AAV Services | GENEWIZ to Azenta

<!DOCTYPE html>    <html lang="en">  <head> <title>AAV Services | GENEWIZ to Azenta</title>  <meta charset="utf-8"> <meta http-equiv="X-UA-Compatible" content="IE=edge,chrome=1"> <meta name="viewport" content="width=device-width, initial-scale=1.0"> <meta name="description" content="Utilize Azenta Life Sciences' portfolio of end-to-end adeno-associated virus (AAV) services to streamline your AAV gene therapy research."> <meta name="keywords" content="adeno-associated virus, in vivo gene therapy, cell and gene therapy, AAV gene therapy, AAV vectors, AAV synthesis, AAV plasmids, AAV plasmid preparation, Viral plasmid preparation, Inverted Terminal repeats, ITR sequencing, ITR confirmation"> <meta name="author" content="">  <link rel="shortcut icon" href="favicon.png">   <link rel="stylesheet" href="/assets/plugins/bootstrap/css/bootstrap.min.css"> <link rel="stylesheet" href="/assets/css/style.css?v=1"> <link rel="stylesheet" href="/assets/css/custom.css?v=1"> <link rel="canonical" href="https://www.genewiz.com/Public/Research-Areas/AAV/" /> <link rel="alternate" hreflang="x-default" href="https://www.genewiz.com/Public/Research-Areas/AAV" /> <link rel="alternate" hreflang="en-us" href="https://www.genewiz.com/Public/Research-Areas/AAV" /> <link rel="alternate" hreflang="en-us" href="https://www.genewiz.com/en/Public/Research-Areas/AAV" /> <link rel="alternate" hreflang="en-GB" href="https://www.genewiz.com/en-GB/Public/Research-Areas/AAV" /> <script type="text/javascript" src="/sitecore/shell/controls/lib/prototype/prototype.js"></script> <script type="text/javascript" src="/assets/plugins/jquery/jquery.min.js"></script> <script type="text/javascript"> var jQuery = $; //if (window.location.search.indexOf("sc_mode=edit") > 0) { jQuery.noConflict(); //} </script> <script type="text/javascript" src="/assets/plugins/bootstrap/js/bootstrap.min.js"></script> <script type="text/javascript" src="/assets/plugins/back-to-top.js"></script> <script type="text/javascript" src="/assets/js/app.js"></script>   <script> (function (w, d, s, l, i) { w[l] = w[l] || []; w[l].push( { 'gtm.start': new Date().getTime(), event: 'gtm.js' } ); var f = d.getElementsByTagName(s)[0], j = d.createElement(s), dl = l != 'dataLayer' ? 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href='/en/Public/Services/Next-Generation-Sequencing/RNA-Seq' style='color:#585F69;font-size:14px'>RNA-Seq</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Proteomics' style='color:#585F69;font-size:14px'>Proteomics</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/High-Throughput-Gene-Expression-Screening' style='color:#585F69;font-size:14px'>High-Throughput Gene Expression Screening <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Single-Cell-Sequencing' style='color:#585F69;font-size:14px'>Single-Cell Sequencing</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Exome-Sequencing' style='color:#585F69;font-size:14px'>Exome Sequencing</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Amplicon-Sequencing-Services' style='color:#585F69;font-size:14px'>Amplicon Sequencing Services</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Standalone-NGS-Solutions' style='color:#585F69;font-size:14px'>Sequencing Only<br></a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Whole-Genome-Sequencing' style='color:#585F69;font-size:14px'>Whole Genome Sequencing</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/High-Throughput-Genotyping' style='color:#585F69;font-size:14px'>CRISPR Validation</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Targeted-Resequencing-Panels' style='color:#585F69;font-size:14px'>Targeted Sequencing</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Metagenomics-Solutions' style='color:#585F69;font-size:14px'>Metagenomics </a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Epigenomics' style='color:#585F69;font-size:14px'>Epigenomics</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Antibody-Discovery' style='color:#585F69;font-size:14px'>Antibody Discovery <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Immunogenomics' style='color:#585F69;font-size:14px'>Immunogenomics</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Digital-Spatial-Profiling' style='color:#585F69;font-size:14px'>Digital Spatial Profiling</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/AAV-Genome-Sequencing' style='color:#585F69;font-size:14px'>AAV