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glucosidase</title> <meta name="description" content="Search results for: alpha glucosidase"> <meta name="keywords" content="alpha glucosidase"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none 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action="https://publications.waset.org/abstracts/search"> <div id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="alpha glucosidase"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 791</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: alpha glucosidase</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">791</span> In Silico Study of Alpha glucosidase Inhibitors by Flavonoids</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Boukli%20Hacene%20Faiza">Boukli Hacene Faiza</a>, <a href="https://publications.waset.org/abstracts/search?q=Soufi%20Wassila"> Soufi Wassila</a>, <a href="https://publications.waset.org/abstracts/search?q=Ghalem%20Said"> Ghalem Said</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The oral antidiabetics drugs such as alpha glucosidase inhibitors present undesirable effects like acarbose. Flavonoids are class of molecules widely distributed in plants, for this reason we are interested in our work to study the inhibition in silico of alpha glucosidase by natural ligands ( flavonoids analogues) using molecular modeling methods using MOE (Molecular Operating Environment) software to predict their interaction with this enzyme with score energy, ADME /T tests and druglikeness properties experiments. Two flavonoids Beicalein and Apigenin have high binding affinity with alpha glucosidase with lower IC50 supposed potent inhibitors. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=alpha%20glucosidase" title="alpha glucosidase">alpha glucosidase</a>, <a href="https://publications.waset.org/abstracts/search?q=flavonoides%20analogues" title=" flavonoides analogues"> flavonoides analogues</a>, <a href="https://publications.waset.org/abstracts/search?q=drug%20research" title=" drug research"> drug research</a>, <a href="https://publications.waset.org/abstracts/search?q=molecular%20modeling" title=" molecular modeling"> molecular modeling</a> </p> <a href="https://publications.waset.org/abstracts/156715/in-silico-study-of-alpha-glucosidase-inhibitors-by-flavonoids" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/156715.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">107</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">790</span> Mechanism of in Vitro Inhibition of Alpha-Amylase, Alpha-Glucosidase by Ethanolic Extracts of Polyalthia Longifolia, Its in Vitro Cytotoxicity on L6, Vero Cell-Lines and Influence of Glucose Uptake by Rat Hemi-Diaphragm</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=P.%20Gayathri">P. Gayathri</a>, <a href="https://publications.waset.org/abstracts/search?q=G.%20P.%20Jeyanthi"> G. P. Jeyanthi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The bark of Polyalthia longifolia is used in ayurvedic system of medicine for the manangement of various ailments including diabetes mellitus. The bark of P. longifolia extracts was extracted using various polar and non-polar solvents and tested for inhibition of alpha-amylase and alpha-glucosidase among which the ethanolic extracts were found to be more potent. The ethanolic extracts of the bark were tested for the in vitro inhibition of alpha-amylase using starch as substrate and alpha-glucosidase using p-nitro phenyl alpha-D-gluco pyranoside as substrate to establish its in vitro antidiabetic effect. The mechanism of inhibition was determined by Dixon plot and Cornish-Bowden plot. The cytotoxic effect of the extract was tested on L6 and Vero cell-lines. The extract was partially purified by TLC. The individual effect of the ethanolic extract, TLC fractions and its combinatorial effect with insulin and glibenclamide on glucose uptake by rat hemi-diaphragm were studied.Results revealed that the ethanolic extracts of Polyalthia longifolia bark exhibited competitive inhibition of alpha-amylase and alpha-glucosidase. The extracts were also found not to be cytotoxic at the highest dose of 1 mg/mL. Glucose uptake study revealed that the extract alone and when combined with insulin, decreased the glucose uptake when compared to insulin control, however the purified TLC fractions exhibited significantly higher (p<0.05) glucose uptake by the rat hemi-diaphragm when compared to insulin. The study shows various possible mechanism of in vitro antidiabetic effect of the P. longifolia bark. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=alpha-amylase" title="alpha-amylase">alpha-amylase</a>, <a href="https://publications.waset.org/abstracts/search?q=alpha-glucosidase" title=" alpha-glucosidase"> alpha-glucosidase</a>, <a href="https://publications.waset.org/abstracts/search?q=dixon" title=" dixon"> dixon</a>, <a href="https://publications.waset.org/abstracts/search?q=cornish-bowden" title=" cornish-bowden"> cornish-bowden</a>, <a href="https://publications.waset.org/abstracts/search?q=L6" title=" L6 "> L6 </a>, <a href="https://publications.waset.org/abstracts/search?q=Vero%20cell-lines" title=" Vero cell-lines"> Vero cell-lines</a>, <a href="https://publications.waset.org/abstracts/search?q=glucose%20uptake" title=" glucose uptake"> glucose uptake</a>, <a href="https://publications.waset.org/abstracts/search?q=polyalthia%20longifolia%20bark" title=" polyalthia longifolia bark"> polyalthia longifolia bark</a>, <a href="https://publications.waset.org/abstracts/search?q=ethanolic%20extract" title=" ethanolic extract"> ethanolic extract</a>, <a href="https://publications.waset.org/abstracts/search?q=TLC%20fractions" title=" TLC fractions"> TLC fractions</a> </p> <a href="https://publications.waset.org/abstracts/34899/mechanism-of-in-vitro-inhibition-of-alpha-amylase-alpha-glucosidase-by-ethanolic-extracts-of-polyalthia-longifolia-its-in-vitro-cytotoxicity-on-l6-vero-cell-lines-and-influence-of-glucose-uptake-by-rat-hemi-diaphragm" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/34899.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">469</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">789</span> Inhibition of α-Glucosidase and Xanthine Oxidase by Curcumin and Its Analogs</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jung-Feng%20Hsieh">Jung-Feng Hsieh</a>, <a href="https://publications.waset.org/abstracts/search?q=Chu%20Ze%20Chen"> Chu Ze Chen</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Curcumin is the main active compound of turmeric that can inhibit the activities of α-glucosidase and xanthine oxidase (XO). α-Glucosidase and XO inhibitors are widely used to treat patients with diabetes mellitus and gout, respectively; therefore, the objective of this research was to evaluate the inhibitory activities of curcumin and its analogs against α-glucosidase and XO. Our results demonstrated that CM-F had the strongest antioxidant activity with a half-maximal effective concentration (EC50) of 9.39 ± 0.16 μM, which was superior to vitamin E (EC50=17.03 ± 0.09 μM). CM-F also exhibited potent inhibitory activity against XO with an IC50 value of 6.14 ± 0.38 μM and enzyme kinetic results revealed competitive inhibition of XO. We also found that CM-1 and CM-2 inhibited α-glucosidase with IC50 values of 21.06 ± 0.92 μM and 5.95 ± 0.09 μM, respectively, and kinetic studies indicated that both CM-1 and CM-2 are mixed competitive inhibitors of α-glucosidase. Furthermore, docking simulation identified five hydrogen bonds between XO and CM-F; however, only one and two hydrogen bonds are involved in CM-1 and CM-2 binding to α-glucosidase, respectively. Accordingly, curcumin and its analogs have the potential to be used in the treatment of patients with diabetes mellitus and gout. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=curcumin" title="curcumin">curcumin</a>, <a href="https://publications.waset.org/abstracts/search?q=%CE%B1-glucosidase" title=" α-glucosidase"> α-glucosidase</a>, <a href="https://publications.waset.org/abstracts/search?q=inhibitor" title=" inhibitor"> inhibitor</a>, <a href="https://publications.waset.org/abstracts/search?q=xanthine%20oxidase" title=" xanthine oxidase"> xanthine oxidase</a> </p> <a href="https://publications.waset.org/abstracts/80143/inhibition-of-a-glucosidase-and-xanthine-oxidase-by-curcumin-and-its-analogs" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/80143.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">204</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">788</span> Effects of Opuntia ficus-indica var. Saboten on Glucose Uptake and Insulin Sensitivity in Pancreatic β Cell</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Kang-Hyun%20Leem">Kang-Hyun Leem</a>, <a href="https://publications.waset.org/abstracts/search?q=Myung-Gyou%20Kim"> Myung-Gyou Kim</a>, <a href="https://publications.waset.org/abstracts/search?q=Hye%20Kyung%20Kim"> Hye Kyung Kim</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The prickly pear cactus (Opuntia ficus-indica) has a global distribution and have been used for medicinal benefits such as artherosclerosis, diabetes, gastritis, and hyperglycemia. However, very little information is currently available for their mechanism. The prikly pear variety Opuntia ficus-indica var. Saboten (OFS) is widely cultivated in Cheju Island, southwestern region of Korea, and used as a functional food. Present study investigated the effects of OFS on pancreatic β-cell function using pancreatic islet β cells (HIT cell). Alpha-glucosidase inhibition, glucose uptake, insulin secretion, insulin sensitivity, and pancreatic β cell proliferation were determined. The inhibitory effect of ethanol extract of OFS stem on α-glucosidase enzyme was measured in a cell free system. Glucose uptake was determined using fluorescent glucose analogue, 2-NBDG. Insulin secretion was measured by ELISA assay. Cell proliferation was measured by MTT assay. Ethanol extracts of OFS dose-dependently inhibited α-glucosidase activity as well as glucose uptake. Insulinotrophic effect of OFS extract was observed at high glucose media in pancreatic β-islet cells. Furthermore, pancreatic β cell regeneration was also observed.These results suggest that OFS mediates the antidiabetic activity mainly via α-glucosidase inhibition, glucose uptake, and improved insulin sensitivity. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=prickly%20pear%20cactus" title="prickly pear cactus">prickly pear cactus</a>, <a href="https://publications.waset.org/abstracts/search?q=Opuntia%20ficus-indica%20var.%20Saboten" title=" Opuntia ficus-indica var. Saboten"> Opuntia ficus-indica var. Saboten</a>, <a href="https://publications.waset.org/abstracts/search?q=pancreatic%20islet%20HIT%20cells" title=" pancreatic islet HIT cells"> pancreatic islet HIT cells</a>, <a href="https://publications.waset.org/abstracts/search?q=%CE%B1-glucosidase" title=" α-glucosidase"> α-glucosidase</a>, <a href="https://publications.waset.org/abstracts/search?q=glucose%20uptake" title=" glucose uptake"> glucose uptake</a>, <a href="https://publications.waset.org/abstracts/search?q=insulinotrophic" title=" insulinotrophic"> insulinotrophic</a> </p> <a href="https://publications.waset.org/abstracts/32210/effects-of-opuntia-ficus-indica-var-saboten-on-glucose-uptake-and-insulin-sensitivity-in-pancreatic-v-cell" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/32210.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">465</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">787</span> Investigating the Combined Medicinal Effects of Withania Somnifera (Ashwaghandha) and Murraya Koenigii (Curry Pata) in Vitro</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sadia%20Roshan">Sadia Roshan</a>, <a href="https://publications.waset.org/abstracts/search?q=Kulsoom%20Sughra"> Kulsoom Sughra</a>, <a href="https://publications.waset.org/abstracts/search?q=Shazia%20Shamas"> Shazia Shamas</a>, <a href="https://publications.waset.org/abstracts/search?q=Shamaila%20Irum"> Shamaila Irum</a>, <a href="https://publications.waset.org/abstracts/search?q=Haleema%20Sadia"> Haleema Sadia</a> </p> <p class="card-text"><strong>Abstract:</strong></p> To evaluate synergistic medicinal effects of Withania somnifera (Ashwaghandha) and Murraya koenigii (Curry pata) in vitro. Antimicrobial activity was determined using the disc diffusion method against five bacterial and two fungal strains. The antioxidant activity was evaluated by the DPPH assay. The antidiabetic activity was evaluated by alpha-glucosidase inhibition assay and alpha-amylase inhibition assay. Synergistic antibacterial activity was observed against all the strains of bacteria, either Gram-positive or Gram-negative and fungi under study conditions. The maximum antibacterial activity was displayed by combined extract against E. coli i.e. 26±0.4mm. Maximum antifungal activity was shown by combined extract against Aspergillus niger, i.e., 17.3±0.5mm. The antioxidant activity of the combined extract was also significant. Alpha-glucosidase inhibition and alpha-amylase inhibition assays also showed synergism. Results indicate that Withania somnifera and Murraya koengii have medicinal properties. The combined extract of both plants is more potent than their individual extracts, suggesting that these can work in synergism. The research suggests that different plant extracts could be used in combination to increase their medicinal activities by many folds, thus giving an insight into future use of herbal medication. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=withania%20somnifera" title="withania somnifera">withania somnifera</a>, <a href="https://publications.waset.org/abstracts/search?q=murraya%20koenigii" title=" murraya koenigii"> murraya koenigii</a>, <a href="https://publications.waset.org/abstracts/search?q=antimicrobial%20activity" title=" antimicrobial activity"> antimicrobial activity</a>, <a href="https://publications.waset.org/abstracts/search?q=gram-positive%20%20bacetria" title=" gram-positive bacetria"> gram-positive bacetria</a>, <a href="https://publications.waset.org/abstracts/search?q=gram-negative%20%20bacteria" title=" gram-negative bacteria"> gram-negative bacteria</a> </p> <a href="https://publications.waset.org/abstracts/182551/investigating-the-combined-medicinal-effects-of-withania-somnifera-ashwaghandha-and-murraya-koenigii-curry-pata-in-vitro" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/182551.