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biosensor</title> <meta name="description" content="Search results for: biosensor"> <meta name="keywords" content="biosensor"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none navbar-toggler ml-auto" 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id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="biosensor"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 140</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: biosensor</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">140</span> Effect of III-V Nitrides on Performance of Graphene-Gold SPR Biosensor</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Bijaya%20Kumar%20Sahoo">Bijaya Kumar Sahoo</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The effect of III-V nitride semiconductors on performance of a graphene-on-gold surface plasmon resonance (SPR) biosensor has been investigated theoretically. III-V nitrides (AlN, GaN and InN) have been grown between gold (Au) and graphene layers. The sensitivity and performance of the biosensor have been computed for with and without semiconductors. Due to superior electronic and optical properties, III-V nitrides demonstrate high sensitivity and performance over Si and Ge. The enhancement of evanescent electric field due to III-V nitrides have been computed and found highest for InN. The analysis shows that for a high-sensitive imaging biosensor the required optimal thickness of gold, InN and graphene are respectively 49 nm, 11 nm and 0.34 nm for the light of wavelength =633 nm (red He-Ne laser). This study suggests that InN would be a better choice for fabrication of new imaging SPR biosensors. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=SPR%20biosensor" title="SPR biosensor">SPR biosensor</a>, <a href="https://publications.waset.org/abstracts/search?q=optical%20properties" title=" optical properties"> optical properties</a>, <a href="https://publications.waset.org/abstracts/search?q=III-V%20nitrides" title=" III-V nitrides"> III-V nitrides</a>, <a href="https://publications.waset.org/abstracts/search?q=sensitivity" title=" sensitivity"> sensitivity</a>, <a href="https://publications.waset.org/abstracts/search?q=enhancement%20of%20electric%20field" title=" enhancement of electric field"> enhancement of electric field</a>, <a href="https://publications.waset.org/abstracts/search?q=performance%20of%20graphene%20gold%20SPR%20biosensor" title=" performance of graphene gold SPR biosensor"> performance of graphene gold SPR biosensor</a> </p> <a href="https://publications.waset.org/abstracts/46349/effect-of-iii-v-nitrides-on-performance-of-graphene-gold-spr-biosensor" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/46349.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">550</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">139</span> DNA-Based Gold Nanoprobe Biosensor to Detect Pork Contaminant</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Rizka%20Ardhiyana">Rizka Ardhiyana</a>, <a href="https://publications.waset.org/abstracts/search?q=Liesbetini%20Haditjaroko"> Liesbetini Haditjaroko</a>, <a href="https://publications.waset.org/abstracts/search?q=Sri%20Mulijani"> Sri Mulijani</a>, <a href="https://publications.waset.org/abstracts/search?q=Reki%20Ashadi%20Wicaksono"> Reki Ashadi Wicaksono</a>, <a href="https://publications.waset.org/abstracts/search?q=Raafqi%20Ranasasmita"> Raafqi Ranasasmita</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Designing a sensitive, specific and easy to use method to detect pork contamination in the food industry remains a major challenge. In the current study, we developed a sensitive thiol-bond AuNP-Probe biosensor that will change color when detecting pork DNA in the Cytochrome B region. The interaction between the biosensors and DNA sample is measured by spectrophotometer at 540 nm. The biosensor is made by reducing gold with trisodium citrate to produce gold nanoparticle with 39.05 nm diameter. The AuNP-Probe biosensor (gold nanoprobe) achieved 16.04 ng DNA/µl limit of detection and 53.48 ng DNA/µl limit of quantification. The linearity (R2) between color absorbance changes and DNA concentration is 0.9916. The biosensor has a good specificty as it does not cross-react with DNA of chicken and beef. To verify specificity towards the target sequence, PCR was tested to the target sequence and reacted to the PCR product with the biosensor. The PCR DNA isolate resulted in a 2.7 fold higher absorbance compared to pork-DNA isolate alone (without PCR). The sensitivity and specificity of the method show the promising application of the thiol-bond AuNP biosensor in pork-detection. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=biosensor" title="biosensor">biosensor</a>, <a href="https://publications.waset.org/abstracts/search?q=DNA%20probe" title=" DNA probe"> DNA probe</a>, <a href="https://publications.waset.org/abstracts/search?q=gold%20nanoparticle%20%28AuNP%29" title=" gold nanoparticle (AuNP)"> gold nanoparticle (AuNP)</a>, <a href="https://publications.waset.org/abstracts/search?q=pork%20meat" title=" pork meat"> pork meat</a>, <a href="https://publications.waset.org/abstracts/search?q=qPCR" title=" qPCR"> qPCR</a> </p> <a href="https://publications.waset.org/abstracts/72688/dna-based-gold-nanoprobe-biosensor-to-detect-pork-contaminant" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/72688.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">359</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">138</span> Eresa, Hospital General Universitario de Elche</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ashish%20Kumar%20Singh">Ashish Kumar Singh</a>, <a href="https://publications.waset.org/abstracts/search?q=Mehak%20Gulati"> Mehak Gulati</a>, <a href="https://publications.waset.org/abstracts/search?q=Neelam%20Verma"> Neelam Verma</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Arginine majorly acts as a substrate for the enzyme nitric oxide synthase (NOS) for the production of nitric oxide, a strong vasodilator. Current study demonstrated a novel amperometric approach for estimation of arginine using nitric oxide synthase. The enzyme was co-immobilized in carbon paste electrode with NADP+, FAD and BH4 as cofactors. The detection principle of the biosensor is enzyme NOS catalyzes the conversion of arginine into nitric oxide. The developed biosensor could able to detect up to 10-9M of arginine. The oxidation peak of NO was observed at 0.65V. The developed arginine biosensor was used to monitor arginine content in fruit juices. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=arginine" title="arginine">arginine</a>, <a href="https://publications.waset.org/abstracts/search?q=biosensor" title=" biosensor"> biosensor</a>, <a href="https://publications.waset.org/abstracts/search?q=carbon%20paste%20elctrode" title=" carbon paste elctrode"> carbon paste elctrode</a>, <a href="https://publications.waset.org/abstracts/search?q=nitric%20oxide" title=" nitric oxide"> nitric oxide</a> </p> <a href="https://publications.waset.org/abstracts/28880/eresa-hospital-general-universitario-de-elche" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/28880.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">424</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">137</span> An Electrochemical DNA Biosensor Based on Oracet Blue as a Label for Detection of Helicobacter pylori </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Saeedeh%20Hajihosseini">Saeedeh Hajihosseini</a>, <a href="https://publications.waset.org/abstracts/search?q=Zahra%20Aghili"> Zahra Aghili</a>, <a href="https://publications.waset.org/abstracts/search?q=Navid%20Nasirizadeh"> Navid Nasirizadeh</a> </p> <p class="card-text"><strong>Abstract:</strong></p> An innovative method of a DNA electrochemical biosensor based on Oracet Blue (OB) as an electroactive label and gold electrode (AuE) for detection of Helicobacter pylori, was offered. A single–stranded DNA probe with a thiol modification was covalently immobilized on the surface of the AuE by forming an Au–S bond. Differential pulse voltammetry (DPV) was used to monitor DNA hybridization by measuring the electrochemical signals of reduction of the OB binding to double– stranded DNA (ds–DNA). Our results showed that OB–based DNA biosensor has a decent potential for detection of single–base mismatch in target DNA. Selectivity of the proposed DNA biosensor was further confirmed in the presence of non–complementary and complementary DNA strands. Under optimum conditions, the electrochemical signal had a linear relationship with the concentration of the target DNA ranging from 0.3 nmol L-1 to 240.0 nmol L-1, and the detection limit was 0.17 nmol L-1, whit a promising reproducibility and repeatability. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=DNA%20biosensor" title="DNA biosensor">DNA biosensor</a>, <a href="https://publications.waset.org/abstracts/search?q=oracet%20blue" title=" oracet blue"> oracet blue</a>, <a href="https://publications.waset.org/abstracts/search?q=Helicobacter%20pylori" title=" Helicobacter pylori"> Helicobacter pylori</a>, <a href="https://publications.waset.org/abstracts/search?q=electrode%20%28AuE%29" title=" electrode (AuE)"> electrode (AuE)</a> </p> <a href="https://publications.waset.org/abstracts/53867/an-electrochemical-dna-biosensor-based-on-oracet-blue-as-a-label-for-detection-of-helicobacter-pylori" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/53867.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">266</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">136</span> A Contactless Capacitive Biosensor for Muscle Activity Measurement</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Charn%20Loong%20Ng">Charn Loong Ng</a>, <a href="https://publications.waset.org/abstracts/search?q=Mamun%20Bin%20Ibne%20Reaz"> Mamun Bin Ibne Reaz</a> </p> <p class="card-text"><strong>Abstract:</strong></p> As elderly population grows globally, the percentage of people diagnosed with musculoskeletal disorder (MSD) increase proportionally. Electromyography (EMG) is an important biosignal that contributes to MSD’s clinical diagnose and recovery process. Conventional conductive electrode has many disadvantages in the continuous EMG measurement application. This research has design a new surface EMG biosensor based on the parallel-plate capacitive coupling principle. The biosensor is developed by using a double-sided PCB with having one side of the PCB use to construct high input impedance circuitry while the other side of the copper (CU) plate function as biosignal sensing metal plate. The metal plate is insulated using kapton tape for contactless application. The result implicates that capacitive biosensor is capable to constantly capture EMG signal without having galvanic contact to human skin surface. However, there are noticeable noise couple into the measured signal. Post signal processing is needed in order to present a clean and significant EMG signal. A complete design of single ended, non-contact, high input impedance, front end EMG biosensor is presented in this paper. