Advanced Western Blotting Solutions for Cancer and Immuno-Oncology

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<div class="highlighted"> <aside class="container section clearfix" role="complementary"> <div> </div> </aside> </div> <div id="main" class="container-fluid container-wide "> <div class="row"> <div class="col-md-12"> <div> <div id="block-bio-techne-global-breadcrumbs" class="system_breadcrumb_block"> <nav role="navigation" aria-labelledby="system-breadcrumb"> <h2 id="system-breadcrumb" class="visually-hidden">Breadcrumb</h2> <ol> <li> <a href="/">Home</a> </li> <li> <a href="/resources">Resources</a> </li> <li> <a href="/resources/instrument-applications">ProteinSimple Instrument Applications</a> </li> <li> Advanced Western Blotting Solutions For Cancer and Immuno-Oncology </li> </ol> </nav> </div> </div> </div> </div> <div class="row"> <div class="col-md-12"> <main> <section class="section"> <div> <div id="block-bio-techne-global-content" class="system_main_block"> <article> <div> <div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h1>Advanced Western Blotting Solutions for Cancer & Immuno-Oncology</h1></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-1-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--75-25"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:3_1_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p>Accelerate your <a href="/research-areas/cancer-profiling">cancer </a>&<a href="/research-areas/immuno-oncology"> immuno-oncology</a> research. Our next-generation cancer research equipment allows you to uncover new views into the role of proteins in cancer and accelerate your ability to develop and analyze protein-based therapeutics. <a href="/instruments/simple-western">Simple Western™ </a>can help you reveal new insights and advance <a href="/research-areas/cancer-profiling">cancer </a>&<a href="/research-areas/immuno-oncology"> immuno-oncology</a> research through the study of proteins, their function and signaling, through reliable, quantitative, and automated workflows.</p></div> </div> <div class="field_block:paragraph:3_1_text_to_media:field_call_to_action_global"> <a href="/instruments/simple-western" class="btn btn-primary" type="link"> Learn About Simple Western </a> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:3_1_text_to_media:field_indv_media"> <div> <a href="https://www.bio-techne.com/instruments/simple-western" title="Learn About Simple Western" > <img src="https://resources.bio-techne.com/bio-techne-assets/images/instruments/instrument-obt-teaser-jess-700x420-v2.png" alt="Jess Instrument Simple Western" loading="lazy" > </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><h3>Table of Contents</h3> <figure class="responsive-figure-table"><table> <tbody> <tr> <td> <ul> <li><a href="#Simple Western Advances Cancer Research">Simple Western Advances Cancer Research</a></li> <li><a href="#Immune Checkpoints Tumor Microenvironment Low Abundant Proteins">Immune Checkpoints & Tumor Hypoxia</a></li> <li><a href="#Immune Checkpoints Tumor Microenvironment Low Abundant Proteins">Tumor Microenvironment</a></li> <li><a href="#Immune Checkpoints Tumor Microenvironment Low Abundant Proteins">Biomarkers & Low Abundant Proteins</a></li> </ul> </td> <td> <ul> <li><a href="#From Your Peers in Cancer Research">From Your Peers in Cancer Research</a></li> <li><a href="#Quantitative Cell Signaling Analysis">Quantitative Cell Signaling Analysis</a></li> <li><a href="#Heterogeneity Analysis with Single-Cell Westerns">Heterogeneity Analysis with Single-Cell Westerns</a></li> <li><a href="#Related Resources">Related Resources in Cancer & Immuno-Oncology Research</a></li> </ul> </td> </tr> </tbody> </table></figure> <h2><a name="Simple Western Advances Cancer Research"></a>Simple Western Advances Cancer Research</h2></div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <div class="row card_row"> <div class="col-md-4"> <div class="h-100"> <div class="d-flex flex-column h-100"> <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: #00263A; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/icons/dark-jess-icon.png" alt="Simple Western Jess Icon" loading="lazy" > </div> </div> </div> <h3 class="card_title_htag"> <div><h3>Simple Western</h3></div> </h3> <div class="card_description flex-grow-1"> <div><p><a href="/instruments/simple-western">Simple Western™ systems</a> provide fully automated, hands-free Western blotting analysis, giving you highly quantitative and reproducible results, and using less sample in the process.