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class="u-taCenter"></div><div class="profile--tab_content_container js-tab-pane tab-pane active" id="all"><div class="profile--tab_heading_container js-section-heading" data-section="Papers" id="Papers"><h3 class="profile--tab_heading_container">Papers by Marshall Porterfield</h3></div><div class="js-work-strip profile--work_container" data-work-id="53418004"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/53418004/Oxygen_consumption_oscillates_in_single_clonal_pancreatic_beta_cells_HIT_"><img alt="Research paper thumbnail of Oxygen consumption oscillates in single clonal pancreatic beta-cells (HIT)" class="work-thumbnail" src="https://attachments.academia-assets.com/70271947/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/53418004/Oxygen_consumption_oscillates_in_single_clonal_pancreatic_beta_cells_HIT_">Oxygen consumption oscillates in single clonal pancreatic beta-cells (HIT)</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://upenn.academia.edu/BarbaraCorkey">Barbara Corkey</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/MarshallPorterfield1">Marshall Porterfield</a></span></div><div class="wp-workCard_item"><span>Diabetes</span><span>, 2000</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="bc98e0aa662f38603b66f7f8aa00fce9" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" 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in Ca 2+. It is well known from single-cell Ca 2+ studies that variable oscillatory patterns in Ca 2+ occur in response to glucose stimulation. The present studies, using the clonal ␤-cell line HIT-T-15, were undertaken to evaluate the relationship between glucose concentration, insulin secretion, and O 2 consumption and to determine the Ca 2+ dependency of glucose-induced changes in O 2 consumption. In population studies, an excellent correlation was found between respiration and insulin secretion, with half-maximal values at 1 mmol/l glucose for both respiration and secretion. In the absence of Ca 2+ , glucose stimulated O 2 consumption but not insulin secretion. In single clonal ␤-cells, a selfreferencing O 2 electrode was used to assess O 2 consumption. Large-amplitude oscillations were found to occur in response to stimulation by glucose and were blocked by uncoupling respiration with carbonylcyanide p-(trifluoromethoxy)phenylhydrazone (FCCP). They were also blocked and respiration totally inhibited by antimycin A, an inhibitor of complex III of the respiratory chain. Half of the cells sampled (~100 total) exhibited increased oscillatory O 2 consumption in response to glucose. Oscillations in O 2 occurred in response to glucose even in the absence of Ca 2+ , and their amplitude increased further on restoration of a normal extracellular Ca 2+ level. These studies indicated that oscillatory O 2 consumption was not dependent on Ca 2+ but that the amplitude of the O 2 oscillations increased in the presence of Ca 2+ , possibly reflecting the additional work involved in insulin secretion and Ca 2+ pumping. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="123106082"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/123106082/Aging_and_putative_frailty_biomarkers_are_altered_by_spaceflight"><img alt="Research paper thumbnail of Aging and putative frailty biomarkers are altered by spaceflight" class="work-thumbnail" src="https://attachments.academia-assets.com/117619558/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/123106082/Aging_and_putative_frailty_biomarkers_are_altered_by_spaceflight">Aging and putative frailty biomarkers are altered by spaceflight</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Human space exploration is hazardous, causing molecular changes that can alter astronauts&#39; he...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Human space exploration is hazardous, causing molecular changes that can alter astronauts&#39; health. This can include genomic instability, mitochondrial dysfunction, increased inflammation, homeostatic dysregulation, and epigenomic changes. These alterations are similar to changes during aging on Earth. However, little is known about the link between these changes and disease development in space. Frailty syndrome is a robust predictor associated with biological aging, however its existence during spaceflight has not been examined. We used murine data from NASA’s GeneLab and astronaut data from JAXA and Inspiration4 missions to evaluate the presence of biological markers and pathways related to frailty, aging and sarcopenia. We identified changes in gene expression that could be related to the development of a frailty-like condition. These results suggest that the parallels between spaceflight and aging may extend to frailty as well. Future studies examining the utility of a frail...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="3d24459106eb4ce0fd677dacb7f265d6" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":117619558,"asset_id":123106082,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/117619558/download_file?st=MTczMjg0MTM3MCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="123106082"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="123106082"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 123106082; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=123106082]").text(description); $(".js-view-count[data-work-id=123106082]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 123106082; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='123106082']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 123106082, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "3d24459106eb4ce0fd677dacb7f265d6" } } $('.js-work-strip[data-work-id=123106082]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":123106082,"title":"Aging and putative frailty biomarkers are altered by spaceflight","translated_title":"","metadata":{"abstract":"Human space exploration is hazardous, causing molecular changes that can alter astronauts\u0026#39; health. This can include genomic instability, mitochondrial dysfunction, increased inflammation, homeostatic dysregulation, and epigenomic changes. These alterations are similar to changes during aging on Earth. However, little is known about the link between these changes and disease development in space. Frailty syndrome is a robust predictor associated with biological aging, however its existence during spaceflight has not been examined. We used murine data from NASA’s GeneLab and astronaut data from JAXA and Inspiration4 missions to evaluate the presence of biological markers and pathways related to frailty, aging and sarcopenia. We identified changes in gene expression that could be related to the development of a frailty-like condition. These results suggest that the parallels between spaceflight and aging may extend to frailty as well. 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These results suggest that the parallels between spaceflight and aging may extend to frailty as well. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="123106080"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/123106080/Extraterrestrial_Gynecology_Could_Spaceflight_Increase_the_Risk_of_Developing_Cancer_in_Female_Astronauts_An_Updated_Review"><img alt="Research paper thumbnail of Extraterrestrial Gynecology: Could Spaceflight Increase the Risk of Developing Cancer in Female Astronauts? An Updated Review" class="work-thumbnail" src="https://attachments.academia-assets.com/117619538/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/123106080/Extraterrestrial_Gynecology_Could_Spaceflight_Increase_the_Risk_of_Developing_Cancer_in_Female_Astronauts_An_Updated_Review">Extraterrestrial Gynecology: Could Spaceflight Increase the Risk of Developing Cancer in Female Astronauts? An Updated Review</a></div><div class="wp-workCard_item"><span>International Journal of Molecular Sciences</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Outer space is an extremely hostile environment for human life, with ionizing radiation from gala...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Outer space is an extremely hostile environment for human life, with ionizing radiation from galactic cosmic rays and microgravity posing the most significant hazards to the health of astronauts. Spaceflight has also been shown to have an impact on established cancer hallmarks, possibly increasing carcinogenic risk. Terrestrially, women have a higher incidence of radiation-induced cancers, largely driven by lung, thyroid, breast, and ovarian cancers, and therefore, historically, they have been permitted to spend significantly less time in space than men. In the present review, we focus on the effects of microgravity and radiation on the female reproductive system, particularly gynecological cancer. The aim is to provide a summary of the research that has been carried out related to the risk of gynecological cancer, highlighting what further studies are needed to pave the way for safer exploration class missions, as well as postflight screening and management of women astronauts foll...