-block"> <span class="species_title"><strong>Reactivity:</strong></span> Human, Mouse, Rat, Canine, Fish, +1 More </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-12" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-12"> <div class="row"> <div class="search_result_description col-xs-12 col-md-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Reactivity:</strong></td> <td class="pb-1 w-75 text-break"> Human, Mouse, Rat, Canine, Fish, +1 More </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Details:</strong></td> <td class="pb-1 w-75 text-break"> Rabbit IgG Polyclonal </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> IHC, WB, ICC/IF, Flow </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="nbp1-19532"></div> </div> <div class="search_result_image_wrapper col-xs-12 col-md-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="novus"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src=https://resources.bio-techne.com/images/products/TNF-alpha-Antibody-Western-Blot-NBP1-19532-img0007.jpg data-srcset=https://resources.bio-techne.com/images/products/TNF-alpha-Antibody-Western-Blot-NBP1-19532-img0007.jpg alt="Western Blot: TNF-alpha AntibodyBSA Free [NBP1-19532]" title="Western Blot: TNF-alpha AntibodyBSA Free [NBP1-19532]" data-caption="Western Blot: TNF-alpha Antibody [NBP1-19532] - Recombinant human TNF alpha (10 ng) was separated on a 12% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-TNF alpha in 5% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence." data-badges="" data-application="Western Blot" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> <span class="image-count-text align-middle">(7)</span> </div> </div> </div> <i data-src="https://resources.bio-techne.com/images/products/TNF-alpha-Antibody-Western-Blot-NBP1-19532-img0008.jpg" data-alt="Western Blot: TNF-alpha AntibodyBSA Free [NBP1-19532]" title="Western Blot: TNF-alpha AntibodyBSA Free [NBP1-19532]" data-caption="Western Blot: TNF-alpha Antibody [NBP1-19532] - Scutellarin attenuates hypertension-induced brain expression of NF-kappaB, TNF-alpha, IL-1beta, and IL-18. Western immunoblot analysis for TNF-alpha in the rat cortex and striatum. Treatment with scutellarin significantly reduced the expression of these inflammatory markers in a dose-dependent manner. *P < 0.001 versus sham group; #P < 0.05, P < 0.001 versus NS group; &P < 0.001 versus low-dose group. Image collected and cropped by CiteAb from the following publication (https://www.hindawi.com/journals/mi/2013/432623/), licensed under a CC-BY license." data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/TNF-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-19532-img0005.jpg" data-alt="Immunohistochemistry-Paraffin: TNF-alpha Antibody - BSA Free [NBP1-19532]" title="Immunohistochemistry-Paraffin: TNF-alpha Antibody - BSA Free [NBP1-19532]" data-caption="Immunohistochemistry-Paraffin: TNF-alpha Antibody [NBP1-19532] - Analysis of a FFPE tissue section of mouse intestine using TNF-alpha antibody (NBP1-19532) at 1:300 dilution. The binding of this primary antibody to TNF-alpha protein in the section was detected using HRP-labeled secondary antibody and DAB reagent, and nuclei of cells were counterstained using hematoxylin. This TNF-alpha antibody generated an expected diffused immunostaining of this protein in the tested tissue. Staining was primarily observed in the epithelial cells and some cells showed membrane positivity also." data-badges="" data-application="Immunohistochemistry-Paraffin" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/TNF-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-19532-img0006.jpg" data-alt="Immunohistochemistry-Paraffin: TNF-alpha Antibody - BSA Free [NBP1-19532]" title="Immunohistochemistry-Paraffin: TNF-alpha Antibody - BSA Free [NBP1-19532]" data-caption="Immunohistochemistry-Paraffin: TNF-alpha Antibody [NBP1-19532] - Analysis of a FFPE tissue section of mouse intestine using TNF-alpha antibody (NBP1-19532) at 1:300 dilution. The binding of this primary antibody to TNF-alpha protein in the section was detected using HRP-labeled secondary antibody and DAB reagent, and nuclei of cells were counterstained using hematoxylin. This TNF-alpha antibody generated an expected immunopositivity of this protein in the tested tissue. Staining was primarily observed in the epithelial cells while some staining was present in mucosa muscularis also." data-badges="" data-application="Immunohistochemistry-Paraffin" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nbp1-19532_rabbit-polyclonal-tnf-alpha-antibody-27122023126198.jpg" data-alt="TNF-alpha Antibody - BSA Free" title="Western Blot: TNF-alpha Antibody - BSA Free [NBP1-19532] -" data-caption="Scutellarin attenuates hypertension-induced brain expression of NF-kappa B, TNF-alpha, IL-1 beta, and IL-18. Western immunoblot analysis for (a) NF-kappa B p65, (b) TNF-alpha, (c) IL-1 beta, and (d) IL-18 in the rat cortex and striatum. Treatment with scutellarin significantly reduced the expression of these inflammatory markers in a dose-dependent manner. *P < 0.001 versus sham group; #P < 0.05, ◆P < 0.001 versus NS group; &P < 0.001 versus low-dose group." data-badges="<span class="biological-validation"></span>" data-application="Western Blot" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nbp1-19532_rabbit-polyclonal-tnf-alpha-antibody-31020241618927.jpg" data-alt="TNF-alpha Antibody - BSA Free" title="Immunocytochemistry/ Immunofluorescence: TNF-alpha Antibody - BSA Free [NBP1-19532] -" data-caption="Immunocytochemistry/ Immunofluorescence: TNF-alpha Antibody - BSA Free [NBP1-19532] - Effects of femoral artery occlusion on TNF-alpha signal in sensory nerves. (A) Seventy-two hours of femoral artery occlusion increased the levels of TNF-alpha in the DRG tissues as compared with them in DRG of control limbs (n = 6 in each group); & PTX given into the hindlimb muscles attenuated amplification of TNF-alpha in the DRG tissues of limbs with femoral artery occlusion (n = 8). ∗P < 0.05, occlusion group vs. control & occlusion with prior PTX. (B) Immunofluorescence was used to examine double-labeling for TNF-alpha & peripherin/NF-200 (n = 3). Peripherin was used to label DRG neurons that project thin C-fibers. NF200 was used to identify A-fibers of DRG neurons. Representative photomicrographs show co-existence of TNF-alpha & peripherin staining in DRG neurons (top panel), whereas few TNF-alpha & NF-200 staining were observed in DRG neurons (bottom panel). Arrows indicate representative positive cells for both TNF-alpha & peripherin after they were merged. Scale bar = 50 μm. (C) Representative bands (left panel) & averaged data (right panel), demonstrating that femoral artery occlusion upregulated protein expression TNF-alpha receptor subtype TNFR1, but not TNFR2. A significant difference in TNFR1 was seen between control & occluded groups. ∗P < 0.05 vs. control. n = 6 in each group. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30374312), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/nbp1-19532_rabbit-polyclonal-tnf-alpha-antibody-310202416171446.jpg" data-alt="TNF-alpha Antibody - BSA Free" title="Immunocytochemistry/ Immunofluorescence: TNF-alpha Antibody - BSA Free [NBP1-19532] -" data-caption="Immunocytochemistry/ Immunofluorescence: TNF-alpha Antibody - BSA Free [NBP1-19532] - Immunoflorescence staining results of TNF-alpha (96 h). A). Lamella of gill tissue (arrow). Control group. IF. 20 μm. B) Positive reaction in lamellar cells of gill tissue (arrows). Low dose toxic group. IF. 20 μm. C) Positivity in lamellar cells of gill tissue (arrow). High dose toxic group. IF. 20 μm. D) Liver tissue, vena centralis (arrow). Control group. IF. 20 μm. E) Positive reactions in hepatocytes (arrows). Low dose toxic group. IF. 20 μm. F) Positive reactions in hepatocytes (arrows). High dose toxic group. IF. 20 μm. G) Weak positive reactions in neurons (arrows). Control group. IF. 20 μm. H) Weak positivity in neurons (arrows). Low dose toxic group. IF. 20 μm. I) Positivity in neurons (arrows). High dose toxic group. IF. 20 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29321977), licensed under a CC-BY license. Not internally tested by Novus Biologicals." data-badges="" data-application="Immunocytochemistry/ Immunofluorescence" data-width="250" data-height="250" class="none"></i> </div> </div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-NBP1-19532" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="NBP1-19532"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart-NBP1-19532" class="atc-lines"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="NBP1-19532"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> NBP1-19532 </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="NBP1-19532SS"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> NBP1-19532SS </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> </div> <div class="atc-control ProductCart-NBP1-19532-control text-right"> <div class="row d-flex justify-content-end mx-0 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=NBP1-19532" class="atc-bulk-button btn a___button-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> </div> </div> <div class="search_result atc-container pb-3" data-brand="rnd" data-category="Primary Antibodies" data-id="MAB610" data-name="Human TNF-alpha Antibody" data-position="0"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/antibodies/human-tnf-alpha-antibody-28401_mab610" title="Human TNF-alpha Antibody"> <h4>Human TNF-alpha Antibody <span class="rounded align-middle p-1 badge best-seller" title="Best Seller">Best Seller</span> </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="R&D Systems, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-rnd-systems.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/antibodies/human-tnf-alpha-antibody-28401_mab610" title="Human TNF-alpha Antibody"> MAB610 </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/antibodies/human-tnf-alpha-antibody-28401_mab610#reviews" class="searchImage_star5"> (4) </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/antibodies/human-tnf-alpha-antibody-28401_mab610#citations"><span class="icon-icon-product-citations"></span>Citations (121)</a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/antibodies/human-tnf-alpha-antibody-28401_mab610#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> <div class="d-none d-md-inline my-1"> <a href="#" class="search_result_images"><span class="icon-icon-image-camera-plus"></span> Images (3)</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> WB, ICC, Neut </div> <div class="d-none d-md-block"> <span class="species_title"><strong>Reactivity:</strong></span> Human </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-0" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-0"> <div class="row"> <div class="search_result_description col-xs-12 col-md-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Reactivity:</strong></td> <td class="pb-1 w-75 text-break"> Human </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Details:</strong></td> <td class="pb-1 w-75 text-break"> Mouse IgG1 Monoclonal Clone #28401 </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> WB, ICC, Neut </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="mab610"></div> </div> <div class="search_result_image_wrapper col-xs-12 col-md-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="rnd"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src=https://resources.bio-techne.com/images/products/antibody/TNFalpha_MAB610_Western_Blot_21055.jpg data-srcset=https://resources.bio-techne.com/images/products/antibody/TNFalpha_MAB610_Western_Blot_21055.jpg alt="Detection of Recombinant Human TNF-a antibody by Western Blot." title="Detection of Recombinant Human TNF‑ alpha by Western Blot." data-caption="Western blot shows 25 ng of Recombinant Human TNF-a (Catalog # 210-TA), Recombinant Mouse TNF-a aa 80-235 (Catalog # 410-MT), Recombinant Rat TNF-a (Catalog # 510-RT), and Recombinant Human APRIL/TNFSF13 (Catalog # 5860-AP). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human TNF-a Monoclonal Antibody (Catalog # MAB610) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TNF-a at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." data-badges="" data-application="Western Blot" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> <span class="image-count-text align-middle">(3)</span> </div> </div> </div> <i data-src="https://resources.bio-techne.com/images/products/antibody/TNF-alpha_MAB610_Immunocytochemistry_9626.jpg" data-alt="TNF-a antibody in Human PBMCs by Immunocytochemistry (ICC)." title="TNF‑ alpha in Human PBMCs." data-caption="TNF-a was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with LPS and monensin using Mouse Anti-Human TNF-a Monoclonal Antibody (Catalog # MAB610) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." data-badges="" data-application="Immunocytochemistry" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/antibody/TNFalpha_MAB610_Block_Neutralize_14609.jpg" data-alt="Cytotoxicity Induced by TNF‑ alpha and Neutralization by Human TNF‑ alpha Antibody." title="Cytotoxicity Induced by TNF‑ alpha and Neutralization by Human TNF‑ alpha Antibody." data-caption="Recombinant Human TNF-a (Catalog # 210-TA) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Human TNF-a (0.25 ng/mL) is neutral-ized (green line) by increasing concentrations of Mouse Anti-Human TNF-a Monoclonal Antibody (Catalog # MAB610). The ND<SUB>50</SUB> is typically 0.01-0.04 µg/mL in the presence of the metabolic inhibitor actinomycin D." data-badges="" data-application="Neutralization" data-width="250" data-height="250" class="none"></i> </div> </div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-MAB610" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="MAB610"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart-MAB610" class="atc-lines"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="MAB610-100"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> MAB610-100 </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="MAB610-500"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> MAB610-500 </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="MAB610-SP"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> MAB610-SP </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> </div> <div class="atc-control ProductCart-MAB610-control text-right"> <div class="row d-flex justify-content-end mx-0 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=MAB610" class="atc-bulk-button btn a___button-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> </div> </div> <div class="search_result atc-container pb-3" data-brand="rnd" data-category="ELISA Kits" data-id="PTA00" data-name="Porcine TNF-alpha Quantikine ELISA Kit" data-position="14"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/elisa-kits/porcine-tnf-alpha-quantikine-elisa-kit_pta00" title="Porcine TNF-alpha Quantikine ELISA Kit"> <h4>Porcine TNF-alpha Quantikine ELISA Kit <span class="rounded align-middle p-1 badge best-seller" title="Best Seller">Best Seller</span> </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="R&D Systems, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-rnd-systems.