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></span> <span aria-label="breadcrumb separator">/</span> <span class="breadcrumb-current-title">Efficient Separation and Retention for Diverse Peptide Analysis Challenges</span></div></div></header><div class="site-inner"><div class="content-sidebar-wrap"><main class="content"><article class="post-17975 post type-post status-publish format-standard has-post-thumbnail category-biopharmaceutical category-mam category-uhplc tag-biopharmaceutical tag-mam tag-peptide-analysis tag-uhplc entry" aria-label="Efficient Separation and Retention for Diverse Peptide Analysis Challenges"><header class="entry-header"><h1 class="entry-title">Efficient Separation and Retention for Diverse Peptide Analysis Challenges</h1> <span class="post-info-author"> By <a href="https://www.thermofisher.com/blog/analyteguru/tf-author/palwasha-habibi-marketing-specialist-thermo-fisher-scientific/" title="Posts by Palwasha Habibi" rel="author">Palwasha Habibi, Marketing Specialist, High Performance Chromatography Solutions, Thermo Fisher Scientific</a> </span> <span class="post-info-date"> 08.12.2024 </span> </header><div class="entry-content"> <p>Peptide analysis often involves complex samples matrices, such as biological fluids or tissue extracts, which may contain a wide range of peptides with varying concentrations. Analyzing and identifying specific peptides of interest within this complexity can be challenging. </p> <p>Peptide mapping analysis plays an important role in the characterization of biotherapeutics, enabling the confirmation of primary sequences and the identification of post-translational modifications (PTMs), such as phosphorylation or glycosylation. These modifications can significantly impact peptide behavior and complicate analysis.  </p> <figure class="wp-block-image size-large"><img decoding="async" width="1024" height="576" src="https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2024/08/081224-Hypersil-1024x576.jpg" alt="" class="wp-image-17976" srcset="https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2024/08/081224-Hypersil-1024x576.jpg 1024w, https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2024/08/081224-Hypersil-300x169.jpg 300w, https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2024/08/081224-Hypersil-768x432.jpg 768w, https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2024/08/081224-Hypersil.jpg 1200w" sizes="(max-width: 1024px) 100vw, 1024px" /></figure> <p>You will need a chromatography column with excellent chromatographic performance that can retain both hydrophobic and hydrophilic peptides, like<strong> </strong>our new <a href="https://www.thermofisher.com/order/catalog/product/26002-152130?SID=srch-srp-26002-152130">Hypersil GOLD Peptide column</a>.</p> <p>Advantages of this peptide column include:</p> <ul class="wp-block-list"> <li>Unique bonded phase chemistry – a proprietary C18 bonded phase with a high surface area coverage, which provides a balance between hydrophobic and hydrophilic interactions. This allows for efficient separation and retention of a wide range of peptides, regardless of hydrophobicity or hydrophilicity.</li> </ul> <ul class="wp-block-list"> <li>High purity silica – the narrow particle size distribution offers unmatched performance in peptide analysis. </li> </ul> <p><strong>What specialized techniques and expertise are required to identify and characterize PTMs in peptide analysis? </strong> </p> <p>Monitoring of PTMs requires careful consideration of several key factors, including reproducibility, sensitivity, precision, and throughput. Hypersil GOLD Peptide columns provide outstanding performance and exceptional durability, making them the ideal choice for your peptide monitoring needs.</p> <p><strong>Hypersil GOLD Peptide column performance is assured through:</strong></p> <ul class="wp-block-list"> <li>Dual quality confirmation, including a peptide-based Quality Control (QC) test to deliver confidence in ultrapure silica, ligand bonding, and column packing for unparalleled sequence coverage and higher peak capacity compared to other peptide columns.</li> </ul> <ul class="wp-block-list"> <li>Consistent and reliable results – chromatographically tested during QC in the manufacturing process to ensure column-to-column and lot-to-lot reproducibility.  </li> </ul> <p><strong>How can you achieve greater sensitivity and lower limits of quantitation (LOQs) in peptide analysis with the Hypersil GOLD Peptide columns?</strong></p> <ul class="wp-block-list"> <li>1.9 µm particle size produces sharp symmetric peak shape and peak area with higher efficiency, greater sensitivity and LOQs.</li> <li>Optimized peptide retention- strong retention time reproducibility, retained, and separated hydrophobic and hydrophilic peptides.</li> <li>Precise chromatographic resolution values for deamidated species in the PENNYK region. </li> </ul> <figure class="wp-block-image size-large"><img decoding="async" width="1024" height="717" src="https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2024/08/081224-Figure-4-blog-1024x717.png" alt="" class="wp-image-17977" srcset="https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2024/08/081224-Figure-4-blog-1024x717.png 1024w, https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2024/08/081224-Figure-4-blog-300x210.png 300w, https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2024/08/081224-Figure-4-blog-768x538.png 768w, https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2024/08/081224-Figure-4-blog-1536x1075.png 1536w, https://www.thermofisher.com/blog/analyteguru/wp-content/uploads/sites/25/2024/08/081224-Figure-4-blog.png 2000w" sizes="(max-width: 1024px) 100vw, 1024px" /><figcaption class="wp-element-caption"><em><a href="https://assets.thermofisher.com/TFS-Assets/CMD/Application-Notes/an-002735-ccs-hypersil-gold-peptide-reversed-phase-c18-an002735-na-en.pdf" target="_blank" rel="noreferrer noopener">Figure 1. Extracted ion chromatograms for the Fc peptide GFYPSDIAVEWESNGQPENNYK on a Hypersil GOLD Peptide column</a>.</em></figcaption></figure> <p>You can read more about the benefits of using the Hypersil GOLD Peptide column for your peptide analysis in this <a href="https://assets.thermofisher.com/TFS-Assets/CMD/Application-Notes/an-002735-ccs-hypersil-gold-peptide-reversed-phase-c18-an002735-na-en.pdf" target="_blank" rel="noreferrer noopener">application note</a> and this <a href="https://www.thermofisher.com/document-connect/document-connect.html?url=https://assets.thermofisher.com/TFS-Assets%2FCMD%2FFlyers%2Ffl-002823-ccs-lc-column-hypersil-gold-peptides-fl002823-na-en.pdf" target="_blank" rel="noreferrer noopener">flyer</a>.</p> <p>Visit our <a href="https://www.linkedin.com/showcase/chromatography-&-mass-spectrometry/posts/?feedView=all" target="_blank" rel="noreferrer noopener">LinkedIn page</a> #PeptideMapping #MAM #Biopharma</p> </div></article><section class="author-box"><h4 class="author-box-title">Palwasha Habibi</h4><div class="author-box-content" itemprop="description">Palwasha Habibi works in the Thermo Fisher Scientific Chromatography and Mass Spectrometry Division (CMD) as a marketing specialist in the High Performance Chromatography Solutions (HPCS) business unit. Palwasha has a bachelor’s in business and marketing and has been working professionally in the scientific industry as a marketer since 2018. Primary responsibilities include creating content to help customers choose the products they need for their analytical research.</div></section><div class="adjacent-entry-pagination pagination"><div class="pagination-previous alignleft"><i class="fa fa-icon fa-chevron-left"></i> <a href="https://www.thermofisher.com/blog/analyteguru/why-everyone-is-talking-about-pfas-and-epa-method-1621/" rel="prev">Why Everyone is Talking About PFAS and EPA Method 1621</a></div><div class="pagination-next alignright"><a href="https://www.thermofisher.com/blog/analyteguru/trouble-with-hilic-acn-how-to-stabilize-your-hplc-operations/" rel="next">Trouble With HILIC & ACN? How to Stabilize Your HPLC Operations</a> <i class="fa fa-icon fa-chevron-right"></i></div></div></main></div></div><footer class="site-footer"><div class="wrap"><div class="creds"><p class="footer-links"><a href="https://corporate.thermofisher.com/en/footer/privacy-statement.html" target="_blank" rel="noopener noreferrer">Privacy Statement</a><a href="https://corporate.thermofisher.com/en/footer/terms-and-conditions.html" target="_blank" rel="noopener noreferrer">Terms & Conditions</a><a href="/blog/locations/">Locations</a><a href="/blog/sitemap/">Sitemap</a></p><p>© 2024 <a href="https://www.thermofisher.com">Thermo Fisher Scientific</a>. 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