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overflow: hidden; text-overflow: ellipsis; -webkit-line-clamp: 3; -webkit-box-orient: vertical; }</style><div class="col-xs-12 clearfix"><div class="u-floatLeft"><h1 class="PageHeader-title u-m0x u-fs30">Chromatography</h1><div class="u-tcGrayDark">16,136 Followers</div><div class="u-tcGrayDark u-mt2x">Recent papers in <b>Chromatography</b></div></div></div></div></div></div><div class="TabbedNavigation"><div class="container"><div class="row"><div class="col-xs-12 clearfix"><ul class="nav u-m0x u-p0x list-inline u-displayFlex"><li class="active"><a href="https://www.academia.edu/Documents/in/Chromatography">Top Papers</a></li><li><a href="https://www.academia.edu/Documents/in/Chromatography/MostCited">Most Cited Papers</a></li><li><a href="https://www.academia.edu/Documents/in/Chromatography/MostDownloaded">Most Downloaded Papers</a></li><li><a href="https://www.academia.edu/Documents/in/Chromatography/MostRecent">Newest Papers</a></li><li><a class="" href="https://www.academia.edu/People/Chromatography">People</a></li></ul></div><style type="text/css">ul.nav{flex-direction:row}@media(max-width: 567px){ul.nav{flex-direction:column}.TabbedNavigation li{max-width:100%}.TabbedNavigation li.active{background-color:var(--background-grey, #dddde2)}.TabbedNavigation li.active:before,.TabbedNavigation li.active:after{display:none}}</style></div></div></div><div class="container"><div class="row"><div class="col-xs-12"><div class="u-displayFlex"><div class="u-flexGrow1"><div class="works"><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_76462641" data-work_id="76462641" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/76462641/Validation_and_clinical_application_of_a_novel_LC_MS_method_for_quantification_of_dolutegravir_in_breast_milk">Validation and clinical application of a novel LC–MS method for quantification of dolutegravir in breast milk</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Aim: A novel, sensitive and reproducible method for quantification of dolutegravir (DTG) in dried breast milk spots (DBMS) was developed and validated for use in clinical studies. Its application enabled measurement of DTG... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_76462641" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Aim: A novel, sensitive and reproducible method for quantification of dolutegravir (DTG) in dried breast milk spots (DBMS) was developed and validated for use in clinical studies. Its application enabled measurement of DTG pharmacokinetics in breastfeeding mothers and their infants. Results/methodology: Sample extraction was by liquid–liquid extraction using tert-butyl methy-ether, with DTG–d5 as an internal standard. DTG was eluted on a reverse phase C18 Waters XBridge (3.5 μm: 2.1 × 50 mm) column using a gradient mobile phase consisting of 0.1% formic acid in deionised water or methanol. The assay was validated over a calibration range of 10–4000 ng/ml. Conclusion: Stability, inter and intra-assay variability were acceptable according to FDA and EMA bioanalytical method guidelines. The assay is robust, accurate, precise and can be reliably applied for analysis of clinical samples in trials from low resource settings.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/76462641" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="e0d2c32909da073908fd0df874a4a2c1" rel="nofollow" data-download="{"attachment_id":84163422,"asset_id":76462641,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/84163422/download_file?st=MTczMjQwNDEzOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="24533322" href="https://liverpool.academia.edu/JoshuaGini">Joshua Gini</a><script data-card-contents-for-user="24533322" type="text/json">{"id":24533322,"first_name":"Joshua","last_name":"Gini","domain_name":"liverpool","page_name":"JoshuaGini","display_name":"Joshua Gini","profile_url":"https://liverpool.academia.edu/JoshuaGini?f_ri=4656","photo":"https://0.academia-photos.com/24533322/6625539/7488582/s65_joshua.gini.jpg_oh_1b8049b9d6e9a4a8f68f25134d20aa4a_oe_5526e2eb___gda___1433155159_83d0be6f7dd7c1f21848c4f083da3744"}</script></span></span></li><li class="js-paper-rank-work_76462641 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="76462641"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 76462641, container: ".js-paper-rank-work_76462641", }); 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$(".js-view-count[data-work-id=76462641]").text(description); $(".js-view-count-work_76462641").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_76462641").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="76462641"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">4</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="4656" href="https://www.academia.edu/Documents/in/Chromatography">Chromatography</a>, <script data-card-contents-for-ri="4656" type="text/json">{"id":4656,"name":"Chromatography","url":"https://www.academia.edu/Documents/in/Chromatography?f_ri=4656","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="6041" href="https://www.academia.edu/Documents/in/Bioanalysis">Bioanalysis</a>, <script data-card-contents-for-ri="6041" type="text/json">{"id":6041,"name":"Bioanalysis","url":"https://www.academia.edu/Documents/in/Bioanalysis?f_ri=4656","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="26327" href="https://www.academia.edu/Documents/in/Medicine">Medicine</a>, <script data-card-contents-for-ri="26327" type="text/json">{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine?f_ri=4656","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="3979951" href="https://www.academia.edu/Documents/in/Dolutegravir">Dolutegravir</a><script data-card-contents-for-ri="3979951" type="text/json">{"id":3979951,"name":"Dolutegravir","url":"https://www.academia.edu/Documents/in/Dolutegravir?f_ri=4656","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=76462641]'), work: {"id":76462641,"title":"Validation and clinical application of a novel LC–MS method for quantification of dolutegravir in breast milk","created_at":"2022-04-14T14:25:25.785-07:00","url":"https://www.academia.edu/76462641/Validation_and_clinical_application_of_a_novel_LC_MS_method_for_quantification_of_dolutegravir_in_breast_milk?f_ri=4656","dom_id":"work_76462641","summary":"Aim: A novel, sensitive and reproducible method for quantification of dolutegravir (DTG) in dried breast milk spots (DBMS) was developed and validated for use in clinical studies. Its application enabled measurement of DTG pharmacokinetics in breastfeeding mothers and their infants. Results/methodology: Sample extraction was by liquid–liquid extraction using tert-butyl methy-ether, with DTG–d5 as an internal standard. DTG was eluted on a reverse phase C18 Waters XBridge (3.5 μm: 2.1 × 50 mm) column using a gradient mobile phase consisting of 0.1% formic acid in deionised water or methanol. The assay was validated over a calibration range of 10–4000 ng/ml. Conclusion: Stability, inter and intra-assay variability were acceptable according to FDA and EMA bioanalytical method guidelines. The assay is robust, accurate, precise and can be reliably applied for analysis of clinical samples in trials from low resource settings.","downloadable_attachments":[{"id":84163422,"asset_id":76462641,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":24533322,"first_name":"Joshua","last_name":"Gini","domain_name":"liverpool","page_name":"JoshuaGini","display_name":"Joshua Gini","profile_url":"https://liverpool.academia.edu/JoshuaGini?f_ri=4656","photo":"https://0.academia-photos.com/24533322/6625539/7488582/s65_joshua.gini.jpg_oh_1b8049b9d6e9a4a8f68f25134d20aa4a_oe_5526e2eb___gda___1433155159_83d0be6f7dd7c1f21848c4f083da3744"}],"research_interests":[{"id":4656,"name":"Chromatography","url":"https://www.academia.edu/Documents/in/Chromatography?f_ri=4656","nofollow":false},{"id":6041,"name":"Bioanalysis","url":"https://www.academia.edu/Documents/in/Bioanalysis?f_ri=4656","nofollow":false},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine?f_ri=4656","nofollow":false},{"id":3979951,"name":"Dolutegravir","url":"https://www.academia.edu/Documents/in/Dolutegravir?f_ri=4656","nofollow":false}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_77643343" data-work_id="77643343" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/77643343/A_Comparison_of_the_Absorption_of_a_Series_of_Ethoxylates_through_Rat_Skin_In_Vitro">A Comparison of the Absorption of a Series of Ethoxylates through Rat Skin In Vitro</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/77643343" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="8d9e56999d6826f0602321a7d733ffd4" rel="nofollow" data-download="{"attachment_id":84957946,"asset_id":77643343,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/84957946/download_file?st=MTczMjQwNDEzOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="36742611" href="https://independent.academia.edu/PeterBlain">Peter Blain</a><script data-card-contents-for-user="36742611" type="text/json">{"id":36742611,"first_name":"Peter","last_name":"Blain","domain_name":"independent","page_name":"PeterBlain","display_name":"Peter Blain","profile_url":"https://independent.academia.edu/PeterBlain?f_ri=4656","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_77643343 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="77643343"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 77643343, container: ".js-paper-rank-work_77643343", }); 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$(".js-view-count[data-work-id=77643343]").text(description); $(".js-view-count-work_77643343").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_77643343").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="77643343"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">5</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="523" href="https://www.academia.edu/Documents/in/Chemistry">Chemistry</a>, <script data-card-contents-for-ri="523" type="text/json">{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry?f_ri=4656","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="4656" href="https://www.academia.edu/Documents/in/Chromatography">Chromatography</a>, <script data-card-contents-for-ri="4656" type="text/json">{"id":4656,"name":"Chromatography","url":"https://www.academia.edu/Documents/in/Chromatography?f_ri=4656","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="518679" href="https://www.academia.edu/Documents/in/In_Vitro_Toxicology">In Vitro Toxicology</a>, <script data-card-contents-for-ri="518679" type="text/json">{"id":518679,"name":"In Vitro Toxicology","url":"https://www.academia.edu/Documents/in/In_Vitro_Toxicology?f_ri=4656","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="897823" href="https://www.academia.edu/Documents/in/Elsevier">Elsevier</a><script data-card-contents-for-ri="897823" type="text/json">{"id":897823,"name":"Elsevier","url":"https://www.academia.edu/Documents/in/Elsevier?f_ri=4656","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=77643343]'), work: {"id":77643343,"title":"A Comparison of the Absorption of a Series of Ethoxylates through Rat Skin In Vitro","created_at":"2022-04-26T01:27:34.020-07:00","url":"https://www.academia.edu/77643343/A_Comparison_of_the_Absorption_of_a_Series_of_Ethoxylates_through_Rat_Skin_In_Vitro?f_ri=4656","dom_id":"work_77643343","summary":null,"downloadable_attachments":[{"id":84957946,"asset_id":77643343,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":36742611,"first_name":"Peter","last_name":"Blain","domain_name":"independent","page_name":"PeterBlain","display_name":"Peter Blain","profile_url":"https://independent.academia.edu/PeterBlain?f_ri=4656","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry?f_ri=4656","nofollow":false},{"id":4656,"name":"Chromatography","url":"https://www.academia.edu/Documents/in/Chromatography?f_ri=4656","nofollow":false},{"id":518679,"name":"In Vitro Toxicology","url":"https://www.academia.edu/Documents/in/In_Vitro_Toxicology?f_ri=4656","nofollow":false},{"id":897823,"name":"Elsevier","url":"https://www.academia.edu/Documents/in/Elsevier?f_ri=4656","nofollow":false},{"id":3789884,"name":"Pharmacology and pharmaceutical sciences","url":"https://www.academia.edu/Documents/in/Pharmacology_and_pharmaceutical_sciences?f_ri=4656"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_28244455" data-work_id="28244455" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/28244455/Removal_of_Endotoxin_from_Protein_in_Pharmaceutical_Processes">Removal of Endotoxin from Protein in Pharmaceutical Processes</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Bacterial endotoxin is the lipopolysaccharide component of the cell wall of Gram-negative bacteria, together with other cellular material that combines to form an endotoxin complex. Endotoxin is pyrogenic and it presents a risk to... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_28244455" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Bacterial endotoxin is the lipopolysaccharide component of the cell wall of Gram-negative bacteria, together with other cellular material that combines to form an endotoxin complex. Endotoxin is pyrogenic and it presents a risk to patients who are administered intravenous and intramuscular preparations.1Thus bacterial endotoxins pose a risk to many pharmaceutical processes and, where not controlled, to the finished products. There are different methods for endotoxin removal. These include depyrogenation,2 such as dry-heat processes applied to glassware, and rinsing,3 as might be applied to closures. These areas receive reasonable coverage within the pharmaceutical sector. Areas that are actively discussed within biotechnology fields but which receive less attention in the wider pharmaceutical context are steps to remove endotoxin bound to protein. This article considers some of the biotechnological applications for endotoxin removal.