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">79</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">786</span> The Role of Polyphenolic Compounds in the Alpha Amylase and Alpha Glucosidase Inhibitory Potentials of Extracts from the Leaves of Acalypha godseffiana from Eastern Nigeria: An in-vitro Study</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=A.%20K.%20Asekunowo">A. K. Asekunowo</a>, <a href="https://publications.waset.org/abstracts/search?q=A%20O.%20T.%20Asafa"> A O. T. Asafa</a>, <a href="https://publications.waset.org/abstracts/search?q=O.%20O.%20Okoh"> O. O. Okoh</a>, <a href="https://publications.waset.org/abstracts/search?q=O.%20T.%20Asekun"> O. T. Asekun</a>, <a href="https://publications.waset.org/abstracts/search?q=O.%20B.%20Familoni"> O. B. Familoni</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: Acalypha godseffiana is an important plant used both as an ornamental and herbs; its leaves are employed in management of diseases such as diabetics in Eastern Nigeria. Aim: The correlations of the polyphenolic compounds in the hypoglycemic potential of different extracts of leaves of A. godseffiana and their safety profile on cell lines were investigated. Materials and Methods: The phytochemical compositions and antioxidants potentials were determined using adopted methods. An in vitro approach was employed in determining the hypoglycemic potentials of the extracts on α-amylase and α-glucosidase. The Line weaver-Burke plot was used to evaluate the mechanisms of Inhibition mechanisms of the enzymes. Results and Conclusions: Antioxidants results revealed that total antioxidant capacity (TAC) of the acetone extract (IC50: 0.34 mg/mL) showed better activity compared to the standards (silymarine 0.52 mg/mL; gallic acid 0.51 mg/mL). In-vitro hypoglycemic activity of the extracts confirmed that acetone extract demonstrated strong and mild inhibitory potential against α-amylase and α-glucosidase respectively. The observed activity was concentration-dependent with IC50 values of 2.33 and 0.13 mg/mL. The observed hypoglycemic and anti-oxidant potentials of acetone extract A. godseffiana correlate to its high polyphenolic contents which include phenols (133.20 mg gallic acid g-1), flavonoid (350.60 mg quercetin g-1) and tannins (264.67 mg catechin g-1). The mechanisms of action exhibited by acetone extract of A. godseffiana were mixed non-competitive and uncompetitive; which can be attributed to its inhibitory properties on α-amylase and α-glucosidase respectively. This effect would cause reduction in the rate at which starch hydrolyse, boost palliated glucose levels; hence, making acetone extract of A. godseffiana a potential anti-hypoglycemic alternative. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Acalypha%20godeseffiana" title="Acalypha godeseffiana">Acalypha godeseffiana</a>, <a href="https://publications.waset.org/abstracts/search?q=acetone%20extract" title=" acetone extract"> acetone extract</a>, <a href="https://publications.waset.org/abstracts/search?q=anti-hypoglycemia" title=" anti-hypoglycemia"> anti-hypoglycemia</a>, <a href="https://publications.waset.org/abstracts/search?q=antioxidant" title=" antioxidant"> antioxidant</a>, <a href="https://publications.waset.org/abstracts/search?q=phytochemicals" title=" phytochemicals"> phytochemicals</a> </p> <a href="https://publications.waset.org/abstracts/61511/the-role-of-polyphenolic-compounds-in-the-alpha-amylase-and-alpha-glucosidase-inhibitory-potentials-of-extracts-from-the-leaves-of-acalypha-godseffiana-from-eastern-nigeria-an-in-vitro-study" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/61511.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">262</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">785</span> Effect of Spontaneous Ripening and Drying Techniques on the Bioactive Activities Peel of Plantain (Musa paradisiaca) Fruit</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Famuwagun%20A.%20A.">Famuwagun A. A.</a>, <a href="https://publications.waset.org/abstracts/search?q=Abiona%20O.%20O."> Abiona O. O.</a>, <a href="https://publications.waset.org/abstracts/search?q=Gbadamosi%20S.O."> Gbadamosi S.O.</a>, <a href="https://publications.waset.org/abstracts/search?q=Adeboye%20O.%20A."> Adeboye O. A.</a>, <a href="https://publications.waset.org/abstracts/search?q=Adebooye%20O.%20C."> Adebooye O. C.</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The need to provide more information on the perceived bioactive status of the peel of plantain fruit informed the design of this research. Matured Plantain fruits were harvested, and fruits were allowed to ripen spontaneously. Samples of plantain fruit were taken every fortnight, and the peels were removed. The peels were dried using two different drying techniques (Oven drying and sun drying) and milled into powdery forms. Other samples were picked and processed in a similar manner on the first, third, seventh and tenth day until the peels of the fruits were fully ripped, resulting in eight different samples. The anti-oxidative properties of the samples using different assays (DPPH, FRAP, MCA, HRSA, SRSA, ABTS, ORAC), inhibitory activities against enzymes related to diabetes (alpha-amylase and glucosidase) and inhibition against angiotensin-converting enzymes (ACE) were evaluated. The result showed that peels of plantain fruits on the 7th day of ripening and sundried exhibited greater inhibitions against free radicals, which enhanced its antioxidant activities, resulting in greater inhibitions against alpha-amylase and alpha-glucosidase enzymes. Also, oven oven-dried sample of the peel of plantain fruit on the 7th day of ripening had greater phenolic contents than the other samples, which also resulted in higher inhibition against angiotensin converting enzymes when compared with other samples. The results showed that even though the unripe peel of plantain fruit is assumed to contain excellent bioactive activities, consumption of the peel should be allowed to ripen for seven days after maturity and harvesting so as to derive maximum benefit from the peel. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=functional%20ingredient" title="functional ingredient">functional ingredient</a>, <a href="https://publications.waset.org/abstracts/search?q=diabetics" title=" diabetics"> diabetics</a>, <a href="https://publications.waset.org/abstracts/search?q=hypertension" title=" hypertension"> hypertension</a>, <a href="https://publications.waset.org/abstracts/search?q=functional%20foods" title=" functional foods"> functional foods</a> </p> <a href="https://publications.waset.org/abstracts/183949/effect-of-spontaneous-ripening-and-drying-techniques-on-the-bioactive-activities-peel-of-plantain-musa-paradisiaca-fruit" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/183949.