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/elisa-kits/porcine-tnf-alpha-quantikine-elisa-kit_pta00" title="Porcine TNF-alpha Quantikine ELISA Kit"> PTA00 </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/elisa-kits/porcine-tnf-alpha-quantikine-elisa-kit_pta00#reviews" class="searchImage_star5"> (2) </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/elisa-kits/porcine-tnf-alpha-quantikine-elisa-kit_pta00#citations"><span class="icon-icon-product-citations"></span>Citations (149)</a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/elisa-kits/porcine-tnf-alpha-quantikine-elisa-kit_pta00#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> <div class="d-none d-md-inline my-1"> <a href="#" class="search_result_images"><span class="icon-icon-image-camera-plus"></span> Images (3)</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> ELISA </div> <div class="d-none d-md-block"> </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-14" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-14"> <div class="row"> <div class="search_result_description col-xs-12 col-md-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Sensitivity:</strong></td> <td class="pb-1 w-75 text-break"> 5 pg/mL </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> ELISA </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Assay Range:</strong></td> <td class="pb-1 w-75 text-break"> 23.4 - 1,500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma) </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="pta00"></div> </div> <div class="search_result_image_wrapper col-xs-12 col-md-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="rnd"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src=https://resources.bio-techne.com/images/products/elisa/DATA_TNFalpha_PTA00_ELISA_1061.jpg data-srcset=https://resources.bio-techne.com/images/products/elisa/DATA_TNFalpha_PTA00_ELISA_1061.jpg alt="Porcine TNF-alpha ELISA Cell Culture Supernate Standard Curve" title="Porcine TNF-alpha ELISA Cell Culture Supernate Standard Curve" data-caption="" data-badges="" data-application="N/A" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> <span class="image-count-text align-middle">(3)</span> </div> </div> </div> <i data-src="https://resources.bio-techne.com/images/products/elisa/DATA_TNFalpha_PTA00_ELISA_1062.jpg" data-alt="Porcine TNF-alpha ELISA Serum/Plasma Standard Curve" title="Porcine TNF-alpha ELISA Serum/Plasma Standard Curve" data-caption="" data-badges="" data-application="N/A" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/elisa/TNF-alpha_PTA00_ELISA_343.jpg" data-alt="Porcine TNF-alpha ELISA Linearity" title="Porcine TNF-alpha ELISA Linearity" data-caption="" data-badges="" data-application="N/A" data-width="250" data-height="250" class="none"></i> </div> </div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-PTA00" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="PTA00"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart-PTA00" class="atc-lines"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="PTA00"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> PTA00 </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> </div> <div class="atc-control ProductCart-PTA00-control text-right"> <div class="row d-flex justify-content-end mx-0 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=PTA00" class="atc-bulk-button btn a___button-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> </div> </div> <div class="search_result atc-container pb-3" data-brand="rnd" data-category="Proteins and Enzymes" data-id="10291-TA" data-name="Recombinant Human TNF-alpha (HEK293-expressed) Protein, CF" data-position="15"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/proteins-enzymes/recombinant-human-tnf-alpha-hek293-expressed-protein-cf_10291-ta" title="Recombinant Human TNF-alpha (HEK293-expressed) Protein, CF"> <h4>Recombinant Human TNF-alpha (HEK293-expressed) Protein, CF <span class="rounded align-middle p-1 badge best-seller" title="Best Seller">Best Seller</span> </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="R&D Systems, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-rnd-systems.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/proteins-enzymes/recombinant-human-tnf-alpha-hek293-expressed-protein-cf_10291-ta" title="Recombinant Human TNF-alpha (HEK293-expressed) Protein, CF"> 10291-TA </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/proteins-enzymes/recombinant-human-tnf-alpha-hek293-expressed-protein-cf_10291-ta#reviews" class="searchImage_star5"> (4) </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/proteins-enzymes/recombinant-human-tnf-alpha-hek293-expressed-protein-cf_10291-ta#citations"><span class="icon-icon-product-citations"></span>Citations (15)</a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/proteins-enzymes/recombinant-human-tnf-alpha-hek293-expressed-protein-cf_10291-ta#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> <div class="d-none d-md-inline my-1"> <a href="#" class="search_result_images"><span class="icon-icon-image-camera-plus"></span> Images (3)</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> BA </div> <div class="d-none d-md-block"> </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-15" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="row"> <div class="align-middle mt-2 col-xs-12 col-sm-9"> <p class="mb-0">Analyzed by SEC-MALS</p> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-15"> <div class="row"> <div class="search_result_description col-xs-12 col-md-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Source:</strong></td> <td class="pb-1 w-75 text-break"> HEK293 </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Accession #:</strong></td> <td class="pb-1 w-75 text-break"> P01375 </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> BA </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="10291-ta"></div> </div> <div class="search_result_image_wrapper col-xs-12 col-md-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="rnd"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src=https://resources.bio-techne.com/images/products/10291-ta_recombinant-human-tnf-alpha-hek293-expressed-protein-cf-sec-mals-44202312253.jpg data-srcset=https://resources.bio-techne.com/images/products/10291-ta_recombinant-human-tnf-alpha-hek293-expressed-protein-cf-sec-mals-44202312253.jpg alt="" title="Recombinant Human TNF-alpha (HEK293-expressed) Protein SEC-MALS" data-caption="Recombinant human TNF-alpha (Catalog # 10291-TA) has a molecular weight (MW) of 52.3 kDa as analyzed by SEC-MALS, suggesting that this protein is a homotrimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation)." data-badges="" data-application="SEC-MALS" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> <span class="image-count-text align-middle">(3)</span> </div> </div> </div> <i data-src="https://resources.bio-techne.com/images/products/protein/TNFalpha_10291TA_2813.jpg" data-alt="Recombinant Human TNF-alpha (HEK293-expressed) Protein Bioactivity" title="Recombinant Human TNF-alpha (HEK293-expressed) Protein Bioactivity" data-caption="Recombinant Human TNF‑ alpha (HEK293-expressed) (Catalog # 10291-TA) induces cytotoxicity in the L‑929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED<sub>50</sub> for this effect is 20‑100 pg/mL." data-badges="" data-application="Bioactivity" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/protein/TNFalpha_10291TA_2753.jpg" data-alt="Recombinant Human TNF-alpha (HEK293-expressed) Protein SDS-PAGE" title="Recombinant Human TNF-alpha (HEK293-expressed) Protein SDS-PAGE" data-caption="2 μg/lane of Recombinant Human TNF-alpha (HEK293-expressed) (Catalog # 10291-TA) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 14-18 kDa." data-badges="" data-application="SDS-Page" data-width="250" data-height="250" class="none"></i> </div> </div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-10291-TA" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="10291-TA"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart-10291-TA" class="atc-lines"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="10291-TA-01M"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> 10291-TA-01M </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="10291-TA-020"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> 10291-TA-020 </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="10291-TA-050"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> 10291-TA-050 </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="10291-TA-100"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> 10291-TA-100 </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="10291-TA-250"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> 10291-TA-250 </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="10291-TA-500"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> 10291-TA-500 </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> </div> <div class="atc-control ProductCart-10291-TA-control text-right"> <div class="row d-flex justify-content-end mx-0 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=10291-TA" class="atc-bulk-button btn a___button-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> </div> </div> <div class="search_result atc-container pb-3" data-brand="rnd" data-category="Proteome Profiler Antibody Arrays" data-id="ARY006" data-name="Proteome Profiler Mouse Cytokine Array Kit, Panel A" data-position="24"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/antibody-arrays/proteome-profiler-mouse-cytokine-array-kit-panel-a_ary006" title="Proteome Profiler Mouse Cytokine Array Kit, Panel A"> <h4>Proteome Profiler Mouse Cytokine Array Kit, Panel A <span class="rounded align-middle p-1 badge best-seller" title="Best Seller">Best Seller</span> </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="R&D Systems, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-rnd-systems.