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Reconstruction of mandibular defects with autogenous bone and decellularized bovine bone grafts with freeze‑dried bone marrow stem cell paracrine factors
<!DOCTYPE html> <!--[if lt IE 7]> <html class="no-js lt-ie9 lt-ie8 lt-ie7"> <![endif]--> <!--[if IE 7]> <html class="no-js lt-ie9 lt-ie8"> <![endif]--> <!--[if IE 8]> <html class="no-js lt-ie9"> <![endif]--> <!--[if gt IE 8]> <html class="no-js"> <![endif]--> <!--[if gt IE 8]><!--> <html class="no-js" prefix="og: http://ogp.me/ns#"> <!--<![endif]--> <head> <title>Reconstruction of mandibular defects with autogenous bone and decellularized bovine bone grafts with freeze‑dried bone marrow stem cell paracrine factors</title> <meta http-equiv="content-type" content="text/html; charset=UTF-8" /> <meta charset="utf-8" /> <meta http-equiv="X-UA-Compatible" content="IE=edge,chrome=1" /> <meta name="citation_doi" content="10.3892/ol.2017.5647" /> <meta name="citation_journal_title" content="Oncology Letters" /> <meta name="citation_title" content="Reconstruction of mandibular defects with autogenous bone and decellularized bovine bone grafts with freeze‑dried bone marrow stem cell paracrine factors" /> <meta name="citation_xml_url" content="https://www.spandidos-publications.com/10.3892/ol.2017.5647/xml" /> <meta name="citation_author" content="Kakabadze,Ann" /> <meta name="citation_author_institution" content="Department of Clinical Anatomy, Tbilisi State Medical University, 0177 Tbilisi, Georgia" /> <meta name="citation_author" content="Mardaleishvili,Konstantine" /> <meta name="citation_author_institution" content="Department of Clinical Anatomy, Tbilisi State Medical University, 0177 Tbilisi, Georgia" /> <meta name="citation_author" content="Loladze,George" /> <meta name="citation_author_institution" content="Department of Surgery, Cancer Research Center, 0177 Tbilisi, Georgia" /> <meta name="citation_author" content="Karalashvili,Lia" /> <meta name="citation_author_institution" content="Department of Clinical Anatomy, Tbilisi State Medical University, 0177 Tbilisi, Georgia" /> <meta name="citation_author" content="Chutkerashvili,Gocha" /> <meta name="citation_author_institution" content="Department of Molecular and Translational Medicine, Institute of Medical Research, Ilia State University, 0177 Tbilisi, Georgia" /> <meta name="citation_author" content="Chakhunashvili,David" /> <meta name="citation_author_institution" content="Department of Clinical Anatomy, Tbilisi State Medical University, 0177 Tbilisi, Georgia" /> <meta name="citation_author" content="Kakabadze,Zurab" /> <meta name="citation_author_institution" content="Department of Clinical Anatomy, Tbilisi State Medical University, 0177 Tbilisi, Georgia" /> <meta name="citation_publication_date" content="2017-03-01" /> <meta name="citation_volume" content="13" /> <meta name="citation_issue" content="3" /> <meta name="citation_firstpage" content="1811" /> <meta name="citation_lastpage" content="1818" /> <meta name="citation_publisher" content="Spandidos Publications" /> <meta name="citation_issn" content="1792-1074" /> <meta name="citation_abstract_html_url" content="http://www.spandidos-publications.com/10.3892/ol.2017.5647/abstract" /> <meta name="citation_pdf_url" content="http://www.spandidos-publications.com/10.3892/ol.2017.5647/download" /> <meta name="citation_fulltext_world_readable" content="" /> <meta name="keywords" content="cancer, oncology, oncogene, molecular medicine, tumor, apoptosis, gene, cell, research, scientific journals, tumour suppressor genes, cell cycle, growth factors" /> <meta name="verify-v1" content="qIInQfvSmbVNPO1nn+hEr0GnchYI4j93np2wrWGr29A="></meta> <meta name="verify-v1" content="KOd/fzGN2I+RDbonO/qYB3yaji9QcOddBCxBKx+5j9U="></meta> <meta name="twitter:card" content="summary"></meta> <meta name="twitter:title" content="Reconstruction of mandibular defects with autogenous bone and decellularized bovine bone grafts with freeze‑dried bone marrow stem cell paracrine factors"></meta> <meta name="twitter:site" content="https://www.spandidos-publications.com/10.3892/ol.2017.5647" /> <meta name="twitter:image" content="https://www.spandidos-publications.com/resources/images/ol.jpg"/> <meta name="twitter:description" content="The gold standard following segmental mandibulectomy is vascularized autologous bone graft in the form of the fibula flap. However, in bone reconstruction the use of autogenous bone does not always guarantee a successful outcome. The aim of the present investigation was to develop a novel biologically active bone (BAB) graft, and to use it for the reconstruction of large size defects of the mandible bone following tumor resection. In the first part of the present study, biologically active bone graft was developed by using human freeze‑dried bone marrow stem cells (BMSCs) paracrine factors and three‑dimensional bone scaffold derived from cancellous bovine bone following decellularization. In the second part of the research, one male and three female patients with primary tumors of the mandible underwent hemimandibulectomy. The mandibular bone defects following tumor resection were reconstructed with autogenous rib grafts in three patients and BAB graft was used in one patient. The graft‑host interfaces were covered with decellularized human amnion/chorion membrane graft. All patients were followed‑up every five months following the reconstruction of the mandible, with no complications observed. Preliminary clinical investigations demonstrated that a BAB graft containing freeze‑dried BMSC paracrine factors may be used for the reconstruction of large mandibular bone defects following tumor resection."