Preparation and Characterization of Chitosan Nanoparticles for Delivery of Oligonucleotides

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of Chitosan Nanoparticles for Delivery of Oligonucleotides</title> <meta name="description" content="Preparation and Characterization of Chitosan Nanoparticles for Delivery of Oligonucleotides"> <meta name="keywords" content="LMW-chitosan, chitosan nanoparticles, biocompatibility, cytotoxicity study, transfection efficiency, oligonucleotide"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" 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</div> <div class="card publication-listing mt-3 mb-3"> <h5 class="card-header" style="font-size:.9rem">Preparation and Characterization of Chitosan Nanoparticles for Delivery of Oligonucleotides</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Gyati%20Shilakari%20Asthana">Gyati Shilakari Asthana</a>, <a href="https://publications.waset.org/abstracts/search?q=Abhay%20Asthana"> Abhay Asthana</a>, <a href="https://publications.waset.org/abstracts/search?q=Dharm%20Veer%20Kohli"> Dharm Veer Kohli</a>, <a href="https://publications.waset.org/abstracts/search?q=Suresh%20Prasad%20Vyas"> Suresh Prasad Vyas </a> </p> <p class="card-text"><strong>Abstract:</strong></p> Purpose: The therapeutic potential of oligonucleotide (ODN) is primarily dependent upon its safe and efficient delivery to specific cells overcoming degradation and maximizing cellular uptake in vivo. The present study is focused to design low molecular weight chitosan nanoconstructs to meet the requirements of safe and effectual delivery of ODNs. LMW-chitosan is a biodegradable, water soluble, biocompatible polymer and is useful as a non-viral vector for gene delivery due to its better stability in water. Methods: LMW chitosan ODN nanoparticles (CHODN NPs) were formulated by self-assembled method using various N/P ratios (moles ratio of amine groups of CH to phosphate moieties of ODNs; 0.5:1, 1:1, 3:1, 5:1, and 7:1) of CH to ODN. The developed CHODN NPs were evaluated with respect to gel retardation assay, particle size, zeta potential and cytotoxicity and transfection efficiency. Results: Complete complexation of CH/ODN was achieved at the charge ratio of 0.5:1 or above and CHODN NPs displayed resistance against DNase I. On increasing the N/P ratio of CH/ODN, the particle size of the NPs decreased whereas zeta potential (ZV) value increased. No significant toxicity was observed at all CH concentrations. The transfection efficiency was increased on increasing N/P ratio from 1:1 to 3:1, whereas it was decreased with further increment in N/P ratio upto 7:1. Maximum transfection of CHODN NPs with both the cell lines (Raw 267.4 cells and Hela cells) was achieved at N/P ratio of 3:1. The results suggest that transfection efficiency of CHODN NPs is dependent on N/P ratio. Conclusion: Thus the present study states that LMW chitosan nanoparticulate carriers would be acceptable choice to improve transfection efficiency in vitro as well as in vivo delivery of oligonucleotide. <iframe src="https://publications.waset.org/abstracts/16384.pdf" style="width:100%; height:400px;" frameborder="0"></iframe> <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=LMW-chitosan" title="LMW-chitosan">LMW-chitosan</a>, <a href="https://publications.waset.org/abstracts/search?q=chitosan%20nanoparticles" title=" chitosan nanoparticles"> chitosan nanoparticles</a>, <a href="https://publications.waset.org/abstracts/search?q=biocompatibility" title=" biocompatibility"> biocompatibility</a>, <a href="https://publications.waset.org/abstracts/search?q=cytotoxicity%20study" title=" cytotoxicity study"> cytotoxicity study</a>, <a href="https://publications.waset.org/abstracts/search?q=transfection%20efficiency" title=" transfection efficiency"> transfection efficiency</a>, <a href="https://publications.waset.org/abstracts/search?q=oligonucleotide" title=" oligonucleotide"> oligonucleotide</a> </p> <a href="https://publications.waset.org/abstracts/16384/preparation-and-characterization-of-chitosan-nanoparticles-for-delivery-of-oligonucleotides" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/16384.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">849</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a 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Preparation and Characterization of Chitosan Nanoparticles for Delivery of Oligonucleotides