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/antibody-arrays/proteome-profiler-mouse-cytokine-array-kit-panel-a_ary006" title="Proteome Profiler Mouse Cytokine Array Kit, Panel A"> ARY006 </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/antibody-arrays/proteome-profiler-mouse-cytokine-array-kit-panel-a_ary006#reviews" class="searchImage_star5"> (36) </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/antibody-arrays/proteome-profiler-mouse-cytokine-array-kit-panel-a_ary006#citations"><span class="icon-icon-product-citations"></span>Citations (685)</a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/antibody-arrays/proteome-profiler-mouse-cytokine-array-kit-panel-a_ary006#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> <div class="d-none d-md-inline my-1"> <a href="#" class="search_result_images"><span class="icon-icon-image-camera-plus"></span> Images (3)</a> </div> </div> <div class="d-none d-md-block"> </div> <div class="d-none d-md-block"> </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-24" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="row"> <div class="align-middle mt-2 col-xs-12 col-sm-9"> <p class="mb-0">Contains 4 membranes - each spotted in duplicate with 40 different cytokine antibodies</p> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-24"> <div class="row"> <div class="search_result_description col-xs-12 col-md-9"> <div class="compare_tool_select form-inline mb-2" data-sku="ary006"></div> </div> <div class="search_result_image_wrapper col-xs-12 col-md-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="rnd"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src=https://resources.bio-techne.com/images/products/ary006_proteome-profiler-mouse-cytokine-array-kit-panel-a-5112020135828.png data-srcset=https://resources.bio-techne.com/images/products/ary006_proteome-profiler-mouse-cytokine-array-kit-panel-a-5112020135828.png alt="The Mouse Cytokine Array, Panel A utilizes capture antibodies spotted onto a nitrocellulose membrane to allow high-throughput multi-analyte profiling of 40 cytokines, chemokines, and more in a single sample." title="The Mouse Cytokine Array, Panel A utilizes capture antibodies spotted onto a nitrocellulose membrane to allow high-throughput multi-analyte profiling of 40 cytokines, chemokines, and more in a single sample." data-caption="Mouse splenocytes treated with 100 ng/mL LPS for 20 hours were mixed with a cocktail of biotinylated detection antibodies, and then incubated with the Mouse Cytokine Array. The array was then incubated with streptavidin-horseradish peroxidase followed by chemiluminescent detection. After detection, the array data were quantitated to generate a protein profile (histogram). The table shows the analytes detected and their location on the membrane." data-badges="" data-application="N/A" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> <span class="image-count-text align-middle">(3)</span> </div> </div> </div> <i data-src="https://resources.bio-techne.com/images/products/ary006_proteome-profiler-mouse-cytokine-array-kit-panel-a-912202013227.jpg" data-alt="The Mouse Cytokine Array detects multiple analytes in cell culture lysates and supernates." title="The Mouse Cytokine Array detects multiple analytes in cell culture lysates and supernates." data-caption="(A. and B.) Lysates of RAW264.7 mouse monocyte/macrophage cell line untreated or treated with 100 ng/mL LPS for 24 hours. 100 µg of lysate was used for each array shown. (C. and D.) Supernates of RAW264.7 mouse monocyte/macrophage cell line untreated or treated with 100 ng/mL LPS for 24 hours. 100 µL of supernate was used for each array shown. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System." data-badges="" data-application="N/A" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/ary006_proteome-profiler-mouse-cytokine-array-kit-panel-a-912202013318.jpg" data-alt="The Mouse Cytokine Array detects multiple analytes in mouse serum and tissue lysates." title="The Mouse Cytokine Array detects multiple analytes in mouse serum and tissue lysates." data-caption="(A and B.) Samples of mouse serum from two different vendors. 200 µL of serum was used for each array shown. (C. and D.) Lysates of mouse lung tissue and mouse stomach tissue. 200 µg of lysate was used for each array shown. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System." data-badges="" data-application="N/A" data-width="250" data-height="250" class="none"></i> </div> </div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-ARY006" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="ARY006"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart-ARY006" class="atc-lines"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="ARY006"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> ARY006 </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> </div> <div class="atc-control ProductCart-ARY006-control text-right"> <div class="row d-flex justify-content-end mx-0 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=ARY006" class="atc-bulk-button btn a___button-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> </div> </div> <div class="search_result atc-container pb-3" data-brand="proteinsimple" data-category="Simple Plex" data-id="894977" data-name="Simple Plex Control for Human TNF-alpha" data-position="17"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/simple-plex/simple-plex-control-for-human-tnf-alpha_894977" title="Simple Plex Control for Human TNF-alpha"> <h4>Simple Plex Control for Human TNF-alpha </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="ProteinSimple, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-proteinsimple.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/simple-plex/simple-plex-control-for-human-tnf-alpha_894977" title="Simple Plex Control for Human TNF-alpha"> 894977 </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/simple-plex/simple-plex-control-for-human-tnf-alpha_894977#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> Ctrl, Simple Plex </div> <div class="d-none d-md-block"> </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-17" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="row"> <div class="align-middle mt-2 col-xs-12 col-sm-9"> <p class="mb-0">Control for use with Human and Non-Human Primate TNF-alpha Simple Plex Cartridge Kit</p> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-17"> <div class="row"> <div class="search_result_description col-xs-12 col-sm-12"> </div> </div> <div class="row"> <div class="search_result_product_details col-xs-12 col-sm-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> Ctrl, Simple Plex </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="894977"></div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-894977" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="894977"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart-894977" class="atc-lines"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="894977"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> 894977 </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> </div> <div class="atc-control ProductCart-894977-control text-right"> <div class="row d-flex justify-content-end mx-0 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=894977" class="atc-bulk-button btn a___button-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> </div> </div> <div class="search_result atc-container pb-3" data-brand="rnd" data-category="Proteins and Enzymes" data-id="510-RT" data-name="Recombinant Rat TNF-alpha Protein" data-position="18"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/proteins-enzymes/recombinant-rat-tnf-alpha-protein_510-rt" title="Recombinant Rat TNF-alpha Protein"> <h4>Recombinant Rat TNF-alpha Protein </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="R&D Systems, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-rnd-systems.