Cultrex UltiMatrix Reduced Growth Factor Basement Membrane Extract (BME001-05) by R&D Systems, Part of Bio-Techne

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class="dropdown-item text-wrap" data-drupal-link-system-path="node/531">Single Cell Western</a> <a href="/instruments" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6731">View All Instruments</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Applications<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/methods/bioprocessing" class="dropdown-item text-wrap">Bioprocessing</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Research Areas<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/research-areas/cell-and-gene-therapy" class="dropdown-item text-wrap" data-drupal-link-system-path="node/51">Cell & Gene Therapy</a> <a href="/applications/immuno-oncology-clinical" class="dropdown-item text-wrap" 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Controls</a> <a href="/diagnostics/controls-reagents/rnascope-ish-probe-high-risk-hpv" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5981">RNAscope™ ISH Probe High Risk HPV</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Kits<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/amplidex-genetic-testing-kits" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5581">Genetics</a> <a href="/diagnostics/quantidex-oncology-testing-kits" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5586">Oncology</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Technology<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/exosome" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5486">Exosome Platform</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> OEM Services<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/ivd-assay-development" class="dropdown-item text-wrap" data-drupal-link-system-path="node/10881">IVD Assay Development</a> <a href="/diagnostics/ivd-contract-manufacturing" class="dropdown-item text-wrap" data-drupal-link-system-path="node/7381">IVD Contract Manufacturing</a> <a href="/diagnostics/molecular-quality-controls/oem-and-commercial-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6781">Molecular Diagnostic OEM Services</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Companion Diagnostics<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/companion-diagnostics" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5631">Companion Diagnostics Services</a> <a href="/diagnostics/companion-diagnostics/multiomics" class="dropdown-item text-wrap" data-drupal-link-system-path="node/10616">Multiomics Platform</a> </div> </div> </div> </div> </div> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown-services" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false"> Services <span class="icon-bt-icon-caret-cropped menu-caret js-caret d-inline-block"></span> </a> <div class="dropdown-menu dropdown-menu-services" aria-labelledby="navbarDropdown-services"> <div class="row"> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Custom Quotes<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/services/bulk-quotes" class="dropdown-item text-wrap" data-drupal-link-system-path="services/bulk-quotes">Bulk Quote Discounts</a> <a href="/services/custom-formulation" class="dropdown-item text-wrap" data-drupal-link-system-path="services/custom-formulation">Custom Formulation Quotes</a> <a href="/reagents/rnascope-ish-technology/probes/custom" class="dropdown-item text-wrap" data-drupal-link-system-path="node/12571">RNAscope Custom Probe Design Quote</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Custom Services<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/services/custom-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/1891">All Custom Services</a> <a href="/services/custom-antibody-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/906">Custom Antibody Services</a> <a href="/services/custom-cell-and-gene-therapy-services" class="dropdown-item text-wrap" 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href="/services/instrument-services/simple-western-service-plans" class="dropdown-item text-wrap" data-drupal-link-system-path="node/3181">Simple Western Service Plans</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Licensing<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/services/biotechnology-licensing-partnering" class="dropdown-item text-wrap" data-drupal-link-system-path="node/1101">Biotechnology Licensing and Partnering</a> </div> </div> </div> </div> </div> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown-resources" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false"> Resources <span class="icon-bt-icon-caret-cropped menu-caret js-caret d-inline-block"></span> </a> <div class="dropdown-menu dropdown-menu-resources" aria-labelledby="navbarDropdown-resources"> <div class="row"> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Product & Instrument Tools<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/resources/cofa-finder-tool" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/cofa-finder-tool">Certificate of Analysis (COA) Finder Tool</a> <a href="/resources/calculators" class="dropdown-item text-wrap" data-drupal-link-system-path="node/7416">Calculators</a> <a href="/resources/flow-cytometry-panel-builder" class="dropdown-item text-wrap" data-drupal-link-system-path="node/8821">Flow Cytometry Panel Builder</a> <a href="/luminex-assay-customization-tool" class="dropdown-item text-wrap" data-drupal-link-system-path="luminex-assay-customization-tool">Luminex Assay Customization