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<meta name="description" content="Search results for: STAT"> <meta name="keywords" content="STAT"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none navbar-toggler ml-auto" type="button" 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class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="STAT"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 33</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: STAT</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">33</span> Induction of Apoptosis by Diosmin through Interleukins/STAT and Mitochondria Mediated Pathway in Hep-2 and KB Cells</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=M.%20Rajasekar">M. Rajasekar</a>, <a href="https://publications.waset.org/abstracts/search?q=K.%20Suresh"> K. Suresh</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Diosmin is a flavonoid, most abundantly found in many citrus fruits. As a flavonoid, it possesses a multitude of biological activities including anti-hyperglycemic, anti-lipid peroxidative, anti-inflammatory, antioxidant, and anti-mutagenic properties. At this point, we established the anti-proliferative and apoptosis-inducing activities of diosmin in Hep-2 and KB cells. Diosmin has cytotoxic effects through inhibiting cellular proliferation of Hep-2 and KB cells, which leads to the induction of apoptosis, as apparent by an increase in the fraction of cells in the sub-G1phase of the cell cycle. Results exposed that inhibition of cell proliferation is associated with regulation of the Interleukins/STAT pathway. In addition, Diosmin treatment with Hep-2 and KB cells actively stimulated reactive oxygen species (ROS) and mitochondrial membrane depolarization. And also an imbalance in the Bax/Bcl-2 ratio triggered the caspase cascade and shifting the balance in favor of apoptosis. These observations conclude that Diosmin induce apoptosis via Interleukins /STAT-mediated pathway. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=diosmin" title="diosmin">diosmin</a>, <a href="https://publications.waset.org/abstracts/search?q=apoptosis" title=" apoptosis"> apoptosis</a>, <a href="https://publications.waset.org/abstracts/search?q=antioxidant" title=" antioxidant"> antioxidant</a>, <a href="https://publications.waset.org/abstracts/search?q=STAT%20pathway" title=" STAT pathway"> STAT pathway</a> </p> <a href="https://publications.waset.org/abstracts/37353/induction-of-apoptosis-by-diosmin-through-interleukinsstat-and-mitochondria-mediated-pathway-in-hep-2-and-kb-cells" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/37353.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">328</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">32</span> Dissolution of Zeolite as a Sorbent in Flue Gas Desulphurization Process Using a pH Stat Apparatus</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Hilary%20Rutto">Hilary Rutto</a>, <a href="https://publications.waset.org/abstracts/search?q=John%20Kabuba"> John Kabuba</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Sulphur dioxide is a harmful gaseous product that needs to be minimized in the atmosphere. This research work investigates the use of zeolite as a possible additive that can improve the sulphur dioxide capture in wet flue gas desulphurisation dissolution process. This work determines the effect of temperature, solid to liquid ratio, acid concentration and stirring speed on the leaching of zeolite using a pH stat apparatus. The atomic absorption spectrometer was used to measure the calcium ions from the solution. It was found that the dissolution rate of zeolite decreased with increase in solid to liquid ratio and increases with increase in temperature, stirring speed and acid concentration. The activation energy for the dissolution rate of zeolite in hydrochloric acid was found to be 9.29kJ/mol. and therefore the product layer diffusion was the rate limiting step. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=calcium%20ion" title="calcium ion">calcium ion</a>, <a href="https://publications.waset.org/abstracts/search?q=pH%20stat%20apparatus" title=" pH stat apparatus"> pH stat apparatus</a>, <a href="https://publications.waset.org/abstracts/search?q=wet%20flue%20gas%20desulphurization" title=" wet flue gas desulphurization"> wet flue gas desulphurization</a>, <a href="https://publications.waset.org/abstracts/search?q=zeolite" title=" zeolite"> zeolite</a> </p> <a href="https://publications.waset.org/abstracts/13102/dissolution-of-zeolite-as-a-sorbent-in-flue-gas-desulphurization-process-using-a-ph-stat-apparatus" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/13102.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">284</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">31</span> Dissolution of South African Limestone for Wet Flue Gas Desulphurization</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Lawrence%20Koech">Lawrence Koech</a>, <a href="https://publications.waset.org/abstracts/search?q=Ray%20Everson"> Ray Everson</a>, <a href="https://publications.waset.org/abstracts/search?q=Hein%20Neomagus"> Hein Neomagus</a>, <a href="https://publications.waset.org/abstracts/search?q=Hilary%20Rutto"> Hilary Rutto </a> </p> <p class="card-text"><strong>Abstract:</strong></p> Wet Flue gas desulphurization (FGD) systems are commonly used to remove sulphur dioxide from flue gas by contacting it with limestone in aqueous phase which is obtained by dissolution. Dissolution is important as it affects the overall performance of a wet FGD system. In the present study, effects of pH, stirring speed, solid to liquid ratio and acid concentration on the dissolution of limestone using an organic acid (adipic acid) were investigated. This was investigated using the pH stat apparatus. Calcium ions were analyzed at the end of each experiment using Atomic Absorption (AAS) machine. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=desulphurization" title="desulphurization">desulphurization</a>, <a href="https://publications.waset.org/abstracts/search?q=limestone" title=" limestone"> limestone</a>, <a href="https://publications.waset.org/abstracts/search?q=dissolution" title=" dissolution"> dissolution</a>, <a href="https://publications.waset.org/abstracts/search?q=pH%20stat%20apparatus" title=" pH stat apparatus"> pH stat apparatus</a> </p> <a href="https://publications.waset.org/abstracts/18656/dissolution-of-south-african-limestone-for-wet-flue-gas-desulphurization" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/18656.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">461</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">30</span> The Immunology Evolutionary Relationship between Signal Transducer and Activator of Transcription Genes from Three Different Shrimp Species in Response to White Spot Syndrome Virus Infection</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=T.%20C.%20C.%20Soo">T. C. C. Soo</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20Bhassu"> S. Bhassu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Unlike the common presence of both innate and adaptive immunity in vertebrates, crustaceans, in particular, shrimps, have been discovered to possess only innate immunity. This further emphasizes the importance of innate immunity within shrimps in pathogenic resistance. Under the study of pathogenic immune challenge, different shrimp species actually exhibit varying degrees of immune resistance towards the same pathogen. Furthermore, even within the same shrimp species, different batches of challenged shrimps can have different strengths of immune defence. Several important pathways are activated within shrimps during pathogenic infection. One of them is JAK-STAT pathway that is activated during bacterial, viral and fungal infections by which STAT(Signal Transducer and Activator of Transcription) gene is the core element of the pathway. Based on theory of Central Dogma, the genomic information is transmitted in the order of DNA, RNA and protein. This study is focused in uncovering the important evolutionary patterns present within the DNA (non-coding region) and RNA (coding region). The three shrimp species involved are Macrobrachium rosenbergii, Penaeus monodon and Litopenaeus vannamei which all possess commercial significance. The shrimp species were challenged with a famous penaeid shrimp virus called white spot syndrome virus (WSSV) which can cause serious lethality. Tissue samples were collected during time intervals of 0h, 3h, 6h, 12h, 24h, 36h and 48h. The DNA and RNA samples were then extracted using conventional kits from the hepatopancreas tissue samples. PCR technique together with designed STAT gene conserved primers were utilized for identification of the STAT coding sequences using RNA-converted cDNA samples and subsequent characterization using various bioinformatics approaches including Ramachandran plot, ProtParam and SWISS-MODEL. The varying levels of immune STAT gene activation for the three shrimp species during WSSV infection were confirmed using qRT-PCR technique. For one sample, three biological replicates with three technical replicates each were used for qRT-PCR. On the other hand, DNA samples were important for uncovering the structural variations within the genomic region of STAT gene which would greatly assist in understanding the STAT protein functional variations. The partially-overlapping primers technique was used for the genomic region sequencing. The evolutionary inferences and event predictions were then conducted through the Bayesian Inference method using all the acquired coding and non-coding sequences. This was supplemented by the construction of conventional phylogenetic trees using Maximum likelihood method. The results showed that adaptive evolution caused STAT gene sequence mutations between different shrimp species which led to evolutionary divergence event. Subsequently, the divergent sites were correlated to the differing expressions of STAT gene. Ultimately, this study assists in knowing the shrimp species innate immune variability and selection of disease resistant shrimps for breeding purpose. The deeper understanding of STAT gene evolution from the perspective of both purifying and adaptive approaches not only can provide better immunological insight among shrimp species, but also can be used as a good reference for immunological studies in humans or other model organisms. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=gene%20evolution" title="gene evolution">gene evolution</a>, <a href="https://publications.waset.org/abstracts/search?q=JAK-STAT%20pathway" title=" JAK-STAT pathway"> JAK-STAT pathway</a>, <a href="https://publications.waset.org/abstracts/search?q=immunology" title=" immunology"> immunology</a>, <a href="https://publications.waset.org/abstracts/search?q=STAT%20gene" title=" STAT gene"> STAT gene</a> </p> <a href="https://publications.waset.org/abstracts/87267/the-immunology-evolutionary-relationship-between-signal-transducer-and-activator-of-transcription-genes-from-three-different-shrimp-species-in-response-to-white-spot-syndrome-virus-infection" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/87267.