CINXE.COM

<!DOCTYPE html> <html lang="de-DE"> <head><script type="text/javascript" src="/_static/js/bundle-playback.js?v=HxkREWBo" charset="utf-8"></script> <script type="text/javascript" src="/_static/js/wombat.js?v=txqj7nKC" charset="utf-8"></script> <script>window.RufflePlayer=window.RufflePlayer||{};window.RufflePlayer.config={"autoplay":"on","unmuteOverlay":"hidden"};</script> <script type="text/javascript" src="/_static/js/ruffle/ruffle.js"></script> <script type="text/javascript"> __wm.init("https://web.archive.org/web"); __wm.wombat("https://tron-mainz.de/de/2018-2/","20210320060823","https://web.archive.org/","web","/_static/", "1616220503"); </script> <link rel="stylesheet" type="text/css" href="/_static/css/banner-styles.css?v=S1zqJCYt" /> <link rel="stylesheet" type="text/css" href="/_static/css/iconochive.css?v=3PDvdIFv" />  <meta charset="UTF-8"/> <title>2018 | TRON Translational Oncology Mainz | Connecting Competence</title> <meta http-equiv="Content-Type" content="text/html; charset=UTF-8"/> <link rel="pingback" href="https://tron-mainz.de/de/xmlrpc.php"/> <script>window.koko_analytics = {"tracker_url":"https:\/\/web.archive.org\/web\/20210320060823\/https:\/\/tron-mainz.de\/de\/wp-admin\/admin-ajax.php?action=koko_analytics_collect","post_id":8451,"use_cookie":1,"cookie_path":"\/de","honor_dnt":true};</script><script src="https://web.archive.org/web/20210320060823js_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/koko_analytics/assets/dist/js/script_js.js" async="async"></script>  <meta content="initial-scale=1.0, maximum-scale=1.0, user-scalable=yes" name="viewport"/> <style type="text/css"> @media only screen and (min-width: 980px) { body.one-col-1200 #main-sidebar-container { width: 100%; } body.one-col-1200 #main-sidebar-container #main { width: 100%; } } </style> <link rel="stylesheet" id="nb-core-css" href="https://web.archive.org/web/20210320060823cs_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/swift_box_wp/js/sb/swift_box_layout_min_css.css" type="text/css" media="all"/> <link rel="stylesheet" id="ctf_styles-css" href="https://web.archive.org/web/20210320060823cs_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/custom_twitter_feeds/css/ctf_styles_min_css.css" type="text/css" media="all"/> <link rel="stylesheet" id="default-icon-styles-css" href="https://web.archive.org/web/20210320060823cs_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/svg_vector_icon_plugin/public/_/admin/css/wordpress_svg_icon_plugin_style_min_css.css" type="text/css" media="all"/> <link rel="stylesheet" id="SFSIPLUSmainCss-css" href="https://web.archive.org/web/20210320060823cs_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/ultimate_social_media_plus/css/sfsi_style_css.css" type="text/css" media="all"/> <link rel="stylesheet" id="disable_sfsiplus-css" href="https://web.archive.org/web/20210320060823cs_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/ultimate_social_media_plus/css/disable_sfsi_css.css" type="text/css" media="all"/> <link rel="stylesheet" id="wpmt-css-frontend-css" href="https://web.archive.org/web/20210320060823cs_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/wp_mailto_links/core/includes/assets/css/style_css.css" type="text/css" media="all"/> <link rel="stylesheet" id="tablepress-default-css" href="https://web.archive.org/web/20210320060823cs_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/tablepress/css/default_min_css.css" type="text/css" media="all"/> <link rel="stylesheet" id="mlp_frontend_css-css" href="https://web.archive.org/web/20210320060823cs_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/multilingual_press/assets/css/frontend_min_css.css" type="text/css" media="all"/> <link rel="stylesheet" id="js_composer_front-css" href="https://web.archive.org/web/20210320060823cs_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/uploads/sites/9/js_composer/js_composer_front_custom_css.css" type="text/css" media="all"/> <link rel="stylesheet" id="rightalign-css" href="https://web.archive.org/web/20210320060823cs_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/cx_menu_customizer/styles/rightalign_css.css" type="text/css" media="all"/> <link rel="stylesheet" id="theme-stylesheet-css" href="https://web.archive.org/web/20210320060823cs_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/themes/canvas/style_css.css" type="text/css" media="all"/> <link rel="stylesheet" id="woo-gravity-forms-css" href="https://web.archive.org/web/20210320060823cs_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/themes/canvas/includes/integrations/gravity_forms/css/gravity_forms_css.css" type="text/css" media="all"/> <link rel="stylesheet" id="mpc-massive-main-style-css" href="https://web.archive.org/web/20210320060823cs_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/mpc_massive_pack_col/assets/css/mpc_main_min_css.css" type="text/css" media="all"/> <link rel="stylesheet" id="pnm-sticky-nav-css-css" href="https://web.archive.org/web/20210320060823cs_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/cx_menu_customizer/styles/sticky_nav_css.css" type="text/css" media="all"/> <link rel="stylesheet" id="pnm-top-nav-css" href="https://web.archive.org/web/20210320060823cs_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/cx_menu_customizer/styles/top_nav_css.css" type="text/css" media="all"/> <style id="pnm-top-nav-inline-css" type="text/css"> /* Top Nav CSS */ @media only screen and ( min-width: 768px ) { /* Top Nav Customizer Styles */ #top .col-full { text-align : right; padding-top : px; padding-bottom : px; } #top .col-full #top-nav > .menu-item { border-right: 0.5px solid #ffffff; } #top .col-full #top-nav > .menu-item > a, #top ul > li > a { font-family: Open Sans ; font-size: 0.9em ; font-style: normal ; font-weight: 700; color : #ffffff !important; } #top .col-full #top-nav li.current_page_item a, #top .col-full #top-nav li.current_page_parent a, #top .col-full #top-nav li.current-menu-ancestor a, #top .col-full #top-nav li.current-cat a, #top .col-full #top-nav li.current-menu-item a { color: #164679 !important; background: transparent !important; } div#top {background : #164679; } #top .col-full #top-nav > li:hover , #top #top-nav > li:hover > a , #top ul ul {background : #be3026 !important; } #top .col-full #top-nav > li:hover * {color : #ffffff !important; } #top .col-full #top-nav li.current_page_item, #top .col-full #top-nav li.current_page_parent, #top .col-full #top-nav li.current-menu-ancestor, #top .col-full #top-nav li.current-cat, #top .col-full #top-nav li.current-menu-item {background-color : !important; } #top .col-full #top-nav li.current_page_item a, #top .col-full #top-nav li.current_page_parent a, #top .col-full #top-nav li.current-menu-ancestor a, #top .col-full #top-nav li.current-cat a, #top .col-full #top-nav li.current-menu-item a {color : #be3026 !important; } #top .col-full ul li a{ line-height : 1.6 !important; } #top .col-full #top-nav > .menu-item:first-child { border-left: 0.5px solid #ffffff; } /* Top Nav Icons */ #top ul.nav > li.menu-item > a > i, #top .col-full ul > li > a { color : !important; } #top ul.nav > li > a:hover > i { color : ; } #top ul.nav > li.current-menu-item > a > i { color : ; } #top ul.sub-menu > li > a > i { color : ; } } </style>  <script type="text/javascript" src="https://web.archive.org/web/20210320060823js_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_includes/js/jquery/jquery_js.js" id="jquery-core-js"></script> <script type="text/javascript" id="pnm-front-end-script-js-extra"> /* <![CDATA[ */ var PNMMenuItem = {"bgColor":"#ffffff","currentPageBgColor":"#ffffff","hoverBgColor":"","ctaMenuItemID":"","ctaMenuItemBgColor":"","ctaHoverMenuItemBgColor":"","ctaCurrentMenuItemBgColor":"","isSubMenuCenteredUnderMain":"","subMenuArrow":"No arrow","arrowHeight":""}; var PNMAlign = {"alignMenuRight":"1"}; /* ]]> */ </script> <script type="text/javascript" src="https://web.archive.org/web/20210320060823js_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/cx_menu_customizer/scripts/front_end_script_js.js" id="pnm-front-end-script-js"></script> <script type="text/javascript" src="https://web.archive.org/web/20210320060823js_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/cx_menu_customizer/scripts/imagesloaded_pkgd_min_js.js" id="pnm-images-loaded-script-js"></script> <script type="text/javascript" src="https://web.archive.org/web/20210320060823js_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_includes/js/jquery/jquery_migrate_min_js.js" id="jquery-migrate-js"></script> <script type="text/javascript" src="https://web.archive.org/web/20210320060823js_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/wp_mailto_links/core/includes/assets/js/custom_js.js" id="wpmt-js-frontend-js"></script> <script type="text/javascript" src="https://web.archive.org/web/20210320060823js_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/cx_menu_customizer/scripts/align_menu_right_js.js" id="align-menu-right-js"></script> <script type="text/javascript" src="https://web.archive.org/web/20210320060823js_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/cx_menu_customizer/scripts/sticky_nav_js.js" id="pnm-sticky-nav-js-js"></script> <script type="text/javascript" src="https://web.archive.org/web/20210320060823js_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/themes/canvas/includes/js/third_party_min_js.js" id="third-party-js"></script> <script type="text/javascript" src="https://web.archive.org/web/20210320060823js_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/themes/canvas/includes/js/modernizr_min_js.js" id="modernizr-js"></script> <script type="text/javascript" src="https://web.archive.org/web/20210320060823js_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/themes/canvas/includes/js/general_min_js.js" id="general-js"></script>  <style type="text/css"> .col-full, #wrapper { max-width: 1200px !important; } </style> <link rel="https://api.w.org/" href="https://tron-mainz.de/de/wp-json/"/><link rel="alternate" type="application/json" href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/wp-json/wp/v2/pages/8451"/><meta name="generator" content="WordPress 5.5.3"/> <link rel="canonical" href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/2018-2/"/> <link rel="shortlink" href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/?p=8451"/> <link rel="alternate" type="application/json+oembed" href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/wp-json/oembed/1.0/embed?url=https%3A%2F%2Ftron-mainz.de%2Fde%2F2018-2%2F"/> <link rel="alternate" type="text/xml+oembed" href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/wp-json/oembed/1.0/embed?url=https%3A%2F%2Ftron-mainz.de%2Fde%2F2018-2%2F&format=xml"/>  <style type="text/css"> @media only screen and (max-width: 600px) { .page .lcnb_news_inner { display: -webkit-box; display: -ms-flexbox; display: flex; -webkit-box-orient: vertical; -webkit-box-direction: normal; -ms-flex-direction: column; flex-direction: column; } .page .lcnb_vertical .lcnb_img>div img { width: 80%; max-width: 80%; height: auto!important; margin-left: -40px!important; margin-top:20px!important; } .lcnb_wpdt_theme.lcnb_wrap.lcnb_boxed article.lcnb_news {height: auto!important;} .lcnb_news.lcnb_type_inline {height: auto!important;} }</style>   <style type="text/css"> /* Add your CSS code here. For example: .example { color: red; } For brushing up on your CSS knowledge, check out http://www.w3schools.com/css/css_syntax.asp End of comment */ .gform_wrapper div.validation_error {border:none;} .gform_wrapper li.gfield.gfield_error {background:none;}</style>   <style type="text/css"> input.wpcf7-form-control.wpcf7-submit { background-color: #164679; border-radius: 0; font-weight: 400; } input.wpcf7-form-control.wpcf7-text.wpcf7-email { border-radius: 0; }</style>   <style type="text/css"> .post-meta {display: none;} .post-6679 .title {display:none;}</style>   <style type="text/css"> @media screen and (min-width: 768px) { #logo { max-width: 18%; } } @media screen and (max-width: 768px) { #logo { max-width: 30%; } }</style>   <style type="text/css"> .footer-menu a {color: #ffffff;} .footer-menu a {text-decoration: none;} .footer-menu a:hover {color: #be3026; text-decoration: none;}</style>   <style type="text/css"> /* Traditional VC Grid */ .homegrid { text-transform: uppercase; } .vc_gitem-row-position-bottom { right: auto!important; } .vc_gitem-zone .vc-zone-link { color: #fff!important; } /* Extra Fancy VC Grid* /*Blend in fully on hover .mpc-post .mpc-post__thumbnail { opacity: 0.5; } .mpc-post .mpc-post__thumbnail:hover { opacity: 1; } .mpc-post__heading :hover { color: #ffffff; } */ /*No Load More*/ .mpc-pagination { display: none!important; } /*distance 1st and 2nd row*/ div.wpb_content_element { margin-bottom: 0px!important; }</style>   <link href="https://web.archive.org/web/20210320060823cs_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/fonts.googleapis.com/css_family_Lato_3A100_2C878949587.css" rel="stylesheet" type="text/css"/> <script> window.addEventListener("sfsi_plus_functions_loaded", function() { var body = document.getElementsByTagName('body')[0]; // console.log(body); body.classList.add("sfsi_plus_3.47"); }) // window.addEventListener('sfsi_plus_functions_loaded',function(e) { // jQuery("body").addClass("sfsi_plus_3.47") // }); jQuery(document).ready(function(e) { jQuery("body").addClass("sfsi_plus_3.47") }); function sfsi_plus_processfurther(ref) { var feed_id = 'YjBieUhIbjlSaTlwVGJ3TCttMnFRWTNDY2NxN1pUUlpTN2hkZm9zZ1BSTHdJTE1NVFZmU2I5MkF4Z1RPK3FqN0lNR2Vjd1l3NVE3eC9hVzJCR092MUhxNVY3ek52SlpyQ2kvdm9MUXViT0FYdSs1eW1YeWtYdEg3RFRqNm1lMEV8bzBEc0VDNjlTMzdxb3FrOUdNYzJrbmxEbEtaQlI5WTNGQTZIc3VQQVo5ND0='; var feedtype = 8; var email = jQuery(ref).find('input[name="email"]').val(); var filter = /^([a-zA-Z0-9_\.\-])+\@(([a-zA-Z0-9\-])+\.)+([a-zA-Z0-9]{2,4})+$/; if ((email != "Enter your email") && (filter.test(email))) { if (feedtype == "8") { var url = "https://web.archive.org/web/20210320060823/https://api.follow.it/subscription-form/" + feed_id + "/" + feedtype; window.open(url, "popupwindow", "scrollbars=yes,width=1080,height=760"); return true; } } else { alert("Please enter email address"); jQuery(ref).find('input[name="email"]').focus(); return false; } } </script> <style> .sfsi_plus_subscribe_Popinner { width: 100% !important; height: auto !important; border: 1px solid #b5b5b5 !important; padding: 18px 0px !important; background-color: #ffffff !important; } .sfsi_plus_subscribe_Popinner form { margin: 0 20px !important; } .sfsi_plus_subscribe_Popinner h5 { font-family: Helvetica,Arial,sans-serif !important; font-weight: bold !important; color: #000000 !important; font-size: 16px !important; text-align: center !important; margin: 0 0 10px !important; padding: 0 !important; } .sfsi_plus_subscription_form_field { margin: 5px 0 !important; width: 100% !important; display: inline-flex; display: -webkit-inline-flex; } .sfsi_plus_subscription_form_field input { width: 100% !important; padding: 10px 0px !important; } .sfsi_plus_subscribe_Popinner input[type=email] { font-family: Helvetica,Arial,sans-serif !important; font-style: normal !important; color: !important; font-size: 14px !important; text-align: center !important; } .sfsi_plus_subscribe_Popinner input[type=email]::-webkit-input-placeholder { font-family: Helvetica,Arial,sans-serif !important; font-style: normal !important; color: !important; font-size: 14px !important; text-align: center !important; } .sfsi_plus_subscribe_Popinner input[type=email]:-moz-placeholder { /* Firefox 18- */ font-family: Helvetica,Arial,sans-serif !important; font-style: normal !important; color: !important; font-size: 14px !important; text-align: center !important; } .sfsi_plus_subscribe_Popinner input[type=email]::-moz-placeholder { /* Firefox 19+ */ font-family: Helvetica,Arial,sans-serif !important; font-style: normal !important; color: !important; font-size: 14px !important; text-align: center !important; } .sfsi_plus_subscribe_Popinner input[type=email]:-ms-input-placeholder { font-family: Helvetica,Arial,sans-serif !important; font-style: normal !important; color: !important; font-size: 14px !important; text-align: center !important; } .sfsi_plus_subscribe_Popinner input[type=submit] { font-family: Helvetica,Arial,sans-serif !important; font-weight: bold !important; color: #000000 !important; font-size: 16px !important; text-align: center !important; background-color: #dedede !important; } </style> <meta name="follow.it-verification-code-YjBieUhIbjlSaTlwVGJ3TCttMnFRWTNDY2NxN1pUUlpTN2hkZm9zZ1BSTHdJTE1NVFZmU2I5MkF4Z1RPK3FqN0lNR2Vjd1l3NVE3eC9hVzJCR092MUhxNVY3ek52SlpyQ2kvdm9MUXViT0FYdSs1eW1YeWtYdEg3RFRqNm1lMEV8bzBEc0VDNjlTMzdxb3FrOUdNYzJrbmxEbEtaQlI5WTNGQTZIc3VQQVo5ND0=" content="d4BS8a1CMehBt5r4ASFd"/> <meta name="viewport" content="width=device-width, initial-scale=1"><meta property="og:image:secure_url" content="https://tron-mainz.de/de/wp-content/uploads/sites/9/2018/01/TRONPublications.jpg" data-id="sfsi-plus"/><meta property="og:image:type" content="" data-id="sfsi-plus"/><meta property="og:image:width" content="640" data-id="sfsi-plus"/><meta property="og:image:height" content="427" data-id="sfsi-plus"/><meta property="og:description" content="[vc_row gap=15][vc_column width=3/4][vc_column_text] Vormehr M, Reinhard K, Blatnik R, Josef K, Beck JD, Salomon N, Suchan M, Selmi A, Vascotto F, Zerweck J, Wenschuh H, Diken M, Kreiter S, Türeci Ö, Riemer AB, Sahin U. (2018) A non-functional neoepitope specific CD8+ T-cell response induced by tumor derived antigen exposure in vivo. Oncoimmunology. 8(3):1553478. DOI; PMID [su_spoiler title=Show abstract] Cancer-associated mutations, mostly single nucleotide variations, can act as neoepitopes and prime targets for effective anti-cancer T-cell immunity. T cells recognizing cancer mutations are critical for the clinical activity of immune checkpoint blockade (ICB) and they are potent vaccine antigens. High frequencies of mutation-specific T cells are rarely spontaneously induced. Hence, therapies that broaden the tumor specific T-cell response are of interest. Here, we analyzed neoepitope-specific CD8+ T-cell responses mounted either spontaneously or after immunotherapy regimens, which induce local tumor inflammation and cell death, in mice bearing tumors of the widely used colon carcinoma cell line CT26. A comprehensive immune reactivity screening of 2474 peptides covering 628 transcribed CT26 point mutations was conducted. All tested treatment regimens were found to induce a single significant CD8+ T-cell response against a non-synonymous D733A point mutation in the Smc3 gene. Surprisingly, even though Smc3 D733A turned out to be the immune-dominant neoepitope in CT26 tumor bearing mice, neither T cells specific for this neoepitope nor their T cell receptors (TCRs) were able to recognize or lyse tumor cells. Moreover, vaccination with the D733A neoepitope did not result in anti-tumoral activity despite induction of specific T cells. This is to our knowledge the first report that neoepitope specific CD8+ T cells primed by tumor-released antigen exposure in vivo can be functionally irrelevant. [/su_spoiler] Hilf N, Kuttruff-Coqui S, Frenzel K, Bukur V, Stevanović S, Gouttefangeas C, Platten M, Tabatabai G, Dutoit V, van der Burg SH, Thor Straten P, Martínez-Ricarte F, Ponsati B, Okada H, Lassen U, Admon A, Ottensmeier CH, Ulges A, Kreiter S, von Deimling A, Skardelly M, Migliorini D, Kroep JR, Idorn M, Rodon J, Piró J, Poulsen HS, Shraibman B, McCann K, Mendrzyk R, Löwer M, Stieglbauer M, Britten CM, Capper D, Welters MJP, Sahuquillo J, Kiesel K, Derhovanessian E, Rusch E, Bunse L, Song C, Heesch S, Wagner C, Kemmer-Brück A, Ludwig J, Castle JC, Schoor O, Tadmor AD, Green E, Fritsche J, Meyer M, Pawlowski N, Dorner S, Hoffgaard F, Rössler B, Maurer D, Weinschenk T, Reinhardt C, Huber C, Rammensee HG, Singh-Jasuja H, Sahin U, Dietrich PY, Wick W. (2018) Actively personalized vaccination trial for newly diagnosed glioblastoma. Nature. 