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=contactless" title="contactless">contactless</a>, <a href="https://publications.waset.org/abstracts/search?q=capacitive" title=" capacitive"> capacitive</a>, <a href="https://publications.waset.org/abstracts/search?q=biosensor" title=" biosensor"> biosensor</a>, <a href="https://publications.waset.org/abstracts/search?q=electromyography" title=" electromyography"> electromyography</a> </p> <a href="https://publications.waset.org/abstracts/29854/a-contactless-capacitive-biosensor-for-muscle-activity-measurement" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/29854.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">450</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">135</span> Sensitivity Enhancement in Graphene Based Surface Plasmon Resonance (SPR) Biosensor</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Angad%20S.%20Kushwaha">Angad S. Kushwaha</a>, <a href="https://publications.waset.org/abstracts/search?q=Rajeev%20Kumar"> Rajeev Kumar</a>, <a href="https://publications.waset.org/abstracts/search?q=Monika%20Srivastava"> Monika Srivastava</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20K.%20Srivastava"> S. K. Srivastava</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A lot of research work is going on in the field of graphene based SPR biosensor. In the conventional SPR based biosensor, graphene is used as a biomolecular recognition element. Graphene adsorbs biomolecules due to carbon based ring structure through sp2 hybridization. The proposed SPR based biosensor configuration will open a new avenue for efficient biosensing by taking the advantage of Graphene and its fascinating nanofabrication properties. In the present study, we have studied an SPR biosensor based on graphene mediated by Zinc Oxide (ZnO) and Gold. In the proposed structure, prism (BK7) base is coated with Zinc Oxide followed by Gold and Graphene. Using the waveguide approach by transfer matrix method, the proposed structure has been investigated theoretically. We have analyzed the reflectance versus incidence angle curve using He-Ne laser of wavelength 632.8 nm. Angle, at which the reflectance is minimized, termed as SPR angle. The shift in SPR angle is responsible for biosensing. From the analysis of reflectivity curve, we have found that there is a shift in SPR angle as the biomolecules get attached on the graphene surface. This graphene layer also enhances the sensitivity of the SPR sensor as compare to the conventional sensor. The sensitivity also increases by increasing the no of graphene layer. So in our proposed biosensor we have found minimum possible reflectivity with optimum level of sensitivity. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=biosensor" title="biosensor">biosensor</a>, <a href="https://publications.waset.org/abstracts/search?q=sensitivity" title=" sensitivity"> sensitivity</a>, <a href="https://publications.waset.org/abstracts/search?q=surface%20plasmon%20resonance" title=" surface plasmon resonance"> surface plasmon resonance</a>, <a href="https://publications.waset.org/abstracts/search?q=transfer%20matrix%20method" title=" transfer matrix method"> transfer matrix method</a> </p> <a href="https://publications.waset.org/abstracts/40534/sensitivity-enhancement-in-graphene-based-surface-plasmon-resonance-spr-biosensor" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/40534.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">417</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">134</span> Simulation Model of Biosensor Based on Gold Nanoparticles</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Kholod%20Hajo">Kholod Hajo</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In this study COMSOL Multiphysics was used to design lateral flow biosensors (LFBs) which provide advantages in low cost, simplicity, rapidity, stability and portability thus making LFBs popular in biomedical, agriculture, food and environmental sciences. This study was focused on simulation model of biosensor based on gold nanoparticles (GNPs) designed using software package (COMSOL Multiphysics), the magnitude of the laminar velocity field in the flow cell, concentration distribution in the analyte stream and surface coverage of adsorbed species and average fractional surface coverage of adsorbed analyte were discussed from the model and couples of suggestion was given in order to functionalize GNPs and to increase the accuracy of the biosensor design, all above were obtained acceptable results. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=model" title="model">model</a>, <a href="https://publications.waset.org/abstracts/search?q=gold%20nanoparticles" title=" gold nanoparticles"> gold nanoparticles</a>, <a href="https://publications.waset.org/abstracts/search?q=biosensor" title=" biosensor"> biosensor</a>, <a href="https://publications.waset.org/abstracts/search?q=COMSOL%20Multiphysics" title=" COMSOL Multiphysics"> COMSOL Multiphysics</a> </p> <a href="https://publications.waset.org/abstracts/65339/simulation-model-of-biosensor-based-on-gold-nanoparticles" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/65339.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">257</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">133</span> Construction and Performance of Nanocomposite-Based Electrochemical Biosensor</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jianfang%20Wang">Jianfang Wang</a>, <a href="https://publications.waset.org/abstracts/search?q=Xianzhe%20Chen"> Xianzhe Chen</a>, <a href="https://publications.waset.org/abstracts/search?q=Zhuoliang%20Liu"> Zhuoliang Liu</a>, <a href="https://publications.waset.org/abstracts/search?q=Cheng-An%20Tao"> Cheng-An Tao</a>, <a href="https://publications.waset.org/abstracts/search?q=Yujiao%20Li"> Yujiao Li</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Organophosphorus (OPs) pesticide used as insecticides are widely used in agricultural pest control, household and storage deworming. The detection of pesticides needs more simple and efficient methods. One of the best ways is to make electrochemical biosensors. In this paper, an electrochemical enzyme biosensor based on acetylcholine esterase (AChE) was constructed, and its sensing properties and sensing mechanisms were studied. Reduced graphene oxide-polydopamine complexes (RGO-PDA), gold nanoparticles (AuNPs) and silver nanoparticles (AgNPs) were prepared firstly and composited with AChE and chitosan (CS), then fixed on the glassy carbon electrode (GCE) surface to construct the biosensor GCE/RGO-PDA-AuNPs-AgNPs-AChE-CS by one-pot method. The results show that graphene oxide (GO) can be reduced by dopamine (DA) and dispersed well in RGO-PDA complexes. And the composites have a synergistic catalysis effect and can improve the surface resistance of GCE. The biosensor selectively can detect acetylcholine (ACh) and OPs pesticide with good linear range and high sensitivity. The performance of the biosensor is affected by the ratio and adding ways of AChE and the adding of AuNPs and AChE. And the biosensor can achieve a detection limit of 2.4 ng/L for methyl parathion and a wide linear detection range of 0.02 ng/L ~ 80 ng/L, and has excellent stability, good anti-interference ability, and excellent preservation performance, indicating that the sensor has practical value. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=acetylcholine%20esterase" title="acetylcholine esterase">acetylcholine esterase</a>, <a href="https://publications.waset.org/abstracts/search?q=electrochemical%20biosensor" title=" electrochemical biosensor"> electrochemical biosensor</a>, <a href="https://publications.waset.org/abstracts/search?q=nanoparticles" title=" nanoparticles"> nanoparticles</a>, <a href="https://publications.waset.org/abstracts/search?q=organophosphates" title=" organophosphates"> organophosphates</a>, <a href="https://publications.waset.org/abstracts/search?q=reduced%20graphene%20oxide" title=" reduced graphene oxide"> reduced graphene oxide</a> </p> <a href="https://publications.waset.org/abstracts/116820/construction-and-performance-of-nanocomposite-based-electrochemical-biosensor" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/116820.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">112</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">132</span> Improved Reuse and Storage Performances at Room Temperature of a New Environmental-Friendly Lactate Oxidase Biosensor Made by Ambient Electrospray Deposition</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Antonella%20Cartoni">Antonella Cartoni</a>, <a href="https://publications.waset.org/abstracts/search?q=Mattea%20Carmen%20Castrovilli"> Mattea Carmen Castrovilli</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A biosensor for lactate detection has been developed using an environmentally friendly approach. The biosensor is based on lactate oxidase (LOX) and has remarkable capabilities for reuse and storage at room temperature. The manufacturing technique employed is ambient electrospray deposition (ESD), which enables efficient and sustainable immobilization of the LOX enzyme on a cost-effective com-mercial screen-printed Prussian blue/carbon electrode (PB/C-SPE). The study demonstrates that the ESD technology allows the biosensor to be stored at ambient pressure and temperature for extended periods without affecting the enzymatic activity. The biosensor can be stored for up to 90 days without requiring specific storage conditions, and it can be reused for up to 24 measurements on both freshly prepared electrodes and electrodes that are three months old. The LOX-based biosensor exhibits a lin-ear range of lactate detection between 0.1 and 1 mM, with a limit of detection of 0.07±0.02 mM. Ad-ditionally, it does not exhibit any memory effects. The immobilization process does not involve the use of entrapment matrices or hazardous chemicals, making it environmentally sustainable and non-toxic compared to current methods. Furthermore, the application of a electrospray deposition cycle on previously used biosensors rejuvenates their performance, making them comparable to freshly made biosensors. This highlights the excellent recycling potential of the technique, eliminating the waste as-sociated with disposable devices. