Direct Colony Sequencing | GENEWIZ from Azenta

<!DOCTYPE html>    <html lang="en">  <head> <title>Direct Colony Sequencing | GENEWIZ from Azenta</title>  <meta charset="utf-8"> <meta http-equiv="X-UA-Compatible" content="IE=edge,chrome=1"> <meta name="viewport" content="width=device-width, initial-scale=1.0"> <meta name="description" content="GENEWIZ Direct Colony Sequencing services utilize rolling circle amplification (RCA) to enable the Sanger sequencing of bacterial clone or phage sample templates without the need for plasmid preparation."> <meta name="keywords" content="colony sequencing, direct colony sequencing, rca sequencing, rolling circle amplification sequencing, genewiz, azenta life sciences"> <meta name="author" content="">  <link rel="shortcut icon" href="favicon.png">   <link rel="stylesheet" href="/assets/plugins/bootstrap/css/bootstrap.min.css"> <link rel="stylesheet" href="/assets/css/style.css?v=1"> <link rel="stylesheet" href="/assets/css/custom.css?v=1"> <link rel="canonical" href="https://www.genewiz.com/Public/Services/Sanger-Sequencing/Direct-Colony-Sequencing/" /> <link rel="alternate" hreflang="x-default" href="https://www.genewiz.com/Public/Services/Sanger-Sequencing/Direct-Colony-Sequencing" /> <link rel="alternate" hreflang="en-us" href="https://www.genewiz.com/Public/Services/Sanger-Sequencing/Direct-Colony-Sequencing" /> <link rel="alternate" hreflang="en-us" href="https://www.genewiz.com/en/Public/Services/Sanger-Sequencing/Direct-Colony-Sequencing" /> <link rel="alternate" hreflang="en-GB" href="https://www.genewiz.com/en-GB/Public/Services/Sanger-Sequencing/Direct-Colony-Sequencing" /> <script type="text/javascript" src="/sitecore/shell/controls/lib/prototype/prototype.js"></script> <script type="text/javascript" src="/assets/plugins/jquery/jquery.min.js"></script> <script type="text/javascript"> var jQuery = $; //if (window.location.search.indexOf("sc_mode=edit") > 0) { jQuery.noConflict(); //} </script> <script type="text/javascript" src="/assets/plugins/bootstrap/js/bootstrap.min.js"></script> <script type="text/javascript" src="/assets/plugins/back-to-top.js"></script> <script type="text/javascript" src="/assets/js/app.js"></script>   <script> (function (w, d, s, l, i) { w[l] = w[l] || []; w[l].push( { 'gtm.start': new Date().getTime(), event: 'gtm.js' } ); var f = d.getElementsByTagName(s)[0], j = d.createElement(s), dl = l != 'dataLayer' ? 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href='/en/Public/Services/Next-Generation-Sequencing/Standalone-NGS-Solutions' style='color:#585F69;font-size:14px'>Sequencing Only<br></a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Whole-Genome-Sequencing' style='color:#585F69;font-size:14px'>Whole Genome Sequencing</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/High-Throughput-Genotyping' style='color:#585F69;font-size:14px'>CRISPR Validation</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Targeted-Resequencing-Panels' style='color:#585F69;font-size:14px'>Targeted Sequencing</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Metagenomics-Solutions' style='color:#585F69;font-size:14px'>Metagenomics </a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Epigenomics' style='color:#585F69;font-size:14px'>Epigenomics</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Antibody-Discovery' 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Walking</a></li><br><li><a class='GColor' href='/en/Public/Services/Molecular-Genetics' style='color:#826ec3;font-size:17px'>PCR + Sanger Services</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Bacterial-and-Fungal-Identification' style='color:#585F69;font-size:14px'>Bacterial and Fungal Identification</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Mutation-Analysis' style='color:#585F69;font-size:14px'>Mutation Analysis</a></li><li><a href='/en/Public/Services/Molecular-Genetics/SNP-Genotyping' style='color:#585F69;font-size:14px'>SNP Genotyping</a></li><li><a href='/en/Public/Services/Molecular-Genetics/PCR-Plus-Sequencing-CRISPR-Analysis-Package' style='color:#585F69;font-size:14px'>PCR Plus Sequencing CRISPR Analysis