Solid-Phase Strategy based Library Screening Service

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We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects. </p> <b class="Highlights_tit">Highlights</b> <ul class="HighlightsArea"> <li class="col-md-6 col-lg-6 col-sm-6"> <img src="static/images/Abouts-menu-lights-pic-1.jpg" srcset="static/images/Abouts-menu-lights-pic-1.webp 1x" width="200" height="131" loading="lazy"><a href="aboutus.html" class="bannerbutton"> <i class="fa fa-angle-right"></i>About CBL </a> </li> <li class="col-md-6 col-lg-6 col-sm-6"> <img src="static/images/Abouts-menu-lights-pic-2.jpg" srcset="static/images/Abouts-menu-lights-pic-2.webp 1x" width="200" height="131" loading="lazy"><a href="contactus.html" class="bannerbutton"> <i class="fa fa-angle-right"></i>Contact Us </a> </li> </ul> </li> </ul> </div> </div> </li> </ul> <script> window.addEventListener('load',function(){ var arr = ['/drug-discovery/therapeutics/','/bioconjugation/','/adc/']; $('.mennav ul li a').each(function(){ var $this = $(this); var href = $this.attr("href").toLocaleLowerCase(); $.each(arr, function(index, value) { if (href && href.startsWith(value)) { $this.attr('target', '_blank'); } }); }); }); </script> </div> <div class="top-search search-group"> <div class="col-md-5 col-sm-6"> <label class="radio-inline"><input type="radio" name="search" class="rdo_productsearch" value="product" data-type="product" placeholder="Please input cat#, product name, abbr, or keywords."> Product</label> <label class="radio-inline"><input type="radio" name="search" class="rdo_servicesearch" value="service" data-type="service" placeholder="Please input your keywords."> Service</label> <label class="radio-inline"><input type="radio" name="search" class="rdo_googlesearch" value="google" data-type="google" placeholder="Please input your keywords." checked="checked"> Keyword</label> </div> <div class="col-md-7 col-sm-6 search-div"> <input id="keys" name="keys" placeholder="Please input your keywords." class="search-input"> <div class="selectItems"></div> <span class="search_btn"><i class="fa fa-search"></i></span> </div> </div> </div> <div class="searchShow"> <div class="searchtopbg"></div> <span class="searchclosed"><i class="fa fa-times"></i>Close</span> <div class="addsearch search-group"></div> </div> <div class="banner_inpage services_bg_2"> <div class="auto-container small_content-width about_container"> <ul class="bread-crumb clearfix"> <li><a href="/">Home</a></li> <li><a href="javascrpt:void(0)">Discovery</a></li><li><a href="javascrpt:void(0)">By Technology</a></li><li><a href="/phage-display-service.html">Phage Display based Binder Discovery</a></li><li><a href="/phage-display-library-screening.html">Phage Display Library Screening</a></li> <li>Solid-Phase Screening</li> </ul> <p>"Creative Biolabs is committed to providing highly customized comprehensive solutions with the best quality to advance our global clients’ projects."</p> </div> </div> <script> window.addEventListener('load', function () { if (($('.bread-crumb li:last-child').text()) == "Peer-Reviewed Publications") { $(".banner_inpage").addClass("peer-reviewed-publications_bg").removeClass("services_bg_2"); } if ($(window).hasClass('bread-crumb')) { if ($(".bread-crumb").html().indexOf("RESOURCE") > -1) { $(".pronote").hide(); } if ($(".bread-crumb").html().indexOf("Careers") > -1) { $(".pronote").hide(); } } }); </script> <div class="small_content-width services-container"> <div class="col-md-9"> <div class="inpage_section_title"> <h1>Solid-Phase Strategy based Library Screening Service</h1> </div> <div class="page-tab"> <span onclick="window.location.href=window.location.pathname+'#tab1'">Background</span> <span onclick="window.location.href=window.location.pathname+'#tab2'">Service</span> <span onclick="window.location.href=window.location.pathname+'#tab3'">FAQ</span> <span onclick="window.location.href=window.location.pathname+'#tab4'">Resources</span> </div> <script>window.addEventListener('load',function(){page_tab_margin();page_tab_css();})</script> <p> <strong>Creative Biolabs</strong> is a well-recognized service provider of phage display technology. We have developed a serial of advanced<a href="/phage-display-library-screening.html"> phage display library screening</a> methods for selecting antibodies with high affinity and specificity. Solid-phase screening is a valuable method that is rapid, efficient and relatively inexpensive for selecting the binding antibodies, identifying