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=green%20friendly" title="green friendly">green friendly</a>, <a href="https://publications.waset.org/abstracts/search?q=reuse" title=" reuse"> reuse</a>, <a href="https://publications.waset.org/abstracts/search?q=storage%20performance" title=" storage performance"> storage performance</a>, <a href="https://publications.waset.org/abstracts/search?q=immobilization" title=" immobilization"> immobilization</a>, <a href="https://publications.waset.org/abstracts/search?q=matrix-free" title=" matrix-free"> matrix-free</a>, <a href="https://publications.waset.org/abstracts/search?q=electrospray%20deposition" title=" electrospray deposition"> electrospray deposition</a>, <a href="https://publications.waset.org/abstracts/search?q=biosensor" title=" biosensor"> biosensor</a>, <a href="https://publications.waset.org/abstracts/search?q=lactate%20oxidase" title=" lactate oxidase"> lactate oxidase</a>, <a href="https://publications.waset.org/abstracts/search?q=enzyme" title=" enzyme"> enzyme</a> </p> <a href="https://publications.waset.org/abstracts/175645/improved-reuse-and-storage-performances-at-room-temperature-of-a-new-environmental-friendly-lactate-oxidase-biosensor-made-by-ambient-electrospray-deposition" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/175645.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">65</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">131</span> Data Model to Predict Customize Skin Care Product Using Biosensor</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ashi%20Gautam">Ashi Gautam</a>, <a href="https://publications.waset.org/abstracts/search?q=Isha%20Shukla"> Isha Shukla</a>, <a href="https://publications.waset.org/abstracts/search?q=Akhil%20Seghal"> Akhil Seghal</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Biosensors are analytical devices that use a biological sensing element to detect and measure a specific chemical substance or biomolecule in a sample. These devices are widely used in various fields, including medical diagnostics, environmental monitoring, and food analysis, due to their high specificity, sensitivity, and selectivity. In this research paper, a machine learning model is proposed for predicting the suitability of skin care products based on biosensor readings. The proposed model takes in features extracted from biosensor readings, such as biomarker concentration, skin hydration level, inflammation presence, sensitivity, and free radicals, and outputs the most appropriate skin care product for an individual. This model is trained on a dataset of biosensor readings and corresponding skin care product information. The model's performance is evaluated using several metrics, including accuracy, precision, recall, and F1 score. The aim of this research is to develop a personalised skin care product recommendation system using biosensor data. By leveraging the power of machine learning, the proposed model can accurately predict the most suitable skin care product for an individual based on their biosensor readings. This is particularly useful in the skin care industry, where personalised recommendations can lead to better outcomes for consumers. The developed model is based on supervised learning, which means that it is trained on a labeled dataset of biosensor readings and corresponding skin care product information. The model uses these labeled data to learn patterns and relationships between the biosensor readings and skin care products. Once trained, the model can predict the most suitable skin care product for an individual based on their biosensor readings. The results of this study show that the proposed machine learning model can accurately predict the most appropriate skin care product for an individual based on their biosensor readings. The evaluation metrics used in this study demonstrate the effectiveness of the model in predicting skin care products. This model has significant potential for practical use in the skin care industry for personalised skin care product recommendations. The proposed machine learning model for predicting the suitability of skin care products based on biosensor readings is a promising development in the skin care industry. The model's ability to accurately predict the most appropriate skin care product for an individual based on their biosensor readings can lead to better outcomes for consumers. Further research can be done to improve the model's accuracy and effectiveness. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=biosensors" title="biosensors">biosensors</a>, <a href="https://publications.waset.org/abstracts/search?q=data%20model" title=" data model"> data model</a>, <a href="https://publications.waset.org/abstracts/search?q=machine%20learning" title=" machine learning"> machine learning</a>, <a href="https://publications.waset.org/abstracts/search?q=skin%20care" title=" skin care"> skin care</a> </p> <a href="https://publications.waset.org/abstracts/164611/data-model-to-predict-customize-skin-care-product-using-biosensor" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/164611.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">97</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">130</span> Amperometric Biosensor for Glucose Determination Based on a Recombinant Mn Peroxidase from Corn Cross-linked to a Gold Electrode</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Anahita%20Izadyar">Anahita Izadyar</a>, <a href="https://publications.waset.org/abstracts/search?q=My%20Ni%20Van"> My Ni Van</a>, <a href="https://publications.waset.org/abstracts/search?q=Kayleigh%20Amber%20Rodriguez"> Kayleigh Amber Rodriguez</a>, <a href="https://publications.waset.org/abstracts/search?q=Ilwoo%20Seok"> Ilwoo Seok</a>, <a href="https://publications.waset.org/abstracts/search?q=Elizabeth%20E.%20Hood"> Elizabeth E. Hood</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Using a recombinant enzyme derived from corn and a simple modification, we fabricated a facile, fast, and cost-beneficial biosensor to measure glucose. The Nafion/ Plant Produced Mn Peroxidase (PPMP)– glucose oxidase (GOx)- Bovine serum albumin (BSA) /Au electrode showed an excellent amperometric response to detect glucose. This biosensor is capable of responding to a wide range of glucose—20.0 µM−15.0 mM and has a lower detection limit (LOD) of 2.90µM. The reproducibility response using six electrodes is also very substantial and indicates the high capability of this biosensor to detect a wide range of 3.10±0.19µM to 13.2±1.8 mM glucose concentration. Selectivity of this electrode was investigated in an optimized experimental solution contains 10% diet green tea with citrus containing ascorbic acid (AA), and citric acid (CA) in a wide concentration of glucose at 0.02 to 14.0mM with an LOD of 3.10µM. Reproducibility was also investigated using 4 electrodes in this sample and shows notable results in the wide concentration range of 3.35±0.45µM to of 13.0 ± 0.81 mM. We also used other voltammetry methods to evaluate this biosensor. We applied linear sweep voltammetry (LSV) and this technique shows a wide range of 0.10−15.0 mM to detect glucose with a lower detection limit of 19.5µM. The performance and strength of this enzyme biosensor were the simplicity, wide linear ranges, sensitivities, selectivity, and low limits of detection. We expect that the modified biosensor has the potential for monitoring various biofluids. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=plant-produced%20manganese%20peroxidase" title="plant-produced manganese peroxidase">plant-produced manganese peroxidase</a>, <a href="https://publications.waset.org/abstracts/search?q=enzyme-based%20biosensors" title=" enzyme-based biosensors"> enzyme-based biosensors</a>, <a href="https://publications.waset.org/abstracts/search?q=glucose" title=" glucose"> glucose</a>, <a href="https://publications.waset.org/abstracts/search?q=modified%20gold%20electrode" title=" modified gold electrode"> modified gold electrode</a>, <a href="https://publications.waset.org/abstracts/search?q=glucose%20oxidase" title=" glucose oxidase"> glucose oxidase</a> </p> <a href="https://publications.waset.org/abstracts/133907/amperometric-biosensor-for-glucose-determination-based-on-a-recombinant-mn-peroxidase-from-corn-cross-linked-to-a-gold-electrode" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/133907.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">139</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">129</span> Fiber Optic Asparagine Biosensor for Fruit Juices by Co-Immobilization of L-Asparaginase and Phenol Red</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mandeep%20Kataria">Mandeep Kataria</a>, <a href="https://publications.waset.org/abstracts/search?q=Ritu%20Narula"> Ritu Narula</a>, <a href="https://publications.waset.org/abstracts/search?q=Navneet%20Kaur"> Navneet Kaur</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Asparagine is vital amino acid which is required for the development of brain and it regulates the equilibrium of central nervous system. Asparagine is the chief amino acid that forms acrylamide in baked food by reacting with reducing sugars at high temperature ( Millard Reaction i.e. amino acids and sugars give new flavors at high temperature). It can also be a parameter of freshness in fruit juices because on storage of juices at 37°C caused an 87% loss in the total free amino acids and major decrease was recorded in asparagine contents. With this significance of monitoring asparagine, in the present work a biosensor for determining asparagine in fruit juices is developed. For the construction of biosensor L-asparaginase enzyme (0.5 IU) was co-immobilized with phenol red on TEOS chitosan sol-gel plastic disc and fixed on the fiber optic tip. Tip was immersed in a cell having 5ml of substrate and absorption was noted at response time of 5 min with 10-1 - 10-10 M concentrations of asparagine at 538 nm. L-asparaginase was extracted and from Solanum nigrum Asparagine biosensor was applied fruit juices on the monitoring asparagine contents. L-asparagine concentration found to be present in fruit juices like Guava Juice, Apple Juice, Mango Juice, Litchi juice, Strawberry juice, Pineapple juice Lemon juice, and Orange juice. Hence the developed biosensor has commercial aspects in quality insurance of fruit juices. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=fiber%20optic%20biosensor" title="fiber optic biosensor">fiber optic biosensor</a>, <a href="https://publications.waset.org/abstracts/search?q=chitosan" title=" chitosan"> chitosan</a>, <a href="https://publications.waset.org/abstracts/search?q=teos" title=" teos"> teos</a>, <a href="https://publications.waset.org/abstracts/search?q=l-asparaginase" title=" l-asparaginase"> l-asparaginase</a> </p> <a href="https://publications.waset.org/abstracts/47241/fiber-optic-asparagine-biosensor-for-fruit-juices-by-co-immobilization-of-l-asparaginase-and-phenol-red" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/47241.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">289</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">128</span> Urea Amperometric Biosensor Based on Entrapment Immobilization of Urease onto a Nanostructured Polypyrrol and Multi-Walled Carbon Nanotube</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Hamide%20Amani">Hamide Amani</a>, <a href="https://publications.waset.org/abstracts/search?q=Afshin%20FarahBakhsh"> Afshin FarahBakhsh</a>, <a href="https://publications.waset.org/abstracts/search?q=Iman%20Farahbakhsh"> Iman Farahbakhsh</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In this paper, an amprometric biosensor based on surface modified polypyrrole (PPy) has been developed for the quantitative estimation of urea in aqueous solutions. The incorporation of urease (Urs) into a bipolymeric substrate consisting of PPy was performed by entrapment to the polymeric matrix, PPy acts as amperometric transducer in these biosensors. To increase the membrane conductivity, multi-walled carbon nanotubes (MWCNT) were added to the PPy solution. The entrapped MWCNT in PPy film and the bipolymer layers were prepared for construction of Pt/PPy/MWCNT/Urs. Two different configurations of working electrodes were evaluated to investigate the potential use of the modified membranes in biosensors. The evaluation of two different configurations of working electrodes suggested that the second configuration, which was composed of an electrode-mediator-(pyrrole and multi-walled carbon nanotube) structure and enzyme, is the best candidate for biosensor applications. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=urea%20biosensor" title="urea biosensor">urea biosensor</a>, <a href="https://publications.waset.org/abstracts/search?q=polypyrrole" title=" polypyrrole"> polypyrrole</a>, <a href="https://publications.waset.org/abstracts/search?q=multi-walled%20carbon%20nanotube" title=" multi-walled carbon nanotube"> multi-walled carbon nanotube</a>, <a href="https://publications.waset.org/abstracts/search?q=urease" title=" urease"> urease</a> </p> <a href="https://publications.waset.org/abstracts/86192/urea-amperometric-biosensor-based-on-entrapment-immobilization-of-urease-onto-a-nanostructured-polypyrrol-and-multi-walled-carbon-nanotube" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/86192.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">329</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">127</span> A Sensitive Approach on Trace Analysis of Methylparaben in Wastewater and Cosmetic Products Using Molecularly Imprinted Polymer </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Soukaina%20Motia">Soukaina Motia</a>, <a href="https://publications.waset.org/abstracts/search?q=Nadia%20El%20Alami%20El%20Hassani"> Nadia El Alami El Hassani</a>, <a href="https://publications.waset.org/abstracts/search?q=Alassane%20Diouf"> Alassane Diouf</a>, <a href="https://publications.waset.org/abstracts/search?q=Benachir%20Bouchikhi"> Benachir Bouchikhi</a>, <a href="https://publications.waset.org/abstracts/search?q=Nezha%20El%20Bari"> Nezha El Bari </a> </p> <p class="card-text"><strong>Abstract:</strong></p> Parabens are the antimicrobial molecules largely used in cosmetic products as a preservative agent. Among them, the methylparaben (MP) is the most frequently used ingredient in cosmetic preparations. Nevertheless, their potential dangers led to the development of sensible and reliable methods for their determination in environmental samples. Firstly, a sensitive and selective molecular imprinted polymer (MIP) based on screen-printed gold electrode (Au-SPE), assembled on a polymeric layer of carboxylated poly(vinyl-chloride) (PVC-COOH), was developed. After the template removal, the obtained material was able to rebind MP and discriminate it among other interfering species such as glucose, sucrose, and citric acid. The behavior of molecular imprinted sensor was characterized by Cyclic Voltammetry (CV), Differential Pulse Voltammetry (DPV) and Electrochemical Impedance Spectroscopy (EIS) techniques. Then, the biosensor was found to have a linear detection range from 0.1 pg.mL<sup>-1</sup> to 1 ng.mL<sup>-1</sup> and a low limit of detection of 0.12 fg.mL<sup>-1</sup> and 5.18 pg.mL<sup>-1</sup> by DPV and EIS, respectively. For applications, this biosensor was employed to determine MP content in four wastewaters in Meknes city and two cosmetic products (shower gel and shampoo). The operational reproducibility and stability of this biosensor were also studied. Secondly, another MIP biosensor based on tungsten trioxide (WO<sub>3</sub>) functionalized by gold nanoparticles (Au-NPs) assembled on a polymeric layer of PVC-COOH was developed. The main goal was to increase the sensitivity of the biosensor. The developed MIP biosensor was successfully applied for the MP determination in wastewater samples and cosmetic products. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cosmetic%20products" title="cosmetic products">cosmetic products</a>, <a href="https://publications.waset.org/abstracts/search?q=methylparaben" title=" methylparaben"> methylparaben</a>, <a href="https://publications.waset.org/abstracts/search?q=molecularly%20imprinted%20polymer" title=" molecularly imprinted polymer"> molecularly imprinted polymer</a>, <a href="https://publications.waset.org/abstracts/search?q=wastewater" title=" wastewater"> wastewater</a> </p> <a href="https://publications.waset.org/abstracts/65699/a-sensitive-approach-on-trace-analysis-of-methylparaben-in-wastewater-and-cosmetic-products-using-molecularly-imprinted-polymer" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/65699.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">319</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">126</span> Direct Electrical Communication of Redox Enzyme Based on 3-Dimensional Crosslinked Redox Enzyme/Carbon Nanotube on a Thiol-Modified Au Surface</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=A.%20K.%20M.%20Kafi">A. K. M. Kafi</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20N.%20Nina"> S. N. Nina</a>, <a href="https://publications.waset.org/abstracts/search?q=Mashitah%20M.%20Yusoff"> Mashitah M. Yusoff</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In this work, we have described a new 3-dimensional (3D) network of crosslinked Horseradish Peroxidase/Carbon Nanotube (HRP/CNT) on a thiol-modified Au surface in order to build up the effective electrical wiring of the enzyme units with the electrode. This was achieved by the electropolymerization of aniline-functionalized carbon nanotubes (CNTs) and 4-aminothiophenol -modified-HRP on a 4-aminothiophenol monolayer-modified Au electrode. The synthesized 3D HRP/CNT networks were characterized with cyclic voltammetry and amperometry, resulting the establishment direct electron transfer between the redox active unit of HRP and the Au surface. Electrochemical measurements reveal that the immobilized HRP exhibits high biological activity and stability and a quasi-reversible redox peak of the redox center of HRP was observed at about −0.355 and −0.275 V vs. Ag/AgCl. The electron transfer rate constant, KS and electron transfer co-efficient were found to be 0.57 s-1 and 0.42, respectively. Based on the electrocatalytic process by direct electrochemistry of HRP, a biosensor for detecting H2O2 was developed. The developed biosensor exhibits excellent electrocatalytic activity for the reduction of H2O2. The proposed biosensor modified with HRP/CNT 3D network displays a broader linear range and a lower detection limit for H2O2 determination. The linear range is from 1.0×10−7 to 1.2×10−4M with a detection limit of 2.2.0×10−8M at 3σ. Moreover, this biosensor exhibits very high sensitivity, good reproducibility and long-time stability. In summary, ease of fabrication, a low cost, fast response and high sensitivity are the main advantages of the new biosensor proposed in this study. These obvious advantages would really help for the real analytical applicability of the proposed biosensor. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=biosensor" title="biosensor">biosensor</a>, <a href="https://publications.waset.org/abstracts/search?q=nanomaterials" title=" nanomaterials"> nanomaterials</a>, <a href="https://publications.waset.org/abstracts/search?q=redox%20enzyme" title=" redox enzyme"> redox enzyme</a>, <a href="https://publications.waset.org/abstracts/search?q=thiol-modified%20Au%20surface" title=" thiol-modified Au surface"> thiol-modified Au surface</a> </p> <a href="https://publications.waset.org/abstracts/16147/direct-electrical-communication-of-redox-enzyme-based-on-3-dimensional-crosslinked-redox-enzymecarbon-nanotube-on-a-thiol-modified-au-surface" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/16147.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">329</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">125</span> An Efficient Aptamer-Based Biosensor Developed via Irreversible Pi-Pi Functionalisation of Graphene/Zinc Oxide Nanocomposite</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sze%20Shin%20Low">Sze Shin Low</a>, <a href="https://publications.waset.org/abstracts/search?q=Michelle%20T.