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">150</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">29</span> Impact of Crises on Official Statistics: Environmental Statistics at Statistical Centre for the Cooperation Council for the Arab Countries of the Gulf during the COVID-19 Pandemic: A Case Study</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ibtihaj%20Al-Siyabi">Ibtihaj Al-Siyabi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The crisis of COVID-19 posed enormous challenges to the statistical providers. While official statistics were disrupted by the pandemic and related containment measures, there was a growing and pressing need for real-time data and statistics to inform decisions. This paper gives an account of the way the pandemic impacted the operations of the National Statistical Offices (NSOs) in general in terms of data collection and methods used and the main challenges encountered by them based on international surveys. It highlights the performance of the Statistical Centre for the Cooperation Council for the Arab Countries of the Gulf, GCC-STAT, and its responsiveness to the pandemic placing special emphasis on environmental statistics. The paper concludes by confirming the GCC-STAT’s resilience and success in facing the challenges. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=NSO" title="NSO">NSO</a>, <a href="https://publications.waset.org/abstracts/search?q=COVID-19" title=" COVID-19"> COVID-19</a>, <a href="https://publications.waset.org/abstracts/search?q=statistics" title=" statistics"> statistics</a>, <a href="https://publications.waset.org/abstracts/search?q=crisis" title=" crisis"> crisis</a>, <a href="https://publications.waset.org/abstracts/search?q=pandemic" title=" pandemic"> pandemic</a> </p> <a href="https://publications.waset.org/abstracts/151995/impact-of-crises-on-official-statistics-environmental-statistics-at-statistical-centre-for-the-cooperation-council-for-the-arab-countries-of-the-gulf-during-the-covid-19-pandemic-a-case-study" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/151995.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">140</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">28</span> Leaching of Copper from Copper Ore Using Sulphuric Acid in the Presence of Hydrogen Peroxide as an Oxidizing Agent: An Optimized Process</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Hilary%20Rutto">Hilary Rutto</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Leaching with acids are the most commonly reagents used to remove copper ions from its copper ores. It is important that the process conditions are optimized to improve the leaching efficiency. In the present study the effects of pH, oxidizing agent (hydrogen peroxide), stirring speed, solid to liquid ratio and acid concentration on the leaching of copper ions from it ore were investigated using a pH Stat apparatus. Copper ions were analyzed at the end of each experiment using Atomic Absorption (AAS) machine. Results showed that leaching efficiency improved with an increase in acid concentration, stirring speed, oxidizing agent, pH and decreased with an increase in the solid to liquid ratio. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=leaching" title="leaching">leaching</a>, <a href="https://publications.waset.org/abstracts/search?q=copper" title=" copper"> copper</a>, <a href="https://publications.waset.org/abstracts/search?q=oxidizing%20agent" title=" oxidizing agent"> oxidizing agent</a>, <a href="https://publications.waset.org/abstracts/search?q=pH%20stat%20apparatus" title=" pH stat apparatus"> pH stat apparatus</a> </p> <a href="https://publications.waset.org/abstracts/22113/leaching-of-copper-from-copper-ore-using-sulphuric-acid-in-the-presence-of-hydrogen-peroxide-as-an-oxidizing-agent-an-optimized-process" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/22113.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">377</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">27</span> Reactivity Study on South African Calcium Based Material Using a pH-Stat and Citric Acid: A Statistical Approach</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Hilary%20Rutto">Hilary Rutto</a>, <a href="https://publications.waset.org/abstracts/search?q=Mbali%20Chiliza"> Mbali Chiliza</a>, <a href="https://publications.waset.org/abstracts/search?q=Tumisang%20Seodigeng"> Tumisang Seodigeng</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The study on reactivity of calcined calcium-based material is very important in dry flue gas desulphurisation (FGD) process, so as to produce absorbent with high sulphur dioxide capture capacity during the hydration process. The effect of calcining temperature and time on the reactivity of calcined limestone material were investigated. In this study, the reactivity was measured using a pH stat apparatus and also confirming the result by performing citric acid reactivity test. The reactivity was calculated using the shrinking core model. Based on the experiments, a mathematical model is developed to correlate the effect of time and temperature to the reactivity of absorbent. The calcination process variables were temperature (700 -1000°C) and time (1-6 hrs). It was found that reactivity increases with an increase in time and temperature. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=reactivity" title="reactivity">reactivity</a>, <a href="https://publications.waset.org/abstracts/search?q=citric%20acid" title=" citric acid"> citric acid</a>, <a href="https://publications.waset.org/abstracts/search?q=calcination" title=" calcination"> calcination</a>, <a href="https://publications.waset.org/abstracts/search?q=time" title=" time"> time</a> </p> <a href="https://publications.waset.org/abstracts/57923/reactivity-study-on-south-african-calcium-based-material-using-a-ph-stat-and-citric-acid-a-statistical-approach" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/57923.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">220</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">26</span> The Retinoprotective Effects and Mechanisms of Fungal Ingredient 3,4-Dihydroxybenzalacetone through Inhibition of Retinal Müller and Microglial Activation</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Yu-Wen%20Cheng">Yu-Wen Cheng</a>, <a href="https://publications.waset.org/abstracts/search?q=Jau-Der%20Ho"> Jau-Der Ho</a>, <a href="https://publications.waset.org/abstracts/search?q=Liang-Huan%20Wu"> Liang-Huan Wu</a>, <a href="https://publications.waset.org/abstracts/search?q=Fan-Li%20Lin"> Fan-Li Lin</a>, <a href="https://publications.waset.org/abstracts/search?q=Li-Huei%20Chen"> Li-Huei Chen</a>, <a href="https://publications.waset.org/abstracts/search?q=Hung-Ming%20Chang"> Hung-Ming Chang</a>, <a href="https://publications.waset.org/abstracts/search?q=Yueh-Hsiung%20Kuo"> Yueh-Hsiung Kuo</a>, <a href="https://publications.waset.org/abstracts/search?q=George%20Hsiao"> George Hsiao</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Retina glial activation and neuroinflammation have been confirmed to cause devastating responses in retinodegenerative diseases. The expression and activation of matrix metalloproteinase (MMP)-9 and inducible nitric oxide synthase (iNOS) could be exerted as the crucial pathological factors in glaucoma- and blue light-induced retinal injuries. The present study aimed to investigate the retinoprotective effects and mechanisms of fungal ingredient 3,4-dihydroxybenzalacetone (DBL) isolated from Phellinus linteus in the retinal glial activation and retinodegenerative animal models. According to the cellular studies, DBL significantly and concentration-dependently abrogated MMP-9 activation and expression in TNFα-stimulated retinal Müller (rMC-1) cells. We found the inhibitory activities of DBL were strongly through the STAT- and ERK-dependent pathways. Furthermore, DBL dramatically attenuated MMP-9 activation in the stimulated Müller cells exposed to conditioned media from LPS-stimulated microglia BV-2 cells. On the other hand, DBL strongly suppressed LPS-induced production of NO and ROS and expression of iNOS in microglia BV-2 cells. Consistently, the phosphorylation of STAT was substantially blocked by DBL in LPS-stimulated microglia BV-2 cells. In the evaluation of retinoprotective functions, the high IOP-induced scotopic electroretinographic (ERG) deficit and blue light-induced abnormal pupillary light response (PLR) were assessed. The deficit scotopic ERG responses markedly recovered by DBL in a rat model of glaucoma-like ischemia/reperfusion (I/R)-injury. DBL also reduced the aqueous gelatinolytic activity and retinal MMP-9 expression in high IOP-injured conditions. Additionally, DBL could restore the abnormal PLR and reduce retinal MMP-9 activation. In summary, DBL could ameliorate retinal neuroinflammation and MMP-9 activation by predominantly inhibiting STAT3 activation in the retinal Müller cells and microglia, which exhibits therapeutic potential for glaucoma and other retinal degenerative diseases. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=glaucoma" title="glaucoma">glaucoma</a>, <a href="https://publications.waset.org/abstracts/search?q=blue%20light" title=" blue light"> blue light</a>, <a href="https://publications.waset.org/abstracts/search?q=DBL" title=" DBL"> DBL</a>, <a href="https://publications.waset.org/abstracts/search?q=retinal%20M%C3%BCller%20cell" title=" retinal Müller cell"> retinal Müller cell</a>, <a href="https://publications.waset.org/abstracts/search?q=MMP-9" title=" MMP-9"> MMP-9</a>, <a href="https://publications.waset.org/abstracts/search?q=STAT" title=" STAT"> STAT</a>, <a href="https://publications.waset.org/abstracts/search?q=Microglia" title=" Microglia"> Microglia</a>, <a href="https://publications.waset.org/abstracts/search?q=iNOS" title=" iNOS"> iNOS</a>, <a href="https://publications.waset.org/abstracts/search?q=ERG" title=" ERG"> ERG</a>, <a href="https://publications.waset.org/abstracts/search?q=PLR" title=" PLR"> PLR</a> </p> <a href="https://publications.waset.org/abstracts/136717/the-retinoprotective-effects-and-mechanisms-of-fungal-ingredient-34-dihydroxybenzalacetone-through-inhibition-of-retinal-muller-and-microglial-activation" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/136717.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">139</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">25</span> Blood Analysis of Diarrheal Calves Using Portable Blood Analyzer: Analysis of Calves by Age</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Kwangman%20Park">Kwangman Park</a>, <a href="https://publications.waset.org/abstracts/search?q=Jinhee%20Kang"> Jinhee Kang</a>, <a href="https://publications.waset.org/abstracts/search?q=Suhee%20Kim"> Suhee Kim</a>, <a href="https://publications.waset.org/abstracts/search?q=Dohyeon%20Yu"> Dohyeon Yu</a>, <a href="https://publications.waset.org/abstracts/search?q=Kyoungseong%20Choi"> Kyoungseong Choi</a>, <a href="https://publications.waset.org/abstracts/search?q=Jinho%20Park"> Jinho Park</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Statement of the Problem: Diarrhea is a major cause of death in young calves. This causes great economic damage to the livestock industry. These diarrhea cause dehydration, decrease blood flow, lower the pH and degrade enzyme function. In the past, serum screening was not possible in the field. However, now with the spread of portable serum testing devices, it is now possible to conduct tests directly on field. Thus, accurate serological changes can be identified and used in the field of large animals. Methodology and Theoretical Orientation: The test groups were calves from 1 to 44 days old. The status of the feces was divided into four grade to determine the severity of diarrhea (grade 0,1,2,3). Grade 0, 1 is considered to have no diarrhea. Grade 2, 3 is considered to diarrhea positive group. One or more viruses were detected in this group. Diarrhea negasitive group consisted of 57 calves (Asan=30, Samrye=27). Diarrhea positive group consisted of 34 calves (Kimje=27, Geochang=7). The feces of all calves were analyzed by PCR Test. Blood sample was measured using an automatic blood analyzer(i-STAT, Abbott inc. Illinois, US). Calves were divided into 3 groups according to age. Group 1 is 1 to 14 days old. Group 2 is 15 to 28 days old. Group 3 is more than 28 days old. Findings: Diarrhea caused an increase in HCT due to dehydration. The difference from normal was highest in 15 to 28 days old (p < 0.01). At all ages, bicarbonate decreased compared to normal, and therefore pH decreased. Similar to HCT, the largest difference was observed between 15 and 28 days (p < 0.01). The pCO₂ decreases to compensate for the decrease in pH. Conclusion and Significance: At all ages, HCT increases, and bicarbonate, pH, and pCO₂ decrease in diarrhea calves. The calf from 15 days to 28 days shows the most difference from normal. Over 28 days of age, weight gain and homeostasis ability increase, diarrhea is seen in the stool, there are fewer hematologic changes than groups below 28 days of age. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=calves" title="calves">calves</a>, <a href="https://publications.waset.org/abstracts/search?q=diarrhea" title=" diarrhea"> diarrhea</a>, <a href="https://publications.waset.org/abstracts/search?q=hematological%20changes" title=" hematological changes"> hematological changes</a>, <a href="https://publications.waset.org/abstracts/search?q=i-STAT" title=" i-STAT"> i-STAT</a> </p> <a href="https://publications.waset.org/abstracts/102455/blood-analysis-of-diarrheal-calves-using-portable-blood-analyzer-analysis-of-calves-by-age" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/102455.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">161</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">24</span> IL6/PI3K/mTOR/GFAP Molecular Pathway Role in COVID-19-Induced Neurodegenerative Autophagy, Impacts and Relatives</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mohammadjavad%20Sotoudeheian">Mohammadjavad Sotoudeheian</a> </p> <p class="card-text"><strong>Abstract:</strong></p> COVID-19, which began in December 2019, uses the angiotensin-converting enzyme 2 (ACE2) receptor to enter and spread through the cells. ACE2 mRNA is present in almost every organ, including nasopharynx, lung, as well as the brain. Ports of entry of SARS-CoV-2 into the central nervous system (CNS) may include arterial circulation, while viremia is remarkable. However, it is imperious to develop neurological symptoms evaluation CSF analysis in patients with COVID-19, but theoretically, ACE2 receptors are expressed in cerebellar cells and may be a target for SARS-CoV-2 infection in the brain. Recent evidence agrees that SARS-CoV-2 can impact the brain through direct and indirect injury. Two biomarkers for CNS injury, glial fibrillary acidic protein (GFAP) and neurofilament light chain (NFL) detected in the plasma of patients with COVID-19. NFL, an axonal protein expressed in neurons, is related to axonal neurodegeneration, and GFAP is over-expressed in CNS inflammation. GFAP cytoplasmic accumulation causes Schwan cells to misfunction, so affects myelin generation, reduces neuroskeletal support over NfLs during CNS inflammation, and leads to axonal degeneration. Interleukin-6 (IL-6), which extensively over-express due to interleukin storm during COVID-19 inflammation, regulates gene expression, as well as GFAP through STAT molecular pathway. IL-6 also impresses the phosphoinositide 3-kinase (PI3K)/STAT/smads pathway. The PI3K/ protein kinase B (Akt) pathway is the main modulator upstream of the mammalian target of rapamycin (mTOR), and alterations in this pathway are common in neurodegenerative diseases. Most neurodegenerative diseases show a disruption of autophagic function and display an abnormal increase in protein aggregation that promotes cellular death. Therefore, induction of autophagy has been recommended as a rational approach to help neurons clear abnormal protein aggregates and survive. The mTOR is a major regulator of the autophagic process and is regulated by cellular stressors. The mTORC1 pathway and mTORC2, as complementary and important elements in mTORC1 signaling, have become relevant in the regulation of the autophagic process and cellular survival through the extracellular signal-regulated kinase (ERK) pathway. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=mTORC1" title="mTORC1">mTORC1</a>, <a href="https://publications.waset.org/abstracts/search?q=COVID-19" title=" COVID-19"> COVID-19</a>, <a href="https://publications.waset.org/abstracts/search?q=PI3K" title=" PI3K"> PI3K</a>, <a href="https://publications.waset.org/abstracts/search?q=autophagy" title=" autophagy"> autophagy</a>, <a href="https://publications.waset.org/abstracts/search?q=neurodegeneration" title=" neurodegeneration"> neurodegeneration</a> </p> <a href="https://publications.waset.org/abstracts/162174/il6pi3kmtorgfap-molecular-pathway-role-in-covid-19-induced-neurodegenerative-autophagy-impacts-and-relatives" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/162174.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">86</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">23</span> Innate Immunity of Insects in Brief</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ehsan%20Soleymaninejadian">Ehsan Soleymaninejadian</a> </p> <p class="card-text"><strong>Abstract:</strong></p> As the field of immunology is growing day by day, and its chaotic system amazes more people, greed of research in this area is growing; however dealing with human or mammalian cells such as mice make the research expensive. Although there are some differences between higher animals with insects, importance of innate immunity during evolution made it untouched. So, for understanding the innate immunity insects can be good models. They are cheap; reproduction is fast and in the case genetics, less complicated. In this review, we tried to briefly tackle with important factors in insects’ innate immunity such as melanization, encapsulation, JAK-STAT, IMD, and Toll pathways. At the end, we explained how hormones and nerve system also can impact on immune system and make it more beautiful. In concluding remarks, the possibility of taking help from insect immune system to fight against diseases such as cancer has been considered. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=insects" title="insects">insects</a>, <a href="https://publications.waset.org/abstracts/search?q=innate%20immunity" title=" innate immunity"> innate immunity</a>, <a href="https://publications.waset.org/abstracts/search?q=melanization" title=" melanization"> melanization</a>, <a href="https://publications.waset.org/abstracts/search?q=intracellular%20pathways" title=" intracellular pathways"> intracellular pathways</a>, <a href="https://publications.waset.org/abstracts/search?q=hormones" title=" hormones"> hormones</a> </p> <a href="https://publications.waset.org/abstracts/74600/innate-immunity-of-insects-in-brief" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/74600.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">226</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">22</span> Impact of pH Control on Peptide Profile and Antigenicity of Whey Hydrolysates</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Natalia%20Caldeira%20De%20Carvalho">Natalia Caldeira De Carvalho</a>, <a href="https://publications.waset.org/abstracts/search?q=Tassia%20Batista%20Pessato"> Tassia Batista Pessato</a>, <a href="https://publications.waset.org/abstracts/search?q=Luis%20Gustavo%20R.%20Fernandes"> Luis Gustavo R. Fernandes</a>, <a href="https://publications.waset.org/abstracts/search?q=Ricardo%20L.%20Zollner"> Ricardo L. Zollner</a>, <a href="https://publications.waset.org/abstracts/search?q=Flavia%20Maria%20Netto"> Flavia Maria Netto</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Protein hydrolysates are ingredients of enteral diets and hypoallergenic formulas. Enzymatic hydrolysis is the most commonly used method for reducing the antigenicity of milk protein. The antigenicity and physicochemical characteristics of the protein hydrolysates depend on the reaction parameters. Among them, pH has been pointed out as of the major importance. Hydrolysis reaction in laboratory scale is commonly carried out under controlled pH (pH-stat). However, from the industrial point of view, controlling pH during hydrolysis reaction may be infeasible. This study evaluated the impact of pH control on the physicochemical properties and antigenicity of the hydrolysates of whey proteins with Alcalase. Whey protein isolate (WPI) solutions containing 3 and 7 % protein (w/v) were hydrolyzed with Alcalase 50 and 100 U g-1 protein at 60°C for 180 min. The reactions were carried out under controlled and uncontrolled pH conditions. Hydrolyses performed under controlled pH (pH-stat) were initially adjusted and maintained at pH 8.5. Hydrolyses carried out without pH control were initially adjusted to pH 8.5. Degree of hydrolysis (DH) was determined by OPA method, peptides profile was evaluated by HPLC-RP, and molecular mass distribution by SDS-PAGE/Tricine. The residual α-lactalbumin (α-La) and β-lactoglobulin (β-Lg) concentrations were determined using commercial ELISA kits. The specific IgE and IgG binding capacity of hydrolysates was evaluated by ELISA technique, using polyclonal antibodies obtained by immunization of female BALB/c mice with α-La, β-Lg and BSA. In hydrolysis under uncontrolled pH, the pH dropped from 8.5 to 7.0 during the first 15 min, remaining constant throughout the process. No significant difference was observed between the DH of the hydrolysates obtained under controlled and uncontrolled pH conditions. Although all hydrolysates showed hydrophilic character and low molecular mass peptides, hydrolysates obtained with and without pH control exhibited different chromatographic profiles. Hydrolysis under uncontrolled pH released, predominantly, peptides between 3.5 and 6.5 kDa, while hydrolysis under controlled pH released peptides smaller than 3.5 kDa. Hydrolysis with Alcalase under all conditions studied decreased by 99.9% the α-La and β-Lg concentrations in the hydrolysates detected by commercial kits. In general, β-Lg concentrations detected in the hydrolysates obtained under uncontrolled pH were significantly higher (p<0.05) than those detected in hydrolysates produced with pH control. The anti-α-La and anti-β-Lg IgE and IgG responses to all hydrolysates decreased significantly compared to WPI. Levels of specific IgE and IgG to the hydrolysates were below 25 and 12 ng ml-1, respectively. Despite the differences in peptide composition and α-La and β-Lg concentrations, no significant difference was found between IgE and IgG binding capacity of hydrolysates obtained with or without pH control. These results highlight the impact of pH on the hydrolysates characteristics and their concentrations of antigenic protein. Divergence between the antigen detection by commercial ELISA kits and specific IgE and IgG binding response was found in this study. This result shows that lower protein detection does not imply in lower protein antigenicity. Thus, the use of commercial kits for allergen contamination analysis should be cautious. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=allergy" title="allergy">allergy</a>, <a href="https://publications.waset.org/abstracts/search?q=enzymatic%20hydrolysis" title=" enzymatic hydrolysis"> enzymatic hydrolysis</a>, <a href="https://publications.waset.org/abstracts/search?q=milk%20protein" title=" milk protein"> milk protein</a>, <a href="https://publications.waset.org/abstracts/search?q=pH%20conditions" title=" pH conditions"> pH conditions</a>, <a href="https://publications.waset.org/abstracts/search?q=physicochemical%20characteristics" title=" physicochemical characteristics"> physicochemical characteristics</a> </p> <a href="https://publications.waset.org/abstracts/57211/impact-of-ph-control-on-peptide-profile-and-antigenicity-of-whey-hydrolysates" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/57211.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">303</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">21</span> Optimization of Leaching Properties of a Low-Grade Copper Ore Using Central Composite Design (CCD)</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Lawrence%20Koech">Lawrence Koech</a>, <a href="https://publications.waset.org/abstracts/search?q=Hilary%20Rutto"> Hilary Rutto</a>, <a href="https://publications.waset.org/abstracts/search?q=Olga%20Mothibedi"> Olga Mothibedi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Worldwide demand for copper has led to intensive search for methods of extraction and recovery of copper from different sources. The study investigates the leaching properties of a low-grade copper ore by optimizing the leaching variables using response surface methodology. The effects of key parameters, i.e., temperature, solid to liquid ratio, stirring speed and pH, on the leaching rate constant was investigated using a pH stat apparatus. A Central Composite Design (CCD) of experiments was used to develop a quadratic model which specifically correlates the leaching variables and the rate constant. The results indicated that the model is in good agreement with the experimental data with a correlation coefficient (R2) of 0.93. The temperature and solid to liquid ratio were found to have the most substantial influence on the leaching rate constant. The optimum operating conditions for copper leaching from the ore were identified as temperature at 65C, solid to liquid ratio at 1.625 and stirring speed of 325 rpm which yielded an average leaching efficiency of 93.16%. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=copper" title="copper">copper</a>, <a href="https://publications.waset.org/abstracts/search?q=leaching" title=" leaching"> leaching</a>, <a href="https://publications.waset.org/abstracts/search?q=CCD" title=" CCD"> CCD</a>, <a href="https://publications.waset.org/abstracts/search?q=rate%20constant" title=" rate constant"> rate constant</a> </p> <a href="https://publications.waset.org/abstracts/59859/optimization-of-leaching-properties-of-a-low-grade-copper-ore-using-central-composite-design-ccd" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/59859.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">242</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">20</span> Studying the Antiapoptotic Activity of Β Cells from Cord Blood Based Mesenchymal Stem Cells as an Approach to Treat Diabetes Mellitus</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Parcha%20Sreenivasa%20Rao">Parcha Sreenivasa Rao</a>, <a href="https://publications.waset.org/abstracts/search?q=P.%20Lakshmi"> P. Lakshmi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Diabetes Mellitus is metabolic disorder, characterized by high glucose levels in the blood due to one of the reason i.e., the death of β cells. The lack of β cells leads to the reduced insulin levels. The β cell death generally occurs due to apoptosis induced by the several cytokines. IL-1β, IFN- ϒ and TNF –α cytokines that are generally cause apoptosis to the β cell. The nutrient based apoptosis is generally seen with high glucose and free fatty acids. It is also noted that the β cell death triggered by Fas ligand and its receptor Fas at the surface of the activated CD8+ T- lymphocytes. Reports also reveal that the β cell apoptosis is under control of the transcription factors NF-kB and STAT- 1. The arresting or opposing of the β cell apoptosis can be overcome by the different growth factors like GLP-1, growth hormone, prolactin, VEGF, Dipeptidyl peptidase-4, Vildagliptin, suberoylanilidehydroxamic acid, trichistatin-A, XIAP, Bcl-2, FGF-21. Present investigation explains antiapoptotic property of the β cells derived from the mesenchymal stem cells of umbilical cord. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=stem%20cells" title="stem cells">stem cells</a>, <a href="https://publications.waset.org/abstracts/search?q=umblical%20cord" title=" umblical cord"> umblical cord</a>, <a href="https://publications.waset.org/abstracts/search?q=diabetes" title=" diabetes"> diabetes</a>, <a href="https://publications.waset.org/abstracts/search?q=apoptosis" title=" apoptosis"> apoptosis</a> </p> <a href="https://publications.waset.org/abstracts/39952/studying-the-antiapoptotic-activity-of-b-cells-from-cord-blood-based-mesenchymal-stem-cells-as-an-approach-to-treat-diabetes-mellitus" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/39952.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">381</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">19</span> Effect of Oat-Protein Peptide in Cognitive Impairment Mice via Mediating Gut-Brain Axis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Hamad%20Rafique">Hamad Rafique</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The bioactive peptide RDFPITWPW (RW-9) identified from oat protein has been reported to be positive in memory deficits. However, no clarity on the mechanisms responsible for the neuroprotective effects of RW-9 peptide against AD-like symptoms. Herein, it found that RW-9 intervention showed various improving effects in cognitive-behavioral tests and alleviated oxidative stress and inflammation in the scopolamine-induced mice model. The hippocampus proteomics analysis revealed the upregulation of memory-related proteins, including Grin3a, Ppp2r1b, Stat6, Pik3cd, Slc5a7, Chrm2, mainly involved in cAMP signaling, PI3K-Akt signaling, and JAK-STAT signaling pathways. The administration of RW-9 significantly upregulated the neurotransmitters, including 5-HT, DA, and Arg, in mice brains. Moreover, it regulated the serum metabolic profile and increased the expression levels of ABC transporters, biosynthesis of amino acids, and Amino acyl-tRNA biosynthesis, among others. The 16s-rRNA results illustrated that the RW-9 restored the abundance of Muribaculaceae, Lachnospiraceae, Lactobacillus, Clostridia and Bactericides. Taken together, our results suggest that the RW-9 may prevent the AD-like symptoms via modulation of the gut-serum-brain axis. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=oat%20protein" title="oat protein">oat protein</a>, <a href="https://publications.waset.org/abstracts/search?q=active%20peptide" title=" active peptide"> active peptide</a>, <a href="https://publications.waset.org/abstracts/search?q=neuroprotective" title=" neuroprotective"> neuroprotective</a>, <a href="https://publications.waset.org/abstracts/search?q=gut-brain%20axis" title=" gut-brain axis"> gut-brain axis</a> </p> <a href="https://publications.waset.org/abstracts/189320/effect-of-oat-protein-peptide-in-cognitive-impairment-mice-via-mediating-gut-brain-axis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/189320.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">27</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">18</span> Unique Interprofessional Mental Health Education Model: A Pre/Post Survey</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Michele%20L.%20Tilstra">Michele L. Tilstra</a>, <a href="https://publications.waset.org/abstracts/search?q=Tiffany%20J.%20Peets"> Tiffany J. Peets</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Interprofessional collaboration in behavioral healthcare education is increasingly recognized for its value in training students to address diverse client needs. While interprofessional education (IPE) is well-documented in occupational therapy education to address physical health, limited research exists on collaboration with counselors to address mental health concerns and the psychosocial needs of individuals receiving care. Counseling education literature primarily examines the collaboration of counseling students with psychiatrists, psychologists, social workers, and marriage and family therapists. This pretest/posttest survey research study explored changes in attitudes toward interprofessional teams among 56 Master of Occupational Therapy (MOT) (n = 42) and Counseling and Human Development (CHD) (n = 14) students participating in the Counselors and Occupational Therapists Professionally Engaged in the Community (COPE) program. The COPE program was designed to strengthen the behavioral health workforce in high-need and high-demand areas. Students accepted into the COPE program were divided into small MOT/CHD groups to complete multiple interprofessional multicultural learning modules using videos, case studies, and online discussion board posts. The online modules encouraged reflection on various behavioral healthcare roles, benefits of team-based care, cultural humility, current mental health challenges, personal biases, power imbalances, and advocacy for underserved populations. Using the Student Perceptions of Interprofessional Clinical Education- Revision 2 (SPICE-R2) scale, students completed pretest and posttest surveys using a 5-point Likert scale (Strongly Agree = 5 to Strongly Disagree = 1) to evaluate their attitudes toward interprofessional teamwork and collaboration. The SPICE-R2 measured three different factors: interprofessional teamwork and team-based practice (Team), roles/responsibilities for collaborative practice (Roles), and patient outcomes from collaborative practice (Outcomes). The mean total scores for all students improved from 4.25 (pretest) to 4.43 (posttest), Team from 4.66 to 4.58, Roles from 3.88 to 4.30, and Outcomes from 4.08 to 4.36. A paired t-test analysis for the total mean scores resulted in a t-statistic of 2.54, which exceeded both one-tail and two-tail critical values, indicating statistical significance (p = .001). When the factors of the SPICE-R2 were analyzed separately, only the Roles (t Stat=4.08, p =.0001) and Outcomes (t Stat=3.13, p = .002) were statistically significant. The item ‘I understand the roles of other health professionals’ showed the most improvement from a mean score for all students of 3.76 (pretest) to 4.46 (posttest). The significant improvement in students' attitudes toward interprofessional teams suggests that the unique integration of OT and CHD students in the COPE program effectively develops a better understanding of the collaborative roles necessary for holistic client care. These results support the importance of IPE through structured, engaging interprofessional experiences. These experiences are essential for enhancing students' readiness for collaborative practice and align with accreditation standards requiring interprofessional education in OT and CHD programs to prepare practitioners for team-based care. The findings contribute to the growing body of evidence supporting the integration of IPE in behavioral healthcare curricula to improve holistic client care and encourage students to engage in collaborative practice across healthcare settings. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=behavioral%20healthcare" title="behavioral healthcare">behavioral healthcare</a>, <a href="https://publications.waset.org/abstracts/search?q=counseling%20education" title=" counseling education"> counseling education</a>, <a href="https://publications.waset.org/abstracts/search?q=interprofessional%20education" title=" interprofessional education"> interprofessional education</a>, <a href="https://publications.waset.org/abstracts/search?q=mental%20health%20education" title=" mental health education"> mental health education</a>, <a href="https://publications.waset.org/abstracts/search?q=occupational%20therapy%20education" title=" occupational therapy education"> occupational therapy education</a> </p> <a href="https://publications.waset.org/abstracts/186548/unique-interprofessional-mental-health-education-model-a-prepost-survey" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/186548.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">39</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">17</span> Optimization of Monascus Orange Pigments Production Using pH-Controlled Fed-Batch Fermentation</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Young%20Min%20Kim">Young Min Kim</a>, <a href="https://publications.waset.org/abstracts/search?q=Deokyeong%20Choe"> Deokyeong Choe</a>, <a href="https://publications.waset.org/abstracts/search?q=Chul%20Soo%20Shin"> Chul Soo Shin</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Monascus pigments, commonly used as a natural colorant in Asia, have many biological activities, such as cholesterol level control, anti-obesity, anti-cancer, and anti-oxidant, that have recently been elucidated. Especially, amino acid derivatives of Monascus pigments are receiving much attention because they have higher biological activities than original Monascus pigments. Previously, there have been two ways to produce amino acid derivatives: one-step production and two-step production. However, the one-step production has low purity, and the two-step production—precursor(orange pigments) fermentation and derivatives synthesis—has low productivity and growth rate during its precursor fermentation step. In this study, it was verified that pH is a key factor that affects the stability of orange pigments and the growth rate of Monascus. With an optimal pH profile obtained by pH-stat fermentation, we designed a process of precursor(orange pigments) fermentation that is a pH-controlled fed-batch fermentation. The final concentration of orange pigments in this process increased to 5.5g/L which is about 30% higher than the concentration produced from the previously used precursor fermentation step. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cultivation%20process" title="cultivation process">cultivation process</a>, <a href="https://publications.waset.org/abstracts/search?q=fed-batch%20fermentation" title=" fed-batch fermentation"> fed-batch fermentation</a>, <a href="https://publications.waset.org/abstracts/search?q=monascus%20pigments" title=" monascus pigments"> monascus pigments</a>, <a href="https://publications.waset.org/abstracts/search?q=pH%20stability" title=" pH stability"> pH stability</a> </p> <a href="https://publications.waset.org/abstracts/55435/optimization-of-monascus-orange-pigments-production-using-ph-controlled-fed-batch-fermentation" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/55435.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">299</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">16</span> Network Based Molecular Profiling of Intracranial Ependymoma over Spinal Ependymoma</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Hyeon%20Su%20Kim">Hyeon Su Kim</a>, <a href="https://publications.waset.org/abstracts/search?q=Sungjin%20Park"> Sungjin Park</a>, <a href="https://publications.waset.org/abstracts/search?q=Hae%20Ryung%20Chang"> Hae Ryung Chang</a>, <a href="https://publications.waset.org/abstracts/search?q=Hae%20Rim%20Jung"> Hae Rim Jung</a>, <a href="https://publications.waset.org/abstracts/search?q=Young%20Zoo%20Ahn"> Young Zoo Ahn</a>, <a href="https://publications.waset.org/abstracts/search?q=Yon%20Hui%20Kim"> Yon Hui Kim</a>, <a href="https://publications.waset.org/abstracts/search?q=Seungyoon%20Nam"> Seungyoon Nam</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Ependymoma, one of the most common parenchymal spinal cord tumor, represents 3-6% of all CNS tumor. Especially intracranial ependymomas, which are more frequent in childhood, have a more poor prognosis and more malignant than spinal ependymomas. Although there are growing needs to understand pathogenesis, detailed molecular understanding of pathogenesis remains to be explored. A cancer cell is composed of complex signaling pathway networks, and identifying interaction between genes and/or proteins are crucial for understanding these pathways. Therefore, we explored each ependymoma in terms of differential expressed genes and signaling networks. We used Microsoft Excel™ to manipulate microarray data gathered from NCBI’s GEO Database. To analyze and visualize signaling network, we used web-based PATHOME algorithm and Cytoscape. We show HOX family and NEFL are down-regulated but SCL family is up-regulated in cerebrum and posterior fossa cancers over a spinal cancer, and JAK/STAT signaling pathway and Chemokine signaling pathway are significantly different in the both intracranial ependymoma comparing to spinal ependymoma. We are considering there may be an age-dependent mechanism under different histological pathogenesis. We annotated mutation data of each gene subsequently in order to find potential target genes. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=systems%20biology" title="systems biology">systems biology</a>, <a href="https://publications.waset.org/abstracts/search?q=ependymoma" title=" ependymoma"> ependymoma</a>, <a href="https://publications.waset.org/abstracts/search?q=deg" title=" deg"> deg</a>, <a href="https://publications.waset.org/abstracts/search?q=network%20analysis" title=" network analysis"> network analysis</a> </p> <a href="https://publications.waset.org/abstracts/50168/network-based-molecular-profiling-of-intracranial-ependymoma-over-spinal-ependymoma" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/50168.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">298</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">15</span> Qualitative Data Analysis for Health Care Services</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Taner%20Ersoz">Taner Ersoz</a>, <a href="https://publications.waset.org/abstracts/search?q=Filiz%20Ersoz"> Filiz Ersoz</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This study was designed enable application of multivariate technique in the interpretation of categorical data for measuring health care services satisfaction in Turkey. The data was collected from a total of 17726 respondents. The establishment of the sample group and collection of the data were carried out by a joint team from The Ministry of Health and Turkish Statistical Institute (Turk Stat) of Turkey. The multiple correspondence analysis (MCA) was used on the data of 2882 respondents who answered the questionnaire in full. The multiple correspondence analysis indicated that, in the evaluation of health services females, public employees, younger and more highly educated individuals were more concerned and complainant than males, private sector employees, older and less educated individuals. Overall 53 % of the respondents were pleased with the improvements in health care services in the past three years. This study demonstrates the public consciousness in health services and health care satisfaction in Turkey. It was found that most the respondents were pleased with the improvements in health care services over the past three years. Awareness of health service quality increases with education levels. Older individuals and males would appear to have lower expectancies in health services. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=multiple%20correspondence%20analysis" title="multiple correspondence analysis">multiple correspondence analysis</a>, <a href="https://publications.waset.org/abstracts/search?q=multivariate%20categorical%20data" title=" multivariate categorical data"> multivariate categorical data</a>, <a href="https://publications.waset.org/abstracts/search?q=health%20care%20services" title=" health care services"> health care services</a>, <a href="https://publications.waset.org/abstracts/search?q=health%20satisfaction%20survey" title=" health satisfaction survey"> health satisfaction survey</a> </p> <a href="https://publications.waset.org/abstracts/75502/qualitative-data-analysis-for-health-care-services" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/75502.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">242</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">14</span> Formulation and Technology of the Composition of Essential Oils as a Feed Additive in Poultry with Antibacterial Action</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=S.%20Barbaqadze">S. Barbaqadze</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Goderdzishvili"> M. Goderdzishvili</a>, <a href="https://publications.waset.org/abstracts/search?q=E.%20Mosidze"> E. Mosidze</a>, <a href="https://publications.waset.org/abstracts/search?q=L.%20Lomtadze"> L. Lomtadze</a>, <a href="https://publications.waset.org/abstracts/search?q=V.%20Mshvildadze"> V. Mshvildadze</a>, <a href="https://publications.waset.org/abstracts/search?q=L.%20Bakuridze"> L. Bakuridze</a>, <a href="https://publications.waset.org/abstracts/search?q=D.%20Berashvili"> D. Berashvili</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20Bakuridze"> A. Bakuridze</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This paper focuses on the formulation of phytobiotic designated for further implantation in poultry farming. Composition was meant to be water-soluble powder containing antibacterial essential oils. The development process involved Thyme, Monarda and Clary sage essential oils. The antimicrobial activity of essential oils composite was meant to be tested against gram-negative and gram-positive bacterial strains. The results are processed using the statistical program Sigma STAT. To make essential oils composition water soluble surfactants were added to them. At the first stage of the study, nine options for the optimal composition of essential oils and surfactants were developed. The effect of the amount of surfactants on the essential oils composition solubility in water has been investigated. On the basis of biopharmaceutical studies, the formulation of phytobiotic has been determined: Thyme, monarda and clary sage essential oils 2:1:1 - 100 parts; Licorice extract 5.25 parts and inhalation lactose 300 parts. A technology for the preparation of phytobiotic has been developed and a technological scheme for the preparation of phytobiotic has been made up. The research was performed within the framework of the grant project CARYS-19-363 funded be the Shota Rustaveli National Science Foundation of Georgia. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=clary" title="clary">clary</a>, <a href="https://publications.waset.org/abstracts/search?q=essential%20oils" title=" essential oils"> essential oils</a>, <a href="https://publications.waset.org/abstracts/search?q=monarda" title=" monarda"> monarda</a>, <a href="https://publications.waset.org/abstracts/search?q=phytobiotics" title=" phytobiotics"> phytobiotics</a>, <a href="https://publications.waset.org/abstracts/search?q=poultry" title=" poultry"> poultry</a>, <a href="https://publications.waset.org/abstracts/search?q=thyme" title=" thyme"> thyme</a> </p> <a href="https://publications.waset.org/abstracts/133039/formulation-and-technology-of-the-composition-of-essential-oils-as-a-feed-additive-in-poultry-with-antibacterial-action" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/133039.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">160</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">13</span> Formulation, Evaluation and Statistical Optimization of Transdermal Niosomal Gel of Atenolol</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Lakshmi%20Sirisha%20Kotikalapudi">Lakshmi Sirisha Kotikalapudi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Atenolol, the widely used antihypertensive drug is ionisable and degrades in the acidic environment of the GIT lessening the bioavailability. Transdermal route may be selected as an alternative to enhance the bioavailability. Half-life of the drug is 6-7 hours suggesting the requirement of prolonged release of the drug. The present work of transdermal niosomal gel aims to extend release of the drug and increase the bioavailability. Ethanol injection method was used for the preparation of niosomes using span-60 and cholesterol at different molar ratios following central composite design. The prepared niosomes were characterized for size, zeta-potential, entrapment efficiency, drug content and in-vitro drug release. Optimized formulation was selected by statistically analyzing the results obtained using the software Stat-Ease Design Expert. The optimized formulation also showed high drug retention inside the vesicles over a period of three months at a temperature of 4 °C indicating stability. Niosomes separated as a pellet were dried and incorporated into the hydrogel prepared using chitosan a natural polymer as a gelling agent. The effect of various chemical permeation enhancers was also studied over the gel formulations. The prepared formulations were characterized for viscosity, pH, drug release using Franz diffusion cells, and skin irritation test as well as in-vivo pharmacological activities. Atenolol niosomal gel preparations showed the prolonged release of the drug and pronounced antihypertensive activity indicating the suitability of niosomal gel for topical and systemic delivery of atenolol. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=atenolol" title="atenolol">atenolol</a>, <a href="https://publications.waset.org/abstracts/search?q=chitosan" title=" chitosan"> chitosan</a>, <a href="https://publications.waset.org/abstracts/search?q=niosomes" title=" niosomes"> niosomes</a>, <a href="https://publications.waset.org/abstracts/search?q=transdermal" title=" transdermal"> transdermal</a> </p> <a href="https://publications.waset.org/abstracts/59549/formulation-evaluation-and-statistical-optimization-of-transdermal-niosomal-gel-of-atenolol" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/59549.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">295</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">12</span> Corrosion Behaviour of Al-Mg-Si Alloy Matrix Hybrid Composite Reinforced with Cassava Peel Ash and Silicon Carbide</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=B.%20Oji">B. Oji</a>, <a href="https://publications.waset.org/abstracts/search?q=O.%20Olaniran"> O. Olaniran</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The prospect of improving the corrosion property of Al 6063 alloy based hybrid composites reinforced with cassava peel ash (CPA) and silicon carbide (SiC) is the target of this research. It seeks to determine the viability of using locally sourced material (CPA) as a complimentary reinforcement for SiC to produce low cost high performance aluminum matrix composite. The CPA was mixed with the SiC in the ratios 0:1, 1:3, 1:1, 3:1 and 1:0 for 8 wt % reinforcement in the produced composites by double stir-casting method. The microstructures of the composites were studied before and after corrosion using the scanning electron microscopy which reveals the matrix (dark region) and eutectic phase (lamellar region). The corrosion rate was studied in accordance with ASTM G59-97 (2014) using an AutoLab potentiostat (Versa STAT 400) with versaSTUDIO electrochemical software which analyses the results obtained. The result showed that Al 6063 alloy exhibited good corrosion resistance in 0.3M H₂SO₄ and 3.5 wt. % NaCl solutions with sample C containing the 25% wt CPA showing the highest resistance to corrosion with corrosion rate of 0.0046 mmpy as compared to the control sample which has a value of 13.233 mmpy. Sample B, D, E, and F also showed a corrosion rate of 3.9502, 2.6903, 2.1223, and 5.7344 mmpy which indicated a better corrosion rate than the control in the acidic environment. The corrosion rate in the saline medium shows that sample E with 75% wt CPA has the lowest corrosion rate of 0.0422 mmpy as compared to the control sample with 0.0873 mmpy corrosion rate. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Al-Mg-Si%20alloy" title="Al-Mg-Si alloy">Al-Mg-Si alloy</a>, <a href="https://publications.waset.org/abstracts/search?q=AutoLab%20potentiostat" title=" AutoLab potentiostat"> AutoLab potentiostat</a>, <a href="https://publications.waset.org/abstracts/search?q=Cassava%20Peel%20Ash" title=" Cassava Peel Ash"> Cassava Peel Ash</a>, <a href="https://publications.waset.org/abstracts/search?q=CPA" title=" CPA"> CPA</a>, <a href="https://publications.waset.org/abstracts/search?q=hybrid%20composite" title=" hybrid composite"> hybrid composite</a>, <a href="https://publications.waset.org/abstracts/search?q=stir-cast%20method" title=" stir-cast method"> stir-cast method</a> </p> <a href="https://publications.waset.org/abstracts/108972/corrosion-behaviour-of-al-mg-si-alloy-matrix-hybrid-composite-reinforced-with-cassava-peel-ash-and-silicon-carbide" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/108972.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">127</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">11</span> Thrombocytopenia and Prolonged Prothrombin Time in Neonatal Septicemia</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Shittu%20Bashirat">Shittu Bashirat</a>, <a href="https://publications.waset.org/abstracts/search?q=Shittu%20Mujeeb"> Shittu Mujeeb</a>, <a href="https://publications.waset.org/abstracts/search?q=Oluremi%20Adeolu"> Oluremi Adeolu</a>, <a href="https://publications.waset.org/abstracts/search?q=Orisadare%20Olayiwola"> Orisadare Olayiwola</a>, <a href="https://publications.waset.org/abstracts/search?q=Jikeme%20Osameke"> Jikeme Osameke</a>, <a href="https://publications.waset.org/abstracts/search?q=Bello%20Lateef"> Bello Lateef</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Septicemia in neonates refers to generalized bacterial infection documented by positive blood culture in the first 28 days of life and is one of the leading causes of neonatal mortality in sub-Sahara Africa. Thrombocytopenia in newborns is a result of increased platelet consumption; sepsis was found to be the most common risk factor. The objective of the study was to determine if there are organism-specific platelet responses among the 2 groups of bacterial agents: Gram-positive and Gram-negative bacteria, and also to examine the association of platelet count and prothrombin time with neonatal septicemia. 232 blood samples were collected for this study. The blood culture was performed using Bactec 9050, an instrumented blood culture system. The platelet count and prothrombin time were performed using Abacus Junior 5 hematology analyzer and i-STAT 1 analyzer respectively. Of the 231 neonates hospitalized with clinical sepsis, blood culture reports were positive in 51 cases (21.4%). Klebsiella spp. (35.3%) and Staphylococcus aureus (27.5%) were the most common Gram-negative and Gram-positive isolates respectively. Thrombocytopenia was observed in 30 (58.8%) of the neonates with septicemia. Of the 9 (17.6%) patients with severe thrombocytopenia, seven (77.8%) had Klebsiella spp. septicemia. Out of the 21(63.6%) of thrombocytopenia produced by Gram-negative isolate, 17 (80.9) had increased prothrombin time. In conclusion, Gram-negative organisms showed the highest cases of severe thrombocytopenia and prolonged PT. This study has helped to establish a disturbance in hemostatic systems in neonates with septicemia. Further studies, however, may be required to assess other hemostasis parameters in order to understand their interaction with the infectious organisms in neonates. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=neonates" title="neonates">neonates</a>, <a href="https://publications.waset.org/abstracts/search?q=septicemia" title=" septicemia"> septicemia</a>, <a href="https://publications.waset.org/abstracts/search?q=thrombocytopenia" title=" thrombocytopenia"> thrombocytopenia</a>, <a href="https://publications.waset.org/abstracts/search?q=prolonged%20prothrombin%20time" title=" prolonged prothrombin time"> prolonged prothrombin time</a>, <a href="https://publications.waset.org/abstracts/search?q=platelet%20count" title=" platelet count"> platelet count</a> </p> <a href="https://publications.waset.org/abstracts/12120/thrombocytopenia-and-prolonged-prothrombin-time-in-neonatal-septicemia" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/12120.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">406</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">10</span> Comparison of the Results of a Parkinson’s Holter Monitor with Patient Diaries, in Real Conditions of Use: A Sub-Analysis of the MoMoPa-EC Clinical Trial</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Alejandro%20Rodr%C3%ADguez-Molinero">Alejandro Rodríguez-Molinero</a>, <a href="https://publications.waset.org/abstracts/search?q=Carlos%20P%C3%A9rez-L%C3%B3pez"> Carlos Pérez-López</a>, <a href="https://publications.waset.org/abstracts/search?q=Jorge%20Hern%C3%A1ndez-Vara"> Jorge Hernández-Vara</a>, <a href="https://publications.waset.org/abstracts/search?q=%C3%80ngels%20Bayes-Rusi%C3%B1ol"> Àngels Bayes-Rusiñol</a>, <a href="https://publications.waset.org/abstracts/search?q=Juan%20Carlos%20Mart%C3%ADnez-Castrillo"> Juan Carlos Martínez-Castrillo</a>, <a href="https://publications.waset.org/abstracts/search?q=David%20A.