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/28244455" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="c9f6b62b2151988c33578110e34a1f9e" rel="nofollow" data-download="{"attachment_id":48564229,"asset_id":28244455,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/48564229/download_file?st=MTczMjQwNDEzOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="725124" href="https://manchester.academia.edu/TimSandle">Tim Sandle</a><script data-card-contents-for-user="725124" type="text/json">{"id":725124,"first_name":"Tim","last_name":"Sandle","domain_name":"manchester","page_name":"TimSandle","display_name":"Tim Sandle","profile_url":"https://manchester.academia.edu/TimSandle?f_ri=4656","photo":"https://0.academia-photos.com/725124/247895/293007/s65_tim.sandle.jpg"}</script></span></span></li><li class="js-paper-rank-work_28244455 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="28244455"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 28244455, container: ".js-paper-rank-work_28244455", }); 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$(".js-view-count[data-work-id=28244455]").text(description); $(".js-view-count-work_28244455").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_28244455").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="28244455"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">8</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="159" href="https://www.academia.edu/Documents/in/Microbiology">Microbiology</a>, <script data-card-contents-for-ri="159" type="text/json">{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=4656","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="656" href="https://www.academia.edu/Documents/in/Pharmacy">Pharmacy</a>, <script data-card-contents-for-ri="656" type="text/json">{"id":656,"name":"Pharmacy","url":"https://www.academia.edu/Documents/in/Pharmacy?f_ri=4656","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="2215" href="https://www.academia.edu/Documents/in/Water">Water</a>, <script data-card-contents-for-ri="2215" type="text/json">{"id":2215,"name":"Water","url":"https://www.academia.edu/Documents/in/Water?f_ri=4656","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="4523" href="https://www.academia.edu/Documents/in/Water_quality">Water quality</a><script data-card-contents-for-ri="4523" type="text/json">{"id":4523,"name":"Water quality","url":"https://www.academia.edu/Documents/in/Water_quality?f_ri=4656","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=28244455]'), work: {"id":28244455,"title":"Removal of Endotoxin from Protein in Pharmaceutical Processes","created_at":"2016-09-04T09:41:45.846-07:00","url":"https://www.academia.edu/28244455/Removal_of_Endotoxin_from_Protein_in_Pharmaceutical_Processes?f_ri=4656","dom_id":"work_28244455","summary":"Bacterial endotoxin is the lipopolysaccharide component of the cell wall of Gram-negative bacteria, together with other cellular material that combines to form an endotoxin complex. Endotoxin is pyrogenic and it presents a risk to patients who are administered intravenous and intramuscular preparations.1Thus bacterial endotoxins pose a risk to many pharmaceutical processes and, where not controlled, to the finished products. There are different methods for endotoxin removal. These include depyrogenation,2 such as dry-heat processes applied to glassware, and rinsing,3 as might be applied to closures. These areas receive reasonable coverage within the pharmaceutical sector. Areas that are actively discussed within biotechnology fields but which receive less attention in the wider pharmaceutical context are steps to remove endotoxin bound to protein. This article considers some of the biotechnological applications for endotoxin removal.","downloadable_attachments":[{"id":48564229,"asset_id":28244455,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":725124,"first_name":"Tim","last_name":"Sandle","domain_name":"manchester","page_name":"TimSandle","display_name":"Tim Sandle","profile_url":"https://manchester.academia.edu/TimSandle?f_ri=4656","photo":"https://0.academia-photos.com/725124/247895/293007/s65_tim.sandle.jpg"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology?f_ri=4656","nofollow":false},{"id":656,"name":"Pharmacy","url":"https://www.academia.edu/Documents/in/Pharmacy?f_ri=4656","nofollow":false},{"id":2215,"name":"Water","url":"https://www.academia.edu/Documents/in/Water?f_ri=4656","nofollow":false},{"id":4523,"name":"Water 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href="https://www.academia.edu/Documents/in/Pharmaceutical_Technology">Pharmaceutical Technology</a><script data-card-contents-for-ri="9186" type="text/json">{"id":9186,"name":"Pharmaceutical Technology","url":"https://www.academia.edu/Documents/in/Pharmaceutical_Technology?f_ri=4656","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=12461636]'), work: {"id":12461636,"title":"Sample preparation for chromatography book","created_at":"2015-05-19T04:28:49.279-07:00","url":"https://www.academia.edu/12461636/Sample_preparation_for_chromatography_book?f_ri=4656","dom_id":"work_12461636","summary":null,"downloadable_attachments":[{"id":37677681,"asset_id":12461636,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":4522162,"first_name":"Dini","last_name":"Nurhaliza","domain_name":"cdutcm","page_name":"DiniNurhaliza","display_name":"Dini 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href="https://www.academia.edu/25651827/Analysis_of_Agarwood_oil_Aquilaria_Malaccensis_based_on_GC_MS_data">Analysis of Agarwood oil (Aquilaria Malaccensis) based on GC-MS data</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/25651827" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="684e0eba454d5dfed426aaa4a85a54f4" rel="nofollow" data-download="{"attachment_id":45989472,"asset_id":25651827,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm 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data-has-card-for-ri="4656" href="https://www.academia.edu/Documents/in/Chromatography">Chromatography</a>, <script data-card-contents-for-ri="4656" type="text/json">{"id":4656,"name":"Chromatography","url":"https://www.academia.edu/Documents/in/Chromatography?f_ri=4656","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="5069" href="https://www.academia.edu/Documents/in/Principal_Component_Analysis">Principal Component Analysis</a><script data-card-contents-for-ri="5069" type="text/json">{"id":5069,"name":"Principal Component Analysis","url":"https://www.academia.edu/Documents/in/Principal_Component_Analysis?f_ri=4656","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=25651827]'), work: {"id":25651827,"title":"Analysis of Agarwood oil (Aquilaria Malaccensis) based on GC-MS 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Medicine","url":"https://www.academia.edu/Documents/in/Traditional_Medicine?f_ri=4656","nofollow":false},{"id":4656,"name":"Chromatography","url":"https://www.academia.edu/Documents/in/Chromatography?f_ri=4656","nofollow":false},{"id":5069,"name":"Principal Component Analysis","url":"https://www.academia.edu/Documents/in/Principal_Component_Analysis?f_ri=4656","nofollow":false},{"id":5187,"name":"Statistical Analysis","url":"https://www.academia.edu/Documents/in/Statistical_Analysis?f_ri=4656"},{"id":43520,"name":"Middle East","url":"https://www.academia.edu/Documents/in/Middle_East?f_ri=4656"},{"id":54123,"name":"Artificial Neural 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St. Hil. (Mate) Unripe Fruits</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/79076418" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="f7cebcfe9a0c77bc03bca74a7906bd26" rel="nofollow" data-download="{"attachment_id":85919319,"asset_id":79076418,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/85919319/download_file?st=MTczMjQwNDEzOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="56004126" href="https://independent.academia.edu/SVerza">Simone Verza</a><script data-card-contents-for-user="56004126" type="text/json">{"id":56004126,"first_name":"Simone","last_name":"Verza","domain_name":"independent","page_name":"SVerza","display_name":"Simone Verza","profile_url":"https://independent.academia.edu/SVerza?f_ri=4656","photo":"https://0.academia-photos.com/56004126/23384230/22456546/s65_simone.verza.jpg"}</script></span></span></li><li class="js-paper-rank-work_79076418 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="79076418"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 79076418, container: ".js-paper-rank-work_79076418", }); 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$(".js-view-count[data-work-id=79076418]").text(description); $(".js-view-count-work_79076418").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_79076418").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="79076418"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">20</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="523" href="https://www.academia.edu/Documents/in/Chemistry">Chemistry</a>, <script data-card-contents-for-ri="523" type="text/json">{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry?f_ri=4656","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="4656" href="https://www.academia.edu/Documents/in/Chromatography">Chromatography</a>, <script data-card-contents-for-ri="4656" type="text/json">{"id":4656,"name":"Chromatography","url":"https://www.academia.edu/Documents/in/Chromatography?f_ri=4656","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="5769" href="https://www.academia.edu/Documents/in/Mass_Spectrometry">Mass Spectrometry</a>, <script data-card-contents-for-ri="5769" type="text/json">{"id":5769,"name":"Mass Spectrometry","url":"https://www.academia.edu/Documents/in/Mass_Spectrometry?f_ri=4656","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="26327" href="https://www.academia.edu/Documents/in/Medicine">Medicine</a><script data-card-contents-for-ri="26327" type="text/json">{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine?f_ri=4656","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=79076418]'), work: {"id":79076418,"title":"LC-UV Assay Method and UPLC/Q-TOF-MS Characterisation of Saponins from Ilex Paraguariensis A. St. Hil. (Mate) Unripe Fruits","created_at":"2022-05-13T13:04:20.055-07:00","url":"https://www.academia.edu/79076418/LC_UV_Assay_Method_and_UPLC_Q_TOF_MS_Characterisation_of_Saponins_from_Ilex_Paraguariensis_A_St_Hil_Mate_Unripe_Fruits?f_ri=4656","dom_id":"work_79076418","summary":null,"downloadable_attachments":[{"id":85919319,"asset_id":79076418,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":56004126,"first_name":"Simone","last_name":"Verza","domain_name":"independent","page_name":"SVerza","display_name":"Simone Verza","profile_url":"https://independent.academia.edu/SVerza?f_ri=4656","photo":"https://0.academia-photos.com/56004126/23384230/22456546/s65_simone.verza.jpg"}],"research_interests":[{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry?f_ri=4656","nofollow":false},{"id":4656,"name":"Chromatography","url":"https://www.academia.edu/Documents/in/Chromatography?f_ri=4656","nofollow":false},{"id":5769,"name":"Mass 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SCIENCES","url":"https://www.academia.edu/Documents/in/CHEMICAL_SCIENCES?f_ri=4656"},{"id":297834,"name":"Ilex paraguariensis","url":"https://www.academia.edu/Documents/in/Ilex_paraguariensis?f_ri=4656"},{"id":354056,"name":"Plant extracts","url":"https://www.academia.edu/Documents/in/Plant_extracts?f_ri=4656"},{"id":383446,"name":"Phytochemical Analysis","url":"https://www.academia.edu/Documents/in/Phytochemical_Analysis?f_ri=4656"},{"id":1533653,"name":"Glycosides","url":"https://www.academia.edu/Documents/in/Glycosides?f_ri=4656"},{"id":1627795,"name":"Oleanolic acid","url":"https://www.academia.edu/Documents/in/Oleanolic_acid?f_ri=4656"},{"id":1724844,"name":"Molecular Structure","url":"https://www.academia.edu/Documents/in/Molecular_Structure?f_ri=4656"},{"id":2637271,"name":"Reference standards","url":"https://www.academia.edu/Documents/in/Reference_standards?f_ri=4656"},{"id":3763225,"name":"Medical and Health Sciences","url":"https://www.academia.edu/Documents/in/Medical_and_Health_Sciences?f_ri=4656"},{"id":3988601,"name":"limit of detection","url":"https://www.academia.edu/Documents/in/limit_of_detection?f_ri=4656"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_78442859" data-work_id="78442859" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/78442859/HPLC_Analysis_of_Monomer_Release_from_Conventionally_and_High_Temperature_High_Pressure_Polymerised_Urethane_Dimethacrylate_Intended_for_Biomedical_Applications">HPLC Analysis of Monomer Release from Conventionally and High Temperature High-Pressure Polymerised Urethane Dimethacrylate Intended for Biomedical Applications</a></div></div><div class="u-pb4x u-mt3x"></div><ul 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data-has-card-for-ri="523" href="https://www.academia.edu/Documents/in/Chemistry">Chemistry</a>, <script data-card-contents-for-ri="523" type="text/json">{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry?f_ri=4656","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="4656" href="https://www.academia.edu/Documents/in/Chromatography">Chromatography</a><script data-card-contents-for-ri="4656" type="text/json">{"id":4656,"name":"Chromatography","url":"https://www.academia.edu/Documents/in/Chromatography?f_ri=4656","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=78442859]'), work: {"id":78442859,"title":"HPLC Analysis of Monomer Release from Conventionally and High Temperature High-Pressure Polymerised Urethane Dimethacrylate Intended for Biomedical 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})();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_78262117" data-work_id="78262117" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/78262117/Rapid_in_process_monitoring_of_lentiviral_vector_particles_by_high_performance_liquid_chromatography">Rapid in-process monitoring of lentiviral vector particles by high performance liquid chromatography</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/78262117" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa 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itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="57732307" href="https://independent.academia.edu/AmineKamen">Amine Kamen</a><script data-card-contents-for-user="57732307" type="text/json">{"id":57732307,"first_name":"Amine","last_name":"Kamen","domain_name":"independent","page_name":"AmineKamen","display_name":"Amine Kamen","profile_url":"https://independent.academia.edu/AmineKamen?f_ri=4656","photo":"https://0.academia-photos.com/57732307/29554354/27493251/s65_amine.kamen.jpg"}</script></span></span></li><li class="js-paper-rank-work_78262117 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="78262117"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 78262117, container: ".js-paper-rank-work_78262117", }); });</script></li><li class="js-percentile-work_78262117 InlineList-item InlineList-item--bordered hidden u-tcGrayDark"><span class="percentile-widget hidden"><span class="u-mr2x percentile-widget" style="display: none">•</span><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 78262117; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-percentile-work_78262117"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_78262117 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="78262117"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 78262117; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=78262117]").text(description); $(".js-view-count-work_78262117").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_78262117").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="78262117"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i></div><span class="InlineList-item-text u-textTruncate u-pl6x"><a class="InlineList-item-text" data-has-card-for-ri="4656" href="https://www.academia.edu/Documents/in/Chromatography">Chromatography</a><script data-card-contents-for-ri="4656" type="text/json">{"id":4656,"name":"Chromatography","url":"https://www.academia.edu/Documents/in/Chromatography?f_ri=4656","nofollow":false}</script></span></li><script>(function(){ if (false) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=78262117]'), work: {"id":78262117,"title":"Rapid in-process monitoring of lentiviral vector particles by high performance liquid chromatography","created_at":"2022-05-02T14:28:03.346-07:00","url":"https://www.academia.edu/78262117/Rapid_in_process_monitoring_of_lentiviral_vector_particles_by_high_performance_liquid_chromatography?f_ri=4656","dom_id":"work_78262117","summary":null,"downloadable_attachments":[{"id":85370364,"asset_id":78262117,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":57732307,"first_name":"Amine","last_name":"Kamen","domain_name":"independent","page_name":"AmineKamen","display_name":"Amine Kamen","profile_url":"https://independent.academia.edu/AmineKamen?f_ri=4656","photo":"https://0.academia-photos.com/57732307/29554354/27493251/s65_amine.kamen.jpg"}],"research_interests":[{"id":4656,"name":"Chromatography","url":"https://www.academia.edu/Documents/in/Chromatography?f_ri=4656","nofollow":false}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_65332974" data-work_id="65332974" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/65332974/Physicochemical_Characteristics_of_Different_Pulp_and_Paper_Mill_Waste_Streams_for_Hydrothermal_Conversion">Physicochemical Characteristics of Different Pulp and Paper Mill Waste Streams for Hydrothermal Conversion</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The pulp and paper industry generates significant amounts of waste from various processes consisting of organic and inorganic matter. Due to its composition, the management of pulp and paper mill sludge presents an economic burden for... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_65332974" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The pulp and paper industry generates significant amounts of waste from various processes consisting of organic and inorganic matter. Due to its composition, the management of pulp and paper mill sludge presents an economic burden for wastewater treatment plants. In South Africa, paper mill sludge is mainly incinerated, landfilled or used for land application. These practices cause environmental and health hazards. In this study, a process of stabilization of paper mill sludge through hydrothermal carbonization is proposed. The quality of biochar produced and energy yield will depend on the elemental composition, calorific value and ash content of the sludge. Results revealed samples are composed of flat fibrous cellulosic morphology, with high volatile matter and little fixed carbon percentage. The main decomposition occurred between 220-400 °C. Sludge from primary clarifier contains the highest ash percentage, hence it was found to be unsuitable for energy recovery through hydroth...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/65332974" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="0f9d0fffb519d29de8fdea16cbb83422" rel="nofollow" data-download="{"attachment_id":76975724,"asset_id":65332974,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/76975724/download_file?st=MTczMjQwNDEzOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="75828745" href="https://up-za.academia.edu/EnglatinaAssis">Englatina Assis</a><script data-card-contents-for-user="75828745" type="text/json">{"id":75828745,"first_name":"Englatina","last_name":"Assis","domain_name":"up-za","page_name":"EnglatinaAssis","display_name":"Englatina Assis","profile_url":"https://up-za.academia.edu/EnglatinaAssis?f_ri=4656","photo":"https://0.academia-photos.com/75828745/55948715/44138697/s65_englatina.assis.jpeg"}</script></span></span></li><li class="js-paper-rank-work_65332974 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="65332974"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 65332974, container: ".js-paper-rank-work_65332974", }); 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Due to its composition, the management of pulp and paper mill sludge presents an economic burden for wastewater treatment plants. In South Africa, paper mill sludge is mainly incinerated, landfilled or used for land application. These practices cause environmental and health hazards. In this study, a process of stabilization of paper mill sludge through hydrothermal carbonization is proposed. The quality of biochar produced and energy yield will depend on the elemental composition, calorific value and ash content of the sludge. Results revealed samples are composed of flat fibrous cellulosic morphology, with high volatile matter and little fixed carbon percentage. The main decomposition occurred between 220-400 °C. Sludge from primary clarifier contains the highest ash percentage, hence it was found to be unsuitable for energy recovery through hydroth...","downloadable_attachments":[{"id":76975724,"asset_id":65332974,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":75828745,"first_name":"Englatina","last_name":"Assis","domain_name":"up-za","page_name":"EnglatinaAssis","display_name":"Englatina Assis","profile_url":"https://up-za.academia.edu/EnglatinaAssis?f_ri=4656","photo":"https://0.academia-photos.com/75828745/55948715/44138697/s65_englatina.assis.jpeg"}],"research_interests":[{"id":4656,"name":"Chromatography","url":"https://www.academia.edu/Documents/in/Chromatography?f_ri=4656","nofollow":false},{"id":72314,"name":"Fatty acids","url":"https://www.academia.edu/Documents/in/Fatty_acids?f_ri=4656","nofollow":false},{"id":109880,"name":"Candy","url":"https://www.academia.edu/Documents/in/Candy?f_ri=4656","nofollow":false},{"id":343667,"name":"Theoretical Models","url":"https://www.academia.edu/Documents/in/Theoretical_Models?f_ri=4656","nofollow":false},{"id":413194,"name":"Analysis of Variance","url":"https://www.academia.edu/Documents/in/Analysis_of_Variance?f_ri=4656"},{"id":1436039,"name":"Plant Oils","url":"https://www.academia.edu/Documents/in/Plant_Oils?f_ri=4656"},{"id":2348526,"name":"Dietary fats","url":"https://www.academia.edu/Documents/in/Dietary_fats?f_ri=4656"},{"id":3094724,"name":"Peroxides","url":"https://www.academia.edu/Documents/in/Peroxides?f_ri=4656"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_34643190" data-work_id="34643190" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/34643190/Separation_and_heat_stability_of_the_corticosteroid_induced_and_hepatic_alkaline_phosphatase_isoenzymes_in_canine_plasma">Separation and heat stability of the corticosteroid-induced and hepatic alkaline phosphatase isoenzymes in canine plasma</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/34643190" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="7edc49ddefe188147e28f37c164e4890" rel="nofollow" data-download="{"attachment_id":54503914,"asset_id":34643190,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/54503914/download_file?st=MTczMjQwNDEzOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="42150403" href="https://independent.academia.edu/ETeske">E. 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href="https://www.academia.edu/24590208/Analysis_of_steady_state_recycling_chromatography_using_equilibrium_theory">Analysis of steady state recycling chromatography using equilibrium theory</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/24590208" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="761c0963f3c1599a814cc4848cb7c872" rel="nofollow" data-download="{"attachment_id":44918939,"asset_id":24590208,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm 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Theory","url":"https://www.academia.edu/Documents/in/General_Equilibrium_Theory?f_ri=4656"},{"id":184965,"name":"Theoretical Analysis","url":"https://www.academia.edu/Documents/in/Theoretical_Analysis?f_ri=4656"},{"id":234860,"name":"Steady state","url":"https://www.academia.edu/Documents/in/Steady_state?f_ri=4656"},{"id":238198,"name":"Seismic analysis and design","url":"https://www.academia.edu/Documents/in/Seismic_analysis_and_design?f_ri=4656"},{"id":789521,"name":"Optimal Design","url":"https://www.academia.edu/Documents/in/Optimal_Design?f_ri=4656"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_15063137" data-work_id="15063137" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/15063137/A_new_sesquiterpene_lactone_from_Artemisia_rubripes_nakai">A new sesquiterpene lactone from Artemisia rubripes nakai</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The chromatographic separation of a methylene chloride extract of Artemisia rubripes led to the isolation of a new sesquiterpene lactone (3), together with four known compounds, a coumarin (2) and three terpenes (1, 4, and 5). Their... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_15063137" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The chromatographic separation of a methylene chloride extract of Artemisia rubripes led to the isolation of a new sesquiterpene lactone (3), together with four known compounds, a coumarin (2) and three terpenes (1, 4, and 5). Their structures were characterized to be 1beta,6alpha-dihydroxy-4(15)-eudesmene (1), scopoletin (2), 1alpha,4beta-dihydroxy-8alpha-acetoxy-guaia-2,10(14), 11(13)-triene-6,12-olide (3), 1alpha,4beta-dihydroxy-8alpha-acetoxy-guaia-2,9,11(13)-triene-6,12-olide (4), and beta-sitosterol-3-O-beta-D-glycoside (5) by spectroscopic means.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/15063137" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="f2c57768a4aa8b2f63e6971e6ceb4436" rel="nofollow" data-download="{"attachment_id":43608977,"asset_id":15063137,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/43608977/download_file?st=MTczMjQwNDEzOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="34089244" href="https://independent.academia.edu/kyulee3">kyu lee</a><script data-card-contents-for-user="34089244" type="text/json">{"id":34089244,"first_name":"kyu","last_name":"lee","domain_name":"independent","page_name":"kyulee3","display_name":"kyu lee","profile_url":"https://independent.academia.edu/kyulee3?f_ri=4656","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_15063137 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="15063137"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 15063137, container: ".js-paper-rank-work_15063137", }); 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Their structures were characterized to be 1beta,6alpha-dihydroxy-4(15)-eudesmene (1), scopoletin (2), 1alpha,4beta-dihydroxy-8alpha-acetoxy-guaia-2,10(14), 11(13)-triene-6,12-olide (3), 1alpha,4beta-dihydroxy-8alpha-acetoxy-guaia-2,9,11(13)-triene-6,12-olide (4), and beta-sitosterol-3-O-beta-D-glycoside (5) by spectroscopic means.","downloadable_attachments":[{"id":43608977,"asset_id":15063137,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":34089244,"first_name":"kyu","last_name":"lee","domain_name":"independent","page_name":"kyulee3","display_name":"kyu lee","profile_url":"https://independent.academia.edu/kyulee3?f_ri=4656","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":4656,"name":"Chromatography","url":"https://www.academia.edu/Documents/in/Chromatography?f_ri=4656","nofollow":false},{"id":5769,"name":"Mass 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u-tcGrayDarkest"><div class="summarized">We describe an isocratic, reversed-phase high-performance liquid chromatographic method for the simultaneous measurement of fully oxidised, dihydro- and tetrahydropterins in cerebrospinal fluid. Tetrahydrobiopterin is detected... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_62508742" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">We describe an isocratic, reversed-phase high-performance liquid chromatographic method for the simultaneous measurement of fully oxidised, dihydro- and tetrahydropterins in cerebrospinal fluid. 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u-tcGrayDarkest"><div class="summarized">Measurement of cardiac perfusion via agents such as 99mTc-sestamibi (Cardiolite; DuPont-Merck Pharmaceutical Co., Inc.) is widely used in clinical nuclear medicine for the diagnosis of coronary artery disease. The monograph for... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_47679187" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Measurement of cardiac perfusion via agents such as 99mTc-sestamibi (Cardiolite; DuPont-Merck Pharmaceutical Co., Inc.) is widely used in clinical nuclear medicine for the diagnosis of coronary artery disease. The monograph for 99mTc-sestamibi recommends at least 90% radiochemical purity (RCP) for clinical use. Various factors may influence the RCP of certain reagent kits. Some of these include the amount of activity added to the reagent kit, the generator ingrowth time, the generator manufacturer, the age of the eluate, and the age of the formulated kit. A D-optimal design with a 20-experiment run was devised to study the effects of these variables either alone or in combination on the RCP of 99mTc-sestamibi. The RCP was assessed by Baker-Flex thin-layer and high-performance liquid chromatographic methods, immediately and 6 h after reconstitution of the 99mTc-sestamibi. The results showed that 4 of the 5 variables studied were statistically significant predictors of the RCP. 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DuPont-Merck Pharmaceutical Co., Inc.) is widely used in clinical nuclear medicine for the diagnosis of coronary artery disease. The monograph for 99mTc-sestamibi recommends at least 90% radiochemical purity (RCP) for clinical use. Various factors may influence the RCP of certain reagent kits. Some of these include the amount of activity added to the reagent kit, the generator ingrowth time, the generator manufacturer, the age of the eluate, and the age of the formulated kit. A D-optimal design with a 20-experiment run was devised to study the effects of these variables either alone or in combination on the RCP of 99mTc-sestamibi. The RCP was assessed by Baker-Flex thin-layer and high-performance liquid chromatographic methods, immediately and 6 h after reconstitution of the 99mTc-sestamibi. The results showed that 4 of the 5 variables studied were statistically significant predictors of the RCP. The ag...","downloadable_attachments":[{"id":66646765,"asset_id":47679187,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":170617935,"first_name":"William","last_name":"Hladik","domain_name":"independent","page_name":"WilliamHladik","display_name":"William Hladik","profile_url":"https://independent.academia.edu/WilliamHladik?f_ri=4656","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":4656,"name":"Chromatography","url":"https://www.academia.edu/Documents/in/Chromatography?f_ri=4656","nofollow":false},{"id":37306,"name":"Quality Control","url":"https://www.academia.edu/Documents/in/Quality_Control?f_ri=4656","nofollow":false},{"id":44507,"name":"Nuclear","url":"https://www.academia.edu/Documents/in/Nuclear?f_ri=4656","nofollow":false},{"id":244814,"name":"Clinical Sciences","url":"https://www.academia.edu/Documents/in/Clinical_Sciences?f_ri=4656","nofollow":false},{"id":784086,"name":"Radiopharmaceuticals","url":"https://www.academia.edu/Documents/in/Radiopharmaceuticals?f_ri=4656"},{"id":3009044,"name":"quality assurance in health care","url":"https://www.academia.edu/Documents/in/quality_assurance_in_health_care?f_ri=4656"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_31246931" data-work_id="31246931" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/31246931/Adaptation_of_ICP_OES_routine_determination_techniques_for_the_analysis_of_rare_earth_elements_by_chromatographic_separation_in_geologic_materials_tests_with_reference_materials_and_granitic_rocks">Adaptation of ICP–OES routine determination techniques for the analysis of rare earth elements by chromatographic separation in geologic materials: tests with reference materials and granitic rocks</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">... Available online 12 March 2002. Abstract. The present contribution shows the results of tests on geological materials for determination of the rare-earth elements La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb, and Lu, as well as... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_31246931" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">... Available online 12 March 2002. Abstract. The present contribution shows the results of tests on geological materials for determination of the rare-earth elements La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb, and Lu, as well as Y and Sc. ...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/31246931" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="0e56ace53745a78a6d5bfb3d2aa6e001" rel="nofollow" data-download="{"attachment_id":54476284,"asset_id":31246931,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/54476284/download_file?st=MTczMjQwNDEzOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="59193224" href="https://usp-br.academia.edu/SandraAndrade">Sandra Andrade</a><script data-card-contents-for-user="59193224" type="text/json">{"id":59193224,"first_name":"Sandra","last_name":"Andrade","domain_name":"usp-br","page_name":"SandraAndrade","display_name":"Sandra Andrade","profile_url":"https://usp-br.academia.edu/SandraAndrade?f_ri=4656","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_31246931 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="31246931"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 31246931, container: ".js-paper-rank-work_31246931", }); 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Chromatography","url":"https://www.academia.edu/Documents/in/High_Performance_Liquid_Chromatography?f_ri=4656","nofollow":false},{"id":2711428,"name":"Propylparaben","url":"https://www.academia.edu/Documents/in/Propylparaben?f_ri=4656"},{"id":3996043,"name":"Paraben","url":"https://www.academia.edu/Documents/in/Paraben?f_ri=4656"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_80299964" data-work_id="80299964" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/80299964/Determination_of_Ketotifen_Fumarate_in_Syrup_Dosage_Form_by_High_Performance_Liquid_Chromatography">Determination of Ketotifen Fumarate in Syrup Dosage Form by High Performance Liquid Chromatography</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The goal of the current study was to establish and authenticate an isocratic reverse-stage High-Performance Liquid Chromatography (HPLC) method for quantifying ketotifen fumarate (KF) in pharmaceutical liquid dosage compositions. Easy,... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_80299964" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The goal of the current study was to establish and authenticate an isocratic reverse-stage High-Performance Liquid Chromatography (HPLC) method for quantifying ketotifen fumarate (KF) in pharmaceutical liquid dosage compositions. Easy, quick, accurate, exact, and accurate reverse-stage high-performance liquid chromatography was advanced for the simultaneous assessment of ketotifen fumarate in the liquid syrup dosage type. The HPLC system using isocratic elution method with reverse-phase Inertsil ODS-(250 mm × 4.6 mm, 3 μm) column was detected by ultraviolet absorbance at 297 nm with no interference from widely using excipients, the mobile phase (A) is a mixture of triethylamine and water (175 μl in 500 ml of water), and the mobile phase (B) is a mixture of triethylamine and methanol (175 μl in 500 ml of methanol) at a flow rate of 1.5 mL/min (mobile phase A 40 %:mobile phase B 60%) at column temperature using 40 ° C, the retention time for ketotifen fumarate was 6.4±0.5 min. The con...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/80299964" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="b0e8cf5dea92c77cf9dfce95a7627e4a" rel="nofollow" data-download="{"attachment_id":86727891,"asset_id":80299964,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/86727891/download_file?st=MTczMjQwNDEzOSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="95002081" href="https://independent.academia.edu/salarali5">salar ali</a><script data-card-contents-for-user="95002081" type="text/json">{"id":95002081,"first_name":"salar","last_name":"ali","domain_name":"independent","page_name":"salarali5","display_name":"salar ali","profile_url":"https://independent.academia.edu/salarali5?f_ri=4656","photo":"https://0.academia-photos.com/95002081/138218669/127686009/s65_salar.ali.jpeg"}</script></span></span></li><li class="js-paper-rank-work_80299964 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="80299964"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 80299964, container: ".js-paper-rank-work_80299964", }); 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Easy, quick, accurate, exact, and accurate reverse-stage high-performance liquid chromatography was advanced for the simultaneous assessment of ketotifen fumarate in the liquid syrup dosage type. The HPLC system using isocratic elution method with reverse-phase Inertsil ODS-(250 mm × 4.6 mm, 3 μm) column was detected by ultraviolet absorbance at 297 nm with no interference from widely using excipients, the mobile phase (A) is a mixture of triethylamine and water (175 μl in 500 ml of water), and the mobile phase (B) is a mixture of triethylamine and methanol (175 μl in 500 ml of methanol) at a flow rate of 1.5 mL/min (mobile phase A 40 %:mobile phase B 60%) at column temperature using 40 ° C, the retention time for ketotifen fumarate was 6.4±0.5 min. 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Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=4656"},{"id":53293,"name":"Software","url":"https://www.academia.edu/Documents/in/Software?f_ri=4656"},{"id":181569,"name":"Proteins","url":"https://www.academia.edu/Documents/in/Proteins?f_ri=4656"},{"id":291387,"name":"Mathematical Model","url":"https://www.academia.edu/Documents/in/Mathematical_Model?f_ri=4656"},{"id":343667,"name":"Theoretical Models","url":"https://www.academia.edu/Documents/in/Theoretical_Models?f_ri=4656"},{"id":1181939,"name":"PLANT PROTEINS","url":"https://www.academia.edu/Documents/in/PLANT_PROTEINS?f_ri=4656"},{"id":3508723,"name":"Ovalbumin ","url":"https://www.academia.edu/Documents/in/Ovalbumin?f_ri=4656"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_56424175" data-work_id="56424175" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/56424175/Solid_phase_extraction_and_determination_of_dansyl_derivatives_of_unconjugated_and_acetylated_polyamines_by_reversed_phase_liquid_chromatography_Improved_separation_systems_for_polyamines_in_cerebrospinal_fluid_urine_and_tissue">Solid-phase extraction and determination of dansyl derivatives of unconjugated and acetylated polyamines by reversed-phase liquid chromatography: Improved separation systems for polyamines in cerebrospinal fluid, urine and tissue</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/56424175" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa 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$('*[data-has-card-for-ri-list=56424175]'), work: {"id":56424175,"title":"Solid-phase extraction and determination of dansyl derivatives of unconjugated and acetylated polyamines by reversed-phase liquid chromatography: Improved separation systems for polyamines in cerebrospinal fluid, urine and tissue","created_at":"2021-10-07T20:05:16.259-07:00","url":"https://www.academia.edu/56424175/Solid_phase_extraction_and_determination_of_dansyl_derivatives_of_unconjugated_and_acetylated_polyamines_by_reversed_phase_liquid_chromatography_Improved_separation_systems_for_polyamines_in_cerebrospinal_fluid_urine_and_tissue?f_ri=4656","dom_id":"work_56424175","summary":null,"downloadable_attachments":[{"id":71818362,"asset_id":56424175,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":41483877,"first_name":"Laurence","last_name":"Marton","domain_name":"independent","page_name":"LaurenceMarton","display_name":"Laurence Marton","profile_url":"https://independent.academia.edu/LaurenceMarton?f_ri=4656","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":48,"name":"Engineering","url":"https://www.academia.edu/Documents/in/Engineering?f_ri=4656","nofollow":false},{"id":923,"name":"Technology","url":"https://www.academia.edu/Documents/in/Technology?f_ri=4656","nofollow":false},{"id":4656,"name":"Chromatography","url":"https://www.academia.edu/Documents/in/Chromatography?f_ri=4656","nofollow":false},{"id":46034,"name":"Polyamines","url":"https://www.academia.edu/Documents/in/Polyamines?f_ri=4656","nofollow":false},{"id":60971,"name":"Cerebrospinal Fluid","url":"https://www.academia.edu/Documents/in/Cerebrospinal_Fluid?f_ri=4656"},{"id":64568,"name":"Humans","url":"https://www.academia.edu/Documents/in/Humans?f_ri=4656"},{"id":83426,"name":"Solid