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">51</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">784</span> Molecular Dynamics Simulation of Beta-Glucosidase of Streptomyces</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Adam%20Abate">Adam Abate</a>, <a href="https://publications.waset.org/abstracts/search?q=Elham%20Rasti"> Elham Rasti</a>, <a href="https://publications.waset.org/abstracts/search?q=Philip%20Romero"> Philip Romero </a> </p> <p class="card-text"><strong>Abstract:</strong></p> Beta-glucosidase is the key enzyme component present in cellulase and completes the final step during cellulose hydrolysis by converting the cellobiose to glucose. The regulatory properties of beta-glucosidases are most commonly found for the retaining and inverting enzymes. Hydrolysis of a glycoside typically occurs with general acid and general base assistance from two amino acid side chains, normally glutamic or aspartic acids. In order to obtain more detailed information on the dynamic events origination from the interaction with enzyme active site, we carried out molecular dynamics simulations of beta-glycosidase in protonated state (Glu-H178) and deprotonated state (Glu178). The theoretical models generated from our molecular dynamics simulations complement and advance the structural information currently available, leading to a more detailed understanding of Beta-glycosidase structure and function. This article presents the important role of Asn307 in enzyme activity of beta-glucosidase <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Beta-glucosidase" title="Beta-glucosidase">Beta-glucosidase</a>, <a href="https://publications.waset.org/abstracts/search?q=GROMACS" title=" GROMACS"> GROMACS</a>, <a href="https://publications.waset.org/abstracts/search?q=molecular%20dynamics%20simulation" title=" molecular dynamics simulation"> molecular dynamics simulation</a>, <a href="https://publications.waset.org/abstracts/search?q=structural%20parameters" title=" structural parameters "> structural parameters </a> </p> <a href="https://publications.waset.org/abstracts/31578/molecular-dynamics-simulation-of-beta-glucosidase-of-streptomyces" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/31578.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">398</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">783</span> The Influence of the Variety and Harvesting Date on Haskap Composition and Anti-Diabetic Properties</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Aruma%20Baduge%20Kithma%20Hansanee%20De%20Silva">Aruma Baduge Kithma Hansanee De Silva</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Haskap (Lonicera caerulea L.), also known as blue honeysuckle, is a recently commercialized berry crop in Canada. Haskap berries are rich in polyphenols, including anthocyanins, which are known for potential health-promoting effects. Cyanidin-3-O-glucoside (C3G) is the most prominent anthocyanin of haskap berries. Recent literature reveals the efficacy of C3G in reducing the risk of type 2 diabetes (T2D), which has become an increasingly common health issue around the world. The T2D is characterized as a metabolic disorder of hyperglycemia and insulin resistance. It has been demonstrated that C3G has anti-diabetic effects in various ways, including improvement in insulin sensitivity, and inhibition of activities of carbohydrate-hydrolyzing enzymes, including alpha-amylase and alpha-glucosidase. The goal of this study was to investigate the influence of variety and harvesting date on haskap composition, biological properties, and antidiabetic properties. The polyphenolic compounds present in four commercially grown haskap cultivars, Aurora, Rebecca, Larissa and Evie among five harvesting stages (H1-H5), were extracted separately in 80% ethanol and analyzed to characterize their phenolic profiles. The haskap berries contain different types of polyphenols including flavonoids and phenolic acids. Anthocyanin is the major type of flavonoid. C3G is the most prominent type of anthocyanin, which accounts for 79% of total anthocyanin in all extracts. The variety Larissa at H5 contained the highest average C3G content, and its ethanol extract had the highest (1212.3±63.9 mg/100g FW) while, Evie at H1 contained the lowest C3G content (96.9±40.4 mg/100g FW). The average C3G content of Larissa from H1 – H5 varies from 208 – 1212 mg/100g FW. Quarcetin-3-Rutinoside (Q3Rut) is the major type of flavonol and highest is observed in Rebecca at H4 (47.81 mg/100g FW). The haskap berries also contained phenolic acids, but approximately 95% of the phenolic acids consisted of chlorogenic acid. The cultivar Larissa has a higher level of anthocyanin than the other four cultivars. The highest total phenolic content is observed in Evie at H5 (2.97±1.03 mg/g DW) while the lowest in Rebecca at H1 (1.47±0.96 mg/g DW). The antioxidant capacity of Evie at H5 was higher (14.40±2.21 µmol TE/ g DW) among other cultivars and the lowest observed in Aurora at H3 (5.69±0.34 µmol TE/ g DW). Furthermore, Larissa H5 shows the greatest inhibition of carbohydrate-hydrolyzing enzymes including alpha-glucosidase and alpha-amylase. In conclusion Larissa, at H5 demonstrated highest polyphenol composition and antidiabetic properties. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=anthocyanin" title="anthocyanin">anthocyanin</a>, <a href="https://publications.waset.org/abstracts/search?q=cyanidin-3-O-glucoside" title=" cyanidin-3-O-glucoside"> cyanidin-3-O-glucoside</a>, <a href="https://publications.waset.org/abstracts/search?q=haskap" title=" haskap"> haskap</a>, <a href="https://publications.waset.org/abstracts/search?q=type%202%20diabetes" title=" type 2 diabetes"> type 2 diabetes</a> </p> <a href="https://publications.waset.org/abstracts/96371/the-influence-of-the-variety-and-harvesting-date-on-haskap-composition-and-anti-diabetic-properties" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/96371.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">459</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">782</span> Antidiabetic and Admet Pharmacokinetic Properties of Grewia Lasiocarpa E. Mey. Ex Harv. Stem Bark Extracts: An in Vitro and in Silico Study</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Akwu%20N.%20A.">Akwu N. A.</a>, <a href="https://publications.waset.org/abstracts/search?q=Naidoo%20Y."> Naidoo Y.</a>, <a href="https://publications.waset.org/abstracts/search?q=Salau%20V.%20F."> Salau V. F.</a>, <a href="https://publications.waset.org/abstracts/search?q=Olofinsan%20K.%20A."> Olofinsan K. A.