%20P%C3%A9rez-Mart%C3%ADnez"> David A. Pérez-Martínez</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: Monitoring motor symptoms in Parkinson's patients is often a complex and time-consuming task for clinicians, as Hauser's diaries are often poorly completed by patients. Recently, new automatic devices (Parkinson's holter: STAT-ON®) have been developed capable of monitoring patients' motor fluctuations. The MoMoPa-EC clinical trial (NCT04176302) investigates which of the two methods produces better clinical results. In this sub-analysis, the concordance between both methods is analyzed. Methods: In the MoMoPa-EC clinical trial, 164 patients with moderate-severe Parkinson's disease and at least two hours a day of Off will be included. At the time of patient recruitment, all of them completed a seven-day motor fluctuation diary at home (Hauser’s diary) while wearing the Parkinson's holter. In this sub-analysis, 71 patients with complete data for the purpose of this comparison were included. The intraclass correlation coefficient was calculated between the patient diary entries and the Parkinson's holter data in terms of time On, Off, and time with dyskinesias. Results: The intra-class correlation coefficient of both methods was 0.57 (95% CI: 0.3-0.74) for daily time in Off (%), 0.48 (95% CI: 0.14-0.68) for daily time in On (%), and 0.37 (95% CI %: -0.04-0.62) for daily time with dyskinesias (%). Conclusions: Both methods have a moderate agreement with each other. We will have to wait for the results of the MoMoPa-EC project to estimate which of them has the greatest clinical benefits. Acknowledgment: This work is supported by AbbVie S.L.U, the Instituto de Salud Carlos III [DTS17/00195], and the European Fund for Regional Development, 'A way to make Europe'. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Parkinson" title="Parkinson">Parkinson</a>, <a href="https://publications.waset.org/abstracts/search?q=sensor" title=" sensor"> sensor</a>, <a href="https://publications.waset.org/abstracts/search?q=motor%20fluctuations" title=" motor fluctuations"> motor fluctuations</a>, <a href="https://publications.waset.org/abstracts/search?q=dyskinesia" title=" dyskinesia"> dyskinesia</a> </p> <a href="https://publications.waset.org/abstracts/142014/comparison-of-the-results-of-a-parkinsons-holter-monitor-with-patient-diaries-in-real-conditions-of-use-a-sub-analysis-of-the-momopa-ec-clinical-trial" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/142014.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">232</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">9</span> Acanthopanax koreanum and Major Ingredient, Impressic Acid, Possess Matrix Metalloproteinase-13 Down-Regulating Capacity and Protect Cartilage Destruction</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Hyun%20Lim">Hyun Lim</a>, <a href="https://publications.waset.org/abstracts/search?q=Dong%20Sook%20Min"> Dong Sook Min</a>, <a href="https://publications.waset.org/abstracts/search?q=Han%20Eul%20Yun"> Han Eul Yun</a>, <a href="https://publications.waset.org/abstracts/search?q=Kil%20Tae%20Kim"> Kil Tae Kim</a>, <a href="https://publications.waset.org/abstracts/search?q=Ya%20Nan%20Sun"> Ya Nan Sun</a>, <a href="https://publications.waset.org/abstracts/search?q=Young%20Ho%20Kim"> Young Ho Kim</a>, <a href="https://publications.waset.org/abstracts/search?q=Hyun%20Pyo%20Kim"> Hyun Pyo Kim</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Matrix metalloproteinase (MMP)-13 has an important role for degrading cartilage materials under inflammatory conditions such as arthritis. Since the 70% ethanol extract of Acanthopanax koreanum inhibited MMP-13 expression in IL-1β-treated human chondrocyte cell line, SW1353, two major constituents including acanthoic acid and impressic acid were initially isolated from the same plant materials and their MMP-13 down-regulating capacity was examined. In IL-1β-treated SW1353 cells, acanthoic acid and impressic acid significantly and concentration-dependently inhibited MMP-13 expression at 10 – 100 μM and 0.5 – 10 μM, respectively. The potent one, impressic acid, was found to inhibit MMP-13 expression by blocking the phosphorylation of signal transducer and activator of transcription-1/-2 (STAT-1/-2) and activation of c-Jun and c-Fos among cellular signaling pathway involved, but did not affect the activation of mitogen-activated protein kinases (MAPKs) and nuclear transcription factor-κB (NF-κB). Further, impressic acid was also found to inhibit the expression of MMP-13 mRNA (47.7% inhibition at 10 μM), the glycosaminoglycan release (42.2% reduction at 10 μM) and proteoglycan loss in IL-1-treated rabbit cartilage explants culture. For a further study, 21 impressic acid derivatives were isolated from the same plant materials and their suppressive activities against MMP-13 expression were examined. Among the derivatives, 3α-hydroxy-lup-20(29)-en-23-oxo,28-oic acid, (20R)-3α-hydroxy-29-dimethoxylupan-23,28-dioic acid, acankoreoside F and acantrifoside A clearly down-regulated MMP-13 expression, but impressic acid being most potent. All these results suggest that impressic acid, 3α-hydroxy-lup-20(29)-en-23-oxo,28-oic acid, (20R)-3α-hydroxy-29-dimethoxylupan-23,28-dioic acid, acankoreoside F, acantrifoside A and A. koreanum may have a potential for therapeutic agents to prevent cartilage degradation possibly by inhibiting matrix protein degradation. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=acanthoic%20acid" title="acanthoic acid">acanthoic acid</a>, <a href="https://publications.waset.org/abstracts/search?q=Acanthopanax%20koreanum" title=" Acanthopanax koreanum"> Acanthopanax koreanum</a>, <a href="https://publications.waset.org/abstracts/search?q=cartilage" title=" cartilage"> cartilage</a>, <a href="https://publications.waset.org/abstracts/search?q=impressic%20acid" title=" impressic acid"> impressic acid</a>, <a href="https://publications.waset.org/abstracts/search?q=matrix%20metalloproteinase" title=" matrix metalloproteinase"> matrix metalloproteinase</a> </p> <a href="https://publications.waset.org/abstracts/57571/acanthopanax-koreanum-and-major-ingredient-impressic-acid-possess-matrix-metalloproteinase-13-down-regulating-capacity-and-protect-cartilage-destruction" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/57571.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">361</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8</span> Deficiencies in Vitamin A and Iron Supply Potential of Selected Indigenous Complementary Foods of Infants in Uganda</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Richard%20Kajjura">Richard Kajjura</a>, <a href="https://publications.waset.org/abstracts/search?q=Joyce%20Kikafunda"> Joyce Kikafunda</a>, <a href="https://publications.waset.org/abstracts/search?q=Roger%20Whitehead"> Roger Whitehead</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Introduction: Indigenous complementary recipes for children (6-23 months) are bulky and inextricably linked. The potential contribution of indigenous complementary foods to infant’s vitamin A and iron needs is not well investigated in Uganda. Less is known whether children in Uganda are living with or without adequate supply of vitamin A and iron nutrients. In this study, vitamin A and iron contents were assessed in the complementary foods fed to infants aged 6-11 months in a Peri-urban setting in Kampala District in Central Uganda. Objective: Assessment of vitamin A and iron contents of indigenous complementary foods of children as fed and associated demographic factor. Method: In a cross sectional study design, one hundred and three (153) households with children aged 6-11 months were randomly selected to participate in the assessment. Complementary food samples were collected from the children’s mothers/caretakers at the time of feeding the child. The mothers’ socio-demographic characteristics of age, education, marital status, occupation and sex collected a semi-qualitative questionnaire. The Vitamin A and iron contents in the complementary foods were analyzed using a UV/VIS spectrophotometer for vitamin A and Atomic Absorption spectrophotometer for iron samples. The data was analyzed using Gene-stat software program. Results: The mean vitamin A content was 97.0± 72.5 µg while that of iron was 1.5 ± 0.4 mg per 100g of food sample as fed. The contribution of indigenous complementary foods found was 32% for vitamin A and 15% iron of the recommended dietary allowance. Age of children was found to be significantly associated Vitamin A and Iron supply potential. Conclusion: The contribution of indigenous complementary foods to infant’s vitamin A and iron needs was low. Complementary foods in Uganda are more likely to be deficient in vitamin A and iron content. Nutrient dense dietary supplementation should be intervened in to make possible for Ugandan children attain full growth potential. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=indigenous%20complementary%20food" title="indigenous complementary food">indigenous complementary food</a>, <a href="https://publications.waset.org/abstracts/search?q=infant" title=" infant"> infant</a>, <a href="https://publications.waset.org/abstracts/search?q=iron" title=" iron"> iron</a>, <a href="https://publications.waset.org/abstracts/search?q=vitamin%20A" title=" vitamin A "> vitamin A </a> </p> <a href="https://publications.waset.org/abstracts/33482/deficiencies-in-vitamin-a-and-iron-supply-potential-of-selected-indigenous-complementary-foods-of-infants-in-uganda" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/33482.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">478</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7</span> Screening of Factors Affecting the Enzymatic Hydrolysis of Empty Fruit Bunches in Aqueous Ionic Liquid and Locally Produced Cellulase System</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Md.%20Z.%20Alam">Md. Z. Alam</a>, <a href="https://publications.waset.org/abstracts/search?q=Amal%20A.%20Elgharbawy"> Amal A. Elgharbawy</a>, <a href="https://publications.waset.org/abstracts/search?q=Muhammad%20Moniruzzaman"> Muhammad Moniruzzaman</a>, <a href="https://publications.waset.org/abstracts/search?q=Nassereldeen%20A.%20Kabbashi"> Nassereldeen A. Kabbashi</a>, <a href="https://publications.waset.org/abstracts/search?q=Parveen%20Jamal"> Parveen Jamal</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The enzymatic hydrolysis of lignocellulosic biomass is one of the obstacles in the process of sugar production, due to the presence of lignin that protects the cellulose molecules against cellulases. Although the pretreatment of lignocellulose in ionic liquid (IL) system has been receiving a lot of interest; however, it requires IL removal with an anti-solvent in order to proceed with the enzymatic hydrolysis. At this point, introducing a compatible cellulase enzyme seems more efficient in this process. A cellulase enzyme that was produced by Trichoderma reesei on palm kernel cake (PKC) exhibited a promising stability in several ILs. The enzyme called PKC-Cel was tested for its optimum pH and temperature as well as its molecular weight. One among evaluated ILs, 1,3-diethylimidazolium dimethyl phosphate [DEMIM] DMP was applied in this study. Evaluation of six factors was executed in Stat-Ease Design Expert V.9, definitive screening design, which are IL/ buffer ratio, temperature, hydrolysis retention time, biomass loading, cellulase loading and empty fruit bunches (EFB) particle size. According to the obtained data, IL-enzyme system shows the highest sugar concentration at 70 °C, 27 hours, 10% IL-buffer, 35% biomass loading, 60 Units/g cellulase and 200 μm particle size. As concluded from the obtained data, not only the PKC-Cel was stable in the presence of the IL, also it was actually stable at a higher temperature than its optimum one. The reducing sugar obtained was 53.468±4.58 g/L which was equivalent to 0.3055 g reducing sugar/g EFB. This approach opens an insight for more studies in order to understand the actual effect of ILs on cellulases and their interactions in the aqueous system. It could also benefit in an efficient production of bioethanol from lignocellulosic biomass. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cellulase" title="cellulase">cellulase</a>, <a href="https://publications.waset.org/abstracts/search?q=hydrolysis" title=" hydrolysis"> hydrolysis</a>, <a href="https://publications.waset.org/abstracts/search?q=lignocellulose" title=" lignocellulose"> lignocellulose</a>, <a href="https://publications.waset.org/abstracts/search?q=pretreatment" title=" pretreatment"> pretreatment</a> </p> <a href="https://publications.waset.org/abstracts/50719/screening-of-factors-affecting-the-enzymatic-hydrolysis-of-empty-fruit-bunches-in-aqueous-ionic-liquid-and-locally-produced-cellulase-system" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/50719.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">365</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">6</span> Development of Broad Spectrum Nitrilase Biocatalysts and Bioprocesses for Nitrile Biotransformation</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Avinash%20Vellore%20Sunder">Avinash Vellore Sunder</a>, <a href="https://publications.waset.org/abstracts/search?q=Shikha%20Shah"> Shikha Shah</a>, <a href="https://publications.waset.org/abstracts/search?q=Pramod%20P.%20Wangikar"> Pramod P. Wangikar</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The enzymatic conversion of nitriles to carboxylic acids by nitrilases has gained significance in the green synthesis of several pharmaceutical precursors and fine chemicals. While nitrilases have been characterized from different sources, the industrial application requires the identification of nitrilases that possess higher substrate tolerance, wider specificity and better thermostability, along with the development of an efficient bioprocess for producing large amounts of nitrilase. To produce large amounts of nitrilase, we developed a fed-batch fermentation process on defined media for the high cell density cultivation of E. coli cells expressing the well-studied nitrilase from Alcaligenes fecalis. A DO-stat feeding approach was employed combined with an optimized post-induction strategy to achieve nitrilase titer of 2.5*105 U/l and 78 g/l dry cell weight. We also identified 16 novel nitrilase sequences from genome mining and analysis of substrate binding residues. The nitrilases were expressed in E. coli and their biocatalytic potential was evaluated on a panel of 22 industrially relevant nitrile substrates using high-throughput screening and HPLC analysis. Nine nitrilases were identified to exhibit high activity on structurally diverse nitriles including aliphatic and aromatic dinitriles, heterocyclic, -hydroxy and -keto nitriles. With fed-batch biotransformation, whole-cell Zobelia galactanivorans nitrilase achieved yields of 2.4 M nicotinic acid and 1.8 M isonicotinic acid from 3-cyanopyridine and 4-cyanopyridine respectively within 5 h, while Cupravidus necator nitrilase enantioselectively converted 740 mM mandelonitrile to (R)–mandelic acid. The nitrilase from Achromobacter insolitus could hydrolyze 542 mM iminodiacetonitrile in 1 h. The availability of highly active nitrilases along with bioprocesses for enzyme production expands the toolbox for industrial biocatalysis. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=biocatalysis" title="biocatalysis">biocatalysis</a>, <a href="https://publications.waset.org/abstracts/search?q=isonicotinic%20acid" title=" isonicotinic acid"> isonicotinic acid</a>, <a href="https://publications.waset.org/abstracts/search?q=iminodiacetic%20acid" title=" iminodiacetic acid"> iminodiacetic acid</a>, <a href="https://publications.waset.org/abstracts/search?q=mandelic%20acid" title=" mandelic acid"> mandelic acid</a>, <a href="https://publications.waset.org/abstracts/search?q=nitrilase" title=" nitrilase"> nitrilase</a> </p> <a href="https://publications.waset.org/abstracts/141515/development-of-broad-spectrum-nitrilase-biocatalysts-and-bioprocesses-for-nitrile-biotransformation" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/141515.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">234</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">5</span> Solitary Fibrous Tumor Presumed to Be a Peripheral Nerve Sheath Tumor Involving Right Branchial Plexus</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Daniela%20Proca">Daniela Proca</a>, <a href="https://publications.waset.org/abstracts/search?q=Yuan%20Rong"> Yuan Rong</a>, <a href="https://publications.waset.org/abstracts/search?q=Salvatore%20Luceno"> Salvatore Luceno</a>, <a href="https://publications.waset.org/abstracts/search?q=Jalil%20Nasibli"> Jalil Nasibli</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Introduction: Solitary Fibrous Tumors (SFT) have many histologic mimickers and the only way to diagnose it, particularly in an unusual location, such as peripheral nerve trunks, is to use a comprehensive immunohistochemical staining panel. Monoclonal STAT6 immunostain is highly sensitive and specific for SFTs and particularly useful in the diagnosis of difficult SFT cases. Methods: We describe a solitary fibrous tumor (SFT) involving the right branchial plexus in a 66 yo female with 4-year history of slowly growing chest wall mass with recent dysesthesias in fingers 4th and 5th. MRI showed a well-circumscribed heterogenous mass measuring 5.4 x 3.8 x 4.0 cm and encircling peripheral nerves of the branchial plexus; no involvement of the bone or muscle was noted. A biopsy showed a bland spindled and epithelioid proliferation with no significant mitotic activity, no necrosis, and no atypia; peripheral nerve fascicles were encircled by the lesion. The main clinical and pathologic differential diagnosis included peripheral nerve sheath tumor, particularly schwannoma; HE microscopy didn’t show the classic Antoni A and B areas but showed focal subtle nuclear palisading, as well as prominent vessels with hyalinization. Immunohistochemical stains showed focal, weak cytoplasmic S100 positivity in the lesion; CD 34 and Vimentin were strongly and diffusely positive; the neoplastic cells were negative with AE1/AE3, EMA, CD31, SMA, Desmin, Calretinin, HMB-45, Melan A, PAX-8, NSE. The immunohistochemical and histologic pattern was not typical of peripheral nerve sheath tumor. On additional stains, the tumor was positive with STAT-6 and bcl-2 and focally positive with CD99. Given this profile, the final diagnosis was that of a solitary fibrous tumor. Results: NA Conclusion: Very few SFTs involving peripheral nerves and mimicking a peripheral nerve sheath tumor are described in the literature. Although histologically benign on this biopsy, long-term follow-up is required because of the risk of recurrence of these tumors and their uncertain biological behavior. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=solitary%20fibrous%20tumor" title="solitary fibrous tumor">solitary fibrous tumor</a>, <a href="https://publications.waset.org/abstracts/search?q=pathology" title=" pathology"> pathology</a>, <a href="https://publications.waset.org/abstracts/search?q=diagnosis" title=" diagnosis"> diagnosis</a>, <a href="https://publications.waset.org/abstracts/search?q=immunohistochemistry" title=" immunohistochemistry"> immunohistochemistry</a> </p> <a href="https://publications.waset.org/abstracts/141452/solitary-fibrous-tumor-presumed-to-be-a-peripheral-nerve-sheath-tumor-involving-right-branchial-plexus" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/141452.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">196</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4</span> A Novel Application of CORDYCEPIN (Cordycepssinensis Extract): Maintaining Stem Cell Pluripotency and Improving iPS Generation Efficiency</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Shih-Ping%20Liu">Shih-Ping Liu</a>, <a href="https://publications.waset.org/abstracts/search?q=Cheng-Hsuan%20Chang"> Cheng-Hsuan Chang</a>, <a href="https://publications.waset.org/abstracts/search?q=Yu-Chuen%20Huang"> Yu-Chuen Huang</a>, <a href="https://publications.waset.org/abstracts/search?q=Shih-Yin%20Chen"> Shih-Yin Chen</a>, <a href="https://publications.waset.org/abstracts/search?q=Woei-Cherng%20Shyu"> Woei-Cherng Shyu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Embryonic stem cells (ES) and induced pluripotnet stem cells (iPS) are both pluripotent stem cells. For mouse stem cells culture technology, leukemia inhibitory factor (LIF) was used to maintain the pluripotency of stem cells in vitro. However, LIF is an expensive reagent. The goal of this study was to find out a pure compound extracted from Chinese herbal medicine that could maintain stem cells pluripotency to replace LIF and improve the iPS generation efficiency. From 20 candidates traditional Chinese medicine we found that Cordycepsmilitaris triggered the up-regulation of stem cells activating genes (Oct4 and Sox2) expression levels in MEF cells. Cordycepin, a major active component of Cordycepsmilitaris, also could up-regulate Oct4 and Sox2 gene expression. Furthermore, we used ES and iPS cells and treated them with different concentrations of Cordycepin (replaced LIF in the culture medium) to test whether it was useful to maintain the pluripotency. The results showed higher expression levels of several stem cells markers in 10 μM Cordycepin-treated ES and iPS cells compared to controls that did not contain LIF, including alkaline phosphatase, SSEA1, and Nanog. Embryonic body formation and differentiation confirmed that 10 μM Cordycepin-containing medium was capable to maintain stem cells pluripotency after four times passages. For mechanism analysis, microarray analysis indicated extracellular matrix and Jak/Stat signaling pathway as the top two deregulated pathways. In ECM pathway, we determined that the integrin αVβ5 expression levels and phosphorylated Src levels increased after Cordycepin treatment. In addition, the phosphorylated Jak2 and phosphorylated Sat3 protein levels were increased after Cordycepin treatment and suppressed with the Jak2 inhibitor, AG490. The expression of cytokines associated with Jak2/Stat3 signaling pathway were also up-regulated by Q-PCR and ELISA assay. Lastly, we used Oct4-GFP MEF cells to test iPS generation efficiency following Cordycepin treatment. We observed that 10 Μm Cordycepin significantly increased the iPS generation efficiency in day 21. In conclusion, we demonstrated Cordycepin could maintain the pluripotency of stem cells through both of ECM and Jak2/Stat3 signaling pathway and improved iPS generation efficiency. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cordycepin" title="cordycepin">cordycepin</a>, <a href="https://publications.waset.org/abstracts/search?q=iPS%20cells" title=" iPS cells"> iPS cells</a>, <a href="https://publications.waset.org/abstracts/search?q=Jak2%2FStat3%20signaling%20pathway" title=" Jak2/Stat3 signaling pathway"> Jak2/Stat3 signaling pathway</a>, <a href="https://publications.waset.org/abstracts/search?q=molecular%20biology" title=" molecular biology"> molecular biology</a> </p> <a href="https://publications.waset.org/abstracts/6862/a-novel-application-of-cordycepin-cordycepssinensis-extract-maintaining-stem-cell-pluripotency-and-improving-ips-generation-efficiency" class="btn btn-primary btn-sm">Procedia</a> <a 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