%20T.%20Tan"> Michelle T. T. Tan</a>, <a href="https://publications.waset.org/abstracts/search?q=Poi%20Sim%20Khiew"> Poi Sim Khiew</a>, <a href="https://publications.waset.org/abstracts/search?q=Hwei-San%20Loh"> Hwei-San Loh</a> </p> <p class="card-text"><strong>Abstract:</strong></p> An efficient graphene/zinc oxide (PSE-G/ZnO) platform based on pi-pi stacking, non-covalent interactions for the development of aptamer-based biosensor was presented in this study. As a proof of concept, the DNA recognition capability of the as-developed PSE-G/ZnO enhanced aptamer-based biosensor was evaluated using Coconut Cadang-cadang viroid disease (CCCVd). The G/ZnO nanocomposite was synthesised via a simple, green and efficient approach. The pristine graphene was produced through a single step exfoliation of graphite in sonochemical alcohol-water treatment while the zinc nitrate hexahydrate was mixed with the graphene and subjected to low temperature hydrothermal growth. The developed facile, environmental friendly method provided safer synthesis procedure by eliminating the need of harsh reducing chemicals and high temperature. The as-prepared nanocomposite was characterised by X-ray diffractometry (XRD), scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS) to evaluate its crystallinity, morphology and purity. Electrochemical impedance spectroscopy (EIS) was employed for the detection of CCCVd sequence with the use of potassium ferricyanide (K3[Fe(CN)6]). Recognition of the RNA analytes was achieved via the significant increase in resistivity for the double stranded DNA, as compared to single-stranded DNA. The PSE-G/ZnO enhanced aptamer-based biosensor exhibited higher sensitivity than the bare biosensor, attributing to the synergistic effect of high electrical conductivity of graphene and good electroactive property of ZnO. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=aptamer-based%20biosensor" title="aptamer-based biosensor">aptamer-based biosensor</a>, <a href="https://publications.waset.org/abstracts/search?q=graphene%2Fzinc%20oxide%20nanocomposite" title=" graphene/zinc oxide nanocomposite"> graphene/zinc oxide nanocomposite</a>, <a href="https://publications.waset.org/abstracts/search?q=green%20synthesis" title=" green synthesis"> green synthesis</a>, <a href="https://publications.waset.org/abstracts/search?q=screen%20printed%20carbon%20electrode" title=" screen printed carbon electrode"> screen printed carbon electrode</a> </p> <a href="https://publications.waset.org/abstracts/29610/an-efficient-aptamer-based-biosensor-developed-via-irreversible-pi-pi-functionalisation-of-graphenezinc-oxide-nanocomposite" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/29610.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">369</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">124</span> Localized Detection of ᴅ-Serine by Using an Enzymatic Amperometric Biosensor and Scanning Electrochemical Microscopy</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=David%20Polcari">David Polcari</a>, <a href="https://publications.waset.org/abstracts/search?q=Samuel%20C.%20Perry"> Samuel C. Perry</a>, <a href="https://publications.waset.org/abstracts/search?q=Loredano%20Pollegioni"> Loredano Pollegioni</a>, <a href="https://publications.waset.org/abstracts/search?q=Matthias%20Geissler"> Matthias Geissler</a>, <a href="https://publications.waset.org/abstracts/search?q=Janine%20Mauzeroll"> Janine Mauzeroll</a> </p> <p class="card-text"><strong>Abstract:</strong></p> ᴅ-serine acts as an endogenous co-agonist for N-methyl-ᴅ-aspartate receptors in neuronal synapses. This makes it a key component in the development and function of a healthy brain, especially given its role in several neurodegenerative diseases such as Alzheimer’s disease and dementia. Despite such clear research motivations, the primary site and mechanism of ᴅ-serine release is still currently unclear. For this reason, we are developing a biosensor for the detection of ᴅ-serine utilizing a microelectrode in combination with a ᴅ-amino acid oxidase enzyme, which produces stoichiometric quantities of hydrogen peroxide in response to ᴅ-serine. For the fabrication of a biosensor with good selectivity, we use a permselective poly(meta-phenylenediamine) film to ensure only the target molecule is reacted, according to the size exclusion principle. In this work, we investigated the effect of the electrodeposition conditions used on the biosensor’s response time and selectivity. Careful optimization of the fabrication process allowed for enhanced biosensor response time. This allowed for the real time sensing of ᴅ-serine in a bulk solution, and also provided in means to map the efflux of ᴅ-serine in real time. This was done using scanning electrochemical microscopy (SECM) with the optimized biosensor to measure localized release of ᴅ-serine from an agar filled glass capillary sealed in an epoxy puck, which acted as a model system. The SECM area scan simultaneously provided information regarding the rate of ᴅ-serine flux from the model substrate, as well as the size of the substrate itself. This SECM methodology, which provides high spatial and temporal resolution, could be useful to investigate the primary site and mechanism of ᴅ-serine release in other biological samples. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=%E1%B4%85-serine" title="ᴅ-serine">ᴅ-serine</a>, <a href="https://publications.waset.org/abstracts/search?q=enzymatic%20biosensor" title=" enzymatic biosensor"> enzymatic biosensor</a>, <a href="https://publications.waset.org/abstracts/search?q=microelectrode" title=" microelectrode"> microelectrode</a>, <a href="https://publications.waset.org/abstracts/search?q=scanning%20electrochemical%20microscopy" title=" scanning electrochemical microscopy"> scanning electrochemical microscopy</a> </p> <a href="https://publications.waset.org/abstracts/72424/localized-detection-of-serine-by-using-an-enzymatic-amperometric-biosensor-and-scanning-electrochemical-microscopy" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/72424.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">228</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">123</span> Development of Lectin-Based Biosensor for Glycoprofiling of Clinical Samples: Focus on Prostate Cancer</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Dominika%20Pihikova">Dominika Pihikova</a>, <a href="https://publications.waset.org/abstracts/search?q=Stefan%20Belicky"> Stefan Belicky</a>, <a href="https://publications.waset.org/abstracts/search?q=Tomas%20Bertok"> Tomas Bertok</a>, <a href="https://publications.waset.org/abstracts/search?q=Roman%20Sokol"> Roman Sokol</a>, <a href="https://publications.waset.org/abstracts/search?q=Petra%20Kubanikova"> Petra Kubanikova</a>, <a href="https://publications.waset.org/abstracts/search?q=Jan%20Tkac"> Jan Tkac</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Since aberrant glycosylation is frequently accompanied by both physiological and pathological processes in a human body (cancer, AIDS, inflammatory diseases, etc.), the analysis of tumor-associated glycan patterns have a great potential for the development of novel diagnostic approaches. Moreover, altered glycoforms may assist as a suitable tool for the specificity and sensitivity enhancement in early-stage prostate cancer diagnosis. In this paper we discuss the construction and optimization of ultrasensitive sandwich biosensor platform employing lectin as glycan-binding protein. We focus on the immunoassay development, reduction of non-specific interactions and final glycoprofiling of human serum samples including both prostate cancer (PCa) patients and healthy controls. The fabricated biosensor was measured by label-free electrochemical impedance spectroscopy (EIS) with further lectin microarray verification. Furthermore, we analyzed different biosensor interfaces with atomic force microscopy (AFM) in nanomechanical mapping mode showing a significant differences in the altitude. These preliminary results revealing an elevated content of α-2,3 linked sialic acid in PCa patients comparing with healthy controls. All these experiments are important step towards development of point-of-care devices and discovery of novel glyco-biomarkers applicable in cancer diagnosis. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=biosensor" title="biosensor">biosensor</a>, <a href="https://publications.waset.org/abstracts/search?q=glycan" title=" glycan"> glycan</a>, <a href="https://publications.waset.org/abstracts/search?q=lectin" title=" lectin"> lectin</a>, <a href="https://publications.waset.org/abstracts/search?q=prostate%20cancer" title=" prostate cancer"> prostate cancer</a> </p> <a href="https://publications.waset.org/abstracts/33642/development-of-lectin-based-biosensor-for-glycoprofiling-of-clinical-samples-focus-on-prostate-cancer" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/33642.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">372</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">122</span> Standardization of a Methodology for Quantification of Antimicrobials Used for the Treatment of Multi-Resistant Bacteria Using Two Types of Biosensors and Production of Anti-Antimicrobial Antibodies</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Garzon%20V.">Garzon V.</a>, <a href="https://publications.waset.org/abstracts/search?q=Bustos%20R."> Bustos R.</a>, <a href="https://publications.waset.org/abstracts/search?q=Salvador%20J.%20P."> Salvador J. P.</a>, <a href="https://publications.waset.org/abstracts/search?q=Marco%20M.%20P."> Marco M. P.</a>, <a href="https://publications.waset.org/abstracts/search?q=Pinacho%20D.%20G."> Pinacho D. G.