Phase Extraction","url":"https://www.academia.edu/Documents/in/Solid_Phase_Extraction?f_ri=4656"},{"id":246560,"name":"High Pressure Liquid Chromatography","url":"https://www.academia.edu/Documents/in/High_Pressure_Liquid_Chromatography?f_ri=4656"},{"id":260118,"name":"CHEMICAL SCIENCES","url":"https://www.academia.edu/Documents/in/CHEMICAL_SCIENCES?f_ri=4656"},{"id":463081,"name":"Acetylation","url":"https://www.academia.edu/Documents/in/Acetylation?f_ri=4656"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_30074889" data-work_id="30074889" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/30074889/Staining_banded_human_chromosomes_with_Romanovsky_dyes_Some_practical_consequences_of_the_nature_of_the_stain">Staining banded human chromosomes with Romanovsky dyes: Some practical consequences of the nature of the stain</a></div></div><div class="u-pb4x 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href="https://www.academia.edu/Documents/in/Complementary_and_Alternative_Medicine">Complementary and Alternative Medicine</a><script data-card-contents-for-ri="4083" type="text/json">{"id":4083,"name":"Complementary and Alternative Medicine","url":"https://www.academia.edu/Documents/in/Complementary_and_Alternative_Medicine?f_ri=4656","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=30074889]'), work: {"id":30074889,"title":"Staining banded human chromosomes with Romanovsky dyes: Some practical consequences of the nature of the 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Genetics","url":"https://www.academia.edu/Documents/in/Medical_Genetics?f_ri=4656","nofollow":false},{"id":4083,"name":"Complementary and Alternative Medicine","url":"https://www.academia.edu/Documents/in/Complementary_and_Alternative_Medicine?f_ri=4656","nofollow":false},{"id":4656,"name":"Chromatography","url":"https://www.academia.edu/Documents/in/Chromatography?f_ri=4656"},{"id":54344,"name":"Methods","url":"https://www.academia.edu/Documents/in/Methods?f_ri=4656"},{"id":112334,"name":"Methanol","url":"https://www.academia.edu/Documents/in/Methanol?f_ri=4656"},{"id":196381,"name":"Methylene Blue","url":"https://www.academia.edu/Documents/in/Methylene_Blue?f_ri=4656"},{"id":396848,"name":"Formic Acid","url":"https://www.academia.edu/Documents/in/Formic_Acid?f_ri=4656"},{"id":413195,"name":"Time Factors","url":"https://www.academia.edu/Documents/in/Time_Factors?f_ri=4656"},{"id":1147230,"name":"Chromosomes","url":"https://www.academia.edu/Documents/in/Chromosomes?f_ri=4656"},{"id":1491514,"name":"*Hot Temperature","url":"https://www.academia.edu/Documents/in/_Hot_Temperature?f_ri=4656"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_26894983" data-work_id="26894983" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/26894983/Validation_of_a_new_procedure_to_determine_plasma_fatty_acid_concentration_and_isotopic_enrichment">Validation of a new procedure to determine plasma fatty acid concentration and isotopic enrichment</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Assessment of free fatty acid (FFA) concentration and isotopic enrichment is useful for studies of FFA kinetics in vivo. A new procedure to recover the major FFA from plasma for concentration and isotopic enrichment measurements is... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_26894983" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Assessment of free fatty acid (FFA) concentration and isotopic enrichment is useful for studies of FFA kinetics in vivo. A new procedure to recover the major FFA from plasma for concentration and isotopic enrichment measurements is described and validated. The procedure involves extraction of plasma lipids with hexane, methylation with iodomethane (CH(3)I) to form fatty acid methyl esters (FAME), and subsequent purification of FAME by solid phase extraction (SPE) chromatography. The new method was compared with a traditional method using thin-layer chromatography (TLC) to recover plasma FFA, with subsequent methylation by BF(3)/methanol. The TLC method was found to be less reliable than the new CH(3)I method because of contamination with extraneous fatty acids, chemical fractionation of FFA species, and incomplete recovery of FFA associated with TLC. In contrast, the CH(3)I/SPE method was free of contamination, did not exhibit chemical fractionation, and had higher recovery. The iod...</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/26894983" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="b695f35f32f743c82332fc8ac76c616e" rel="nofollow" data-download="{"attachment_id":47159066,"asset_id":26894983,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/47159066/download_file?st=MTczMjQwNDE0MCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="30570644" href="https://wustl.academia.edu/BrucePatterson">Bruce Patterson</a><script data-card-contents-for-user="30570644" type="text/json">{"id":30570644,"first_name":"Bruce","last_name":"Patterson","domain_name":"wustl","page_name":"BrucePatterson","display_name":"Bruce Patterson","profile_url":"https://wustl.academia.edu/BrucePatterson?f_ri=4656","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_26894983 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="26894983"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 26894983, container: ".js-paper-rank-work_26894983", }); 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$(".js-view-count[data-work-id=26894983]").text(description); $(".js-view-count-work_26894983").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_26894983").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="26894983"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">18</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="4656" href="https://www.academia.edu/Documents/in/Chromatography">Chromatography</a>, <script data-card-contents-for-ri="4656" type="text/json">{"id":4656,"name":"Chromatography","url":"https://www.academia.edu/Documents/in/Chromatography?f_ri=4656","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="4987" href="https://www.academia.edu/Documents/in/Kinetics">Kinetics</a>, <script data-card-contents-for-ri="4987" type="text/json">{"id":4987,"name":"Kinetics","url":"https://www.academia.edu/Documents/in/Kinetics?f_ri=4656","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="64568" href="https://www.academia.edu/Documents/in/Humans">Humans</a>, <script data-card-contents-for-ri="64568" type="text/json">{"id":64568,"name":"Humans","url":"https://www.academia.edu/Documents/in/Humans?f_ri=4656","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="72314" href="https://www.academia.edu/Documents/in/Fatty_acids">Fatty acids</a><script data-card-contents-for-ri="72314" type="text/json">{"id":72314,"name":"Fatty acids","url":"https://www.academia.edu/Documents/in/Fatty_acids?f_ri=4656","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=26894983]'), work: {"id":26894983,"title":"Validation of a new procedure to determine plasma fatty acid concentration and isotopic enrichment","created_at":"2016-07-11T06:07:19.271-07:00","url":"https://www.academia.edu/26894983/Validation_of_a_new_procedure_to_determine_plasma_fatty_acid_concentration_and_isotopic_enrichment?f_ri=4656","dom_id":"work_26894983","summary":"Assessment of free fatty acid (FFA) concentration and isotopic enrichment is useful for studies of FFA kinetics in vivo. A new procedure to recover the major FFA from plasma for concentration and isotopic enrichment measurements is described and validated. The procedure involves extraction of plasma lipids with hexane, methylation with iodomethane (CH(3)I) to form fatty acid methyl esters (FAME), and subsequent purification of FAME by solid phase extraction (SPE) chromatography. The new method was compared with a traditional method using thin-layer chromatography (TLC) to recover plasma FFA, with subsequent methylation by BF(3)/methanol. The TLC method was found to be less reliable than the new CH(3)I method because of contamination with extraneous fatty acids, chemical fractionation of FFA species, and incomplete recovery of FFA associated with TLC. In contrast, the CH(3)I/SPE method was free of contamination, did not exhibit chemical fractionation, and had higher recovery. The iod...","downloadable_attachments":[{"id":47159066,"asset_id":26894983,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":30570644,"first_name":"Bruce","last_name":"Patterson","domain_name":"wustl","page_name":"BrucePatterson","display_name":"Bruce Patterson","profile_url":"https://wustl.academia.edu/BrucePatterson?f_ri=4656","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":4656,"name":"Chromatography","url":"https://www.academia.edu/Documents/in/Chromatography?f_ri=4656","nofollow":false},{"id":4987,"name":"Kinetics","url":"https://www.academia.edu/Documents/in/Kinetics?f_ri=4656","nofollow":false},{"id":64568,"name":"Humans","url":"https://www.academia.edu/Documents/in/Humans?f_ri=4656","nofollow":false},{"id":72314,"name":"Fatty acids","url":"https://www.academia.edu/Documents/in/Fatty_acids?f_ri=4656","nofollow":false},{"id":83426,"name":"Solid Phase Extraction","url":"https://www.academia.edu/Documents/in/Solid_Phase_Extraction?f_ri=4656"},{"id":87651,"name":"Methylation","url":"https://www.academia.edu/Documents/in/Methylation?f_ri=4656"},{"id":143483,"name":"Deuterium","url":"https://www.academia.edu/Documents/in/Deuterium?f_ri=4656"},{"id":469822,"name":"Fatty Acid Methyl Ester","url":"https://www.academia.edu/Documents/in/Fatty_Acid_Methyl_Ester?f_ri=4656"},{"id":486713,"name":"Fatty Acid","url":"https://www.academia.edu/Documents/in/Fatty_Acid?f_ri=4656"},{"id":529560,"name":"Gas Chromatography/mass Spectrometry","url":"https://www.academia.edu/Documents/in/Gas_Chromatography_mass_Spectrometry?f_ri=4656"},{"id":549280,"name":"Reproducibility of Results","url":"https://www.academia.edu/Documents/in/Reproducibility_of_Results?f_ri=4656"},{"id":717115,"name":"Lipid","url":"https://www.academia.edu/Documents/in/Lipid?f_ri=4656"},{"id":818710,"name":"Free Fatty Acid","url":"https://www.academia.edu/Documents/in/Free_Fatty_Acid?f_ri=4656"},{"id":888739,"name":"Quntitative Thin Layer Chromatography","url":"https://www.academia.edu/Documents/in/Quntitative_Thin_Layer_Chromatography?f_ri=4656"},{"id":1175860,"name":"Thin Layer Chromatography","url":"https://www.academia.edu/Documents/in/Thin_Layer_Chromatography?f_ri=4656"},{"id":1341074,"name":"Isotope Labeling","url":"https://www.academia.edu/Documents/in/Isotope_Labeling?f_ri=4656"},{"id":1358142,"name":"Methyl Ester","url":"https://www.academia.edu/Documents/in/Methyl_Ester?f_ri=4656"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology?f_ri=4656"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_11263849" data-work_id="11263849" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/11263849/Characterisation_of_acid_soluble_collagen_from_skins_of_young_and_adult_Nile_perch_Lates_niloticus_">Characterisation of acid soluble collagen from skins of young and adult Nile perch ( Lates niloticus)</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Acid soluble collagen (ASC) was extracted from the skins of young and adult Nile perch (Lates niloticus) using 0.5 M acetic acid and precipitation with 0.9 M NaCl. The ASC yields, on a dry weight basis, were 63.1 and 58.7%, respectively... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_11263849" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Acid soluble collagen (ASC) was extracted from the skins of young and adult Nile perch (Lates niloticus) using 0.5 M acetic acid and precipitation with 0.9 M NaCl. The ASC yields, on a dry weight basis, were 63.1 and 58.7%, respectively for young and adult fish skins. SDS-PAGE showed that the collagens contained two alpha components (α1 and α2). ASC from Nile perch was found to contain more imino acids (19.3 and 20.0%, respectively, for young and adult fish) than most fish species. The denaturation temperature for the collagens from the skins of young and adult Nile perch was determined to be 36 °C, which is also higher than that for most other fish species. Fourier transform infrared spectroscopy showed a higher degree of molecular order in ASC from adult than from young Nile perch. The results indicate that age-related changes in Nile perch skin collagen are not very pronounced, probably because there is minimal development of mature cross-links.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/11263849" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="742999089f3f9b746a1b05e51af3cdae" rel="nofollow" data-download="{"attachment_id":46784344,"asset_id":11263849,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/46784344/download_file?st=MTczMjQwNDE0MCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="27202915" href="https://independent.academia.edu/JohnMuyonga">John Muyonga</a><script data-card-contents-for-user="27202915" type="text/json">{"id":27202915,"first_name":"John","last_name":"Muyonga","domain_name":"independent","page_name":"JohnMuyonga","display_name":"John Muyonga","profile_url":"https://independent.academia.edu/JohnMuyonga?f_ri=4656","photo":"https://0.academia-photos.com/27202915/7708807/8644828/s65_john.muyonga.jpg"}</script></span></span></li><li class="js-paper-rank-work_11263849 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="11263849"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 11263849, container: ".js-paper-rank-work_11263849", }); 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$(".js-view-count[data-work-id=11263849]").text(description); $(".js-view-count-work_11263849").