565(7738):240-245. DOI; PMID [su_spoiler title=Show abstract] Patients with glioblastoma currently do not sufficiently benefit from recent breakthroughs in cancer treatment that use checkpoint inhibitors1,2. For treatments using checkpoint inhibitors to be successful, a high mutational load and responses to neoepitopes are thought to be essential3. There is limited intratumoural infiltration of immune cells4 in glioblastoma and these tumours contain only 30–50 non-synonymous mutations5. Exploitation of the full repertoire of tumour antigens—that is, both unmutated antigens and neoepitopes—may offer more effective immunotherapies, especially for tumours with a low mutational load. Here, in the phase I trial GAPVAC-101 of the Glioma Actively Personalized Vaccine Consortium (GAPVAC), we integrated highly individualized vaccinations with both types of tumour antigens into standard care to optimally exploit the limited target space for patients with newly diagnosed glioblastoma. Fifteen patients with glioblastomas positive for human leukocyte antigen (HLA)-A*02:01 or HLA-A*24:02 were treated with a vaccine (APVAC1) derived from a premanufactured library of unmutated antigens followed by treatment with APVAC2, which preferentially targeted neoepitopes. Personalization was based on mutations and analyses of the transcriptomes and immunopeptidomes of the individual tumours. The GAPVAC approach was feasible and vaccines that had poly-ICLC (polyriboinosinic-polyribocytidylic acid-poly-L-lysine carboxymethylcellulose) and granulocyte–macrophage colony-stimulating factor as adjuvants displayed favourable safety and strong immunogenicity. Unmutated APVAC1 antigens elicited sustained responses of central memory CD8+ T cells. APVAC2 induced predominantly CD4+ T cell responses of T helper 1 type against predicted neoepitopes. [/su_spoiler] Fiore A, Ugel S, Sanctis FD, Sandri S, Fracasso G, Trovato R, Sartoris S, Solito S, Mandruzzato S, Vascotto F, Hippen KL, Mondanelli G, Grohmann U, Piro G, Carbone C, Melisi D, Lawlor RT, Scarpa A, Lamolinara A, Iezzi M, Fassan M, Bicciato S, Blazar BR, Sahin U, Murray PJ, Bronte V. (2018) Induction of immunosuppressive functions and NF-kB by FLIP in monocytes. Nat Commun. 9:5193. DOI; PMID [su_spoiler title=Show abstract] Immunosuppression is a hallmark of tumor progression, and treatments that inhibit or deplete monocytic myeloid-derived suppressive cells could promote anti-tumor immunity. c-FLIP is a central regulator of caspase-8-mediated apoptosis and necroptosis. Here we show that low-dose cytotoxic chemotherapy agents cause apoptosis linked to c-FLIP down-regulation selectively in monocytes. Enforced expression of c-FLIP or viral FLIP rescues monocytes from cytotoxicity and concurrently induces potent immunosuppressive activity, in T cell cultures and in vivo models of tumor progression and immunotherapy. FLIP-transduced human blood monocytes can suppress graft versus host disease. Neither expression of FLIP in granulocytes nor expression of other anti-apoptotic genes in monocytes conferred immunosuppression, suggesting that FLIP effects on immunosuppression are specific to monocytic lineage and distinct from death inhibition. Mechanistically, FLIP controls a broad transcriptional program, partially by NF-κB activation. Therefore, modulation of FLIP in monocytes offers a means to elicit or block immunosuppressive myeloid cells. [/su_spoiler] Sun Q, Barz M, De Geest BG, Diken M, Hennink WE, Kiessling F, Lammers T, Shi Y. (2018) Nanomedicine and macroscale materials in immuno-oncology. Chem Soc Rev. 48(1):351-381. DOI; PMID [su_spoiler title=Show abstract] Immunotherapy is revolutionizing the treatment of cancer. It can achieve unprecedented responses in advanced-stage patients, including complete cures and long-term survival. However, immunotherapy also has limitations, such as its relatively low response rates and the development of severe side effects. These drawbacks are gradually being overcome by improving our understanding of the immune system, as well as by establishing combination regimens in which immunotherapy is combined with other treatment modalities. In addition to this, in recent years, progress made in chemistry, nanotechnology and materials science has started to impact immuno-oncology, resulting in more effective and less toxic immunotherapy interventions. In this context, multiple different nanomedicine formulations and macroscale materials have been shown to be able to boost anti-cancer immunity and the efficacy of immunomodulatory drugs. We here review nanotechnological and materials chemistry efforts related to endogenous and exogenous vaccination, to the engineering of antigen-presenting cells and T cells, and to the modulation of the tumor microenvironment. We also discuss limitations, current trends and future directions. Together, the insights provided and the evidence obtained indicate that there is a bright future ahead for engineering nanomedicines and macroscale materials for immuno-oncology applications. [/su_spoiler] Pastor F, Berraondo P, Etxeberria I, Frederick J, Sahin U, Gilboa E and Melero I. (2018) An RNA toolbox for cancer immunotherapy. Nat Rev. Drug Discov. 17(10):751-767. DOI; PMID [su_spoiler title=Show abstract] Cancer immunotherapy has revolutionized oncology practice. However, current protein and cell therapy tools used in cancer immunotherapy are far from perfect, and there is room for improvement regarding their efficacy and safety. RNA-based structures have diverse functions, ranging from gene expression and gene regulation to pro-inflammatory effects and the ability to specifically bind different molecules. These functions make them versatile tools that may advance cancer vaccines and immunomodulation, surpassing existing approaches. These technologies should not be considered as competitors of current immunotherapies but as partners in synergistic combinations and as a clear opportunity to reach more efficient and personalized results. RNA and RNA derivatives can be exploited therapeutically as a platform to encode protein sequences, provide innate pro-inflammatory signals to the immune system (such as those denoting viral infection), control the expression of other RNAs (including key immunosuppressive factors) post-transcriptionally and conform structural scaffoldings binding proteins that control immune cells by modifying their function. Nascent RNA immunotherapeutics include RNA vaccines encoding cancer neoantigens, mRNAs encoding immunomodulatory factors, viral RNA analogues, interference RNAs and protein-binding RNA aptamers. These approaches are already in early clinical development with promising safety and efficacy results. [/su_spoiler] Pektor S, Hilscher L, Walzer KC, Miederer I, Bausbacher N, Loquai C, Schreckenberger M, Sahin U, Diken M, Miederer M. (2018) In vivo imaging of the immune response upon systemic RNA cancer vaccination by FDG-PET. EJNMMI Res. 8(1):80. DOI; PMID [su_spoiler title=Show abstract] BACKGROUND: [18F]Fluoro-2-deoxy-2-d-glucose positron emission tomography (FDG-PET) is commonly used in the clinic for diagnosis of cancer and for follow-up of therapy outcome. Additional to the well-established value in tumor imaging, it bears potential to depict immune processes in modern immunotherapies. T cells enhance their glucose consumption upon activation and are crucial effectors for the success of such novel therapies. In this study, we analyzed the T cell immunity in spleen after antigen-specific stimulation of T cells via highly innovative RNA-based vaccines using FDG-PET/MRI. For this purpose, we employed systemic administration of RNA-lipoplexes encoding the endogenous antigen of Moloney murine leukemia virus (gp70) which have been previously shown to induce potent innate as well as adaptive immune mechanisms for cancer immunotherapy. Feasibility of clinical imaging of increased splenic FDG uptake was demonstrated in a melanoma patient participating in a clinical phase 1 trial of a tetravalent RNA-lipoplex cancer vaccine. RESULTS: We observed exclusive increase of glucose uptake in spleen compared to other organs thanks to liposome-mediated RNA targeting to this immune-relevant organ. In vivo and ex vivo FDG uptake analysis in the spleen of vaccinated mice correlated well with antigen-specific T cell activation. Moreover, the use of an irrelevant (antigen non-specific) RNA also resulted in enhanced FDG uptake early after vaccination through the activation of several other splenic cell populations. The glucose uptake was also dependent on the dose of RNA administered in line with the activation and frequencies of proliferating antigen-specific T cells as well as the general activation pattern of splenic cell populations. CONCLUSIONS: Our preclinical results show rapid and transient vaccination-induced increase of FDG uptake within the spleen reflecting immune activation preceding T cell proliferation. FDG-PET/CT in patients is also capable to image this immune activation resulting in a new potential application of FDG-PET/CT to image immune processes in new immunological therapies.[/su_spoiler] Türeci Ö, Löwer M, Schrörs B, Lang M, Tadmor A, Sahin U. (2018) Challenges towards the realization of individualized cancer vaccines. Nat Biomed Eng. July 30;19:747. DOI [su_spoiler title=Show abstract] Bringing truly personalized cancer vaccination with tumour neoantigens to the clinic will require overcoming the challenges of optimized vaccine design, manufacturing and affordability, and identification of the most suitable clinical setting. [/su_spoiler] Boegel S, Bukur T, Castle JC, Sahin U (2018) In Silico Typing of Classical and Non-classical HLA Alleles from Standard RNA-Seq Reads Methods Mol Biol. 2018;1802:177-191. DOI; PMID [su_spoiler title=Show abstract] Next-Generation Sequencing (NGS) enables the rapid generation of billions of short nucleic acid sequence fragments (i.e., sequencing reads). Especially, the adoption of gene expression profiling using whole transcriptome sequencing (i.e., RNA-Seq) has been rapid. Here, we describe an in silico method, seq2HLA, that takes standard RNA-Seq reads as input and determines a sample's (classical and non-classical) HLA class I and class II types as well as HLA expression. We demonstrate the application of seq2HLA using publicly available RNA-Seq data from the Burkitt's lymphoma cell line DAUDI and the choriocarcinoma cell line JEG-3. [/su_spoiler] Schumacher J, Bacic T, Staritzbichler R, Daneschdar M, Klamp T, Arnold P, Jägle S, Türeci Ö, Markl J, Sahin U (2018) Enhanced stability of a chimeric hepatitis B core antigen virus-like-particle (HBcAg-VLP) by a C-terminal linker-hexahistidine-peptide. J Nanobiotechnology. Apr 13;16(1):39. DOI; PMID [su_spoiler title=Show abstract] BACKGROUND: Virus-like-particles (VLPs) are attractive nanoparticulate scaffolds for broad applications in material/biological sciences and medicine. Prior their functionalization, specific adaptations have to be carried out. These adjustments frequently lead to disordered particles, but the particle integrity is an essential factor for the VLP suitability. Therefore, major requirements for particle stabilization exist. The objective of this study was to evaluate novel stabilizing elements for functionalized chimeric hepatitis B virus core antigen virus-like particles (HBcAg-VLP), with beneficial characteristics for vaccine development, imaging or delivery. RESULTS: The effects of a carboxy-terminal polyhistidine-peptide and an intradimer disulfide-bridge on the stability of preclinically approved chimeric HBcAg-VLPs were assessed. We purified recombinant chimeric HBcAg-VLPs bearing different modified C-termini and compared their physical and chemical particle stability by quantitative protein-biochemical and biophysical techniques. We observed lower chemical resistance of T = 3- compared to T = 4-VLP (triangulation number) capsids and profound impairment of accessibility of hexahistidine-peptides in assembled VLPs. Histidines attached to the C-terminus were associated with superior mechanical and/or chemical particle stability depending on the number of histidine moieties. A molecular modeling approach based on cryo-electron microscopy and biolayer interferometry revealed the underlying structural mechanism for the strengthening of the integrity of VLPs. Interactions triggering capsid stabilization occur on a highly conserved residue on the basis of HBcAg-monomers as well as on hexahistidine-peptides of adjacent monomers. This new stabilization mechanism appears to mimic an evolutionary conserved stabilization concept for hepadnavirus core proteins. CONCLUSIONS: These findings establish the genetically simply transferable C-terminal polyhistidine-peptide as a general stabilizing element for chimeric HBcAg-VLPs to increase their suitability.[/su_spoiler] Bidmon N, Kind S, Welters MJP, Joseph-Pietras D, Laske K, Maurer D, Hadrup SR, Schreibelt G, Rae R, Sahin U, Gouttefangeas C, Britten CM, van der Burg SH (2018) Development of an RNA-based kit for easy generation of TCR-engineered lymphocytes to control T-cell assay performance. J Immunol Methods. pii: S0022-1759(18)30025-5 DOI; PMID [su_spoiler title=Show abstract]Cell-based assays to monitor antigen-specific T-cell responses are characterized by their high complexity and should be conducted under controlled conditions to lower multiple possible sources of assay variation. However, the lack of standard reagents makes it difficult to directly compare results generated in one lab over time and across institutions. Therefore TCR-engineered reference samples (TERS) that contain a defined number of antigen-specific T cells and continuously deliver stable results are urgently needed. We successfully established a simple and robust TERS technology that constitutes a useful tool to overcome this issue for commonly used T-cell immuno-assays. To enable users to generate large-scale TERS, on-site using the most commonly used electroporation (EP) devices, an RNA-based kit approach, providing stable TCR mRNA and an optimized manufacturing protocol were established. In preparation for the release of this immuno-control kit, we established optimal EP conditions on six devices and initiated an extended RNA stability study. Furthermore, we coordinated on-site production of TERS with 4 participants. Finally, a proficiency panel was organized to test the unsupervised production of TERS at different laboratories using the kit approach. The results obtained show the feasibility and robustness of the kit approach for versatile in-house production of cellular control samples.[/su_spoiler] Boegel S, Löwer M, Bukur T, Sorn P, Castle J, Sahin U. (2018) HLA and proteasome expression body map. BMC Medical Genomics. 11(1):36. DOI; PMID [su_spoiler title=Show abstract]Background: The presentation of HLA peptide complexes to T cells is a highly regulated and tissue specific process involving multiple transcriptionally controlled cellular components. The extensive polymorphism of HLA genes and the complex composition of the proteasome make it difficult to map their expression profiles across tissues. Methods: Here we applied a tailored gene quantification pipeline to 4323 publicly available RNA-Seq datasets representing 55 normal tissues and cell types to examine expression profiles of (classical and non-classical) HLA class I, class II and proteasomal genes. Results: We generated the first comprehensive expression atlas of antigen presenting-related genes across 56 normal tissues and cell types, including immune cells, pancreatic islets, platelets and hematopoietic stem cells. We found a surprisingly heterogeneous HLA expression pattern with up to 100-fold difference in intra-tissue median HLA abundances. Cells of the immune system and lymphatic organs expressed the highest levels of classical HLA class I (HLA-A,-B,-C), class II (HLA-DQA1,-DQB1,-DPA1,-DPB1,-DRA,-DRB1) and non-classical HLA class I (HLA-E,-F) molecules, whereas retina, brain, muscle, megakaryocytes and erythroblasts showed the lowest abundance. In contrast, we identified a distinct and highly tissue-restricted expression pattern of the non-classical class I gene HLA-G in placenta, pancreatic islets, pituitary gland and testis. While the constitutive proteasome showed relatively constant expression across all tissues, we found the immunoproteasome to be enriched in lymphatic organs and almost absent in immune privileged tissues. Conclusions: Here, we not only provide a reference catalog of tissue and cell type specific HLA expression, but also highlight extremely variable expression of the basic components of antigen processing and presentation in different cell types. Our findings indicate that low expression of classical HLA class I molecules together with lack of immunoproteasome components as well as upregulation of HLA-G may be of key relevance to maintain tolerance in immune privileged tissues.[/su_spoiler] Shen L, Tenzer S, Storck W, Hobernik D, Raker VK, Fischer K, Decker S, Dzionek A, Krauthäuser S, Diken M, Nikolaev A, Maxeiner J, Schuster P, Kappel C, Verschoor A, Schild H, Grabbe S, Bros M (2018) Protein corona-mediated targeting of nanocarriers to B cells allows redirection of allergic immune responses. J Allergy Clin Immunol. 142(5):1558-1570. DOI; PMID [su_spoiler title=Show abstract]Background: Nanoparticle (NP)–based vaccines are attractive immunotherapy tools because of their capability to codeliver antigen and adjuvant to antigen-presenting cells. Their cellular distribution and serum protein interaction (“protein corona”) after systemic administration and their effect on the functional properties of NPs is poorly understood. Objectives: We analyzed the relevance of the protein corona on cell type–selective uptake of dextran-coated NPs and determined the outcome of vaccination with NPs that codeliver antigen and adjuvant in disease models of allergy. Methods: The role of protein corona constituents for cellular binding/uptake of dextran-coated ferrous nanoparticles (DEX-NPs) was analyzed both in vitro and in vivo. DEX-NPs conjugated with the model antigen ovalbumin (OVA) and immunostimulatory CpG-rich oligodeoxynucleotides were administered to monitor the induction of cellular and humoral immune responses. Therapeutic effects of this DEX-NP vaccine in mouse models of OVA-induced anaphylaxisand allergic asthma were assessed. Results: DEX-NPs triggered lectin-induced complement activation, yielding deposition of activated complement factor 3 on the DEX-NP surface. In the spleen DEX-NPs targeted predominantly B cells through complement receptors 1 and 2. The DEX-NP vaccine elicited much stronger OVA-specific IgG2a production than coadministered soluble OVA plus CpG oligodeoxynucleotides. B-cell binding of the DEX-NP vaccine was critical for IgG2aproduction. Treatment of OVA-sensitized mice with the DEX-NP vaccine prevented induction of anaphylactic shock and allergic asthma accompanied by IgE inhibition. Conclusions: Opsonization of lectin-coated NPs by activated complement components results in selective B-cell targeting. The intrinsic B-cell targeting property of lectin-coated NPs can be exploited for treatment of allergic immune responses[/su_spoiler] Sahin U, Türeci Ö. (2018) Personalized vaccines for cancer immunotherapy. Science. Mar 23; 359(6382):1355-1360. DOI; PMID [su_spoiler title=Show abstract]Cancer is characterized by an accumulation of genetic alterations. Somatic mutations can generate cancer-specific neoepitopes that are recognized by autologous T cells as foreign and constitute ideal cancer vaccine targets. Every tumor has its own unique composition of mutations, with only a small fraction shared between patients. Technological advances in genomics, data science, and cancer immunotherapy now enable the rapid mapping of the mutations within a genome, rational selection of vaccine targets, and on-demand production of a therapy customized to a patient’s individual tumor. First-in-human clinical trials of personalized cancer vaccines have shown the feasibility, safety, and immunotherapeutic activity of targeting individual tumor mutation signatures. With vaccination development being promoted by emerging innovations of the digital age, vaccinating a patient with individual tumor mutations may become the first truly personalized treatment for cancer.