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Off-Gel Protein Sequencing
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href="/contact">contact us</a> </span> <span class="Header-showOnSticky u-padLeft2"> <a class="Button u-lg-hide" href="/contact">contact us</a> </span> </div> </div> </div> </div> </header> <div class="TextHeader"> <div class="Container"> <div class="TextHeader-content"> <h1 class="TextHeader-title u-redlineLeft">Gel-Based Protein Sequencing</h1> </div> </div> </div> <main> <div class="Container"> <div class="Content u-cf"> <table class="Table--themed" style="border-collapse: collapse; width: 99.9211%;" border="1"> <thead> <tr> <th style="width: 32.8069%;" scope="col">Application</th> <th style="width: 32.8069%;" scope="col">Service</th> <th style="width: 32.8076%;" scope="col">Description</th> </tr> </thead> <tbody> <tr> <td style="width: 32.8069%;">Gel-based sequencing</td> <td style="width: 32.8069%;"> <div>Label free</div> <div><a href="/services/tmtcalibrator-workflow">TMTcalibrator</a></div> <div><a href="/services/tmt-ms2-workflow">TMT LC-MS2</a></div> <div><a href="/services/tmt-ms3-workflow">TMT LC-MS3</a></div> </td> <td style="width: 32.8076%;"> <ul> <li>Identification of proteins from discrete regions in SDS-PAGE and non-denaturing gels</li> <li>Part of unbiased discovery or enrichment of target proteins prior to SRM or PRM analysis</li> <li>Can be used for biotherapeutic analysis</li> </ul> </td> </tr> </tbody> </table> <h2>Advantages</h2> <ul> <li>Detection and quantification of proteins from a 1D or 2D gel band</li> <li>Human, rodent, monkey, pig and others all covered</li> <li>Compatibility with a wide variety of sample matrices, including tissue, serum, cell lysates, CSF, exosomes, etc.</li> <li>Includes full computational proteomic analysis and bioinformatics </li> </ul> <h2>Protein Identification</h2> <p>Proteome Sciences offers this service to enable the identification of proteins in a gel band or 2-dimensional gel spot. Generally this is designed for laboratories that have already run gels, but we can also run standard SDS-PAGE and non-denaturing 1- and 2-dimensional gels if required.</p> <p>For the greatest sensitivity towards your target you should cut the band close to the expected molecular weight region. Please note; gels should only be stained with mass spectrometry-compatible stains. Typical gel band dimensions will be ~5mm x 2mm. We can get an identification with as little as 1ng of protein on gel. Generally we would use a Label Free approach and a Orbitrap Mass Spectrometer, but for multiple samples where more precise quantification is required, different samples can be TMT labelled.</p> <p>We can provide similar services for isolated proteins in solution e.g. following LC or capillary electrophoresis. We recommend submitting samples at a protein concentration of ≥1µg/µL in 10µL of buffer. As some detergents and other buffers can affect the performance of the Mass Spectrometer or interfere with the assay, please call/email so we can discuss before you plan sample preparation</p> <h2>Steps Involved</h2> <ul> <li>Sample preparation: In-gel digestion with trypsin or another more specific protease according to assay needs</li> <li>Mass Spectrometry analysis: Digested samples are analysed with 0.5hr LC/MS2 runs</li> <li>Data analysis: Protein identification is performed with Proteome Discoverer</li> </ul> <p>The turnaround time is typically 2-3 weeks.</p> <h2>Deliverables</h2> <ul> <li>Report generation: will be PowerPoint/Word document listing the proteins detected and the raw MS data </li> <li>An Excel workbook containing the detailed qualitative and quantitative protein identification data.</li> </ul> <table class="Table--themed" style="border-collapse: collapse; width: 99.9211%; height: 75px;" border="1"> <tbody> <tr style="height: 25px;"> <td style="width: 49.4672%; height: 25px;">What’s included</td> <td style="width: 49.4672%; height: 25px;"> <ul> <li>Incoming sample QC</li> <li>Protein capture/elution, TMT labelling unless label free analysis</li> <li>Mass spectrometry-based peptide and protein ID</li> <li>Bioinformatics</li> <li>Report discussing the proteins sequenced in the form of a PowerPoint presentation</li> <li>An Excel based QuantSheet detailing qualitative and quantitative protein identification data.