Package</a></li><li><a href='/en/Public/Services/Molecular-Genetics/cDNA-Verification' style='color:#585F69;font-size:14px'>Confirmatory Sequencing & Cell Bank Characterization</a></li><li><a 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Whole Genome Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/Regulatory' style='color:#585F69;font-size:14px'>GLP-Compliant Overview</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-Confirmatory-Sequencing' style='color:#585F69;font-size:14px'>GLP Confirmatory Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-SNP-Mutation-Analysis' style='color:#585F69;font-size:14px'>GLP SNP Mutation Analysis</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-Plasmid-Prep' style='color:#585F69;font-size:14px'>GLP Plasmid Prep</a></li><li><a href='/en/Public/Services/Clinical-Services/Nucleic-Acid-Extraction' style='color:#585F69;font-size:14px'>GLP Nucleic Acid Extraction</a></li><li><a href='/en/Public/Services/Clinical-Services/Biofluid-Processing-Overview' style='color:#585F69;font-size:14px'>Biofluid Processing</a></li><li><a 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<div> </div> <p>Direct Colony Sequencing services utilize rolling circle amplification (RCA) to enable Sanger sequencing of bacterial clone or phage sample templates without the need for plasmid preparation. RCA generates DNA for sequencing by random hexamer priming of circular templates. GENEWIZ RCA protocols can handle sequencing projects of any size and are optimized to produce high quality reads from both bacterial colonies and glycerol stocks as well as phage plaques and supernatants. Direct Colony Sequencing is also extremely effective when low amounts of DNA starting material are available (e.g. low copy plasmids).</p> <br /> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://clims4.genewiz.com/SangerSequencing?lobId=1&serviceId=104" target="_blank"><button>Place an Order</button></a> </div> <div class="sanger-content-box" id="sanger-content-box-right"> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://hubs.ly/Q02FKw3H0" target="_blank"><img alt="" src="/-/media/11038-D_PEZ-DirectColonySeq_WebBanRight.ashx?la=en&hash=9E030C709843447C76717D11FCC9D4A59384D0CC" /></a></div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .question { width: 1170px; display: inline-block; } .question p { width: 90%; text-align: left; } .question ul { width: 90%; text-align: left; } @media screen and (max-width: 1170px) { .question { width: auto; } } </style> <div class="question"> <h2>What is colony sequencing?</h2> <p>Colony sequencing is a method in which plasmid DNA (pDNA) is extracted and amplified directly from microbial colonies, allowing for sequencing data to be produced without the need for plasmid purification. In addition to saving time by eliminating plasmid preparation steps, another key benefit of colony sequencing is its flexibility with the starting sample. Any size plasmid, with a high or low copy plasmid template, can be sequenced with direct colony sequencing. Direct colony sequencing is commonly used for screening complimentary DNA (cDNA) libraries and site-directed mutagenesis.</p> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .protocol { width: 1170px; display: inline-block; text-align: center; } .protocol img { width: 80%; height: auto; display: block; margin-left: auto; margin-right: auto; } @media screen and (max-width: 1170px) { .protocol { width: auto; } } </style> <div class="protocol"> <h2>RCA Sequencing Data in 24 Hours**</h2> <br> <p>Using RCA for DNA amplification expedites the Sanger sequencing process and allows your data to be delivered within 24 hours** of sample receipt at Azenta.</p> <br> <a rel="noopener noreferrer" href="https://web.genewiz.com/itr50?