Genome Sequencing</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Whole-Plasmid-Sequencing-Plasmid-EZ' style='color:#585F69;font-size:14px'>Whole Plasmid Sequencing: Plasmid-EZ <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li></ul></div><div class='col-md-3 '><ul class='list-unstyled'><li><a class='GColor' href='/en/Public/Services/Sanger-Sequencing' style='color:#826ec3;font-size:17px'>Sanger Sequencing</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Sanger-EZ' style='color:#585F69;font-size:14px'>Sanger-EZ <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li><li><a href='/en/Public/Services/Sanger-Sequencing/AAV-ITR' style='color:#585F69;font-size:14px'>AAV-ITR Sanger Sequencing</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Purified-Templates' style='color:#585F69;font-size:14px'>Purified Templates</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Difficult-Template-Sequencing' style='color:#585F69;font-size:14px'>Difficult Template Sequencing</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Direct-Colony-Sequencing' style='color:#585F69;font-size:14px'>Direct Colony Sequencing</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/PCR-Purification' style='color:#585F69;font-size:14px'>PCR Purification</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Primer-Walking' style='color:#585F69;font-size:14px'>Primer Walking</a></li><br><li><a class='GColor' href='/en/Public/Services/Molecular-Genetics' style='color:#826ec3;font-size:17px'>PCR + Sanger Services</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Bacterial-and-Fungal-Identification' style='color:#585F69;font-size:14px'>Bacterial and Fungal Identification</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Mutation-Analysis' style='color:#585F69;font-size:14px'>Mutation Analysis</a></li><li><a href='/en/Public/Services/Molecular-Genetics/SNP-Genotyping' style='color:#585F69;font-size:14px'>SNP Genotyping</a></li><li><a href='/en/Public/Services/Molecular-Genetics/PCR-Plus-Sequencing-CRISPR-Analysis-Package' style='color:#585F69;font-size:14px'>PCR Plus Sequencing CRISPR Analysis Package</a></li><li><a href='/en/Public/Services/Molecular-Genetics/cDNA-Verification' style='color:#585F69;font-size:14px'>Confirmatory Sequencing & Cell Bank Characterization</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Yeast-Colony-Sequencing' style='color:#585F69;font-size:14px'>Yeast Colony Sequencing</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Fragment-Analysis' style='color:#585F69;font-size:14px'>Fragment Analysis</a></li><li><a href='/en/Public/Services/Molecular-Genetics/PCR-Solutions' style='color:#585F69;font-size:14px'>Quantitative PCR & Digital PCR <sup style="color:#826ec3;"><strong>NEW </strong></sup></a></li><li><a href='/en/Public/Services/Molecular-Genetics/Nucleic-Acid-Extraction' style='color:#585F69;font-size:14px'>Nucleic Acid Extraction</a></li></ul></div><div class='col-md-3 '><ul class='list-unstyled'><li><a class='GColor' href='/en/Public/Services/Gene-Synthesis' style='color:#826ec3;font-size:17px'>DNA & RNA Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Gene-Synthesis' style='color:#585F69;font-size:14px'>Gene Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Antibody-DNA-Synthesis' style='color:#585F69;font-size:14px'>Antibody Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/AAV-Plasmid-Synthesis' style='color:#585F69;font-size:14px'>AAV Plasmid Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Gene-Fragments' style='color:#585F69;font-size:14px'>Gene Fragments</a></li><li><a href='/en/Public/Services/Gene-Synthesis/CRISPR-Construct-Synthesis' style='color:#585F69;font-size:14px'>CRISPR Construct Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Synthetic-DNA-Libraries' style='color:#585F69;font-size:14px'>Synthetic DNA Libraries</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Single-Stranded-DNA-Synthesis' style='color:#585F69;font-size:14px'>ssDNA Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/DNA-Cloning' style='color:#585F69;font-size:14px'>DNA Cloning</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Site-Directed-Mutagenesis' style='color:#585F69;font-size:14px'>Site-Directed Mutagenesis<br></a></li><li><a href='/en/Public/Services/Gene-Synthesis/Viral-Package' style='color:#585F69;font-size:14px'>Viral Packaging <sup