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="fe52c86405fd16c6aeb4662283ee9285" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":117619538,"asset_id":123106080,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/117619538/download_file?st=MTczMjg0MTM3MCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="123106080"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="123106080"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 123106080; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=123106080]").text(description); $(".js-view-count[data-work-id=123106080]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 123106080; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='123106080']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 123106080, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "fe52c86405fd16c6aeb4662283ee9285" } } $('.js-work-strip[data-work-id=123106080]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":123106080,"title":"Extraterrestrial Gynecology: Could Spaceflight Increase the Risk of Developing Cancer in Female Astronauts? 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="123106079"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/123106079/A_Ground_based_Comparison_of_Nutrient_Delivery_Technologies_Originally_Developed_for_Growing_Plants_in_the_Spaceflight_Environment"><img alt="Research paper thumbnail of A Ground-based Comparison of Nutrient Delivery Technologies Originally Developed for Growing Plants in the Spaceflight Environment" class="work-thumbnail" src="https://attachments.academia-assets.com/117619539/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/123106079/A_Ground_based_Comparison_of_Nutrient_Delivery_Technologies_Originally_Developed_for_Growing_Plants_in_the_Spaceflight_Environment">A Ground-based Comparison of Nutrient Delivery Technologies Originally Developed for Growing Plants in the Spaceflight Environment</a></div><div class="wp-workCard_item"><span>HortTechnology</span><span>, 2000</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">A ground-based comparison of plant nutrient delivery systems that have been developed for microgr...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">A ground-based comparison of plant nutrient delivery systems that have been developed for microgravity application was conducted for dwarf wheat (Triticum aestivum L. `Yecora Rojo&#39;) and rapid-cycling brassica (Brassica rapa L. CrGC#1-33) plants. These experiments offer insight into nutrient and oxygen delivery concerns for greenhouse crop production systems. The experiments were completed over a 12-day period to simulate a typical space shuttle-based spaceflight experiment. The plant materials, grown either using the porous-tube nutrient delivery system, the phenolic foam support system, or a solidified agar nutrient medium, were compared by plant-growth analysis, root zone morphological measurements, elemental composition analysis, and alcohol dehydrogenase enzyme activity assay. The results of these analyses indicate that the porous tube plant nutrient delivery and the phenolic foam systems maintain plant growth at a higher level than the solidified agar gel medium system. Roo...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="2616932da329ae4984b8e0243e91cd86" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":117619539,"asset_id":123106079,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/117619539/download_file?st=MTczMjg0MTM3MCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="123106079"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="123106079"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 123106079; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=123106079]").text(description); $(".js-view-count[data-work-id=123106079]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 123106079; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='123106079']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 123106079, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "2616932da329ae4984b8e0243e91cd86" } } $('.js-work-strip[data-work-id=123106079]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":123106079,"title":"A Ground-based Comparison of Nutrient Delivery Technologies Originally Developed for Growing Plants in the Spaceflight Environment","translated_title":"","metadata":{"abstract":"A ground-based comparison of plant nutrient delivery systems that have been developed for microgravity application was conducted for dwarf wheat (Triticum aestivum L. `Yecora Rojo\u0026#39;) and rapid-cycling brassica (Brassica rapa L. 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class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/123106078/Proteins_and_lipids_define_the_diffusional_field_of_nitric_oxide">Proteins and lipids define the diffusional field of nitric oxide</a></div><div class="wp-workCard_item"><span>American Journal of Physiology-Lung Cellular and Molecular Physiology</span><span>, 2001</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Nitric oxide (NO) fluxes released from the surface of individual activated macrophages or cells l...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Nitric oxide (NO) fluxes released from the surface of individual activated macrophages or cells localized in small aggregates were measured with a novel polarographic self-referencing microsensor. NO fluxes could be detected at distances from the cells of 100–500 μm. The initial flux and the distance from the cells at which NO could be detected were directly related to the number of cells in the immediate vicinity of the probe releasing NO. Thus, whereas NO fluxes of ∼1 pmol · cm−2· s−1were measured from individual macrophages, aggregates composed of groups of cells varying in number from 18 to 48 cells produced NO fluxes of between ∼4 and 10 pmol · cm−2· s−1. NO fluxes required the presence of l-arginine. Signals were significantly reduced by the addition of hemoglobin and by N-nitro-l-arginine methyl ester. NO fluxes were greatest when the sensor was placed immediately adjacent to cell membranes and declined as the distance from the cell increased. The NO signal was markedly reduc...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="7c003b7e907abdf530a118a403c78c7a" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":117619536,"asset_id":123106078,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/117619536/download_file?st=MTczMjg0MTM3MCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="123106078"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="123106078"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 123106078; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=123106078]").text(description); $(".js-view-count[data-work-id=123106078]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 123106078; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='123106078']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 123106078, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "7c003b7e907abdf530a118a403c78c7a" } } $('.js-work-strip[data-work-id=123106078]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":123106078,"title":"Proteins and lipids define the diffusional field of nitric oxide","translated_title":"","metadata":{"abstract":"Nitric oxide (NO) fluxes released from the surface of individual activated macrophages or cells localized in small aggregates were measured with a novel polarographic self-referencing microsensor. 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fern spores</a></div><div class="wp-workCard_item"><span>Lab on a Chip</span><span>, 2017</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">A device designed for space flight measures the longest continuous observation of a plant cell&#3...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">A device designed for space flight measures the longest continuous observation of a plant cell&#39;s calcium response to hypergravity made to date.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper 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Non-invasive detection of ascorbate fluxes in indivdual, oxidatively-stressed early embryos" class="work-thumbnail" src="https://attachments.academia-assets.com/117619556/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/123106075/P_157_Non_invasive_detection_of_ascorbate_fluxes_in_indivdual_oxidatively_stressed_early_embryos">P-157. Non-invasive detection of ascorbate fluxes in indivdual, oxidatively-stressed early embryos</a></div><div class="wp-workCard_item"><span>Human Reproduction</span><span>, 1999</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="91cf5531c006b4f43391c396250293a6" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":117619556,"asset_id":123106075,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/117619556/download_file?st=MTczMjg0MTM3MCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="123106075"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="123106075"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 123106075; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=123106075]").text(description); $(".js-view-count[data-work-id=123106075]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 123106075; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='123106075']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 123106075, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "91cf5531c006b4f43391c396250293a6" } } $('.js-work-strip[data-work-id=123106075]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":123106075,"title":"P-157. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="123106072"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/123106072/An_assessment_of_US_microbiome_research"><img alt="Research paper thumbnail of An assessment of US microbiome research" class="work-thumbnail" src="https://attachments.academia-assets.com/117619554/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/123106072/An_assessment_of_US_microbiome_research">An assessment of US microbiome research</a></div><div class="wp-workCard_item"><span>Nature Microbiology</span><span>, 2016</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="a6f822116b698726d8a40bbfc8b0331c" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":117619554,"asset_id":123106072,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/117619554/download_file?st=MTczMjg0MTM3MCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="123106072"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="123106072"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 123106072; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="123106071"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/123106071/Non_invasive_measurement_of_real_time_oxygen_flux_in_plant_systems_with_a_self_referencing_optrode"><img alt="Research paper thumbnail of Non-invasive measurement of real-time oxygen flux in plant systems with a self-referencing optrode" class="work-thumbnail" src="https://attachments.academia-assets.com/117619553/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/123106071/Non_invasive_measurement_of_real_time_oxygen_flux_in_plant_systems_with_a_self_referencing_optrode">Non-invasive measurement of real-time oxygen flux in plant systems with a self-referencing optrode</a></div><div class="wp-workCard_item"><span>Protocol Exchange</span><span>, 2011</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="1c83964ecc5e14021e76ca43b9bc0559" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":117619553,"asset_id":123106071,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/117619553/download_file?st=MTczMjg0MTM3MCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="123106071"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="123106071"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 123106071; 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="123106070"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/123106070/New_developments_in_electronic_reference_controls_for_frequency_domain_optical_sensing"><img alt="Research paper thumbnail of New developments in electronic reference controls for frequency domain optical sensing" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/123106070/New_developments_in_electronic_reference_controls_for_frequency_domain_optical_sensing">New developments in electronic reference controls for frequency domain optical sensing</a></div><div class="wp-workCard_item"><span>Smart Biomedical and Physiological Sensor Technology VI</span><span>, 2009</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">The reference optical path is essential for optical systems which function on the basis of light ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">The reference optical path is essential for optical systems which function on the basis of light interference. In the case of frequency domain (FD) fluorescence life-time optrodes, a reference LED is used as a standard for calculating the phase angle. The reference LED is configured so that radiation travels the same length to the detector as that of the fluorescence</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="123106070"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="123106070"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 123106070; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=123106070]").text(description); $(".js-view-count[data-work-id=123106070]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 123106070; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='123106070']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 123106070, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (false){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "-1" } } $('.js-work-strip[data-work-id=123106070]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":123106070,"title":"New developments in electronic reference controls for frequency domain optical sensing","translated_title":"","metadata":{"abstract":"The reference optical path is essential for optical systems which function on the basis of light interference. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="123106069"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/123106069/A_New_Microsensor_System_for_Plant_Root_Zone_Monitoring"><img alt="Research paper thumbnail of A New Microsensor System for Plant Root Zone Monitoring" class="work-thumbnail" src="https://attachments.academia-assets.com/117619552/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/123106069/A_New_Microsensor_System_for_Plant_Root_Zone_Monitoring">A New Microsensor System for Plant Root Zone Monitoring</a></div><div class="wp-workCard_item"><span>IEEE Sensors, 2005.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="5a570b50ea65797d2a5a951abcdf0767" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":117619552,"asset_id":123106069,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/117619552/download_file?st=MTczMjg0MTM3MCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="123106069"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="123106069"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 123106069; 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class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" rel="nofollow" href="https://www.academia.edu/123106068/Contributor_contact_details"><img alt="Research paper thumbnail of Contributor contact details" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" rel="nofollow" href="https://www.academia.edu/123106068/Contributor_contact_details">Contributor contact details</a></div><div class="wp-workCard_item"><span>Biosensors for Medical Applications</span><span>, 2012</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span 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"profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="123106067"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/123106067/Self_referencing_optrode_technology_for_non_invasive_real_time_measurement_of_biophysical_flux_and_physiological_sensing"><img alt="Research paper thumbnail of Self-referencing optrode technology for non-invasive real-time measurement of biophysical flux and physiological sensing" class="work-thumbnail" src="https://attachments.academia-assets.com/117619551/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/123106067/Self_referencing_optrode_technology_for_non_invasive_real_time_measurement_of_biophysical_flux_and_physiological_sensing">Self-referencing optrode technology for non-invasive real-time measurement of biophysical flux and physiological sensing</a></div><div class="wp-workCard_item"><span>The Analyst</span><span>, 2009</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="fbe28f2e2dee32a3017f44b0ac1b6950" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":117619551,"asset_id":123106067,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/117619551/download_file?st=MTczMjg0MTM3MCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: 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class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/123106066/Multitip_scanning_bio_Kelvin_probe"><img alt="Research paper thumbnail of Multitip scanning bio-Kelvin probe" class="work-thumbnail" src="https://attachments.academia-assets.com/117619555/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/123106066/Multitip_scanning_bio_Kelvin_probe">Multitip scanning bio-Kelvin probe</a></div><div class="wp-workCard_item"><span>Review of Scientific Instruments</span><span>, 1999</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">We have developed a novel multitip scanning Kelvin probe which can measure changes in biological ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">We have developed a novel multitip scanning Kelvin probe which can measure changes in biological surface potential ΔVs to within 2 mV and, quasisimultaneously monitor displacement to &lt;1 μm. The control and measurement subcomponents are PC based and incorporate a flexible user interface permitting software control of each individual tip, measurement, and scan parameters. We review the mode of operation and design features of the scanning bio-Kelvin probe including tip steering, signal processing, tip calibration, and novel tip tracking/dithering routines. This system uniquely offers both tip-to-sample spacing control (which is essential to avoid spurious changes in ΔVs due to variations in mean spacing) and a dithering routine to maintain tip orientation to the biological specimen, irrespective of the latter’s movement. These features permit long term (&gt;48 h) “active” tracking of the displacement and biopotentials developed along and around a plant shoot in response to an envir...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="34344060012bd9fdfc300e010075e045" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":117619555,"asset_id":123106066,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/117619555/download_file?st=MTczMjg0MTM3MSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="123106066"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="123106066"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 123106066; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=123106066]").text(description); $(".js-view-count[data-work-id=123106066]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 123106066; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='123106066']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 123106066, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "34344060012bd9fdfc300e010075e045" } } $('.js-work-strip[data-work-id=123106066]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":123106066,"title":"Multitip scanning bio-Kelvin probe","translated_title":"","metadata":{"abstract":"We have developed a novel multitip scanning Kelvin probe which can measure changes in biological surface potential ΔVs to within 2 mV and, quasisimultaneously monitor displacement to \u0026lt;1 μm. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="123106065"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/123106065/Spaceflight_Exposure_Effects_on_Transcription_Activity_and_Localization_of_Alcohol_Dehydrogenase_in_the_Roots_of_Arabidopsis_thaliana"><img alt="Research paper thumbnail of Spaceflight Exposure Effects on Transcription, Activity, and Localization of Alcohol Dehydrogenase in the Roots of Arabidopsis thaliana" class="work-thumbnail" src="https://attachments.academia-assets.com/117619534/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/123106065/Spaceflight_Exposure_Effects_on_Transcription_Activity_and_Localization_of_Alcohol_Dehydrogenase_in_the_Roots_of_Arabidopsis_thaliana">Spaceflight Exposure Effects on Transcription, Activity, and Localization of Alcohol Dehydrogenase in the Roots of Arabidopsis thaliana</a></div><div class="wp-workCard_item"><span>Plant Physiology</span><span>, 1997</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Although considerable research and speculation have been directed toward understanding a plant&#3...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Although considerable research and speculation have been directed toward understanding a plant&#39;s perception of gravity and the resulting gravitropic responses, little is known about the role of gravity-dependent physical processes in normal physiological function. These studies were conducted to determine whether the roots of plants exposed to spaceflight conditions may be experiencing hypoxia. Arabidopsis thaliana (L.) Heynh. plants were grown in agar medium during 6 or 11 d of spaceflight exposure on shuttle missions STS-54 (CHROMEX-03) and STS-68 (CHROMEX-05), respectively. The analysis included measurement of agar redox potential and root alcohol dehydrogenase (ADH) activity, localization, and expression. ADH activity increased by 89% as a result of spaceflight exposure for both CHROMEX-03 and -05 experiments, and ADH RNase protection assays revealed a 136% increase in ADH mRNA. The increase in ADH activity associated with the spaceflight roots was realized by a 28% decrease...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="724999e863816e33a9e50121a25efd4c" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":117619534,"asset_id":123106065,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/117619534/download_file?st=MTczMjg0MTM3MSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="123106065"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="123106065"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 123106065; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=123106065]").text(description); $(".js-view-count[data-work-id=123106065]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 123106065; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='123106065']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 123106065, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "724999e863816e33a9e50121a25efd4c" } } $('.js-work-strip[data-work-id=123106065]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":123106065,"title":"Spaceflight Exposure Effects on Transcription, Activity, and Localization of Alcohol Dehydrogenase in the Roots of Arabidopsis thaliana","translated_title":"","metadata":{"abstract":"Although considerable research and speculation have been directed toward understanding a plant\u0026#39;s perception of gravity and the resulting gravitropic responses, little is known about the role of gravity-dependent physical processes in normal physiological function. 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$a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="123106064"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/123106064/A_comparative_study_of_enzyme_immobilization_strategies_for_multi_walled_carbon_nanotube_glucose_biosensors"><img alt="Research paper thumbnail of A comparative study of enzyme immobilization strategies for multi-walled carbon nanotube glucose biosensors" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/123106064/A_comparative_study_of_enzyme_immobilization_strategies_for_multi_walled_carbon_nanotube_glucose_biosensors">A comparative study of enzyme immobilization strategies for multi-walled carbon nanotube glucose biosensors</a></div><div class="wp-workCard_item"><span>Nanotechnology</span><span>, 2011</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="123106064"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" 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wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/123106063/Oxygen_consumption_oscillates_in_single_clonal_pancreatic_beta_cells_HIT_">Oxygen consumption oscillates in single clonal pancreatic beta-cells (HIT)</a></div><div class="wp-workCard_item"><span>Diabetes</span><span>, 2000</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Based on population studies, we have hypothesized that changes in metabolism in pancreatic beta-c...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Based on population studies, we have hypothesized that changes in metabolism in pancreatic beta-cells precede changes in Ca2+. It is well known from single-cell Ca2+ studies that variable oscillatory patterns in Ca2+ occur in response to glucose stimulation. The present studies, using the clonal beta-cell line HIT-T-15, were undertaken to evaluate the relationship between glucose concentration, insulin secretion, and O2 consumption and to determine the Ca2+ dependency of glucose-induced changes in O2 consumption. In population studies, an excellent correlation was found between respiration and insulin secretion, with half-maximal values at approximately 1 mmol/l glucose for both respiration and secretion. In the absence of Ca2+, glucose stimulated O2 consumption but not insulin secretion. In single clonal beta-cells, a self-referencing O2 electrode was used to assess O2 consumption. Large-amplitude oscillations were found to occur in response to stimulation by glucose and were block...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="26c94e4b7385c6dc698b8c2ea6f8c52d" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":117619550,"asset_id":123106063,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/117619550/download_file?st=MTczMjg0MTM3MSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="123106063"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="123106063"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 123106063; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=123106063]").text(description); $(".js-view-count[data-work-id=123106063]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 123106063; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='123106063']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 123106063, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "26c94e4b7385c6dc698b8c2ea6f8c52d" } } $('.js-work-strip[data-work-id=123106063]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":123106063,"title":"Oxygen consumption oscillates in single clonal pancreatic beta-cells (HIT)","translated_title":"","metadata":{"abstract":"Based on population studies, we have hypothesized that changes in metabolism in pancreatic beta-cells precede changes in Ca2+. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> </div><div class="profile--tab_content_container js-tab-pane tab-pane" data-section-id="15818314" id="papers"><div class="js-work-strip profile--work_container" data-work-id="53418004"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/53418004/Oxygen_consumption_oscillates_in_single_clonal_pancreatic_beta_cells_HIT_"><img alt="Research paper thumbnail of Oxygen consumption oscillates in single clonal pancreatic beta-cells (HIT)" class="work-thumbnail" src="https://attachments.academia-assets.com/70271947/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/53418004/Oxygen_consumption_oscillates_in_single_clonal_pancreatic_beta_cells_HIT_">Oxygen consumption oscillates in single clonal pancreatic beta-cells (HIT)</a></div><div class="wp-workCard_item wp-workCard--coauthors"><span>by </span><span><a class="" data-click-track="profile-work-strip-authors" href="https://upenn.academia.edu/BarbaraCorkey">Barbara Corkey</a> and <a class="" data-click-track="profile-work-strip-authors" href="https://independent.academia.edu/MarshallPorterfield1">Marshall Porterfield</a></span></div><div class="wp-workCard_item"><span>Diabetes</span><span>, 2000</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="bc98e0aa662f38603b66f7f8aa00fce9" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":70271947,"asset_id":53418004,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/70271947/download_file?st=MTczMjg0MTM3MSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="53418004"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="53418004"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 53418004; 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It is well known from single-cell Ca 2+ studies that variable oscillatory patterns in Ca 2+ occur in response to glucose stimulation. The present studies, using the clonal ␤-cell line HIT-T-15, were undertaken to evaluate the relationship between glucose concentration, insulin secretion, and O 2 consumption and to determine the Ca 2+ dependency of glucose-induced changes in O 2 consumption. In population studies, an excellent correlation was found between respiration and insulin secretion, with half-maximal values at 1 mmol/l glucose for both respiration and secretion. In the absence of Ca 2+ , glucose stimulated O 2 consumption but not insulin secretion. In single clonal ␤-cells, a selfreferencing O 2 electrode was used to assess O 2 consumption. Large-amplitude oscillations were found to occur in response to stimulation by glucose and were blocked by uncoupling respiration with carbonylcyanide p-(trifluoromethoxy)phenylhydrazone (FCCP). They were also blocked and respiration totally inhibited by antimycin A, an inhibitor of complex III of the respiratory chain. Half of the cells sampled (~100 total) exhibited increased oscillatory O 2 consumption in response to glucose. Oscillations in O 2 occurred in response to glucose even in the absence of Ca 2+ , and their amplitude increased further on restoration of a normal extracellular Ca 2+ level. These studies indicated that oscillatory O 2 consumption was not dependent on Ca 2+ but that the amplitude of the O 2 oscillations increased in the presence of Ca 2+ , possibly reflecting the additional work involved in insulin secretion and Ca 2+ pumping. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="123106082"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/123106082/Aging_and_putative_frailty_biomarkers_are_altered_by_spaceflight"><img alt="Research paper thumbnail of Aging and putative frailty biomarkers are altered by spaceflight" class="work-thumbnail" src="https://attachments.academia-assets.com/117619558/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/123106082/Aging_and_putative_frailty_biomarkers_are_altered_by_spaceflight">Aging and putative frailty biomarkers are altered by spaceflight</a></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Human space exploration is hazardous, causing molecular changes that can alter astronauts&#39; he...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Human space exploration is hazardous, causing molecular changes that can alter astronauts&#39; health. This can include genomic instability, mitochondrial dysfunction, increased inflammation, homeostatic dysregulation, and epigenomic changes. These alterations are similar to changes during aging on Earth. However, little is known about the link between these changes and disease development in space. Frailty syndrome is a robust predictor associated with biological aging, however its existence during spaceflight has not been examined. We used murine data from NASA’s GeneLab and astronaut data from JAXA and Inspiration4 missions to evaluate the presence of biological markers and pathways related to frailty, aging and sarcopenia. We identified changes in gene expression that could be related to the development of a frailty-like condition. These results suggest that the parallels between spaceflight and aging may extend to frailty as well. Future studies examining the utility of a frail...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="3d24459106eb4ce0fd677dacb7f265d6" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":117619558,"asset_id":123106082,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/117619558/download_file?st=MTczMjg0MTM3MSw4LjIyMi4yMDguMTQ2&st=MTczMjg0MTM3MCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="123106082"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="123106082"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 123106082; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=123106082]").text(description); $(".js-view-count[data-work-id=123106082]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 123106082; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='123106082']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 123106082, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "3d24459106eb4ce0fd677dacb7f265d6" } } $('.js-work-strip[data-work-id=123106082]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":123106082,"title":"Aging and putative frailty biomarkers are altered by spaceflight","translated_title":"","metadata":{"abstract":"Human space exploration is hazardous, causing molecular changes that can alter astronauts\u0026#39; health. This can include genomic instability, mitochondrial dysfunction, increased inflammation, homeostatic dysregulation, and epigenomic changes. These alterations are similar to changes during aging on Earth. However, little is known about the link between these changes and disease development in space. Frailty syndrome is a robust predictor associated with biological aging, however its existence during spaceflight has not been examined. We used murine data from NASA’s GeneLab and astronaut data from JAXA and Inspiration4 missions to evaluate the presence of biological markers and pathways related to frailty, aging and sarcopenia. We identified changes in gene expression that could be related to the development of a frailty-like condition. These results suggest that the parallels between spaceflight and aging may extend to frailty as well. Future studies examining the utility of a frail...","publisher":"Research Square Platform LLC"},"translated_abstract":"Human space exploration is hazardous, causing molecular changes that can alter astronauts\u0026#39; health. This can include genomic instability, mitochondrial dysfunction, increased inflammation, homeostatic dysregulation, and epigenomic changes. These alterations are similar to changes during aging on Earth. However, little is known about the link between these changes and disease development in space. Frailty syndrome is a robust predictor associated with biological aging, however its existence during spaceflight has not been examined. We used murine data from NASA’s GeneLab and astronaut data from JAXA and Inspiration4 missions to evaluate the presence of biological markers and pathways related to frailty, aging and sarcopenia. We identified changes in gene expression that could be related to the development of a frailty-like condition. These results suggest that the parallels between spaceflight and aging may extend to frailty as well. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="123106080"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/123106080/Extraterrestrial_Gynecology_Could_Spaceflight_Increase_the_Risk_of_Developing_Cancer_in_Female_Astronauts_An_Updated_Review"><img alt="Research paper thumbnail of Extraterrestrial Gynecology: Could Spaceflight Increase the Risk of Developing Cancer in Female Astronauts? An Updated Review" class="work-thumbnail" src="https://attachments.academia-assets.com/117619538/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/123106080/Extraterrestrial_Gynecology_Could_Spaceflight_Increase_the_Risk_of_Developing_Cancer_in_Female_Astronauts_An_Updated_Review">Extraterrestrial Gynecology: Could Spaceflight Increase the Risk of Developing Cancer in Female Astronauts? An Updated Review</a></div><div class="wp-workCard_item"><span>International Journal of Molecular Sciences</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Outer space is an extremely hostile environment for human life, with ionizing radiation from gala...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Outer space is an extremely hostile environment for human life, with ionizing radiation from galactic cosmic rays and microgravity posing the most significant hazards to the health of astronauts. Spaceflight has also been shown to have an impact on established cancer hallmarks, possibly increasing carcinogenic risk. Terrestrially, women have a higher incidence of radiation-induced cancers, largely driven by lung, thyroid, breast, and ovarian cancers, and therefore, historically, they have been permitted to spend significantly less time in space than men. In the present review, we focus on the effects of microgravity and radiation on the female reproductive system, particularly gynecological cancer. The aim is to provide a summary of the research that has been carried out related to the risk of gynecological cancer, highlighting what further studies are needed to pave the way for safer exploration class missions, as well as postflight screening and management of women astronauts foll...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="fe52c86405fd16c6aeb4662283ee9285" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":117619538,"asset_id":123106080,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/117619538/download_file?st=MTczMjg0MTM3MSw4LjIyMi4yMDguMTQ2&st=MTczMjg0MTM3MCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="123106080"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="123106080"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 123106080; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=123106080]").text(description); $(".js-view-count[data-work-id=123106080]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 123106080; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='123106080']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 123106080, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "fe52c86405fd16c6aeb4662283ee9285" } } $('.js-work-strip[data-work-id=123106080]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":123106080,"title":"Extraterrestrial Gynecology: Could Spaceflight Increase the Risk of Developing Cancer in Female Astronauts? 