Tool</a> <a href="/resources/product-insert-lookup-tool" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/product-insert-lookup-tool">Product Insert Lookup 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class="mb-2 o___product-summary"><div id="pp-page-title-brand-wrapper"> <div id="header-subheader" data-section="section-base--header-subheader"> <div class="row" id="pp-page-title-brand-wrapper"> <div class="col-lg-9 col-md-12"> <h1 class="font-weight-bold"> Cultrex UltiMatrix Reduced Growth Factor Basement Membrane Extract <span class="rounded align-middle p-1 badge best-seller" title="Best Seller">Best Seller</span> </h1> <h2 class="font-weight-normal h4"> R&D Systems, part of Bio-Techne <span id="product-code"> | Catalog # <span class="font-weight-bold">BME001-05</span> </span> </h2> <div id="pp-reviews-citations-row"> <div id="header_left" class="row"> <div id="reviews_quick_link_wrapper" class="col-auto d-flex align-self-center"> <div id="reviews_quicklink"> <a href="#reviews" id="review_count_quicklink" class="reviews" data-tab="2"> <span class="review_stars review_stars_5"></span> <span class="font-weight-bold"></span> (6) </a> </div> </div> <div id="citation_quick_link_wrapper" class="col-auto d-flex align-self-center"> <div id="citation_quicklink"> <a href="#citations" id="citation_count" data-tab="2" class="citations"> <span class="icon-icon-product-citations d-none d-lg-inline pr-1"></span> Citations (8)  </a> </div> </div> <div class="col-auto d-flex align-self-center"> <div> <a href="#product-documents" class="datasheet_link product-documents" rel="nofollow">Product Datasheet / COA / SDS</a> </div> </div> </div> </div> <div id="new_version_available"> </div> <div class="row mb-2" id="extended-description"> <div class="col-md-10 extended-description-wrapper" id="extended-description-wrapper"> A basement membrane matrix hydrogel with compositional enhancements and performance benefits for 2D and 3D cell culture applications. </div> </div> </div> <div class="col-lg-3 col-md-12"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-rnd-systems.svg" alt="R&D Systems, part of Bio-Techne" class="d-flex h-100 w-100"> </div> </div> </div> </div> <div id="product_alerts_wrapper"><article class="product-data-wrapper"> </article> </div> <div id="promotion_description"><article class="product-data-wrapper"> Try it on your cultures! <a class="orange_button" target="_blank" href="https://www.bio-techne.com/reagents/cell-culture-reagents/cultrex-bme-and-ecm-proteins/sample-request">Request a Sample of Cultrex UltiMatrix RGF BME</a> </article> </div> <div class="datapoint_list" id="key_details"><h3>Key Product Details</h3><table class=" table" id="key_details_table_wrapper"><tr class="product-data"> <td class="product-data-label py-2 align-top text-md-left">Features: </td> <td class="product-data-value py-2 align-top w-75 text-break"> Cultrex UltiMatrix RGF BME is an optimized and effective growth scaffold for organoid, pluripotent stem cell, and advanced 3D cell culture applications. It can be used undiluted or diluted to a specific protein concentration. </td> </tr> <tr class="product-data"> <td class="product-data-label py-2 align-top text-md-left">Key Benefits: </td> <td class="product-data-value py-2 align-top w-75 text-break"> <html><body><ul class="mt-4"><li>Designed for organoid and stem cell culture</li><li>Protein composition and storage modulus optimized for 3D applications</li><li>Qualified for dome formation</li><li>Lot-to-lot performance consistency</li><li>High protein concentration</li><li>Low endotoxin profile</li></ul></body></html> </td> </tr> </table> </div> </div> <div class="row border-top mb-2" id="images_atc_orderinfo_wrapper"><div class="col-xl-9 mt-2" id="image_atc_keydetails_wrapper"><div class="row" id="image_atc_wrapper"><div class="col-xl-4 order-xl-first" id="image_wrapper"><article class="product-data-wrapper"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="rnd"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="https://resources.bio-techne.com/images/products/bme001-05_cultrex-ultimatrix-reduced-growth-factor-basement-membrane-matrix-922024145924.jpg" alt="Human Descending Colon Organoid Cultured Using Cultrex UltiMatrix RGF BME" title="Figure 1. Goblet Cells in Adult Stem Cell-derived Human Descending Colon Organoids." data-caption="Adult stem cells isolated from human descending colon were embedded in Cultrex UltiMatrix RGF BME and cultured in growth medium for 30 days. Organoids were fixed and stained with MUC2 (green; Catalog # NBP2-44431), to visualize intestinal goblet cells, and counterstained for E-Cadherin (red; Catalog # <a class="NoLineLink" href="/p/antibodies/human-mouse-e-cadherin-antibody_af748" target="_self">AF748</a>) and DAPI (blue; Catalog # <a class="NoLineLink" href="/p/fluorescent-probes-dyes/dapi_5748" target="_self">5748</a>)." data-badges="" data-application="N/A" width="250" height="250" fetchpriority=high as=image class="search_result_thumbnail_image" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> <span class="image-count-text align-middle">(9)</span> </div> </div> </div> <i data-src="https://resources.bio-techne.com/images/products/bme001-05_cultrex-ultimatrix-reduced-growth-factor-basement-membrane-matrix-data-92202415929.jpg" data-alt="A graph of endotoxin levels and protein concentrations for 20 recent lots of Cultrex UltiMatrix" title="Figure 2. Cultrex UltiMatrix shows improved lot-to-lot consistency with low endotoxin and high protein concentration across 20 recent lots of Cultrex UltiMatrix." data-caption="The