_ga=2.88247384.2145649697.1645798714-2103315333.1645541747" target="_blank"><img alt="GENEWIZ AAV-ITR Protocol" src="/-/media/Images/2xDirectColonySequencing2x1.ashx?la=en&hash=F0DB5266CDB06F5ED1FA9AF6D5E5CC9BE18F0FD6"></a> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <div class="container margin-bottom-30 margin-top-50"> <h2>Rolling Circle Amplification Sequencing</h2> <div>RCA was identified in natural systems as a DNA replication mechanism frequently employed in plasmid and viral propagation. When random hexamers are coupled with a strand displacing DNA polymerase, many concatenated template copies are generated with no need for thermocycling (Dean et al., 2001). The synthesized strands are displaced after one round of RCA and are free for additional rounds of primer binding. Exponential amplification is achievable if the RCA reaction contains primers complementary to both template strands</div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .body { text-align: center; display: inline-block; } .banner1 { width: 1170px; background-image: url('/-/media/15002-D_Plasmid-Prep-US_WebBanFull-Med_Background.ashx'); background-size: cover; text-align: left; display: flex; padding: 10px; } .banner1 img { text-align: center; display: block; margin-left: auto; margin-right: auto; } #banner1-left { width: 75%; padding: 30px; } #banner1-right { width: 24%; padding: 30px; } #banner1-left h1 { text-transform: none; letter-spacing: 1px; line-height: 30px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 40px; margin: 0; margin-top: 20px; margin-bottom: 20px; color: #ffffff; } #banner1-left h3 { text-transform: none; letter-spacing: 1px; line-height: 20px; border-bottom: none; font-family: 'Roboto', sans-serif; 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font-weight: 400; font-size: 30px; margin: 0; margin-bottom: 15px; color: #ffffff; } #banner1-left h3 { text-transform: none; letter-spacing: 1px; line-height: 20px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 15px; margin: 0; margin-bottom: 15px; color: #ffffff; } #banner1-left img { width: auto; height: auto; } #banner1-right img { width: 90%; height: auto; } @media only screen and (max-width: 700px) { #banner1-left img { width: 60%; } #banner1-right button { font-size: 14px; } } </style> <div class="body"> <div class="banner1"> <div id="banner1-left"> <h3>HIGHER YIELD</h3> <h1>Scale Up Your Plasmid DNA Preparation</h1> <h3>Now offering up to 5 g yields for high-copy, stable plasmids</h3> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://hubs.ly/Q02qnVRJ0" target="_blank"><button>LEARN MORE</button></a> </div> <div id="banner1-right"> <div id="banner1-right h1"> </div> </div> </div> </div> </div> <div class="process-v1 Image-Slider Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10"> <div class="container"> <div class="headline-center margin-bottom-20"> <div class="row"> <h2>RCA Sequencing</h2> </div> </div> <ul class="list-inline list-st row process-in headline-center"> <li style="text-align:center;cursor:pointer;" class="ImgSlili_1"> <div class="row headline-center"> <img src="/-/media/Icon_172x90_RCA-Step1.ashx?h=91&la=en&w=173&hash=E70E83E8038D132E8B8F64FD811EBF72521FF019" alt="Building" width="173" height="91" /> </div> <div class="row headline-center"> <h3><br></h3> </div> <div class="SeqIcon SeqIcon_1"> <span class="SeqIcon_text">1</span> </div> </li> <li style="vertical-align:bottom;text-align:center;opacity:1" class="Img-arror Img_1"> <img src="/-/media/Gwz/arrow.ashx?h=50&la=en&w=70&hash=E9EE43042F03F59D57F7E4676486569A4310720A" alt="arrow" width="70" height="50" /> </li><li style="text-align:center;cursor:pointer;" class="ImgSlili_2"> <div class="row headline-center"> <img src="/-/media/Icon_172x90_RCA-Step2.ashx?h=91&la=en&w=173&hash=03C69DA0FD16D3698CFF72F23031F8C8714F2ECF" alt="Building" width="173" height="91" /> </div> <div class="row headline-center"> <h3><br></h3> </div> <div class="SeqIcon SeqIcon_2"> <span class="SeqIcon_text">2</span> </div> </li> <li style="vertical-align:bottom;text-align:center;opacity:1" class="Img-arror Img_2"> <img src="/-/media/Gwz/arrow.ashx?h=50&la=en&w=70&hash=E9EE43042F03F59D57F7E4676486569A4310720A" alt="arrow" width="70" height="50" /> </li><li style="text-align:center;cursor:pointer;" class="ImgSlili_3"> <div class="row headline-center"> <img src="/-/media/Icon_172x90_RCA-Step3.ashx?h=91&la=en&w=173&hash=177471036C711A152A0A69E3C792DFC8D6A18526" alt="Building" width="173" height="91" /> </div> <div class="row headline-center"> <h3><br></h3> </div> <div