epitopes, etc. We offer an optimized solid-phase screening strategy which guarantees the success of finding binders targeting various types of antigens. </p> <h2 class="h2_tit page_tab_margin" id="tab1"> Background </h2> <p> Phage display is one of the most effective technology for antibody generation since it links the phage phenotype and its encapsulated genotype. In general, the peptide or antibody library is constructed by fusing interested proteins with pIII, pVIII or other capsid proteins and displaying on the surface of the M13 phage particle. Our seasoned scientists are confident in generating high-quality libraries with diversity over 10<sup>10</sup>. </p> <p> Phage display library has become a popular tool for treating autoimmune and cancer diseases. While the biopanning stage plays a crucial role in the enrichment and isolation of desired binders that can effectively recognize the interested targets. This stage involves a repeated cycles of incubation, washing, elution and amplification. Multiple rounds of selection cycle are required for obtaining monoclonal phage antibodies with desired binding affinity. Enzyme-linked immunosorbent assay (ELISA) could also be incorporated to determine the activity after the selection. As the most widely used biopanning method, solid-phase screening indicates an effective selection approach that can apply to most of binder selection projects. A series of solid-phase, like microtiter plate wells, PVDF membranes, could be used for target molecule immobilization. </p> <p class="ServiceShowPic"> <img src="static/img/Process-of-phage-display-library-selection.png" width="388" alt="Phage display library selection. (Creative Biolabs Original)" height="354" loading="lazy" />Fig.1 Process of phage display library selection. </p> <p> Several components in the screening system affect the selection result, such as the type of solid support, time of binding, washing and elution, and antigen concentration. We have developed an optimized procedure which minimizes the possibility of selecting target-unrelated binders. In order to reduce the binding to plastic, several methods could be applied, such as blocking the surface or immobilizing the target using an alternative approach other than direct adsorption. The density of the target could be increased to reduce the possibility of selecting background binders. Also, subtractive selection could be used to remove background binders. </p> <h2 class="h2_tit page_tab_margin" id="tab2"> Solid-Phase Strategy based Library Screening Service </h2> <p> <strong>Creative Biolabs</strong> is highly experienced in phage display technology and has proven records of generating high-affinity antibodies in a cost-effective and timely manner. Our solid-phase screening is optimized and precisely controlled, therefore avoiding the possibility of false-positives. Combined with other advanced technologies, we could offer the most comprehensive services and products based on our phage display platform, from immunization, library construction and screening to antibody engineering. Our scientists are pleased to offer tailored solutions to meet our customers' different projects. </p> <p> Other optional <a href="/phage-display-library-screening.html">phage display library screening</a> services: </p> <ul class="ullist"> <li> <a href="/In-vivo-Phage-Display-Service.htm"><em>In Vivo</em> Phage Library Screening Services</a> </li> <li> <a href="/Ex-vivo-Phage-Display-Services.htm"><em>Ex vivo</em> Phage Display Services</a> </li> <li> <a href="/Magic-Therapeutic-Antibody-Discovery-Service.htm">Magic™ Therapeutic Antibody Discovery Service</a> </li> <li> <a href="/solid-phase-library-screening.html">Solid-phase Library Screening</a> </li> <li> <a href="/solution-sorting-library-screening.html">Solution-sorting Library Screening</a> </li> <li> <a href="/cell-based-library-screening.html">Cell-based Library Screening</a> </li> <li> <a href="/Premade-Phage-Display-Antibody-Library-Screening-Service.html">Premade Phage Display Antibody Library Screening Service</a> </li> <li> <a href="/Premade-Phage-Display-Peptide-Library-Screening-Service.html">Premade Phage Display Peptide Library Screening Service</a> </li> <li> <a href="/rapid-recovery-of-scfv.html">Rapid Recovery of scFv</a> </li> </ul> <script type="text/javascript"> window.addEventListener('load',function(){ faqlist(4); }); </script> <h2 class="h2_tit page_tab_margin" id="tab3"> FAQ </h2> <ol class="ollist faqlist"> <li> <span class="sFAQTit">What