</p></div> </div> <div class="card-bottom-link"></div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="d-flex flex-column h-100"> <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: #00263A; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/icons/data-comparison-icon.png" alt="Data Comparison icon" loading="lazy" > </div> </div> </div> <h3 class="card_title_htag"> <div>Quantification</div> </h3> <div class="card_description flex-grow-1"> <div><p>Simple Western provides fully quantitative results and has a wider dynamic range than a traditional Western blot.</p></div> </div> <div class="card-bottom-link"></div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="d-flex flex-column h-100"> <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: #00263A; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/icons/dark-graph-3-icon.png" alt="Consistency Graph Icon" loading="lazy" > </div> </div> </div> <h3 class="card_title_htag"> <div><h3>Reproducibility</h3></div> </h3> <div class="card_description flex-grow-1"> <div><p>Simple Western eliminates the unavoidable variation of<a href="/applications/western-blotting"> traditional Western blotting</a>, producing intra-assay CV’s <15%.</p></div> </div> <div class="card-bottom-link"></div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="d-flex flex-column h-100"> <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: #00263A; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/icons/dark-examine-icon.png" alt="Examine Icon" loading="lazy" > </div> </div> </div> <h3 class="card_title_htag"> <div><h3>Limited Sample</h3></div> </h3> <div class="card_description flex-grow-1"> <div><p>Quantitation of protein expression via Simple Western only requires input volumes of 3 µL, so the majority of your sample remains available for other characterization methods.</p></div> </div> <div class="card-bottom-link"></div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="d-flex flex-column h-100"> <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: #00263A; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/icons/dark-examine-data-icon.png" alt="Sensitivity Icon" loading="lazy" > </div> </div> </div> <h3 class="card_title_htag"> <div><h3>Sensitivity</h3></div> </h3> <div class="card_description flex-grow-1"> <div><p>Simple Western has chemiluminescence and <a href="/simple-western/stellar-nir-ir-fluorescence-detection-modules">industry-leading NIR/IR detection</a> that is 100X more sensitive than the closest competitive traditional Western blot fluorescence imaging system.</p></div> </div> <div class="card-bottom-link"></div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="d-flex flex-column h-100"> <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: #00263A; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/icons/dark-stopwatch-icon.png" alt="Stopwatch Icon" loading="lazy" > </div> </div> </div> <h3 class="card_title_htag"> <div><h3>Speed</h3></div> </h3> <div class="card_description flex-grow-1"> <div><p>Simple Western tests up to 25 samples in 3 hours or 96 samples overnight and allows for the <a href="/instruments/simple-western/multiplex-protein-analysis-simple-western">multiplex </a>detection of multiple proteins per sample.</p></div> </div> <div class="card-bottom-link"></div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--individual-button mb-4 paragraph--view-mode--default w-100"> <div class="row"> <div class="col"> <a href="/p/instruments/simple-western/request-quote" class="btn btn-primary float-left" type="link" > Request Simple Western Pricing </a> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="From Your Peers in Cancer Research"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <h2>From Your Peers in Cancer Research</h2> <div class="row card_row"> <div class="col-md-6"> <div class="h-100"> <div class="d-flex flex-column h-100"> <a href="/resources/testimonials/simple-western-wes-gets-fast-answers-for-cancer-research" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-jane.png" alt="Dr Jane Gray Thumbnail" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/resources/testimonials/simple-western-wes-gets-fast-answers-for-cancer-research" > <h3 class="card_title_htag"></h3> </a> <div class="card_description flex-grow-1"> <div><p><em>"[<a href="/instruments/simple-western">Simple Western</a>] is easy to use, convenient, and gives answers quickly. The throughput and system automation is a definite advantage."