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expires=' + expires.toGMTString() + '; domain=.' + this.getMasterDomain() + ';'; }, /** * get the master domain based on business rules */ getMasterDomain : function () { // default the master domain var masterDomain = 'thermofisher.com'; // check for alternate domain portion of URL var regexMatch = document.location.hostname.match(/\.(thermofisher)\.(cn)/i); if (regexMatch !== null) { masterDomain = regexMatch[1] + '.' + regexMatch[2]; } return masterDomain; }, /** * Check if init has been initiated once * @type {Boolean} */ initted: false, /** * @return {Array} method returns an array of a given object's key properties */ init: function () { this.page.country = this.getCookie("CK_ISO_CODE") || 'us'; this.page.language = this.getCookie('CK_LANG_CODE') || 'en'; this.page.siteName = 'thermo'; //hard coded this.initMerchandisingData(); this.initUserData(); this.displayDigitalData(); this.formSubmitEvent(); }, //specific function to set type of the page // need specific function to keep page type separate from other page attributes setPageType: function (type) { this.setPageAttribute('type', type); 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max-width: 870px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"12765238-65ac-4e13-b3b4-8be838f535d2MainParsysimage_3965 .image-contain" + "," + "#cq-image-jsp-"+"12765238-65ac-4e13-b3b4-8be838f535d2MainParsysimage_3965 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div><div class="text parbase section"><h2>Protect your frozen cells with Gibco cryopreservation media</h2><p>Freezing down cells guards against unplanned mutations, senescence, and contamination. While unprotected cell freezing is ordinarily lethal, cell freezing with cryopreservation media helps protect cells for long-term viability as well as minimizes freezing injury for an intact cell supply on your schedule.</p><p>View protocols for <a href="/sg/en/home/references/gibco-cell-culture-basics/cell-culture-protocols/freezing-cells.html">cell freezing</a> and <a href="/sg/en/home/references/gibco-cell-culture-basics/cell-culture-protocols/thawing-cells.html">thawing</a> for more information.</p></div><div class="heading section"><h2> Freezing down cells in DMSO </h2></div><div class="text parbase section"><p>The use of a freezing medium should contain a cryoprotective agent like <a></a><a href="/order/catalog/product/J66650.AK">dimethyl sulfoxide (DMSO)</a> or glycerol. These agents reduce culture-medium freezing points and allow for slower cooling rates; greatly reducing the risk of ice crystal formation which can damage and kill untreated cells.</p><p>While each of the following Gibco freezing media are optimized for different cell types, they are all ready-to-use and contain 10% DMSO.</p></div><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#" href="#cryopreservationselectionguide"><span class="caret"></span> View cryopreservation media selection guide </a></div><div class="accordion-body collapse " id="cryopreservationselectionguide"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="text parbase section"><h3>Choose the right cryopreservation media for your cell type</h3><div class="table-component-wrapper"><table class="rte-table" id="table-1583380125"><tbody><tr><th width="22%"> </th><th width="26%"><a href="#synth-a-freeze">Synth-a-Freeze Cryopreservation Medium</a></th><th width="26%"><a href="#recovery-cell-freezing">Recovery Cell Culture Freezing Medium</a></th><th width="26%"><a href="#cryopreservation-kit">PSC Cryopreservation Kit</a></th></tr><tr><td><b>Tested cell types</b></td><td>Human keratinocytes, ESCs, MSCs, NSCs, other primary cell types</td><td>CHO-S, CHO-K1, HEK 293, Jurkat, NIH 3T3</td><td>Pluripotent stem cells, peripheral blood mononuclear cells</td></tr><tr><td><b>Not suitable for</b></td><td>Melanocytes</td><td style="text-align: center;">—</td><td style="text-align: center;">—</td></tr><tr><td><b>Chemical properties</b></td><td>Animal origin-free</td><td>Contains FBS</td><td>Xeno-free (Cryomedium)<br><br> Animal origin-free <a href="#revita-cell">(RevitaCell)</a></td></tr></tbody></table></div></div></div></div></div></div></div></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt2"><div class="parsys_column cq-colctrl-lt2-c0"><div container-id="cq-colctrl-lt2" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-12765238-65ac-4e13-b3b4-8be838f535d2MainParsysimage_c12b"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/image_c12b/foregroundimg.img.full.high.jpg/1729013094676.jpg' media="(min-width: 1px)"><img class='image-contain' srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/image_c12b/foregroundimg.img.full.high.jpg/1729013094676.