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/proteins-enzymes/recombinant-rat-tnf-alpha-protein_510-rt" title="Recombinant Rat TNF-alpha Protein"> 510-RT </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/proteins-enzymes/recombinant-rat-tnf-alpha-protein_510-rt#reviews" class="searchImage_star5"> (3) </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/proteins-enzymes/recombinant-rat-tnf-alpha-protein_510-rt#citations"><span class="icon-icon-product-citations"></span>Citations (100)</a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/proteins-enzymes/recombinant-rat-tnf-alpha-protein_510-rt#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> <div class="d-none d-md-inline my-1"> <a href="#" class="search_result_images"><span class="icon-icon-image-camera-plus"></span> Images (1)</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> BA </div> <div class="d-none d-md-block"> </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-18" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-18"> <div class="row"> <div class="search_result_description col-xs-12 col-md-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Source:</strong></td> <td class="pb-1 w-75 text-break"> E. coli </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Accession #:</strong></td> <td class="pb-1 w-75 text-break"> P16599 </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> BA </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="510-rt"></div> </div> <div class="search_result_image_wrapper col-xs-12 col-md-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="rnd"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src=https://resources.rndsystems.com/categoryimages/rndsystems_recombinant-proteins-enzymes.jpg data-srcset=https://resources.rndsystems.com/categoryimages/rndsystems_recombinant-proteins-enzymes.jpg alt="R&D Systems Recombinant Proteins and Enzymes" title="R&D Systems Recombinant Proteins and Enzymes" data-caption="" data-badges="" data-application="" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-510-RT" data-cf="1" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="510-RT"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div class="row"> <hr/> </div> <div class="col-12 py-2 font-weight-bold px-0"> Carrier Free <a href="#" data-toggle="tooltip" title="No added BSA"> <span class="icon-information-outline2 ml-1"></span> </a> </div> <div id="ProductCart-510-RT-cf" class="atc-lines type-cf"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="510-RT-050/CF"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> 510-RT-050/CF </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="510-RT-010/CF"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> 510-RT-010/CF </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> </div> <div class="row"> <hr/> </div> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="col-12 py-2 font-weight-bold px-0"> With Carrier <a href="#" data-toggle="tooltip" title="Formulated with BSA to improve stability"> <span class="icon-information-outline2 ml-1"></span> </a> </div> <div id="ProductCart-510-RT-ca" class="atc-lines type-ca"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="510-RT-010"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> 510-RT-010 </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="510-RT-050"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> 510-RT-050 </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> </div> <div class="atc-control ProductCart-510-RT-control text-right"> <div class="row d-flex justify-content-end mx-0 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=510-RT" class="atc-bulk-button btn a___button-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> </div> </div> <div class="search_result atc-container pb-3" data-brand="rnd" data-category="Proteome Profiler Antibody Arrays" data-id="ARY005B" data-name="Proteome Profiler Human Cytokine Array Kit" data-position="0"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/antibody-arrays/proteome-profiler-human-cytokine-array-kit_ary005b" title="Proteome Profiler Human Cytokine Array Kit"> <h4>Proteome Profiler Human Cytokine Array Kit <span class="rounded align-middle p-1 badge best-seller" title="Best Seller">Best Seller</span> </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="R&D Systems, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-rnd-systems.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/antibody-arrays/proteome-profiler-human-cytokine-array-kit_ary005b" title="Proteome Profiler Human Cytokine Array Kit"> ARY005B </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/antibody-arrays/proteome-profiler-human-cytokine-array-kit_ary005b#reviews" class="searchImage_star5"> (12) </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/antibody-arrays/proteome-profiler-human-cytokine-array-kit_ary005b#citations"><span class="icon-icon-product-citations"></span>Citations (501)</a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/antibody-arrays/proteome-profiler-human-cytokine-array-kit_ary005b#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> <div class="d-none d-md-inline my-1"> <a href="#" class="search_result_images"><span class="icon-icon-image-camera-plus"></span> Images (3)</a> </div> </div> <div class="d-none d-md-block"> </div> <div class="d-none d-md-block"> </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-0" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="row"> <div class="align-middle mt-2 col-xs-12 col-sm-9"> <p class="mb-0">Contains 4 membranes - each spotted in duplicate with 36 different cytokine antibodies</p> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-0"> <div class="row"> <div class="search_result_description col-xs-12 col-md-9"> <div class="compare_tool_select form-inline mb-2" data-sku="ary005b"></div> </div> <div class="search_result_image_wrapper col-xs-12 col-md-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="rnd"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src=https://resources.bio-techne.com/images/products/ary005b_proteome-profiler-human-cytokine-array-kit-51120201457.png data-srcset=https://resources.bio-techne.com/images/products/ary005b_proteome-profiler-human-cytokine-array-kit-51120201457.png alt="The Human Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates." title="The Human Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates." data-caption="The amount of cell culture supernate used on each array and the duration of exposure to X-ray film is indicated below. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software. 1A: THP-1 human acute monocytic leukemia cells were untreated or treated with 1 µg/mL LPS for 4 hours, then 5mM Nigericin (Tocris, Catalog # 4312) for 1 hour (50 µL of cell culture supernate, 10 minute exposure). 1B: SW480 human colorectal adenocarcinoma cells were untreated or treated with 100 ng/mL LPS for 24 hours (500 µL of cell culture supernate, 10 minute exposure)." data-badges="" data-application="N/A" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> <span class="image-count-text align-middle">(3)</span> </div> </div> </div> <i data-src="https://resources.bio-techne.com/images/products/ary005b_proteome-profiler-human-cytokine-array-kit-511202014644.png" data-alt="The Human Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell lysates." title="The Human Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell lysates." data-caption="The amount of cell lysate used on each array and the duration of exposure to X-ray film is indicated below. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software. 2A: SW1353 human chondrosarcoma cells were untreated or treated with 5 ng/mL recombinant human IL-1 alpha (R&D Systems, Catalog # 200-LA) and 10 ng/mL recombinant human OSM (R&D Systems, Catalog # 295-OM) for 24 hours (200 µg of cell lysate, 5 minute exposure). 2B: THP-1 human acute monocytic leukemia cells were untreated or treated with 1 µg/mL recombinant human IFN-gamma (R&D Systems, Catalog # 285-IF) for 8 hours, then 1 µg/mL LPS for 16 hours (200 µg of cell lysate, 5 minute exposure)." data-badges="" data-application="N/A" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/ary005b_proteome-profiler-human-cytokine-array-kit-511202014232.jpg" data-alt="The Human Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in tissue lysate samples." title="The Human Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in tissue lysate samples." data-caption="200 µg of tissue lysate was run on each array. Data shown are from a 5 minute exposure to X-ray film.<br><br>1. ICAM-1/CD54<br>2. MIF<br>3. Complment Component C5/C5a<br>4. CXCL1/GRO alpha <br>5. IL-8<br>6. IL-16<br>7. IL-18<br>8. CCL5/RANTES<br>9. IL-1ra/IL-1F3<br>10. Serpin E1/PAI-1" data-badges="" data-application="N/A" data-width="250" data-height="250" class="none"></i> </div> </div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-ARY005B" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="ARY005B"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart-ARY005B" class="atc-lines"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="ARY005B"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> ARY005B </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> </div> <div class="atc-control ProductCart-ARY005B-control text-right"> <div class="row d-flex justify-content-end mx-0 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=ARY005B" class="atc-bulk-button btn a___button-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> </div> </div> <div class="search_result atc-container pb-3" data-brand="rnd" data-category="Luminex Assays" data-id="FCSTM18B" data-name="Human XL Cytokine Luminex® Kit Performance Assay" data-position="0"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/luminex-assays/human-xl-cytokine-luminex-kit-performance-assay_fcstm18b" title="Human XL Cytokine Luminex® Kit Performance Assay"> <h4>Human XL Cytokine Luminex® Kit Performance Assay </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="R&D Systems, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-rnd-systems.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/luminex-assays/human-xl-cytokine-luminex-kit-performance-assay_fcstm18b" title="Human XL Cytokine Luminex® Kit Performance Assay"> FCSTM18B </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/luminex-assays/human-xl-cytokine-luminex-kit-performance-assay_fcstm18b#reviews" class="searchImage_star4"> (1) </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/luminex-assays/human-xl-cytokine-luminex-kit-performance-assay_fcstm18b#citations"><span class="icon-icon-product-citations"></span>Citations (5)</a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/luminex-assays/human-xl-cytokine-luminex-kit-performance-assay_fcstm18b#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> <div class="d-none d-md-inline my-1"> <a href="#" class="search_result_images"><span class="icon-icon-image-camera-plus"></span> Images (2)</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> ELISA </div> <div class="d-none d-md-block"> </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-0" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-0"> <div class="row"> <div class="search_result_description col-xs-12 col-md-9"> <p>Luminex High Performance Assays go through rigorous development, validation, and quality control testing to ensure you are getting the best performance out of your multiplex assay. Panels are customizable so you can order a selection of the analytes or the entire panel.</p> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> ELISA </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="fcstm18b"></div> </div> <div class="search_result_image_wrapper col-xs-12 col-md-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="rnd"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src=https://resources.bio-techne.com/images/products/fcstm18b_human-xl-cyt-premixed-mag-luminex-perf-assay-kit-226202217646.png data-srcset=https://resources.bio-techne.com/images/products/fcstm18b_human-xl-cyt-premixed-mag-luminex-perf-assay-kit-226202217646.png alt="Human Luminex Cytokine Assay Standard Curves" title="Human Luminex Cytokine Assay Standard Curves" data-caption="" data-badges="" data-application="N/A" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> <span class="image-count-text align-middle">(2)</span> </div> </div> </div> <i data-src="https://resources.bio-techne.com/images/products/fcstm18b_human-xl-cyt-premixed-mag-luminex-perf-assay-kit-226202217746.png" data-alt="Human Luminex Cytokine Assay Serum Sample Values" title="Human Luminex Cytokine Assay Serum Sample Values" data-caption="Serum samples from 20 apparently healthy individuals were run on the Human XL Cytokine Luminex High Performance Panel and sample values plotted. Minimum and maximum values shown by exterior lines, the box represents the 1st and 3rd quartiles with the center line representing the median." data-badges="" data-application="N/A" data-width="250" data-height="250" class="none"></i> </div> </div> </div> </div> <div class="mt-2 text-right"> </div> </div> </div> </div> <div class="search_result atc-container pb-3" data-brand="rnd" data-category="Primary Antibodies" data-id="MAB210" data-name="Human TNF-alpha Antibody" data-position="21"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/antibodies/human-tnf-alpha-antibody-1825_mab210" title="Human TNF-alpha Antibody"> <h4>Human TNF-alpha Antibody </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="R&D Systems, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-rnd-systems.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/antibodies/human-tnf-alpha-antibody-1825_mab210" title="Human TNF-alpha Antibody"> MAB210 </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/antibodies/human-tnf-alpha-antibody-1825_mab210#reviews" class="searchImage_star4"> (2) </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/antibodies/human-tnf-alpha-antibody-1825_mab210#citations"><span class="icon-icon-product-citations"></span>Citations (50)</a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/antibodies/human-tnf-alpha-antibody-1825_mab210#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> <div class="d-none d-md-inline my-1"> <a href="#" class="search_result_images"><span class="icon-icon-image-camera-plus"></span> Images (1)</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> Neut </div> <div class="d-none d-md-block"> <span class="species_title"><strong>Reactivity:</strong></span> Human </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-21" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-21"> <div class="row"> <div class="search_result_description col-xs-12 col-md-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Reactivity:</strong></td> <td class="pb-1 w-75 text-break"> Human </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Details:</strong></td> <td class="pb-1 w-75 text-break"> Mouse IgG1 Monoclonal Clone #1825 </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> Neut </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="mab210"></div> </div> <div class="search_result_image_wrapper col-xs-12 