style="color:#826ec3;"><strong>NEW </strong></sup></a></li><li><a href='/en/Public/Services/Gene-Synthesis/Recombinant-Antibody-Production' style='color:#585F69;font-size:14px'>Recombinant Antibody Production <sup style="color:#826ec3;"><strong>NEW </strong></sup></a></li><li><a href='/en/Public/Services/Gene-Synthesis/Custom-mRNA-Synthesis' style='color:#585F69;font-size:14px'>Custom mRNA Synthesis <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li><br><li><a class='GColor' href='/en/Public/Services/Oligo' style='color:#826ec3;font-size:17px'>Oligo Synthesis Services</a></li><li><a href='/en/Public/Services/Oligo/Overnight-DNA-Oligos' style='color:#585F69;font-size:14px'>Overnight DNA Oligos</a></li><li><a href='/en/Public/Services/Oligo/Custom-DNA-and-RNA-Oligos' style='color:#585F69;font-size:14px'>Custom DNA and RNA Oligos</a></li><li><a href='/en/Public/Services/Oligo/Oligo-Retained-for-Sanger-Sequencing' style='color:#585F69;font-size:14px'>Oligo Retained for Sanger Sequencing</a></li></ul></div><div class='col-md-3 '><ul class='list-unstyled'><li><a class='GColor' href='/en/Public/Services/Plasmid-DNA-Prep' style='color:#826ec3;font-size:17px'>Plasmid DNA Preparation</a></li><li><a href='/en/Public/Services/Plasmid-DNA-Prep/Standard' style='color:#585F69;font-size:14px'>Standard Plasmid DNA Prep</a></li><li><a href='/en/Public/Services/Plasmid-DNA-Prep/Targeted-Yield' style='color:#585F69;font-size:14px'>Targeted Yield Plasmid DNA Prep</a></li><li><a href='/en/Public/Services/Plasmid-DNA-Prep/AAV-Plasmid-Preparation' style='color:#585F69;font-size:14px'>AAV Plasmid Preparation</a></li><br><li><a class='GColor' href='/en/Public/Services/Clinical-Services' style='color:#826ec3;font-size:17px'>PreClinical and Clinical Services</a></li><li><a href='/en/Public/Services/Clinical-Services/Clinical-Services-Overview' style='color:#585F69;font-size:14px'>Clinical Services</a></li><li><a href='/en/Public/Services/Clinical-Services/Microarray-Solutions' style='color:#585F69;font-size:14px'>Microarray Solutions <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Variant-Confirmation' style='color:#585F69;font-size:14px'>CLIA Variant Confirmation (PCR + Sanger)</a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Sanger-Sequencing' style='color:#585F69;font-size:14px'>CLIA Sanger Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Whole-Exome-Sequencing' style='color:#585F69;font-size:14px'>CLIA Whole Exome Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Whole-Genome-Sequencing' style='color:#585F69;font-size:14px'>CLIA Whole Genome Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/Regulatory' style='color:#585F69;font-size:14px'>GLP-Compliant Overview</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-Confirmatory-Sequencing' style='color:#585F69;font-size:14px'>GLP Confirmatory Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-SNP-Mutation-Analysis' style='color:#585F69;font-size:14px'>GLP SNP Mutation Analysis</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-Plasmid-Prep' style='color:#585F69;font-size:14px'>GLP Plasmid Prep</a></li><li><a href='/en/Public/Services/Clinical-Services/Nucleic-Acid-Extraction' style='color:#585F69;font-size:14px'>GLP Nucleic Acid Extraction</a></li><li><a href='/en/Public/Services/Clinical-Services/Biofluid-Processing-Overview' style='color:#585F69;font-size:14px'>Biofluid Processing</a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Integration-Site-Analysis' style='color:#585F69;font-size:14px'>CLIA Integration Site Analysis <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li></ul></div></div></div></li></ul></li><li class='dropdown'><a href='/en/Public/Research-Areas' data-toggle='dropdown' class='dropdown-toggle' style='color:white;height:52px;top:47px;padding-top:0;background-color:transparent;font-size:17px;font-weight:bold;'>RESEARCH AREAS</a><ul class='dropdown-menu'><li class='dropdown'><a class='GColor' href='/en/Public/Research-Areas/AAV' style='font-size:17px'>AAV Services</a><ul class='dropdown-menu'></ul></li><li class='dropdown'><a class='GColor' href='/en/Public/Research-Areas/Agriculture-Food-Science' style='font-size:17px'>Agriculture and Food Science</a><ul class='dropdown-menu'></ul></li><li class='dropdown'><a class='GColor' href='https://web.azenta.com/cell-and-gene-therapy' style='font-size:17px'>Cell and Gene Therapy Solutions <br></a><ul