</em></p> <p>- Jane Gray, Ph.D., Head of Research Instrumentation, Cancer Research UK Cambridge</p></div> </div> <div class="card-bottom-link"> <div> <a href="/resources/testimonials/simple-western-wes-gets-fast-answers-for-cancer-research" > Fast Answers for Cancer Research </a> </div> </div> </div> </div> </div> <div class="col-md-6"> <div class="h-100"> <div class="d-flex flex-column h-100"> <a href="/resources/testimonials/simple-western-wes-pharmacodynamic-biomarker-discovery" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-krishna.png" alt="Krishna C. Yalla" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/resources/testimonials/simple-western-wes-pharmacodynamic-biomarker-discovery" > <h3 class="card_title_htag"></h3> </a> <div class="card_description flex-grow-1"> <div><p><em>"I was able to screen various <a href="/resources/simple-western-antibody-database">antibodies </a>in a single run within half a day. This had previously taken weeks and months using <a href="/applications/western-blotting">traditional Western blotting methods</a>."</em></p> <p><em>- </em>Krishna C. Yalla, Ph.D., Postdoctoral Research Scientist, Institute of Cancer Science</p></div> </div> <div class="card-bottom-link"> <div> <a href="/resources/testimonials/simple-western-wes-pharmacodynamic-biomarker-discovery" > Pharmacodynamic Biomarker Discovery </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="Immune Checkpoints Tumor Microenvironment Low Abundant Proteins"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <div class="row card_row"> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/literature/keeping-promise-of-immuno-oncology-simple-western-single-cell-western" > <div class="teaser-card-image-crop mb-3"> <img loading="lazy" class="card-background-image card-img-top" src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/cell-markers-teaser-card-background.png" /> </div> <div class="card-foreground-image"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/applications/dividing-cancer-cell-hero1.png" alt="3d rendering of cancer cells splitting" loading="lazy" > </div> </a> <a class="card_title_link" href="/resources/literature/keeping-promise-of-immuno-oncology-simple-western-single-cell-western" > <h3 class="card_title_htag"></h3> </a> <div class="card_description flex-grow-1"> <div><p>Simple Western offers highly specific, sensitive, and reproducible quantification of important immuno-oncology targets in human cells. These targets are detected simultaneously with <a href="/instruments/simple-western/simple-western-size-based-total-protein-assays">total protein</a> in your sample for accurate <a href="/instruments/simple-western/automate-total-protein-normalization-with-simple-western">total protein normalization</a>. Immune checkpoint proteins: PD1, PD-L1, CTLA-4, and TIGIT; <a href="/resources/webinars/exploiting-tumor-hypoxia-for-targeted-immunotherapy">Hypoxic </a>markers: HIF-1α, VEGFR-2, TGF-β, and ANG2; CAR T-Cell Target: CD19</p></div> </div> <div class="card-bottom-link"> <div> <a href="/resources/literature/keeping-promise-of-immuno-oncology-simple-western-single-cell-western" > Immune Checkpoints & Hypoxia </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/literature/wes-milo-synergize-profile-immune-cell-populations-tumor-microenvironment" > <div class="teaser-card-image-crop mb-3"> <img loading="lazy" class="card-background-image card-img-top" src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/cell-markers-teaser-card-background.png" /> </div> <div class="card-foreground-image"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/immuno-oncology-cancer-cell-500x300.png" alt="Immuno-oncology cancer cell" loading="lazy" > </div> </a> <a class="card_title_link" href="/resources/literature/wes-milo-synergize-profile-immune-cell-populations-tumor-microenvironment" > <h3 class="card_title_htag"></h3> </a> <div class="card_description flex-grow-1"> <div><p>Methodologies like flow cytometry have limitations such as off-target binding of variants or isoforms and difficulties in finding validated <a href="/resources/simple-western-antibody-database">antibodies</a>. <a href="/instruments/simple-western">Simple Western</a> and <a href="/instruments/single-cell-western">Single-Cell Western</a> systems partner to get you critical answers on the immune cell populations found in the tumor microenvironment, offering both population level views and single-cell level perspectives on cell subtypes.