/> <meta property='og:title' content="Reconstruction of mandibular defects with autogenous bone and decellularized bovine bone grafts with freeze‑dried bone marrow stem cell paracrine factors"/> <meta property='og:image:secure_url' content="https://www.spandidos-publications.com/resources/images/ol.jpg"/> <meta name="image" property='og:image' content="https://www.spandidos-publications.com/resources/images/ol.jpg"/> <meta property="og:image:width" content="200" /> <meta property="og:image:height" content="200" /> <meta property="og:image:type" content="image/jpg" /> <meta property='og:description' content="The gold standard following segmental mandibulectomy is vascularized autologous bone graft in the form of the fibula flap. However, in bone reconstruction the use of autogenous bone does not always guarantee a successful outcome. The aim of the present investigation was to develop a novel biologically active bone (BAB) graft, and to use it for the reconstruction of large size defects of the mandible bone following tumor resection. In the first part of the present study, biologically active bone graft was developed by using human freeze‑dried bone marrow stem cells (BMSCs) paracrine factors and three‑dimensional bone scaffold derived from cancellous bovine bone following decellularization. In the second part of the research, one male and three female patients with primary tumors of the mandible underwent hemimandibulectomy. The mandibular bone defects following tumor resection were reconstructed with autogenous rib grafts in three patients and BAB graft was used in one patient. The graft‑host interfaces were covered with decellularized human amnion/chorion membrane graft. All patients were followed‑up every five months following the reconstruction of the mandible, with no complications observed. Preliminary clinical investigations demonstrated that a BAB graft containing freeze‑dried BMSC paracrine factors may be used for the reconstruction of large mandibular bone defects following tumor resection."/> <meta property='og:url' content="https://www.spandidos-publications.com/10.3892/ol.2017.5647"/> <link rel="stylesheet" type="text/css" media="all" href="/resources/css/styles.css" /> <link rel="stylesheet" href="/resources/css/jquery.fancybox.css" type="text/css" /> <!--[if IE 7]> <link rel="stylesheet" type="text/css" href="/resources/css/ie7.css"> <![endif]--> <script src="/resources/js/modernizr-3.3.1.min.js" type="text/javascript"></script> <!-- Google tag (gtag.js) --> <script async src="https://www.googletagmanager.com/gtag/js?id=G-8R96ZFDKY3"></script> <script> window.dataLayer = window.dataLayer || []; function gtag(){dataLayer.push(arguments);} gtag('js', new Date()); gtag('config', 'G-8R96ZFDKY3'); </script> </head> <body class="ol"> <!--[if lt IE 7]> <p class="chromeframe">You are using an outdated browser. <a href="http://browsehappy.com/">Upgrade your browser today</a> or <a href="http://www.google.com/chromeframe/?redirect=true">install Google Chrome Frame</a> to better experience this site.</p> <![endif]--> <div class="wrapper"> <div> <div class="super wide d-flex pb-4"> <div class="super fixed_width"> <ul > <li><a href="/home">Home</a></li> <li><a href="/osats">Submit Manuscript</a></li> <!-- <th:block th:if="${session != null and session.user != null and session.user.type == 'user'}"> <li th:if="${session.user.maxYearlyReviews} gt 0" ><a href="/reviews">Reviews</a></li> </th:block> --> <li><a href="/myaccount">My Account</a></li> </ul> </div> </div> <div class="header wide"> <div class="header fixed_width"> <div class="header_logo"> <a href="/home"><img alt="spandidos logo" src="/resources/images/spandidoslogo.png" width="275" height="89"/></a> </div> <div class="header_search"> <div id="searchbtn"> <a href="/advancedsearch/ol">Advanced Search</a> </div> <div class="searchbox"> <a href="javascript:BasicSearch('ol')" ><img alt="search button" src="/resources/images/searchbutton.png"/></a> <input class="" type="text" id= "searchFor" /> </div> <div> <ul > <li><a href="/register">Register</a></li> <li><a href="/login" id="loginLink">Login</a></li> </ul> </div> </div> <div class="header_nav"> <ul> <li style="width: 8.3%;" class="ol"><a href="/ol">Oncology <br />Letters</a></li> <li style="width: 8.3%;" class="ijo"><a href="/ijo">International <br />Journal <br />of Oncology</a></li> <li style="width: 8.3%;" class="mco"><a href="/mco">Molecular <br />and Clinical <br />Oncology</a></li> <li style="width: 8.3%;" class="etm"><a href="/etm">Experimental <br />and Therapeutic <br />Medicine</a></li> <li style="width: 8.3%;" class="ijmm"><a href="/ijmm">International <br />Journal <br />of Molecular <br />Medicine</a></li> <li style="width: 8.3%;" class="br"><a href="/br">Biomedical <br />Reports</a></li> <li style="width: 8.3%;" class="or"><a href="/or">Oncology <br />Reports</a></li> <li style="width: 8.3%;" class="mmr"><a href="/mmr">Molecular <br />Medicine <br />Reports</a></li> <li style="width: 8.3%;" class="wasj"><a href="/wasj">World Academy <br />of Sciences <br />Journal</a></li> <li style="width: 8.3%;" class="ijfn"><a href="/ijfn">International <br />Journal <br />of Functional<br />Nutrition</a></li> <li style="width: 8.3%;" class="ije"><a href="/ije">International <br />Journal <br />of Epigenetics</a></li> <li style="width: 8.3%;" class="mi"><a href="/mi">Medicine <br />International </a></li> </ul> </div> </div> </div> <!-- Journal and Article pages show Journal banner --> <div class="banner wide flex"> <div class="banner fixed_width"> <h1>Oncology <br/>Letters</h1> <img src="/resources/images/bannerol.jpg" alt="Journal Banner" /> </div> </div> </div> <div class="content wide"> <div class="content fixed_width"> <div class="content_sidebar"> <ul class="sidebar_journal_top"> <li class="sidebar_title"><a href="/ol">Journal Home</a></li> <li > <a href="/ol">Current Issue</a> </li> <li> <a href="/ol/forthcoming">Forthcoming Issue</a> </li> <li> <a id="toggleSpecialIssue" href="#" onclick="$('.toggleSpecialIssue').toggle();return false;">Special Issues</a> <ul class="toggleSpecialIssue" style="display:none; 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This is an open access article distributed under the terms of <a href="https://creativecommons.org/licenses/by-nc-nd/4.0/">Creative Commons Attribution</a> License. </p> </li> </ul> <div> <div id="metrics"> <div> <span title="The metrics are calculated only from this site" class="bold">Metrics: </span> <span class="small" title="The metrics are calculated only from this site">Total Views: </span> <span title="The metrics are calculated only from this site" class="bold small" id="postTotalFullArticleViews">0 </span> <span class="small">(Spandidos Publications:</span> <span class="bold small" id="postFullArticleViews"></span> <span class="small"> | PMC Statistics: </span> <span class="bold small" id="postPMCFullArticleViews"></span> <span class="small">)</span> <div> <span style="visibility: hidden;" title="The metrics are calculated only from this site" class="bold">Metrics: </span> <span title="The metrics are calculated only from this site" class="small">Total PDF Downloads: </span> <span title="The metrics are calculated