CINXE.COM

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class="content"> <div id="node-5106" class="node node-product-categories clearfix" about="/products/superoxide-dismutase-assay-kits" typeof="sioc:Item foaf:Document"> <span property="dc:title" content="Superoxide Dismutase Assay Kits" class="rdf-meta element-hidden"></span><span property="sioc:num_replies" content="0" datatype="xsd:integer" class="rdf-meta element-hidden"></span> <div class="content"> <div class="field field-name-body field-type-text-with-summary field-label-hidden"><div class="field-items"><div class="field-item even" property="content:encoded"><p>The <a href="/products/high-throughput-superoxide-dismutase-assay-kit_7501-500-k">High Throughput Superoxide Dismutase Assay Kit</a> provides researchers a simple, reproducible, and fast tool for assaying Superoxide Dismutase (SOD) activity in cell and tissue extracts. SODs are metalloenzymes that catalyze the dismutation of the superoxide radical (O<sub>2</sub><sup>•-</sup>) into hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and molecular oxygen (O<sub>2</sub>), providing an important defense against oxidative damage. In the Superoxide Dismutase Assay, superoxide ions are generated from the conversion of xanthine and O<sub>2</sub> to uric acid and H<sub>2</sub>O<sub>2</sub> by Xanthine Oxidase (XOD). The superoxide anion then coverts a tetrazolium salt into a formazan dye. Addition of SOD to this reaction reduces superoxide ion levels, thereby lowering the rate of formazan dye formation. SOD activity in the experimental sample is measured as the percent inhibition of the rate of formazan dye formation.</p> <h3>SOD Inhibition Assay Mechanism</h3> <table align="center" cellpadding="3" cellspacing="0" class="border" width="570"> <tbody> <tr> <td><img src="//resources.rndsystems.com/images/site/sod_inhibition_assay_mechanism.jpg" /></td> </tr> <tr> <td><strong>XOD and SOD Antagonism in the Generation of Formazan Dye.</strong> The conversion of xanthine and O<sub>2</sub> to uric acid and H<sub>2</sub>O<sub>2</sub>by XOD generates superoxide radicals. The superoxide anions reduce a tetrazolium salt (nitroblue tetrazolium [NBT] or WST-1) to a colored formazan product (NBT-diformazan or WST-1 formazan) that absorbs light. SOD scavenges superoxide anions, thereby reducing the rate of formazan dye formation.</td> </tr> </tbody> </table> <h3><a href="/products/high-throughput-superoxide-dismutase-assay-kit_7501-500-k">HT Superoxide Dismutase Assay Kit</a> (Catalog # 7501-500-K)</h3> <p>This is a 96-well microplate-based assay that is designed for the high throughput analysis of SOD in mammalian tissue and cell lysates. This kit utilizes WST-1 to detect superoxide ions generated by the XOD reaction. The superoxide radicals convert WST-1 to WST-1 formazan. Absorbance is measured at 450 nm using a standard microplate reader.</p> <h3>Kit Contents</h3> <ul> <li>SOD</li> <li>SOD Reaction Buffer</li> <li>Xanthine Oxidase</li> <li>Xanthine Solution</li> <li>Triton<sup>®</sup> X-100</li> <li>WST-1 Reagent</li> <li>96-well Microplates</li> </ul> <p class="note">Triton is a registered trademark of Dow Chemical Corporation.</p> </div></div></div> </div> </div> </div> </div> <div id="block-et-relatedcontent-menu-et-relatedcontent-menu-block" class="block block-et-relatedcontent-menu"> <div class="content"> <div class="relatedcontent_links"><h3>Related Information</h3></div> <div class="row evenheight"><div class="col-md-6 col-xs-12"><a href="https://www.rndsystems.com/research-area/als-related-molecules" class="btn btn-senary btn-relatedinfo" itemprop="relatedLink">ALS Related Molecules</a></div><div class="col-md-6 col-xs-12"><a href="https://www.rndsystems.com/research-area/diabetic-peripheral-neuropathy" class="btn btn-senary btn-relatedinfo" itemprop="relatedLink">Diabetic Peripheral Neuropathy</a></div><div class="col-md-6 col-xs-12"><a href="https://www.rndsystems.com/resources/technical/free-radicals-and-oxidative-stress" class="btn btn-senary btn-relatedinfo" itemprop="relatedLink">Free Radicals and Oxidative Stress</a></div><div class="col-md-6 col-xs-12"><a href="https://www.rndsystems.com/resources/articles/reactive-oxygen-species-ros" class="btn btn-senary btn-relatedinfo" itemprop="relatedLink">Mini-review: Reactive Oxygen Species (ROS)</a></div><div class="col-md-6 col-xs-12"><a href="https://www.rndsystems.com/research-area/oxidative-stress" class="btn btn-senary btn-relatedinfo" itemprop="relatedLink">Oxidative Stress</a></div><div class="col-md-6 col-xs-12"><a href="https://www.rndsystems.com/research-area/redox-enzymes" class="btn btn-senary btn-relatedinfo" itemprop="relatedLink">Redox Enzymes</a></div></div> </div> </div> <div id="block-block-26" class="block block-block"> <div class="content">  <div class="embeddedServiceInvitation" id="snapins_invite" aria-live="assertive" role="dialog" aria-atomic="true" style="display: none;"> <div class="embeddedServiceInvitationHeader" aria-labelledby="snapins_titletext" aria-describedby="snapins_bodytext" style="height: 48px;"> <img id="embeddedServiceAvatar" /> <span class="embeddedServiceTitleText" id="snapins_titletext">Need help?</span> <button type="button" id="closeInvite" class="embeddedServiceCloseIcon" aria-label="Exit invitation">×</button> </div> <div class="embeddedServiceInvitationBody"> <p id="snapins_bodytext">How can we help you?</p> </div> <div class="embeddedServiceInvitationFooter" aria-describedby="snapins_bodytext"> <button type="button" class="embeddedServiceActionButton" id="rejectInvite">Close</button> <button type="button" class="embeddedServiceActionButton" id="acceptInvite">Start Chat</button> </div> </div> </div> </div> </div> </div> <div class="grid_3"> <div class="column_menu"> <a href="/resources/literature" class="parent">Literature</a><div class="right_menu_contents hide"><ul class="menu"><li class="first leaf"><a href="/resources/articles" title="" id="menu-literature-29336">Articles</a></li> <li class="leaf"><a href="/resources/literature" title="" id="menu-literature-29341">Literature</a></li> <li class="last leaf"><a href="/resources/posters" title="" id="menu-literature-29346">Posters</a></li> </ul></div><a href="/Products" class="parent">Product Categories</a><div class="right_menu_contents"><ul class="menu"><li class="first collapsed"><a href="/products/proteins" id="menu-products-12696">Recombinant Proteins</a></li> <li class="leaf"><a href="/products/good-manufacturing-practices-gmp-grade-proteins" id="menu-products-112931">GMP Proteins</a></li> <li class="leaf"><a href="/products/enzymes" id="menu-products-121111">Enzymes</a></li> <li class="leaf"><a href="/products/small-molecules-peptides" id="menu-products-112711">Small Molecules & Peptides</a></li> <li class="leaf"><a href="/products/immunotherapy" id="menu-products-121081">Immunotherapy</a></li> <li class="leaf"><a href="/products/elispot-kits-fluorospot-kits" id="menu-products-12681">ELISpots</a></li> <li class="leaf"><a href="/products/cdna-clones" title="R&D Systems offers large selections of cDNA Clones and expression-ready vectors. 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