</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Grewia lasiocarpa E. Mey. ex Harv. (Malvaceae) is a Southern African medicinal plant indigenously used with other plants for birthing problems. The anti-diabetic properties of the hexane, chloroform, and methanol extracts of Grewia lasiocarpa stem bark were assessed using in vitro α-glucosidase enzyme inhibition assay. The predictive in silico drug-likeness and toxicity properties of the phytocompounds were conducted using the pKCSM, ADMElab, and SwissADME computer-aided online tools. The highest α-glucosidase percentage inhibition was observed in the hexane extract (86.76%, IC50= 0.24 mg/mL), followed by chloroform (63.08%, IC50= 4.87 mg/mL) and methanol (53.22%, IC50= 9.41 mg/mL); while acarbose, the standard anti-diabetic drug was (84.54%, IC50= 1.96 mg/mL). The α-glucosidase assay revealed that the hexane extract exhibited the strongest carbohydrate inhibiting capacity and is a better inhibitor than the standard reference drug-acarbose. The computational studies also affirm the results observed in the in vitroα-glucosidaseassay. Thus, the extracts of G. lasiocarpa may be considered a potential plant-sourced compound for treating type 2 diabetes mellitus. This is the first study on the anti-diabetic properties of Grewia lasiocarpa hexane, chloroform, and methanol extracts using in vitro and in silico models. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=grewia%20lasiocarpa" title="grewia lasiocarpa">grewia lasiocarpa</a>, <a href="https://publications.waset.org/abstracts/search?q=%CE%B1-glucosidase%20inhibition" title=" α-glucosidase inhibition"> α-glucosidase inhibition</a>, <a href="https://publications.waset.org/abstracts/search?q=anti-diabetes" title=" anti-diabetes"> anti-diabetes</a>, <a href="https://publications.waset.org/abstracts/search?q=ADMET" title=" ADMET"> ADMET</a> </p> <a href="https://publications.waset.org/abstracts/151917/antidiabetic-and-admet-pharmacokinetic-properties-of-grewia-lasiocarpa-e-mey-ex-harv-stem-bark-extracts-an-in-vitro-and-in-silico-study" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/151917.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">104</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">781</span> Phytochemical and Biological Evaluation of Derris scandens</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Devarakonda%20Ramadevi">Devarakonda Ramadevi</a>, <a href="https://publications.waset.org/abstracts/search?q=Dasari%20Rambabu"> Dasari Rambabu</a>, <a href="https://publications.waset.org/abstracts/search?q=K.%20Suresh%20Babu"> K. Suresh Babu</a>, <a href="https://publications.waset.org/abstracts/search?q=Battu%20Ganga%20Rao"> Battu Ganga Rao</a>, <a href="https://publications.waset.org/abstracts/search?q=Lakshmi%20Sirisha%20Kotikalapudi"> Lakshmi Sirisha Kotikalapudi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The phytochemical and biological evaluation of the whole plant of Derris scandens is belonging to the family fabaceae. The dried plant of D.scandens was procured from the tirumala. The completely dried powder of the whole plant was taken and ground to a coarse powder which was then subjected to Soxhlet extraction with hexane and chloroform successively for 36 hrs. Chloroform extract was filtered and concentrated by using rotary evaporator an about 100g extract was obtained. The chloroform extract was subjected to column chromatographed over silicagel. From the column chromatography seven compounds were isolated named as osajin, scandinone, scandenone, 4,5,7-tri hydroxy biprenyl isoflavone, derris isoflavone-A, scandenin and isoscandinone. D.scandens resulting in the isolation of seven compounds in the plant was confirmed by spectral data (1H NMR, 13C NMR, ESI-MS and FTIR). The isolated compounds were screened for antioxidant activity, antidiabetic activity, α-glucosidase (inhibitory activity) and anti-bacterial activity. The isolated seven compounds were tested for α-glucosidase inhibitory activity and antioxidant activity. All the seven compounds showed good α-glucosidase inhibitory activity and moderate antioxidant activity. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Derris%20scandens" title="Derris scandens">Derris scandens</a>, <a href="https://publications.waset.org/abstracts/search?q=phytochemical" title=" phytochemical"> phytochemical</a>, <a href="https://publications.waset.org/abstracts/search?q=antioxident" title=" antioxident"> antioxident</a>, <a href="https://publications.waset.org/abstracts/search?q=antidiabetic" title=" antidiabetic"> antidiabetic</a>, <a href="https://publications.waset.org/abstracts/search?q=antibacterial%20activity" title=" antibacterial activity"> antibacterial activity</a> </p> <a href="https://publications.waset.org/abstracts/59490/phytochemical-and-biological-evaluation-of-derris-scandens" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/59490.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">316</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">780</span> The Effect of the Variety and Harvesting Date on Polyphenol Composition of Haskap (Lonicera caerulea L.) and Anti-diabetic Properties of Haskap Polyphenols</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Aruma%20Baduge%20Kithma%20De%20Silva">Aruma Baduge Kithma De Silva</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Haskap (Lonicera caerulea L.), also known as blue honeysuckle, is a newly commercialized berry crop in Canada. Haskap berries are rich in polyphenols, including, anthocyanins, which are known for potential health-promoting properties. Cyanidin-3-O-glucoside (C3G) is the most abundant anthocyanin of haskap berries. The compound C3G has the ability to reduce the risk of type 2 diabetes (T2D), which has become an increasingly common health issue around the world. The T2D is characterized as a metabolic disorder of hyperglycemia and insulin resistance. It has been demonstrated that C3G has anti-diabetic effects through several ways, including inhibition of dipeptidyl peptidase-4 (DPP-4), reduction of gluconeogenesis, improvement in insulin sensitivity, and inhibition of activities of carbohydrate hydrolyzing enzymes, including α-amylase and α-glucosidase. The goal of this study was to investigate the influence of variety and harvests maturity of haskap on C3G, other fruit quality characteristics and anti-diabetic activities of haskap berries using in vitro studies. The polyphenols present in four commercially grown haskap cultivars, Aurora, Rebecca, Larissa, and Evie harvested at five harvesting dates (H1-H5) apart from 2-3 days, were extracted separately. High-performance liquid chromatography electrospray ionization mass spectrometry (HPLC-ESI-MS) analyzes of polyphenols revealed that haskap berries contain predominantly anthocyanins, flavonols, flavan-3-ols, and phenolic acids. The compound C3G was the most prominent anthocyanin, which is available in approximately 79% of total anthocyanin in four cultivars. The Larissa at H5 contained the highest C3G content. The antioxidant capacity of Evie at H5 was greater than other cultivars. Furthermore, Larissa H5 showed the greatest inhibition of carbohydrate hydrolyzing enzymes including alpha-glucosidase and alpha-amylase. In conclusion, the haskap variety and harvesting date influenced the polyphenol composition and biological properties. The variety Larissa, at H5 harvesting date, contained the highest polyphenol content and the ability of inhibition of the carbohydrate hydrolyzing enzyme as well as DPP4 enzyme in order to reduce type 2 diabetes. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=anthocyanin" title="anthocyanin">anthocyanin</a>, <a href="https://publications.waset.org/abstracts/search?q=Haskap" title=" Haskap"> Haskap</a>, <a href="https://publications.waset.org/abstracts/search?q=type%202%20diabetes" title=" type 2 diabetes"> type 2 diabetes</a>, <a href="https://publications.waset.org/abstracts/search?q=polyphenol" title=" polyphenol"> polyphenol</a> </p> <a href="https://publications.waset.org/abstracts/99613/the-effect-of-the-variety-and-harvesting-date-on-polyphenol-composition-of-haskap-lonicera-caerulea-l-and-anti-diabetic-properties-of-haskap-polyphenols" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/99613.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">142</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">779</span> Development of Alpha Spectroscopy Method with Solid State Nuclear Track Detector Using Aluminium Thin Films</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Nidal%20Dwaikat">Nidal Dwaikat</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This work presents the development of alpha spectroscopy method with Solid-state nuclear track detectors using aluminum thin films. The resolution of this method is high, and it is able to discriminate between alpha particles at different incident energy. It can measure the exact number of alpha particles at specific energy without needing a calibration of alpha track diameter versus alpha energy. This method was tested by using Cf-252 alpha standard source at energies 5.11 Mev, 3.86 MeV and 2.7 MeV, which produced by the variation of detector -standard source distance. On front side, two detectors were covered with two Aluminum thin films and the third detector was kept uncovered. The thickness of Aluminum thin films was selected carefully (using SRIM 2013) such that one of the films will block the lower two alpha particles (3.86 MeV and 2.7 MeV) and the alpha particles at higher energy (5.11 Mev) can penetrate the film and reach the detector’s surface. The second thin film will block alpha particles at lower energy of 2.7 MeV and allow alpha particles at higher two energies (5.11 Mev and 3.86 MeV) to penetrate and produce tracks. For uncovered detector, alpha particles at three different energies can produce tracks on it. For quality assurance and accuracy, the detectors were mounted on thick enough copper substrates to block exposure from the backside. The tracks on the first detector are due to alpha particles at energy of 5.11 MeV. The difference between the tracks number on the first detector and the tracks number on the second detector is due to alpha particles at energy of 3.8 MeV. Finally, by subtracting the tracks number on the second detector from the tracks number on the third detector (uncovered), we can find the tracks number due to alpha particles at energy 2.7 MeV. After knowing the efficiency calibration factor, we can exactly calculate the activity of standard source. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=aluminium%20thin%20film" title="aluminium thin film">aluminium thin film</a>, <a href="https://publications.waset.org/abstracts/search?q=alpha%20particles" title=" alpha particles"> alpha particles</a>, <a href="https://publications.waset.org/abstracts/search?q=copper%20substrate" title=" copper substrate"> copper substrate</a>, <a href="https://publications.waset.org/abstracts/search?q=CR-39%20detector" title=" CR-39 detector"> CR-39 detector</a> </p> <a href="https://publications.waset.org/abstracts/41596/development-of-alpha-spectroscopy-method-with-solid-state-nuclear-track-detector-using-aluminium-thin-films" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/41596.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">365</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">778</span> Phytochemicals from Enantia Chlorantha Stem Bark Inhibits the Activity ?-Amylase and ?-Glucosidase: Molecular Docking Studies</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Hammed%20Tanimowo%20Aiyelabegan">Hammed Tanimowo Aiyelabegan</a>, <a href="https://publications.waset.org/abstracts/search?q=Oluchukwu%20Franklin%20Aladi"> Oluchukwu Franklin Aladi</a>, <a href="https://publications.waset.org/abstracts/search?q=Mutiu%20Adewumi%20Alabi"> Mutiu Adewumi Alabi</a>, <a href="https://publications.waset.org/abstracts/search?q=Raliat%20Abimbola%20Aladodo"> Raliat Abimbola Aladodo</a>, <a href="https://publications.waset.org/abstracts/search?q=Emmanuel%20Oladipupo%20Ajani"> Emmanuel Oladipupo Ajani</a>, <a href="https://publications.waset.org/abstracts/search?q=Abdulganiyu%20Giwa"> Abdulganiyu Giwa</a>, <a href="https://publications.waset.org/abstracts/search?q=Esther%20Owolabi"> Esther Owolabi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The study aimed to evaluate the inhibitory activities of ligands from Enantia chlorantha stem bark on α-amylase and α-glucosidase. In silico pharmacokinetic properties and docking scores were employed to analyse the inhibition using SwissADME and Autodock4.2, respectively. Results revealed that drug-likeness, pharmacokinetics and bioavailability radar of all the ligands except jatrorrhizine and acarbose falls within the radar according to the Lipinski rule of 5. The binding energies of the protein-ligand interactions also show that the ligand fits into the active site. The results obtained from this study show that the chemical constituents from Enantia chlorantha stem bark may bring about positive physiological changes in a patient suffering from diabetes mellitus. Further in vitro studies on diabetes cell lines and in vivo studies on the animal may validate these compounds for diabetes treatment. These phytoconstituents could help in the development of novel anti-diabetic molecules. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=diabetes%20mellitus" title="diabetes mellitus">diabetes mellitus</a>, <a href="https://publications.waset.org/abstracts/search?q=%3F-amylase" title=" ?-amylase"> ?-amylase</a>, <a href="https://publications.waset.org/abstracts/search?q=%3F-glucosidase" title=" ?-glucosidase"> ?-glucosidase</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20silico" title=" in silico"> in silico</a>, <a href="https://publications.waset.org/abstracts/search?q=Enantia%20chlorantha%20stem%20bark" title=" Enantia chlorantha stem bark"> Enantia chlorantha stem bark</a> </p> <a href="https://publications.waset.org/abstracts/145916/phytochemicals-from-enantia-chlorantha-stem-bark-inhibits-the-activity-amylase-and-glucosidase-molecular-docking-studies" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/145916.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">173</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">777</span> Design Manufacture and Testing of a Combined Alpha-Beta Double Piston Stirling Engine</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=A.