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="123106079"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/123106079/A_Ground_based_Comparison_of_Nutrient_Delivery_Technologies_Originally_Developed_for_Growing_Plants_in_the_Spaceflight_Environment"><img alt="Research paper thumbnail of A Ground-based Comparison of Nutrient Delivery Technologies Originally Developed for Growing Plants in the Spaceflight Environment" class="work-thumbnail" src="https://attachments.academia-assets.com/117619539/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/123106079/A_Ground_based_Comparison_of_Nutrient_Delivery_Technologies_Originally_Developed_for_Growing_Plants_in_the_Spaceflight_Environment">A Ground-based Comparison of Nutrient Delivery Technologies Originally Developed for Growing Plants in the Spaceflight Environment</a></div><div class="wp-workCard_item"><span>HortTechnology</span><span>, 2000</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">A ground-based comparison of plant nutrient delivery systems that have been developed for microgr...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">A ground-based comparison of plant nutrient delivery systems that have been developed for microgravity application was conducted for dwarf wheat (Triticum aestivum L. `Yecora Rojo&#39;) and rapid-cycling brassica (Brassica rapa L. CrGC#1-33) plants. These experiments offer insight into nutrient and oxygen delivery concerns for greenhouse crop production systems. The experiments were completed over a 12-day period to simulate a typical space shuttle-based spaceflight experiment. The plant materials, grown either using the porous-tube nutrient delivery system, the phenolic foam support system, or a solidified agar nutrient medium, were compared by plant-growth analysis, root zone morphological measurements, elemental composition analysis, and alcohol dehydrogenase enzyme activity assay. The results of these analyses indicate that the porous tube plant nutrient delivery and the phenolic foam systems maintain plant growth at a higher level than the solidified agar gel medium system. Roo...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="2616932da329ae4984b8e0243e91cd86" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":117619539,"asset_id":123106079,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/117619539/download_file?st=MTczMjg0MTM3MSw4LjIyMi4yMDguMTQ2&st=MTczMjg0MTM3MCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="123106079"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="123106079"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 123106079; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=123106079]").text(description); $(".js-view-count[data-work-id=123106079]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 123106079; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='123106079']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 123106079, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "2616932da329ae4984b8e0243e91cd86" } } $('.js-work-strip[data-work-id=123106079]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":123106079,"title":"A Ground-based Comparison of Nutrient Delivery Technologies Originally Developed for Growing Plants in the Spaceflight Environment","translated_title":"","metadata":{"abstract":"A ground-based comparison of plant nutrient delivery systems that have been developed for microgravity application was conducted for dwarf wheat (Triticum aestivum L. `Yecora Rojo\u0026#39;) and rapid-cycling brassica (Brassica rapa L. 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class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/123106078/Proteins_and_lipids_define_the_diffusional_field_of_nitric_oxide">Proteins and lipids define the diffusional field of nitric oxide</a></div><div class="wp-workCard_item"><span>American Journal of Physiology-Lung Cellular and Molecular Physiology</span><span>, 2001</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Nitric oxide (NO) fluxes released from the surface of individual activated macrophages or cells l...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Nitric oxide (NO) fluxes released from the surface of individual activated macrophages or cells localized in small aggregates were measured with a novel polarographic self-referencing microsensor. NO fluxes could be detected at distances from the cells of 100–500 μm. The initial flux and the distance from the cells at which NO could be detected were directly related to the number of cells in the immediate vicinity of the probe releasing NO. Thus, whereas NO fluxes of ∼1 pmol · cm−2· s−1were measured from individual macrophages, aggregates composed of groups of cells varying in number from 18 to 48 cells produced NO fluxes of between ∼4 and 10 pmol · cm−2· s−1. NO fluxes required the presence of l-arginine. Signals were significantly reduced by the addition of hemoglobin and by N-nitro-l-arginine methyl ester. NO fluxes were greatest when the sensor was placed immediately adjacent to cell membranes and declined as the distance from the cell increased. The NO signal was markedly reduc...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="7c003b7e907abdf530a118a403c78c7a" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":117619536,"asset_id":123106078,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/117619536/download_file?st=MTczMjg0MTM3MSw4LjIyMi4yMDguMTQ2&st=MTczMjg0MTM3MCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="123106078"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="123106078"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 123106078; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=123106078]").text(description); $(".js-view-count[data-work-id=123106078]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 123106078; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='123106078']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 123106078, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "7c003b7e907abdf530a118a403c78c7a" } } $('.js-work-strip[data-work-id=123106078]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":123106078,"title":"Proteins and lipids define the diffusional field of nitric oxide","translated_title":"","metadata":{"abstract":"Nitric oxide (NO) fluxes released from the surface of individual activated macrophages or cells localized in small aggregates were measured with a novel polarographic self-referencing microsensor. 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fern spores</a></div><div class="wp-workCard_item"><span>Lab on a Chip</span><span>, 2017</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">A device designed for space flight measures the longest continuous observation of a plant cell&#3...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">A device designed for space flight measures the longest continuous observation of a plant cell&#39;s calcium response to hypergravity made to date.</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper 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Non-invasive detection of ascorbate fluxes in indivdual, oxidatively-stressed early embryos" class="work-thumbnail" src="https://attachments.academia-assets.com/117619556/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/123106075/P_157_Non_invasive_detection_of_ascorbate_fluxes_in_indivdual_oxidatively_stressed_early_embryos">P-157. Non-invasive detection of ascorbate fluxes in indivdual, oxidatively-stressed early embryos</a></div><div class="wp-workCard_item"><span>Human Reproduction</span><span>, 1999</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="91cf5531c006b4f43391c396250293a6" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":117619556,"asset_id":123106075,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/117619556/download_file?st=MTczMjg0MTM3MSw4LjIyMi4yMDguMTQ2&st=MTczMjg0MTM3MCw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="123106075"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="123106075"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 123106075; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=123106075]").text(description); $(".js-view-count[data-work-id=123106075]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 123106075; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='123106075']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 123106075, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "91cf5531c006b4f43391c396250293a6" } } $('.js-work-strip[data-work-id=123106075]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":123106075,"title":"P-157. 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class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/123106066/Multitip_scanning_bio_Kelvin_probe"><img alt="Research paper thumbnail of Multitip scanning bio-Kelvin probe" class="work-thumbnail" src="https://attachments.academia-assets.com/117619555/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/123106066/Multitip_scanning_bio_Kelvin_probe">Multitip scanning bio-Kelvin probe</a></div><div class="wp-workCard_item"><span>Review of Scientific Instruments</span><span>, 1999</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">We have developed a novel multitip scanning Kelvin probe which can measure changes in biological ...