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/image_c12b/foregroundimg.img.320.high.jpg/1729013094676.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/image_c12b/foregroundimg.img.480.high.jpg/1729013094676.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/image_c12b/foregroundimg.img.320.medium.jpg/1729013094676.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/image_c12b/foregroundimg.img.620.high.jpg/1729013094676.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/image_c12b/foregroundimg.img.320.low.jpg/1729013094676.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/image_c12b/foregroundimg.img.480.medium.jpg/1729013094676.jpg" alt='Recovery Cell Culture Freezing Medium product bottle' title='Recovery Freezing Medium'></picture><style></style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"12765238-65ac-4e13-b3b4-8be838f535d2MainParsysimage_c12b .image-contain" + "," + "#cq-image-jsp-"+"12765238-65ac-4e13-b3b4-8be838f535d2MainParsysimage_c12b .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div><div class="parsys_column cq-colctrl-lt2-c1"><div container-id="cq-colctrl-lt2" comp-id="B1"><div class="text parbase section"><h3><a name="recovery-cell-freezing" id="recovery-cell-freezing"></a><br> Recovery Cell Culture Freezing Medium</h3><p>A complete, ready-to-use freezing medium, the Recovery medium formulation avoids messy mixing. Using an optimized version of the classic DMEM, serum, and DMSO, Recovery cell freezing medium is ready to go when you are. When treated with Recovery medium, cells experience improved recovery and viability post-thaw.</p></div></div></div></div><div style="clear:both"></div><div class="accordion section"><div class="accordion" id="9b860c7063fe49138957416ddbb77df7" style='margin-bottom: 0;'><div class="parsys"><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#9b860c7063fe49138957416ddbb77df7" href="#recoverysectionguide"><span class="caret"></span> View Recovery Freezing Medium cell viability data </a></div><div class="accordion-body collapse " id="recoverysectionguide"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="heading section"><h3> Comparative analysis of Recovery Cell Culture Freezing Medium vs. currently available cryopreservation technologies </h3></div><div class="image parbase section"><div id="cq-image-jsp-12765238-65ac-4e13-b3b4-8be838f535d2MainParsysaccordion_2defitemsparaccordionitem_e66eitemParsysimage_103a"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/accordion_2def/itemspar/accordionitem_e66e/itemParsys/image_103a/foregroundimg.img.320.low.png/1729013094787.png' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/accordion_2def/itemspar/accordionitem_e66e/itemParsys/image_103a/foregroundimg.img.320.medium.png/1729013094787.png' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/accordion_2def/itemspar/accordionitem_e66e/itemParsys/image_103a/foregroundimg.img.480.medium.png/1729013094787.png' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/accordion_2def/itemspar/accordionitem_e66e/itemParsys/image_103a/foregroundimg.img.620.high.png/1729013094787.png' media="(min-width: 630px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/accordion_2def/itemspar/accordionitem_e66e/itemParsys/image_103a/foregroundimg.img.full.high.png/1729013094787.png' media="(min-width: 1025px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/accordion_2def/itemspar/accordionitem_e66e/itemParsys/image_103a/foregroundimg.img.full.high.png/1729013094787.png,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/accordion_2def/itemspar/accordionitem_e66e/itemParsys/image_103a/foregroundimg.img.320.high.png/1729013094787.png,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/accordion_2def/itemspar/accordionitem_e66e/itemParsys/image_103a/foregroundimg.img.480.high.png/1729013094787.png,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/accordion_2def/itemspar/accordionitem_e66e/itemParsys/image_103a/foregroundimg.img.320.medium.png/1729013094787.png,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/accordion_2def/itemspar/accordionitem_e66e/itemParsys/image_103a/foregroundimg.img.620.high.png/1729013094787.png,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/accordion_2def/itemspar/accordionitem_e66e/itemParsys/image_103a/foregroundimg.img.320.low.png/1729013094787.png,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/accordion_2def/itemspar/accordionitem_e66e/itemParsys/image_103a/foregroundimg.img.480.medium.png/1729013094787.png" alt='Bar chart of cell recovery percent for 4 different cell lines frozen in Recovery medium and 6 alternative media' title='Recovery medium performance data'></picture><style> #cq-image-jsp-12765238-65ac-4e13-b3b4-8be838f535d2MainParsysaccordion_2defitemsparaccordionitem_e66eitemParsysimage_103a img{ width:100%; max-width: 1162px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"12765238-65ac-4e13-b3b4-8be838f535d2MainParsysaccordion_2defitemsparaccordionitem_e66eitemParsysimage_103a .image-contain" + "," + "#cq-image-jsp-"+"12765238-65ac-4e13-b3b4-8be838f535d2MainParsysaccordion_2defitemsparaccordionitem_e66eitemParsysimage_103a .