only from this site" id="postTotalArticleDownloads" class="bold small">0 </span> <span class="small">(Spandidos Publications:</span> <span class="bold small" id="postArticleDownloads"></span> <span class="small"> | PMC Statistics: </span> <span class="bold small" id="postArticlePMCDownloads"></span> <span class="small">)</span> </div> </div> </div> <div> <span id="citationCountId"> <a class="citedByCountId" style="margin-left: 0px" href="/getTotalCount/10.3892/ol.2017.5647"></a> </span> <span id="citation"> <a class="citedBy" style="margin-left: 0px" href="/citationhtml/10.3892/ol.2017.5647/html"> Cited By (CrossRef): </a> </span> <span class="bold small" id="citationCount">0</span> <span class="bold small"> citations </span> <span class="bold small citationAllArticlesClass" id="citationAllArticlesId"> <a id="citedByIdViewArticles" class="citedBy" style="font-size: 15px; text-decoration: none; margin-left: 0px" href="/citationhtml/10.3892/ol.2017.5647/html"> Loading Articles... </a> </span> </div> <a class="moreLikeThis" style="display: none" href="/morelikethis/43447"></a> <div id="citationDiv" style="display: none"> <h4>This article is mentioned in:</h4> <div id="citations"></div> </div> <span class="__dimensions_badge_embed__" data-hide-zero-citations="true" data-legend="always" data-style="small_circle" data-doi="10.3892/ol.2017.5647"></span> <script async="async" src="https://badge.dimensions.ai/badge.js" charset="utf-8"></script> <br></br> <span data-hide-no-mentions="true" data-badge-details="right" class='altmetric-embed' data-badge-type="donut" data-link-target="_blank" data-doi="10.3892/ol.2017.5647"></span> </div> <h4>Abstract</h4> <div id = "articleAbstract">The gold standard following segmental mandibulectomy is vascularized autologous bone graft in the form of the fibula flap. However, in bone reconstruction the use of autogenous bone does not always guarantee a successful outcome. The aim of the present investigation was to develop a novel biologically active bone (BAB) graft, and to use it for the reconstruction of large size defects of the mandible bone following tumor resection. In the first part of the present study, biologically active bone graft was developed by using human freeze‑dried bone marrow stem cells (BMSCs) paracrine factors and three‑dimensional bone scaffold derived from cancellous bovine bone following decellularization. In the second part of the research, one male and three female patients with primary tumors of the mandible underwent hemimandibulectomy. The mandibular bone defects following tumor resection were reconstructed with autogenous rib grafts in three patients and BAB graft was used in one patient. The graft‑host interfaces were covered with decellularized human amnion/chorion membrane graft. All patients were followed‑up every five months following the reconstruction of the mandible, with no complications observed. Preliminary clinical investigations demonstrated that a BAB graft containing freeze‑dried BMSC paracrine factors may be used for the reconstruction of large mandibular bone defects following tumor resection.</div> <div> <div> <h4>Introduction</h4> <p>The gold standard following segmental mandibulectomy is vascularized autologous bone grafts in the form of the fibula flap (<a href="#b1-ol-0-0-5647" class="linksColor">1</a>,<a href="#b2-ol-0-0-5647" class="linksColor">2</a>). These autografts possess osteoinduction, osteoconduction and osseointegration properties. However, the use of autogenous bone does not necessarily guarantee a successful outcome in bone reconstruction. In cases of large bone defects, there are limits to the amount of cancellous bone grafts that can be harvested from a patient's bones (<a href="#b3-ol-0-0-5647" class="linksColor">3</a>). Autogenous bone grafts may also increase the risk of morbidity at the donor site (<a href="#b4-ol-0-0-5647" class="linksColor">4</a>,<a href="#b5-ol-0-0-5647" class="linksColor">5</a>).</p> <p>Considering this, there is an interest in allogeneic, xenogeneic and synthetic bone grafts for the reconstruction of bone defects. However, allogeneic and xenogeneic bone grafts have a risk of transmitting various diseases, the potential to develop incompatibility reactions and chronic granulomatous inflammation, and religious restrictions (<a href="#b6-ol-0-0-5647" class="linksColor">6</a>–<a href="#b8-ol-0-0-5647" class="linksColor">8</a>). The main advantage of synthetic bone grafts is their biocompatibility and bioresorption; however, they are unable to provide full bone regeneration. This is due to insufficient vascularization, weak osteoconductivity, inadequacy of bone formation, lower mechanical resistance and stability of the graft (<a href="#b9-ol-0-0-5647" class="linksColor">9</a>,<a href="#b10-ol-0-0-5647" class="linksColor">10</a>).</p> <p>In recent years, biological scaffold materials composed of extracellular matrix (ECM) have been successfully used for the remodeling of a variety of damaged soft tissues and bones, including those in the maxillofacial region (<a href="#b11-ol-0-0-5647" class="linksColor">11</a>–<a href="#b16-ol-0-0-5647" class="linksColor">16</a>). Tissue-specific ECM serves an important role in promoting tissue regeneration and repair. In order to enhance the osteointegration of bone ECM, bone marrow progenitor cells and platelet rich plasma may be used (<a href="#b17-ol-0-0-5647" class="linksColor">17</a>).</p> <p>Bone marrow stem cells (BMSCs) demonstrate excellent potential for use in cellular therapy and regenerative medicine (<a href="#b18-ol-0-0-5647" class="linksColor">18</a>). However, there is currently a growing interest in the process of preservation of various cells by lyophilization (<a href="#b19-ol-0-0-5647" class="linksColor">19</a>,<a href="#b20-ol-0-0-5647" class="linksColor">20</a>). BMSCs paracrine factors and their role in the process of damaged tissue and organ restoration are increasingly being studied (<a href="#b21-ol-0-0-5647" class="linksColor">21</a>–<a href="#b25-ol-0-0-5647" class="linksColor">25</a>).</p> <p>The present study hypothesized that a biologically active bone (BAB) graft may be developed by using freeze-dried BMSC paracrine factors and a three-dimensional bone scaffold created from bovine cancellous femoral bones. In the present study, the method for producing BAB grafts and the possibility of applying this product in patients needing reconstruction of large defects of mandibular bone following tumor resection is presented and discussed.