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/resources/literature/wes-milo-synergize-profile-immune-cell-populations-tumor-microenvironment" > Tumor Microenvironment </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/literature/breaking-laser-capture-microdissection-sample-size-road-blocks-simple-western" > <div class="teaser-card-image-crop mb-3"> <img loading="lazy" class="card-background-image card-img-top" src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/cell-markers-teaser-card-background.png" /> </div> <div class="card-foreground-image"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/bt-cancer-cell-hero-img-v2.png" alt="Cancer Cell Hero Image" loading="lazy" > </div> </a> <a class="card_title_link" href="/resources/literature/breaking-laser-capture-microdissection-sample-size-road-blocks-simple-western" > <h3 class="card_title_htag"></h3> </a> <div class="card_description flex-grow-1"> <div><p><a href="/instruments/simple-western">Simple Western</a> requires only a few microliters of precious samples like laser capture microdissection (LCM) sample to generate multiple data points. Simple Western also has high sensitivity multiplex detection of low abundance proteins that are difficult to detect by traditional Western blot. And it’s all wrapped up in a simple workflow that minimizes your hands-on time.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/resources/literature/breaking-laser-capture-microdissection-sample-size-road-blocks-simple-western" > Low Abundant Proteins </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><h2><a name="Quantitative Cell Signaling Analysis"></a>Quantitative Cell Signaling Analysis</h2></div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p>In <a href="/instruments/simple-western/simple-western-assays">Simple Western assays</a>, proteins are immobilized after separation, reducing variability and enabling quantitative measurements of protein abundance. <a href="/simple-western/stellar-nir-ir-fluorescence-detection-modules">High-sensitivity multiplexing</a> is easy using multiple detection channels or <a href="/instruments/simple-western/one-sample-two-western-immunoassay-replex-jess-abby" target="_blank">RePlex</a> to enable phospho/total measurements in the same capillary. Measure <a href="/instruments/simple-western/simple-western-size-based-total-protein-assays">total protein</a> in your sample for accurate <a href="/instruments/simple-western/automate-total-protein-normalization-with-simple-western">total protein normalization</a>.</p> <ul> <li>Virtual Poster Presentation <a href="/resources/literature/virtual-posters-proteinsimple#Novel%20Approach">“Novel approach for automated sequential immunoassay for quantitation and characterization of PI3K/AKT pathway proteins”</a> by Dr. Jessica Dermody</li> <li>Virtual Poster Presentation <a href="/resources/literature/virtual-posters-proteinsimple#Determination%20of%20Antibody">“Determination of antibody’s specificity towards phosphorylated protein targets with automated in-capillary enzyme treatment and immunoassay”</a> by Dr. Daryl Taketa</li> <li>Virtual Poster Presentation <a href="/resources/literature/virtual-posters-proteinsimple#Multiplexed%20Protein">“Multiplexed Protein and RNA Quantification on a Single Instrument Harmonizes Multi-omic Analyses of Biomarkers for Immunotherapies and Targeted Therapies in Non-Small Cell Lung Cancer”</a> by Dr. Chris Heger</li> </ul></div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_call_to_action_global"> <a href="/resources/instrument-applications/advanced-western-blotting-solutions-for-cell-signaling" class="btn btn-primary" type="link"> Cell Signaling & Signal Pathway Analysis </a> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_indv_media"> <div> <a href="https://www.bio-techne.com/resources/instrument-applications/advanced-western-blotting-solutions-for-cell-signaling" title="Cell Signaling & Signal Pathway Analysis" > <img src="https://resources.bio-techne.com/bio-techne-assets/images/instruments/jess-replex-akt-tpn.png" alt="RePlex on Jess quantifies phospho and pan AKT expression with total protein normalization" loading="lazy" > </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><h2><strong><a name="Heterogeneity Analysis with Single-Cell