class="SeqIcon SeqIcon_3"> <span class="SeqIcon_text">3</span> </div> </li> <li style="vertical-align:bottom;text-align:center;opacity:1" class="Img-arror Img_3"> <img src="/-/media/Gwz/arrow.ashx?h=50&la=en&w=70&hash=E9EE43042F03F59D57F7E4676486569A4310720A" alt="arrow" width="70" height="50" /> </li><li style="text-align:center;cursor:pointer;" class="ImgSlili_4"> <div class="row headline-center"> <img src="/-/media/Icon_172x90_RCA-Step4.ashx?h=91&la=en&w=173&hash=567566AD72B365D9EFA18EA7ACC6B41B71E9FCEB" alt="Building" width="173" height="91" /> </div> <div class="row headline-center"> <h3><br></h3> </div> <div class="SeqIcon SeqIcon_4"> <span class="SeqIcon_text">4</span> </div> </li> <li style="vertical-align:bottom;text-align:center;opacity:1" class="Img-arror Img_4"> </li> </ul> <div class="row icon-content content_1"> <div class="col-sm-10"> After lysis, bacterial samples are combined with dNTPs, polymerase, and random hexamers. The hexamers anneal to the circular template, and replication initiates at many sites simultaneously.<br> </div> </div><div class="row icon-content content_2"> <div class="col-sm-10"> Polymerase is utilized due to its high stability, processivity, and high fidelity. This allows prolonged strand displacement over multiple rounds of amplification.<br> </div> </div><div class="row icon-content content_3"> <div class="col-sm-10"> As the newly synthesized strands are displaced, a cascade of priming events facilitates exponential amplification of the template.<br /> </div> </div><div class="row icon-content content_4"> <div class="col-sm-10"> Upon completion the sample is ready for Sanger sequencing.<br> </div> </div> </div> <script language="javascript" type="text/javascript"> var i = 1; var iconCount = 0; var icons; var iconContent; var Flag; jQuery(document).ready(function ($) { icons = $(".SeqIcon"); iconContent = $(".icon-content"); //hide the content if (iconContent != null && jQuery(".content_1").length > 0) { iconContent.each(function (i) { if (i > 0) { $(this).hide(); } }); } //change the icon status if (icons != null) { iconCount = icons.length; $(icons[0]).addClass("SeqIcon_Sel"); Flag = setInterval(function () { if (i == iconCount) { i = 0; } SwcRCA(i); i++; }, 2000); } $("li[class^=ImgSlili]").bind("mousemove", function () { clearInterval(Flag); jQuery("div[class^='row icon-content content_']").hide(); jQuery(".Img-arror").find("img").attr("src", "/assets/img/gwz/arrow.png"); jQuery(icons).removeClass("SeqIcon_Sel"); jQuery(".content_" + $(this).attr("class").split("_")[1]).show(); jQuery(icons[$(this).attr("class").split("_")[1] - 1]).addClass("SeqIcon_Sel"); jQuery(".Img_" + $(this).attr("class").split("_")[1]).find("img").attr("src", "/assets/img/gwz/arrow_sel.png"); }); $("li[class^=ImgSlili]").bind("mouseleave", function () { i = 0; Flag = setInterval(function () { SwcRCA(i); i++; }, 2000); jQuery(icons[$(this).attr("class").split("_")[1] - 1]).removeClass("SeqIcon_Sel"); jQuery(".Img_" + $(this).attr("class").split("_")[1]).find("img").attr("src", "/assets/img/gwz/arrow.png"); }); }); function SwcRCA(i) { if (i == 0) { jQuery(icons[icons.length - 1]).removeClass("SeqIcon_Sel"); } else { jQuery(icons[i - 1]).removeClass("SeqIcon_Sel"); } jQuery("div[class^='row icon-content content_']").hide(); jQuery(icons[i]).addClass("SeqIcon_Sel"); jQuery(".content_" + (i + 1)).show(); //change the arrow color jQuery(".Img_" + (i)).find("img").attr("src", "/assets/img/gwz/arrow.png"); if (i != (icons.length - 1)) { jQuery(".Img_" + (i + 1)).find("img").attr("src", "/assets/img/gwz/arrow_sel.png"); } } </script> </div><div class="headline-center Rich-Text-Edit-Area"> <p><br></p> <p><span style="font-size: 10px;">Dean, Frank B. et al. “Rapid Amplification of Plasmid and Phage DNA Using Phi29 DNA Polymerase and Multiply-Primed Rolling Circle Amplification.” Genome Research 11.6 (2001): 1095–1099.