[/su_spoiler] Diken M, Chu KK, Brodsky AN. (2018) Translating Science into Survival: Report on the Third International Cancer Immunotherapy Conference. Cancer Immunol Res. 2018 Jan;6(1):10-13. doi: 10.1158/2326-6066.CIR-17-0656. DOI; PMID [su_spoiler title=Show abstract]On September 6 to 9, 2017, in Mainz, Germany, the Third International Cancer Immunotherapy Conference was hosted jointly by the Cancer Research Institute, the Association for Cancer Immunotherapy, the European Academy of Tumor Immunology, and the American Association for Cancer Research. For the third straight year, more than 1,400 people attended the four-day event, which covered the latest advances in cancer immunology and immunotherapy. This report provides an overview of the main topics discussed.[/su_spoiler] [/vc_column_text][/vc_column][vc_column width=1/4][vc_basic_grid post_type=page max_items=10 element_width=12 gap=15 orderby=title item=6829 grid_id=vc_gid:1516780861471-483aff06-d626-10 taxonomies=30][/vc_column][/vc_row]" data-id="sfsi-plus"/><meta property="og:url" content="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/2018-2/" data-id="sfsi-plus"/><meta property="og:title" content="2018" data-id="sfsi-plus"/><link rel="alternate" hreflang="en-US" href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/2018-2/"><link rel="alternate" hreflang="de-DE" href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/2018-2/">  <style type="text/css"> #logo .site-title, #logo .site-description { display:none; } body {background-repeat:no-repeat;background-position:top left;background-attachment:scroll;border-top:0px solid #000000;} #header {background-repeat:no-repeat;background-position:left top;margin-top:0px;margin-bottom:0px;padding-top:40px;padding-bottom:40px;border:0px solid ;} #logo .site-title a {font:bold 40px/1em 'Lato', arial, sans-serif;color:#222222;} #logo .site-description {font:300 13px/1em 'Lato', arial, sans-serif;color:#999999;} #header-container{background-color:#ffffff;}#nav-container{border-top:0px solid #dbdbdb;border-bottom:0px solid #dbdbdb;border-left:none;border-right:none;}#nav-container #navigation ul#main-nav > li:first-child{border-left: 0px solid #dbdbdb;}#footer-widgets-container{background-color:#ffffff}#footer-widgets{border:none;}#footer-container{background-color:#164679}#footer-container{border-top:0px solid #dbdbdb;}#footer {border-width: 0 !important;} body, p { font:300 14px/1.5em 'Lato', arial, sans-serif;color:#555555; } h1 { font:bold 28px/1.2em 'Lato', arial, sans-serif;color:#222222; }h2 { font:bold 24px/1.2em 'Lato', arial, sans-serif;color:#222222; }h3 { font:bold 18px/1.2em 'Lato', arial, sans-serif;color:#164679; }h4 { font:bold 16px/1.2em 'Lato', arial, sans-serif;color:#222222; }h5 { font:bold 14px/1.2em 'Lato', arial, sans-serif;color:#222222; }h6 { font:bold 12px/1.2em 'Lato', arial, sans-serif;color:#222222; } .page-title, .post .title, .page .title {font:bold 28px/1.1em 'Lato', arial, sans-serif;color:#be3026;} .post .title a:link, .post .title a:visited, .page .title a:link, .page .title a:visited {color:#be3026} .post-meta { font:300 12px/1.5em 'Lato', arial, sans-serif;color:#999999; } .entry, .entry p{ font:300 15px/1.5em 'Lato', arial, sans-serif;color:#555555; } .post-more {font:300 13px/1.5em Lato;color:;border-top:0px solid #e6e6e6;border-bottom:0px solid #e6e6e6;} #post-author, #connect {border-top:1px solid #e6e6e6;border-bottom:1px solid #e6e6e6;border-left:1px solid #e6e6e6;border-right:1px solid #e6e6e6;border-radius:5px;-moz-border-radius:5px;-webkit-border-radius:5px;background-color:#fafafa} .nav-entries a, .woo-pagination { font:300 13px/1em Arial, sans-serif;color:#888; } .woo-pagination a, .woo-pagination a:hover {color:#888!important} .widget h3 {font:bold 14px/1.2em Arial, sans-serif;color:#555555;border-bottom:1px solid #e6e6e6;} .widget_recent_comments li, #twitter li { border-color: #e6e6e6;} .widget p, .widget .textwidget { font:300 13px/1.5em Arial, sans-serif;color:#164679; } .widget {font:300 13px/1.5em Arial, sans-serif;color:#164679;border-radius:0px;-moz-border-radius:0px;-webkit-border-radius:0px;} #tabs .inside li a, .widget_woodojo_tabs .tabbable .tab-pane li a { font:bold 12px/1.5em Arial, sans-serif;color:#555555; } #tabs .inside li span.meta, .widget_woodojo_tabs .tabbable .tab-pane li span.meta { font:300 11px/1.5em Arial, sans-serif;color:#999999; } #tabs ul.wooTabs li a, .widget_woodojo_tabs .tabbable .nav-tabs li a { font:300 11px/2em Arial, sans-serif;color:#999999; } @media only screen and (min-width:768px) { ul.nav li a, #navigation ul.rss a, #navigation ul.cart a.cart-contents, #navigation .cart-contents #navigation ul.rss, #navigation ul.nav-search, #navigation ul.nav-search a { font: 14px/1.2em Helvetica, Arial, sans-serif;color:#666666; } #navigation ul.rss li a:before, #navigation ul.nav-search a.search-contents:before { color:#666666;} #navigation ul.nav li ul, #navigation ul.cart > li > ul > div { border: 0px solid #dbdbdb; } #navigation ul.nav > li:hover > ul { left: 0; } #navigation ul.nav > li { border-right: 0px solid #dbdbdb; }#navigation ul.nav > li:hover > ul { left: 0; } #navigation { box-shadow: none; -moz-box-shadow: none; -webkit-box-shadow: none; }#navigation ul li:first-child, #navigation ul li:first-child a { border-radius:0px 0 0 0px; -moz-border-radius:0px 0 0 0px; -webkit-border-radius:0px 0 0 0px; } #navigation {border-top:0px solid #dbdbdb;border-bottom:0px solid #dbdbdb;border-left:0px solid #dbdbdb;border-right:0px solid #dbdbdb;border-radius:0px; -moz-border-radius:0px; -webkit-border-radius:0px;} #top ul.nav li a { font:thin 12px/1.6em Helvetica, Arial, sans-serif;color:#ddd; } } #footer, #footer p { font:300 13px/1.4em 'Lato', arial, sans-serif;color:#ffffff; } #footer {border-top:0px solid #dbdbdb;border-bottom:0px solid ;border-left:0px solid ;border-right:0px solid ;border-radius:0px; -moz-border-radius:0px; -webkit-border-radius:0px;} .magazine #loopedSlider .content h2.title a { font:normal 24px/1em 'Lato', arial, sans-serif;color:#555555; } .wooslider-theme-magazine .slide-title a { font:normal 24px/1em 'Lato', arial, sans-serif;color:#555555; } .magazine #loopedSlider .content .excerpt p { font:normal 14px/1.5em 'Lato', arial, sans-serif;color:#555555; } .wooslider-theme-magazine .slide-content p, .wooslider-theme-magazine .slide-excerpt p { font:normal 14px/1.5em 'Lato', arial, sans-serif;color:#555555; } .magazine .block .post .title a {font:normal 18px/1.2em 'Lato', arial, sans-serif;color:#555555; } #loopedSlider.business-slider .content h2 { font:bold 24px/1em Arial, sans-serif;color:#ffffff; } #loopedSlider.business-slider .content h2.title a { font:bold 24px/1em Arial, sans-serif;color:#ffffff; } .wooslider-theme-business .has-featured-image .slide-title { font:bold 24px/1em Arial, sans-serif;color:#ffffff; } .wooslider-theme-business .has-featured-image .slide-title a { font:bold 24px/1em Arial, sans-serif;color:#ffffff; } #wrapper #loopedSlider.business-slider .content p { font:thin 13px/1.5em Arial, sans-serif;color:#cccccc; } .wooslider-theme-business .has-featured-image .slide-content p { font:thin 13px/1.5em Arial, sans-serif;color:#cccccc; } .wooslider-theme-business .has-featured-image .slide-excerpt p { font:thin 13px/1.5em Arial, sans-serif;color:#cccccc; } .archive_header { font:bold 18px/1em 'Lato', arial, sans-serif;color:#222222; } .archive_header {border-bottom:1px solid #e6e6e6;} </style>  <link rel="shortcut icon" href="https://web.archive.org/web/20210320060823im_/https://tron-mainz.de/de/wp-content/uploads/sites/9/2017/03/tron-favicon.png"/>  <link href="https://web.archive.org/web/20210320060823cs_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/themes/canvas/functions/css/shortcodes_css.css" rel="stylesheet" type="text/css"/>  <link href="https://web.archive.org/web/20210320060823cs_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/themes/canvas/custom_css.css" rel="stylesheet" type="text/css"/>  <meta name="generator" content="Canvas 5.9.19"/> <meta name="generator" content="WooFramework 6.2.7"/>  <link href="https://web.archive.org/web/20210320060823cs_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/fonts.googleapis.com/css_family_Lato_3A100_2C147212186.css" rel="stylesheet" type="text/css"/> <meta name="generator" content="Powered by WPBakery Page Builder - drag and drop page builder for WordPress."/> <style type="text/css" id="woo-header-css"> #logo .site-title a { color: #ffffff; } </style> <style> @media only screen and ( min-width: 768px ) { #nav-container { -moz-box-shadow: 0px 0px 0px #515151 !important; -webkit-box-shadow: 0px 0px 0px #515151 !important; box-shadow: 0px 0px 0px #515151 !important; } .primary-nav-container, .side-nav, #navigation ul.nav li a, #navigation ul.rss a, #navigation ul.cart a.cart-contents, #navigation .cart-contents #navigation ul.rss, #navigation ul.nav-search, #navigation ul.nav-search a { font-family: Lato ; font-size: 1.1em ; font-style: normal ; font-weight: 700; color : #164679 !important; } #navigation > section > .primary-nav-container ul.nav > .current-menu-item > a, #navigation > section > .primary-nav-container ul.nav > .current-menu-parent > a { color: #be3026 !important ; } #navigation > section > .primary-nav-container ul.nav > .menu-item.current-menu-item { background-color: #ffffff ; } #navigation > section > .primary-nav-container ul.nav > .current-menu-item > a { background-color: transparent ; } #navigation > section > .primary-nav-container ul.nav > .menu-item { background-color: #ffffff ; } #navigation .side-nav > ul > li > a { background-color: #ffffff !important ;color: #164679 !important; } #navigation > section > .primary-nav-container ul.nav > .menu-item > a { background-color: transparent ; } #navigation ul.nav > li:hover > a, #navigation .side-nav > ul > li:hover > a { color: #9b0000 !important; background: !important; } #navigation .side-nav > ul > li > a:before { color: inherit !important; } #navigation > section > .primary-nav-container ul.nav > .menu-item > a { text-shadow: inherit !important; } #navigation > section > .primary-nav-container ul.nav > .menu-item { } #navigation > section > .primary-nav-container ul.nav > .menu-item:hover { } #navigation > section > .primary-nav-container ul.nav { display: inline-block; float: none !important ; } #navigation > section > .primary-nav-container ul.nav > li.menu-item { display: inline-block !important; float: none !important; vertical-align: middle !important; } #navigation > section { height: auto; text-align: right !important ; } #navigation > section > .primary-nav-container ul.nav { display: inline-block; float: none !important ; } #navigation > section > .primary-nav-container ul.nav > li.menu-item { display: inline-block !important; float: none !important; vertical-align: middle !important; } #navigation > section { height: 42px; text-align: right !important ; } #navigation > section > .primary-nav-container ul.nav > .menu-item > a { padding-top: px !important ; padding-bottom: px !important ; padding-right: px !important ; padding-left: px !important ; } #navigation > section > .primary-nav-container ul.nav > .menu-item { margin-top: px !important ; margin-bottom: px !important ; margin-right: px !important ; margin-left: px !important ; } #navigation > section > .primary-nav-container ul.nav > .menu-item, #navigation > section > .primary-nav-container ul.nav > .menu-item > a { border-radius: !important ; } #navigation > section > .primary-nav-container ul.nav > .menu-item { border: none !important; } #navigation > section > .primary-nav-container ul.nav > .menu-item:hover, #navigation > section > .primary-nav-container ul.nav > .menu-item.hover { border-color: !important; } #navigation > section > .primary-nav-container ul.nav > .menu-item.current-menu-item { border-color: #be3026 !important; } #navigation > section > .primary-nav-container ul.nav > .menu-item- > a { font-family: ; font-size: px ; font-style: normal ; font-weight: 400; color : !important; } #navigation > section > .primary-nav-container ul.nav > .menu-item-.current-menu-item > a { color: !important ; } #navigation > section > .primary-nav-container ul.nav > .menu-item-.current-menu-item { background-color: ; } #navigation > section > .primary-nav-container ul.nav > .menu-item-:hover > a { color: !important ; background-color: !important ; } #navigation > section > .primary-nav-container ul.nav > .menu-item- { background-color: ; } #navigation > section > .primary-nav-container ul.nav > .menu-item- { } #navigation > section > .primary-nav-container ul.nav > .menu-item- > a { padding: px px !important ; } #navigation > section > .primary-nav-container ul.nav > .menu-item- { margin: px px !important ; } #navigation > section > .primary-nav-container ul.nav > .menu-item- { border-radius: !important ; border-top: px solid ; border-bottom: px solid ; border-left: px solid ; border-right: px solid ; } #navigation > section > .primary-nav-container ul.nav > .menu-item-.current-menu-item:hover, #navigation > section > .primary-nav-container ul.nav > .menu-item-:hover { border-top: px solid ; border-bottom: px solid ; border-left: px solid ; border-right: px solid ; } #navigation > section > .primary-nav-container ul.nav > .menu-item-.current-menu-item { border-top: px solid ; border-bottom: px solid ; border-left: px solid ; border-right: px solid ; } #navigation > section > .primary-nav-container ul.nav > .menu-item .sub-menu, #navigation > section > .primary-nav-container ul.nav > .menu-item .sub-menu > .menu-item > a, #navigation > section > .side-nav .widget_shopping_cart, #navigation ul.cart .cart_list a { font-family: ; font-size: 1em ; font-style: normal ; font-weight: 400; color : !important; } #navigation > section > .primary-nav-container ul.nav > .menu-item .sub-menu > .menu-item > a:hover, #navigation > section > .side-nav .widget_shopping_cart { text-decoration: none; } #navigation > section > .primary-nav-container ul.nav .sub-menu { text-align: left !important ; } #navigation > section > .primary-nav-container ul.nav > .menu-item .sub-menu > .menu-item { border-top: px solid ; border-bottom: px solid ; border-left: px solid ; border-right: px solid ; } #navigation > section > .primary-nav-container ul.nav > .menu-item .sub-menu > .menu-item:hover { border-top: px solid ; border-bottom: px solid ; border-left: px solid ; border-right: px solid ; } #navigation > section > .primary-nav-container ul.nav > .menu-item .sub-menu, #navigation .side-nav > .cart > li > ul, #navigation .side-nav > .nav-search > li > ul { background-color: #ffffff !important; } #navigation > section > .primary-nav-container ul.nav > .menu-item .sub-menu > .menu-item:hover > a { color: #be3026 !important; } #navigation > section > .primary-nav-container ul.nav > .menu-item .sub-menu { padding-top: 0 !important; padding-bottom: 0 !important; } #navigation > section > .primary-nav-container ul.nav .sub-menu > .menu-item > a, #navigation > section > .side-nav .widget_shopping_cart { } #navigation > section > .primary-nav-container ul.nav .sub-menu, #navigation > section > .side-nav > .cart > li > ul { } body #navigation > section > .primary-nav-container ul.nav > .menu-item .sub-menu, #navigation > section > .side-nav > .cart > li > ul { border-radius: !important ; } #navigation > section > .primary-nav-container ul.nav > .menu-item .sub-menu > .menu-item:first-child { border-radius: 0 0 !important ; } #navigation > section > .primary-nav-container ul.nav > .menu-item .sub-menu > .menu-item:last-child { border-radius: 0 0 !important ; } #navigation > section > .primary-nav-container ul.nav { display: inline-block; vertical-align: middle; } #navigation > section > .side-nav { height:100%; vertical-align: middle; float:right; } #nav-container, #navigation { background-color: #ffffff !important; } #nav-container {border: none !important;}#nav-container { -moz-border-radius: 3px !important; -webkit-border-radius: 3px !important; border-radius: 3px !important; } #navigation ul.nav li ul { width:168px; font-size:14px; }#navigation ul.nav li { line-height:1.2em; }#navigation ul .sub-menu > li:first-child, #navigation ul .sub-menu > li:first-child > a { border-radius: 0 !important; -moz-border-radius: 0 !important; -webkit-border-radius: 0 !important; } #navigation { margin-top:0;margin-bottom:0; } #nav-container { margin-top: px; margin-bottom: px; } #navigation ul.nav > li > a { } #navigation ul.nav > li > a > i { margin-right: 5px; line-height: inherit; vertical-align: middle; color: ; } #navigation ul.nav > li > a:hover > i { color: ; } #navigation ul.nav > li.current-menu-item > a > i { color: ; } #navigation ul.nav > li ul li > a { line-height: ; } #navigation ul.nav > li ul li > a > i { float: left; margin-right: 5px; line-height: inherit; color: ; text-decoration: initial; } #nav-container #navigation ul#main-nav > li:first-child { border-left: none; } #navigation { transform: initial !important; }.primary-nav-container { display: inline-block; }#navigation ul.rss li.sub-rss a i,#navigation ul.rss li.sub-email a i{display:none;}body #navigation > section > .primary-nav-container ul.nav > .menu-item > .sub-menu > .menu-item:first { border-radius: 0 !important; } #navigation #main-nav > .menu-item > .sub-menu:before { content: ''; } #navigation #main-nav { font-size: 0; } #navigation #main-nav li { font-size: 14px; } #navigation #main-nav > .menu-item.parent:after { content: ''; position: absolute; top: 100%; display: none; } #navigation #main-nav > .menu-item.parent:hover:after { content: ''; position: absolute; top: 100%; display: block; } #navigation > section { height: auto; }#navigation .side-nav > .rss { position: relative; line-height: 100%; height: 100%; } #navigation .side-nav > .rss > li { line-height: 100%; height: 100%; } #navigation .side-nav > .rss > li > a { padding-top: 0; padding-bottom: 