CINXE.COM

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group_no_search_section_wrap clearfix" about="/products/flow-cytometry-workflow-solutions" typeof="sioc:Item foaf:Document"> <header> <span property="dc:title" content="Flow Cytometry Workflow Solutions " class="rdf-meta element-hidden"></span><span property="sioc:num_replies" content="0" datatype="xsd:integer" class="rdf-meta element-hidden"></span> </header> <div class="required-fields group-hero-image-wrapper field-group-html-element"><div class="field field-name-field-hero-image field-type-et-image-field field-label-hidden"><div class="field-items"><div class="field-item even"><div class=""> <div class="hero-image-text-content"> <div class="hero-image-text-content-inner"> </div> </div> <img loading="lazy" src="https://resources.rndsystems.com/images/banners/flow-cy-workflow-bannerv2.jpg" class="et-image-image" alt="Flow Cytometry Workflow Solutions" /> </div> </div></div></div></div><div class="product-category-search-wrapper col-xs-12 col-sm-8 col-md-9 col-lg-9 col-xl-9"><h2 class="group-search-title-wrapper field-group-html-element"><div class="field field-name-field-search-title field-type-text field-label-hidden"><div class="field-items"><div class="field-item even">Search Flow Cytometry Products</div></div></div></h2><div class="group-search-wrapper field-group-div"><div class="field field-name-field-pre-faceted-search field-type-et-faceted-search field-label-hidden"><div class="field-items"><div class="field-item even"><div class=""> <form action="/search" class="form-inline" method="get" id="faceted_search_bar"> <div class="form-group keyword-input"> <input type="hidden" name="rnd_applications" value="Flow Cytometry"> <input id="pc_facet" type="textfield" name="keywords" placeholder="Keywords"/> </div> <input type="submit" name="submit" value="Search" id="pc_submit" class="btn btn-primary"/> </form> </div> </div></div></div></div></div><div id="product-category-wrapper" class="row group-product-category-wrapper field-group-div"><div class="col-xs-12 col-sm-8 col-md-9 col-lg-9 col-xl-9 group-left-wrapper field-group-div"><div class="group-intro field-group-div"> <div class="paragraphs-items paragraphs-items-field-introduction paragraphs-items-field-introduction-full paragraphs-items-full"> <div class="field field-name-field-introduction field-type-paragraphs field-label-hidden"><div class="field-items"><div class="field-item even"><div class="entity entity-paragraphs-item paragraphs-item-title-and-basic-html" about="" typeof=""> <div class="content"> <div class="group-basic-div field-group-div"><h2 class="group-title-wrapper field-group-html-element"><div class="field field-name-field-title field-type-text field-label-hidden"><div class="field-items"><div class="field-item even">Flow Cytometry Workflow Solutions </div></div></div></h2><p class="group-desc field-group-html-element"><div class="field field-name-field-teaser-body field-type-text-with-summary field-label-hidden"><div class="field-items"><div class="field-item even"><div id="flow-cytometry-portfolio"> <p>Flow Cytometry is a technique used for identifying cell populations as well as measuring cell surface and intracellular molecules. Cells are suspended in a buffer and flow single file through an instrument called a flow cytometer, allowing multiple parameters to be collected and measured at a high speed rate. This creates a significant advantage over other antibody techniques due to a large number of cells being analyzed in such short amount of time. Flow Cytometry is used in research for applications such as immunophenotyping, DNA studies, cell cycle analysis, and fluorescence-activated cell sorting (FACS).</p> <p>R&D Systems offers a wide range of flow cytometry antibodies and products to fit your cell selection and detection workflow. We have workflow solutions, whether you are:</p> <ul> <li>Starting with a mixed cell population and wish to enrich for a specific subset of cells</li> <li>Differentiating and expanding immune cells or stem cells</li> <li>Assembling a flow cytometry antibody panel to detect and define cell populations</li> </ul> <p>We provide a wide range of over 15+ enzymatic and fluorescent conjugate options specifically designed to monitor protein expression. <a href="/products/conjugated-primary-antibodies">View our complete list of conjugated antibodies.