Westerns"></a>Heterogeneity Analysis with Single-Cell Westerns</strong></h2></div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--_-1-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--75-25"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:3_1_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p><a href="/instruments/single-cell-western">Single-Cell Western</a> on <a href="/p/single-cell-western/milo_p100">Milo™</a> adds single cell resolution and <a href="/resources/literature/reveal-cell-subtypes-protein-isoforms-phospho-protein-heterogeneity-milo">heterogeneity information</a> to cell signaling studies traditionally done with conventional Western blotting and <a href="/resources/instrument-applications/flow-cytometry-and-facs-cell-sorting-milo">simplifies challenging phospho-flow assays done with flow cytometry</a>.</p></div> </div> <div class="field_block:paragraph:3_1_text_to_media:field_call_to_action_global"> <a href="/instruments/single-cell-western" class="btn btn-primary" type="link"> Learn About Single-Cell Western </a> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:3_1_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/support/milo-thumnail.png" alt="o Track cell differentiation by measuring edited gene and downstream markers at the single cell level with Milo" loading="lazy" > </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_header_2_global"> <div><h3>Milo Adds Single-Cell Resolution and Heterogeneity Information</h3></div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p>The <a href="/instruments/single-cell-western">Milo Single-Cell Western system</a> helps detect discrete cell signaling states by measuring multiple phospho-proteins in individual cells within a population. Because cells are lysed in Single-Cell Westerns, membrane permeabilization is not required and both intracellular and intranuclear proteins are accessible for <a href="/resources/single-cell-western-antibody-database">antibody </a>probing, making it possible to use phospho-<a href="/resources/single-cell-western-antibody-database">antibodies </a>validated for Western blotting and simplifying some challenging intracellular flow assays.</p> <ul> <li> <p><a href="/resources/literature/reveal-cell-subtypes-protein-isoforms-phospho-protein-heterogeneity-milo">Single-Cell Signaling Studies</a></p> </li> </ul></div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/research-area-bioprocessing-cell-heterogeneity-750x400.jpg" alt="Bioprocessing Cell Heterogeneity Protein Expression with Milo" loading="lazy" > </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-1-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--75-25"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:3_1_text_to_media:field_header_2_global"> <div><h3>Tumor Landscape Mapping of Chemo-Resistant Cancer</h3></div> </div> <div class="field_block:paragraph:3_1_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p>In this webinar, Dr. Kyeung Min Joo, from Sungkyunkwan University School of Medicine, Seoul, South Korea describes their findings from the use of single-cell analysis technologies including<a href="/instruments/single-cell-western"> Single-Cell Westerns </a>to depict the tumor landscape of a single case of chemo-resistant metastatic, muscle-invasive urothelial bladder cancer (MIUBC).</p> <ul> <li> <p><a href="/resources/webinars/milo-single-cell-western-technology-and-single-cell-analysis-of-cancer">Single-Cell Analysis Webinar</a></p> </li> </ul></div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:3_1_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/webinars/io-webinar-video.png" alt="Immuno-oncology" loading="lazy" > </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_header_2_global"> <div><h3>Epigenetic Alterations in Single Nuclei</h3></div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p>Epigenetic alterations play important roles to drive the <a href="/research-areas/cancer-profiling">cancer</a> phenotype. Use Single-Cell Westerns to investigate histone modifications in single nuclei and understand epigenetic regulation of gene expression at the single-cell level.