</span><br> <br> </p> </div> <script type="text/javascript" src="/assets/plugins/counter/jquery.counterup.min.js"></script> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".row .category").find("img").addClass("img-responsive"); }); </script> <div class='Component-Frame pull-center Multi-column-TextBullet-Layout Component-BackGround-Gray margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="container"> <div class="headline-center"> <h2>Direct Colony Sequencing Features & Benefits</h2> </div> <div class="headline-left"> </div> <div class="row category"> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em class="BlueCheck icon-custom icon-bg-light-gray icon-4x fa fa-check" style="font-size: 50px;"></em></div> <div class="content-boxes-in-v3"> <strong>Time and cost savings:</strong> no need to grow culture and prepare DNA </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em class="BlueCheck icon-custom icon-bg-light-gray icon-4x fa fa-check" style="font-size: 50px;"></em></div> <div class="content-boxes-in-v3"> <b>High-quality data:</b> >600 bases of Phred20 reads </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em class="BlueCheck icon-custom icon-bg-light-gray icon-4x fa fa-check" style="font-size: 50px;"></em></div> <div class="content-boxes-in-v3"> <strong>Project design and consultation:</strong> Ph.D.-level customer support throughout the project </div> </div> </div> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em class="BlueCheck icon-custom icon-bg-light-gray icon-4x fa fa-check" style="font-size: 50px;"></em></div> <div class="content-boxes-in-v3"> <strong>Fast turnaround:</strong> receive your Sanger data within 24 hours** of sample receipt at Azenta </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em class="BlueCheck icon-custom icon-bg-light-gray icon-4x fa fa-check" style="font-size: 50px;"></em></div> <div class="content-boxes-in-v3"> <strong>Online ordering:</strong> 24/7 online ordering and data retrieval system </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em class="BlueCheck icon-custom icon-bg-light-gray icon-4x fa fa-check" style="font-size: 50px;"></em></div> <div class="content-boxes-in-v3"> <strong>GENEWIZ universal primers: </strong>select from a wide range of primers available at no additional cost </div> </div> </div> </div> </div> </div><div class="headline-center Rich-Text-Edit-Area"> **24-hour turnaround time guaranteed for maximum of 96 samples; excludes phage samples </div> <script type="text/javascript" src="/assets/plugins/counter/jquery.counterup.min.js"></script> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".row .category").find("img").addClass("img-responsive"); }); </script> <div class='Component-Frame pull-center Multi-column-TextBullet-Layout Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="container"> <div class="headline-center"> <h2>RCA Sequencing Applications</h2> </div> <div class="headline-left"> </div> <div class="row category"> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <p>shRNA cloning and library screening; Eliminate plasmid preps and sequence colonies directly.</p> </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <p>DNA methylation analysis: Save time & obtain high-quality reads by cloning PCR products directly and sequencing the bacterial colonies.</p> </div> </div> </div> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <p>Antibody phage display library validation: Direct phage sequencing for faster screening and validation.</p> </div> </div> </div> </div> </div> </div><div class="headline-center Rich-Text-Edit-Area"> <link href="https://fonts.googleapis.com/css?family=Roboto:300,400,500,700" rel="stylesheet" /> <style type="text/css"> .covid-banner1 { width: 1170px; height: auto; margin: 0 auto; background-image: url("/-/media/11036-D_Plasmid-ONT_WebBanFull-Med_Background.ashx"); background-size: cover; background-position: center; text-align: left; padding: 0px 0 0px 0; } .covid-content1 { text-align: center; } .covid-box1 h3 { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 450; line-height: 125%; font-size: 35px; margin: 0; padding-bottom: 15px; padding-top: 25px; } .covid-box1 h4 { color: #ffffff; text-transform: none; letter-spacing: 1px; line-height: 30px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 20px; margin: 0; margin-bottom: 5px; } .covid-box1 p { color: #000000; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 15px; margin: 0; margin-bottom: 5px; } .covid-box1 button { padding: 8px 19px; color: #826ec3; letter-spacing: 1px; background: #ffffff; border: 2px solid #ffffff; border-radius: 0px; transition: all .2s ease-in-out; font-size: 15px } .covid-box1 button:hover { cursor: pointer; transform: scale(1.1); } .covid-box1 button:focus { outline: 0; } @media only screen and (max-width: 1170px) { .covid-banner1 { width: 100%; height: auto; } .covid-content1 { width: 90%; margin: 0 auto; } } </style> <br /> <div class="covid-banner1"> <div class="covid-content1"> <div class="covid-box1"> <br /> <h3>Ensure Complete Plasmid Validation on Your First Attempt</h3> <h4>with Oxford Nanopore Sequencing</h4> <a href="https://hubs.ly/Q02BX3QZ0" target="_blank"> <br /> <button> READ BLOG</button> <br /> </a> <p> </p> </div> </div> </div> <p> </p> </div> <div class="headline-center Rich-Text-Edit-Area"> <h2>SAMPLE SUBMISSION OPTIONS</h2> <div class="container text-left"> <div style="width: 80%; margin: 0px auto;" class="panel panel-purple margin-bottom-40"> <div class="panel-heading"> <h3 class="panel-title">Sample Submission Options</h3> </div> <table class="table"> <tbody> <tr> <th class="col-md-3">Glycerol Stock* </th> <td>Send 1.5 ml tubes or 96-well plates; samples must be shipped on dry ice. Please label with media type. </td> </tr> <tr> <th class="col-md-3">Bacterial Colonies*</th> <td>Array colonies in a grid on the agar plate and circle the colonies requiring sequencing. Numerically label each colony. Note that unnumbered plates will be processed as random pick. If using liquid culture, submit samples in 96-well plate with strip caps. Wrap agar plates in parafilm, and provide a cushion to protect against potential damage.</td> </tr> <tr> <th class="col-md-3">Phage Supernatant*</th> <td>Please submit 50 ul of phage supernatant in 8-strip PCR tubes or 1.5 ml microcentrifuge tubes clearly labeled with sample name. </td> </tr> <tr> <th class="col-md-3">Phage Plaque</th> <td>Circle and number the plaques you submit for sequencing. For transport, wrap agar plates in parafilm and provide cushion to protect against potential damage.</td> </tr> </tbody> </table> </div> </div> <p>*Please note: RCA protocol is intended for circular templates <10 kb; linear templates will not be amplified sufficiently for direct Sanger sequencing. <br> Longer circular templates, or circular templates carried in bacterial strains with functional endA may not be reliably amplified, contributing to variable results</p> <br> <p>Please visit our <a href="/Public/Resources/Sample-Submission-Guidelines/Sanger-Sequencing-Sample-Submission-Guidelines">Sample Submission Guidelines</a> for bacteria and phage templates for additional details.</p><p><br></p> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> body { margin: 0; } .tech-resources-container { width: 1170px; margin: 0 auto; } .tech-resources-container h2 { text-align: center; border-bottom: none; } .tech-resource { display: flex; align-items: center; flex-direction: row; margin-bottom: 35px; } .resource { flex-grow: 1; } .resource-right h3, .resource-right p { width: 90%; margin: 0 auto; border-bottom: none; text-align: left; } .resource button { padding: 5px 15px; border: none; border-radius: 4px; background: #826ec3; color: #fff; font-size: 15px; font-weight: lighter; letter-spacing: 1px; margin-top: 15px; -webkit-transition: ease-in-out 0.2s; -moz-transition: ease-in-out 0.2s; -o-transition: ease-in-out 0.2s; transition: ease-in-out 0.2s; } .resource button:hover { background: #003d6a; } @media only screen and (max-width: 800px) { .tech-resource { flex-direction: column; text-align: center; } .resource-right h3, .resource-right p { text-align: center; } } @media only screen and (max-width: 1170px) { .tech-resources-container { width: 80%; margin: 0 auto; } } </style> <div style="width: 100%; background-color: #ffffff; padding: 0; margin: 0;margin-top: -20px;"> <h2 style="border-bottom: 1px solid #f1f0f0;">TECHNICAL RESOURCES</h2> <div class="tech-resources-container"> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/Sanger-Quick-Tips.ashx?h=160&w=220&la=en&hash=10DBA6E5F7939B892C9441028D5B83BD5B69682C" style="width: 220px; height: 160px;" /> </div> <div class="resource resource-right"> <h3>Sanger Sequencing Quick Tips Guide</h3> <p>Learn from the Sanger experts how you can get the most of your DNA Sequencing. Whether you're troubleshooting a tough sequencing reaction, perfecting PCR visualization, or learning to clean up and refine your PCR products, these resources are designed to help you get the best results.