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_11263849").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="11263849"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">14</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="4656" href="https://www.academia.edu/Documents/in/Chromatography">Chromatography</a>, <script data-card-contents-for-ri="4656" type="text/json">{"id":4656,"name":"Chromatography","url":"https://www.academia.edu/Documents/in/Chromatography?f_ri=4656","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="16137" href="https://www.academia.edu/Documents/in/Food_Chemistry">Food Chemistry</a>, <script data-card-contents-for-ri="16137" type="text/json">{"id":16137,"name":"Food Chemistry","url":"https://www.academia.edu/Documents/in/Food_Chemistry?f_ri=4656","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="28235" href="https://www.academia.edu/Documents/in/Multidisciplinary">Multidisciplinary</a>, <script data-card-contents-for-ri="28235" type="text/json">{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary?f_ri=4656","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="133177" href="https://www.academia.edu/Documents/in/Temperature">Temperature</a><script data-card-contents-for-ri="133177" type="text/json">{"id":133177,"name":"Temperature","url":"https://www.academia.edu/Documents/in/Temperature?f_ri=4656","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=11263849]'), work: {"id":11263849,"title":"Characterisation of acid soluble collagen from skins of young and adult Nile perch ( Lates niloticus)","created_at":"2015-03-04T07:30:55.075-08:00","url":"https://www.academia.edu/11263849/Characterisation_of_acid_soluble_collagen_from_skins_of_young_and_adult_Nile_perch_Lates_niloticus_?f_ri=4656","dom_id":"work_11263849","summary":"Acid soluble collagen (ASC) was extracted from the skins of young and adult Nile perch (Lates niloticus) using 0.5 M acetic acid and precipitation with 0.9 M NaCl. The ASC yields, on a dry weight basis, were 63.1 and 58.7%, respectively for young and adult fish skins. SDS-PAGE showed that the collagens contained two alpha components (α1 and α2). ASC from Nile perch was found to contain more imino acids (19.3 and 20.0%, respectively, for young and adult fish) than most fish species. The denaturation temperature for the collagens from the skins of young and adult Nile perch was determined to be 36 °C, which is also higher than that for most other fish species. Fourier transform infrared spectroscopy showed a higher degree of molecular order in ASC from adult than from young Nile perch. The results indicate that age-related changes in Nile perch skin collagen are not very pronounced, probably because there is minimal development of mature cross-links.","downloadable_attachments":[{"id":46784344,"asset_id":11263849,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":27202915,"first_name":"John","last_name":"Muyonga","domain_name":"independent","page_name":"JohnMuyonga","display_name":"John Muyonga","profile_url":"https://independent.academia.edu/JohnMuyonga?f_ri=4656","photo":"https://0.academia-photos.com/27202915/7708807/8644828/s65_john.muyonga.jpg"}],"research_interests":[{"id":4656,"name":"Chromatography","url":"https://www.academia.edu/Documents/in/Chromatography?f_ri=4656","nofollow":false},{"id":16137,"name":"Food Chemistry","url":"https://www.academia.edu/Documents/in/Food_Chemistry?f_ri=4656","nofollow":false},{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary?f_ri=4656","nofollow":false},{"id":133177,"name":"Temperature","url":"https://www.academia.edu/Documents/in/Temperature?f_ri=4656","nofollow":false},{"id":162553,"name":"Skin","url":"https://www.academia.edu/Documents/in/Skin?f_ri=4656"},{"id":372258,"name":"Carp","url":"https://www.academia.edu/Documents/in/Carp?f_ri=4656"},{"id":398650,"name":"Fourier transform infrared spectroscopy","url":"https://www.academia.edu/Documents/in/Fourier_transform_infrared_spectroscopy?f_ri=4656"},{"id":447999,"name":"Denaturation","url":"https://www.academia.edu/Documents/in/Denaturation?f_ri=4656"},{"id":494836,"name":"ASC","url":"https://www.academia.edu/Documents/in/ASC?f_ri=4656"},{"id":613425,"name":"Nile Perch","url":"https://www.academia.edu/Documents/in/Nile_Perch?f_ri=4656"},{"id":723149,"name":"Acetic Acid","url":"https://www.academia.edu/Documents/in/Acetic_Acid?f_ri=4656"},{"id":1274450,"name":"Conformational Change","url":"https://www.academia.edu/Documents/in/Conformational_Change?f_ri=4656"},{"id":2281767,"name":"Type I Collagen","url":"https://www.academia.edu/Documents/in/Type_I_Collagen?f_ri=4656"},{"id":2471742,"name":"Dry Weight","url":"https://www.academia.edu/Documents/in/Dry_Weight?f_ri=4656"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_12508171" data-work_id="12508171" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/12508171/Functionalized_SBA_15_mesoporous_silica_in_ion_chromatography_of_alkali_alkaline_earths_ammonium_and_transition_metal_ions">Functionalized SBA-15 mesoporous silica in ion chromatography of alkali, alkaline earths, ammonium and transition metal ions</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/12508171" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="aea94545cfff9e2dc11d4f84d2effc88" rel="nofollow" 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class="summarized">Molasses desugarization (MDS) is an example of a large-scale chromatographic separation that has been in use in the United States beet sugar industry for almost 40 years. The process has proven commercially viable under current market... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_40367371" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Molasses desugarization (MDS) is an example of a large-scale chromatographic separation that has been in use in the United States beet sugar industry for almost 40 years. The process has proven commercially viable under current market conditions, energy and environmental cost. As a result, most US sugar beet companies have been operating one or more MDS installations. Because of the variation in efficiencies and configuration of existing installations, it is sometimes difficult to evaluate the desired separation targets and identify the areas of operational improvement. Over the last few years, achievable separation targets have improved significantly, and new equipment configurations have been developed. Initially, a calculated sugar recovery (“sugar in the bag vs sugar in molasses”) of 75-78 % was considered acceptable. In modern systems, up to 88 % efficiency is commercially achievable. However, almost no guidance is available in the recent literature about how to achieve these results for various process configurations. The current presentation will attempt to provide these answers as well as assist in a general understanding of large-scale chromatographic technologies, existing types of equipment and factors that influence separation efficiencies. For many years, MDS systems were installed as stand-alone operations that could easily be “plugged” into conventional sugar factories. As chromatographic extract has notably different characteristics compared to thick juice, special consideration will be given to the way the extract processing is integrated in factories.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/40367371" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="cf90d5487b1c1e23f5497729b883766d" rel="nofollow" data-download="{"attachment_id":60614792,"asset_id":40367371,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/60614792/download_file?st=MTczMjQwNDE0MCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="58186654" href="https://independentresearcher.academia.edu/StevePeacock">Steve Peacock</a><script data-card-contents-for-user="58186654" type="text/json">{"id":58186654,"first_name":"Steve","last_name":"Peacock","domain_name":"independentresearcher","page_name":"StevePeacock","display_name":"Steve Peacock","profile_url":"https://independentresearcher.academia.edu/StevePeacock?f_ri=4656","photo":"https://0.academia-photos.com/58186654/15240402/18180360/s65_steve.peacock.jpg"}</script></span></span></li><li class="js-paper-rank-work_40367371 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="40367371"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 40367371, container: ".js-paper-rank-work_40367371", }); 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The process has proven commercially viable under current market conditions, energy and environmental cost. As a result, most US sugar beet companies have been operating one or more MDS installations. Because of the variation in efficiencies and configuration of existing installations, it is sometimes difficult to evaluate the desired separation targets and identify the areas of operational improvement. Over the last few years, achievable separation targets have improved significantly, and new equipment configurations have been developed. Initially, a calculated sugar recovery (“sugar in the bag vs sugar in molasses”) of 75-78 % was considered acceptable. In modern systems, up to 88 % efficiency is commercially achievable. However, almost no guidance is available in the recent literature about how to achieve these results for various process configurations. The current presentation will attempt to provide these answers as well as assist in a general understanding of large-scale chromatographic technologies, existing types of equipment and factors that influence separation efficiencies. For many years, MDS systems were installed as stand-alone operations that could easily be “plugged” into conventional sugar factories. 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Schneider , Licarion Pinto , Mario Cesar Ugulino de Araujo , Adriano de Ará ujo Gomes , Chromatographic quantification of seven pesticide residues in vegetable: univariate... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_40712395" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Please cite this article as: Emanuella Santos Sousa , Mateus P. 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In this chapter, megaporous cryogels were synthesized having metal-ion affinity functionality, and... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_34948385" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Immobilized metal-ion affinity chromatography (IMAC) has been developed for the rapid isolation and purification of recombinant proteins. In this chapter, megaporous cryogels were synthesized having metal-ion affinity functionality, and their adsorptive properties were investigated. These cryogels have large pore sizes ranging from 10 to 100 μm with corresponding porosities between 80 and 90 %. The synthesized IMAC-cryogel had a total ligand density of 770 μmol/g. Twelve milligram of a His 6-tagged protein (NAD (P)H-dependent 2-cyclohexen-1-one-reductase) can be purified from a crude cell extract per gram of IMAC-cryogels. The protein binding capacity is increased with higher degrees of grafting, although a slight decrease in column efficiency may result. This chapter provides methodologies for a rapid single-step purification of recombinant His 6-tagged proteins from crude cell extracts using IMAC-cryogels.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/34948385" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="7eb1d660dec43210c141536f1141542c" rel="nofollow" data-download="{"attachment_id":54810636,"asset_id":34948385,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/54810636/download_file?st=MTczMjQwNDE0MCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="623380" href="https://nd.academia.edu/Naveenkumarsingh">Naveen Kumar Singh</a><script data-card-contents-for-user="623380" type="text/json">{"id":623380,"first_name":"Naveen Kumar","last_name":"Singh","domain_name":"nd","page_name":"Naveenkumarsingh","display_name":"Naveen Kumar Singh","profile_url":"https://nd.academia.edu/Naveenkumarsingh?f_ri=4656","photo":"https://0.academia-photos.com/623380/215912/18101865/s65_naveen_kumar.singh.png"}</script></span></span></li><li class="js-paper-rank-work_34948385 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="34948385"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 34948385, container: ".js-paper-rank-work_34948385", }); 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This chapter provides methodologies for a rapid single-step purification of recombinant His 6-tagged proteins from crude cell extracts using IMAC-cryogels.","downloadable_attachments":[{"id":54810636,"asset_id":34948385,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":623380,"first_name":"Naveen Kumar","last_name":"Singh","domain_name":"nd","page_name":"Naveenkumarsingh","display_name":"Naveen Kumar Singh","profile_url":"https://nd.academia.edu/Naveenkumarsingh?f_ri=4656","photo":"https://0.academia-photos.com/623380/215912/18101865/s65_naveen_kumar.singh.png"}],"research_interests":[{"id":3306,"name":"Protein Purification","url":"https://www.academia.edu/Documents/in/Protein_Purification?f_ri=4656","nofollow":false},{"id":4656,"name":"Chromatography","url":"https://www.academia.edu/Documents/in/Chromatography?f_ri=4656","nofollow":false},{"id":201241,"name":"Affinity 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type="text/json">{"id":9786,"name":"Proteomics","url":"https://www.academia.edu/Documents/in/Proteomics?f_ri=4656","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="47884" href="https://www.academia.edu/Documents/in/Biological_Sciences">Biological Sciences</a>, <script data-card-contents-for-ri="47884" type="text/json">{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences?f_ri=4656","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="57556" href="https://www.academia.edu/Documents/in/Hippocampus">Hippocampus</a><script data-card-contents-for-ri="57556" type="text/json">{"id":57556,"name":"Hippocampus","url":"https://www.academia.edu/Documents/in/Hippocampus?f_ri=4656","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=14644192]'), work: {"id":14644192,"title":"Integrated Proteomic 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data-work_id="19143216" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/19143216/Micellar_Liquid_Chromatography_Recent_Advances_and_Applications">Micellar Liquid Chromatography: Recent Advances and Applications</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/19143216" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="740142a8c886d8bcb6aab364dcf7fc12" rel="nofollow" data-download="{"attachment_id":40455265,"asset_id":19143216,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/40455265/download_file?st=MTczMjQwNDE0MCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="33031516" href="https://universite-lyon.academia.edu/AlainBerthod">Alain Berthod</a><script data-card-contents-for-user="33031516" 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itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/9041448/Identification_of_volatile_organic_compounds_VOCs_in_plastic_products_using_gas_chromatography_and_mass_spectrometry_GC_MS_">Identification of volatile organic compounds (VOCs) in plastic products using gas chromatography and mass spectrometry (GC/MS)</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Plastic materials are widely used in daily life. They contain a wide range of compounds with low molecular mass, including monomeric and oligomeric residues of polymerization, solvent-related chemicals residues, and various additives.... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_9041448" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Plastic materials are widely used in daily life. They contain a wide range of compounds with low molecular mass, including monomeric and oligomeric residues of polymerization, <br />solvent-related chemicals residues, and various additives. Plastic products made of expanded polystyrene (EPS) are currently employed as food containers. This study therefore sought to identify volatile organic compounds released by EPS from food packages and utensils used in Cartagena, Colombia. EPS-based plates, food and soup containers were subjected to various temperatures and released chemicals captured by solid phase microextraction, followed by on-column thermal desorption and gas chromatography/mass spectrometry analysis.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/9041448" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="257c78003d58aa666f1d2fbd8dfb5e4b" rel="nofollow" data-download="{"attachment_id":35346981,"asset_id":9041448,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/35346981/download_file?st=MTczMjQwNDE0MCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="9227947" href="https://independent.academia.edu/RevistaAmbiente%C3%81gua">Revista Ambiente & Água</a><script data-card-contents-for-user="9227947" type="text/json">{"id":9227947,"first_name":"Revista Ambiente","last_name":"\u0026 Água","domain_name":"independent","page_name":"RevistaAmbienteÁgua","display_name":"Revista Ambiente \u0026 Água","profile_url":"https://independent.academia.edu/RevistaAmbiente%C3%81gua?f_ri=4656","photo":"https://0.academia-photos.com/9227947/3021394/5212208/s65_revista_ambiente._gua.jpg"}</script></span></span></li><li class="js-paper-rank-work_9041448 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="9041448"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 9041448, container: ".js-paper-rank-work_9041448", }); 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They contain a wide range of compounds with low molecular mass, including monomeric and oligomeric residues of polymerization, \nsolvent-related chemicals residues, and various additives. Plastic products made of expanded polystyrene (EPS) are currently employed as food containers. This study therefore sought to identify volatile organic compounds released by EPS from food packages and utensils used in Cartagena, Colombia. EPS-based plates, food and soup containers were subjected to various temperatures and released chemicals captured by solid phase microextraction, followed by on-column thermal desorption and gas chromatography/mass spectrometry analysis. ","downloadable_attachments":[{"id":35346981,"asset_id":9041448,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":9227947,"first_name":"Revista Ambiente","last_name":"\u0026 Água","domain_name":"independent","page_name":"RevistaAmbienteÁgua","display_name":"Revista Ambiente \u0026 Água","profile_url":"https://independent.academia.edu/RevistaAmbiente%C3%81gua?f_ri=4656","photo":"https://0.academia-photos.com/9227947/3021394/5212208/s65_revista_ambiente._gua.jpg"}],"research_interests":[{"id":4656,"name":"Chromatography","url":"https://www.academia.edu/Documents/in/Chromatography?f_ri=4656","nofollow":false},{"id":5769,"name":"Mass Spectrometry","url":"https://www.academia.edu/Documents/in/Mass_Spectrometry?f_ri=4656","nofollow":false},{"id":161542,"name":"Solid Phase Microextraction","url":"https://www.academia.edu/Documents/in/Solid_Phase_Microextraction?f_ri=4656","nofollow":false}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_65953576" data-work_id="65953576" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/65953576/Development_of_LC_method_for_the_simultaneous_determination_of_antidepressant_drug_combination_melitracen_hydrochloride_and_flupentixol_dihydrochloride_in_">Development of LC method for the simultaneous determination of antidepressant drug combination melitracen hydrochloride and flupentixol dihydrochloride in …</a></div></div><div class="u-pb4x u-mt3x"></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/65953576" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="8db637ff1df5c3e124b75fcda1491560" rel="nofollow" data-download="{"attachment_id":77330296,"asset_id":65953576,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/77330296/download_file?st=MTczMjQwNDE0MCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="67205780" href="https://independent.academia.edu/Dimalshah">Dimal shah</a><script data-card-contents-for-user="67205780" type="text/json">{"id":67205780,"first_name":"Dimal","last_name":"shah","domain_name":"independent","page_name":"Dimalshah","display_name":"Dimal shah","profile_url":"https://independent.academia.edu/Dimalshah?f_ri=4656","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_65953576 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="65953576"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 65953576, container: ".js-paper-rank-work_65953576", }); 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$(".js-view-count[data-work-id=65953576]").text(description); $(".js-view-count-work_65953576").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_65953576").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="65953576"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">2</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="523" href="https://www.academia.edu/Documents/in/Chemistry">Chemistry</a>, <script data-card-contents-for-ri="523" type="text/json">{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry?f_ri=4656","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="4656" href="https://www.academia.edu/Documents/in/Chromatography">Chromatography</a><script data-card-contents-for-ri="4656" type="text/json">{"id":4656,"name":"Chromatography","url":"https://www.academia.edu/Documents/in/Chromatography?f_ri=4656","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=65953576]'), work: {"id":65953576,"title":"Development of LC method for the simultaneous determination of antidepressant drug combination melitracen hydrochloride and flupentixol dihydrochloride in …","created_at":"2021-12-25T09:55:36.987-08:00","url":"https://www.academia.edu/65953576/Development_of_LC_method_for_the_simultaneous_determination_of_antidepressant_drug_combination_melitracen_hydrochloride_and_flupentixol_dihydrochloride_in_?f_ri=4656","dom_id":"work_65953576","summary":null,"downloadable_attachments":[{"id":77330296,"asset_id":65953576,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":67205780,"first_name":"Dimal","last_name":"shah","domain_name":"independent","page_name":"Dimalshah","display_name":"Dimal shah","profile_url":"https://independent.academia.edu/Dimalshah?f_ri=4656","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":523,"name":"Chemistry","url":"https://www.academia.edu/Documents/in/Chemistry?f_ri=4656","nofollow":false},{"id":4656,"name":"Chromatography","url":"https://www.academia.edu/Documents/in/Chromatography?f_ri=4656","nofollow":false}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_35798465" data-work_id="35798465" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/35798465/On_the_relationship_between_radial_structure_heterogeneities_and_efficiency_of_chromatographic_columns">On the relationship between radial structure heterogeneities and efficiency of chromatographic columns</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Transverse structural column heterogeneities Structure–performance relationship Aris general dispersion theory Asymptotic and pre-asymptotic dispersion regimes Sub-2 m particles a b s t r a c t The general dispersion theory of Aris is... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_35798465" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Transverse structural column heterogeneities Structure–performance relationship Aris general dispersion theory Asymptotic and pre-asymptotic dispersion regimes Sub-2 m particles a b s t r a c t The general dispersion theory of Aris is applied to predict the virtual asymptotic dispersion behavior of packed columns. The derived model is also used to estimate the actual pre-asymptotic dispersion behavior of modern 2.1 mm × 50 mm columns packed with sub-2 m fully porous particles (FPPs) during the transient dispersion regime. The model accounts for the actual radial distribution of the flow velocity across the column diameter. From the wall to the center of the column, focused-ion-beam scanning electron microscopy (FIB-SEM) experiments were recently performed to reveal the existence of a thin (0.15d p wide, d p is the average particle diameter) hydrodynamic boundary layer (THBL), a thin (3d p wide) and loose orderly packed layer (TLOPL), a 60d p wide and dense randomly packed layer (WDRPL), and a large (460d p) randomly packed bulk central region [1]. The theoretical calculations of the actual pre-asymptotic reduced van Deemter curves (2.1 mm × 50 mm column, sub-2 m BEH-C 18 FPPs, n-hexanophenone analyte, acetonitrile/water eluent, 80/20, v/v, flow rate from 0.05 to 0.35 mL/min) confirm that the impact of the sole THBL on column dispersion can be neglected. In contrast, the contribution of the TLOPL to the reduced plate height (RPH) is about 0.2 h unit at optimum reduced velocity. Most remarkably, the negative impact of the TLOPL on column performance may be fully compensated by the presence of the adjacent WDRPL if the depth of the velocity well were to be 5% of the bulk velocity. In actual 2.1 mm × 50 mm columns packed with sub-2 m FPPs, this velocity depth is as large as 25% of the bulk velocity causing a significant RPH deviation of 0.7 h unit from the RPH of the bulk packing free from wall effects. Maximum column performance is expected for a reduction of WDRPL density. This suggests optimizing the packing process by finding the proper balance between the stress gradient across the WDRPL (responsible for the deep velocity well) and the friction forces between the packed particles (responsible for the rearrangement of the particles during bed consolidation). Past and recently reported RPH data support the theoretical insights: the stress gradient/particle friction balance in the WDRPL is better realized when packing superficially porous particles (SPPs) rather than FPPs in 2.1–4.6 mm i.d. columns (the RPH deviation is reduced to 0.4 h unit) or sub-2 m particles in 100 cm × 75 m i.d. capillaries combining high slurry concentrations and sonication (the RPH deviation is reduced to only 0.15 h unit).</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/35798465" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="578b1fc4ae946a80e81b1fe37cd8b398" rel="nofollow" data-download="{"attachment_id":55674950,"asset_id":35798465,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/55674950/download_file?st=MTczMjQwNDE0MCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="39835942" href="https://utk.academia.edu/FabriceGritti">Fabrice G E Gritti</a><script data-card-contents-for-user="39835942" type="text/json">{"id":39835942,"first_name":"Fabrice","last_name":"Gritti","domain_name":"utk","page_name":"FabriceGritti","display_name":"Fabrice G E Gritti","profile_url":"https://utk.academia.edu/FabriceGritti?f_ri=4656","photo":"https://0.academia-photos.com/39835942/10931478/52099074/s65_fabrice.gritti.jpg"}</script></span></span></li><li class="js-paper-rank-work_35798465 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="35798465"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 35798465, container: ".js-paper-rank-work_35798465", }); 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$(".js-view-count[data-work-id=35798465]").text(description); $(".js-view-count-work_35798465").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_35798465").