is solid-phase phage display library screening</span> <p> Solid-phase phage display library screening is a technique used to identify peptides, proteins, or antibodies that bind to a specific target immobilized on a solid surface. Phages displaying various peptides are introduced to the target, and those that bind are retained, while non-binders are washed away. The process identifies high-affinity binding molecules that can be further studied or developed into therapeutic agents. </p> </li> <li> <span class="sFAQTit">How does solid-phase phage display screening work</span> <p> In solid-phase phage display screening, the target molecule is immobilized on a solid surface such as a microplate or bead. A phage display library containing millions of phages with different peptide sequences is incubated with the target. Phages that bind to the target remain on the surface, while unbound phages are washed away. Bound phages are then eluted and amplified for further rounds of selection. </p> </li> <li> <span class="sFAQTit">What are the advantages of using solid-phase phage display over other methods</span> <p> Solid-phase phage display offers high throughput and sensitivity, allowing for the screening of large peptide libraries against immobilized targets. The solid-phase format simplifies washing steps and ensures that only high-affinity binders are retained. Additionally, this method allows for the easy modification of the solid surface to accommodate different targets, making it versatile for various applications. </p> </li> <li> <span class="sFAQTit">What are the key applications of solid-phase phage display screening</span> <p> Solid-phase phage display is widely used in drug discovery, antibody development, and the identification of protein-protein interactions. It helps researchers find peptides or antibodies that bind to disease-related targets, enabling the development of new therapies. This method is also employed in diagnostics, vaccine development, and the creation of biopharmaceuticals by identifying high-affinity binding molecules. </p> </li> <li> <span class="sFAQTit">What types of targets can be used in solid-phase phage display screening</span> <p> Various types of targets can be used in solid-phase phage display screening, including proteins, peptides, nucleic acids, and small molecules. The target is typically immobilized on a solid surface, and the phage display library is screened against it to identify binding interactions. This versatility makes solid-phase phage display applicable across multiple fields, including immunology, cancer research, and infectious disease. </p> </li> <li> <span class="sFAQTit">How does solid-phase phage display differ from solution-phase screening</span> <p> In solid-phase screening, the target is immobilized on a surface, whereas, in solution-phase screening, the target remains in a liquid medium. Solid-phase screening allows for easier separation of bound and unbound phages, reducing background noise and enabling more efficient identification of high-affinity binders. Solution-phase screening, on the other hand, may offer more natural binding conditions, but with increased complexity in washing and separation steps. </p> </li> <li> <span class="sFAQTit">What are the typical steps involved in solid-phase phage display screening</span> <p> The typical steps include immobilizing the target on a solid surface, incubating the phage display library with the target, washing away unbound phages, and eluting bound phages. The bound phages are then amplified and subjected to additional rounds of selection to enrich for high-affinity binders. After multiple rounds, the DNA of the selected phages is sequenced to identify the binding peptides. </p> </li> <li> <span class="sFAQTit">How is solid-phase phage display used in antibody discovery</span> <p> Solid-phase phage display is a critical technique in antibody discovery, enabling the identification of antibodies that bind to specific antigens immobilized on a surface. By screening large antibody libraries, this method allows for the selection of high-affinity and specific antibodies, which can then be further developed as diagnostic tools, therapeutic agents, or research reagents. </p> </li> </ol> <h2 class="h2_tit page_tab_margin" id="tab4"> Resources </h2> <p> Use the resources in our library to help you understand your options and make critical decisions for your study. </p> <div class="tab_nav"> <span