0; line-height: 100%; height: 100%; position: relative; overflow:hidden; } #navigation .side-nav > .rss > li > a:hover { text-decoration: none; } #navigation .side-nav > ul > li > a:before { position: absolute !important; top: 50% !important; margin-top: -0.5em !important; line-height:1; } #navigation .side-nav > .cart { line-height: 100%; height: 100% } #navigation .side-nav > .cart > li { height: 100% } #navigation .side-nav > .cart > li > .cart-contents { padding-top: 0; padding-bottom: 0; line-height: 100%; height: 100%; position: relative; } @media only screen and (min-width: 980px) { #navigation .side-nav > .cart > li { display: table; } #navigation .side-nav > .cart > li > a.cart-contents { display: table-cell; vertical-align: middle; } #navigation .side-nav > .cart > li > a.cart-contents:before { position: initial !important; } } #navigation ul.cart a.cart-contents + ul { box-sizing: content-box; } #navigation .side-nav > .nav-search { line-height: 100%; height: 100% } #navigation .side-nav > .nav-search > li { height: 100% } #navigation .side-nav > .nav-search > li > .search-contents { padding-top: 0; padding-bottom: 0; line-height: 100%; height: 100%; position: relative } #navigation .side-nav > ul > li > a:before { color: #be3026 !important; } #navigation .side-nav > ul > li > a:hover:before { color: !important; } #navigation #main-nav > .menu-item > a > span { vertical-align: middle; } .side-nav ul ul input , .side-nav ul ul :before { color: !important ; } } @media only screen and ( max-width: 767px ) { #navigation ul.nav > li > a > i { display: none; } #navigation ul.nav > li ul li > a > i { display: none } } </style> <style type="text/css">/* *** NEWS BOX - WP DYNAMIC CUSTOM THEME *** */ .lcnb_wpdt_theme .lcnb_loading { background: url('https://web.archive.org/web/20210320060823im_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/swift_box_wp/js/sb//img/loader_d_gif.gif') no-repeat center center transparent; } .lcnb_wpdt_theme article.lcnb_news, .lcnb_wpdt_theme .lcnb_exp_block { background-color: #ffffff; } .lcnb_wpdt_theme.lcnb_wrap.lcnb_uniblock .lcnb_inner_wrapper { border-radius: 0px; } .lcnb_wpdt_theme.lcnb_wrap.lcnb_uniblock .lcnb_news, .lcnb_wpdt_theme.lcnb_wrap.lcnb_boxed article.lcnb_news, .lcnb_wpdt_theme.lcnb_wrap.lcnb_uniblock .lcnb_exp_block { border: 0px solid #D5D5D5; -moz-transition: box-shadow .2s linear, border-color .2s linear; -webkit-transition: box-shadow .2s linear, border-color .2s linear; -o-transition: box-shadow .2s linear, border-color .2s linear; -ms-transition: box-shadow .2s linear, border-color .2s linear; transition: box-shadow .2s linear, border-color .2s linear; } .lcnb_wpdt_theme.lcnb_wrap.lcnb_uniblock .lcnb_news:hover, .lcnb_wpdt_theme.lcnb_wrap.lcnb_boxed article.lcnb_news:hover { border-color: #C4C4C4; } /* IMPORANT - USE THE SAME VALUE OF THE .lcnb_news BORDER */ .lcnb_wpdt_theme.lcnb_vertical.lcnb_wrap.lcnb_uniblock .lcnb_news { margin-top: -0px; } .lcnb_wpdt_theme.lcnb_vertical.lcnb_wrap.lcnb_uniblock .lcnb_news:first-child { margin-top: 0px; } .lcnb_wpdt_theme.lcnb_horizontal.lcnb_wrap.lcnb_uniblock .lcnb_news { margin-left: -0px; } .lcnb_wpdt_theme.lcnb_horizontal.lcnb_wrap.lcnb_uniblock .lcnb_news:first-child { margin-left: 0px; } /* **** */ .lcnb_wpdt_theme.lcnb_wrap.lcnb_boxed article.lcnb_news, .lcnb_wpdt_theme.lcnb_boxed .lcnb_exp_block { border: 0px solid #D5D5D5; border-radius: 0px; box-shadow: none; } .lcnb_wpdt_theme .lcnb_title { color: #164679 !important; border-bottom: 1px solid #FFFFFF; font-weight: bold; } .lcnb_wpdt_theme .lcnb_txt, .lcnb_wpdt_theme .lcnb_exp_txt { color: #555555; } .lcnb_wpdt_theme .lcnb_txt a { color: #be3026 !important; } .lcnb_wpdt_theme .lcnb_img { background: url('https://web.archive.org/web/20210320060823im_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/swift_box_wp/js/sb//img/loader_d_gif.gif') no-repeat center center transparent; } .lcnb_wpdt_theme .lcnb_social_box li { color: #164679; } /* navigation commands */ .lcnb_wpdt_theme.lcnb_has_cmd .lcnb_prev, .lcnb_wpdt_theme.lcnb_has_cmd .lcnb_next { border: 1px solid #D5D5D5; background-color: #ffffff; } .lcnb_wpdt_theme.lcnb_has_cmd .lcnb_prev:hover, .lcnb_wpdt_theme.lcnb_has_cmd .lcnb_next:hover { border-color: #C4C4C4; } .lcnb_wpdt_theme .lcnb_cmd span:before { color: #164679; } /* source logos */ .lcnb_wpdt_theme.lcnb_src_logo .lcnb_type_twitter > div { background-image: url("https://web.archive.org/web/20210320060823im_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/swift_box_wp/js/sb//img/social_src_logos/dark/twitter_png.png"); } .lcnb_wpdt_theme.lcnb_src_logo .lcnb_type_rss > div { background-image: url("https://web.archive.org/web/20210320060823im_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/swift_box_wp/js/sb//img/social_src_logos/dark/rss_png.png"); } .lcnb_wpdt_theme.lcnb_src_logo .lcnb_type_pinterest > div { background-image: url("https://web.archive.org/web/20210320060823im_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/swift_box_wp/js/sb//img/social_src_logos/dark/pinterest_png.png"); } .lcnb_wpdt_theme.lcnb_src_logo .lcnb_type_facebook > div { background-image: url("https://web.archive.org/web/20210320060823im_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/swift_box_wp/js/sb//img/social_src_logos/dark/facebook_png.png"); } .lcnb_wpdt_theme.lcnb_src_logo .lcnb_type_google > div { background-image: url("https://web.archive.org/web/20210320060823im_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/swift_box_wp/js/sb//img/social_src_logos/dark/google_png.png"); } .lcnb_wpdt_theme.lcnb_src_logo .lcnb_type_youtube > div { background-image: url("https://web.archive.org/web/20210320060823im_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/swift_box_wp/js/sb//img/social_src_logos/dark/youtube_png.png"); } .lcnb_wpdt_theme.lcnb_src_logo .lcnb_type_soundcloud > div { background-image: url("https://web.archive.org/web/20210320060823im_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/swift_box_wp/js/sb//img/social_src_logos/dark/soundcloud_png.png"); } .lcnb_wpdt_theme.lcnb_src_logo .lcnb_type_tumblr > div { background-image: url("https://web.archive.org/web/20210320060823im_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/swift_box_wp/js/sb//img/social_src_logos/dark/tumblr_png.png"); } /* bottom ant top bar */ .lcnb_wpdt_theme .lcnb_btm_bar .lcnb_date, .lcnb_wpdt_theme .lcnb_top_bar .lcnb_date, .lcnb_wpdt_theme .lcnb_exp_date time, .lcnb_wpdt_theme .lcnb_btm_bar .lcnb_rm_btn, .lcnb_wpdt_theme .lcnb_top_bar .lcnb_rm_btn { background-color: #F3F3F3; color: #164679; padding: 4px 8px; border-radius: 2px; } .lcnb_wpdt_theme .lcnb_btm_bar .lcnb_link, .lcnb_wpdt_theme .lcnb_top_bar .lcnb_link, .lcnb_wpdt_theme .lcnb_btm_bar .lcnb_social_trigger, .lcnb_wpdt_theme .lcnb_top_bar .lcnb_social_trigger, .lcnb_wpdt_theme .lcnb_btm_bar .lcnb_btn_expand, .lcnb_wpdt_theme .lcnb_top_bar .lcnb_btn_expand { border-right: 1px solid #FFFFFF; box-shadow: none; } .lcnb_wpdt_theme .lcnb_btm_bar .lcnb_link, .lcnb_wpdt_theme .lcnb_top_bar .lcnb_link, .lcnb_wpdt_theme .lcnb_btm_bar .lcnb_social_trigger, .lcnb_wpdt_theme .lcnb_top_bar .lcnb_social_trigger, .lcnb_wpdt_theme .lcnb_btm_bar .lcnb_btn_expand, .lcnb_wpdt_theme .lcnb_top_bar .lcnb_btn_expand { color: #164679; } .lcnb_wpdt_theme .lcnb_btm_bar.lcnb_narrow_txt, .lcnb_wpdt_theme .lcnb_top_bar.lcnb_narrow_txt { background: #ffffff; background: rgba(255,255,255, 0.9); } .lcnb_wpdt_theme .lcnb_btm_bar .lcnb_link, .lcnb_wpdt_theme .lcnb_top_bar .lcnb_link, .lcnb_wpdt_theme .lcnb_btm_bar .lcnb_social_trigger, .lcnb_wpdt_theme .lcnb_top_bar .lcnb_social_trigger, .lcnb_wpdt_theme .lcnb_btm_bar .lcnb_btn_expand, .lcnb_wpdt_theme .lcnb_top_bar .lcnb_btn_expand { opacity: 0.8; filter: alpha(opacity=80); } .lcnb_wpdt_theme .lcnb_rm_btn:hover { background-color: #E8E8E8 !important; color: #303030 !important; } /* social flap */ .lcnb_wpdt_theme .lcnb_btm_bar .lcnb_social_box, .lcnb_wpdt_theme .lcnb_top_bar .lcnb_social_box, .lcnb_wpdt_theme .lcnb_exp_data .lcnb_social_box { background-color: #ffffff; border: 1px solid #D5D5D5; } .lcnb_wpdt_theme .lcnb_btm_bar .lcnb_social_box:after, .lcnb_wpdt_theme .lcnb_exp_data .lcnb_social_box:after { border-top-color: #D5D5D5; } .lcnb_wpdt_theme .lcnb_top_bar .lcnb_social_box:before { border-bottom-color: #D5D5D5; } .lcnb_wpdt_theme .lcnb_btm_bar .lcnb_social_box > li, .lcnb_wpdt_theme .lcnb_top_bar .lcnb_social_box > li, .lcnb_wpdt_theme .lcnb_exp_data .lcnb_social_box > li { border-bottom: 1px solid #D5D5D5; } /* lightbox overlays */ .lcnb_wpdt_theme .lcnb_lb_icon:before { color: #333333; } .lcnb_wpdt_theme .lcnb_lb_overlay { background-color: #FFFFFF; opacity: 0.7; filter: alpha(opacity=70); } /*** vertical ***/ .lcnb_wpdt_theme.lcnb_vertical.lcnb_side_cmd .lcnb_prev span { background-position: -132px 7px; } .lcnb_wpdt_theme.lcnb_vertical.lcnb_side_cmd .lcnb_next span { background-position: -168px 5px; } .lcnb_wpdt_theme.lcnb_vertical .lcnb_img { border-width: 0px 0px 0px 0px; border-style: solid; border-color: transparent; } .lcnb_wpdt_theme.lcnb_vertical .lcnb_img .lcnb_img_lb, .lcnb_wpdt_theme.lcnb_vertical .lcnb_img .lcnb_video_lb { margin: 0px 0 0 0px; /* IMPORANT - set the same value as .lcnb_img border */ } .lcnb_wpdt_theme.lcnb_vertical .lcnb_buttons { background-color: #ffffff; background-color: rgba(255,255,255, 0.85); border-right: 1px solid #FFFFFF; border-radius: 0px 0 0 0px; } .lcnb_wpdt_theme.lcnb_vertical .lcnb_buttons > div { border-color: #FFFFFF; border-left-color: #FFFFFF; opacity: 0.9; filter: alpha(opacity=90); } .lcnb_wpdt_theme.lcnb_vertical .lcnb_buttons > div:hover { opacity: 1; filter: alpha(opacity=100); } .lcnb_wpdt_theme.lcnb_vertical .lcnb_buttons { color: #164679; } .lcnb_wpdt_theme.lcnb_vertical .lcnb_buttons .lcnb_social_trigger.socials_shown { background-color: #ffffff; } .lcnb_wpdt_theme.lcnb_vertical .lcnb_buttons ul.lcnb_social_box { background-color: #ffffff; border: 1px solid #FFFFFF; } .lcnb_wpdt_theme.lcnb_vertical .lcnb_buttons .lcnb_social_box li { border-right: 1px solid #FFFFFF; } .lcnb_wpdt_theme.lcnb_vertical .lcnb_buttons .lcnb_social_box li:last-child { border-right: none !important; } .lcnb_wpdt_theme.lcnb_vertical .lcnb_buttons > div:hover, .lcnb_wpdt_theme.lcnb_vertical .lcnb_buttons > div.lcnb_active { background-color: #ffffff; } /*** horizontal ***/ .lcnb_wpdt_theme.lcnb_horizontal.lcnb_side_cmd .lcnb_prev span { background-position: -85px center; } .lcnb_wpdt_theme.lcnb_horizontal.lcnb_side_cmd .lcnb_next span { background-position: -115px center; } .lcnb_wpdt_theme.lcnb_horizontal .lcnb_img { border-width: 0px 0px 0; border-style: solid; border-color: transparent; } /**** EXPANDED NEWS ****/ .lcnb_wpdt_theme .lcnb_exp_block .lcnb_close:before { color: #164679; } .lcnb_wpdt_theme .lcnb_exp_data { border-top: 1px solid #FFFFFF; } .lcnb_wpdt_theme .lcnb_exp_data .lcnb_social_trigger, .lcnb_wpdt_theme .lcnb_exp_data .lcnb_link { border-left: 1px solid #FFFFFF; color: #164679; } .lcnb_wpdt_theme .lcnb_exp_img_wrap, .lcnb_wpdt_theme .lcnb_exp_body_img > div:first-child { border: 3px solid #FFFFFF; box-shadow: 0 0 1px #FFFFFF; } </style><style type="text/css">.entry img {padding: 0px;border: none;} .lcnb_wpdt_theme .lcnb_title {border-bottom: none;} .lcnb_wpdt_theme.lcnb_has_cmd .lcnb_prev, .lcnb_wpdt_theme.lcnb_has_cmd .lcnb_next {background-color: #f3f3f3!important;border: none!important;} .lcnb_horizontal.lcnb_side_cmd .lcnb_prev, .lcnb_horizontal.lcnb_side_cmd .lcnb_next {border-radius: 3px;} .lcnb_lb_icon {display:none;} .lcnb_lb_overlay {display:none;} .lcnb_horizontal.lcnb_has_cmd.lcnb_side_cmd {margin: 0!important;} .entry p {color: #164679;} .lcnb_wpdt_theme .lcnb_cmd span:before {color: #555555!important;} .lcnb_horizontal.lcnb_side_cmd .lcnb_next, .lcnb_horizontal.lcnb_side_cmd .lcnb_prev {height: 44px;} .lcnb_wpdt_theme.lcnb_has_cmd .lcnb_prev:hover, .lcnb_wpdt_theme.lcnb_has_cmd .lcnb_next:hover {background-color: #be3026!important;border: none!important;} .lcnb_horizontal.lcnb_side_cmd .lcnb_next, .lcnb_horizontal.lcnb_side_cmd .lcnb_prev {margin-top: 100px;} .lcnb_horizontal .lcnb_btm_bar {padding: 0px 6px 7px 10px;} .lcnb_horizontal .lcnb_contents {padding: 6px 14px 0px;}.lcnb_linked_title, .lcnb_title {font-size: 16px;} .lcnb_txt {font-size: 14px;} </style><noscript><style> .wpb_animate_when_almost_visible { opacity: 1; }</style></noscript></head> <body data-rsssl="1" class="page-template-default page page-id-8451 chrome alt-style-default one-col width-1200 one-col-1200 full-width full-header full-footer wpb-js-composer js-comp-ver-6.6.0 vc_responsive"> <?php echo DISPLAY_ULTIMATE_PLUS(); ?> <div id="wrapper"> <div id="inner-wrapper">  <div id="header-container"> <h3 class="nav-toggle icon"><a href="#navigation">Navigation</a></h3> <header id="header" class="col-full"> <div class="sfsi_plus_widget"><div id="sfsi_plus_wDiv"></div><div class="sfsiplus_norm_row sfsi_plus_wDiv" style="width:150px;position:absolute;;text-align:right"><div style="width:25px; height:25px;margin-left:5px;margin-bottom:5px;" class="sfsi_plus_wicons shuffeldiv "><div class="sfsiplus_inerCnt"><a class=" sficn" data-effect="" target="_blank" href="https://web.archive.org/web/20210320060823/https://twitter.com/tronmainz" style="opacity:1"><img alt="Twitter" title="Twitter" src="https://web.archive.org/web/20210320060823im_/https://tron-mainz.de/de/wp-content/plugins/ultimate-social-media-plus/images/icons_theme/flat/flat_twitter.png" width="25" height="25" style="" class="sfcm sfsi_wicon sfsiplusid_round_icon_twitter" data-effect=""/></a></div></div><div style="width:25px; height:25px;margin-left:5px;margin-bottom:5px;" class="sfsi_plus_wicons shuffeldiv "><div class="sfsiplus_inerCnt"><a class=" sficn" data-effect="" target="_blank" href="https://web.archive.org/web/20210320060823/https://www.linkedin.com/company/1284674" style="opacity:1"><img alt="LinkedIn" title="LinkedIn" src="https://web.archive.org/web/20210320060823im_/https://tron-mainz.de/de/wp-content/plugins/ultimate-social-media-plus/images/icons_theme/flat/flat_linkedin.png" width="25" height="25" style="" class="sfcm sfsi_wicon sfsiplusid_round_icon_linkedin" data-effect=""/></a></div></div><div style="width:25px; height:25px;margin-left:5px;margin-bottom:5px;" class="sfsi_plus_wicons shuffeldiv cmcls"><div class="sfsiplus_inerCnt"><a class=" sficn" data-effect="" target="_blank" href="/web/20210320060823/https://tron-mainz.de/de/kontakt" style="opacity:1"><img alt="SOCIALICON" title="SOCIALICON" src="https://web.archive.org/web/20210320060823im_/https://tron-mainz.de/de/wp-content/uploads/sites/9/2019/03/plus_custom_icon1.png" width="25" height="25" style="" class="sfcm sfsi_wicon sfsiplusid_round_icon_custom" data-effect=""/></a></div></div></div><div id="sfsi_holder" class="sfsi_plus_holders" style="position: relative; float: left;width:100%;z-index:-1;"></div><script></script><div style="clear: both;"></div></div> <div id="logo"> <a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/" title=""><img src="https://web.archive.org/web/20210320060823im_/https://tron-mainz.de/de/wp-content/uploads/sites/9/2020/07/TRON_Logo_Science.svg" alt="TRON Translational Oncology Mainz | Connecting Competence"/></a> <span class="site-title"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/">TRON Translational Oncology Mainz | Connecting Competence</a></span> </div> <div class="header-widget"> <div id="sfsi-plus-widget-2" class="widget sfsi_plus"> <div class="sfsi_plus_widget" data-position="widget"> <div id="sfsi_plus_wDiv"></div> <script> window.addEventListener('sfsi_plus_functions_loaded', function() { if (typeof sfsi_plus_widget_set == 'function') { sfsi_plus_widget_set(); } }) </script> <div class="sfsiplus_norm_row sfsi_plus_wDiv" style="width:150px;position:absolute;;text-align:right"><div style="width:25px; height:25px;margin-left:5px;margin-bottom:5px;" class="sfsi_plus_wicons shuffeldiv "><div class="sfsiplus_inerCnt"><a class=" sficn" data-effect="" target="_blank" href="https://web.archive.org/web/20210320060823/https://twitter.com/tronmainz" style="opacity:1"><img alt="Twitter" title="Twitter" src="https://web.archive.org/web/20210320060823im_/https://tron-mainz.de/de/wp-content/plugins/ultimate-social-media-plus/images/icons_theme/flat/flat_twitter.png" width="25" height="25" style="" class="sfcm sfsi_wicon sfsiplusid_round_icon_twitter" data-effect=""/></a></div></div><div style="width:25px; height:25px;margin-left:5px;margin-bottom:5px;" class="sfsi_plus_wicons shuffeldiv "><div class="sfsiplus_inerCnt"><a class=" sficn" data-effect="" target="_blank" href="https://web.archive.org/web/20210320060823/https://www.linkedin.com/company/1284674" style="opacity:1"><img alt="LinkedIn" title="LinkedIn" src="https://web.archive.org/web/20210320060823im_/https://tron-mainz.de/de/wp-content/plugins/ultimate-social-media-plus/images/icons_theme/flat/flat_linkedin.png" width="25" height="25" style="" class="sfcm sfsi_wicon sfsiplusid_round_icon_linkedin" data-effect=""/></a></div></div><div style="width:25px; height:25px;margin-left:5px;margin-bottom:5px;" class="sfsi_plus_wicons shuffeldiv cmcls"><div class="sfsiplus_inerCnt"><a class=" sficn" data-effect="" target="_blank" href="/web/20210320060823/https://tron-mainz.de/de/kontakt" style="opacity:1"><img alt="SOCIALICON" title="SOCIALICON" src="https://web.archive.org/web/20210320060823im_/https://tron-mainz.de/de/wp-content/uploads/sites/9/2019/03/plus_custom_icon1.png" width="25" height="25" style="" class="sfcm sfsi_wicon sfsiplusid_round_icon_custom" data-effect=""/></a></div></div></div><div id="sfsi_holder" class="sfsi_plus_holders" style="position: relative; float: left;width:100%;z-index:-1;"></div><script></script> <div style="clear: both;"></div> </div> </div><div id="mlp_widget-3" class="widget mlp_widget"><div class="mlp-language-box mlp_language_box"><ul><li><a rel="alternate" href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/2018-2/?noredirect=en_US">en</a></li></ul></div></div> </div>  <div id="nav-container"> <nav id="navigation" class="col-full" role="navigation"> <section class="menus nav-icons nav-icons-1"> <div class="primary-nav-container"> <a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de" class="nav-home"> <i class="fa fa-home"></i> <span>Home</span></a> <h3>main</h3><ul id="main-nav" class="nav fl"><li id="menu-item-7350" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-has-children menu-item-7350"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/ueber-tron/"><span>Über TRON</span></a> <ul class="sub-menu"> <li id="menu-item-7351" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-7351"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/ueber-tron/"><span>Überblick</span></a></li> <li id="menu-item-8640" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-8640"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/ueber-tron/translation/"><span>Translation</span></a></li> <li id="menu-item-6692" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-6692"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/ueber-tron/background-and-history/"><span>Background and History</span></a></li> <li id="menu-item-6691" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-6691"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/ueber-tron/ziele-und-kompetenz/"><span>Ziele und Kompetenz</span></a></li> <li