class='dropdown-menu'></ul></li><li class='dropdown'><a class='GColor' href='/en/Public/Research-Areas/Biomarkers' style='font-size:17px'>Biomarkers</a><ul class='dropdown-menu'></ul></li><li class='dropdown'><a class='GColor' href='/en/Public/Research-Areas/Cancer-Research' style='font-size:17px'>Cancer Research</a><ul class='dropdown-menu'></ul></li><li class='dropdown'><a class='GColor' href='/en/Public/Research-Areas/Infectious-Disease' style='font-size:17px'>Infectious Disease</a><ul class='dropdown-menu'></ul></li><li class='dropdown'><a class='GColor' href='/en/Public/Research-Areas/Synthetic-Biology-Biotechnology' style='font-size:17px'>Synthetic Biology</a><ul class='dropdown-menu'></ul></li><li class='dropdown'><a class='GColor' href='/en/Public/Research-Areas/Metagenomics' style='font-size:17px'>Metagenomics</a><ul class='dropdown-menu'></ul></li><li class='dropdown'><a class='GColor' href='/en/Public/Research-Areas/Genome-Editing-and-Engineering' style='font-size:17px'><span>Genome Editing and Engineering</span></a><ul class='dropdown-menu'></ul></li></ul></li><li class='dropdown'><a href='/en/Public/Resources' data-toggle='dropdown' class='dropdown-toggle' style='color:white;height:52px;top:47px;padding-top:0;background-color:transparent;font-size:17px;font-weight:bold;'>RESOURCES</a><ul class='dropdown-menu'><li class='dropdown'><a class='GColor' href='/en/Public/Resources/Payment-Center' style='font-size:17px'>Payment Center</a><ul class='dropdown-menu'></ul></li><li class='dropdown-submenu'><a class='GColor' href='/en/Public/Resources/Sample-Submission-Guidelines' style='font-size:17px'>Sample Submission Guidelines<br></a><ul class='dropdown-menu'><li><a class='GColor' href='/en/Public/Resources/Sample-Submission-Guidelines/Sanger-Sequencing-Sample-Submission-Guidelines' style='font-size:17px'>Sanger Sequencing<br></a></li><li><a class='GColor' 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} .aav-content-box button { border: none; border-radius: 4px; background: #826ec3; padding: 10px 20px; color: #fff; font-size: 15px; letter-spacing: 1px; } .aav-content-box button:hover { background: #6959a1; } @media only screen and (max-width: 768px) { .headline-center { padding-top: 0; } .aav-new-container { text-align: center; margin-top: 0; } .aav-new-content { width: 90%; margin: 0 auto; } .aav-new-content { display: inline-block; } #aav-content-box-right, #ngs-content-box-left { width: 100%; text-align: center; } #aav-content-box-right { margin-top: 20px; } } @media only screen and (max-width: 1170px) { .aav-new-content { width: 90%; margin: 0 auto; } .aav-content-box img { width: 350px; } } </style> <div class="aav-new-container"> <div class="aav-new-content"> <div class="aav-content-box" id="aav-content-box-left"> <h1>Adeno-Associated VIRUS (AAV) Services</h1> <div> </div> <p>As cell and gene therapy research continues to advance across a wide-range of diseases, the need for effective vectors for successful therapy delivery has increased. Identified as an optimal vehicle, adeno-associated virus (AAV) plasmids are the leading viral vector used in <em>in vivo</em> gene therapy clinical trials due to their high efficiency and enhanced safety in humans.</p> <p>As a global leader in genomics services, Azenta has developed unique AAV-ITR sequencing, AAV plasmid synthesis, AAV packaging and AAV plasmid preparation capabilities to support scientists in their <em>in vivo</em> gene therapy research. Azenta’s ground-breaking AAV services also include sequence verification and correction of inverted terminal repeat (ITR) regions, which are crucial for rAAV packaging production. </p> <br /> <a href="mailto:pm@azenta.com"><button>Contact Us</button></a> </div> <div class="aav-content-box" id="aav-content-box-right"> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://www.genewiz.com/en/Public/Services/Gene-Synthesis/AAV-Packaging" target="_blank"><img alt="" src="/-/media/12051-D_AAV-Packaging_WebBanRight2.ashx?la=en&hash=9CB0A1C5CA8EBEA3B0480D4625EF0F50C308FEE6" /></a></div> <a rel="noopener noreferrer" href="https://web.genewiz.com/innovative-solutions-webinar-1" target="_blank"> </a></div> <a rel="noopener noreferrer" href="https://web.genewiz.com/innovative-solutions-webinar-1" target="_blank"> </a></div> </div> <div class="headline-center Rich-Text-Edit-Area"> <link href="https://fonts.googleapis.com/css?family=Roboto:300,400,500,700" rel="stylesheet" /> <style