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div><div class="text parbase section"><p><b>Method:</b> For each of four different cell lines (i.e., CHO, MDCK, Swiss 3T3, HeLa), the same number of cells were frozen into each of seven different cryopreservation media. Upon thawing, each cell sample was diluted into the same volume of a growth medium and seeded in 12-well plates. The initial seeding densities were 2.3×10<sup>4</sup> cells/well for adherent cell lines, and 6.0×10<sup>5</sup> cells/well for non-adherent cell lines, based on cell counts determined at the cryopreservation step. </p><p>When the leading culture for a particular cell line had reached late log growth and none of the other cultures from that cell line had passed peak density, all cultures for that cell line were harvested and the data were analyzed. Thus, different cell lines were analyzed on different days: CHO, day 6; MDCK, day 6; Swiss 3T3, day 5; and HeLa, day 6. Cell recovery of replicate cultures from different cryopreservation media were normalized to Recovery Cell Culture Freezing Medium and plotted (average normalized recovery±standard error).</p><p><b>Result:</b> For cryopreservation of both adherent and suspension cell lines, Recovery freezing medium results in an average of 25% higher cell viability compared to other cell freezing media:</p><p><a class="cta-link" href="https://assets.thermofisher.com/TFS-Assets/BID/Flyers/recovery-cell-culture-freezing-medium-flyer.pdf" target="_blank">Flyer: Recovery freezing medium cell viability</a></p><p> </p></div></div></div></div></div></div></div><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#9b860c7063fe49138957416ddbb77df7" href="#culturefreezingmediumtable"><span class="caret"></span> Order Recovery Cell Culture Freezing Medium </a></div><div class="accordion-body collapse " id="culturefreezingmediumtable"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="productlist parbase section"><div id="skulist" style="display:none">12648010</div><div id='ltajax-7220042911621458294'></div><script type="text/javascript">(function() {$('#ltajax-7220042911621458294').load('/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/accordion_2def/itemspar/accordionitem_a799/itemParsys/productlist_943b.ajax.html?skulist=');})(); </script></div></div></div></div></div></div></div></div></div><script> $j(document).ready(function(){ $j('#9b860c7063fe49138957416ddbb77df7').on('hide', function(e) { $(e.target).find(".accordion-content").hide(); }); $j('#9b860c7063fe49138957416ddbb77df7').on('show', function(e) { $(e.target).find(".accordion-content").show(); }); }); </script></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt2"><div class="parsys_column cq-colctrl-lt2-c0"><div container-id="cq-colctrl-lt2" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-12765238-65ac-4e13-b3b4-8be838f535d2MainParsysimage_3d4f"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/image_3d4f/foregroundimg.img.full.high.jpg/1729013094874.jpg' media="(min-width: 1px)"><img class='image-contain' srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/image_3d4f/foregroundimg.img.full.high.jpg/1729013094874.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/image_3d4f/foregroundimg.img.320.high.jpg/1729013094874.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/image_3d4f/foregroundimg.img.480.high.jpg/1729013094874.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/image_3d4f/foregroundimg.img.320.medium.jpg/1729013094874.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/image_3d4f/foregroundimg.img.620.high.jpg/1729013094874.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/image_3d4f/foregroundimg.img.320.low.jpg/1729013094874.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/image_3d4f/foregroundimg.img.480.medium.jpg/1729013094874.jpg" alt='Synth-a-Freeze medium product bottle' title='Synth-a-Freeze Cryopreservation Medium'></picture><style></style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"12765238-65ac-4e13-b3b4-8be838f535d2MainParsysimage_3d4f .image-contain" + "," + "#cq-image-jsp-"+"12765238-65ac-4e13-b3b4-8be838f535d2MainParsysimage_3d4f .