%20Calvin%20Antony">A. Calvin Antony</a>, <a href="https://publications.waset.org/abstracts/search?q=Sakthi%20Kumar%20Arul%20Prakash"> Sakthi Kumar Arul Prakash</a>, <a href="https://publications.waset.org/abstracts/search?q=V.%20R.%20Sanal%20Kumar"> V. R. Sanal Kumar</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In this paper a unique alpha-beta double piston 'stirling engine' is designed, manufactured and conducted laboratory test to ameliorate the efficiency of the stirling engine. The paper focuses on alpha and beta type engines, capturing their benefits and eradicating their short comings; along with the output observed from the flywheel. In this model alpha engine is kinematically with a piston cylinder arrangement which works quite like a beta engine. The piston of the new cylinder is so designed that it replicates a glued displacer and power piston as similar to that of beta engine. The bigger part of the piston is the power piston, which has a gap around it, while the smaller part of the piston is tightly fit in the cylinder and acts like the displacer piston. We observed that the alpha-beta double piston stirling engine produces 25% increase in power compare to a conventional alpha stirling engine. This working model is a pointer towards for the design and development of an alpha-beta double piston Stirling engine for industrial applications for producing electricity from the heat producing exhaust gases. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=alpha-beta%20double%20piston%20stirling%20engine" title="alpha-beta double piston stirling engine ">alpha-beta double piston stirling engine </a>, <a href="https://publications.waset.org/abstracts/search?q=alpha%20stirling%20engine" title=" alpha stirling engine "> alpha stirling engine </a>, <a href="https://publications.waset.org/abstracts/search?q=beta%20double%20piston%20stirling%20engine" title=" beta double piston stirling engine "> beta double piston stirling engine </a>, <a href="https://publications.waset.org/abstracts/search?q=electricity%20from%20stirling%20engine" title=" electricity from stirling engine"> electricity from stirling engine</a> </p> <a href="https://publications.waset.org/abstracts/35104/design-manufacture-and-testing-of-a-combined-alpha-beta-double-piston-stirling-engine" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/35104.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">533</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">776</span> Biological Activity of Bilberry Pomace</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Gordana%20S.%20%C4%86etkovi%C4%87">Gordana S. Ćetković</a>, <a href="https://publications.waset.org/abstracts/search?q=Vesna%20T.%20Tumbas%20%C5%A0aponjac"> Vesna T. Tumbas Šaponjac</a>, <a href="https://publications.waset.org/abstracts/search?q=Sonja%20M.%20Djilas"> Sonja M. Djilas</a>, <a href="https://publications.waset.org/abstracts/search?q=Jasna%20M.%20%C4%8Canadanovi%C4%87-Brunet"> Jasna M. Čanadanović-Brunet</a>, <a href="https://publications.waset.org/abstracts/search?q=Sladjana%20M.%20Staj%C4%8Di%C4%87"> Sladjana M. Stajčić</a>, <a href="https://publications.waset.org/abstracts/search?q=Jelena%20J.%20Vuli%C4%87"> Jelena J. Vulić</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Bilberry is one of the most important dietary sources of phenolic compounds, including anthocyanins, phenolic acids, flavonol glycosides and flavan-3-ols. These phytochemicals have different biological activities and therefore may improve our health condition. Also, anthocyanins are interesting to the food industry as colourants. In the present study, bilberry pomace, a by-product of juice processing, was used as a potential source of bioactive compounds. The contents of total phenolic acids, flavonoids and anthocyanins in bilberry pomace were determined by HPLC/UV-Vis. The biological activities of bilberry pomace were evaluated by reducing power (RP) and α-glucosidase inhibitory potential (α-GIP), and expressed as RP0.5 value (the effective concentration of bilberry pomace extract assigned at 0.5 value of absorption) and IC50 value (the concentration of bilberry pomace extract necessary to inhibit 50% of α-glucosidase enzyme activity). Total phenolic acids content was 807.12 ± 25.16 mg/100 g pomace, flavonoids 54.36 ± 1.83mg/100 g pomace and anthocyanins 3426.18 ± 112.09 mg/100 g pomace. The RP0.5 value of bilberry pomace was 0.38 ± 0.02 mg/ml, while IC50 value was 1.82 ± 0.11 mg/ml. These results have revealed the potential for valorization of bilberry juice production by-products for further industrial use as a rich source of bioactive compounds and natural colourants (mainly anthocyanins). <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bilberry%20pomace" title="bilberry pomace">bilberry pomace</a>, <a href="https://publications.waset.org/abstracts/search?q=phenolics" title=" phenolics"> phenolics</a>, <a href="https://publications.waset.org/abstracts/search?q=antioxidant%20activity" title=" antioxidant activity"> antioxidant activity</a>, <a href="https://publications.waset.org/abstracts/search?q=reducing%20power" title=" reducing power"> reducing power</a>, <a href="https://publications.waset.org/abstracts/search?q=%CE%B1-glucosidase%20enzyme%20activity" title=" α-glucosidase enzyme activity"> α-glucosidase enzyme activity</a> </p> <a href="https://publications.waset.org/abstracts/21890/biological-activity-of-bilberry-pomace" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/21890.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">599</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">775</span> Antioxidant, Hypoglycemic and Hypotensive Effects Affected by Various Molecular Weights of Cold Water Extract from Pleurotus Citrinopileatus</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Pao-Huei%20Chen">Pao-Huei Chen</a>, <a href="https://publications.waset.org/abstracts/search?q=Shu-Mei%20Lin"> Shu-Mei Lin</a>, <a href="https://publications.waset.org/abstracts/search?q=Yih-Ming%20Weng"> Yih-Ming Weng</a>, <a href="https://publications.waset.org/abstracts/search?q=Zer-Ran%20Yu"> Zer-Ran Yu</a>, <a href="https://publications.waset.org/abstracts/search?q=Be-Jen%20Wang"> Be-Jen Wang</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Pancreatic α-amylase and intestinal α-glucosidase are the critical enzymes for the breakdown of complex carbohydrates into di- or mono-saccharide, which play an important role in modulating postprandial blood sugars. Angiotensin converting enzyme (ACE) converts inactive angiotensin-I into active angiotensin-II, which subsequently increase blood pressure through triggering vasoconstriction and aldosterone secretion. Thus, inhibition of carbohydrate-digestion enzymes and ACE will help the management of blood glucose and blood pressure, respectively. Studies showed Pleurotus citrinopileatus (PC), an edible mushroom and commonly cultured in oriental countries, exerted anticancer, immune improving, antioxidative, hypoglycemic and hypolipidemic effects. Previous studies also showed various molecular weights (MW) fractioned from extracts may affect biological activities due to varying contents of bioactive