type="text/css"> .covid-banner1 { width: 1170px; height: auto; margin: 0 auto; background-image: url("/-/media/77007-D-0724-Workshops_Medium_Background.ashx"); background-size: cover; background-position: center; text-align: left; padding: 0px 0 0px 0; } .covid-content1 { text-align: center; } .covid-box1 h3 { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; line-height: 115%; font-size: 40px; margin: 0; padding-bottom: 10px; padding-top: 90px; } .covid-box1 h4 { color: #ffffff; text-transform: none; letter-spacing: 1px; line-height: 30px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 35px; margin: 0; margin-bottom: 5px; } .covid-box1 p { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 25px; margin: 0; margin-bottom: 5px; } .covid-box1 button { padding: 8px 19px; color: #826ec3; letter-spacing: 1px; background: #ffffff; border: 2px solid #ffffff; border-radius: 0px; transition: all .2s ease-in-out; font-size: 15px } .covid-box1 button:hover { cursor: pointer; transform: scale(1.1); } .covid-box1 button:focus { outline: 0; } @media only screen and (max-width: 1170px) { .covid-banner { width: 100%; height: auto; background-image: url("/-/media/77007-D-0724-Workshops_Mobile_Background3.ashx"); background-size: cover; background-position: center; text-align: center; padding: 2px 2 2px 2; } .covid-content { width: 90%; margin: 0px 0 0px 0; } .covid-box h3 { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 350; line-height: 115%; font-size: 35px; margin: 0; padding-bottom: 10px; padding-top: 90px; } .covid-box p { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 250; font-size: 15px; margin: 0; margin-bottom: 5px; } } </style> <br /> <div class="covid-banner1"> <div class="covid-content1"> <div class="covid-box1"> <h3>Enhancing Gene Transfer Efficiency:<br /> Viral vs. Non-Viral Methods</h3> <p>Friday, September 20, 2024 | 11:00AM EDT</p> <a href="https://hubs.ly/Q02JnG2s0" target="_blank"> <button><strong> REGISTER NOW</strong></button> </a> <p> </p> </div> </div> </div> <p> </p> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .aav-services { width: 1170px; margin: 0 auto; text-align: center; margin-bottom: 50px; } .service-container { width: 100%; text-align: center; display: flex; justify-content: space-around; flex-wrap: wrap; } .aav-service { width: 49%; text-align: center; padding: 20px 0 75px 0; margin: 4px; position: relative; } .aav-service h3 { margin: 0; margin-bottom: 15px; } .aav-service p { width: 90%; margin: 0 auto; /*margin-bottom: 20px;*/ line-height: 20px; padding: 0; } .aav-service img { padding: 10px; } #aav-service-1 { background: #f0f0f0; } #aav-service-1 span { display: none; } #aav-service-2 { background: #f0f0f0; } #aav-service-3 { background: #f0f0f0; } #aav-service-4 { background: #f0f0f0; } #aav-service-5 { background: #f0f0f0; } .aav-service-btn { background: #826ec3; color: #ffffff; border: 2px solid #826ec3; padding: 10px 20px; -webkit-transition: background-color 0.2s ease-in-out; -moz-transition: background-color 0.2s ease-in-out; -o-transition: background-color 0.2s ease-in-out; transition: background-color 0.2s ease-in-out; position: absolute; left: 39%; bottom: 25px; } .aav-service-btn:hover { background: #6959a1; border: 2px solid #6959a1; color: #fff; padding: 10px 20px; } @media only screen and (max-width: 1170px) { .aav-services { width: 90%; } .aav-service-btn { font-size: 12px; padding: 10px 18px; } } @media only screen and (max-width: 768px) { .service-container { display: block; } .aav-service { width: 100%; margin: 10px 0; padding: 10px 0 20px 0; } .aav-service-btn { position: static; left: 0; right: 0; margin: 0; padding: 10px 20px; } .aav-service p { margin-bottom: 20px; } } </style> <div class="aav-services" id="aav-services"> <h2 style="text-transform: uppercase;">AAV Services</h2> <div class="service-container"> <div class="aav-service" id="aav-service-1"> <div class="aav-service-content"> <img alt="" src="/-/media/Images/Home/icons/Purple-Icons/AAV_ITR_Sequencing_Service_Icon_Purple.ashx?w=100px&la=en&hash=F4C3394C43CC4AAC57228D523516EB23569F0776" width="100px" /> <h3>AAV-ITR Sequencing</h3> <p>Utilizes Sanger protocols to sequence-confirm difficult ITR regions, expediting screening and validation of lead candidates. Whole AAV plasmid sequencing through primer walking is also available.