brown lines represents the endotoxin levels measured in 20 recent lots of Cultrex UltiMatrix. The graph shows consistently low levels of endotoxin across the lots. Cultrex UltiMatrix is specifically designed to maintain low endotoxin levels. The blue lines represents the protein concentration measured in the same 20 lots of Cultrex UltiMatrix. The graph demonstrates the consistency of protein concentration from lot to lot, highlighting the product's ability to maintain higher, constant protein levels. Cultrex UltiMatrix is designed to provide consistent protein concentration for reliable experimental outcomes." data-badges="" data-application="N/A" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/bme001-05_cultrex-ultimatrix-reduced-growth-factor-basement-membrane-matrix-922024153039..png" data-alt="" title="Figure 3. Cultrex UltiMatrix RGF Basement Membrane Extract (BME) Displays Consistent Lot-to-Lot ECM Composition, High Tensile Strength, and Gel Rates." data-caption="A) Simple Western analysis for Entactin, Laminin, and Collagen IV across two lots of both Cultrex UltiMatrix RGF BME (Catalog # BME001) and an alternate commercial BME matrix. Cultrex UltiMatrix RGF BME shows consistent expression of Entactin, compared to the alternate ECM matrix. B) The tensile strength (elastic modulus) dynamics across two lots of Cultrex UltiMatrix RGF BME and an alternate commercial BME matrix. The elastic modulus was measured at increasing temperatures to determine tensile strength and gelling rate across two independent lots of Cultrex UltiMatrix RGF BME (Catalog # BME001) and the alternate BME matrix. Cultrex UltiMatrix RGF BME was found to display consistent gelling rates and high tensile strength (storage modulus) compared to the alternate commercial BME matrix." data-badges="" data-application="N/A" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/bme001-05_cultrex-ultimatrix-reduced-growth-factor-basement-membrane-matrix-922024153428..jpg" data-alt="Human Descending Colon Organoids Grown in Cultrex UltiMatrix RGF BME" title="Figure 4. Human Descending Colon Organoids Grown in Cultrex UltiMatrix RGF BME." data-caption="Adult stem cells isolated from human descending colon were embedded in Cultrex UltiMatrix RGF BME and cultured in growth medium for 30 days prior to imaging. <b>A)</b> Brightfield image of descending colon organoid showing tissue invagination and epithelial layer formation. <b>B, C)</b> Descending colon organoids were stained with Chromogranin A (green; Catalog # <a class="NoLineLink" href="/p/antibodies/human-chromogranin-a-antibody-948128_mab90981" target="_self">MAB90981</a>), to visualize intestinal enteroendocrine cells, and counterstained for E-Cadherin (red; Catalog # <a class="NoLineLink" href="/p/antibodies/human-mouse-e-cadherin-antibody_af748" target="_self">AF748</a>) and DAPI (blue; Catalog # <a class="NoLineLink" href="/p/fluorescent-probes-dyes/dapi_5748" target="_self">5748</a>)." data-badges="" data-application="N/A" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/bme001-05_cultrex-ultimatrix-reduced-growth-factor-basement-membrane-matrix-922024153735..jpg" data-alt="Human Lung Organoids Grown in Cultrex UltiMatrix RGF BME." title="Figure 5. Human Lung Organoids Grown in Cultrex UltiMatrix RGF BME." data-caption="Adult stem cells isolated from human lung biopsy tissue were embedded in Cultrex UltiMatrix RGF BME and cultured in lung organoid expansion medium where they were able to differentiate and exhibit key markers for various lung cell types. <b>A)</b> Lung organoids stained with anti-Cytokeratin 5 (KRT5) (green; Catalog # NB110-56916) and with anti-p63/ TP73L (red; Catalog # <a class="NoLineLink" href="/p/antibodies/human-p63-tp73l-antibody_af1916" target="_self">AF1916</a>) to visualize basal cells. <b>B)</b> Lung organoids stained with anti-Podoplanin (PDPN) (green; Catalog # NB600-1015) to visualize alveolar type I cells. <b>C)</b> Quantification of lung organoid size across organoids grown in Cultrex UltiMatrix RGF BME from 4 different lots shows high performance consistency of the matrix in this application." data-badges="" data-application="N/A" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/bme001-05_cultrex-ultimatrix-reduced-growth-factor-basement-membrane-matrix-922024154546..jpg" data-alt="Cultrex UltiMatrix BME iPSC Expansion Compared to Competitor" title="Figure 6. Robust Induced Pluripotent Stem Cell (iPSC) Expansion Compared to an Alternate BME Supplier." data-caption="<b>(A)</b> Human iPSCs were passaged in 6 well plates (seeded at 7.5x10<sup>5</sup> cells/well) using either Cultrex UltiMatrix RGF BME (purple) or an Alternate BME Supplier (grey) as a substrate. At each passage cells were harvested and counted by hemocytometer. Cultrex UltiMatrix BME showed robust iPSC expansion compared to the Alternate BME Supplier. <b>B)</b> iPSC cells grown on Cultrex UltiMatrix BME retained expression of the pluripotency marker SSEA-4, as labeled using a Fluorescein conjugated SSEA-4 antibody (Catalog # FAB1435)." data-badges="" data-application="N/A" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/bme001-05_cultrex-ultimatrix-reduced-growth-factor-basement-membrane-matrix-922024154913..jpg" data-alt="Improved Spheroid (Acinar) Maturation and Consistency Compared to Other Commercial Matrices" title="Figure 7. Improved Spheroid (Acinar) Maturation and Consistency Compared to Other Commercial Matrices." data-caption="Single MCF10A cells were cultured for in Cultrex UltiMatrix BME, Cultrex BME, Type R1 (3433-005-R1), or an alternate BME