</a></p>   <p><strong>Contents</strong></p> <div class="row"> <div class="col-md-6"> <ul style="list-style:none;"> <li style="padding:5px 0"><a href="#cell-selection">Cell Selection</a></li> <li style="padding:5px 0"><a href="#cell-differentiation">Cell Differentiation</a></li> <li style="padding:5px 0"><a href="#cell-detection">Cell Detection</a></li> </ul> </div> <div class="col-md-6"> <ul style="list-style:none;"> <li style="padding:5px 0"><a href="#flow-protocols">Flow Cytometry Protocols</a></li> <li style="padding:5px 0"><a href="#flow-resources">Additional Flow Cytometry Resources</a></li> </ul> </div> </div> </div> <p><a id="cell-selection" name="cell-selection"></a></p> </div></div></div></p></div> </div> </div> </div></div></div></div> </div><div class="group-basic-video field-group-div"> <div class="paragraphs-items paragraphs-items-field-basic-paragraph paragraphs-items-field-basic-paragraph-full paragraphs-items-full"> <div class="field field-name-field-basic-paragraph field-type-paragraphs field-label-hidden"><div class="field-items"><div class="field-item even"><div class="entity entity-paragraphs-item paragraphs-item-title-and-basic-html" about="" typeof=""> <div class="content"> <div class="group-basic-div field-group-div"><h2 class="group-title-wrapper field-group-html-element"><div class="field field-name-field-title field-type-text field-label-hidden"><div class="field-items"><div class="field-item even">Cell Selection </div></div></div></h2><p class="group-desc field-group-html-element"><div class="field field-name-field-teaser-body field-type-text-with-summary field-label-hidden"><div class="field-items"><div class="field-item even"><div> <p>Utilize our products to select and enrich for specific cell populations. We offer both negative and positive cell selection products.</p> <blockquote> <p><a href="/products/magcellect-tm-cell-selection-kits-reagents"><strong>MagCellect Cell Selection Kits</strong></a><br /> Magnetic bead-based selection of cell populations offered in both positive and negative selection</p> <p><a href="/products/supplemental-cell-selection-reagents-magcellect-magnet"><strong>Supplemental Cell Selection Reagents & MagCellect Cell Selection Magnet</strong></a><br /> Additional reagents for cell selection and detection experiments</p> </blockquote> </div> <p><a id="cell-differentiation" name="cell-differentiation"></a></p> </div></div></div></p></div> </div> </div> </div><div class="field-item odd"><div class="entity entity-paragraphs-item paragraphs-item-title-and-basic-html" about="" typeof=""> <div class="content"> <div class="group-basic-div field-group-div"><h2 class="group-title-wrapper field-group-html-element"><div class="field field-name-field-title field-type-text field-label-hidden"><div class="field-items"><div class="field-item even">Cell Differentiation </div></div></div></h2><p class="group-desc field-group-html-element"><div class="field field-name-field-teaser-body field-type-text-with-summary field-label-hidden"><div class="field-items"><div class="field-item even"><div> <p>Take advantage of our optimized cytokine combinations and media selection to differentiate your cells.</p> <blockquote> <p><a href="/products/cell-x-vivo-differentiation-expansion-kits"><strong>CellXVivo<sup>™</sup> Cytokine Cocktails for Differentiation</strong></a><br /> Optimized reagents for differentiation or expansion of leukocyte populations in culture</p> <p><a href="/products/stem-cell-differentiation-kits-and-media-supplements"><strong>Stem Cell Differentiation Kits and Media Supplements</strong></a><br /> Directed differentiation of pluripotent stem cells into intermediate and terminally differentiated cell types</p> <p><a href="/products/cell-culture"><strong>Cell Culture Products</strong></a><br /> High-quality and affordable reagents for optimal performance</p> <p><a href="/products/cell-culture-media-supplements"><strong>Cell