</p> </div> <br /> <a href="/en/Public/Services/Sanger-Sequencing/AAV-ITR" target="_blank"><button class="aav-service-btn">Learn More</button></a> </div> <div class="aav-service" id="aav-service-2"> <div class="aav-service-content"> <img alt="" src="/-/media/Images/Home/icons/Purple-Icons/AAV_Plasmid_Synthesis_Service_Icon_Purple.ashx?w=100px&la=en&hash=AD7217F092A260EB54A173E3656CEDC52215B163" width="100px" /> <h3>AAV Plasmid Preparation</h3> <p>Ensures delivery of intact ITR regions while providing superior quality for mini-to-giga scale AAV vectors.</p> <br /> <a rel="noopener noreferrer" href="/en/Public/Services/Plasmid-DNA-Prep/AAV-Plasmid-Preparation" target="_blank"><button class="aav-service-btn">Learn More</button></a> </div> </div> <div class="aav-service" id="aav-service-3"> <div class="aav-service-content"> <img alt="" src="/-/media/Images/Home/icons/Purple-Icons/AAV_Plasmid_Synthesis_Service_Icon_Purple.ashx?w=100px&la=en&hash=AD7217F092A260EB54A173E3656CEDC52215B163" width="100px" /> <h3>AAV Plasmid Synthesis</h3> <p>Enables synthesis and cloning of transgene expression cassettes into custom AAV vectors. Includes ITR correction to synthesize and clone correct ITR sequences. </p> <br /> <a rel="noopener noreferrer" href="/en/Public/Services/Gene-Synthesis/AAV-Plasmid-Synthesis" target="_blank"><button class="aav-service-btn">Learn More</button></a> </div> </div> <div class="aav-service" id="aav-service-4"> <div class="aav-service-content"> <img alt="" src="/-/media/AAV_Genome_Seq_web_service_Icon110x100_Purple.ashx?w=100px&la=en&hash=017169D6EE24B8C9B00AC5124964037772573300" width="100px" /> <h3>AAV Genome Sequencing</h3> <p>Reimagine quality control standards for rAAV vectors by employing long-read (>10kb) NGS to examine genome integrity, detect heterogeneity, and reveal packaging attributes. Analyze host response utilizing RNA-seq and a variety of other proprietary deep sequencing approaches. </p> <br /> <a rel="noopener noreferrer" href="/en/Public/Services/Next-Generation-Sequencing/AAV-Genome-Sequencing" target="_blank"><button class="aav-service-btn">Learn More</button></a> </div> </div> <div class="aav-service" id="aav-service-5"> <div class="aav-service-content"> <img alt="" src="/-/media/Images/Home/icons/Purple-Icons/AAV-Packaging-Purple.ashx?w=100px&la=en&hash=8C595ECDB446C2263DBA8EBCF3A29261BC60DFB2" width="100px" /> <h3>AAV Packaging</h3> <p>Unlock the full potential of cell or gene therapy by overcoming the instability of AAV vectors with GENEWIZ's AAV packaging workflow. Our proprietary AAV plasmid synthesis process offers industry-leading project quality, completion time, and flexibility, ensuring researchers can harness the powerful benefits of this technology effectively.</p> <br /> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://www.genewiz.com/en/Public/Services/Gene-Synthesis/AAV-Packaging" target="_blank"><button class="aav-service-btn">Learn More</button></a> </div> </div> </div> </div> </div> <script type="text/javascript" src="/assets/plugins/counter/jquery.counterup.min.js"></script> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".row .category").find("img").addClass("img-responsive"); }); </script> <div class='Component-Frame pull-center Multi-column-TextBullet-Layout Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-0 padding-bottom-10'> <div class="container"> <div class="headline-center"> <h2>AAV ADVANTAGES</h2> </div> <div class="headline-left"> </div> <div class="row category"> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> Low cell toxicity as it does not integrate into host genome </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> Low immunogenicity and non-pathogenic with minimal immune host response </div> </div> </div> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> High levels of <em>in vivo</em> gene expression </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> High transduction efficiency across a broad range of target tissues </div> </div> </div> </div> </div> </div><div class="headline-center Rich-Text-Edit-Area"> <link href="https://fonts.googleapis.com/css?family=Roboto:300,400,500,700" rel="stylesheet" /> <style type="text/css"> .covid-banner { width: 1170px; height: auto; margin: 0 auto; background-image: url("/-/media/36004-D_ISBER-Webinar_WebBanFull-Lrg_Background.ashx"); background-size: auto; background-position: center center; text-align: left; padding: 0px 0 0px 0; } .covid-content { text-align: center; } .covid-box h3 { color: #ffffff; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 500; line-height: 100%; font-size: 50px; margin: 0; padding-bottom: 15px; } .covid-box h4 { color: #ffffff; text-transform: none; letter-spacing: 1px; line-height: 40px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 30px; margin: 0; margin-bottom: 5px; } .covid-box p { color: #000000; text-transform: none; letter-spacing: 1px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 15px; margin: 0; margin-bottom: 5px; } .covid-box button { padding: 8px 19px; color: #826ec3; letter-spacing: 1px; background: #ffffff; border: 2px solid #ffffff; border-radius: 3px; transition: all .2s ease-in-out; font-size: 15px } .covid-box button:hover { cursor: pointer; transform: scale(1.1); } .covid-box button:focus { outline: 0; } @media only screen and (max-width: 1170px) { .covid-banner { width: 100%; height: auto; } .covid-content { width: 90%; margin: 0 auto; } } </style> <br /> <div class="covid-banner"> <div class="covid-content"> <div class="covid-box"> <br /> <h3>Webinar: Cold Storage and Sample Integrity</h3> <h4>In this webinar with ISBER, biorepository and cryopreservation experts discuss the fundamental elements to achieve consistency and reproducibility in research.</h4> <br /> <a href="https://hubs.ly/Q01BGV_r0" target="_blank"> <br /> <button> REGISTER NOW </button> <br /> </a> <p> </p> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .plasmid-container { background: #f0f0f0; } .plasmid-system { width: 1170px; margin: 0 auto; margin-bottom: 50px; padding: 20px; } .plasmid-system p { padding: 0 40px 0 40px; text-align: left; } .plasmid-system img { text-align: center; max-width: 800px; width: 100%; height: auto; } .plasmid-system h2 { text-align: center; } @media only screen and (max-width: 1700px) { .plasmid-system { width: auto; } </style> <div class="plasmid-container"> <div class="plasmid-system"> <h2>AAV SYSTEM</h2> <p>Adeno-associated virus (AAV) is one of the most actively investigated gene therapy vehicles. AAV has a linear single-stranded DNA (ssDNA) genome of approximately 4.7 kilobases (kb), with two 145 nucleotide-long inverted terminal repeats (ITR) at the termini which are crucial for rAAV production. </p> <img src="/-/media/Images/Services/AAV/AAV_Overview_Webpage_Workflow_Graphic.ashx?la=en&hash=071DFE2D4250EF604159B5A366FFFDD3649611D8"> <p><strong>Figure 1.</strong> An AAV transfer plasmid containing the modified/transgene expression cassette with intact ITRs is generated and cloned into a plasmid along with Rep/Cap and helper plasmids, which are co-transfected into cell lines for virus packaging and rAAV production.</p> </div> </div> </div> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".SingleIcon").find("img").addClass("img-responsive center-block"); }); </script> <div class="process-v1 icon-nav Component-BackGround-Default margin-top-0 margin-bottom-0 padding-top-10 padding-bottom-0"> <div class="container"> <div class="headline-center"> <h2>Features & Benefits</h2> <p></p> </div> <ul class="list-unstyled row "> <li class='col-sm-4'> <div class='SingleIcon headline-center Component-BackGround-Defaultmargin-top-0 margin-bottom-0 padding-top-10 padding-bottom-0'> <div class="row"> <img src="/-/media/Fast-Turnaround-Purple_cut.ashx?h=110&la=en&w=210&hash=09F1B024BE8A42CF5AED9BD5A600C9533795D640" alt="Building" width="210" height="110" title="Fast Turnaround icon small" /> </div> <div class="row"> <br /> <strong>Rapid Turnaround Times</strong> </div> <div class="row"> Starting at just 5 business days </div> </div> </li>  <li class='col-sm-4'> <div class='SingleIcon headline-center Component-BackGround-Defaultmargin-top-0 margin-bottom-0 padding-top-10 padding-bottom-0'> <div class="row"> <img src="/-/media/Real-Time-Updates-Purple_cut.ashx?h=110&la=en&w=210&hash=69C53E8C7921055F266D5EBD62D78EB6BD71B831" alt="Building" width="210" height="110" title="Real Time Updates icon small" /> </div> <div class="row"> <br /> <strong>Real-Time Project Updates</strong> </div> <div class="row"> Through our online system </div> </div> </li>  <li class='col-sm-4'> <div class='SingleIcon headline-center Component-BackGround-Defaultmargin-top-0 margin-bottom-0 padding-top-10 padding-bottom-0'> <div class="row"> <img src="/-/media/Consulative-Services-Purple_cut.ashx?h=110&la=en&w=210&hash=B83D43C49F21B6B5A9132834999A286164EF3F7D" alt="Building" width="210" height="110" title="Consultative Services Icon small" /> </div> <div class="row"> <br /> <strong>Ph.D.