</li> </ul> </td> </tr> <tr style="height: 25px;"> <td style="width: 49.4672%; height: 25px;">Material required</td> <td style="width: 49.4672%; height: 25px;"> <ul> <li>This is sample and assay specific and will be detailed in the agreed Statement of Work - please <a href="/contact">contact us</a> to discuss your specific needs so we can start the Statement of Work preparation</li> </ul> </td> </tr> <tr style="height: 25px;"> <td style="width: 49.4672%; height: 25px;">Typical turnaround time</td> <td style="width: 49.4672%; height: 25px;"> <ul> <li>Generally 2 to 3 weeks from receipt of samples and completion of incoming sample QC </li> </ul> </td> </tr> </tbody> </table> </div> </div> </main> <footer class="Footer"> <div class="Footer-upper"> <div class="Container"> <div class="Grid Grid--fit Grid--alignMiddle"> <div class="Grid-cell u-sizeAuto u-lg-hide"> <a href="/" title="Back to home page"> <img class="Footer-logo" src="https://files.aptuitivcdn.com/JxEdylMPMz-1515/theme/custom/images/logo-white.1654711795.svg" alt="Proteome Sciences"> </a> </div> <div class="Grid-cell u-lg-sizeFull u-lg-textCenter"> <ul class="FooterNav"> <li class="FooterNav-item is-first"> <a href="/about" class="is-first FooterNav-link">About Us</a> </li> <li class="FooterNav-item is-current"> <a href="/services" class="is-current FooterNav-link">Proteomic Services</a> </li> <li class="FooterNav-item"> <a href="/news" class="FooterNav-link">News</a> </li> <li class="FooterNav-item"> <a href="/investors" class="FooterNav-link">Investors</a> </li> <li class="FooterNav-item"> <a href="/contact" class="FooterNav-link">Contact</a> </li> <li class="FooterNav-item is-last"> <a href="/privacy-notice" class="is-last FooterNav-link">Privacy Notice</a> </li> </ul> </div> <div class="Grid-cell u-sizeAuto u-lg-size1of2 u-textRight u-lg-textCenter u-sm-sizeFull u-sm-margBottom4"> <img src="https://files.aptuitivcdn.com/JxEdylMPMz-1515/images/ISO-9001-2.2015.jpg" height="125" width="150" alt="DQS Quality Management" class="Footer-badge"> <a href="https://twitter.com/ProteomeScience" title="Follow us on Twitter" class="Footer-iconLink" target="_blank" rel="noopenner noreferrer"> <svg class="Footer-icon Icon" role="img" alt="Twitter" aria-labelledby="iconTitle-fpdjbY" focusable="false"><title id="iconTitle-fpdjbY">Twitter</title><use xlink:href="#icon-twitter" /></svg></a> <a href="https://www.linkedin.com/company/proteome-sciences-plc" title="Follow us on LinkedIn" class="Footer-iconLink" target="_blank" rel="noopenner noreferrer"> <svg class="Footer-icon Icon" role="img" alt="LinkedIn" aria-labelledby="iconTitle-SyD6Jm" focusable="false"><title id="iconTitle-SyD6Jm">LinkedIn</title><use xlink:href="#icon-linkedin" /></svg></a> </div> </div> </div> </div> <div class="Container u-spaced1 u-gutters1"> <div class="Grid Grid--fit Grid--alignMiddle"> <div class="Grid-cell u-sizeAuto u-md-size1of2 u-md-textCenter u-sm-sizeFull u-sm-margBottom1 u-darkerText">Copyright © Proteome Sciences 2024</div> <div class="Grid-cell u-md-size1of2 u-md-textCenter u-sm-sizeFull u-darkerText"></div> <div class="Grid-cell u-sizeAuto u-md-sizeFull u-md-textCenter"> <div class="Footer-credit u-inlineBlock"> <a href="https://www.aptuitiv.com" target="_blank" class="Footer-link Footer-creditLink">Site Design By Aptuitiv</a>-<a href="https://www.branchcms.com" target="_blank" class="Footer-link Footer-creditLink">Powered by BranchCMS</a> </div> </div> </div> </div> </footer> <script> tarteaucitron.services.recaptchav3 = { "key": "recaptchav3", "type": "analytic", "name": "reCAPTCHA", "needConsent": true, "cookies": ['_GRECAPTCHA', 'AEC'], "js": function () { "use strict"; 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