</p> <h4>Materials and methods</h4> <h6>Patient information</h6> <p>A total of one male and three female patients (age range, 38–55 years) with primary tumors of the mandible, who underwent surgical treatment between January 2008 and December 2015 in the Cancer Research Center of Tbilisi, Georgia, were enrolled into the present study. All patients signed written informed consent for the present study, which was conducted according to the guidelines of the 1975 Declaration of Helsinki and approved by the Ethics Committee of the Cancer Research Centre in Tbilisi, Georgia.</p> <p>Patients underwent preoperative orthopantomography, bone scanning, computed tomography (CT) and magnetic resonance imaging. The lesions were biopsied, and the histopathological examination revealed osteoblastoma in three patients and osteoblastic osteosarcoma in one patient. All patients underwent hemimandibulectomy and following tumor resection, mandibular bone defects were reconstructed with autogenous rib grafts in three patients, and BAB graft was used in one patient. The graft-host interfaces were covered with decellularized human amnion/chorion membrane (dHACM) graft.</p> <h6>Fabrication of BAB grafts</h6> <p>Fresh samples of bovine femur bones were collected from a slaughterhouse (Tbilisi, Georgia) 3–4 h subsequent to slaughter. Following bacteriological analysis, the bone was separated from the soft tissue. The femur bones were rinsed in running water for 1 h and cut with a saw (JG210 Bone Cutting machine; Shandong China Coal Industrial & Mining Supplies Group Co., Ltd., Shandong, China) into bone fragments of 15×4×2 cm.</p> <p>Bone fragments were placed in deionized water solution containing 5,000 units/ml heparin (Sigma-Aldrich; EMD Millipore, Billerica, MA, USA) for 24 h to remove blood components present in the bone. Subsequently, the bone fragments were rinsed with 800 ml 0.9% saline solution and frozen at −80°C for at least 12 h (fragments were fully submerged in the solution). The frozen fragments of bone were thawed at 4°C and rinsed with phosphate-buffered saline (PBS; Sigma-Aldrich; EMD Millipore) prior to sequential washes in 0.01, 0.1 and 1% sodium dodecyl sulfate (SDS; Sigma-Aldrich; EMD Millipore) solution for 72 h. Bone fragments were rinsed with distilled H<span class="subscript">2</span>O for 45 min, followed by 1% Triton X-100 (Sigma-Aldrich; EMD Millipore) for 2 h to remove the remaining SDS. Following Triton X-100 treatment, the decellularized bone fragments were rinsed with PBS for 4 h.</p> <p>The scaffolds were placed in a stirrer and rinsed with a solution containing chloroform and ethanol, initially at a ratio of 2:1 for 24 h, then at a ratio of 1:2 for the following 24 h. To remove the remaining chloroform and ethanol, the scaffolds were placed in laboratory grade glass (600-ml PYREX™ Griffin Beakers; Thermo Fisher Scientific, Inc., Waltham, MA, USA), then deionized water (400 ml) was added, and the glass was placed on a compact digital mini rotator (#88880026; Thermo Fisher Scientific, Inc.), 37°C, with a gentle shaking speed of 50 rpm for 12 h. Subsequently, the bone fragments were rinsed in fresh deionized water for 2 h.</p> <p>Decellularized bone fragments (<a href="#f1-ol-0-0-5647" class="linksColor">Fig. 1A-D</a>) were placed in a stirrer and rinsed with 4% sodium hypochlorite for 24 h. To remove the remaining solvent from the deproteinized bone, fragments placed in laboratory grade glass (600-ml PYREX™ Griffin Beakers), deionized water (400 ml) was added, and the glass was placed on a compact digital mini rotator at 37°C, with a gentle shaking speed of 50 rpm, and left for 72 h.</p><div id="f1-ol-0-0-5647"><a class="fancybox" data-fancybox-group="figures" href="/article_images/ol/13/3/ol-13-03-1811-g00.jpg" title="Figure 1. - Bovine bone prior to and following
decellularization, lyophilization and seeding with human
freeze-dried bone marrow stem cells. (A) Stereoscopic microscope
image of native bovine trabecular bone (х16 magnification), (B)
decellularized bovine bone graft (х16 magnification) and (C) porous
frontal surface of the bone graft (х32 magnification). (D) Micro
computed tomography image of decellularized bone tissue (scale bar,
1 mm). (E) Stereoscopic microscope image of rear surface of
decellularized and lyophilized bovine bone graft placed in
RPMI-1640 medium (х32 magnification) and (F) bone marrow stem cells
seeded on the surface of the decellularized bone graft (х56
magnification). (G) Native and (H) decellularized bovine bone graft
stained with hematoxylin and eosin (magnification, ×400). (I)
Cluster of differentiation 105/endoglin (×100 magnification), (J)
bone morphogenetic protein-2, (×100 magnification) (K) collagen
type Iα1 (×100 magnification) and (L) fibronectin (×100
magnification) expression in biologically active bone grafts with
freeze-dried bone marrow stem cells."><div class="panel"><table><tbody><tr><td width="115px"><img class="panel-image" src="/article_images/ol/13/3/ol-13-03-1811-g00.jpg"></img></td><td><h4>Figure 1.</h4><p class="bold">Bovine bone prior to and following decellularization, lyophilization and seeding with human freeze-dried bone marrow stem cells. (A) Stereoscopic microscope image of native bovine trabecular bone (х16 magnification), (B) decellularized bovine bone graft (х16 magnification) and (C) porous frontal surface of the bone graft (х32 magnification). (D) Micro computed tomography image of decellularized bone tissue (scale bar, 1 mm). (E) Stereoscopic microscope image of rear surface of decellularized and lyophilized bovine bone graft placed in RPMI-1640 medium (х32 magnification) and (F) bone marrow stem cells seeded on the surface of the decellularized bone graft (х56 magnification). (G) Native and (H) decellularized bovine bone graft stained with hematoxylin and eosin (magnification, ×400). (I) Cluster of differentiation 105/endoglin (×100 magnification), (J) bone morphogenetic protein-2, (×100 magnification) (K) collagen type Iα1 (×100 magnification) and (L) fibronectin (×100 magnification) expression in biologically active bone grafts with freeze-dried bone marrow stem cells.