-Level Project Managers</strong> </div> <div class="row"> Available at each step </div> </div> </li>  </ul> <div class="row headline-center"> </div> </div> </div> <script type="text/javascript" src="/assets/plugins/counter/jquery.counterup.min.js"></script> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".row .category").find("img").addClass("img-responsive"); }); </script> <div class='Component-Frame pull-center Multi-column-TextBullet-Layout Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-0 padding-bottom-10'> <div class="container"> <div class="headline-center"> </div> <div class="headline-left"> <br /> </div> <div class="row category"> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em style="font-size: 50px;" class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></em><br></div> <div class="content-boxes-in-v3"> End-to-end DNA AAV solutions with ITR sequencing verification, transgene synthesis, and plasmid preparation </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> Low immunogenicity and non-pathogenic with minimal immune host response </div> </div> </div> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em style="font-size: 50px;" class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></em><br /></div> <div class="content-boxes-in-v3"> Robust quality control ensures AAV plasmids are delivered with intact ITRs confirmed via AAV-ITR sequencing </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em style="font-size: 50px;" class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></em><br /></div> <div class="content-boxes-in-v3"> Convenient online ordering directly through your CLIMS account for all services </div> </div> </div> </div> </div> </div><div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> body { margin: 0; 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background-color: #fff; padding: 15px; margin: 0;"> <div class="tech-resources-container"> <h2>Technical Resources</h2> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/11001_AAV-ITR-Seq_ATN-Thumbnail-1.ashx?h=160&w=220&la=en&hash=287F8D807B38899AE7052BC1A95DFA4E225CCD7E" style="height: 160px; width: 220px;" /> </div> <div class="resource resource-right"> <h3>Tech Note: Reading Through the Inverted Terminal Repeats (ITRs) of Adeno-associated Virus (AAV)</h3> <p>Azenta has developed a high-quality, direct Sanger sequencing method that reads through both intact and commonly mutated inverted terminal repeat (ITR) regions of adeno-associated virus (AAV). This method is an effective tool for assessing the integrity of ITRs in AAV plasmids. <br /> <a href="https://web.genewiz.com/aav-itr-tech-note" target="_blank"> <button>Download</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/12051-D_AAV-Packaging_TechResource-Thumb.ashx?h=160&w=220&la=en&hash=B13220BDC7AA1F511A6C578121266C42359E7B9E" style="height: 160px; width: 220px;" /> </div> <div class="resource resource-right"> <h3>Tech Note: An Efficient and Reliable Approach to AAV Packaging</h3> <p>While AAV is a powerful vehicle for gene delivery, unstable ITRs threaten the fidelity of rAAV genomes. Loss of ITR integrity diminishes the packaging efficiency of rAAV particles and thus viral titer. In this tech note, discover the effect of partial and full ITR deletions on AAV packaging and how to reliably identify and effectively correct them. <br /> <a href="https://www.azenta.com/resources/efficient-and-reliable-approach-aav-packaging" target="_blank"> <button>Download</button> </a> </p> </div> </div> </div> </div> </div> <link rel="stylesheet" href="/assets/css/footers/footer-v1.css?v=1"> <div class="footer-v1"> <div class="footer"> <div class="container text-center"> <div class="row"> <div class="col-md-12"> <span class="icon-Solid-Science-tagline"> <p style="font-size:35px; text-align: center;color: white;">GENEWIZ from Azenta Life Sciences</p> <p style="text-align: center;"><a href="https://www.azenta.com/" target="_blank"><img src="https://web.azenta.com/hubfs/ARCHIVE/Azenta%20Logo%20and%20Brand%20Assets/Azenta%20Logos/Azenta_Logo_Primary_FullColor_WhiteType_RGB.png" width="250"></a></p> </span> </div> </div> </div>  <div class="container text-center socialicons"> <div 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