col-md-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="rnd"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src=https://resources.bio-techne.com/images/products/antibody/TNFalpha_MAB210_Block_Neutralize_14608.jpg data-srcset=https://resources.bio-techne.com/images/products/antibody/TNFalpha_MAB210_Block_Neutralize_14608.jpg alt="Cytotoxicity Induced by TNF‑ alpha and Neutralization by Human TNF‑ alpha Antibody." title="Cytotoxicity Induced by TNF‑ alpha and Neutralization by Human TNF‑ alpha Antibody." data-caption="Recombinant Human TNF-a (Catalog # 210-TA) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Human TNF-a (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TNF-a Monoclonal Antibody (Catalog # MAB210). The ND<SUB>50</SUB> is typically 0.01-0.06 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." data-badges="" data-application="Neutralization" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-MAB210" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="MAB210"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart-MAB210" class="atc-lines"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="MAB210-100"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> MAB210-100 </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="MAB210-500"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> MAB210-500 </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="MAB210-SP"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> MAB210-SP </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> </div> <div class="atc-control ProductCart-MAB210-control text-right"> <div class="row d-flex justify-content-end mx-0 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=MAB210" class="atc-bulk-button btn a___button-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> </div> </div> <div class="search_result atc-container pb-3" data-brand="rnd" data-category="Primary Antibodies" data-id="BAF210" data-name="Human TNF-alpha Biotinylated Antibody" data-position="0"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/antibodies/human-tnf-alpha-biotinylated-antibody_baf210" title="Human TNF-alpha Biotinylated Antibody"> <h4>Human TNF-alpha Biotinylated Antibody <span class="rounded align-middle p-1 badge best-seller" title="Best Seller">Best Seller</span> </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="R&D Systems, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-rnd-systems.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/antibodies/human-tnf-alpha-biotinylated-antibody_baf210" title="Human TNF-alpha Biotinylated Antibody"> BAF210 </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/antibodies/human-tnf-alpha-biotinylated-antibody_baf210#reviews" class="searchImage_star5"> (1) </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/antibodies/human-tnf-alpha-biotinylated-antibody_baf210#citations"><span class="icon-icon-product-citations"></span>Citations (69)</a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/antibodies/human-tnf-alpha-biotinylated-antibody_baf210#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> <div class="d-none d-md-inline my-1"> <a href="#" class="search_result_images"><span class="icon-icon-image-camera-plus"></span> Images (1)</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> WB, ICC </div> <div class="d-none d-md-block"> <span class="species_title"><strong>Reactivity:</strong></span> Human </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-0" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-0"> <div class="row"> <div class="search_result_description col-xs-12 col-md-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Reactivity:</strong></td> <td class="pb-1 w-75 text-break"> Human </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Details:</strong></td> <td class="pb-1 w-75 text-break"> Goat IgG Polyclonal </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> WB, ICC </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="baf210"></div> </div> <div class="search_result_image_wrapper col-xs-12 col-md-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="rnd"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src=https://resources.bio-techne.com/images/products/antibody/TNFalpha_BAF210_Immunocytochemistry__Immunofluorescence_19771.jpg data-srcset=https://resources.bio-techne.com/images/products/antibody/TNFalpha_BAF210_Immunocytochemistry__Immunofluorescence_19771.jpg alt="TNF-a antibody in Human PBMCs by Immunocytochemistry (ICC)." title="TNF‑ alpha in Human PBMCs." data-caption="TNF-a was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human TNF-a Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF210) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." data-badges="" data-application="Immunocytochemistry" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-BAF210" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="BAF210"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart-BAF210" class="atc-lines"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="BAF210"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> BAF210 </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> </div> <div class="atc-control ProductCart-BAF210-control text-right"> <div class="row d-flex justify-content-end mx-0 p-0"> <div class="order-md-last m___button-atc mx-1"> <button type="submit" value="Add To Cart" class="atc-add-button btn a___button-atc m-1 col-xs-4 order-last btn-md-block w-100"> Add To Cart </button> </div> <div class="m___button-atc mx-1"> <a href="/services/bulk-quotes?product_code=BAF210" class="atc-bulk-button btn a___button-secondary m-1 col-xs-4 btn-md-block w-100" rel="nofollow">Bulk Order</a> </div> </div> </div> </div> </form> </div> </div> </div> </div> <div class="search_result atc-container pb-3" data-brand="rnd" data-category="Luminex Assays" data-id="FCSTM09" data-name="Human High Sensitivity Cytokine A Premixed Magnetic Luminex® Performance Assay" data-position="20"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/luminex-assays/human-high-sensitivity-cytokine-a-premixed-magnetic-luminex-performance-assay_fcstm09" title="Human High Sensitivity Cytokine A Premixed Magnetic Luminex® Performance Assay"> <h4>Human High Sensitivity Cytokine A Premixed Magnetic Luminex® Performance Assay <span class="rounded align-middle p-1 badge best-seller" title="Best Seller">Best Seller</span> </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="R&D Systems, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-rnd-systems.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/luminex-assays/human-high-sensitivity-cytokine-a-premixed-magnetic-luminex-performance-assay_fcstm09" title="Human High Sensitivity Cytokine A Premixed Magnetic Luminex® Performance Assay"> FCSTM09 </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/luminex-assays/human-high-sensitivity-cytokine-a-premixed-magnetic-luminex-performance-assay_fcstm09#reviews" class="searchImage_star5"> (2) </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/luminex-assays/human-high-sensitivity-cytokine-a-premixed-magnetic-luminex-performance-assay_fcstm09#citations"><span class="icon-icon-product-citations"></span>Citations (29)</a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/luminex-assays/human-high-sensitivity-cytokine-a-premixed-magnetic-luminex-performance-assay_fcstm09#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> <div class="d-none d-md-inline my-1"> <a href="#" class="search_result_images"><span class="icon-icon-image-camera-plus"></span> Images (2)</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> ELISA </div> <div class="d-none d-md-block"> </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-20" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-20"> <div class="row"> <div class="search_result_description col-xs-12 col-md-9"> <p>Luminex High Performance Assays go through rigorous development, validation, and quality control testing to ensure you are getting the best performance out of your multiplex assay. Panels are customizable so you can order a selection of the analytes or the entire panel.