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div><div class="parsys_column cq-colctrl-lt2-c1"><div container-id="cq-colctrl-lt2" comp-id="B1"><div class="text parbase section"><h3><a name="synth-a-freeze" id="synth-a-freeze"></a>Synth-a-Freeze Cryopreservation Medium</h3><p>Formulated without antibiotics, antimycotics, hormones, growth factors, serum or proteins, Synth-a-Freeze medium is defined and ready-to-use. Synth-a-Freeze medium is buffered with HEPES and sodium bicarbonate and designed for use with primary mammalian cell types including human keratinocytes, embryonic stem cells, neural stem cells, and mesenchymal stem cells.</p><div class="alert alert-info"><p><b>NOTE:</b> Synth-a-Freeze medium is not appropriate for melanocytes.</p></div></div></div></div></div><div style="clear:both"></div><div class="accordion section"><div class="accordion" id="0b5c49081d044f729bfbc638fbffb891" style='margin-bottom: 0;'><div class="parsys"><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#0b5c49081d044f729bfbc638fbffb891" href="#cryopreservationmediumtable"><span class="caret"></span> Order Synth-a-Freeze Cryopreservation Medium </a></div><div class="accordion-body collapse " id="cryopreservationmediumtable"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="productlist parbase section"><div id="skulist" style="display:none">A1254201,A1371301</div><div id='ltajax-7259860610986505473'></div><script type="text/javascript">(function() {$('#ltajax-7259860610986505473').load('/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/accordion_6a36/itemspar/accordionitem_a9a/itemParsys/productlist_75ab.ajax.html?skulist=');})(); </script></div></div></div></div></div></div></div></div></div><script> $j(document).ready(function(){ $j('#0b5c49081d044f729bfbc638fbffb891').on('hide', function(e) { $(e.target).find(".accordion-content").hide(); }); $j('#0b5c49081d044f729bfbc638fbffb891').on('show', function(e) { $(e.target).find(".accordion-content").show(); }); }); </script></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt2"><div class="parsys_column cq-colctrl-lt2-c0"><div container-id="cq-colctrl-lt2" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-12765238-65ac-4e13-b3b4-8be838f535d2MainParsysimage_4c81"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/image_4c81/foregroundimg.img.full.high.jpg/1729013094988.jpg' media="(min-width: 1px)"><img class='image-auto' srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/image_4c81/foregroundimg.img.full.high.jpg/1729013094988.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/image_4c81/foregroundimg.img.320.high.jpg/1729013094988.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/image_4c81/foregroundimg.img.480.high.jpg/1729013094988.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/image_4c81/foregroundimg.img.320.medium.jpg/1729013094988.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/image_4c81/foregroundimg.img.620.high.jpg/1729013094988.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/image_4c81/foregroundimg.img.320.low.jpg/1729013094988.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/image_4c81/foregroundimg.img.480.medium.jpg/1729013094988.jpg" alt='PSC Cryopreservation Kit product bottles' title='PSC Cryopreservation Kit'></picture><style> #cq-image-jsp-12765238-65ac-4e13-b3b4-8be838f535d2MainParsysimage_4c81 img{ width:100%; max-width: 100%; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"12765238-65ac-4e13-b3b4-8be838f535d2MainParsysimage_4c81 .image-contain" + "," + "#cq-image-jsp-"+"12765238-65ac-4e13-b3b4-8be838f535d2MainParsysimage_4c81 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div><div class="parsys_column cq-colctrl-lt2-c1"><div container-id="cq-colctrl-lt2" comp-id="B1"><div class="text parbase section"><h3><a name="cryopreservation-kit" id="cryopreservation-kit"></a>PSC Cryopreservation Kit</h3><p>The PSC Cryopreservation Kit contains ready-to-use, xeno-free cryopreservation medium as well as a bottle of <a href="/order/catalog/product/A2644501">RevitaCell Supplement (100X)</a>. Together, these reagents minimize cell viability loss and unwanted cell differentiation while maximizing post-thaw recovery. Applications include pluripotent stem cells cultured in media systems, such as Essential 8 and StemPro hESC SFM, as well as other commercially available formulations. In addition, this kit can also be used to cryopreserve and recover peripheral blood mononuclear cells (PBMCs).