<br /> • Volume 1: Producing Robust, Single-band PCR Product<br /> • Volume 2: PCR Clean-up of Single-band PCR <br /> • Volume 3: Salvaging Nonspecific PCR Products <br /> • Volume 4: PCR Visualization by Gel Electrophoresis<br /> • Volume 5: Troubleshooting a Bad Sequencing Reaction from a PCR Template <br /> <a href="https://web.genewiz.com/quick-tips-guide/sanger-sequencing" target="_blank"> <button>Download Now</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/Sanger-Trouble-shooting-blog.ashx?h=160&w=220&la=en&hash=24FECB44F887ED8D2D363153D5842A55828C120E" style="width: 220px; height: 160px;" /> </div> <div class="resource resource-right"> <h3>Blog | Troubleshooting DNA Templates with Sanger Sequencing</h3> <p>Sanger sequencing is highly effective for testing small targeted genomic regions and for validating results from NGS. However, as with all sequencing technologies, there are limitations and needs for troubleshooting. Read on to learn about the three basic troubleshooting steps we recommend to start with.<br /> <a href="https://www.azenta.com/learning-center/blog/troubleshooting-dna-templates-sanger-sequencing" target="_blank"> <button>Read Now</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/Webinar--Sanger-ONT.ashx?h=160&w=220&la=en&hash=B46A4AA56DE351DD45E75213BD6FD1AA5AB8F799" style="width: 220px; height: 160px;" /> </div> <div class="resource resource-right"> <h3>Webinar | From Base Pairs to Breakthroughs: Understanding Sanger and Oxford Nanopore Sequencing</h3> <p>While Sanger sequencing remains the gold standard for accurate DNA sequencing, advancements in sequencing technology, such as Oxford Nanopore Technology (ONT), have provided a cost-effective, long-read alternative for analysis. In this workshop, delve into the considerations of each technique and discover how to interpret their resulting sequencing data effectively so you can capture the benefits of both approaches for your specific needs. <br /> <a href="https://web.genewiz.com/workshop/base-pairs-to-breakthroughs" target="_blank"> <button>Watch Now</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/Analyzing-Sanger-Blog.ashx?h=160&w=220&la=en&hash=8A9D75A95F2026E45E36D46F1028677640443783" style="width: 220px; height: 160px;" /> </div> <div class="resource resource-right"> <h3>Blog | Analyzing Sanger Sequencing Data</h3> <p>The output for Sanger sequencing is typically a chromatogram, also known as a trace or ab1 file, and a text-based sequence file. Although the latter may seem to hold all the relevant information—after all, the point of sequencing is to get a sequence—the former can't be ignored. Here, we provide a guide to understanding Sanger sequencing data. <br /> <a href="https://www.azenta.com/learning-center/blog/analyzing-sanger-sequencing-data" target="_blank"> <button>Read Now</button> </a> </p> </div> </div> <p> </p> </div> </div> </div>  <div class="process-v1 How-To-Order Component-BackGround-Gray margin-top-0 padding-top-10 padding-bottom-10 "> <div class=" container"> <div class="headline-center"> <div class="row"> <h2>HOW TO ORDER</h2> </div> <div class="row"> <p>*Samples must arrive at the GENEWIZ New Jersey laboratory before 10:00 am EST to qualify for Same Day service.  Note that direct-sequencing templates are not available for our Same Day service.</p> <br> </div> </div> <ul class="list-inline row process-in process-border-4 "> <li class="col-sm-3"> <div class="process-icon"> <i class="rounded-x dot-active"></i> </div> <a href='https://clims4.genewiz.com/RegisterAccount/Index ' target='_blank'> <div class="process-desc"> <img 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