supplier. Over 12 days in culture, cells differentiated into luminal structures called “acini” and were quantified for acinar area. Cultrex UltiMatrix BME showed increased acinar maturation (larger area) compared to Cultrex BME, Type R1 and more consistent acinar area compared to the alternate BME supplier." data-badges="" data-application="N/A" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/bme001-05_cultrex-ultimatrix-reduced-growth-factor-basement-membrane-matrix-922024155550..jpg" data-alt="Cultrex UltiMatrix RGF BME has Optimized Extracellular Matrix Composition and Soluble Protein" title="Figure 8. Optimized Extracellular Matrix Composition and Soluble Protein." data-caption="<b>A)</b> Multiple lots of Cultrex UltiMatrix RGF BME were run by Simple Western to determine extracellular matrix protein composition. The Western blots show the matrix to have high lot-to-lot consistency of protein composition. <b>B)</b> Cultrex UltiMatrix RGF BME showed a lower amount of aggregated protein in the matrix solution compared to Cultrex BME, Type R1. Lower protein aggregation indicates a higher amount of soluble protein in solution, which improves protein crosslinking and matrix integrity in culture." data-badges="" data-application="N/A" data-width="250" data-height="250" class="none"></i> <i data-src="https://resources.bio-techne.com/images/products/bme001-05_cultrex-ultimatrix-reduced-growth-factor-basement-membrane-matrix-922024155851.png" data-alt="Cultrex UltiMatrix Reduced Growth Factor Basement Membrane Extract (Catalog # BME001)" title="Cultrex UltiMatrix Reduced Growth Factor Basement Membrane Extract (Catalog # BME001)." data-caption="" data-badges="" data-application="N/A" data-width="250" data-height="250" class="none"></i> </div> </div> </article> </div> <div class="col-xl-8" id="atc_wrapper"><article class="product-data-wrapper"> <div class="atc-container"> <div class="atc-block" data-type="ds" data-blockid="ProductCart" > <form action="/commerce/addtocart" method="post" class="add-to-cart-block" 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class="nav-item m-0"> <a class="nav-link py-0 px-2 product-documents datasheet_static_link" href="#product-documents" id="product-documents_li" >Product Datasheets</a> </li> </div> <div id="product-summary" class="nav-link px-0" data-anchor-label="Product Summary"><div id="product_title_summary"><h3>Summary for Cultrex UltiMatrix Reduced Growth Factor Basement Membrane Extract</h3><h4> Why use Cultrex UltiMatrix RGF BME Matrix for Organoid and Pluripotent Stem Cell Culture? </h4> <article class="product-data-wrapper"> <p>Cultrex UltiMatrix Reduced Growth Factor (RGF) Basement Membrane Extract (BME) is a soluble form of basement membrane that provides high storage modulus, enhanced levels of entactin/nidogen, elevated protein concentration, and robust clarity and purity that ensures that the hydrogel contains the maximal amount of soluble extracellular matrix proteins. These compositional and concentration enhancements make Cultrex UltiMatrix RGF BME the choice hydrogel matrix with proven efficacy in organoid cell culture, induced pluripotent stem cell expansion and differentiation, spheroid formation, and other 2D and 3D cell culture applications. <br /><br />Cultrex UltiMatrix RGF BME is purified from mouse Engelbreth-Holm-Swarm (EHS) tumor, Cultrex UltiMatrix RGF BME is viscous at 2-8 °C and gels at 37 °C to form a reconstituted basement membrane.<br /><br /><iframe width="560" height="315" src="https://www.youtube.com/embed/0QbkM1OQ13I" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture" allowfullscreen></iframe></p> </article> <h4> How Do I Use Cultrex UltiMatrix RGF BME? </h4> <article class="product-data-wrapper"> Basement membrane matrix hydrogels play an essential role in developing complex <em>in vitro</em> and <em>ex vivo</em> cell and tissue culture models. By providing an optimized mixture of laminin, collagen IV, entactin/nidogen, and heparan sulfate proteoglycans, hydrogels like Cultrex UltiMatrix RGF BME provide native cell-matrix interactions that influence cell behavior and native developmental programs that promote complex cell culture and 3D tissue generation <em>in vitro</em>.<br /><br />To preserve its performance benefits, Cultrex UltiMatrix RGF BME should be thawed overnight at 2-8 °C; the thawed hydrogel solidifies quickly at temperatures above 15 °C. When thawing, aliquoting, and diluting Cultrex UltiMatrix RGF BME, keep it on ice to prevent untimely gelling.<br /><br /><b>Protocols using Cultrex UltiMatrix RGF BME:</b> <br /><br />Video of <a href="https://www.youtube.com/embed/mhja5Rv9bqk" target="_blank"> Cultrex BME Best Practices and Protocols</a>. <br />Protocol for <a href="https://www.bio-techne.com/resources/protocols-troubleshooting/mouse-enteric-organoid-culture" target="_blank"> Mouse Enteric Organoid Culture</a>.<br />Protocol for <a href="https://www.bio-techne.com/resources/protocols-troubleshooting/human-gastric-organoid-culture" target="_blank"> Human Gastric Organoid Culture</a>.<br />Protocol for <a href="https://www.bio-techne.com/resources/protocols-troubleshooting/human-liver-organoid-culture" target="_blank"> Human Liver Organoid Culture</a>.<br />Protocol for <a href="https://www.bio-techne.com/resources/protocols-troubleshooting/human-lung-organoid-culture" target="_blank"> Human Lung Organoid Culture</a>.<br />Protocol for <a href="https://www.bio-techne.com/resources/protocols-troubleshooting/human-intestinal-organoid-culture" target="_blank"> Human Intestinal Organoid Culture</a>.