TY - JFULL AU - R. Sullivan and T. Holden and G. Tremberger and Jr and E. Cheung and C. Branch and J. Burrero and G. Surpris and S. Quintana and A. Rameau and N. Gadura and H. Yao and R. Subramaniam and P. Schneider and S. A. Rotenberg and P. Marchese and A. Flamhlolz and D. Lieberman and T. Cheung PY - 2008/9/ TI - Fractal Dimension of Breast Cancer Cell Migration in a Wound Healing Assay T2 - International Journal of Biomedical and Biological Engineering SP - 185 EP - 191 VL - 2 SN - 1307-6892 UR - https://publications.waset.org/pdf/4794 PU - World Academy of Science, Engineering and Technology NX - Open Science Index 20, 2008 N2 - Migration in breast cancer cell wound healing assay had been studied using image fractal dimension analysis. The migration of MDA-MB-231 cells (highly motile) in a wound healing assay was captured using time-lapse phase contrast video microscopy and compared to MDA-MB-468 cell migration (moderately motile). The Higuchi fractal method was used to compute the fractal dimension of the image intensity fluctuation along a single pixel width region parallel to the wound. The near-wound region fractal dimension was found to decrease three times faster in the MDA-MB- 231 cells initially as compared to the less cancerous MDA-MB-468 cells. The inner region fractal dimension was found to be fairly constant for both cell types in time and suggests a wound influence range of about 15 cell layer. The box-counting fractal dimension method was also used to study region of interest (ROI). The MDAMB- 468 ROI area fractal dimension was found to decrease continuously up to 7 hours. The MDA-MB-231 ROI area fractal dimension was found to increase and is consistent with the behavior of a HGF-treated MDA-MB-231 wound healing assay posted in the public domain. A fractal dimension based capacity index has been formulated to quantify the invasiveness of the MDA-MB-231 cells in the perpendicular-to-wound direction. Our results suggest that image intensity fluctuation fractal dimension analysis can be used as a tool to quantify cell migration in terms of cancer severity and treatment responses. ER -