Culture Media Supplements</strong></a><br /> Supplements available for laboratory cell lines as well as specialized cells, such as primary neurons and stem cells</p> </blockquote> </div> <p><a id="cell-detection" name="cell-detection"></a></p> </div></div></div></p></div> </div> </div> </div><div class="field-item even"><div class="entity entity-paragraphs-item paragraphs-item-title-and-basic-html" about="" typeof=""> <div class="content"> <div class="group-basic-div field-group-div"><h2 class="group-title-wrapper field-group-html-element"><div class="field field-name-field-title field-type-text field-label-hidden"><div class="field-items"><div class="field-item even">Cell Detection </div></div></div></h2><p class="group-desc field-group-html-element"><div class="field field-name-field-teaser-body field-type-text-with-summary field-label-hidden"><div class="field-items"><div class="field-item even"><div> <p>Visualize your cell with our wide range of cell detection tools.</p> <blockquote> <p><a href="/search?keywords=primary%20antibodies&category=Primary%20Antibodies&rnd_applications=Flow%20Cytometry"><strong>Flow Cytometry Antibodies</strong></a><br /> A wide range of unconjugated and conjugated validated primary antibodies for flow cytometry</p> <p><a href="/search?keywords=HLDA"><strong>HLDA Antibodies</strong></a><br /> Clones used by the HLDA to establish CD designation. The majority developed at R&D Systems</p> <p><a href="/products/secondary-antibodies"><strong>Secondary Antibodies and Antibody Isotype Controls</strong></a><br /> Find the right flow cytometry <a href="/search?category=Secondary Antibodies&rnd_applications=Flow Cytometry">secondary antibody</a> and <a href="/search?category=Isotype Controls">isotype control </a>that correlate to your primary antibody</p> <p><a href="/products/multi-color-flow-cytometry-kits"><strong>Multi-Color Flow Cytometry Kits</strong></a><br /> Simultaneously label cells with multiple fluorescent label antibodies</p> <p><a href="/products/flow-cytometry-supplemental-reagents"><strong>Flow Cytometry Supplemental Reagents</strong></a><br /> Staining, fixation, and permeabilization Flow Cytometry buffers</p> <p><a href="/product-highlights/antibodies-cytof"><strong>CyTOF-ready Antibodies</strong></a><br /> Antibodies ready to be conjugated for CyTOF Mass Cytometry analysis</p> <p><a href="https://www.proteinsimple.com/milo.html" target="_blank"><strong>Milo<sup>™</sup> by Protein Simple</strong></a><br /> A Single-Cell Western platform to measure thousands of cells in a single run</p> </blockquote> </div> <p><a id="flow-protocols" name="flow-protocols"></a></p> </div></div></div></p></div> </div> </div> </div><div class="field-item odd"><div class="entity entity-paragraphs-item paragraphs-item-title-and-basic-html" about="" typeof=""> <div class="content"> <div class="group-basic-div field-group-div"><h2 class="group-title-wrapper field-group-html-element"><div class="field field-name-field-title field-type-text field-label-hidden"><div class="field-items"><div class="field-item even">Flow Cytometry Protocols </div></div></div></h2><p class="group-desc field-group-html-element"><div class="field field-name-field-teaser-body field-type-text-with-summary field-label-hidden"><div class="field-items"><div class="field-item even"><div> <p>Protocols created and optimized to stain intracellular or membrane-associated molecules.</p> <blockquote> <p><a href="/resources/protocols/flow-cytometry-protocol-staining-membrane-associated-proteins-suspended-cells"><strong>Flow Cytometry Staining Protocol for Membrane-associated Proteins</strong></a><br /> Stain proteins located on the cell surface</p> <p><a href="/resources/protocols/flow-cytometry-protocol-staining-intracellular-molecules-using-alcohol"><strong>Intracellular Staining Flow Cytometry Protocol using Alcohol</strong></a><br /> Analyze intracellular targets by permeabilizing cells with alcohol</p> <p><a href="/resources/protocols/flow-cytometry-protocol-staining-intracellular-molecules-using-detergents"><strong>Intracellular Flow Cytometry Protocol using