<br />Protocol for <a href="https://www.rndsystems.com/resources/protocols/harvesting-organoids-biochemical-analysis" target="_blank"> Harvesting Organoids for Biochemical Analysis</a>. </article> </div> </div> <div id="product-specifications" class="nav-link px-0" data-anchor-label="Product Specifications"><table class="tablesaw tablesaw-stack table-bordered table" id="product_specification_wrapper"><h3>Product Specifications for Cultrex UltiMatrix Reduced Growth Factor Basement Membrane Extract</h3><tr class="product-data"> <td class="product-data-label font-weight-bold p-3 align-top text-md-right w-25">Species</td> <td class="product-data-value p-3 px-4 text-break"> Mouse </td> </tr> <tr class="product-data"> <td class="product-data-label font-weight-bold p-3 align-top text-md-right w-25">Source</td> <td class="product-data-value p-3 px-4 text-break"> Murine Engelbreth-Holm-Swarm (EHS) tumor </td> </tr> <tr class="product-data"> <td class="product-data-label font-weight-bold p-3 align-top text-md-right w-25">Protein Concentration</td> <td class="product-data-value p-3 px-4 text-break"> 10-12 mg/mL as determined by Lowry assay </td> </tr> <tr class="product-data"> <td class="product-data-label font-weight-bold p-3 align-top text-md-right w-25">Sterility Testing</td> <td class="product-data-value p-3 px-4 text-break"> No bacterial or fungal growth detected following 14 days in culture </td> </tr> <tr class="product-data"> <td class="product-data-label font-weight-bold p-3 align-top text-md-right w-25">Cell Culture Testing</td> <td class="product-data-value p-3 px-4 text-break"> <b>Organoid Culture</b> - Supports culture of human small intestine or colon organoids. Supports mouse intestinal organoids.<br /><br /><b>Pluripotent Stem Cell Culture</b> - Promotes the attachment of human induced pluripotent stem cells and effectively maintains them in a pluripotent state under a feeder-free conditions. <br /><br><b>Dome Formation</b> - Supports dome formation at a concentration of 8 mg/mL and maintains dome structure for 7 days.<br /><br /><b>Gelling Assay</b> - Gels in less than 30 minutes at 37 °C and maintains the gelled form in culture medium for a minimum of 7 days at 37 °C. <br /><br> </td> </tr> <tr class="product-data"> <td class="product-data-label font-weight-bold p-3 align-top text-md-right w-25">Viral Testing</td> <td class="product-data-value p-3 px-4 text-break"> Tested negative by PCR test for a total of 31 organisms and viruses, including: mycoplasma, 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, and 13 additional murine infectious agents including LDEV. </td> </tr> </table> </div> <div id="scientific-data" class="nav-link px-0" data-anchor-label="Scientific Data"><table class="product-data-wrapper tablesaw tablesaw-stack table" id="data_image_table_wrapper"><h3>Scientific Data Examples for Cultrex UltiMatrix Reduced Growth Factor Basement Membrane Extract</h3><article class="product-data-wrapper"> <tr class="row m___product-data-examples"> <td class="border-0"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand=""> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/bme001-05_cultrex-ultimatrix-reduced-growth-factor-basement-membrane-matrix-922024145924.jpg" data-srcset="https://resources.bio-techne.com/images/products/bme001-05_cultrex-ultimatrix-reduced-growth-factor-basement-membrane-matrix-922024145924.jpg" alt="Human Descending Colon Organoid Cultured Using Cultrex UltiMatrix RGF BME" title="Figure 1. Goblet Cells in Adult Stem Cell-derived Human Descending Colon Organoids." data-caption="Adult stem cells isolated from human descending colon were embedded in Cultrex UltiMatrix RGF BME and cultured in growth medium for 30 days. Organoids were fixed and stained with MUC2 (green; Catalog # NBP2-44431), to visualize intestinal goblet cells, and counterstained for E-Cadherin (red; Catalog # <a class="NoLineLink" href="/p/antibodies/human-mouse-e-cadherin-antibody_af748" target="_self">AF748</a>) and DAPI (blue; Catalog # <a class="NoLineLink" href="/p/fluorescent-probes-dyes/dapi_5748" target="_self">5748</a>)." data-badges="" data-application="N/A" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </td> <td class="w-75 border-0 align-top"> <p> <strong>Figure 1. Goblet Cells in Adult Stem Cell-derived Human Descending Colon Organoids.</strong> Adult stem cells isolated from human descending colon were embedded in Cultrex UltiMatrix RGF BME and cultured in growth medium for 30 days. Organoids were fixed and stained with MUC2 (green; Catalog # NBP2-44431), to visualize intestinal goblet cells, and counterstained for E-Cadherin (red; Catalog # <a href="/p/antibodies/human-mouse-e-cadherin-antibody_af748" target="_self">AF748</a>) and DAPI (blue; Catalog # <a href="/p/fluorescent-probes-dyes/dapi_5748" target="_self">5748</a>). </p> </td> </tr> <tr class="row m___product-data-examples"> <td class="border-0"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand=""> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/bme001-05_cultrex-ultimatrix-reduced-growth-factor-basement-membrane-matrix-data-92202415929.jpg" data-srcset="https://resources.bio-techne.com/images/products/bme001-05_cultrex-ultimatrix-reduced-growth-factor-basement-membrane-matrix-data-92202415929.jpg" alt="A graph of endotoxin levels and protein concentrations for 20 recent lots of Cultrex UltiMatrix" title="Figure 2. Cultrex UltiMatrix shows improved lot-to-lot consistency with low endotoxin and high protein concentration across 20 recent lots of Cultrex UltiMatrix." data-caption="The brown lines represents