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">We have developed a novel multitip scanning Kelvin probe which can measure changes in biological surface potential ΔVs to within 2 mV and, quasisimultaneously monitor displacement to &lt;1 μm. The control and measurement subcomponents are PC based and incorporate a flexible user interface permitting software control of each individual tip, measurement, and scan parameters. We review the mode of operation and design features of the scanning bio-Kelvin probe including tip steering, signal processing, tip calibration, and novel tip tracking/dithering routines. This system uniquely offers both tip-to-sample spacing control (which is essential to avoid spurious changes in ΔVs due to variations in mean spacing) and a dithering routine to maintain tip orientation to the biological specimen, irrespective of the latter’s movement. These features permit long term (&gt;48 h) “active” tracking of the displacement and biopotentials developed along and around a plant shoot in response to an envir...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="34344060012bd9fdfc300e010075e045" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":117619555,"asset_id":123106066,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/117619555/download_file?st=MTczMjg0MTM3MSw4LjIyMi4yMDguMTQ2&st=MTczMjg0MTM3MSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="123106066"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="123106066"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 123106066; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=123106066]").text(description); $(".js-view-count[data-work-id=123106066]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 123106066; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='123106066']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 123106066, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "34344060012bd9fdfc300e010075e045" } } $('.js-work-strip[data-work-id=123106066]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":123106066,"title":"Multitip scanning bio-Kelvin probe","translated_title":"","metadata":{"abstract":"We have developed a novel multitip scanning Kelvin probe which can measure changes in biological surface potential ΔVs to within 2 mV and, quasisimultaneously monitor displacement to \u0026lt;1 μm. 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$(this).data('initialized', true); } }); $a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="123106065"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/123106065/Spaceflight_Exposure_Effects_on_Transcription_Activity_and_Localization_of_Alcohol_Dehydrogenase_in_the_Roots_of_Arabidopsis_thaliana"><img alt="Research paper thumbnail of Spaceflight Exposure Effects on Transcription, Activity, and Localization of Alcohol Dehydrogenase in the Roots of Arabidopsis thaliana" class="work-thumbnail" src="https://attachments.academia-assets.com/117619534/thumbnails/1.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/123106065/Spaceflight_Exposure_Effects_on_Transcription_Activity_and_Localization_of_Alcohol_Dehydrogenase_in_the_Roots_of_Arabidopsis_thaliana">Spaceflight Exposure Effects on Transcription, Activity, and Localization of Alcohol Dehydrogenase in the Roots of Arabidopsis thaliana</a></div><div class="wp-workCard_item"><span>Plant Physiology</span><span>, 1997</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Although considerable research and speculation have been directed toward understanding a plant&#3...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Although considerable research and speculation have been directed toward understanding a plant&#39;s perception of gravity and the resulting gravitropic responses, little is known about the role of gravity-dependent physical processes in normal physiological function. These studies were conducted to determine whether the roots of plants exposed to spaceflight conditions may be experiencing hypoxia. Arabidopsis thaliana (L.) Heynh. plants were grown in agar medium during 6 or 11 d of spaceflight exposure on shuttle missions STS-54 (CHROMEX-03) and STS-68 (CHROMEX-05), respectively. The analysis included measurement of agar redox potential and root alcohol dehydrogenase (ADH) activity, localization, and expression. ADH activity increased by 89% as a result of spaceflight exposure for both CHROMEX-03 and -05 experiments, and ADH RNase protection assays revealed a 136% increase in ADH mRNA. The increase in ADH activity associated with the spaceflight roots was realized by a 28% decrease...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="724999e863816e33a9e50121a25efd4c" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":117619534,"asset_id":123106065,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/117619534/download_file?st=MTczMjg0MTM3MSw4LjIyMi4yMDguMTQ2&st=MTczMjg0MTM3MSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="123106065"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="123106065"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 123106065; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=123106065]").text(description); $(".js-view-count[data-work-id=123106065]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 123106065; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='123106065']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 123106065, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "724999e863816e33a9e50121a25efd4c" } } $('.js-work-strip[data-work-id=123106065]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":123106065,"title":"Spaceflight Exposure Effects on Transcription, Activity, and Localization of Alcohol Dehydrogenase in the Roots of Arabidopsis thaliana","translated_title":"","metadata":{"abstract":"Although considerable research and speculation have been directed toward understanding a plant\u0026#39;s perception of gravity and the resulting gravitropic responses, little is known about the role of gravity-dependent physical processes in normal physiological function. 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$a.trackClickSource(".js-work-strip-work-link", "profile_work_strip") }); </script> <div class="js-work-strip profile--work_container" data-work-id="123106064"><div class="profile--work_thumbnail hidden-xs"><a class="js-work-strip-work-link" data-click-track="profile-work-strip-thumbnail" href="https://www.academia.edu/123106064/A_comparative_study_of_enzyme_immobilization_strategies_for_multi_walled_carbon_nanotube_glucose_biosensors"><img alt="Research paper thumbnail of A comparative study of enzyme immobilization strategies for multi-walled carbon nanotube glucose biosensors" class="work-thumbnail" src="https://a.academia-assets.com/images/blank-paper.jpg" /></a></div><div class="wp-workCard wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/123106064/A_comparative_study_of_enzyme_immobilization_strategies_for_multi_walled_carbon_nanotube_glucose_biosensors">A comparative study of enzyme immobilization strategies for multi-walled carbon nanotube glucose biosensors</a></div><div class="wp-workCard_item"><span>Nanotechnology</span><span>, 2011</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="123106064"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" 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wp-workCard_itemContainer"><div class="wp-workCard_item wp-workCard--title"><a class="js-work-strip-work-link text-gray-darker" data-click-track="profile-work-strip-title" href="https://www.academia.edu/123106063/Oxygen_consumption_oscillates_in_single_clonal_pancreatic_beta_cells_HIT_">Oxygen consumption oscillates in single clonal pancreatic beta-cells (HIT)</a></div><div class="wp-workCard_item"><span>Diabetes</span><span>, 2000</span></div><div class="wp-workCard_item"><span class="js-work-more-abstract-truncated">Based on population studies, we have hypothesized that changes in metabolism in pancreatic beta-c...</span><a class="js-work-more-abstract" data-broccoli-component="work_strip.more_abstract" data-click-track="profile-work-strip-more-abstract" href="javascript:;"><span> more </span><span><i class="fa fa-caret-down"></i></span></a><span class="js-work-more-abstract-untruncated hidden">Based on population studies, we have hypothesized that changes in metabolism in pancreatic beta-cells precede changes in Ca2+. It is well known from single-cell Ca2+ studies that variable oscillatory patterns in Ca2+ occur in response to glucose stimulation. The present studies, using the clonal beta-cell line HIT-T-15, were undertaken to evaluate the relationship between glucose concentration, insulin secretion, and O2 consumption and to determine the Ca2+ dependency of glucose-induced changes in O2 consumption. In population studies, an excellent correlation was found between respiration and insulin secretion, with half-maximal values at approximately 1 mmol/l glucose for both respiration and secretion. In the absence of Ca2+, glucose stimulated O2 consumption but not insulin secretion. In single clonal beta-cells, a self-referencing O2 electrode was used to assess O2 consumption. Large-amplitude oscillations were found to occur in response to stimulation by glucose and were block...