Detergents</strong></a><br /> View intracellular molecules using detergents for fixation and permeabilization</p> <p><a href="/resources/protocols/flow-cytometry-protocol-analysis-cell-viability-using-propidium-iodide"><strong>Propidium Iodide Flow Cytometry Protocol</strong></a><br /> Determine cell viability with the use of propidium iodide</p> </blockquote> </div> <p><a id="flow-resources" name="flow-resources"></a></p> </div></div></div></p></div> </div> </div> </div><div class="field-item even"><div class="entity entity-paragraphs-item paragraphs-item-title-and-basic-html" about="" typeof=""> <div class="content"> <div class="group-basic-div field-group-div"><h2 class="group-title-wrapper field-group-html-element"><div class="field field-name-field-title field-type-text field-label-hidden"><div class="field-items"><div class="field-item even">Additional Flow Cytometry Resources</div></div></div></h2><p class="group-desc field-group-html-element"><div class="field field-name-field-teaser-body field-type-text-with-summary field-label-hidden"><div class="field-items"><div class="field-item even"><div> <p><strong>Blogs and Webinars</strong></p> <blockquote> <p><a href="/blog/ror-gamma-t-and-th17-cells-new-highly-specific-antibody-will-find-what-you-have-been-missing">ROR gamma t and Th17 Cells: New Highly-specific Antibody Will Find What You Have Been Missing </a></p> <p><a href="/blog/interactive-cell-marker-tool-cd-markers-and-beyond">Interactive Cell Marker Tool: CD Markers and Beyond </a></p> <p><a href="/blog/relax-and-go-flow-demystifying-multi-parameter-flow-cytometry">Relax and Go with the Flow: Demystifying Multi-Parameter Flow Cytometry </a></p> <p><a href="/blog/webinar-2-turning-flow-cytometry-upside-down-and-inside-out-cellular-function-revealed">Turning Flow Cytometry Upside Down and Inside Out: Cellular Function Revealed </a></p> <p><a href="/resources/webinars/lessons-from-20-years-in-antibody-validation-and-reproducibility">Lessons from 20 Years in Antibody Validation and Reproducibility</a></p> </blockquote> <p><strong>Literature</strong></p> <blockquote> <p><a href="/resources/white-paper/development-validated-monoclonal-antibodies">Development of Validated Monoclonal Antibodies </a></p> <p><a href="/resources/posters/phenotypic-characterization-human-m1-and-m2a-macrophages-cultured-different-serums">Phenotypic Characterization of Human M1 and M2a Macrophages Cultured with Different Serums </a></p> <p><a href="/resources/posters/identification-novel-human-and-mouse-regulatory-t-cell-surface-markers-flow-cytometry">Identification of Novel Human and Mouse Regulatory T cell Surface Markers via Flow Cytometry Screening </a></p> </blockquote> <p><strong>Product Brochures</strong></p> <blockquote> <p><a href="https://info.bio-techne.com/flow-cytometry-handbook?utm_source=rndsystems.com&utm_medium=banner&utm_campaign=Flow Cytometry Handbook&utm_term=brochures&utm_content=banner">Flow Cytometry eHandbook </a></p> <p><a href="https://resources.rndsystems.com/images/site/rnd-systems-antibody-services-br.pdf">Custom Antibody Services </a></p> <p><a href="https://resources.rndsystems.com/pdfs/brochures/br_immunology-workflow_mkt20-11103.pdf">Products for Immunology Research </a></p> <p><a href="https://resources.rndsystems.com/images/site/rnd-systems-nkcell-br.pdf">Products for Natural Killer Cell Research </a></p> <p><a href="https://resources.rndsystems.com/images/site/rnd-systems-ilcs-br.pdf">Tools for Innate Lymphoid Cell Research </a></p> <p><a href="https://resources.rndsystems.com/images/site/rnd-systems-bcells-br.pdf">B Cells </a></p> <p><a href="/resources/literature/methods-isolation-differentiation-and-characterization-cd4-t-cell-subsets">CD4<sup>+</sup> T Cell Subsets </a></p> </blockquote> </div> </div></div></div></p></div> </div> </div> </div></div></div></div> </div></div><div class="col-xs-12 col-sm-4 col-md-3 col-lg-3 col-xl-3 group-featured-content-wrapper field-group-div"><h2 class="field-group-html-element"><div class="field field-name-field-featured-content-title field-type-text field-label-hidden"><div 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