the endotoxin levels measured in 20 recent lots of Cultrex UltiMatrix. The graph shows consistently low levels of endotoxin across the lots. Cultrex UltiMatrix is specifically designed to maintain low endotoxin levels. The blue lines represents the protein concentration measured in the same 20 lots of Cultrex UltiMatrix. The graph demonstrates the consistency of protein concentration from lot to lot, highlighting the product's ability to maintain higher, constant protein levels. Cultrex UltiMatrix is designed to provide consistent protein concentration for reliable experimental outcomes." data-badges="" data-application="N/A" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </td> <td class="w-75 border-0 align-top"> <p> <strong>Figure 2. Cultrex UltiMatrix shows improved lot-to-lot consistency with low endotoxin and high protein concentration across 20 recent lots of Cultrex UltiMatrix.</strong> The brown lines represents the endotoxin levels measured in 20 recent lots of Cultrex UltiMatrix. The graph shows consistently low levels of endotoxin across the lots. Cultrex UltiMatrix is specifically designed to maintain low endotoxin levels. The blue lines represents the protein concentration measured in the same 20 lots of Cultrex UltiMatrix. The graph demonstrates the consistency of protein concentration from lot to lot, highlighting the product's ability to maintain higher, constant protein levels. Cultrex UltiMatrix is designed to provide consistent protein concentration for reliable experimental outcomes. </p> </td> </tr> <tr class="row m___product-data-examples"> <td class="border-0"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand=""> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/bme001-05_cultrex-ultimatrix-reduced-growth-factor-basement-membrane-matrix-922024153039..png" data-srcset="https://resources.bio-techne.com/images/products/bme001-05_cultrex-ultimatrix-reduced-growth-factor-basement-membrane-matrix-922024153039..png" alt="" title="Figure 3. Cultrex UltiMatrix RGF Basement Membrane Extract (BME) Displays Consistent Lot-to-Lot ECM Composition, High Tensile Strength, and Gel Rates." data-caption="A) Simple Western analysis for Entactin, Laminin, and Collagen IV across two lots of both Cultrex UltiMatrix RGF BME (Catalog # BME001) and an alternate commercial BME matrix. Cultrex UltiMatrix RGF BME shows consistent expression of Entactin, compared to the alternate ECM matrix. B) The tensile strength (elastic modulus) dynamics across two lots of Cultrex UltiMatrix RGF BME and an alternate commercial BME matrix. The elastic modulus was measured at increasing temperatures to determine tensile strength and gelling rate across two independent lots of Cultrex UltiMatrix RGF BME (Catalog # BME001) and the alternate BME matrix. Cultrex UltiMatrix RGF BME was found to display consistent gelling rates and high tensile strength (storage modulus) compared to the alternate commercial BME matrix." data-badges="" data-application="N/A" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </td> <td class="w-75 border-0 align-top"> <p> <strong>Figure 3. Cultrex UltiMatrix RGF Basement Membrane Extract (BME) Displays Consistent Lot-to-Lot ECM Composition, High Tensile Strength, and Gel Rates.</strong> A) Simple Western analysis for Entactin, Laminin, and Collagen IV across two lots of both Cultrex UltiMatrix RGF BME (Catalog # BME001) and an alternate commercial BME matrix. Cultrex UltiMatrix RGF BME shows consistent expression of Entactin, compared to the alternate ECM matrix. B) The tensile strength (elastic modulus) dynamics across two lots of Cultrex UltiMatrix RGF BME and an alternate commercial BME matrix. The elastic modulus was measured at increasing temperatures to determine tensile strength and gelling rate across two independent lots of Cultrex UltiMatrix RGF BME (Catalog # BME001) and the alternate BME matrix. Cultrex UltiMatrix RGF BME was found to display consistent gelling rates and high tensile strength (storage modulus) compared to the alternate commercial BME matrix. </p> </td> </tr> <tr class="row m___product-data-examples"> <td class="border-0"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand=""> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/bme001-05_cultrex-ultimatrix-reduced-growth-factor-basement-membrane-matrix-922024153428..jpg" data-srcset="https://resources.bio-techne.com/images/products/bme001-05_cultrex-ultimatrix-reduced-growth-factor-basement-membrane-matrix-922024153428..jpg" alt="Human Descending Colon Organoids Grown in Cultrex UltiMatrix RGF BME" title="Figure 4. Human Descending Colon Organoids Grown in Cultrex UltiMatrix RGF BME." data-caption="Adult stem cells isolated from human descending colon were embedded in Cultrex UltiMatrix RGF BME and cultured in growth medium for 30 days prior to imaging. <b>A)</b> Brightfield image of descending colon organoid showing tissue invagination and epithelial layer formation. <b>B, C)</b> Descending colon organoids were stained with Chromogranin A (green; Catalog # <a class="NoLineLink" href="/p/antibodies/human-chromogranin-a-antibody-948128_mab90981" target="_self">MAB90981</a>), to visualize intestinal enteroendocrine cells, and counterstained for E-Cadherin (red; Catalog # <a class="NoLineLink" href="/p/antibodies/human-mouse-e-cadherin-antibody_af748" target="_self">AF748</a>) and DAPI (blue; Catalog # <a class="NoLineLink" href="/p/fluorescent-probes-dyes/dapi_5748" target="_self">5748</a>)." data-badges="" data-application="N/A" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </td> <td class="w-75 border-0 align-top"> <p> <strong>Figure 4. Human Descending Colon Organoids Grown in Cultrex UltiMatrix RGF BME.