</p> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> ELISA </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="fcstm09"></div> </div> <div class="search_result_image_wrapper col-xs-12 col-md-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="rnd"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src=https://resources.bio-techne.com/images/products/fcstm09_human-hs-cytokine-a-premixed-mag-luminex-performance-assay-18112020132516.png data-srcset=https://resources.bio-techne.com/images/products/fcstm09_human-hs-cytokine-a-premixed-mag-luminex-performance-assay-18112020132516.png alt="Human Luminex High Sensitivity Cytokine A Panel Standard Curves" title="Human Luminex High Sensitivity Cytokine A Panel Standard Curves" data-caption="" data-badges="" data-application="Multiplex Immunoassay" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> <span class="image-count-text align-middle">(2)</span> </div> </div> </div> <i data-src="https://resources.bio-techne.com/images/products/fcstm09_human-hs-cytokine-a-premixed-mag-luminex-performance-assay-2872020162821.jpg" data-alt="Human Luminex High Sensitivity Cytokine A Premixed Magnetic Performance Assay" title="Human Luminex High Sensitivity Cytokine A Premixed Magnetic Performance Assay" data-caption="" data-badges="" data-application="Multiplex Immunoassay" data-width="250" data-height="250" class="none"></i> </div> </div> </div> </div> <div class="mt-2 text-right"> </div> </div> </div> </div> <div class="search_result atc-container pb-3" data-brand="rnd" data-category="Proteins and Enzymes" data-id="210-GMP" data-name="Recombinant Human TNF-alpha GMP Protein, CF" data-position="24"> <div class="search_details border bg-white p-3 rounded"> <div class="row"> <div class="col-md-9"> <a href="/p/proteins-enzymes/recombinant-human-tnf-alpha-gmp-protein-cf_210-gmp" title="Recombinant Human TNF-alpha GMP Protein, CF"> <h4>Recombinant Human TNF-alpha GMP Protein, CF <span class="rounded align-middle p-1 badge best-seller" title="Best Seller">Best Seller</span> <span class="a___badge-gmp badge text-white align-middle p-1" title="GMP Product">GMP</span> </h4> </a> </div> <div class="search_result_brand_logo col-md-3 mb-1 d-flex justify-content-xl-end product-brand-logo"> <img alt="R&D Systems, a Bio-Techne brand" src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-rnd-systems.svg" class="d-flex w-75"> </div> </div> <div class="row"> <div class="col-md-9"> <div class="mb-1 d-inline-flex"> <div class="align-middle my-1 mr-3 d-block d-md-inline"> Catalog #: <a href="/p/proteins-enzymes/recombinant-human-tnf-alpha-gmp-protein-cf_210-gmp" title="Recombinant Human TNF-alpha GMP Protein, CF"> 210-GMP </a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold"> <a href="/p/proteins-enzymes/recombinant-human-tnf-alpha-gmp-protein-cf_210-gmp#citations"><span class="icon-icon-product-citations"></span>Citations (5)</a> </div> <div class="align-middle my-1 mr-3 d-block d-md-inline font-weight-bold text-nowrap"> <a href="/p/proteins-enzymes/recombinant-human-tnf-alpha-gmp-protein-cf_210-gmp#product-documents"><span class="icon-icon-pdf text-error-red"></span> Product Documents</a> </div> <div class="d-none d-md-inline my-1"> <a href="#" class="search_result_images"><span class="icon-icon-image-camera-plus"></span> Images (3)</a> </div> </div> <div class="d-none d-md-block"> <span class="search_result_applications"></span><span class="applications_title"><strong>Applications:</strong></span> BA </div> <div class="d-none d-md-block"> </div> </div> <div class="col-md-3"> <button class="py-1 btn-default btn-block atc-view-more collapsed" data-toggle="collapse" href="#collapseButton-24" role="button" aria-expanded="false" aria-controls="collapseButton">View More</button> </div> </div> <div class="row"> <div class="align-middle mt-2 col-xs-12 col-sm-9"> <p class="mb-0">Animal-Free.</p> </div> </div> <div class="commerce-atc-search pt-3 collapse" id="collapseButton-24"> <div class="row"> <div class="search_result_description col-xs-12 col-md-9"> <table class="tablesaw tablesaw-stack mb-2"> <tbody> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Source:</strong></td> <td class="pb-1 w-75 text-break"> E. coli </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Accession #:</strong></td> <td class="pb-1 w-75 text-break"> P01375.1 </td> </tr> <tr class="search_result_product_detail"> <td class="pb-1 align-top text-md-right"><strong>Applications:</strong></td> <td class="pb-1 w-75 text-break"> BA </td> </tr> </tbody> </table> <div class="compare_tool_select form-inline mb-2" data-sku="210-gmp"></div> </div> <div class="search_result_image_wrapper col-xs-12 col-md-3"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="rnd"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src=https://resources.bio-techne.com/images/products/protein/TNF-alpha_210-GMP_32.jpg data-srcset=https://resources.bio-techne.com/images/products/protein/TNF-alpha_210-GMP_32.jpg alt="Recombinant Human TNF-alpha GMP Protein Bioactivity" title="Recombinant Human TNF-alpha GMP Protein Bioactivity" data-caption="GMP-grade Recombinant Human TNF-alpha (Catalog # 210-GMP) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED<sub>50</sub> is 25-100 pg/mL." data-badges="" data-application="Bioactivity" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> <span class="image-count-text align-middle">(3)</span> </div> </div> </div> <i data-src="https://resources.bio-techne.com/images/products/protein/TNF-alpha_210-GMP_30.jpg" data-alt="Recombinant Human TNF-alpha GMP Protein SDS-PAGE" title="Recombinant Human TNF-alpha GMP Protein SDS-PAGE" data-caption="1 μg/lane of GMP-grade Recombinant Human TNF-alpha (Catalog # 210-GMP) was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a single band at 17 kDa." data-badges="" data-application="SDS-Page" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/protein/TNFalpha_210GMP_858.jpg" data-alt="Recombinant Human TNF-alpha GMP Protein Mass Spectrometry" title="Recombinant Human TNF-alpha GMP Protein Mass Spectrometry" data-caption="MALDI-TOF analysis of GMP-grade Recombinant Human TNF-alpha (Catalog # 210‑GMP). The major peak at 17348 corresponds to the calculated molecular mass without an N-terminal Met, 17353 Da. The minor peak at 17480 corresponds to the calculated molecular mass with an N-terminal Met, 17484 Da. The peak at 17565 is a matrix-associated artifact of the MALDI-TOF." data-badges="" data-application="Mass Spectrometry" data-width="250" data-height="250" class="none"></i> </div> </div> </div> </div> <div class="atc-block-search atc-block-collapsible" data-type="search" data-blockid="ProductCart-210-GMP" > <form action="/commerce/addtocart" method="post" class="px-3 pt-3 add-to-cart-block" data-productcode="210-GMP"> <div class="commerce-atc-full-header atc-header col-four row border-bottom pb-1 m-0"> <div class="commerce-atc-catnum atc-head col-4 col-md-4 font-weight-bold">Catalog #</div> <div class="commerce-atc-availability atc-head col-md-3 font-weight-bold d-none d-md-block">Availability </div> <div class="commerce-atc-price atc-head col-5 col-md-3 font-weight-bold">Size / Price</div> <div class="commerce-atc-quantity atc-head col-3 col-md-2 text-right font-weight-bold">Qty</div> </div> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> </div> <div class="d-none search-product-mask"> <div id="ProductCart-210-GMP" class="atc-lines"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="210-GMP-02M"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> 210-GMP-02M </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> <div class="commerce-atc-search-productline productline col-four row py-2 m-0" data-catalog="210-GMP-100"> <div class="commerce-atc-catnum col-4 col-md-4 my-2"> 210-GMP-100 </div> <div class="commerce-atc-availability col-md-3 my-2 d-none d-md-block"> </div> <div class="commerce-atc-price col-5 col-md-3 my-2"> </div> <div class="commerce-atc-quantity col-3 col-md-2 my-2 text-right"> </div> </div> <hr class="d-md-none bg-light"> 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