id="menu-item-6690" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-6690"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/ueber-tron/personal-und-unternehmenskultur/"><span>Personal und Unternehmenskultur</span></a></li> <li id="menu-item-6689" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-6689"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/ueber-tron/management/"><span>Management</span></a></li> <li id="menu-item-6688" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-6688"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/ueber-tron/aufsichtsrat/"><span>Aufsichtsrat</span></a></li> </ul> </li> <li id="menu-item-34" class="menu-item menu-item-type-post_type menu-item-object-page current-menu-ancestor current_page_ancestor menu-item-has-children menu-item-34"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/forschung/"><span>Forschung</span></a> <ul class="sub-menu"> <li id="menu-item-6774" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-6774"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/forschung/"><span>Überblick</span></a></li> <li id="menu-item-6770" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-6770"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/forschung/fokus/"><span>Fokus</span></a></li> <li id="menu-item-6769" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-6769"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/forschung/biomarker/"><span>Biomarker</span></a></li> <li id="menu-item-6771" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-6771"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/forschung/immuntherapie/"><span>Immuntherapie</span></a></li> <li id="menu-item-6772" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-6772"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/forschung/praeklinische-modelle/"><span>Präklinische Modelle</span></a></li> <li id="menu-item-9295" class="menu-item menu-item-type-post_type menu-item-object-page current-menu-ancestor current-menu-parent current_page_parent current_page_ancestor menu-item-has-children menu-item-9295"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/2021-2/"><span>Publikationen</span></a> <ul class="sub-menu"> <li id="menu-item-9296" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-9296"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/2021-2/"><span>2021</span></a></li> <li id="menu-item-8999" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-8999"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/2020-2/"><span>2020</span></a></li> <li id="menu-item-8791" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-8791"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/2019-2/"><span>2019</span></a></li> <li id="menu-item-8461" class="menu-item menu-item-type-post_type menu-item-object-page current-menu-item page_item page-item-8451 current_page_item menu-item-8461"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/2018-2/" aria-current="page"><span>2018</span></a></li> <li id="menu-item-7853" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-7853"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/2017-2/"><span>2017</span></a></li> <li id="menu-item-6823" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-6823"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/2016-2/"><span>2016</span></a></li> <li id="menu-item-6822" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-6822"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/2015-2/"><span>2015</span></a></li> <li id="menu-item-6821" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-6821"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/2014-2/"><span>2014</span></a></li> <li id="menu-item-6820" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-6820"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/publications/2013-2/"><span>2013</span></a></li> <li id="menu-item-6819" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-6819"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/publications/2012-2/"><span>2012</span></a></li> <li id="menu-item-6818" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-6818"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/publications/2011-2/"><span>2011</span></a></li> <li id="menu-item-6817" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-6817"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/publications/2010-2/"><span>2010</span></a></li> </ul> </li> <li id="menu-item-6938" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-6938"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/forschung/phd-program/"><span>PhD Program</span></a></li> </ul> </li> <li id="menu-item-33" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-has-children menu-item-33"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/tron-abteilungen/"><span>Einrichtungen</span></a> <ul class="sub-menu"> <li id="menu-item-7116" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-7116"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/tron-abteilungen/"><span>Überblick</span></a></li> <li id="menu-item-6794" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-6794"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/tron-abteilungen/bioinformatics/"><span>Bioinformatics</span></a></li> <li id="menu-item-6797" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-6797"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/tron-abteilungen/genomics/"><span>Genomics</span></a></li> <li id="menu-item-6795" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-6795"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/tron-abteilungen/fe-in-vivo-imaging/"><span>In Vivo Imaging</span></a></li> <li id="menu-item-6799" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-6799"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/tron-abteilungen/fe-serodiscovery/"><span>Serodiscovery</span></a></li> <li id="menu-item-8414" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-8414"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/tron-abteilungen/validation/"><span>Validation</span></a></li> <li id="menu-item-6801" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-6801"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/tron-abteilungen/fe-vectors/"><span>Vectors</span></a></li> </ul> </li> <li id="menu-item-8363" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-8363"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/karriere/"><span>Karriere im TRON</span></a></li> <li id="menu-item-31" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-31"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/news/"><span>News</span></a></li> <li id="menu-item-7357" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-has-children menu-item-7357"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/veranstaltungen/"><span>Veranstaltungen</span></a> <ul class="sub-menu"> <li id="menu-item-7358" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-7358"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/veranstaltungen/"><span>Überblick</span></a></li> <li id="menu-item-7244" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-7244"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/veranstaltungen/translationale-onkologie/"><span>Seminare in Translationaler Onkologie</span></a></li> <li id="menu-item-7366" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-7366"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/veranstaltungen/rudern-gegen-krebs/"><span>Rudern gegen Krebs</span></a></li> <li id="menu-item-7365" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-7365"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/veranstaltungen/firmenlauf/"><span>Mainzer Firmenlauf</span></a></li> <li id="menu-item-7524" class="menu-item menu-item-type-post_type menu-item-object-page menu-item-7524"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/veranstaltungen/mainzer-wissenschaftsallianz-2016/"><span>Mainzer Wissenschaftsallianz</span></a></li> </ul> </li> </ul></div> <div class="side-nav"> <ul class="nav-search"> <li> <a class="search-contents" href="#"></a> <ul> <li> <div class="widget widget_search"><div class="search_main"> <form method="get" class="searchform" action="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/"> <input type="text" class="field s" name="s" value="Search..." onfocus="if (this.value == 'Search...') {this.value = '';}" onblur="if (this.value == '') {this.value = 'Search...';}"/> <button type="submit" class="fa fa-search submit" name="submit" value="Search"></button> </form> <div class="fix"></div> </div></div> </li> </ul> </li> </ul> </div> </section> <a href="#top" class="nav-close"> <i class="fa fa-times"></i> <span>Return to Content</span> </a> </nav> </div> </header> </div>  <div id="content" class="col-full"> <div id="main-sidebar-container">  <section id="main"> <article class="post-8451 page type-page status-publish has-post-thumbnail hentry category-publications"> <header> <h1 class="title entry-title">2018</h1> </header> <section class="entry"> <div class="vc_row wpb_row vc_row-fluid vc_column-gap-15 mpc-row"><div class="wpb_column vc_column_container vc_col-sm-9 mpc-column" data-column-id="mpc_column-47605591579ba5c"><div class="vc_column-inner"><div class="wpb_wrapper"> <div class="wpb_text_column wpb_content_element "> <div class="wpb_wrapper"> <p>Vormehr M, Reinhard K, Blatnik R, Josef K, Beck JD, Salomon N, Suchan M, Selmi A, Vascotto F, Zerweck J, Wenschuh H, Diken M, Kreiter S, Türeci Ö, Riemer AB, Sahin U. (2018) <strong>A non-functional neoepitope specific CD8+ T-cell response induced by tumor derived antigen exposure </strong><em><strong>in vivo</strong></em>. <em>Oncoimmunology</em>. 8(3):1553478.</p> <p><a href="https://web.archive.org/web/20210320060823/https://doi.org/10.1080/2162402X.2018.1553478">DOI</a>; <a href="https://web.archive.org/web/20210320060823/https://www.ncbi.nlm.nih.gov/pubmed/30723585">PMID</a></p> <div class="su-spoiler su-spoiler-style-default su-spoiler-icon-plus su-spoiler-closed" data-scroll-offset="0" data-anchor-in-url="no"><div class="su-spoiler-title" tabindex="0" role="button"><span class="su-spoiler-icon"></span>Show abstract</div><div class="su-spoiler-content su-u-clearfix su-u-trim">  Cancer-associated mutations, mostly single nucleotide variations, can act as neoepitopes and prime targets for effective anti-cancer T-cell immunity. T cells recognizing cancer mutations are critical for the clinical activity of immune checkpoint blockade (ICB) and they are potent vaccine antigens. High frequencies of mutation-specific T cells are rarely spontaneously induced. Hence, therapies that broaden the tumor specific T-cell response are of interest. Here, we analyzed neoepitope-specific CD8<sup>+</sup> T-cell responses mounted either spontaneously or after immunotherapy regimens, which induce local tumor inflammation and cell death, in mice bearing tumors of the widely used colon carcinoma cell line CT26. A comprehensive immune reactivity screening of 2474 peptides covering 628 transcribed CT26 point mutations was conducted. All tested treatment regimens were found to induce a single significant CD8<sup>+</sup> T-cell response against a non-synonymous D733A point mutation in the Smc3 gene. Surprisingly, even though Smc3 D733A turned out to be the immune-dominant neoepitope in CT26 tumor bearing mice, neither T cells specific for this neoepitope nor their T cell receptors (TCRs) were able to recognize or lyse tumor cells. Moreover, vaccination with the D733A neoepitope did not result in anti-tumoral activity despite induction of specific T cells. This is to our knowledge the first report that neoepitope specific CD8<sup>+</sup> T cells primed by tumor-released antigen exposure <em>in vivo</em> can be functionally irrelevant. </div></div> <p> </p> <p>Hilf N, Kuttruff-Coqui S, Frenzel K, Bukur V, Stevanović S, Gouttefangeas C, Platten M, Tabatabai G, Dutoit V, van der Burg SH, Thor Straten P, Martínez-Ricarte F, Ponsati B, Okada H, Lassen U, Admon A, Ottensmeier CH, Ulges A, Kreiter S, von Deimling A, Skardelly M, Migliorini D, Kroep JR, Idorn M, Rodon J, Piró J, Poulsen HS, Shraibman B, McCann K, Mendrzyk R, Löwer M, Stieglbauer M, Britten CM, Capper D, Welters MJP, Sahuquillo J, Kiesel K, Derhovanessian E, Rusch E, Bunse L, Song C, Heesch S, Wagner C, Kemmer-Brück A, Ludwig J, Castle JC, Schoor O, Tadmor AD, Green E, Fritsche J, Meyer M, Pawlowski N, Dorner S, Hoffgaard F, Rössler B, Maurer D, Weinschenk T, Reinhardt C, Huber C, Rammensee HG, Singh-Jasuja H, Sahin U, Dietrich PY, Wick W. (2018) <strong>Actively personalized vaccination trial for newly diagnosed glioblastoma</strong>. <em>Nature</em>. 565(7738):240-245.</p> <p><a href="https://web.archive.org/web/20210320060823/https://doi.org/10.1038/s41586-018-0810-y">DOI</a>; <a href="https://web.archive.org/web/20210320060823/https://www.ncbi.nlm.nih.gov/pubmed/30568303">PMID</a></p> <div class="su-spoiler su-spoiler-style-default su-spoiler-icon-plus su-spoiler-closed" data-scroll-offset="0" data-anchor-in-url="no"><div class="su-spoiler-title" tabindex="0" role="button"><span class="su-spoiler-icon"></span>Show abstract</div><div class="su-spoiler-content su-u-clearfix su-u-trim">  Patients with glioblastoma currently do not sufficiently benefit from recent breakthroughs in cancer treatment that use checkpoint inhibitors<sup><a href="https://web.archive.org/web/20210320060823/https://www.nature.com/articles/s41586-018-0810-y#ref-CR1">1</a>,<a href="https://web.archive.org/web/20210320060823/https://www.nature.com/articles/s41586-018-0810-y#ref-CR2">2</a></sup>. For treatments using checkpoint inhibitors to be successful, a high mutational load and responses to neoepitopes are thought to be essential<sup><a href="https://web.archive.org/web/20210320060823/https://www.nature.com/articles/s41586-018-0810-y#ref-CR3">3</a></sup>. There is limited intratumoural infiltration of immune cells<sup><a href="https://web.archive.org/web/20210320060823/https://www.nature.com/articles/s41586-018-0810-y#ref-CR4">4</a></sup> in glioblastoma and these tumours contain only 30–50 non-synonymous mutations<sup><a href="https://web.archive.org/web/20210320060823/https://www.nature.com/articles/s41586-018-0810-y#ref-CR5">5</a></sup>. Exploitation of the full repertoire of tumour antigens—that is, both unmutated antigens and neoepitopes—may offer more effective immunotherapies, especially for tumours with a low mutational load. Here, in the phase I trial GAPVAC-101 of the Glioma Actively Personalized Vaccine Consortium (GAPVAC), we integrated highly individualized vaccinations with both types of tumour antigens into standard care to optimally exploit the limited target space for patients with newly diagnosed glioblastoma. Fifteen patients with glioblastomas positive for human leukocyte antigen (HLA)-A*02:01 or HLA-A*24:02 were treated with a vaccine (APVAC1) derived from a premanufactured library of unmutated antigens followed by treatment with APVAC2, which preferentially targeted neoepitopes. Personalization was based on mutations and analyses of the transcriptomes and immunopeptidomes of the individual tumours. The GAPVAC approach was feasible and vaccines that had poly-ICLC (polyriboinosinic-polyribocytidylic acid-poly-L-lysine carboxymethylcellulose) and granulocyte–macrophage colony-stimulating factor as adjuvants displayed favourable safety and strong immunogenicity. Unmutated APVAC1 antigens elicited sustained responses of central memory CD8<sup>+</sup> T cells. APVAC2 induced predominantly CD4<sup>+</sup> T cell responses of T helper 1 type against predicted neoepitopes. </div></div> <p> </p> <p>Fiore A, Ugel S, Sanctis FD, Sandri S, Fracasso G, Trovato R, Sartoris S, Solito S, Mandruzzato S, Vascotto F, Hippen KL, Mondanelli G, Grohmann U, Piro G, Carbone C, Melisi D, Lawlor RT, Scarpa A, Lamolinara A, Iezzi M, Fassan M, Bicciato S, Blazar BR, Sahin U, Murray PJ, Bronte V. (2018) <strong>Induction of immunosuppressive functions and NF-kB by FLIP in monocytes</strong>. <em>Nat Commun</em>. 9:5193.</p> <p><a href="https://web.archive.org/web/20210320060823/https://doi.org/10.1038/s41467-018-07654-4">DOI</a>; <a href="https://web.archive.org/web/20210320060823/https://www.ncbi.nlm.nih.gov/pubmed/30518925">PMID</a></p> <div class="su-spoiler su-spoiler-style-default su-spoiler-icon-plus su-spoiler-closed" data-scroll-offset="0" data-anchor-in-url="no"><div class="su-spoiler-title" tabindex="0" role="button"><span class="su-spoiler-icon"></span>Show abstract</div><div class="su-spoiler-content su-u-clearfix su-u-trim">  Immunosuppression is a hallmark of tumor progression, and treatments that inhibit or deplete monocytic myeloid-derived suppressive cells could promote anti-tumor immunity. c-FLIP is a central regulator of caspase-8-mediated apoptosis and necroptosis. Here we show that low-dose cytotoxic chemotherapy agents cause apoptosis linked to c-FLIP down-regulation selectively in monocytes. Enforced expression of c-FLIP or viral FLIP rescues monocytes from cytotoxicity and concurrently induces potent immunosuppressive activity, in T cell cultures and in vivo models of tumor progression and immunotherapy. FLIP-transduced human blood monocytes can suppress graft versus host disease. Neither expression of FLIP in granulocytes nor expression of other anti-apoptotic genes in monocytes conferred immunosuppression, suggesting that FLIP effects on immunosuppression are specific to monocytic lineage and distinct from death inhibition. Mechanistically, FLIP controls a broad transcriptional program, partially by NF-κB activation. Therefore, modulation of FLIP in monocytes offers a means to elicit or block immunosuppressive myeloid cells. </div></div> <p> </p> <p>Sun Q, Barz M, De Geest BG, Diken M, Hennink WE, Kiessling F, Lammers T, Shi Y. (2018) <strong>Nanomedicine and macroscale materials in immuno-oncology</strong>. <em>Chem Soc Rev</em>. 48(1):351-381.</p> <p><a href="https://web.archive.org/web/20210320060823/https://pubs.rsc.org/en/Content/ArticleLanding/2018/CS/C8CS00473K#!divAbstract">DOI</a>; <a href="https://web.archive.org/web/20210320060823/https://www.ncbi.nlm.nih.gov/pubmed/?term=PMID%3A+30465669">PMID</a></p> <div class="su-spoiler su-spoiler-style-default su-spoiler-icon-plus su-spoiler-closed" data-scroll-offset="0" data-anchor-in-url="no"><div class="su-spoiler-title" tabindex="0" role="button"><span class="su-spoiler-icon"></span>Show abstract</div><div class="su-spoiler-content su-u-clearfix su-u-trim">  Immunotherapy is revolutionizing the treatment of cancer. It can achieve unprecedented responses in advanced-stage patients, including complete cures and long-term survival. However, immunotherapy also has limitations, such as its relatively low response rates and the development of severe side effects. These drawbacks are gradually being overcome by improving our understanding of the immune system, as well as by establishing combination regimens in which immunotherapy is combined with other treatment modalities. In addition to this, in recent years, progress made in chemistry, nanotechnology and materials science has started to impact immuno-oncology, resulting in more effective and less toxic immunotherapy interventions. In this context, multiple different nanomedicine formulations and macroscale materials have been shown to be able to boost anti-cancer immunity and the efficacy of immunomodulatory drugs. We here review nanotechnological and materials chemistry efforts related to endogenous and exogenous vaccination, to the engineering of antigen-presenting cells and T cells, and to the modulation of the tumor microenvironment. We also discuss limitations, current trends and future directions. Together, the insights provided and the evidence obtained indicate that there is a bright future ahead for engineering nanomedicines and macroscale materials for immuno-oncology applications. </div></div> <p> </p> <p>Pastor F, Berraondo P, Etxeberria I, Frederick J, Sahin U, Gilboa E and Melero I. (2018) <strong>An RNA toolbox for cancer immunotherapy</strong>. <em>Nat Rev</em>. <em>Drug Discov</em>. 