</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Bacterial resistance to antimicrobial treatment has increased significantly in recent years, making it a public health problem. Large numbers of bacteria are resistant to all or nearly all known antimicrobials, creating the need for the development of new types of antimicrobials or the use of “last line” antimicrobial drug therapies for the treatment of multi-resistant bacteria. Some of the chemical groups of antimicrobials most used for the treatment of infections caused by multiresistant bacteria in the clinic are Glycopeptide (Vancomycin), Polymyxin (Colistin), Lipopeptide (Daptomycin) and Carbapenem (Meropenem). Molecules that require therapeutic drug monitoring (TDM). Due to the above, a methodology based on nanobiotechnology based on an optical and electrochemical biosensor is being developed, which allows the evaluation of the plasmatic levels of some antimicrobials such as glycopeptide, polymyxin, lipopeptide and carbapenem quickly, at a low cost, with a high specificity and sensitivity and that can be implemented in the future in public and private health hospitals. For this, the project was divided into five steps i) Design of specific anti-drug antibodies, produced in rabbits for each of the types of antimicrobials, evaluating the results by means of an immunoassay analysis (ELISA); ii) quantification by means of an electrochemical biosensor that allows quantification with high sensitivity and selectivity of the reference antimicrobials; iii) Comparison of antimicrobial quantification with an optical type biosensor; iv) Validation of the methodologies used with biosensor by means of an immunoassay. Finding as a result that it is possible to quantify antibiotics by means of the optical and electrochemical biosensor at concentrations on average of 1,000ng/mL, the antibodies being sensitive and specific for each of the antibiotic molecules, results that were compared with immunoassays and HPLC chromatography. Thus, contributing to the safe use of these drugs commonly used in clinical practice and new antimicrobial drugs. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=antibiotics" title="antibiotics">antibiotics</a>, <a href="https://publications.waset.org/abstracts/search?q=electrochemical%20biosensor" title=" electrochemical biosensor"> electrochemical biosensor</a>, <a href="https://publications.waset.org/abstracts/search?q=optical%20biosensor" title=" optical biosensor"> optical biosensor</a>, <a href="https://publications.waset.org/abstracts/search?q=therapeutic%20drug%20monitoring" title=" therapeutic drug monitoring"> therapeutic drug monitoring</a> </p> <a href="https://publications.waset.org/abstracts/163528/standardization-of-a-methodology-for-quantification-of-antimicrobials-used-for-the-treatment-of-multi-resistant-bacteria-using-two-types-of-biosensors-and-production-of-anti-antimicrobial-antibodies" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/163528.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">82</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">121</span> Direct Electrical Communication of Redox Enzyme Based on 3-Dimensional Cross-Linked Redox Enzyme/Nanomaterials</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=A.%20K.%20M.%20Kafi">A. K. M. Kafi</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20N.%20Nina"> S. N. Nina</a>, <a href="https://publications.waset.org/abstracts/search?q=Mashitah%20M.%20Yusoff"> Mashitah M. Yusoff</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In this work, we have described a new 3-dimensional (3D) network of cross-linked Horseradish Peroxidase/Carbon Nanotube (HRP/CNT) on a thiol-modified Au surface in order to build up the effective electrical wiring of the enzyme units with the electrode. This was achieved by the electropolymerization of aniline-functionalized carbon nanotubes (CNTs) and 4-aminothiophenol -modified-HRP on a 4-aminothiophenol monolayer-modified Au electrode. The synthesized 3D HRP/CNT networks were characterized with cyclic voltammetry and amperometry, resulting the establishment direct electron transfer between the redox active unit of HRP and the Au surface. Electrochemical measurements reveal that the immobilized HRP exhibits high biological activity and stability and a quasi-reversible redox peak of the redox center of HRP was observed at about −0.355 and −0.275 V vs. Ag/AgCl. The electron transfer rate constant, KS and electron transfer co-efficient were found to be 0.57 s-1 and 0.42, respectively. Based on the electrocatalytic process by direct electrochemistry of HRP, a biosensor for detecting H2O2 was developed. The developed biosensor exhibits excellent electrocatalytic activity for the reduction of H2O2. The proposed biosensor modified with HRP/CNT 3D network displays a broader linear range and a lower detection limit for H2O2 determination. The linear range is from 1.0×10−7 to 1.2×10−4M with a detection limit of 2.2.0×10−8M at 3σ. Moreover, this biosensor exhibits very high sensitivity, good reproducibility and long-time stability. In summary, ease of fabrication, a low cost, fast response and high sensitivity are the main advantages of the new biosensor proposed in this study. These obvious advantages would really help for the real analytical applicability of the proposed biosensor. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=redox%20enzyme" title="redox enzyme">redox enzyme</a>, <a href="https://publications.waset.org/abstracts/search?q=nanomaterials" title=" nanomaterials"> nanomaterials</a>, <a href="https://publications.waset.org/abstracts/search?q=biosensors" title=" biosensors"> biosensors</a>, <a href="https://publications.waset.org/abstracts/search?q=electrical%20communication" title=" electrical communication"> electrical communication</a> </p> <a href="https://publications.waset.org/abstracts/24276/direct-electrical-communication-of-redox-enzyme-based-on-3-dimensional-cross-linked-redox-enzymenanomaterials" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/24276.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">454</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">120</span> Sensitivity Enhancement of Photonic Crystal Fiber Biosensor </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mohamed%20Farhat%20O.%20Hameed">Mohamed Farhat O. Hameed</a>, <a href="https://publications.waset.org/abstracts/search?q=Yasamin%20K.%20A.%20Alrayk"> Yasamin K. A. Alrayk</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20A%20Shaalan"> A. A Shaalan</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20S.%20A.%20Obayya"> S. S. A. Obayya</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The surface plasmon resonance (SPR) sensors are widely used due to its high sensitivity with molecular labels free. The commercial SPR sensors depend on the conventional prism-coupled configuration. However, this type of configuration suffers from miniaturization and integration. Therefore, the search for compact, portable and highly sensitive SPR sensors becomes mandatory.In this paper, sensitivity enhancement of a novel photonic crystal fiber biosensoris introduced and studied. The suggested design has microstructure of air holes in the core region surrounded by two large semicircular metallized channels filled with the analyte. The inner surfaces of the two channels are coated by a silver layer followed by a gold layer.The simulation results are obtained using full vectorial finite element methodwith perfect matched layer (PML) boundary conditions. The proposed design depends on bimetallic configuration to enhance the biosensor sensitivity. Additionally, the suggested biosensor can be used for multi-channel/multi-analyte sensing. In this study, the sensor geometrical parameters are studied to maximize the sensitivity for the two polarized modes. The numerical results show that high refractive index sensitivity of 4750 nm/RIU (refractive index unit) and 4300 nm/RIU can be achieved for the quasi (transverse magnetic) TM and quasi (transverse electric) TE modes of the proposed biosensor, respectively. The reportedbiosensor has advantages of integration of microfluidics setup, waveguide and metallic layers into a single structure. As a result, compact biosensor with better integration compared to conventional optical fiber SPR biosensors can be obtained. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=photonic%20crystal%20fibers" title="photonic crystal fibers">photonic crystal fibers</a>, <a href="https://publications.waset.org/abstracts/search?q=gold" title=" gold"> gold</a>, <a href="https://publications.waset.org/abstracts/search?q=silver" title=" silver"> silver</a>, <a href="https://publications.waset.org/abstracts/search?q=surface%20plasmon" title=" surface plasmon"> surface plasmon</a>, <a href="https://publications.waset.org/abstracts/search?q=biosensor" title=" biosensor"> biosensor</a> </p> <a href="https://publications.waset.org/abstracts/40173/sensitivity-enhancement-of-photonic-crystal-fiber-biosensor" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/40173.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">380</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">119</span> Modified Poly (Pyrrole) Film-Based Biosensors for Phenol Detection</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=S.%20Korkut">S. Korkut</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20S.%20Kilic"> M. S. Kilic</a>, <a href="https://publications.waset.org/abstracts/search?q=E.%20Erhan"> E. Erhan</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In order to detect and quantify the phenolic contents of a wastewater with biosensors, two working electrodes based on modified Poly (Pyrrole) films were fabricated. Enzyme horseradish peroxidase was used as biomolecule of the prepared electrodes. Various phenolics were tested at the biosensor. Phenol detection was realized by electrochemical reduction of quinones produced by enzymatic activity. Analytical parameters were calculated and the results were compared with each other. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=carbon%20nanotube" title="carbon nanotube">carbon nanotube</a>, <a href="https://publications.waset.org/abstracts/search?q=phenol%20biosensor" title=" phenol biosensor"> phenol biosensor</a>, <a href="https://publications.waset.org/abstracts/search?q=polypyrrole" title=" polypyrrole"> polypyrrole</a>, <a href="https://publications.waset.org/abstracts/search?q=poly%20%28glutaraldehyde%29" title=" poly (glutaraldehyde)"> poly (glutaraldehyde)</a> </p> <a href="https://publications.waset.org/abstracts/22105/modified-poly-pyrrole-film-based-biosensors-for-phenol-detection" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/22105.