</p> <ul> <li> <p><a href="/resources/literature/adapting-single-cell-western-protocol-detect-histone-modifications">Epigenetic Modification App Note</a></p> </li> </ul></div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_call_to_action_global"> <a href="/p/instruments/single-cell-western/request-quote?productName=Milo&catalogNumber=P100" class="btn btn-primary" type="link"> Request Single-Cell Western Pricing </a> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/applications/cancer-io-histone.png" alt="Cancer histone graph" loading="lazy" > </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><h2><a name="Related Resources"></a></h2> <h2> </h2></div> </div> </div> <div> <div class="paragraph paragraph--type--_-1-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--75-25"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:3_1_text_to_media:field_header_2_global"> <div><h2>High-Impact Peer-Reviewed Cancer Research</h2></div> </div> <div class="field_block:paragraph:3_1_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p>Simple Western systems have been involved in breakthrough immunotherapies, including those involving immune checkpoint inhibitors,<a href="/resources/instrument-citations?research-area=cell%20therapy"> CAR T-cell therapy</a>, bispecific antibodies, and macrophage switching. <a href="/instruments/single-cell-western">Single-Cell Westerns</a> provide insight into population heterogeneity at the protein level and has been used to study the molecular underpinnings of malignant cell transformation with unprecedented detail.</p> <ul> <li><a href="/resources/literature/cutting-edge-immuno-oncology-simple-western-single-cell-western">View Immuno-Oncology Publications</a></li> </ul></div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:3_1_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/applications/nature-medicine-logo.svg" alt="Nature Medicine Logo" loading="lazy" > </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-1-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--75-25"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:3_1_text_to_media:field_header_2_global"> <div><h2>Related Resources in Cancer & Immuno-Oncology</h2></div> </div> <div class="field_block:paragraph:3_1_text_to_media:field_lg_sngl_frmtd_text_global"> <div><figure class="responsive-figure-table"><table> <tbody> <tr> <td> <ul> <li><a href="/research-areas/cancer-profiling">Cancer Biomarker Research</a></li> <li><a href="/research-areas/immuno-oncology/immuno-oncology-resources">Immuno-Oncology Resources</a></li> </ul> </td> <td> <ul> <li><a href="/resources/ebook/immuno-oncology" target="_blank">eBook: Foundations of Immuno-Oncology</a></li> <li><a href="/resources/literature/t-cell-therapies-ebook">eBook: T Cell-Based Therapies</a></li> </ul> </td> </tr> </tbody> </table></figure></div> </div> <div class="field_block:paragraph:3_1_text_to_media:field_call_to_action_global"> <a 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Jess’s ability to detect multiple proteins per sample further saves time compared to conventional Western blot." </div> </div> </div> </div> </div> <div class="carousel-item">  <div class="paragraph paragraph--type--individual-testimonial individual-testimonial paragraph--view-mode--default"> <div class="testimonial-info-group"> <div class="row"> <div class="col-lg-3 offset-lg-1 col-sm-12"> <div class="text-center text-lg-left"> <div class="individual-testimonial__headshot"> <div class="blazy blazy--field blazy--field-indv-img blazy--field-indv-img--default" data-blazy=""> <div data-b-token="b-5c2b2f9e221" class="media media--blazy media--rendered media--resourcesbio-techne media--bundle--remote-image"><img src='https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-debbi-hicks.jpg' alt='Debbi Hicks Thumbnail'> </div> </div> </div> </div> </div> <div class="col-10 offset-1 col-sm-10 offset-sm-1 col-md-8 offset-md-2 col-lg-6 offset-lg-0"> <div class="testimonial-info"> <div class="individual-testimonial__info"> <span class="individual-testimonial__name"> Debbie Hicks, Ph.D., Faculty Fellow </span><br/> <span class="individual-testimonial__position"> Wolfson Childhood Cancer Research Centre, Newcastle </span><br/> </div> </div> </div> <div class="col-12 col-sm-12 col-md-12 col-lg-2 quote-column text-center"> <img class="quote" loading="lazy" src="/themes/custom/bio_techne_global/images/testimonials-quote.svg"/> </div> </div> </div> <div class="row"> <div class="col-10 offset-1 col-md-10 offset-md-1 col-sm-10"> <div class="individual-testimonial__testimonial"> “Some of the proteins we wanted to detect are low in abundance and it’s really hard to get a good amount of protein for traditional Western blot. The low protein concentrations required by [Simple Western] makes it easier to save precious samples ensuring protein detection.” </div> </div> </div> </div> </div> <div class="carousel-item">  <div class="paragraph paragraph--type--individual-testimonial individual-testimonial paragraph--view-mode--default"> <div class="testimonial-info-group"> <div class="row"> <div class="col-lg-3 offset-lg-1 col-sm-12"> <div class="text-center text-lg-left"> <div class="individual-testimonial__headshot"> <div class="blazy blazy--field blazy--field-indv-img blazy--field-indv-img--default" data-blazy=""> <div data-b-token="b-92cf659c099" class="media media--blazy media--rendered media--resourcesbio-techne media--bundle--remote-image"><img src='https://resources.bio-techne.com/bio-techne-assets/images/testimonials/single-cell-western-yi-zhong.png' alt='Yi Zhong Thumbnail'> </div> </div> </div> </div> </div> <div class="col-10 offset-1 col-sm-10 offset-sm-1 col-md-8 offset-md-2 col-lg-6 offset-lg-0"> <div class="testimonial-info"> <div class="individual-testimonial__info"> <span class="individual-testimonial__name"> Yi Zhong, M.D., Ph.D. </span><br/> <span class="individual-testimonial__position"> Research Associate, Memorial Sloan Kettering Cancer Center </span><br/> </div> </div> </div> <div class="col-12 col-sm-12 col-md-12 col-lg-2 quote-column text-center"> <img class="quote" loading="lazy" src="/themes/custom/bio_techne_global/images/testimonials-quote.svg"/> </div> </div> </div> <div class="row"> <div class="col-10 offset-1 col-md-10 offset-md-1 col-sm-10"> <div class="individual-testimonial__testimonial"> “Milo permits study of proteins at the single-cell level to understand the heterogeneity among metastatic cells within a population.”  </div> </div> </div> </div> </div> <div class="carousel-item">  <div class="paragraph paragraph--type--individual-testimonial individual-testimonial paragraph--view-mode--default"> <div class="testimonial-info-group"> <div class="row"> <div class="col-lg-3 offset-lg-1 col-sm-12"> <div class="text-center text-lg-left"> <div class="individual-testimonial__headshot"> <div class="blazy blazy--field blazy--field-indv-img blazy--field-indv-img--default" data-blazy=""> <div data-b-token="b-342a9017d22" class="media media--blazy media--rendered media--resourcesbio-techne media--bundle--remote-image"><img src='https://resources.bio-techne.com/bio-techne-assets/images/testimonials/single-cell-western-prashant.png' alt='Prashant Vijay Thakkar Thumbnail'> </div> </div> </div> </div> </div> <div class="col-10 offset-1 col-sm-10 offset-sm-1 col-md-8 offset-md-2 col-lg-6 offset-lg-0"> <div class="testimonial-info"> <div class="individual-testimonial__info"> <span class="individual-testimonial__name"> Prashant Vijay Thakkar, Ph.D. </span><br/> <span class="individual-testimonial__position"> Department of Medicine, Weill Cornell Medicine </span><br/> </div> </div> </div> <div class="col-12 col-sm-12 col-md-12 col-lg-2 quote-column text-center"> <img class="quote" loading="lazy" src="/themes/custom/bio_techne_global/images/testimonials-quote.svg"/> </div> </div> </div> <div class="row"> <div class="col-10 offset-1 col-md-10 offset-md-1 col-sm-10"> <div class="individual-testimonial__testimonial"> "I was looking at two different isoforms of a protein, and it was important to know if the cells were expressing just one versus the other or both in a given cell. That was tricky until Milo came. We’re also getting more relevant information because Milo allows us to look at expression of this protein in tissue biopsies."  </div> </div> </div> </div> </div> </div> </div> </div> <div class="row"> <div class="col-md-12"> <ol class="carousel-indicators"> <li data-target="#carouselIndicators" data-slide-to="0" class="active"> </li> <li data-target="#carouselIndicators" data-slide-to="1"> </li> <li data-target="#carouselIndicators" data-slide-to="2"> </li> <li data-target="#carouselIndicators" data-slide-to="3"> </li> </ol> </div> </div> <a class="carousel-control-prev" href="#carouselIndicators" role="button" data-slide="prev"> <span class="carousel-control-prev-icon" aria-hidden="true"></span> <span class="sr-only">Previous</span> </a> <a class="carousel-control-next" href="#carouselIndicators" role="button" data-slide="next"> <span class="carousel-control-next-icon" aria-hidden="true"></span> <span class="sr-only">Next</span> </a> </div> </div> </div> </div> </div> </div> <div> 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