</strong> Adult stem cells isolated from human descending colon were embedded in Cultrex UltiMatrix RGF BME and cultured in growth medium for 30 days prior to imaging. <b>A)</b> Brightfield image of descending colon organoid showing tissue invagination and epithelial layer formation. <b>B, C)</b> Descending colon organoids were stained with Chromogranin A (green; Catalog # <a href="/p/antibodies/human-chromogranin-a-antibody-948128_mab90981" target="_self">MAB90981</a>), to visualize intestinal enteroendocrine cells, and counterstained for E-Cadherin (red; Catalog # <a href="/p/antibodies/human-mouse-e-cadherin-antibody_af748" target="_self">AF748</a>) and DAPI (blue; Catalog # <a href="/p/fluorescent-probes-dyes/dapi_5748" target="_self">5748</a>). </p> </td> </tr> <tr class="row m___product-data-examples"> <td class="border-0"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand=""> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/bme001-05_cultrex-ultimatrix-reduced-growth-factor-basement-membrane-matrix-922024153735..jpg" data-srcset="https://resources.bio-techne.com/images/products/bme001-05_cultrex-ultimatrix-reduced-growth-factor-basement-membrane-matrix-922024153735..jpg" alt="Human Lung Organoids Grown in Cultrex UltiMatrix RGF BME." title="Figure 5. Human Lung Organoids Grown in Cultrex UltiMatrix RGF BME." data-caption="Adult stem cells isolated from human lung biopsy tissue were embedded in Cultrex UltiMatrix RGF BME and cultured in lung organoid expansion medium where they were able to differentiate and exhibit key markers for various lung cell types. <b>A)</b> Lung organoids stained with anti-Cytokeratin 5 (KRT5) (green; Catalog # NB110-56916) and with anti-p63/ TP73L (red; Catalog # <a class="NoLineLink" href="/p/antibodies/human-p63-tp73l-antibody_af1916" target="_self">AF1916</a>) to visualize basal cells. <b>B)</b> Lung organoids stained with anti-Podoplanin (PDPN) (green; Catalog # NB600-1015) to visualize alveolar type I cells. <b>C)</b> Quantification of lung organoid size across organoids grown in Cultrex UltiMatrix RGF BME from 4 different lots shows high performance consistency of the matrix in this application." data-badges="" data-application="N/A" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </td> <td class="w-75 border-0 align-top"> <p> <strong>Figure 5. Human Lung Organoids Grown in Cultrex UltiMatrix RGF BME.</strong> Adult stem cells isolated from human lung biopsy tissue were embedded in Cultrex UltiMatrix RGF BME and cultured in lung organoid expansion medium where they were able to differentiate and exhibit key markers for various lung cell types. <b>A)</b> Lung organoids stained with anti-Cytokeratin 5 (KRT5) (green; Catalog # NB110-56916) and with anti-p63/ TP73L (red; Catalog # <a href="/p/antibodies/human-p63-tp73l-antibody_af1916" target="_self">AF1916</a>) to visualize basal cells. <b>B)</b> Lung organoids stained with anti-Podoplanin (PDPN) (green; Catalog # NB600-1015) to visualize alveolar type I cells. <b>C)</b> Quantification of lung organoid size across organoids grown in Cultrex UltiMatrix RGF BME from 4 different lots shows high performance consistency of the matrix in this application. </p> </td> </tr> <tr class="row m___product-data-examples"> <td class="border-0"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand=""> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/bme001-05_cultrex-ultimatrix-reduced-growth-factor-basement-membrane-matrix-922024154546..jpg" data-srcset="https://resources.bio-techne.com/images/products/bme001-05_cultrex-ultimatrix-reduced-growth-factor-basement-membrane-matrix-922024154546..jpg" alt="Cultrex UltiMatrix BME iPSC Expansion Compared to Competitor" title="Figure 6. Robust Induced Pluripotent Stem Cell (iPSC) Expansion Compared to an Alternate BME Supplier." data-caption="<b>(A)</b> Human iPSCs were passaged in 6 well plates (seeded at 7.5x10<sup>5</sup> cells/well) using either Cultrex UltiMatrix RGF BME (purple) or an Alternate BME Supplier (grey) as a substrate. At each passage cells were harvested and counted by hemocytometer. Cultrex UltiMatrix BME showed robust iPSC expansion compared to the Alternate BME Supplier. <b>B)</b> iPSC cells grown on Cultrex UltiMatrix BME retained expression of the pluripotency marker SSEA-4, as labeled using a Fluorescein conjugated SSEA-4 antibody (Catalog # FAB1435)." data-badges="" data-application="N/A" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </td> <td class="w-75 border-0 align-top"> <p> <strong>Figure 6. Robust Induced Pluripotent Stem Cell (iPSC) Expansion Compared to an Alternate BME Supplier.</strong> <b>(A)</b> Human iPSCs were passaged in 6 well plates (seeded at 7.5x10<sup>5</sup> cells/well) using either Cultrex UltiMatrix RGF BME (purple) or an Alternate BME Supplier (grey) as a substrate. At each passage cells were harvested and counted by hemocytometer. Cultrex UltiMatrix BME showed robust iPSC expansion compared to the Alternate BME Supplier. <b>B)</b> iPSC cells grown on Cultrex UltiMatrix BME retained expression of the pluripotency marker SSEA-4, as labeled using a Fluorescein conjugated SSEA-4 antibody (Catalog # FAB1435). </p> </td> </tr> <tr class="row m___product-data-examples"> <td class="border-0"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand=""> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/bme001-05_cultrex-ultimatrix-reduced-growth-factor-basement-membrane-matrix-922024154913..jpg" data-srcset="https://resources.bio-techne.com/images/products/bme001-05_cultrex-ultimatrix-reduced-growth-factor-basement-membrane-matrix-922024154913..jpg" alt="Improved