</p></td></tr></tbody></table></div></a></div> <p>dHACM graft was acquired through a previously described decellularization protocol (<a href="#b26-ol-0-0-5647" class="linksColor">26</a>). The dHACM grafts that were created by using this method, following lyophilization, were packed in a disposable plastic bag and sterilized with gamma radiation (dose of 15 kGy). The dHACM grafts were stored aseptically at room temperature until use. Prior to the transplantation, the dHACM was rehydrated in a 0.9% saline solution for 30 min.</p> <h6>DNA quantification of BAB grafts</h6> <p>DNA was isolated from the BAB tissue in accordance with manufacturer's protocol using a commercial extraction kit (G-spin Total DNA Extraction Mini kit; iNtRON Biotechnology, Inc., Seongnam, South Korea). The total DNA was determined using a spectrophotometer (NanoDrop 1000; Thermo Fisher Scientific, Inc.) at 260 nm.</p> <h6>Collection of human autologous bone marrow stem cells</h6> <p>A total of 4 days prior to the reconstruction of mandibular defects, between 180–200 ml of bone marrow was aspirated from the patient's anterior iliac crest under local anesthesia, and placed in sterile tubes containing heparin. The aspirates were diluted 1:2 with PBS. The mononuclear fraction was isolated by density gradient centrifugation at 400 × g for 30 min at room temperature using Ficoll Paque Plus or Ficoll Paque Premium solution (GE Healthcare Bio-Sciences, Pittsburgh, PA, USA).</p> <h6>Flow cytometry and viability testing</h6> <p>The 0.5 ml final cell product was subjected to a trypan blue dye exclusion test and flow cytometric analysis. The viability test was performed using 0.4% trypan blue solution (Sigma-Aldrich; EMD Millipore), according to the manufacturer's protocol. For cell immunophenotyping, the cell suspensions were incubated with anti-human cluster of differentiation (CD) 45 FiTC/CD34 PE (dilution, 1:200; #647821; BD Biosciences, Franklin Lakes, NJ, USA), anti-human CD271 (dilution, 1:100; #560834; BD Biosciences) and anti-human stromal cell surface marker (STRO-1) antibodies (dilution, 1;100, #sc-47733; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), diluted in 0.5% bovine serum albumin/PBS (Sigma-Aldrich; EMD Millipore) buffer, according to the manufacturer's instructions. Flow cytometry analysis was performed on a BD FACSCalibur flow cytometer (BD Biosciences). Mononuclear CD45<span class="superscript">−</span>/CD34<span class="superscript">−</span>/CD271<span class="superscript">+</span>/STRO-1<span class="superscript">+</span> cells were defined as bone marrow mesenchymal stem cells, and their percentage and absolute count were recorded. Bone marrow hematopoietic stem cells were determined as the CD45<span class="superscript">+</span>/CD34<span class="superscript">+</span> mononuclear cell population.</p> <h6>Cell seeding</h6> <p>The BAB graft was thoroughly rinsed in sterile PBS for 30 min and immersed in RPMI-1640 medium (Sigma-Aldrich; EMD Millipore) so that only the bottom half of the graft was covered by the media.</p> <p>In total, 1.2×10<span class="superscript">8</span> mononuclear-enriched cells, suspended in 10 ml saline, were seeded on the top surface of the graft. After 15 min, the grafts were turned over, and the same quantity of cells was seeded on the opposite side. This process was repeated every 15 min for up to 1 h to facilitate uniform cell distribution (<a href="#f1-ol-0-0-5647" class="linksColor">Fig. 1E and F</a>). Decellularized bovine bone grafts with mononuclear enriched cells were cultivated in a humidified incubator (37°C, 5% CO<span class="subscript">2</span>) for 4 days. The differentiation medium was as follows: Dulbecco's modified Eagle's medium-low glucose (Sigma-Aldrich; EMD Millipore) supplemented with 10% fetal bovine serum (Sigma-Aldrich; EMD Millipore), 1 µM dexamethasone (Sigma-Aldrich; EMD Millipore), 50 µg/ml ascorbic acid (Sigma-Aldrich; EMD Millipore), 10 mM sodium β-glycerophosphate (Sigma-Aldrich; EMD Millipore) and 1% penicillin/streptomycin (Sigma-Aldrich; EMD Millipore). Subsequently, the bone graft with the seeded mononuclear-enriched cells was lyophilized.</p> <h6>Lyophilization protocol</h6> <p>The process of lyophilization consisted of two stages: Deep freezing of objects and thawing in a vacuum. Following seeding, the BAB graft with BMSCs complex was freeze-dried with a lyophilizer (Heto PowerDry PL6000 Freeze Dryer; Sjia Lab, Shenzhen, China). The temperature of the lyophilizer was set at −40°C, and the vacuum was controlled under 10–15 Pa. The thawing procedure lasted for 18–24 h. The chamber was warmed up to 15–20°C at a rate of 0.2°C/min and sustained for 6–8 h.</p> <p>Following lyophilization, the BAB grafts were packed in disposable plastic bags (Wipak Medical, Bomlitz, Germany), following which they were sterilized with gamma-ray doses of 15 kGy and stored in sterile conditions at room temperature until use.</p> <h6>Histology and fluorescence immunohistochemistry of BAB grafts containing freeze-dried BMSCs</h6> <p>Samples of bovine bone were harvested prior to and following decellularization, and were fixed in 10% neutral buffered formalin, embedded in paraffin, sectioned and stained with hematoxylin and eosin, and Masson's trichrome staining.</p> <p>Fluorescence immunohistochemistry was performed according to the following methods. For the staining of decellularized bovine bone grafts containing freeze-dried BMSCs with antibodies against CD105/endoglin, bone morphogenetic protein-2 (BMP-2), collagen, type I α1 (Iα1) and fibronectin, formalin-fixed paraffin-embedded tissue sections 5-µm thick were cut on a rotary microtome, mounted on charged slides and baked overnight at 50°C in an oven. All staining procedures were performed at room temperature.</p> <p>The slides were deparaffinized and rehydrated in water. Antigen retrieval was performed using steam and proteinase K digestion methods. Following antigen retrieval, the slides were allowed to cool at room temperature for 20 min, following which the slides were washed three times with PBS for 5 min each, and then blocked with 3% H<span class="subscript">2</span>O<span class="subscript">2</span>. Subsequently, the slides were incubated in the primary antibody: CD105/endoglin at 1:100 (#sc-19793; Santa Cruz Biotechnology, Inc.), BMP-2 at 1:100 (#sc-6895; Santa Cruz Biotechnology, Inc.) collagen Iα1 at 1:100 (#sc-25974; Santa Cruz Biotechnology, Inc.) and fibronectin at 1:200 (#sc-8422; Santa Cruz Biotechnology, Inc.) diluted with IHC-Tek Antibody Diluent (IHC World, LLC, Woodstock, MD, USA) for 1 h at room temperature. The slides were subsequently washed three times in PBS and incubated with a biotinylated secondary antibody at 1:800 dilution (anti-rabbit IgG; #B7389; Sigma-Aldrich; EMD Millipore) for 30 min. The slides were washed in PBS and incubated with horseradish peroxidase-conjugated streptavidin (#S2438; Sigma-Aldrich; EMD Millipore) for 30 min, prior to incubation with 3,3′-diaminobenzidine chromogen substrate solution (#D7304; Sigma-Aldrich; EMD Millipore) for 5–10 min, washing with PBS and counterstaining with Mayer's hematoxylin. Histological slides were analyzed by upright microscope (E100; Nikon Corporation, Tokyo, Japan) and bone fragment tissues by stereoscopic microscope (MBS 9; Lomo, St. Petersburg, Russia). Immunofluorescence staining was analyzed by fluorescence microscope (BH2-RFCA; Olympus Corporation, Tokyo, Japan).