Screening of Thyroid Stimulating Hormone Using Paper-Based Lateral Flow Device

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Koji Suzuki</a>, <a href="https://publications.waset.org/abstracts/search?q=Daniel%20Citterio"> Daniel Citterio</a>, <a href="https://publications.waset.org/abstracts/search?q=Orawon%20Chailapakul"> Orawon Chailapakul</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A paper-based lateral flow device for screening thyroid stimulating hormone (TSH) is reported. A sandwich immunoassay was performed using two mouse monoclonal TSH antibodies (anti-hTSH 5403 and 5404) as immobilized and labeled antibodies for capturing TSH samples. Test (anti-hTSH 5403) and control (goat anti-Mouse IgG) lines were fabricated on nitrocellulose membrane (NCM) using ballpoint pen printed with a speed of 3 cm/s and thickness setting of 1. The novel gold nanoparticles europium complex (AuNPs@Eu) was used as fluorescence label compared to conventional AuNPs label. The results obtained with this device can be visually assessed by the naked eyes and under UV hand lamps, and quantitative analysis can be performed using the ImageJ program. The limit of detection (LOD) under UV hand lamps (0.1 碌IU/mL) provided 50-fold greater sensitivity than AuNPs (5 碌IU/mL), which is suitable for both hypothyroidism and hyperthyroidism screening within 30 min. A linear relationship between the red intensity and the logarithmic concentrations of TSH was observed with a good correlation (R虏=0.992). Furthermore, the device can be effectively applied for screening TSH in the spiked human serum with recovery range of 96.80-104.45% and RSD of 2.18-3.63%. Therefore, the developed device is an alternative method for TSH screening which provides a lot of advantages including low cost, short time analysis, ease of use, disposability, portability, and on-site measurement. <iframe src="https://publications.waset.org/abstracts/67545.pdf" style="width:100%; height:400px;" frameborder="0"></iframe> <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=thyroid%20stimulating%20hormone" title="thyroid stimulating hormone">thyroid stimulating hormone</a>, <a href="https://publications.waset.org/abstracts/search?q=paper-based%20lateral%20flow" title=" paper-based lateral flow"> paper-based lateral flow</a>, <a href="https://publications.waset.org/abstracts/search?q=hypothyroidism" title=" hypothyroidism"> hypothyroidism</a>, <a href="https://publications.waset.org/abstracts/search?q=hyperthyroidism" title=" hyperthyroidism"> hyperthyroidism</a> </p> <a href="https://publications.waset.org/abstracts/67545/screening-of-thyroid-stimulating-hormone-using-paper-based-lateral-flow-device" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/67545.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">372</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates its 16th foundational anniversary</a></li> </ul> </div> <div 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Screening of Thyroid Stimulating Hormone Using Paper-Based Lateral Flow Device