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">419</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">118</span> Impedance Based Biosensor for Agricultural Pathogen Detection</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Rhea%20Patel">Rhea Patel</a>, <a href="https://publications.waset.org/abstracts/search?q=Madhuri%20Vinchurkar"> Madhuri Vinchurkar</a>, <a href="https://publications.waset.org/abstracts/search?q=Rajul%20Patkar"> Rajul Patkar</a>, <a href="https://publications.waset.org/abstracts/search?q=Gopal%20Pranjale"> Gopal Pranjale</a>, <a href="https://publications.waset.org/abstracts/search?q=Maryam%20Shojaei%20Baghini"> Maryam Shojaei Baghini</a> </p> <p class="card-text"><strong>Abstract:</strong></p> One of the major limitations on food resources worldwide is the deterioration of plant products due to pathogenic infections. Early screening of plants for pathogenic infections can serve as a boon in the Agricultural sector. The standard microbiology techniques has not kept pace with the rapid enumeration and automated methods for bacteria detection. Electrochemical Impedance Spectroscopy (EIS) serves as a label free bio sensing technique to monitor pathogens in real time. The changes in the electrical impedance of a growing bacterial culture can be monitored to detect activity of microorganisms. In this study, we demonstrate development of a gold interdigitated electrode (gold IDE) based impedance biosensor to detect bacterial cells in real on-field crop samples. To calibrate our impedance measurement system, nutrient broth suspended Escherichia coli cells were used. We extended this calibrated protocol to identify the agricultural pathogens in real potato tuber samples. Distinct difference was seen in the impedance recorded for the healthy and infected potato samples. Our results support the potential application of this Impedance based biosensor in Agricultural pathogen detection. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=agriculture" title="agriculture">agriculture</a>, <a href="https://publications.waset.org/abstracts/search?q=biosensor" title=" biosensor"> biosensor</a>, <a href="https://publications.waset.org/abstracts/search?q=electrochemical%20impedance%20spectroscopy" title=" electrochemical impedance spectroscopy"> electrochemical impedance spectroscopy</a>, <a href="https://publications.waset.org/abstracts/search?q=microelectrode" title=" microelectrode"> microelectrode</a>, <a href="https://publications.waset.org/abstracts/search?q=pathogen%20detection" title=" pathogen detection"> pathogen detection</a> </p> <a href="https://publications.waset.org/abstracts/129796/impedance-based-biosensor-for-agricultural-pathogen-detection" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/129796.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">155</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">117</span> A DNA-Based Nano-biosensor for the Rapid Detection of the Dengue Virus in Mosquito </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Lilia%20M.%20Fernando">Lilia M. Fernando</a>, <a href="https://publications.waset.org/abstracts/search?q=Matthew%20K.%20Vasher"> Matthew K. Vasher</a>, <a href="https://publications.waset.org/abstracts/search?q=Evangelyn%20C.%20Alocilja"> Evangelyn C. Alocilja</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This paper describes the development of a DNA-based nanobiosensor to detect the dengue virus in mosquito using electrically active magnetic (EAM) nanoparticles as the concentrator and electrochemical transducer. The biosensor detection encompasses two sets of oligonucleotide probes that are specific to the dengue virus: the detector probe labeled with the EAM nanoparticles and the biotinylated capture probe. The DNA targets are double hybridized to the detector and the capture probes and concentrated from nonspecific DNA fragments by applying a magnetic field. Subsequently, the DNA sandwiched targets (EAM-detector probe–DNA target–capture probe-biotin) are captured on streptavidin modified screen printed carbon electrodes through the biotinylated capture probes. Detection is achieved electrochemically by measuring the oxidation–reduction signal of the EAM nanoparticles. Results indicate that the biosensor is able to detect the redox signal of the EAM nanoparticles at dengue DNA concentrations as low as 10 ng/ul. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=dengue" title="dengue">dengue</a>, <a href="https://publications.waset.org/abstracts/search?q=magnetic%20nanoparticles" title=" magnetic nanoparticles"> magnetic nanoparticles</a>, <a href="https://publications.waset.org/abstracts/search?q=mosquito" title=" mosquito"> mosquito</a>, <a href="https://publications.waset.org/abstracts/search?q=nanobiosensor" title=" nanobiosensor"> nanobiosensor</a> </p> <a href="https://publications.waset.org/abstracts/37690/a-dna-based-nano-biosensor-for-the-rapid-detection-of-the-dengue-virus-in-mosquito" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/37690.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">366</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">116</span> Biosensors for Parathion Based on Au-Pd Nanoparticles Modified Electrodes</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Tian-Fang%20Kang">Tian-Fang Kang</a>, <a href="https://publications.waset.org/abstracts/search?q=Chao-Nan%20Ge"> Chao-Nan Ge</a>, <a href="https://publications.waset.org/abstracts/search?q=Rui%20Li"> Rui Li</a> </p> <p class="card-text"><strong>Abstract:</strong></p> An electrochemical biosensor for the determination of organophosphorus pesticides was developed based on electrochemical co-deposition of Au and Pd nanoparticles on glassy carbon electrode (GCE). Energy disperse spectroscopy (EDS) analysis was used for characterization of the surface structure. Scanning electron micrograph (SEM) demonstrates that the films are uniform and the nanoclusters are homogeneously distributed on the GCE surface. Acetylcholinesterase (AChE) was immobilized on the Au and Pd nanoparticle modified electrode (Au-Pd/GCE) by cross-linking with glutaraldehyde. The electrochemical behavior of thiocholine at the biosensor (AChE/Au-Pd/GCE) was studied. The biosensors exhibited substantial electrocatalytic effect on the oxidation of thiocholine. The peak current of linear scan voltammetry (LSV) of thiocholine at the biosensor is proportional to the concentration of acetylthiocholine chloride (ATCl) over the range of 2.5 × 10-6 to 2.5 × 10-4 M in 0.1 M phosphate buffer solution (pH 7.0). The percent inhibition of acetylcholinesterase was proportional to the logarithm of parathion concentration in the range of 4.0 × 10-9 to 1.0 × 10-6 M. The detection limit of parathion was 2.6 × 10-9 M. The proposed method exhibited high sensitivity and good reproducibility. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=acetylcholinesterase" title="acetylcholinesterase">acetylcholinesterase</a>, <a href="https://publications.waset.org/abstracts/search?q=Au-Pd%20nanoparticles" title=" Au-Pd nanoparticles"> Au-Pd nanoparticles</a>, <a href="https://publications.waset.org/abstracts/search?q=electrochemical%20biosensors" title=" electrochemical biosensors"> electrochemical biosensors</a>, <a href="https://publications.waset.org/abstracts/search?q=parathion" title=" parathion "> parathion </a> </p> <a href="https://publications.waset.org/abstracts/17756/biosensors-for-parathion-based-on-au-pd-nanoparticles-modified-electrodes" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/17756.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">407</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">115</span> Assessment of Highly Sensitive Dielectric Modulated GaN-FinFET for Label-Free Biosensing Applications</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ajay%20Kumar">Ajay Kumar</a>, <a href="https://publications.waset.org/abstracts/search?q=Neha%20Gupta"> Neha Gupta</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This work presents the sensitivity assessment of Gallium Nitride (GaN) material-based FinFET by dielectric modulation in the nanocavity gap for label-free biosensing applications. The significant deflection is observed in the electrical characteristics such as drain current (ID), transconductance (gm), surface potential, energy band profile, electric field, sub-threshold slope (SS), and threshold voltage (Vth) in the presence of biomolecules owing to GaN material. Further, the device sensitivity is evaluated to identify the effectiveness of the proposed biosensor and its capability to detect the biomolecules with high precision or accuracy. Higher sensitivity is observed for Gelatin (k=12) in terms of on-current (SION), threshold voltage (SVth), and switching ratio (SSR) by 104.88%, 82.12%, and 119.73%, respectively. This work is performed using a powerful tool 3D Sentaurus TCAD using a well-calibrated structure. All the results pave the way for GaN-FinFET as a viable candidate for label-free dielectric modulated biosensor applications. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=biosensor" title="biosensor">biosensor</a>, <a href="https://publications.waset.org/abstracts/search?q=biomolecules" title=" biomolecules"> biomolecules</a>, <a href="https://publications.waset.org/abstracts/search?q=FinFET" title=" FinFET"> FinFET</a>, <a href="https://publications.waset.org/abstracts/search?q=sensitivity" title=" sensitivity"> sensitivity</a> </p> <a href="https://publications.waset.org/abstracts/153336/assessment-of-highly-sensitive-dielectric-modulated-gan-finfet-for-label-free-biosensing-applications" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/153336.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">204</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">114</span> Peptide Aptasensor for Electrochemical Detection of Rheumatoid Arthritis </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Shah%20Abbas">Shah Abbas</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Rheumatoid arthritis is a systemic, inflammatory autoimmune disease, affecting an overall 1% of the global population. Despite being tremendous efforts by scientists, early diagnosis of RA still has not been achieved. In the current study, a Graphene oxide (GO) based electrochemical sensor has been developed for early diagnosis of RA through Cyclic voltammetry. Chitosan (CHI), a CPnatural polymer has also been incorporated along with GO in order to enhance the biocompatibility and functionalization potential of the biosensor. CCPs are known antigens for Anti Citrullinated Peptide Antibodies (ACPAs) which can be detected in serum even 14 years before the appearance of symptoms, thus they are believed to be an ideal target for the early diagnosis of RA. This study has yielded some promising results regarding the binding and detection of ACPAs through changes in the electrochemical properties of biosensing material. The cyclic voltammogram of this biosensor reflects the binding of ACPAs to the biosensor surface, due to its shifts observed in the current flow (cathodic current) as compared to the when no ACPAs bind as it is absent in RA negative patients. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=rheumatoid%20arthritis" title="rheumatoid arthritis">rheumatoid arthritis</a>, <a href="https://publications.waset.org/abstracts/search?q=peptide%20sensor" title=" peptide sensor"> peptide sensor</a>, <a href="https://publications.waset.org/abstracts/search?q=graphene%20oxide" title=" graphene oxide"> graphene oxide</a>, <a href="https://publications.waset.org/abstracts/search?q=anti%20citrullinated%20peptide%20antibodies" title=" anti citrullinated peptide antibodies"> anti citrullinated peptide antibodies</a>, <a href="https://publications.waset.org/abstracts/search?q=cyclic%20voltammetry" title=" cyclic voltammetry"> cyclic voltammetry</a> </p> <a href="https://publications.waset.org/abstracts/125129/peptide-aptasensor-for-electrochemical-detection-of-rheumatoid-arthritis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/125129.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">142</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">113</span> Quantum Dot Biosensing for Advancing Precision Cancer Detection</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sourav%20Sarkar">Sourav Sarkar</a>, <a href="https://publications.waset.org/abstracts/search?q=Manashjit%20Gogoi"> Manashjit Gogoi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In the evolving landscape of cancer diagnostics, optical biosensing has emerged as a promising tool due to its sensitivity and specificity. This study explores the potential of CdS/ZnS core-shell quantum dots (QDs) capped with 3-Mercaptopropionic acid (3-MPA), which aids in the linking chemistry of QDs to various cancer antibodies. The QDs, with their unique optical and electronic properties, have been integrated into the biosensor design. Their high quantum yield and size-dependent emission spectra have been exploited to improve the sensor’s detection capabilities. The study presents the design of this QD-enhanced optical biosensor. The use of these QDs can also aid multiplexed detection, enabling simultaneous monitoring of different cancer biomarkers. This innovative approach holds significant potential for advancing cancer diagnostics, contributing to timely and accurate detection. Future work will focus on optimizing the biosensor design for clinical applications and exploring the potential of QDs in other biosensing applications. This study underscores the potential of integrating nanotechnology and biosensing for cancer research, paving the way for next-generation diagnostic tools. It is a step forward in our quest for achieving precision oncology. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=quantum%20dots" title="quantum dots">quantum dots</a>, <a href="https://publications.waset.org/abstracts/search?q=biosensing" title=" biosensing"> biosensing</a>, <a href="https://publications.waset.org/abstracts/search?q=cancer" title=" cancer"> cancer</a>, <a href="https://publications.waset.org/abstracts/search?q=device" title=" device"> device</a> </p> <a href="https://publications.waset.org/abstracts/179627/quantum-dot-biosensing-for-advancing-precision-cancer-detection" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/179627.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">56</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">112</span> Ambient Electrospray Deposition: An Efficient Technique to Immobilize Laccase on Cheap Electrodes With Unprecedented Reuse and Storage Performances</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mattea%20Carmen%20Castrovilli">Mattea Carmen Castrovilli</a>, <a href="https://publications.waset.org/abstracts/search?q=Antonella%20Cartoni"> Antonella Cartoni</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Electrospray ionisation (ESI), a well-established technique widely used to produce ion beams of biomolecules in mass spectrometry (ESI-MS), can be used for ambient soft landing of enzymes on a specific substrate. In this work, we show how the ambient electrospray deposition (ESD) technique can be successfully exploited for manufacturing a promising, green-friendly electrochemical amperometric laccase-based biosensor with unprecedented reuse and storage performance. These biosensors have been manufactured by spraying a laccase solution of 2μg/μL at 20% of methanol on a commercial carbon screen printed electrode (C-SPE) using a custom ESD set-up. The laccase-based ESD biosensor has been tested against catechol compounds in the linear range 2-100 μM, with a limit of detection of 1.7 μM, without interference from cadmium, chrome, arsenic, and zinc and without any memory effects, but showing a matrix effect in lake and well water. The ESD biosensor shows enhanced performances compared to the ones fabricated with other immobilization methods, like drop-casting. Indeed, it retains 100% activity up to two months of storage at ambient conditions without any special care and working stability up to 63 measurements on the same electrode just prepared and 20 on a one-year-old electrode subjected to redeposition together with a 100% resistance to use of the same electrode in subsequent days. The ESD method is a one-step, environmentally friendly method that allows the deposition of the bio-recognition layer without using any additional chemicals. The promising results in terms of storage and working stability also obtained with the more fragile lactate oxidase enzyme suggest these improvements should be attributed to the ESD technique rather than to the bioreceptor, highlighting how the ESD could be useful in reducing pollution from disposable devices. Acknowledgment: The understanding at the molecular level of this promising biosensor by using different spectroscopies, microscopies and analytical techniques is the subject of our PRIN 2022 project ESILARANTE. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=reuse" title="reuse">reuse</a>, <a href="https://publications.waset.org/abstracts/search?q=storage%20performance" title=" storage performance"> storage performance</a>, <a href="https://publications.waset.org/abstracts/search?q=immobilization" title=" immobilization"> immobilization</a>, <a href="https://publications.waset.org/abstracts/search?q=electrospray%20deposition" title=" electrospray deposition"> electrospray deposition</a>, <a href="https://publications.waset.org/abstracts/search?q=biosensor" title=" biosensor"> biosensor</a>, <a href="https://publications.waset.org/abstracts/search?q=laccase" title=" laccase"> laccase</a>, <a href="https://publications.waset.org/abstracts/search?q=catechol%20detection" title=" catechol detection"> catechol detection</a>, <a href="https://publications.waset.org/abstracts/search?q=green%20chemistry" title=" green chemistry"> green chemistry</a> </p> <a href="https://publications.waset.org/abstracts/175637/ambient-electrospray-deposition-an-efficient-technique-to-immobilize-laccase-on-cheap-electrodes-with-unprecedented-reuse-and-storage-performances" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/175637.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">62</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">111</span> Characterization of the Dispersion Phenomenon in an Optical Biosensor</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=An-Shik%20Yang">An-Shik Yang</a>, <a href="https://publications.waset.org/abstracts/search?q=Chin-Ting%20Kuo"> Chin-Ting Kuo</a>, <a href="https://publications.waset.org/abstracts/search?q=Yung-Chun%20Yang"> Yung-Chun Yang</a>, <a href="https://publications.waset.org/abstracts/search?q=Wen-Hsin%20Hsieh"> Wen-Hsin Hsieh</a>, <a href="https://publications.waset.org/abstracts/search?q=Chiang-Ho%20Cheng"> Chiang-Ho Cheng</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Optical biosensors have become a powerful detection and analysis tool for wide-ranging applications in biomedical research, pharmaceuticals and environmental monitoring. This study carried out the computational fluid dynamics (CFD)-based simulations to explore the dispersion phenomenon in the microchannel of a optical biosensor. The predicted time sequences of concentration contours were utilized to better understand the dispersion development occurred in different geometric shapes of microchannels. The simulation results showed the surface concentrations at the sensing probe (with the best performance of a grating coupler) in respect of time to appraise the dispersion effect and therefore identify the design configurations resulting in minimum dispersion. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=CFD%20simulations" title="CFD simulations">CFD simulations</a>, <a href="https://publications.waset.org/abstracts/search?q=dispersion" title=" dispersion"> dispersion</a>, <a href="https://publications.waset.org/abstracts/search?q=microfluidic" title=" microfluidic"> microfluidic</a>, <a href="https://publications.waset.org/abstracts/search?q=optical%20waveguide%20sensors" title=" optical waveguide sensors"> optical waveguide sensors</a> </p> <a href="https://publications.waset.org/abstracts/24715/characterization-of-the-dispersion-phenomenon-in-an-optical-biosensor" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/24715.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">545</span> </span> </div> </div> <ul class="pagination"> <li class="page-item disabled"><span class="page-link">&lsaquo;</span></li> <li class="page-item active"><span class="page-link">1</span></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=biosensor&page=2">2</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=biosensor&page=3">3</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=biosensor&page=4">4</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=biosensor&page=5">5</a></li> <li class="page-item"><a class="page-link" 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