</p> <h6>MicroCT</h6> <p>MicroCT scanning was conducted in a µCT 50 compact cabinet microCT scanner (SCANCO Medical AG, Bassersdorf, Zurich, Switzerland) using a protocol previously described in detail by Zhu <span class="italic">et al</span> (<a href="#b27-ol-0-0-5647" class="linksColor">27</a>).</p> <h6>Scanning electron microscopy of BAB grafts containing freeze-dried BMSCs</h6> <p>The BAB grafts were dehydrated by processing with an ethanol solution prior to drying with a tousimis Samdri-780 Critical Point Dryer (Tousimis Research Corporation, Rockville, MD, USA). Following drying, all tissues were sputter coated lightly with gold and imaged on a Hitachi Scanning Electron microscope (Hitachi, Ltd., Tokyo, Japan).</p> <h6>Gene expression analysis of BAB grafts with freeze-dried BMSCs</h6> <p>The total RNA from the bone tissue was purified using a miRNeasy mini kit, according to the manufacturer's instructions (Qiagen GmbH, Hilden, Germany). cDNA was synthesized using the iScript cDNA synthesis kit (Bio-Rad Laboratories, Inc., Hercules, CA, USA). Quantitative polymerase chain reaction (qPCR) was performed using iTaq Universal SYBR Green supermix (Bio-Rad Laboratories, Inc.) and a 7500 Fast Real-Time PCR system (Thermo Fisher Scientific, Inc.). Thermal cycling conditions were as follows: 15 min of denaturation at 95°C, followed by 40 cycles of denaturation for 15 sec at 95°C, annealing for 30 sec at 60°C and elongation for 20 sec at 72°C. The 18S rRNA was used as an internal control for gene expression normalization, which is considered to be the most reliable reference gene for normalization of qPCR data (<a href="#b28-ol-0-0-5647" class="linksColor">28</a>).</p> <p>The polymerase chain reaction (PCR) amplification was performed using the following primer sets: BMP7 forward, 5′ACAGACCAAGCACCTCTCCT-3′ and reverse, 5′-CGGTGTGCTCAGGTTTCTAA-3′; BMP8a forward, 5′-ATTATGGTGGTCAGGGCATT-3′ and reverse, 5′-GCACCGTTATACCTGGCTCT-3′; epidermal growth factor (EGF) forward, 5′-ACTGGGAGCAGACAGAAGGT-3′ and reverse, 5′-ATTAGCCGTGGAGACAGGAG-3′; vascular endothelial growth factor (VEGF) forward, 5′-AGATTCTGCAAGAGCACCCT-3′ and reverse, 5′-CCTAGGCTCCTCAGAAGTGG-3′; fibroblast growth factor forward, 5′-ACCGGTACCTGGCTATGAAG-3′ and reverse, 5′-GTGCCACATACCAACTGGAG-3′; osteopontin forward, 5′-TCCAGGAGTTTCCCTGTTTC-3′ and reverse, 5′-TGACCTTGATAGCCTCATCG-3′; osteocalcin forward, 5′-CTGCATTCTGCCTCTCTGAC-3′ and reverse, 5′-CCGGAGTCTATTCACCACCT-3′; bone sialoprotein forward, 5′-GAGACAACGGAGAAGAAGCC-3′ and reverse, 5′-CCATAACTGGTCAGCTCCCT-3′; 18S rRNA forward, 5′-TAAAGGAATTGACGGAAGGG-3′ and reverse, 5′-CTGTCAATCCTGTCCGTGTC-3′ (<a href="#b29-ol-0-0-5647" class="linksColor">29</a>).</p> <h4>Results</h4> <h6>Characterization of BAB graft</h6> <p>Following decellularization, cancellous bone fragments acquired from bovine femur bones are a light yellow color; they have a desired three-dimensional porous structure and may be repopulated by host bone-forming cells (<a href="#b30-ol-0-0-5647" class="linksColor">30</a>). The DNA content of the fresh samples of bovine femur bones prior to treatment was 482 µg/ml. Following the decellularization procedure, the residual DNA content was <1.4%. Histological investigation indicated the presence of osteocytes in the native tissue, whereas in the decellularized tissues, the presence of cellular material was not observed (<a href="#f1-ol-0-0-5647" class="linksColor">Fig. 1G and H</a>). Following decellularization, the mesh of collagen fibers had retained their structure and was similar to natural bone. However, the collagen arrangements were disturbed, with gaps between the collagen fibers.</p> <p>A cross-section of the BAB graft following cell seeding indicated the presence of the cells at depth in the scaffolds, in addition to along the sides of the scaffolds. However, there were separate areas in the central regions of the BAB graft where the cells were not homogenously distributed. Immunohistochemical investigation demonstrated the presence of CD105/endoglin, BMP-2, collagen Iα1 and fibronectin in the BAB grafts containing freeze-dried BMSCs (<a href="#f1-ol-0-0-5647" class="linksColor">Fig. 1I-L</a>).</p> <p>Low magnification scanning electron micrographs demonstrated decellularized bovine bone, observed as a mesh of collagen fibers that were intact and which were similar to the structure of natural bone. Freeze-dried BMSCs (<a href="#f2-ol-0-0-5647" class="linksColor">Fig. 2A-D</a>) that were seeded on the BAB graft were scattered between the collagen fibers (<a href="#f2-ol-0-0-5647" class="linksColor">Fig. 2E-H</a>), and their arrangement was compact and orderly.</p><div id="f2-ol-0-0-5647"><a class="fancybox" data-fancybox-group="figures" href="/article_images/ol/13/3/ol-13-03-1811-g01.jpg" title="Figure 2. - Scanning electron microscopy of bone
graft and freeze-dried human bone marrow stem cells. (A and B)
Human bone marrow stem cell prior to (magnification, ×500 and
×2,000, respectively) and (C and D) following freeze-drying
(magnification, ×500 and ×2,000, respectively). (E and F) Scanning
electron micrograph of the bone graft (magnification ×1,000 and
×2,000, respectively). (G and H) Scanning electron micrograph of
bone marrow stem cells seeded on the decellularized bone graft