</span></div><div class="wp-workCard_item wp-workCard--actions"><span class="work-strip-bookmark-button-container"></span><a id="26c94e4b7385c6dc698b8c2ea6f8c52d" class="wp-workCard--action" rel="nofollow" data-click-track="profile-work-strip-download" data-download="{"attachment_id":117619550,"asset_id":123106063,"asset_type":"Work","button_location":"profile"}" href="https://www.academia.edu/attachments/117619550/download_file?st=MTczMjg0MTM3MSw4LjIyMi4yMDguMTQ2&st=MTczMjg0MTM3MSw4LjIyMi4yMDguMTQ2&s=profile"><span><i class="fa fa-arrow-down"></i></span><span>Download</span></a><span class="wp-workCard--action visible-if-viewed-by-owner inline-block" style="display: none;"><span class="js-profile-work-strip-edit-button-wrapper profile-work-strip-edit-button-wrapper" data-work-id="123106063"><a class="js-profile-work-strip-edit-button" tabindex="0"><span><i class="fa fa-pencil"></i></span><span>Edit</span></a></span></span><span id="work-strip-rankings-button-container"></span></div><div class="wp-workCard_item wp-workCard--stats"><span><span><span class="js-view-count view-count u-mr2x" data-work-id="123106063"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 123106063; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=123106063]").text(description); $(".js-view-count[data-work-id=123106063]").attr('title', description).tooltip(); }); });</script></span></span><span><span class="percentile-widget hidden"><span class="u-mr2x work-percentile"></span></span><script>$(function () { var workId = 123106063; window.Academia.workPercentilesFetcher.queue(workId, function (percentileText) { var container = $(".js-work-strip[data-work-id='123106063']"); container.find('.work-percentile').text(percentileText.charAt(0).toUpperCase() + percentileText.slice(1)); container.find('.percentile-widget').show(); container.find('.percentile-widget').removeClass('hidden'); }); });</script></span><span><script>$(function() { new Works.PaperRankView({ workId: 123106063, container: "", }); });</script></span></div><div id="work-strip-premium-row-container"></div></div></div><script> require.config({ waitSeconds: 90 })(["https://a.academia-assets.com/assets/wow_profile-f77ea15d77ce96025a6048a514272ad8becbad23c641fc2b3bd6e24ca6ff1932.js","https://a.academia-assets.com/assets/work_edit-ad038b8c047c1a8d4fa01b402d530ff93c45fee2137a149a4a5398bc8ad67560.js"], function() { // from javascript_helper.rb var dispatcherData = {} if (true){ window.WowProfile.dispatcher = window.WowProfile.dispatcher || _.clone(Backbone.Events); dispatcherData = { dispatcher: window.WowProfile.dispatcher, downloadLinkId: "26c94e4b7385c6dc698b8c2ea6f8c52d" } } $('.js-work-strip[data-work-id=123106063]').each(function() { if (!$(this).data('initialized')) { new WowProfile.WorkStripView({ el: this, workJSON: {"id":123106063,"title":"Oxygen consumption oscillates in single clonal pancreatic beta-cells (HIT)","translated_title":"","metadata":{"abstract":"Based on population studies, we have hypothesized that changes in metabolism in pancreatic beta-cells precede changes in Ca2+. It is well known from single-cell Ca2+ studies that variable oscillatory patterns in Ca2+ occur in response to glucose stimulation. The present studies, using the clonal beta-cell line HIT-T-15, were undertaken to evaluate the relationship between glucose concentration, insulin secretion, and O2 consumption and to determine the Ca2+ dependency of glucose-induced changes in O2 consumption. In population studies, an excellent correlation was found between respiration and insulin secretion, with half-maximal values at approximately 1 mmol/l glucose for both respiration and secretion. In the absence of Ca2+, glucose stimulated O2 consumption but not insulin secretion. In single clonal beta-cells, a self-referencing O2 electrode was used to assess O2 consumption. Large-amplitude oscillations were found to occur in response to stimulation by glucose and were block...","publisher":"American Diabetes Association","publication_date":{"day":null,"month":null,"year":2000,"errors":{}},"publication_name":"Diabetes"},"translated_abstract":"Based on population studies, we have hypothesized that changes in metabolism in pancreatic beta-cells precede changes in Ca2+. It is well known from single-cell Ca2+ studies that variable oscillatory patterns in Ca2+ occur in response to glucose stimulation. The present studies, using the clonal beta-cell line HIT-T-15, were undertaken to evaluate the relationship between glucose concentration, insulin secretion, and O2 consumption and to determine the Ca2+ dependency of glucose-induced changes in O2 consumption. In population studies, an excellent correlation was found between respiration and insulin secretion, with half-maximal values at approximately 1 mmol/l glucose for both respiration and secretion. In the absence of Ca2+, glucose stimulated O2 consumption but not insulin secretion. In single clonal beta-cells, a self-referencing O2 electrode was used to assess O2 consumption. Large-amplitude oscillations were found to occur in response to stimulation by glucose and were block...","internal_url":"https://www.academia.edu/123106063/Oxygen_consumption_oscillates_in_single_clonal_pancreatic_beta_cells_HIT_","translated_internal_url":"","created_at":"2024-08-21T21:08:57.527-07:00","preview_url":null,"current_user_can_edit":null,"current_user_is_owner":null,"owner_id":231658143,"coauthors_can_edit":true,"document_type":"paper","co_author_tags":[],"downloadable_attachments":[{"id":117619550,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/117619550/thumbnails/1.jpg","file_name":"10969835.pdf","download_url":"https://www.academia.edu/attachments/117619550/download_file?st=MTczMjg0MTM3MSw4LjIyMi4yMDguMTQ2&st=MTczMjg0MTM3MSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Oxygen_consumption_oscillates_in_single.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/117619550/10969835-libre.pdf?1724302321=\u0026response-content-disposition=attachment%3B+filename%3DOxygen_consumption_oscillates_in_single.pdf\u0026Expires=1732844971\u0026Signature=fFUCMnMYGZGhEQIZ9wm64syNIIeoMwupoaLTU8pF7TNlfVoyPOHgEqtJIiXQ59Q7h2ENty-VoT3HRS5j~k3b~P1VEfG7BeM9JcoANxQLmsaWgHGUxhp9pxqQOL1cTLcaSl3qdg9Zs~iFKIrnSaNvoUU6IuWLO0Em-B3b3kcUZLx7hsJMPTjoqDdycDDB1ylAlwDS3RobAk~NlEDUqxDWda0FDth1maKvNMVFEE-wNB5DQEo1E7OTOwVSjRAQtTfJokIpajPdwEO8cpRESU1I-JSA0rBt9xHD-TJdN0uvX-lmVI0y0W9D-uSTntk45-~vSbmjrOf9spJFKgNy0qawjA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"slug":"Oxygen_consumption_oscillates_in_single_clonal_pancreatic_beta_cells_HIT_","translated_slug":"","page_count":6,"language":"en","content_type":"Work","owner":{"id":231658143,"first_name":"Marshall","middle_initials":null,"last_name":"Porterfield","page_name":"MarshallPorterfield1","domain_name":"independent","created_at":"2022-08-11T06:19:49.618-07:00","display_name":"Marshall Porterfield","url":"https://independent.academia.edu/MarshallPorterfield1"},"attachments":[{"id":117619550,"title":"","file_type":"pdf","scribd_thumbnail_url":"https://attachments.academia-assets.com/117619550/thumbnails/1.jpg","file_name":"10969835.pdf","download_url":"https://www.academia.edu/attachments/117619550/download_file?st=MTczMjg0MTM3MSw4LjIyMi4yMDguMTQ2&st=MTczMjg0MTM3MSw4LjIyMi4yMDguMTQ2&","bulk_download_file_name":"Oxygen_consumption_oscillates_in_single.pdf","bulk_download_url":"https://d1wqtxts1xzle7.cloudfront.net/117619550/10969835-libre.pdf?1724302321=\u0026response-content-disposition=attachment%3B+filename%3DOxygen_consumption_oscillates_in_single.pdf\u0026Expires=1732844971\u0026Signature=fFUCMnMYGZGhEQIZ9wm64syNIIeoMwupoaLTU8pF7TNlfVoyPOHgEqtJIiXQ59Q7h2ENty-VoT3HRS5j~k3b~P1VEfG7BeM9JcoANxQLmsaWgHGUxhp9pxqQOL1cTLcaSl3qdg9Zs~iFKIrnSaNvoUU6IuWLO0Em-B3b3kcUZLx7hsJMPTjoqDdycDDB1ylAlwDS3RobAk~NlEDUqxDWda0FDth1maKvNMVFEE-wNB5DQEo1E7OTOwVSjRAQtTfJokIpajPdwEO8cpRESU1I-JSA0rBt9xHD-TJdN0uvX-lmVI0y0W9D-uSTntk45-~vSbmjrOf9spJFKgNy0qawjA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA"}],"research_interests":[{"id":154,"name":"Endocrinology","url":"https://www.academia.edu/Documents/in/Endocrinology"},{"id":4581,"name":"Diabetes","url":"https://www.academia.edu/Documents/in/Diabetes"},{"id":4987,"name":"Kinetics","url":"https://www.academia.edu/Documents/in/Kinetics"},{"id":7710,"name":"Biology","url":"https://www.academia.edu/Documents/in/Biology"},{"id":9534,"name":"Calcium","url":"https://www.academia.edu/Documents/in/Calcium"},{"id":26327,"name":"Medicine","url":"https://www.academia.edu/Documents/in/Medicine"},{"id":64336,"name":"Population","url":"https://www.academia.edu/Documents/in/Population"},{"id":65390,"name":"Internal Medicine","url":"https://www.academia.edu/Documents/in/Internal_Medicine"},{"id":71289,"name":"Glucose","url":"https://www.academia.edu/Documents/in/Glucose"},{"id":71400,"name":"Insulin","url":"https://www.academia.edu/Documents/in/Insulin"},{"id":174781,"name":"Oscillations","url":"https://www.academia.edu/Documents/in/Oscillations"},{"id":202428,"name":"Respiration","url":"https://www.academia.edu/Documents/in/Respiration"},{"id":541937,"name":"Stimulation","url":"https://www.academia.edu/Documents/in/Stimulation"},{"id":1193624,"name":"Oxygen Consumption","url":"https://www.academia.edu/Documents/in/Oxygen_Consumption"},{"id":1274621,"name":"Extracellular","url":"https://www.academia.edu/Documents/in/Extracellular"},{"id":1705044,"name":"islets of Langerhans","url":"https://www.academia.edu/Documents/in/islets_of_Langerhans"},{"id":3763225,"name":"Medical and Health Sciences","url":"https://www.academia.edu/Documents/in/Medical_and_Health_Sciences"},{"id":3950591,"name":"Antimycin A","url":"https://www.academia.edu/Documents/in/Antimycin_A"}],"urls":[{"id":44163786,"url":"https://journals.org/diabetes/diabetes/article-pdf/49/9/1511/365225/10969835.pdf"}]}, dispatcherData: dispatcherData }); 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