</p></div></div></div></div><div style="clear:both"></div><div class="accordion section"><div class="accordion" id="d50f72e0bf2a4ea8987c8662bb959628" style='margin-bottom: 0;'><div class="parsys"><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#d50f72e0bf2a4ea8987c8662bb959628" href="#cryopreservationkittable"><span class="caret"></span> Order PSC Cryopreservation Kit </a></div><div class="accordion-body collapse " id="cryopreservationkittable"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="productlist parbase section"><div id="skulist" style="display:none">A2644601,A4239301</div><div id='ltajax-8919501755617148045'></div><script type="text/javascript">(function() {$('#ltajax-8919501755617148045').load('/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/accordion_9147/itemspar/accordionitem_f9b4/itemParsys/productlist_849a.ajax.html?skulist=');})(); </script></div></div></div></div></div></div></div></div></div><script> $j(document).ready(function(){ $j('#d50f72e0bf2a4ea8987c8662bb959628').on('hide', function(e) { $(e.target).find(".accordion-content").hide(); }); $j('#d50f72e0bf2a4ea8987c8662bb959628').on('show', function(e) { $(e.target).find(".accordion-content").show(); }); }); </script></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt2"><div class="parsys_column cq-colctrl-lt2-c0"><div container-id="cq-colctrl-lt2" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-12765238-65ac-4e13-b3b4-8be838f535d2MainParsysimage_722c"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/image_722c/foregroundimg.img.full.high.jpg/1729013095109.jpg' media="(min-width: 1px)"><img class='image-contain' srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/image_722c/foregroundimg.img.full.high.jpg/1729013095109.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/image_722c/foregroundimg.img.320.high.jpg/1729013095109.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/image_722c/foregroundimg.img.480.high.jpg/1729013095109.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/image_722c/foregroundimg.img.320.medium.jpg/1729013095109.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/image_722c/foregroundimg.img.620.high.jpg/1729013095109.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/image_722c/foregroundimg.img.320.low.jpg/1729013095109.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/cell-freezing-media/jcr:content/MainParsys/image_722c/foregroundimg.img.480.medium.jpg/1729013095109.jpg" alt='RevitaCell supplement product bottle' title='RevitaCell supplement'></picture><style></style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"12765238-65ac-4e13-b3b4-8be838f535d2MainParsysimage_722c .image-contain" + "," + "#cq-image-jsp-"+"12765238-65ac-4e13-b3b4-8be838f535d2MainParsysimage_722c .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div><div class="parsys_column cq-colctrl-lt2-c1"><div container-id="cq-colctrl-lt2" comp-id="B1"><div class="text parbase section"><h3><a name="revita-cell" id="revita-cell"></a>RevitaCell supplement</h3><p>Containing Rho-associated protein kinase (ROCK) inhibitors and antioxidant properties, the RevitaCell supplement typically recovers pluripotent stem cells at 40,000 viable cells/cm<sup>2</sup>, and significantly reduces the average post-thaw recovery time of most cryopreservation solutions. In addition to pluripotent stem cells, RevitaCell supplements can also be utilized with primary cells including neurons, human epidermal keratinocytes, and human corneal epithelial cells.</p></div></div></div></div><div style="clear:both"></div><div class="accordion section"><div class="accordion" id="8d31aae33c4747cf8bf7f97e8764e6b6" style='margin-bottom: 0;'><div class="parsys"><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#8d31aae33c4747cf8bf7f97e8764e6b6" href="#revitacellsupplementtable"><span class="caret"></span> Order RevitaCell supplement </a></div><div class="accordion-body collapse " id="revitacellsupplementtable"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="productlist parbase section"><div id="skulist" style="display:none">A2644501, A4238401</div><div id='ltajax-9103622269555961666'></div><script 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products</a></li><li><a href="/sg/en/home/life-science/cell-culture/mammalian-cell-culture/recombinant-proteins.html">Recombinant proteins</a></li></ul></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="heading section"><div class="h2"> Support </div></div></div><div container-id="cq-colctrl-lt0" comp-id="B2"><div class="text parbase section"><p><a href="/sg/en/home/technical-resources/technical-reference-library/cell-culture-transfection-support-centers.html"><b>Cell Culture & Transfection Support Center</b></a><br> Find technical support recommendations for your cell culture and transfection workflows, including tips for experimental setup and in-depth troubleshooting help.</p><p><a href="/sg/en/home/technical-resources/contact-us.html" target="_blank"><b>Need technical support?</b></a> Contact our expert team for technical and application support of Laboratory Products.</p></div></div></div></div><div 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