(magnification, ×500 and ×1,000, respectively)."><div class="panel"><table><tbody><tr><td width="115px"><img class="panel-image" src="/article_images/ol/13/3/ol-13-03-1811-g01.jpg"></img></td><td><h4>Figure 2.</h4><p class="bold">Scanning electron microscopy of bone graft and freeze-dried human bone marrow stem cells. (A and B) Human bone marrow stem cell prior to (magnification, ×500 and ×2,000, respectively) and (C and D) following freeze-drying (magnification, ×500 and ×2,000, respectively). (E and F) Scanning electron micrograph of the bone graft (magnification ×1,000 and ×2,000, respectively). (G and H) Scanning electron micrograph of bone marrow stem cells seeded on the decellularized bone graft (magnification, ×500 and ×1,000, respectively).</p></td></tr></tbody></table></div></a></div> <p>Gene expression analysis demonstrated that the BAB grafts containing freeze-dried BMSCs expressed a large number of varying growth factors, in particular osteocalcin and osteopontin, which may enhance the osteogenic process (<a href="#f3-ol-0-0-5647" class="linksColor">Fig. 3</a>).</p><div id="f3-ol-0-0-5647"><a class="fancybox" data-fancybox-group="figures" href="/article_images/ol/13/3/ol-13-03-1811-g02.jpg" title="Figure 3. - Relative gene expression levels in
isolated freeze-dried BMSCs and decellularized bovine bone grafts
seeded with freeze-dried BMSCs. Gene expression analysis
demonstrated that the BAB grafts containing freeze-dried BMSCs
expressed a large number of varying growth factors, in particular
osteocalcin and osteopontin. BMSCs, bone marrow stem cells; BAB,
biologically active bone; EGF, epidermal growth factor; FGF,
fibroblast growth factor; VEGF, vascular endothelial growth factor;
BMP, bone morphogenetic protein."><div class="panel"><table><tbody><tr><td width="115px"><img class="panel-image" src="/article_images/ol/13/3/ol-13-03-1811-g02.jpg"></img></td><td><h4>Figure 3.</h4><p class="bold">Relative gene expression levels in isolated freeze-dried BMSCs and decellularized bovine bone grafts seeded with freeze-dried BMSCs. Gene expression analysis demonstrated that the BAB grafts containing freeze-dried BMSCs expressed a large number of varying growth factors, in particular osteocalcin and osteopontin. BMSCs, bone marrow stem cells; BAB, biologically active bone; EGF, epidermal growth factor; FGF, fibroblast growth factor; VEGF, vascular endothelial growth factor; BMP, bone morphogenetic protein.</p></td></tr></tbody></table></div></a></div> <h6>Clinical data</h6> <p>Out of three female patients that were enrolled in the present study, two underwent right hemimandibulectomy and one underwent left hemimandibulectomy. The mandibular resections were all lateralized and none crossed the symphysis menti. Surgical defects were restored with autogenous rib grafts (<a href="#f4-ol-0-0-5647" class="linksColor">Fig. 4A and B</a>). Following the stabilization of the host mandible bone and autologous rib grafts with a titanium plate, the dHACM graft was placed around the defect (<a href="#f4-ol-0-0-5647" class="linksColor">Fig. 4C and D</a>). The dimensions of the dHACM graft were 10×4 cm, and placement ensured that a 2 cm portion of the graft overlapped with the intact portions beyond the proximal and distal defect interfaces. The dHACM graft was then secured in place (stretched in all directions) with a 2/0 vicryl suture with measures to assure that no vessels or muscles were included.</p><div id="f4-ol-0-0-5647"><a class="fancybox" data-fancybox-group="figures" href="/article_images/ol/13/3/ol-13-03-1811-g03.jpg" title="Figure 4. - Reconstruction of mandibular bone
defects with autogenous rib grafts and decellularized bovine bone
grafts seeded with freeze-dried bone marrow stem cell paracrine
factors. (A) X-ray of the patient diagnosed with osteoblastoma in
the right portion of the mandibular body. (B) Reconstruction of the
mandible with an autogenous VI rib graft. (C) Autogenous rib graft,
titanium plate and host mandibular bone covered with a
decellularized human amnion/chorion membrane graft. (D)
Postoperative X-ray after five months of follow-up. (E)
Postoperative X-ray following left hemimandibulectomy in a patient
with osteoblastic osteosarcoma. (F-H) Reconstruction of the
mandibular defect with a titanium plate and biologically active
bone graft seeded with freeze-dried bone marrow stem cell paracrine
factors. (I) Decellularized human amnion/chorion membrane graft
used to cover the defect. (J-M) Thoracodorsal flap on the vascular
pedicle was used for complete closure of the soft tissue without
tension. (N and O) Pre- and postoperative appearance following
mandibular reconstruction with the preserved contour of the
mandible and face. (P) X-ray image five months subsequent to
mandible bone defect reconstruction demonstrating bone volume
maintenance."><div class="panel"><table><tbody><tr><td width="115px"><img class="panel-image" src="/article_images/ol/13/3/ol-13-03-1811-g03.jpg"></img></td><td><h4>Figure 4.</h4><p class="bold">Reconstruction of mandibular bone defects with autogenous rib grafts and decellularized bovine bone grafts seeded with freeze-dried bone marrow stem cell paracrine factors. (A) X-ray of the patient diagnosed with osteoblastoma in the right portion of the mandibular body. (B) Reconstruction of the mandible with an autogenous VI rib graft. (C) Autogenous rib graft, titanium plate and host mandibular bone covered with a decellularized human amnion/chorion membrane graft. (D) Postoperative X-ray after five months of follow-up. (E) Postoperative X-ray following left hemimandibulectomy in a patient with osteoblastic osteosarcoma. (F-H) Reconstruction of the mandibular defect with a titanium plate and biologically active bone graft seeded with freeze-dried bone marrow stem cell paracrine factors. (I) Decellularized human amnion/chorion membrane graft used to cover the defect. (J-M) Thoracodorsal flap on the vascular pedicle was used for complete closure of the soft tissue without tension. (N and O) Pre- and postoperative appearance following mandibular reconstruction with the preserved contour of the mandible and face. (P) X-ray image five months subsequent to mandible bone defect reconstruction demonstrating bone volume maintenance.</p></td></tr></tbody></table></div></a></div> <p>In one male patient, aged 38 years, the solid tumor involved the whole of the left mandible, including the body and ramus. The lesion was biopsied which indicated osteoblastic osteosarcoma. The left hemimandible was removed en bloc with the tumor (<a href="#f4-ol-0-0-5647" class="linksColor">Fig. 4E</a>).