Spheroid (Acinar) Maturation and Consistency Compared to Other Commercial Matrices" title="Figure 7. Improved Spheroid (Acinar) Maturation and Consistency Compared to Other Commercial Matrices." data-caption="Single MCF10A cells were cultured for in Cultrex UltiMatrix BME, Cultrex BME, Type R1 (3433-005-R1), or an alternate BME supplier. Over 12 days in culture, cells differentiated into luminal structures called “acini” and were quantified for acinar area. Cultrex UltiMatrix BME showed increased acinar maturation (larger area) compared to Cultrex BME, Type R1 and more consistent acinar area compared to the alternate BME supplier." data-badges="" data-application="N/A" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </td> <td class="w-75 border-0 align-top"> <p> <strong>Figure 7. Improved Spheroid (Acinar) Maturation and Consistency Compared to Other Commercial Matrices.</strong> Single MCF10A cells were cultured for in Cultrex UltiMatrix BME, Cultrex BME, Type R1 (3433-005-R1), or an alternate BME supplier. Over 12 days in culture, cells differentiated into luminal structures called “acini” and were quantified for acinar area. Cultrex UltiMatrix BME showed increased acinar maturation (larger area) compared to Cultrex BME, Type R1 and more consistent acinar area compared to the alternate BME supplier. </p> </td> </tr> <tr class="row m___product-data-examples"> <td class="border-0"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand=""> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.bio-techne.com/images/products/bme001-05_cultrex-ultimatrix-reduced-growth-factor-basement-membrane-matrix-922024155550..jpg" data-srcset="https://resources.bio-techne.com/images/products/bme001-05_cultrex-ultimatrix-reduced-growth-factor-basement-membrane-matrix-922024155550..jpg" alt="Cultrex UltiMatrix RGF BME has Optimized Extracellular Matrix Composition and Soluble Protein" title="Figure 8. Optimized Extracellular Matrix Composition and Soluble Protein." data-caption="<b>A)</b> Multiple lots of Cultrex UltiMatrix RGF BME were run by Simple Western to determine extracellular matrix protein composition. The Western blots show the matrix to have high lot-to-lot consistency of protein composition. <b>B)</b> Cultrex UltiMatrix RGF BME showed a lower amount of aggregated protein in the matrix solution compared to Cultrex BME, Type R1. Lower protein aggregation indicates a higher amount of soluble protein in solution, which improves protein crosslinking and matrix integrity in culture." data-badges="" data-application="N/A" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </td> <td class="w-75 border-0 align-top"> <p> <strong>Figure 8. Optimized Extracellular Matrix Composition and Soluble Protein.</strong> <b>A)</b> Multiple lots of Cultrex UltiMatrix RGF BME were run by Simple Western to determine extracellular matrix protein composition. The Western blots show the matrix to have high lot-to-lot consistency of protein composition. <b>B)</b> Cultrex UltiMatrix RGF BME showed a lower amount of aggregated protein in the matrix solution compared to Cultrex BME, Type R1. Lower protein aggregation indicates a higher amount of soluble protein in solution, which improves protein crosslinking and matrix integrity in culture. </p> </td> </tr> </article> </table> </div> <div id="preparation--storage" class="nav-link px-0" data-anchor-label="Preparation & Storage"><div id="cc_prep_storage"><h3>Preparation & Storage</h3><table class="table-bordered table" id="prep_storage_wrapper"><tr class="product-data"> <td class="product-data-label font-weight-bold p-3 align-top text-md-right w-25">Stability</td> <td class="product-data-value p-3 px-4 text-break"> Product is stable for three years from date of manufacture when stored at ≤ -70 °C . See lot specific Certificate of Analysis for expiration date. </td> </tr> <tr class="product-data"> <td class="product-data-label font-weight-bold p-3 align-top text-md-right w-25">Shipping Conditions</td> <td class="product-data-value p-3 px-4 text-break"> The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label. </td> </tr> <tr class="product-data"> <td class="product-data-label font-weight-bold p-3 align-top text-md-right w-25">Storage</td> <td class="product-data-value p-3 px-4 text-break"> Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. </td> </tr> </table> </div> </div> <div id="product-datasheets-anchor" class="nav-link static-anchor p-0" data-anchor-label="Product Datasheets"></div> </div> </div> <div class="tab-pane fade mx-4 py-4 show" id="citations-reviews--tab" data-tab-label="Citations / Reviews (6)" role="tabpanel" aria-labelledby="citations-reviews--tab-tab"><div class="product_details_anchors mb-2"> <div class="nav mx-0 pt-4 pb-2 m___nav-anchors d-none d-lg-flex"> <li class="nav-item m-0"> <a class="nav-link jump-anchor active" href="#citations"> Citations </a> </li> <li class="nav-item m-0"> <a class="nav-link jump-anchor active" href="#reviews"> Reviews </a> </li> </div> </div> <div id="citations"> <section id="citations-tab-content-placeholder" class="tab-content-placeholder" data-id="citations" data-product_code="BME001-05"> <div class="spinner-border spinner-border-sm mt-3 ml-3" role="status"> <span class="sr-only">Loading...</span> 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Cultrex UltiMatrix Reduced Growth Factor Basement Membrane Extract (BME001-05) by R&D Systems, Part of Bio-Techne