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="35798465"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">5</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="4656" href="https://www.academia.edu/Documents/in/Chromatography">Chromatography</a>, <script data-card-contents-for-ri="4656" type="text/json">{"id":4656,"name":"Chromatography","url":"https://www.academia.edu/Documents/in/Chromatography?f_ri=4656","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="57608" href="https://www.academia.edu/Documents/in/Dispersion">Dispersion</a>, <script data-card-contents-for-ri="57608" type="text/json">{"id":57608,"name":"Dispersion","url":"https://www.academia.edu/Documents/in/Dispersion?f_ri=4656","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="225787" href="https://www.academia.edu/Documents/in/High_Performance_Liquid_Chromatography">High Performance Liquid Chromatography</a>, <script data-card-contents-for-ri="225787" type="text/json">{"id":225787,"name":"High Performance Liquid Chromatography","url":"https://www.academia.edu/Documents/in/High_Performance_Liquid_Chromatography?f_ri=4656","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="703396" href="https://www.academia.edu/Documents/in/Chromatographic_efficiency">Chromatographic efficiency</a><script data-card-contents-for-ri="703396" type="text/json">{"id":703396,"name":"Chromatographic efficiency","url":"https://www.academia.edu/Documents/in/Chromatographic_efficiency?f_ri=4656","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=35798465]'), work: {"id":35798465,"title":"On the relationship between radial structure heterogeneities and efficiency of chromatographic columns","created_at":"2018-01-30T14:10:17.917-08:00","url":"https://www.academia.edu/35798465/On_the_relationship_between_radial_structure_heterogeneities_and_efficiency_of_chromatographic_columns?f_ri=4656","dom_id":"work_35798465","summary":"Transverse structural column heterogeneities Structure–performance relationship Aris general dispersion theory Asymptotic and pre-asymptotic dispersion regimes Sub-2 m particles a b s t r a c t The general dispersion theory of Aris is applied to predict the virtual asymptotic dispersion behavior of packed columns. The derived model is also used to estimate the actual pre-asymptotic dispersion behavior of modern 2.1 mm × 50 mm columns packed with sub-2 m fully porous particles (FPPs) during the transient dispersion regime. The model accounts for the actual radial distribution of the flow velocity across the column diameter. From the wall to the center of the column, focused-ion-beam scanning electron microscopy (FIB-SEM) experiments were recently performed to reveal the existence of a thin (0.15d p wide, d p is the average particle diameter) hydrodynamic boundary layer (THBL), a thin (3d p wide) and loose orderly packed layer (TLOPL), a 60d p wide and dense randomly packed layer (WDRPL), and a large (460d p) randomly packed bulk central region [1]. The theoretical calculations of the actual pre-asymptotic reduced van Deemter curves (2.1 mm × 50 mm column, sub-2 m BEH-C 18 FPPs, n-hexanophenone analyte, acetonitrile/water eluent, 80/20, v/v, flow rate from 0.05 to 0.35 mL/min) confirm that the impact of the sole THBL on column dispersion can be neglected. In contrast, the contribution of the TLOPL to the reduced plate height (RPH) is about 0.2 h unit at optimum reduced velocity. Most remarkably, the negative impact of the TLOPL on column performance may be fully compensated by the presence of the adjacent WDRPL if the depth of the velocity well were to be 5% of the bulk velocity. In actual 2.1 mm × 50 mm columns packed with sub-2 m FPPs, this velocity depth is as large as 25% of the bulk velocity causing a significant RPH deviation of 0.7 h unit from the RPH of the bulk packing free from wall effects. Maximum column performance is expected for a reduction of WDRPL density. This suggests optimizing the packing process by finding the proper balance between the stress gradient across the WDRPL (responsible for the deep velocity well) and the friction forces between the packed particles (responsible for the rearrangement of the particles during bed consolidation). Past and recently reported RPH data support the theoretical insights: the stress gradient/particle friction balance in the WDRPL is better realized when packing superficially porous particles (SPPs) rather than FPPs in 2.1–4.6 mm i.d. columns (the RPH deviation is reduced to 0.4 h unit) or sub-2 m particles in 100 cm × 75 m i.d. capillaries combining high slurry concentrations and sonication (the RPH deviation is reduced to only 0.15 h unit).","downloadable_attachments":[{"id":55674950,"asset_id":35798465,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":39835942,"first_name":"Fabrice","last_name":"Gritti","domain_name":"utk","page_name":"FabriceGritti","display_name":"Fabrice G E Gritti","profile_url":"https://utk.academia.edu/FabriceGritti?f_ri=4656","photo":"https://0.academia-photos.com/39835942/10931478/52099074/s65_fabrice.gritti.jpg"}],"research_interests":[{"id":4656,"name":"Chromatography","url":"https://www.academia.edu/Documents/in/Chromatography?f_ri=4656","nofollow":false},{"id":57608,"name":"Dispersion","url":"https://www.academia.edu/Documents/in/Dispersion?f_ri=4656","nofollow":false},{"id":225787,"name":"High Performance Liquid Chromatography","url":"https://www.academia.edu/Documents/in/High_Performance_Liquid_Chromatography?f_ri=4656","nofollow":false},{"id":703396,"name":"Chromatographic efficiency","url":"https://www.academia.edu/Documents/in/Chromatographic_efficiency?f_ri=4656","nofollow":false},{"id":980014,"name":"Structure Property Correlations","url":"https://www.academia.edu/Documents/in/Structure_Property_Correlations?f_ri=4656"}]}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_2411560" data-work_id="2411560" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/2411560/Determination_of_pesticides_in_water_by_cone_shaped_membrane_protected_liquid_phase_microextraction_prior_to_micro_liquid_chromatography">Determination of pesticides in water by cone-shaped membrane protected liquid phase microextraction prior to micro-liquid chromatography</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">A new sample pre-treatment technique termed cone-shaped membrane liquid phase microextraction (CSM-LPME) was developed and combined with micro-liquid chromatography (micro-LC) for the determination of selected pesticides in water samples.... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_2411560" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">A new sample pre-treatment technique termed cone-shaped membrane liquid phase microextraction (CSM-LPME) was developed and combined with micro-liquid chromatography (micro-LC) for the determination of selected pesticides in water samples. Four pesticides (hexaconazole, procymidone, quinalphos and vinclozolin) were considered as target analytes. Several important extraction parameters such as types of extraction solvent, agitation rate, pH value, total exposure time and effect of salt and humic acids were optimized. Enrichment factors of >50 folds were easily achieved within 20 min of extraction. The analytical data demonstrated relative standard deviations for the reproducibility of the optimized CSM-LPME method ranging from 6.3 to 7.5%. The correlation coefficients of the calibration curves were at least 0.9995 across a concentration range of 2–100 μg/L. The detection limits for all the analytes were found to be in the range of 1.1–1.9 μg/L.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/2411560" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="54f004e9eeb82ed88100fa29c4840807" rel="nofollow" data-download="{"attachment_id":50639443,"asset_id":2411560,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/50639443/download_file?st=MTczMjQwNDE0MCw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="90616" href="https://teknologimalaysia.academia.edu/wanainiwanibrahim">WAN AINI WAN IBRAHIM</a><script data-card-contents-for-user="90616" type="text/json">{"id":90616,"first_name":"WAN AINI","last_name":"WAN IBRAHIM","domain_name":"teknologimalaysia","page_name":"wanainiwanibrahim","display_name":"WAN AINI WAN IBRAHIM","profile_url":"https://teknologimalaysia.academia.edu/wanainiwanibrahim?f_ri=4656","photo":"https://0.academia-photos.com/90616/1029630/1286661/s65_wan_aini.wan_ibrahim.jpg"}</script></span></span></li><li class="js-paper-rank-work_2411560 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="2411560"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 2411560, container: ".js-paper-rank-work_2411560", }); 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$(".js-view-count[data-work-id=2411560]").text(description); $(".js-view-count-work_2411560").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_2411560").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="2411560"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">16</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="48" href="https://www.academia.edu/Documents/in/Engineering">Engineering</a>, <script data-card-contents-for-ri="48" type="text/json">{"id":48,"name":"Engineering","url":"https://www.academia.edu/Documents/in/Engineering?f_ri=4656","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="923" href="https://www.academia.edu/Documents/in/Technology">Technology</a>, <script data-card-contents-for-ri="923" type="text/json">{"id":923,"name":"Technology","url":"https://www.academia.edu/Documents/in/Technology?f_ri=4656","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="4656" href="https://www.academia.edu/Documents/in/Chromatography">Chromatography</a>, <script data-card-contents-for-ri="4656" type="text/json">{"id":4656,"name":"Chromatography","url":"https://www.academia.edu/Documents/in/Chromatography?f_ri=4656","nofollow":false}</script><a class="InlineList-item-text" data-has-card-for-ri="61603" href="https://www.academia.edu/Documents/in/Uncertainty">Uncertainty</a><script data-card-contents-for-ri="61603" type="text/json">{"id":61603,"name":"Uncertainty","url":"https://www.academia.edu/Documents/in/Uncertainty?f_ri=4656","nofollow":false}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=2411560]'), work: {"id":2411560,"title":"Determination of pesticides in water by cone-shaped membrane protected liquid phase microextraction prior to micro-liquid chromatography","created_at":"2013-01-16T05:10:20.071-08:00","url":"https://www.academia.edu/2411560/Determination_of_pesticides_in_water_by_cone_shaped_membrane_protected_liquid_phase_microextraction_prior_to_micro_liquid_chromatography?f_ri=4656","dom_id":"work_2411560","summary":"A new sample pre-treatment technique termed cone-shaped membrane liquid phase microextraction (CSM-LPME) was developed and combined with micro-liquid chromatography (micro-LC) for the determination of selected pesticides in water samples. Four pesticides (hexaconazole, procymidone, quinalphos and vinclozolin) were considered as target analytes. Several important extraction parameters such as types of extraction solvent, agitation rate, pH value, total exposure time and effect of salt and humic acids were optimized. Enrichment factors of \u003e50 folds were easily achieved within 20 min of extraction. The analytical data demonstrated relative standard deviations for the reproducibility of the optimized CSM-LPME method ranging from 6.3 to 7.5%. The correlation coefficients of the calibration curves were at least 0.9995 across a concentration range of 2–100 μg/L. The detection limits for all the analytes were found to be in the range of 1.1–1.9 μg/L.","downloadable_attachments":[{"id":50639443,"asset_id":2411560,"asset_type":"Work","always_allow_download":false}],"ordered_authors":[{"id":90616,"first_name":"WAN AINI","last_name":"WAN IBRAHIM","domain_name":"teknologimalaysia","page_name":"wanainiwanibrahim","display_name":"WAN AINI WAN IBRAHIM","profile_url":"https://teknologimalaysia.academia.edu/wanainiwanibrahim?f_ri=4656","photo":"https://0.academia-photos.com/90616/1029630/1286661/s65_wan_aini.wan_ibrahim.jpg"}],"research_interests":[{"id":48,"name":"Engineering","url":"https://www.academia.edu/Documents/in/Engineering?f_ri=4656","nofollow":false},{"id":923,"name":"Technology","url":"https://www.academia.edu/Documents/in/Technology?f_ri=4656","nofollow":false},{"id":4656,"name":"Chromatography","url":"https://www.academia.edu/Documents/in/Chromatography?f_ri=4656","nofollow":false},{"id":61603,"name":"Uncertainty","url":"https://www.academia.edu/Documents/in/Uncertainty?f_ri=4656","nofollow":false},{"id":85437,"name":"Pesticides","url":"https://www.academia.edu/Documents/in/Pesticides?f_ri=4656"},{"id":130767,"name":"Humic Substances","url":"https://www.academia.edu/Documents/in/Humic_Substances?f_ri=4656"},{"id":146410,"name":"Humic acid","url":"https://www.academia.edu/Documents/in/Humic_acid?f_ri=4656"},{"id":225787,"name":"High Performance Liquid Chromatography","url":"https://www.academia.edu/Documents/in/High_Performance_Liquid_Chromatography?f_ri=4656"},{"id":260118,"name":"CHEMICAL SCIENCES","url":"https://www.academia.edu/Documents/in/CHEMICAL_SCIENCES?f_ri=4656"},{"id":398287,"name":"Liquid Chromatography","url":"https://www.academia.edu/Documents/in/Liquid_Chromatography?f_ri=4656"},{"id":459764,"name":"Enrichment factor","url":"https://www.academia.edu/Documents/in/Enrichment_factor?f_ri=4656"},{"id":549280,"name":"Reproducibility of Results","url":"https://www.academia.edu/Documents/in/Reproducibility_of_Results?f_ri=4656"},{"id":549972,"name":"Microchemistry","url":"https://www.academia.edu/Documents/in/Microchemistry?f_ri=4656"},{"id":611814,"name":"Correlation 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