</p> <p>Therefore, the initial plan to bridge the bony gap created with a titanium plate was not possible and the wound was closed primarily without reconstruction. Following 5 years of follow-up, complete remission was observed in this patient. For the reconstruction of the mandible bone defect, the BAB graft containing freeze-dried BMSC paracrine factors was selected (<a href="#f4-ol-0-0-5647" class="linksColor">Fig. 4F</a>). The titanium plate was used to stabilize the host bone (<a href="#f4-ol-0-0-5647" class="linksColor">Fig. 4G and H</a>), and additionally a dHACM wrap was used to cover the defect (<a href="#f4-ol-0-0-5647" class="linksColor">Fig. 4I</a>). The thoracodorsal flap on the vascular pedicle was used for the complete closure of the soft tissue without tension (<a href="#f4-ol-0-0-5647" class="linksColor">Fig. 4J-M</a>). The patient was followed-up every five months following the reconstruction of the mandible, and no complications were observed (<a href="#f4-ol-0-0-5647" class="linksColor">Fig. 4N and O</a>). A total of 5 months subsequent to mandible bone reconstruction, x-ray imaging demonstrated bone volume maintenance (<a href="#f4-ol-0-0-5647" class="linksColor">Fig. 4P</a>).</p> <h4>Discussion</h4> <p>Bones exhibit an excellent ability for healing via the natural mechanisms of bone repair (<a href="#b4-ol-0-0-5647" class="linksColor">4</a>). However, the healing process in patients following bone tumor resection may be slow or inadequate (<a href="#b31-ol-0-0-5647" class="linksColor">31</a>).</p> <p>Previous investigations showed that autogenous rib graft demonstrates a positive effect on the reconstruction of mandibular bone defects, as the autogenous grafts activate the mechanisms of bone formation, including osteoconduction, osteinduction and osteogenesis (<a href="#b32-ol-0-0-5647" class="linksColor">32</a>–<a href="#b35-ol-0-0-5647" class="linksColor">35</a>).</p> <p>However, in cases with large defects of the mandible, reconstruction with autogenous bone grafts represents a great challenge, as the structure and large size of the graft slows the revascularization process (<a href="#b36-ol-0-0-5647" class="linksColor">36</a>). A previous study reported non-vascularized graft failure in 17% of cases where the defect size was 6 cm, and in 75% of cases where the defect was ≥12 cm (<a href="#b37-ol-0-0-5647" class="linksColor">37</a>).</p> <p>Decellularized bone scaffolds have been considered as an alternative to autogenous grafts (<a href="#b38-ol-0-0-5647" class="linksColor">38</a>–<a href="#b40-ol-0-0-5647" class="linksColor">40</a>). The main drawback for these materials is that they have osteoinductive and osteoconductive characteristics; however, they lack the osteogenic properties that are present in autologous grafts (<a href="#b41-ol-0-0-5647" class="linksColor">41</a>). Following the seeding of freeze-dried BMSC paracrine factors on BAB grafts, which possess osteoinductive and osteoconductive features, these grafts additionally exhibited osteogenic properties. The complete absence or the presence of only minimal DNA following decellularization means BAB grafts are biocompatible and do not induce antigen-antibody reactions (<a href="#b41-ol-0-0-5647" class="linksColor">41</a>,<a href="#b42-ol-0-0-5647" class="linksColor">42</a>).</p> <p>Once implanted or injected, the BMSCs at the site of the lesion differentiate into several cell types (<a href="#b43-ol-0-0-5647" class="linksColor">43</a>,<a href="#b44-ol-0-0-5647" class="linksColor">44</a>). However, the mechanism of differentiation currently remains to be elucidated. Recently, BMSC paracrine factors and their role in the restoration of damaged tissues and organs have received increased interest (<a href="#b45-ol-0-0-5647" class="linksColor">45</a>–<a href="#b47-ol-0-0-5647" class="linksColor">47</a>).</p> <p>BMSC paracrine factors stimulate tissue regeneration via effects on homing, immunosuppression, differentiation, angiogenesis, stimulation of endogenous cells and potential regulation of specific metabolic signaling pathways (<a href="#b25-ol-0-0-5647" class="linksColor">25</a>). Following freeze-drying, BMSCs have been observed to retain >80% of paracrine factors, including VEGF-1, insulin-like growth factor 1, EGF, hepatocyte growth factor, keratinocyte growth factor, angiopoietin-1, stromal cell-derived factor-1, monocyte chemoattractant protein-1 and erythropoietin (<a href="#b24-ol-0-0-5647" class="linksColor">24</a>,<a href="#b48-ol-0-0-5647" class="linksColor">48</a>–<a href="#b50-ol-0-0-5647" class="linksColor">50</a>).</p> <p>In order to prevent the invasion of fibrous tissue between the autologous bone or BAB graft, and the host mandible bone, dHACM has been used as a barrier membrane. In examined cases in the present study, it was observed that using dHACM for the reconstruction of mandibular bone defects enhanced osseointegration and provided solid protection against fibrous tissue invasion between the bone graft and host mandible bone. Similar results have also been previously reported (<a href="#b51-ol-0-0-5647" class="linksColor">51</a>–<a href="#b55-ol-0-0-5647" class="linksColor">55</a>).</p> <p>In a previous study (<a href="#b26-ol-0-0-5647" class="linksColor">26</a>), the present authors demonstrated that the dHACM contained type III collagen, glycoproteins and numerous growth factors, including epidermal growth factor, basic fibroblast growth factor, keratinocyte growth factor, VEGF, TGFα, TGFβ, PDGF, hepatocyte growth factor and nerve growth factor. Following the decellularization of the dHACM, the majority of the growth factors and cytokines, and the structural and mechanical properties were preserved. In addition, dHACM is easy to prepare and handle, and by its use, fibrous tissue invasion can be prevented (<a href="#b51-ol-0-0-5647" class="linksColor">51</a>,<a href="#b56-ol-0-0-5647" class="linksColor">56</a>).</p> <p>Micromovements between the host mandibular bone and any type of donor bone graft prevent bone formation, due to fibrous tissue invasion (<a href="#b52-ol-0-0-5647" class="linksColor">52</a>,<a href="#b53-ol-0-0-5647" class="linksColor">53</a>). Non-rigidly fixed defects with no membrane showed ingrowths of fibroblasts and fibrous nonunions (<a href="#b54-ol-0-0-5647" class="linksColor">54</a>–<a href="#b56-ol-0-0-5647" class="linksColor">56</a>).</p> <p>In conclusion, BAB grafts seeded with freeze-dried BMSC paracrine factors hold great promise for the future of mandible defect reconstruction following tumor resection. As a result, this type of composite graft should be able negate the need for harvesting donor bone. 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