17(10):751-767.</p> <p><a href="https://web.archive.org/web/20210320060823/https://www.nature.com/articles/nrd.2018.132">DOI</a>; <a href="https://web.archive.org/web/20210320060823/https://www.ncbi.nlm.nih.gov/pubmed/?term=PMID%3A+30190565">PMID</a></p> <div class="su-spoiler su-spoiler-style-default su-spoiler-icon-plus su-spoiler-closed" data-scroll-offset="0" data-anchor-in-url="no"><div class="su-spoiler-title" tabindex="0" role="button"><span class="su-spoiler-icon"></span>Show abstract</div><div class="su-spoiler-content su-u-clearfix su-u-trim">  Cancer immunotherapy has revolutionized oncology practice. However, current protein and cell therapy tools used in cancer immunotherapy are far from perfect, and there is room for improvement regarding their efficacy and safety. RNA-based structures have diverse functions, ranging from gene expression and gene regulation to pro-inflammatory effects and the ability to specifically bind different molecules. These functions make them versatile tools that may advance cancer vaccines and immunomodulation, surpassing existing approaches. These technologies should not be considered as competitors of current immunotherapies but as partners in synergistic combinations and as a clear opportunity to reach more efficient and personalized results. RNA and RNA derivatives can be exploited therapeutically as a platform to encode protein sequences, provide innate pro-inflammatory signals to the immune system (such as those denoting viral infection), control the expression of other RNAs (including key immunosuppressive factors) post-transcriptionally and conform structural scaffoldings binding proteins that control immune cells by modifying their function. Nascent RNA immunotherapeutics include RNA vaccines encoding cancer neoantigens, mRNAs encoding immunomodulatory factors, viral RNA analogues, interference RNAs and protein-binding RNA aptamers. These approaches are already in early clinical development with promising safety and efficacy results. </div></div> <p> </p> <p>Pektor S, Hilscher L, Walzer KC, Miederer I, Bausbacher N, Loquai C, Schreckenberger M, Sahin U, Diken M, Miederer M. (2018) <strong>In vivo imaging of the immune response upon systemic RNA cancer vaccination by FDG-PET. </strong>EJNMMI Res. 8(1):80.</p> <p><a href="https://web.archive.org/web/20210320060823/https://ejnmmires.springeropen.com/articles/10.1186/s13550-018-0435-z">DOI</a>; <a href="https://web.archive.org/web/20210320060823/https://www.ncbi.nlm.nih.gov/pubmed/30112704">PMID</a></p> <div class="su-spoiler su-spoiler-style-default su-spoiler-icon-plus su-spoiler-closed" data-scroll-offset="0" data-anchor-in-url="no"><div class="su-spoiler-title" tabindex="0" role="button"><span class="su-spoiler-icon"></span>Show abstract</div><div class="su-spoiler-content su-u-clearfix su-u-trim">  <strong>BACKGROUND:</strong> [<sup>18</sup>F]Fluoro-2-deoxy-2-d-glucose positron emission tomography (FDG-PET) is commonly used in the clinic for diagnosis of cancer and for follow-up of therapy outcome. Additional to the well-established value in tumor imaging, it bears potential to depict immune processes in modern immunotherapies. T cells enhance their glucose consumption upon activation and are crucial effectors for the success of such novel therapies. In this study, we analyzed the T cell immunity in spleen after antigen-specific stimulation of T cells via highly innovative RNA-based vaccines using FDG-PET/MRI. For this purpose, we employed systemic administration of RNA-lipoplexes encoding the endogenous antigen of Moloney murine leukemia virus (gp70) which have been previously shown to induce potent innate as well as adaptive immune mechanisms for cancer immunotherapy. Feasibility of clinical imaging of increased splenic FDG uptake was demonstrated in a melanoma patient participating in a clinical phase 1 trial of a tetravalent RNA-lipoplex cancer vaccine. <p><strong>RESULTS:</strong> We observed exclusive increase of glucose uptake in spleen compared to other organs thanks to liposome-mediated RNA targeting to this immune-relevant organ. In vivo and ex vivo FDG uptake analysis in the spleen of vaccinated mice correlated well with antigen-specific T cell activation. Moreover, the use of an irrelevant (antigen non-specific) RNA also resulted in enhanced FDG uptake early after vaccination through the activation of several other splenic cell populations. The glucose uptake was also dependent on the dose of RNA administered in line with the activation and frequencies of proliferating antigen-specific T cells as well as the general activation pattern of splenic cell populations.</p> <p><strong>CONCLUSIONS:</strong> Our preclinical results show rapid and transient vaccination-induced increase of FDG uptake within the spleen reflecting immune activation preceding T cell proliferation. FDG-PET/CT in patients is also capable to image this immune activation resulting in a new potential application of FDG-PET/CT to image immune processes in new immunological therapies.</p></div></div> <p> </p> <p>Türeci Ö, Löwer M, Schrörs B, Lang M, Tadmor A, Sahin U. (2018) <strong>Challenges towards the realization of individualized cancer vaccines</strong>. <em>Nat Biomed</em> <em>Eng</em>.  July 30;19:747.</p> <p><a href="https://web.archive.org/web/20210320060823/https://doi.org/10.1038/s41551-018-0266-2">DOI</a></p> <div class="su-spoiler su-spoiler-style-default su-spoiler-icon-plus su-spoiler-closed" data-scroll-offset="0" data-anchor-in-url="no"><div class="su-spoiler-title" tabindex="0" role="button"><span class="su-spoiler-icon"></span>Show abstract</div><div class="su-spoiler-content su-u-clearfix su-u-trim"> Bringing truly personalized cancer vaccination with tumour neoantigens to the clinic will require overcoming the challenges of optimized vaccine design, manufacturing and affordability, and identification of the most suitable clinical setting. </div></div> <p> </p> <p>Boegel S, Bukur T, Castle JC, Sahin U (2018) <strong>In Silico Typing of Classical and Non-classical HLA Alleles from Standard RNA-Seq Reads</strong> <em><span class="jrnl" title="Methods in molecular biology (Clifton, N.J.)">Methods Mol Biol</span></em>. 2018;1802:177-191.</p> <p><a href="https://web.archive.org/web/20210320060823/https://doi.org/10.1007/978-1-4939-8546-3_12">DOI</a>; <a href="https://web.archive.org/web/20210320060823/https://www.ncbi.nlm.nih.gov/pubmed/29858809">PMID</a></p> <div class="su-spoiler su-spoiler-style-default su-spoiler-icon-plus su-spoiler-closed" data-scroll-offset="0" data-anchor-in-url="no"><div class="su-spoiler-title" tabindex="0" role="button"><span class="su-spoiler-icon"></span>Show abstract</div><div class="su-spoiler-content su-u-clearfix su-u-trim"> Next-Generation Sequencing (NGS) enables the rapid generation of billions of short nucleic acid sequence fragments (i.e., “sequencing reads”). Especially, the adoption of gene expression profiling using whole transcriptome sequencing (i.e., “RNA-Seq”) has been rapid. Here, we describe an in silico method, seq2HLA, that takes standard RNA-Seq reads as input and determines a sample’s (classical and non-classical) HLA class I and class II types as well as HLA expression. We demonstrate the application of seq2HLA using publicly available RNA-Seq data from the Burkitt’s lymphoma cell line DAUDI and the choriocarcinoma cell line JEG-3. </div></div> <p> </p> <p>Schumacher J, Bacic T, Staritzbichler R, Daneschdar M, Klamp T, Arnold P, Jägle S, Türeci Ö, Markl J, Sahin U (2018) <strong>Enhanced stability of a chimeric hepatitis B core antigen virus-like-particle (HBcAg-VLP) by a C-terminal linker-hexahistidine-peptide.</strong> <em>J Nanobiotechnology</em>. Apr 13;16(1):39.</p> <p><a href="https://web.archive.org/web/20210320060823/http://10.1186/s12951-018-0363-0">DOI</a>; <a href="https://web.archive.org/web/20210320060823/https://www.ncbi.nlm.nih.gov/pubmed/29653575">PMID</a></p> <div class="su-spoiler su-spoiler-style-default su-spoiler-icon-plus su-spoiler-closed" data-scroll-offset="0" data-anchor-in-url="no"><div class="su-spoiler-title" tabindex="0" role="button"><span class="su-spoiler-icon"></span>Show abstract</div><div class="su-spoiler-content su-u-clearfix su-u-trim"> <strong>BACKGROUND</strong>: Virus-like-particles (VLPs) are attractive nanoparticulate scaffolds for broad applications in material/biological sciences and medicine. Prior their functionalization, specific adaptations have to be carried out. These adjustments frequently lead to disordered particles, but the particle integrity is an essential factor for the VLP suitability. Therefore, major requirements for particle stabilization exist. The objective of this study was to evaluate novel stabilizing elements for functionalized chimeric hepatitis B virus core antigen virus-like particles (HBcAg-VLP), with beneficial characteristics for vaccine development, imaging or delivery. <p><strong>RESULTS</strong>:<br> The effects of a carboxy-terminal polyhistidine-peptide and an intradimer disulfide-bridge on the stability of preclinically approved chimeric HBcAg-VLPs were assessed. We purified recombinant chimeric HBcAg-VLPs bearing different modified C-termini and compared their physical and chemical particle stability by quantitative protein-biochemical and biophysical techniques. We observed lower chemical resistance of T = 3- compared to T = 4-VLP (triangulation number) capsids and profound impairment of accessibility of hexahistidine-peptides in assembled VLPs. Histidines attached to the C-terminus were associated with superior mechanical and/or chemical particle stability depending on the number of histidine moieties. A molecular modeling approach based on cryo-electron microscopy and biolayer interferometry revealed the underlying structural mechanism for the strengthening of the integrity of VLPs. Interactions triggering capsid stabilization occur on a highly conserved residue on the basis of HBcAg-monomers as well as on hexahistidine-peptides of adjacent monomers. This new stabilization mechanism appears to mimic an evolutionary conserved stabilization concept for hepadnavirus core proteins.</p> <p><strong>CONCLUSIONS</strong>:<br> These findings establish the genetically simply transferable C-terminal polyhistidine-peptide as a general stabilizing element for chimeric HBcAg-VLPs to increase their suitability.</p></div></div> <p> </p> <p>Bidmon N, Kind S, Welters MJP, Joseph-Pietras D, Laske K, Maurer D, Hadrup SR, Schreibelt G, Rae R, Sahin U, Gouttefangeas C, Britten CM, van der Burg SH (2018) <strong>Development of an RNA-based kit for easy generation of TCR-engineered lymphocytes to control T-cell assay performance. </strong><em>J Immunol Methods.</em>  pii: S0022-1759(18)30025-5</p> <p><a href="https://web.archive.org/web/20210320060823/http://10.1016/j.jim.2018.04.007">DOI</a>; <a href="https://web.archive.org/web/20210320060823/https://www.ncbi.nlm.nih.gov/pubmed/29684430">PMID</a></p> <div class="su-spoiler su-spoiler-style-default su-spoiler-icon-plus su-spoiler-closed" data-scroll-offset="0" data-anchor-in-url="no"><div class="su-spoiler-title" tabindex="0" role="button"><span class="su-spoiler-icon"></span>Show abstract</div><div class="su-spoiler-content su-u-clearfix su-u-trim">Cell-based assays to monitor antigen-specific T-cell responses are characterized by their high complexity and should be conducted under controlled conditions to lower multiple possible sources of assay variation. However, the lack of standard reagents makes it difficult to directly compare results generated in one lab over time and across institutions. Therefore TCR-engineered reference samples (TERS) that contain a defined number of antigen-specific T cells and continuously deliver stable results are urgently needed. We successfully established a simple and robust TERS technology that constitutes a useful tool to overcome this issue for commonly used T-cell immuno-assays. To enable users to generate large-scale TERS, on-site using the most commonly used electroporation (EP) devices, an RNA-based kit approach, providing stable TCR mRNA and an optimized manufacturing protocol were established. In preparation for the release of this immuno-control kit, we established optimal EP conditions on six devices and initiated an extended RNA stability study. Furthermore, we coordinated on-site production of TERS with 4 participants. Finally, a proficiency panel was organized to test the unsupervised production of TERS at different laboratories using the kit approach. The results obtained show the feasibility and robustness of the kit approach for versatile in-house production of cellular control samples.</div></div> <p> </p> <p>Boegel S, Löwer M, Bukur T, Sorn P, Castle J, Sahin U. (2018) <strong>HLA and proteasome expression body map.</strong> <em>BMC Medical Genomics</em>. 11(1):36.</p> <p><a href="https://web.archive.org/web/20210320060823/https://bmcmedgenomics.biomedcentral.com/articles/10.1186/s12920-018-0354-x">DOI</a>; <a href="https://web.archive.org/web/20210320060823/https://www.ncbi.nlm.nih.gov/pubmed/29587858">PMID</a></p> <div class="su-spoiler su-spoiler-style-default su-spoiler-icon-plus su-spoiler-closed" data-scroll-offset="0" data-anchor-in-url="no"><div class="su-spoiler-title" tabindex="0" role="button"><span class="su-spoiler-icon"></span>Show abstract</div><div class="su-spoiler-content su-u-clearfix su-u-trim"><strong>Background</strong>: The presentation of HLA peptide complexes to T cells is a highly regulated and tissue specific process involving multiple transcriptionally controlled cellular components. The extensive polymorphism of HLA genes and the complex composition of the proteasome make it difficult to map their expression profiles across tissues.<br> <strong>Methods</strong>: Here we applied a tailored gene quantification pipeline to 4323 publicly available RNA-Seq datasets representing 55 normal tissues and cell types to examine expression profiles of (classical and non-classical) HLA class I, class II and proteasomal genes.<br> <strong>Results</strong>: We generated the first comprehensive expression atlas of antigen presenting-related genes across 56 normal tissues and cell types, including immune cells, pancreatic islets, platelets and hematopoietic stem cells. We found a surprisingly heterogeneous HLA expression pattern with up to 100-fold difference in intra-tissue median HLA abundances. Cells of the immune system and lymphatic organs expressed the highest levels of classical HLA class I (HLA-A,-B,-C), class II (HLA-DQA1,-DQB1,-DPA1,-DPB1,-DRA,-DRB1) and non-classical HLA class I (HLA-E,-F) molecules, whereas retina, brain, muscle, megakaryocytes and erythroblasts showed the lowest abundance. In contrast, we identified a distinct and highly tissue-restricted expression pattern of the non-classical class I gene HLA-G in placenta, pancreatic islets, pituitary gland and testis. While the constitutive proteasome showed relatively constant expression across all tissues, we found the immunoproteasome to be enriched in lymphatic organs and almost absent in immune privileged tissues.<br> <strong>Conclusions</strong>: Here, we not only provide a reference catalog of tissue and cell type specific HLA expression, but also highlight extremely variable expression of the basic components of antigen processing and presentation in different cell types. Our findings indicate that low expression of classical HLA class I molecules together with lack of immunoproteasome components as well as upregulation of HLA-G may be of key relevance to maintain tolerance in immune privileged tissues.</div></div> <p> </p> <p>Shen L, Tenzer S, Storck W, Hobernik D, Raker VK, Fischer K, Decker S, Dzionek A, Krauthäuser S, Diken M, Nikolaev A, Maxeiner J, Schuster P, Kappel C, Verschoor A, Schild H, Grabbe S, Bros M (2018) <strong>Protein corona-mediated targeting of nanocarriers to B cells allows redirection of allergic immune responses.</strong> <em>J Allergy Clin Immunol. </em>142(5):1558-1570.</p> <p><a href="https://web.archive.org/web/20210320060823/https://doi.org/10.1016/j.jaci.2017.08.049">DOI</a>; <a href="https://web.archive.org/web/20210320060823/https://www.ncbi.nlm.nih.gov/pubmed/?term=29382591">PMID</a></p> <div class="su-spoiler su-spoiler-style-default su-spoiler-icon-plus su-spoiler-closed" data-scroll-offset="0" data-anchor-in-url="no"><div class="su-spoiler-title" tabindex="0" role="button"><span class="su-spoiler-icon"></span>Show abstract</div><div class="su-spoiler-content su-u-clearfix su-u-trim"><strong>Background</strong>: Nanoparticle (NP)–based vaccines are attractive immunotherapy tools because of their capability to codeliver antigen and adjuvant to antigen-presenting cells. Their cellular distribution and serum protein interaction (“protein corona”) after systemic administration and their effect on the functional properties of NPs is poorly understood.<br> <strong>Objectives</strong>: We analyzed the relevance of the protein corona on cell type–selective uptake of dextran-coated NPs and determined the outcome of vaccination with NPs that codeliver antigen and adjuvant in disease models of allergy.<br> <strong>Methods</strong>: The role of protein corona constituents for cellular binding/uptake of dextran-coated ferrous nanoparticles (DEX-NPs) was analyzed both in vitro and in vivo. DEX-NPs conjugated with the model antigen ovalbumin (OVA) and immunostimulatory CpG-rich oligodeoxynucleotides were administered to monitor the induction of cellular and humoral immune responses. Therapeutic effects of this DEX-NP vaccine in mouse models of OVA-induced anaphylaxisand allergic asthma were assessed.<br> <strong>Results</strong>: DEX-NPs triggered lectin-induced complement activation, yielding deposition of activated complement factor 3 on the DEX-NP surface. In the spleen DEX-NPs targeted predominantly B cells through complement receptors 1 and 2. The DEX-NP vaccine elicited much stronger OVA-specific IgG2a production than coadministered soluble OVA plus CpG oligodeoxynucleotides. B-cell binding of the DEX-NP vaccine was critical for IgG2aproduction. Treatment of OVA-sensitized mice with the DEX-NP vaccine prevented induction of anaphylactic shock and allergic asthma accompanied by IgE inhibition.<br> <strong>Conclusions</strong>: Opsonization of lectin-coated NPs by activated complement components results in selective B-cell targeting. The intrinsic B-cell targeting property of lectin-coated NPs can be exploited for treatment of allergic immune responses</div></div> <p> </p> <p>Sahin U, Türeci Ö. (2018) <strong>Personalized vaccines for cancer immunotherapy</strong>. <em>Science</em>.  Mar 23; 359(6382):1355-1360.