class="tab_choosebg">Videos</span> <span>Podcasts</span> <span>Infographic</span> <span>Flyer</span> <span>Articles</span> </div>  <div class="tab_cont" style="display:block;"> <div class="row pagerow_pt"> <div class="col-md-4"> <div class="pagerow_pt_unit"> <img loading="lazy" src="../static/img/video-phage-display-technology.png" alt="" /> </div> <a href="https://youtu.be/_Cqj5gphNug" target="_blank">Antibody based Cancer Therapies </a> </div> </div> </div>  <div class="tab_cont"> <div class="therapy_ad"> <a href="therapeutic-antibody.html"> <img loading="lazy" src="../static/img/podcasts-ad.png" width="1600" height="300" srcset="../static/img/podcasts-ad.webp 1x,../static/img/podcasts-ad.webp 2x,../static/img/podcasts-ad.webp 3x" alt="In this brand new podcast series, we will show you everything you want to know about CAR-T therapy, including the mechanism, current applications, technology limitations, and potential strategies." /></a> </div> </div>  <div class="tab_cont"> <div class="row pagerow_pt"> <div class="col-md-4"> <div class="pagerow_pt_unit_2"> <img loading="lazy" src="static/img/antibody-based-therapies-creative-biolabs-11.jpg" width="600" height="500" alt="" /> </div> <a href="https://go.creative-biolabs.com/antibody-based-therapies.html">Antibody-Based Therapies</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit_2"> <img loading="lazy" src="static/img/Development-of-Monoclonal-Antibodies-1.jpg" width="600" height="500" alt="" /> </div> <a href="https://go.creative-biolabs.com/development-of-monoclonal-antibodies.html">Development of Monoclonal Antibodies</a> </div> </div> </div>  <div class="tab_cont"> <div class="row pagerow_pt"> <div class="col-md-4"> <div class="pagerow_pt_unit_2"> <img loading="lazy" src="/resource/images/Phage-Display-Technology-Flyer-Creative-Biolabs.jpg" width="600" height="500" alt="" /> </div> <a href="/resource/pdf/com/flyers/Phage-Display-Technology-Flyer-Creative-Biolabs.pdf">Phage Display Technology in Creative Biolabs</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit_2"> <img loading="lazy" src="/resource/images/Immune-Phage-Display-Library-Construction-and-Screening-Flyer-Creative-Biolabs.jpg" width="600" height="500" alt="" /> </div> <a href="/resource/pdf/com/flyers/Immune-Phage-Display-Library-Construction-and-Screening-Flyer-Creative-Biolabs.pdf">Antibody Development by Immune Phage Library</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit_2"> <img loading="lazy" src="/resource/images/Magic-Therapeutic-Antibody-Discovery-Platform-Flyer-Creative-Biolabs.jpg" width="600" height="500" alt="" /> </div> <a href="/resource/pdf/com/flyers/Magic-Therapeutic-Antibody-Discovery-Platform-Flyer-Creative-Biolabs.pdf">Magic™ Therapeutic Antibody Discovery Platform</a> </div> </div> </div>  <div class="tab_cont"> <div class="row pagerow_pt"> <div class="col-md-4"> <div class="pagerow_pt_unit"> <img loading="lazy" src="../static/img/Phage-Display-Technology-as-a-Powerful-Platform-for-Antibody-Drug-Discovery-2.jpg" width="600" height="500" alt="" /> </div> <a href="phage-display-technology-as-a-powerful-platform-for-antibody-drug-discovery.html">Phage Display Technology as a Powerful Platform for Antibody Drug Discovery</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit"> <img loading="lazy" src="/static/img/Therapeutic-Monoclona-Antibody-Therapies-in-Chronic-Autoimmune-Demyelinating-Neuropathies-1.jpg" width="600" height="500" alt="" /> </div> <a href="therapeutic-monoclonal-antibody-therapies-in-chronic-autoimmune-demyelinating-neuropathies.html">Therapeutic Monoclonal Antibody Therapies in Chronic Autoimmune Demyelinating Neuropathies</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit"> <img loading="lazy" src="static/img/4-8-1-overview-of-phage-display-technology-1.jpg" width="600" height="500" alt="" /> </div> <a href="overview-of-phage-display-technology.html">Overview of Phage Display Technology</a> </div> </div> </div> <script> window.addEventListener('load',function(){ // $(function () { $(".tab_nav span").click(function(){ var eqindex=$(this).index(); $(this).addClass("tab_choosebg"); $(this).siblings("span").removeClass("tab_choosebg"); $(".tab_cont").hide().eq(eqindex).show(); }) // }) }); </script> <style> .therapy_ad{position: relative;} .therapy_ad img{ max-width:100%; height:auto; display: block; margin:auto auto 20px auto; } </style><br> <p class="pronote"><i class="fa fa-exclamation-triangle"></i>All listed services and products are For Research Use Only. 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