</p> <p><a href="https://web.archive.org/web/20210320060823/https://doi.org/10.1126/science.aar7112">DOI</a>; <a href="https://web.archive.org/web/20210320060823/https://www.ncbi.nlm.nih.gov/pubmed/29567706">PMID</a></p> <div class="su-spoiler su-spoiler-style-default su-spoiler-icon-plus su-spoiler-closed" data-scroll-offset="0" data-anchor-in-url="no"><div class="su-spoiler-title" tabindex="0" role="button"><span class="su-spoiler-icon"></span>Show abstract</div><div class="su-spoiler-content su-u-clearfix su-u-trim">Cancer is characterized by an accumulation of genetic alterations. Somatic mutations can generate cancer-specific neoepitopes that are recognized by autologous T cells as foreign and constitute ideal cancer vaccine targets. Every tumor has its own unique composition of mutations, with only a small fraction shared between patients. Technological advances in genomics, data science, and cancer immunotherapy now enable the rapid mapping of the mutations within a genome, rational selection of vaccine targets, and on-demand production of a therapy customized to a patient’s individual tumor. First-in-human clinical trials of personalized cancer vaccines have shown the feasibility, safety, and immunotherapeutic activity of targeting individual tumor mutation signatures. With vaccination development being promoted by emerging innovations of the digital age, vaccinating a patient with individual tumor mutations may become the first truly personalized treatment for cancer.</div></div> <p> </p> <p>Diken M, Chu KK, Brodsky AN. (2018) <strong>Translating Science into Survival: Report on the Third International Cancer Immunotherapy Conference</strong>. <em>Cancer Immunol Res</em>. 2018 Jan;6(1):10-13. doi: 10.1158/2326-6066.CIR-17-0656.</p> <p><a href="https://web.archive.org/web/20210320060823/https://doi.org/10.1158/2326-6066.CIR-17-0656">DOI</a>; <a href="https://web.archive.org/web/20210320060823/https://www.ncbi.nlm.nih.gov/pubmed/29298782">PMID</a></p> <div class="su-spoiler su-spoiler-style-default su-spoiler-icon-plus su-spoiler-closed" data-scroll-offset="0" data-anchor-in-url="no"><div class="su-spoiler-title" tabindex="0" role="button"><span class="su-spoiler-icon"></span>Show abstract</div><div class="su-spoiler-content su-u-clearfix su-u-trim">On September 6 to 9, 2017, in Mainz, Germany, the Third International Cancer Immunotherapy Conference was hosted jointly by the Cancer Research Institute, the Association for Cancer Immunotherapy, the European Academy of Tumor Immunology, and the American Association for Cancer Research. For the third straight year, more than 1,400 people attended the four-day event, which covered the latest advances in cancer immunology and immunotherapy. This report provides an overview of the main topics discussed.</div></div> </div> </div> </div></div></div><div class="wpb_column vc_column_container vc_col-sm-3 mpc-column"><div class="vc_column-inner"><div class="wpb_wrapper"> <div class="vc_grid-container-wrapper vc_clearfix"> <div class="vc_grid-container vc_clearfix wpb_content_element vc_basic_grid" data-initial-loading-animation="fadeIn" data-vc-grid-settings="{"page_id":8451,"style":"all","action":"vc_get_vc_grid_data","shortcode_id":"1516780861471-483aff06-d626-10","tag":"vc_basic_grid"}" data-vc-request="https://tron-mainz.de/de/wp-admin/admin-ajax.php" data-vc-post-id="8451" data-vc-public-nonce="660a59436e"> <style data-type="vc_shortcodes-custom-css">.vc_custom_1484316837418{background-color: #164679 !important;background-position: center !important;background-repeat: no-repeat !important;background-size: cover !important;}.vc_custom_1484317192348{background-color: #be3026 !important;}.vc_custom_1486920202167{margin-bottom: 0px !important;}.vc_custom_1486920211292{margin-bottom: 0px !important;}</style><div class="vc_grid vc_row vc_grid-gutter-15px vc_pageable-wrapper vc_hook_hover" data-vc-pageable-content="true"><div class="vc_pageable-slide-wrapper vc_clearfix" data-vc-grid-content="true"><div class="vc_grid-item vc_clearfix vc_col-sm-12"><div class="vc_grid-item-mini vc_clearfix "><div class="vc_gitem-animated-block vc_gitem-animate vc_gitem-animate-none" data-vc-animation="none"><div class="vc_gitem-zone vc_gitem-zone-a vc_custom_1484316837418 vc-gitem-zone-height-mode-auto vc-gitem-zone-height-mode-auto-16-9 vc_gitem-is-link"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/2021-2/" title="2021" class="vc_gitem-link vc-zone-link"></a><div class="vc_gitem-zone-mini"><div class="vc_gitem_row vc_row vc_gitem-row-position-middle"><div class="vc_col-sm-12 vc_gitem-col vc_gitem-col-align-"><div class="vc_custom_heading sidegrid vc_custom_1486920202167 vc_gitem-post-data vc_gitem-post-data-source-post_title"><h3 style="color: #ffffff;text-align: center;font-family:Lato;font-weight:700;font-style:normal"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/2021-2/" class="vc_gitem-link" title="2021">2021</a></h3></div></div></div></div></div><div class="vc_gitem-zone vc_gitem-zone-b vc_custom_1484317192348 vc-gitem-zone-height-mode-auto vc_gitem-is-link"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/2021-2/" title="2021" class="vc_gitem-link vc-zone-link"></a><div class="vc_gitem-zone-mini"><div class="vc_gitem_row vc_row vc_gitem-row-position-middle"><div class="vc_col-sm-12 vc_gitem-col vc_gitem-col-align-"><div class="vc_custom_heading sidegrid vc_custom_1486920211292 vc_gitem-post-data vc_gitem-post-data-source-post_title"><h3 style="color: #ffffff;text-align: center;font-family:Lato;font-weight:700;font-style:normal"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/2021-2/" class="vc_gitem-link" title="2021">2021</a></h3></div></div></div></div></div></div></div><div class="vc_clearfix"></div></div><div class="vc_grid-item vc_clearfix vc_col-sm-12"><div class="vc_grid-item-mini vc_clearfix "><div class="vc_gitem-animated-block vc_gitem-animate vc_gitem-animate-none" data-vc-animation="none"><div class="vc_gitem-zone vc_gitem-zone-a vc_custom_1484316837418 vc-gitem-zone-height-mode-auto vc-gitem-zone-height-mode-auto-16-9 vc_gitem-is-link"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/2020-2/" title="2020" class="vc_gitem-link vc-zone-link"></a><div class="vc_gitem-zone-mini"><div class="vc_gitem_row vc_row vc_gitem-row-position-middle"><div class="vc_col-sm-12 vc_gitem-col vc_gitem-col-align-"><div class="vc_custom_heading sidegrid vc_custom_1486920202167 vc_gitem-post-data vc_gitem-post-data-source-post_title"><h3 style="color: #ffffff;text-align: center;font-family:Lato;font-weight:700;font-style:normal"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/2020-2/" class="vc_gitem-link" title="2020">2020</a></h3></div></div></div></div></div><div class="vc_gitem-zone vc_gitem-zone-b vc_custom_1484317192348 vc-gitem-zone-height-mode-auto vc_gitem-is-link"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/2020-2/" title="2020" class="vc_gitem-link vc-zone-link"></a><div class="vc_gitem-zone-mini"><div class="vc_gitem_row vc_row vc_gitem-row-position-middle"><div class="vc_col-sm-12 vc_gitem-col vc_gitem-col-align-"><div class="vc_custom_heading sidegrid vc_custom_1486920211292 vc_gitem-post-data vc_gitem-post-data-source-post_title"><h3 style="color: #ffffff;text-align: center;font-family:Lato;font-weight:700;font-style:normal"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/2020-2/" class="vc_gitem-link" title="2020">2020</a></h3></div></div></div></div></div></div></div><div class="vc_clearfix"></div></div><div class="vc_grid-item vc_clearfix vc_col-sm-12"><div class="vc_grid-item-mini vc_clearfix "><div class="vc_gitem-animated-block vc_gitem-animate vc_gitem-animate-none" data-vc-animation="none"><div class="vc_gitem-zone vc_gitem-zone-a vc_custom_1484316837418 vc-gitem-zone-height-mode-auto vc-gitem-zone-height-mode-auto-16-9 vc_gitem-is-link"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/2019-2/" title="2019" class="vc_gitem-link vc-zone-link"></a><div class="vc_gitem-zone-mini"><div class="vc_gitem_row vc_row vc_gitem-row-position-middle"><div class="vc_col-sm-12 vc_gitem-col vc_gitem-col-align-"><div class="vc_custom_heading sidegrid vc_custom_1486920202167 vc_gitem-post-data vc_gitem-post-data-source-post_title"><h3 style="color: #ffffff;text-align: center;font-family:Lato;font-weight:700;font-style:normal"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/2019-2/" class="vc_gitem-link" title="2019">2019</a></h3></div></div></div></div></div><div class="vc_gitem-zone vc_gitem-zone-b vc_custom_1484317192348 vc-gitem-zone-height-mode-auto vc_gitem-is-link"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/2019-2/" title="2019" class="vc_gitem-link vc-zone-link"></a><div class="vc_gitem-zone-mini"><div class="vc_gitem_row vc_row vc_gitem-row-position-middle"><div class="vc_col-sm-12 vc_gitem-col vc_gitem-col-align-"><div class="vc_custom_heading sidegrid vc_custom_1486920211292 vc_gitem-post-data vc_gitem-post-data-source-post_title"><h3 style="color: #ffffff;text-align: center;font-family:Lato;font-weight:700;font-style:normal"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/2019-2/" class="vc_gitem-link" title="2019">2019</a></h3></div></div></div></div></div></div></div><div class="vc_clearfix"></div></div><div class="vc_grid-item vc_clearfix vc_col-sm-12"><div class="vc_grid-item-mini vc_clearfix "><div class="vc_gitem-animated-block vc_gitem-animate vc_gitem-animate-none" data-vc-animation="none"><div class="vc_gitem-zone vc_gitem-zone-a vc_custom_1484316837418 vc-gitem-zone-height-mode-auto vc-gitem-zone-height-mode-auto-16-9 vc_gitem-is-link"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/2017-2/" title="2017" class="vc_gitem-link vc-zone-link"></a><div class="vc_gitem-zone-mini"><div class="vc_gitem_row vc_row vc_gitem-row-position-middle"><div class="vc_col-sm-12 vc_gitem-col vc_gitem-col-align-"><div class="vc_custom_heading sidegrid vc_custom_1486920202167 vc_gitem-post-data vc_gitem-post-data-source-post_title"><h3 style="color: #ffffff;text-align: center;font-family:Lato;font-weight:700;font-style:normal"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/2017-2/" class="vc_gitem-link" title="2017">2017</a></h3></div></div></div></div></div><div class="vc_gitem-zone vc_gitem-zone-b vc_custom_1484317192348 vc-gitem-zone-height-mode-auto vc_gitem-is-link"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/2017-2/" title="2017" class="vc_gitem-link vc-zone-link"></a><div class="vc_gitem-zone-mini"><div class="vc_gitem_row vc_row vc_gitem-row-position-middle"><div class="vc_col-sm-12 vc_gitem-col vc_gitem-col-align-"><div class="vc_custom_heading sidegrid vc_custom_1486920211292 vc_gitem-post-data vc_gitem-post-data-source-post_title"><h3 style="color: #ffffff;text-align: center;font-family:Lato;font-weight:700;font-style:normal"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/2017-2/" class="vc_gitem-link" title="2017">2017</a></h3></div></div></div></div></div></div></div><div class="vc_clearfix"></div></div><div class="vc_grid-item vc_clearfix vc_col-sm-12"><div class="vc_grid-item-mini vc_clearfix "><div class="vc_gitem-animated-block vc_gitem-animate vc_gitem-animate-none" data-vc-animation="none"><div class="vc_gitem-zone vc_gitem-zone-a vc_custom_1484316837418 vc-gitem-zone-height-mode-auto vc-gitem-zone-height-mode-auto-16-9 vc_gitem-is-link"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/2016-2/" title="2016" class="vc_gitem-link vc-zone-link"></a><div class="vc_gitem-zone-mini"><div class="vc_gitem_row vc_row vc_gitem-row-position-middle"><div class="vc_col-sm-12 vc_gitem-col vc_gitem-col-align-"><div class="vc_custom_heading sidegrid vc_custom_1486920202167 vc_gitem-post-data vc_gitem-post-data-source-post_title"><h3 style="color: #ffffff;text-align: center;font-family:Lato;font-weight:700;font-style:normal"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/2016-2/" class="vc_gitem-link" title="2016">2016</a></h3></div></div></div></div></div><div class="vc_gitem-zone vc_gitem-zone-b vc_custom_1484317192348 vc-gitem-zone-height-mode-auto vc_gitem-is-link"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/2016-2/" title="2016" class="vc_gitem-link vc-zone-link"></a><div class="vc_gitem-zone-mini"><div class="vc_gitem_row vc_row vc_gitem-row-position-middle"><div class="vc_col-sm-12 vc_gitem-col vc_gitem-col-align-"><div class="vc_custom_heading sidegrid vc_custom_1486920211292 vc_gitem-post-data vc_gitem-post-data-source-post_title"><h3 style="color: #ffffff;text-align: center;font-family:Lato;font-weight:700;font-style:normal"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/2016-2/" class="vc_gitem-link" title="2016">2016</a></h3></div></div></div></div></div></div></div><div class="vc_clearfix"></div></div><div class="vc_grid-item vc_clearfix vc_col-sm-12"><div class="vc_grid-item-mini vc_clearfix "><div class="vc_gitem-animated-block vc_gitem-animate vc_gitem-animate-none" data-vc-animation="none"><div class="vc_gitem-zone vc_gitem-zone-a vc_custom_1484316837418 vc-gitem-zone-height-mode-auto vc-gitem-zone-height-mode-auto-16-9 vc_gitem-is-link"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/2015-2/" title="2015" class="vc_gitem-link vc-zone-link"></a><div class="vc_gitem-zone-mini"><div class="vc_gitem_row vc_row vc_gitem-row-position-middle"><div class="vc_col-sm-12 vc_gitem-col vc_gitem-col-align-"><div class="vc_custom_heading sidegrid vc_custom_1486920202167 vc_gitem-post-data vc_gitem-post-data-source-post_title"><h3 style="color: #ffffff;text-align: center;font-family:Lato;font-weight:700;font-style:normal"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/2015-2/" class="vc_gitem-link" title="2015">2015</a></h3></div></div></div></div></div><div class="vc_gitem-zone vc_gitem-zone-b vc_custom_1484317192348 vc-gitem-zone-height-mode-auto vc_gitem-is-link"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/2015-2/" title="2015" class="vc_gitem-link vc-zone-link"></a><div class="vc_gitem-zone-mini"><div class="vc_gitem_row vc_row vc_gitem-row-position-middle"><div class="vc_col-sm-12 vc_gitem-col vc_gitem-col-align-"><div class="vc_custom_heading sidegrid vc_custom_1486920211292 vc_gitem-post-data vc_gitem-post-data-source-post_title"><h3 style="color: #ffffff;text-align: center;font-family:Lato;font-weight:700;font-style:normal"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/2015-2/" class="vc_gitem-link" title="2015">2015</a></h3></div></div></div></div></div></div></div><div class="vc_clearfix"></div></div><div class="vc_grid-item vc_clearfix vc_col-sm-12"><div class="vc_grid-item-mini vc_clearfix "><div class="vc_gitem-animated-block vc_gitem-animate vc_gitem-animate-none" data-vc-animation="none"><div class="vc_gitem-zone vc_gitem-zone-a vc_custom_1484316837418 vc-gitem-zone-height-mode-auto vc-gitem-zone-height-mode-auto-16-9 vc_gitem-is-link"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/2014-2/" title="2014" class="vc_gitem-link vc-zone-link"></a><div class="vc_gitem-zone-mini"><div class="vc_gitem_row vc_row vc_gitem-row-position-middle"><div class="vc_col-sm-12 vc_gitem-col vc_gitem-col-align-"><div class="vc_custom_heading sidegrid vc_custom_1486920202167 vc_gitem-post-data vc_gitem-post-data-source-post_title"><h3 style="color: #ffffff;text-align: center;font-family:Lato;font-weight:700;font-style:normal"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/2014-2/" class="vc_gitem-link" title="2014">2014</a></h3></div></div></div></div></div><div class="vc_gitem-zone vc_gitem-zone-b vc_custom_1484317192348 vc-gitem-zone-height-mode-auto vc_gitem-is-link"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/2014-2/" title="2014" class="vc_gitem-link vc-zone-link"></a><div class="vc_gitem-zone-mini"><div class="vc_gitem_row vc_row vc_gitem-row-position-middle"><div class="vc_col-sm-12 vc_gitem-col vc_gitem-col-align-"><div class="vc_custom_heading sidegrid vc_custom_1486920211292 vc_gitem-post-data vc_gitem-post-data-source-post_title"><h3 style="color: #ffffff;text-align: center;font-family:Lato;font-weight:700;font-style:normal"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/2014-2/" class="vc_gitem-link" title="2014">2014</a></h3></div></div></div></div></div></div></div><div class="vc_clearfix"></div></div><div class="vc_grid-item vc_clearfix vc_col-sm-12"><div class="vc_grid-item-mini vc_clearfix "><div class="vc_gitem-animated-block vc_gitem-animate vc_gitem-animate-none" data-vc-animation="none"><div class="vc_gitem-zone vc_gitem-zone-a vc_custom_1484316837418 vc-gitem-zone-height-mode-auto vc-gitem-zone-height-mode-auto-16-9 vc_gitem-is-link"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/publications/2013-2/" title="2013" class="vc_gitem-link vc-zone-link"></a><div class="vc_gitem-zone-mini"><div class="vc_gitem_row vc_row vc_gitem-row-position-middle"><div class="vc_col-sm-12 vc_gitem-col vc_gitem-col-align-"><div class="vc_custom_heading sidegrid vc_custom_1486920202167 vc_gitem-post-data vc_gitem-post-data-source-post_title"><h3 style="color: #ffffff;text-align: center;font-family:Lato;font-weight:700;font-style:normal"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/publications/2013-2/" class="vc_gitem-link" title="2013">2013</a></h3></div></div></div></div></div><div class="vc_gitem-zone vc_gitem-zone-b vc_custom_1484317192348 vc-gitem-zone-height-mode-auto vc_gitem-is-link"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/publications/2013-2/" title="2013" class="vc_gitem-link vc-zone-link"></a><div class="vc_gitem-zone-mini"><div class="vc_gitem_row vc_row vc_gitem-row-position-middle"><div class="vc_col-sm-12 vc_gitem-col vc_gitem-col-align-"><div class="vc_custom_heading sidegrid vc_custom_1486920211292 vc_gitem-post-data vc_gitem-post-data-source-post_title"><h3 style="color: #ffffff;text-align: center;font-family:Lato;font-weight:700;font-style:normal"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/publications/2013-2/" class="vc_gitem-link" title="2013">2013</a></h3></div></div></div></div></div></div></div><div class="vc_clearfix"></div></div><div class="vc_grid-item vc_clearfix vc_col-sm-12"><div class="vc_grid-item-mini vc_clearfix "><div class="vc_gitem-animated-block vc_gitem-animate vc_gitem-animate-none" data-vc-animation="none"><div class="vc_gitem-zone vc_gitem-zone-a vc_custom_1484316837418 vc-gitem-zone-height-mode-auto vc-gitem-zone-height-mode-auto-16-9 vc_gitem-is-link"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/publications/2012-2/" title="2012" class="vc_gitem-link vc-zone-link"></a><div class="vc_gitem-zone-mini"><div class="vc_gitem_row vc_row vc_gitem-row-position-middle"><div class="vc_col-sm-12 vc_gitem-col vc_gitem-col-align-"><div class="vc_custom_heading sidegrid vc_custom_1486920202167 vc_gitem-post-data vc_gitem-post-data-source-post_title"><h3 style="color: #ffffff;text-align: center;font-family:Lato;font-weight:700;font-style:normal"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/publications/2012-2/" class="vc_gitem-link" title="2012">2012</a></h3></div></div></div></div></div><div class="vc_gitem-zone vc_gitem-zone-b vc_custom_1484317192348 vc-gitem-zone-height-mode-auto vc_gitem-is-link"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/publications/2012-2/" title="2012" class="vc_gitem-link vc-zone-link"></a><div class="vc_gitem-zone-mini"><div class="vc_gitem_row vc_row vc_gitem-row-position-middle"><div class="vc_col-sm-12 vc_gitem-col vc_gitem-col-align-"><div class="vc_custom_heading sidegrid vc_custom_1486920211292 vc_gitem-post-data vc_gitem-post-data-source-post_title"><h3 style="color: #ffffff;text-align: center;font-family:Lato;font-weight:700;font-style:normal"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/publications/2012-2/" class="vc_gitem-link" title="2012">2012</a></h3></div></div></div></div></div></div></div><div class="vc_clearfix"></div></div><div class="vc_grid-item vc_clearfix vc_col-sm-12"><div class="vc_grid-item-mini vc_clearfix "><div class="vc_gitem-animated-block vc_gitem-animate vc_gitem-animate-none" data-vc-animation="none"><div class="vc_gitem-zone vc_gitem-zone-a vc_custom_1484316837418 vc-gitem-zone-height-mode-auto vc-gitem-zone-height-mode-auto-16-9 vc_gitem-is-link"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/publications/2011-2/" title="2011" class="vc_gitem-link vc-zone-link"></a><div class="vc_gitem-zone-mini"><div class="vc_gitem_row vc_row vc_gitem-row-position-middle"><div class="vc_col-sm-12 vc_gitem-col vc_gitem-col-align-"><div class="vc_custom_heading sidegrid vc_custom_1486920202167 vc_gitem-post-data vc_gitem-post-data-source-post_title"><h3 style="color: #ffffff;text-align: center;font-family:Lato;font-weight:700;font-style:normal"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/publications/2011-2/" class="vc_gitem-link" title="2011">2011</a></h3></div></div></div></div></div><div class="vc_gitem-zone vc_gitem-zone-b vc_custom_1484317192348 vc-gitem-zone-height-mode-auto vc_gitem-is-link"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/publications/2011-2/" title="2011" class="vc_gitem-link vc-zone-link"></a><div class="vc_gitem-zone-mini"><div class="vc_gitem_row vc_row vc_gitem-row-position-middle"><div class="vc_col-sm-12 vc_gitem-col vc_gitem-col-align-"><div class="vc_custom_heading sidegrid vc_custom_1486920211292 vc_gitem-post-data vc_gitem-post-data-source-post_title"><h3 style="color: #ffffff;text-align: center;font-family:Lato;font-weight:700;font-style:normal"><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/publications/2011-2/" class="vc_gitem-link" title="2011">2011</a></h3></div></div></div></div></div></div></div><div class="vc_clearfix"></div></div></div></div> </div> </div></div></div></div></div> </section> <div class="fix"></div> </article> </section> </div> </div>  <div id="footer-widgets-container"> <section id="footer-widgets" class="col-full col-4"> <div class="block footer-widget-1"> <div id="text-2" class="widget widget_text"> <div class="textwidget"><p><img src="https://web.archive.org/web/20210320060823im_/https://tron-mainz.de/wp-content/uploads/2016/10/wappen.jpg" alt="Gefördert vom Land Rheinland-Pfalz"/></p> </div> </div> </div> <div class="block footer-widget-2"> <div id="text-3" class="widget widget_text"> <div class="textwidget"><p><img src="https://web.archive.org/web/20210320060823im_/https://tron-mainz.de/wp-content/uploads/2016/10/bmbf-logo-de.jpg" alt="Gefördert vom Bundesministerium für Bildung und Forschung"/></p> </div> </div> </div> <div class="block footer-widget-3"> <div id="text-5" class="widget widget_text"> <div class="textwidget"><p><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/wp-content/uploads/sites/9/2020/08/DFG-logo_foerderung.jpg"><img loading="lazy" class="alignnone size-medium wp-image-9186" src="https://web.archive.org/web/20210320060823im_/https://tron-mainz.de/de/wp-content/uploads/sites/9/2020/08/DFG-logo_foerderung-300x85.jpg" alt="" width="300" height="85" srcset="https://web.archive.org/web/20210320060823im_/https://tron-mainz.de/de/wp-content/uploads/sites/9/2020/08/DFG-logo_foerderung-300x85.jpg 300w, https://web.archive.org/web/20210320060823im_/https://tron-mainz.de/de/wp-content/uploads/sites/9/2020/08/DFG-logo_foerderung-1024x290.jpg 1024w, https://web.archive.org/web/20210320060823im_/https://tron-mainz.de/de/wp-content/uploads/sites/9/2020/08/DFG-logo_foerderung-768x217.jpg 768w, https://web.archive.org/web/20210320060823im_/https://tron-mainz.de/de/wp-content/uploads/sites/9/2020/08/DFG-logo_foerderung.jpg 1057w" sizes="(max-width: 300px) 100vw, 300px"/></a></p> </div> </div> </div> <div class="block footer-widget-4"> <div id="text-7" class="widget widget_text"> <div class="textwidget"><p><a href="https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/wp-content/uploads/sites/9/2020/08/EC-logo_DE.jpg"><img loading="lazy" class="alignnone wp-image-9187" src="https://web.archive.org/web/20210320060823im_/https://tron-mainz.de/de/wp-content/uploads/sites/9/2020/08/EC-logo_DE-300x208.jpg" alt="" width="160" height="111" srcset="https://web.archive.org/web/20210320060823im_/https://tron-mainz.de/de/wp-content/uploads/sites/9/2020/08/EC-logo_DE-300x208.jpg 300w, https://web.archive.org/web/20210320060823im_/https://tron-mainz.de/de/wp-content/uploads/sites/9/2020/08/EC-logo_DE-1024x709.jpg 1024w, https://web.archive.org/web/20210320060823im_/https://tron-mainz.de/de/wp-content/uploads/sites/9/2020/08/EC-logo_DE-768x531.jpg 768w, https://web.archive.org/web/20210320060823im_/https://tron-mainz.de/de/wp-content/uploads/sites/9/2020/08/EC-logo_DE.jpg 1104w" sizes="(max-width: 160px) 100vw, 160px"/></a></p> </div> </div> </div> <div class="fix"></div> </section> </div>  <div id="footer-container"> <footer id="footer" class="col-full"> <div id="copyright" class="col-left"> <p><p>© 2021 TRON Mainz. All Rights Reserved</p></p> </div> <div id="credit" class="col-right"> <p><p class="footer-menu"><b><a href="/web/20210320060823/https://tron-mainz.de/de/datenschutz/">DATENSCHUTZ</a> | <a href="/web/20210320060823/https://tron-mainz.de/de/press-and-pr/">PRESSE UND PR</a> | <a href="/web/20210320060823/https://tron-mainz.de/de/karriere/">KARRIERE</a> | <a href="/web/20210320060823/https://tron-mainz.de/de/ausschreibungen/">AUSSCHREIBUNGEN</a> | <a href="/web/20210320060823/https://tron-mainz.de/de/kontakt/">KONTAKT</a> | <a href="/web/20210320060823/https://tron-mainz.de/de/glossary/glossary-a/">GLOSSARY</a> | <a href="/web/20210320060823/https://tron-mainz.de/de/impressum/">IMPRESSUM</a> | <a href="/web/20210320060823/https://tron-mainz.de/de/aebs/">AEBs</a></b></p></p> </div> </footer> </div> </div> </div> <div class="fix"></div> <script> window.addEventListener('sfsi_plus_functions_loaded', function() { if (typeof sfsi_plus_responsive_toggle == 'function') { sfsi_plus_responsive_toggle(0); // console.log('sfsi_plus_responsive_toggle'); } }) </script> <link rel="stylesheet" id="mpc_column-css-css" href="https://web.archive.org/web/20210320060823cs_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/mpc_massive_pack_col/shortcodes/mpc_column/css/mpc_column_css.css" type="text/css" media="all"/> <link rel="stylesheet" id="lightbox2-css" href="https://web.archive.org/web/20210320060823cs_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/js_composer/assets/lib/bower/lightbox2/dist/css/lightbox_min_css.css" type="text/css" media="all"/> <link rel="stylesheet" id="vc_pageable_owl-carousel-css-css" href="https://web.archive.org/web/20210320060823cs_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/js_composer/assets/lib/owl_carousel2_dist/assets/owl_min_css.css" type="text/css" media="all"/> <link rel="stylesheet" id="vc_animate-css-css" href="https://web.archive.org/web/20210320060823cs_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/js_composer/assets/lib/bower/animate_css/animate_min_css.css" type="text/css" media="all"/> <link rel="stylesheet" id="vc_google_fonts_lato100100italic300300italicregularitalic700700italic900900italic-css" href="https://web.archive.org/web/20210320060823cs_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/fonts.googleapis.com/css_family_Lato_3A100_2C147212186.css&subset=latin&ver=6.6.0" type="text/css" media="all"/> <link rel="stylesheet" id="su-icons-css" href="https://web.archive.org/web/20210320060823cs_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/shortcodes_ultimate/includes/css/icons_css.css" type="text/css" media="all"/> <link rel="stylesheet" id="su-shortcodes-css" href="https://web.archive.org/web/20210320060823cs_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/shortcodes_ultimate/includes/css/shortcodes_css.css" type="text/css" media="all"/> <script type="text/javascript" src="https://web.archive.org/web/20210320060823js_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/swift_box_wp/js/sb/swift_box_min_js.js" id="nb-core-js"></script> <script type="text/javascript" src="https://web.archive.org/web/20210320060823js_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_includes/js/jquery/ui/core_min_js.js" id="jquery-ui-core-js"></script> <script type="text/javascript" src="https://web.archive.org/web/20210320060823js_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/ultimate_social_media_plus/js/shuffle/modernizr_custom_min_js.js" id="SFSIPLUSjqueryModernizr-js"></script> <script type="text/javascript" id="SFSIPLUSCustomJs-js-extra"> /* <![CDATA[ */ var sfsi_plus_ajax_object = {"ajax_url":"https:\/\/web.archive.org\/web\/20210320060823\/https:\/\/tron-mainz.de\/de\/wp-admin\/admin-ajax.php","plugin_url":"https:\/\/web.archive.org\/web\/20210320060823\/https:\/\/tron-mainz.de\/de\/wp-content\/plugins\/ultimate-social-media-plus\/","rest_url":"https:\/\/web.archive.org\/web\/20210320060823\/https:\/\/tron-mainz.de\/de\/wp-json\/"}; var sfsi_plus_links = {"admin_url":"https:\/\/web.archive.org\/web\/20210320060823\/https:\/\/tron-mainz.de\/de\/wp-admin\/","plugin_dir_url":"https:\/\/web.archive.org\/web\/20210320060823\/https:\/\/tron-mainz.de\/de\/wp-content\/plugins\/ultimate-social-media-plus\/","rest_url":"https:\/\/web.archive.org\/web\/20210320060823\/https:\/\/tron-mainz.de\/de\/wp-json\/","pretty_perma":"yes"}; /* ]]> */ </script> <script type="text/javascript" src="https://web.archive.org/web/20210320060823js_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/ultimate_social_media_plus/js/custom_js.js" id="SFSIPLUSCustomJs-js"></script> <script type="text/javascript" id="mpc-massive-vendor-script-js-extra"> /* <![CDATA[ */ var _mpc_ajax = "https:\/\/web.archive.org\/web\/20210320060823\/https:\/\/tron-mainz.de\/de\/wp-admin\/admin-ajax.php"; var _mpc_animations = "0"; var _mpc_scroll_to_id = "1"; /* ]]> */ </script> <script type="text/javascript" src="https://web.archive.org/web/20210320060823js_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/mpc_massive_pack_col/assets/js/mpc_vendor_min_js.js" id="mpc-massive-vendor-script-js"></script> <script type="text/javascript" src="https://web.archive.org/web/20210320060823js_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/mpc_massive_pack_col/assets/js/mpc_main_min_js.js" id="mpc-massive-main-script-js"></script> <script type="text/javascript" src="https://web.archive.org/web/20210320060823js_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_includes/js/wp_embed_min_js.js" id="wp-embed-js"></script> <script type="text/javascript" src="https://web.archive.org/web/20210320060823js_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/js_composer/assets/js/dist/js_composer_front_min_js.js" id="wpb_composer_front_js-js"></script> <script type="text/javascript" src="https://web.archive.org/web/20210320060823js_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/mpc_massive_pack_col/shortcodes/mpc_column/js/mpc_column_min_js.js" id="mpc_column-js-js"></script> <script type="text/javascript" src="https://web.archive.org/web/20210320060823js_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/js_composer/assets/lib/bower/lightbox2/dist/js/lightbox_min_js.js" id="lightbox2-js"></script> <script type="text/javascript" src="https://web.archive.org/web/20210320060823js_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/js_composer/assets/lib/owl_carousel2_dist/owl_carousel_min_js.js" id="vc_pageable_owl-carousel-js"></script> <script type="text/javascript" src="https://web.archive.org/web/20210320060823js_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/js_composer/assets/lib/bower/imagesloaded/imagesloaded_pkgd_min_js.js" id="vc_grid-js-imagesloaded-js"></script> <script type="text/javascript" src="https://web.archive.org/web/20210320060823js_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_includes/js/underscore_min_js.js" id="underscore-js"></script> <script type="text/javascript" src="https://web.archive.org/web/20210320060823js_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/js_composer/assets/lib/vc_waypoints/vc_waypoints_min_js.js" id="vc_waypoints-js"></script> <script type="text/javascript" src="https://web.archive.org/web/20210320060823js_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/js_composer/assets/js/dist/vc_grid_min_js.js" id="vc_grid-js"></script> <script type="text/javascript" id="su-shortcodes-js-extra"> /* <![CDATA[ */ var SUShortcodesL10n = {"noPreview":"Dieser Shortcode funktioniert nicht in der Live-Vorschau. Bitte f\u00fcge ihn im Editor ein und sieh dir die Vorschau der Seite an.","magnificPopup":{"close":"Schlie\u00dfen (Esc)","loading":"Wird geladen...","prev":"Zur\u00fcck (linke Pfeiltaste)","next":"Vor (rechte Pfeiltaste)","counter":"%curr% von %total%","error":"Failed to load content. <a href=\"%url%\" target=\"_blank\"><u>Open link<\/u><\/a>"}}; /* ]]> */ </script> <script type="text/javascript" src="https://web.archive.org/web/20210320060823js_/https://tron-mainz.de/de/wp-content/gdprpatron/hts/tron-mainz.de/de/wp_content/plugins/shortcodes_ultimate/includes/js/shortcodes/index_js.js" id="su-shortcodes-js"></script>  <style type="text/css"> /* Ultimate Social Media Plus next to Logo .sfsi_plus_widget { position: absolute; left: 16em;}*/ header .sfsi_plus_widget {display: none;} header .widget .sfsi_plus_widget {display: inline-block;} .sfsiplus_norm_row.sfsi_plus_wDiv { text-align: left!important; position: relative!important; width: 100px!important; } #sfsi_holder {display: none;} /* Multilingual Press Translation - Move Widget Display */ .mlp-language-box ul li a { color: #ffffff; background-color: #000000; padding: 0.4em; text-transform: uppercase; border-radius: 100%; font-size: 0.9em; } .widget ul {list-style-type: none;} #mlp_widget-3, #sfsi-plus-widget-2 {float: right;} @media screen and (min-width: 747px) { .mlp-language-box {padding-top: 3px;} .header-widget .widget{margin-top: 0.3em;} } @media screen and (max-width: 746px) { .mlp-language-box {padding-top: 1px;} .header-widget .widget{margin-top: 0;} }</style>   <style type="text/css"> /* BLAUER HINTERGRUND DER GESCHLOSSENEN TEILE .vc_tta-color-black.vc_tta-style-modern .vc_tta-panel .vc_tta-panel-heading { border: none!important; background-color: #164679!important; text-transform: uppercase; } .vc_tta.vc_tta-style-modern .vc_tta-panel-heading { background-image: none!important; } .vc_tta-color-black.vc_tta-style-modern .vc_tta-panel .vc_tta-panel-heading:hover { background-color: #be3026!important; } .vc_tta-color-black.vc_tta-style-modern .vc_tta-panel.vc_active .vc_tta-panel-title>a { color: #be3026!important; background-color: #fff!important; } */ /* GRAUER HINTERGRUND DER GESCHLOSSENEN TEILE*/ .vc_tta-color-black.vc_tta-style-modern .vc_tta-panel .vc_tta-panel-heading { background-color: #f7f7f7!important; text-transform: uppercase; border: none!important; } .vc_tta-color-black.vc_tta-style-modern .vc_tta-panel .vc_tta-panel-title>a { color: #164679!important; } .vc_tta-color-black.vc_tta-style-modern .vc_tta-panel.vc_active .vc_tta-panel-title>a { color: #be3026!important; background-color: #fff; } .vc_tta-color-black.vc_tta-style-modern .vc_tta-controls-icon::after, .vc_tta-color-black.vc_tta-style-modern .vc_tta-controls-icon::before { border-color: #164679!important; } /*Same width as main content*/ .vc_tta.vc_general .vc_tta-panel-body { padding: 0!important; }</style>   <style type="text/css"> h3 { text-transform: uppercase; } .entry p { color: #555555!important; } .title { text-align: center; text-transform: uppercase; } .page-title, .post .title, .page .title { margin: 0 0 1.5em; } .wp3changerequest_change .title {font-size: 10px;}</style>   <style type="text/css"> .sidegrid a:link { color: #ffffff!important } .homegrid a:link { color: #ffffff!important }</style>   <style type="text/css"> #footer { padding-top: 0px; padding-bottom: 0px; } </style>   <style type="text/css"> #navigation ul.nav-search .searchform { background: #f3f3f3; } .menu-item {text-transform: uppercase;} .menu-item a {text-decoration: none;} /* Make some menu wider, so it fits German Words */ @media only screen and (min-width: 768px) { #navigation ul.nav li ul { width: 200px; } }</style>   <style type="text/css"> #top { width: 100%; left: 0em; } </style>  <script type="text/javascript"> nb_lightbox = true; nb_touchswipe = true; nb_min_news_h = 150; nb_min_news_w = 200; nb_min_horiz_w = 400; nb_read_more_txt = "..."; nb_fb_share_fix = "https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/wp-content/plugins/swift-box-wp/lcis_fb_img_fix.php"; nb_script_basepath = "https://web.archive.org/web/20210320060823/https://tron-mainz.de/de/wp-content/plugins/swift-box-wp/js/sb/"; nb_short_d_names = ["Sun", "Mon", "Tue", "Wed", "Thu", "Fri", "Sat"]; nb_full_d_names = ["Sunday", "Monday", "Tuesday", "Wednesday", "Thursday", "Friday", "Saturday"]; nb_short_m_names = ["Jan", "Feb", "Mar", "Apr", "May", "Jun", "Jul", "Aug", "Sep", "Oct", "Nov", "Dec"]; nb_full_m_names = ["January", "February", "March", "April", "May", "June", "July", "August", "September", "October", "November", "December"]; nb_elapsed_names = ["ago", "seconds", "minute", "minutes", "hour", "hours", "day", "days", "week", "weeks", "month", "months"]; if( typeof(lcnb_loaded_themes) == 'undefined' ) {lcnb_loaded_themes = new Array();} lcnb_loaded_themes.push('wpdt'); </script> <script type="text/javascript"> jQuery(document).ready(function ($) { //$( document ).ajaxStart(function() { //}); for (var i = 0; i < document.forms.length; ++i) { var form = document.forms[i]; if ($(form).attr("method") != "get") { $(form).append('<input type="hidden" name="LmFA-fMksg" value="eUEMdSDYQFuo4" />'); } if ($(form).attr("method") != "get") { $(form).append('<input type="hidden" name="UMYocDy" value="d3f1XeA" />'); } if ($(form).attr("method") != "get") { $(form).append('<input type="hidden" name="WpGXRi" value="hq8Y1fQb0JmujT" />'); } if ($(form).attr("method") != "get") { $(form).append('<input type="hidden" name="qKQtFObfWEk" value="q0U]S5eIh8Hv[G" />'); } } $(document).on('submit', 'form', function () { if ($(this).attr("method") != "get") { $(this).append('<input type="hidden" name="LmFA-fMksg" value="eUEMdSDYQFuo4" />'); } if ($(this).attr("method") != "get") { $(this).append('<input type="hidden" name="UMYocDy" value="d3f1XeA" />'); } if ($(this).attr("method") != "get") { $(this).append('<input type="hidden" name="WpGXRi" value="hq8Y1fQb0JmujT" />'); } if ($(this).attr("method") != "get") { $(this).append('<input type="hidden" name="qKQtFObfWEk" value="q0U]S5eIh8Hv[G" />'); } return true; }); jQuery.ajaxSetup({ beforeSend: function (e, data) { //console.log(Object.getOwnPropertyNames(data).sort()); //console.log(data.type); if (data.type !== 'POST') return; if (typeof data.data === 'object' && data.data !== null) { data.data.append("LmFA-fMksg", "eUEMdSDYQFuo4"); data.data.append("UMYocDy", "d3f1XeA"); data.data.append("WpGXRi", "hq8Y1fQb0JmujT"); data.data.append("qKQtFObfWEk", "q0U]S5eIh8Hv[G"); } else { data.data = data.data + '&LmFA-fMksg=eUEMdSDYQFuo4&UMYocDy=d3f1XeA&WpGXRi=hq8Y1fQb0JmujT&qKQtFObfWEk=q0U]S5eIh8Hv[G'; } } }); }); </script> </body> </html>