components. Thus, the objective of this study is to investigate the in vitro antioxidant, hypoglycemic and hypotenstive effects and distribution of active compounds of various MWs of cold water extract from P. citrinopileatus (CWEPC). CWEPC was fractioned into four various MW fractions, PC-I (＜1 kDa), PC-II (1-3.5 kDa), PC-III (3.5-10 kDa), and PC-IV (＞10 kDa), using an ultrafiltration system. The physiological activities, including antioxidant activities, the inhibition capabilities of pancreatic α-amylase, intestinal α-glucosidase, and hypertension-linked ACE, and the active components, including polysaccharides, protein, and phenolic contents, of CWEPC and four fractions were determined. The results showed that fractions with lower MW exerted a higher antioxidant activity (p<0.05), which was positively correlated to the levels of total phenols. In contrast, the inhibition effects on the activities of α-amylase, α-glucosidase, and ACE of PC-IV fraction were significantly higher than CWEPC and the other three low MW fractions (< 10 kDa), which was more related to protein contents. The inhibition capability of CWEPC and PC-IV on α-amylase activity was 1/13.4 to 1/2.7 relative to that of acarbose (positive control), respectively. However, the inhibitory ability of PC-IV on α-glucosidase (IC50 = 0.5 mg/mL) was significantly higher than acarbose (IC50 = 1.7 mg/mL). Kinetic data revealed that PC-IV fraction followed a non-competitive inhibition on α-glucosidase activity. In conclusion, the distribution of various bioactive components contribute to the functions of different MW fractions on oxidative stress prevention, and blood pressure and glucose modulation. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=%CE%B1-Amylase" title="α-Amylase">α-Amylase</a>, <a href="https://publications.waset.org/abstracts/search?q=angiotensin%20converting%20enzyme" title=" angiotensin converting enzyme"> angiotensin converting enzyme</a>, <a href="https://publications.waset.org/abstracts/search?q=%CE%B1-Glucosidase" title=" α-Glucosidase"> α-Glucosidase</a>, <a href="https://publications.waset.org/abstracts/search?q=Pleurotus%20citrinopileatus" title=" Pleurotus citrinopileatus"> Pleurotus citrinopileatus</a> </p> <a href="https://publications.waset.org/abstracts/25984/antioxidant-hypoglycemic-and-hypotensive-effects-affected-by-various-molecular-weights-of-cold-water-extract-from-pleurotus-citrinopileatus" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/25984.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">460</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">774</span> Mathematical Modelling of the Effect of Glucose on Pancreatic Alpha-Cell Activity </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Karen%20K.%20Perez-Ramirez">Karen K. Perez-Ramirez</a>, <a href="https://publications.waset.org/abstracts/search?q=Genevieve%20Dupont"> Genevieve Dupont</a>, <a href="https://publications.waset.org/abstracts/search?q=Virginia%20Gonzalez-Velez"> Virginia Gonzalez-Velez</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Pancreatic alpha-cells participate on glucose regulation together with beta cells. They release glucagon hormone when glucose level is low to stimulate gluconeogenesis from the liver. As other excitable cells, alpha cells generate Ca2+ and metabolic oscillations when they are stimulated. It is known that the glucose level can trigger or silence this activity although it is not clear how this occurs in normal and diabetic people. In this work, we propose an electric-metabolic mathematical model implemented in Matlab to study the effect of different glucose levels on the electrical response and Ca2+ oscillations of an alpha cell. Our results show that Ca2+ oscillations appear in opposite phase with metabolic oscillations in a window of glucose values. The model also predicts a direct relationship between the level of glucose and the intracellular adenine nucleotides showing a self-regulating pathway for the alpha cell. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ca2%2B%20oscillations" title="Ca2+ oscillations">Ca2+ oscillations</a>, <a href="https://publications.waset.org/abstracts/search?q=mathematical%20model" title=" mathematical model"> mathematical model</a>, <a href="https://publications.waset.org/abstracts/search?q=metabolic%20oscillations" title=" metabolic oscillations"> metabolic oscillations</a>, <a href="https://publications.waset.org/abstracts/search?q=pancreatic%20alpha%20cell" title=" pancreatic alpha cell"> pancreatic alpha cell</a> </p> <a href="https://publications.waset.org/abstracts/96002/mathematical-modelling-of-the-effect-of-glucose-on-pancreatic-alpha-cell-activity" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/96002.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">178</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">773</span> Structure, Bioinformatics Analysis and Substrate Specificity of a 6-Phospho-β-Glucosidase Glycoside Hydrolase 1 Enzyme from Bacillus licheniformis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Wayde%20Veldman">Wayde Veldman</a>, <a href="https://publications.waset.org/abstracts/search?q=Ozlem%20T.%20Bishop"> Ozlem T. Bishop</a>, <a href="https://publications.waset.org/abstracts/search?q=Igor%20Polikarpov"> Igor Polikarpov</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In bacteria, mono and disaccharides are phosphorylated during uptake into the cell via the widely used phosphoenolpyruvate (PEP)-dependent phosphotransferase transport system. As an initial step in the phosphorylated disaccharide metabolism pathway, certain glycoside hydrolase family 1 (GH1) enzymes play a crucial role in releasing phosphorylated and non-phosphorylated monosaccharides. However, structural determinants for the specificity of these enzymes still need to be clarified. GH1 enzymes are known to have a wide array of functions. According to the CAZy database, there are twenty-one different enzymatic activities in the GH1 family. Here, the structure and substrate specificity of a GH1 enzyme from Bacillus licheniformis, hereafter known as BlBglH, was investigated. The sequence of the enzyme BlBglH was compared to the sequences of other characterized GH1 enzymes using sequence alignment, sequence identity calculations, phylogenetic analysis, and motif discovery. Through these various analyses, BlBglH was found to have sequence features characteristic of the 6-phospho-β-glucosidase activity enzymes. Additionally, motif and structure comparisons of the three most commonly studied GH1 enzyme-activities revealed a shared loop amongst the different structures that consist of different sequence motifs – this loop is thought to guide specific substrates (depending on activity) towards the active-site. To further affirm BlBglH enzyme activity, molecular docking and molecular dynamics simulations were performed. Docking was carried out using 6-phospho-β-glucosidase enzyme-activity positive (p-Nitrophenyl-beta-D-glucoside-6-phosphate) and negative (p-Nitrophenyl-beta-D-galactoside-6-phosphate) control ligands, followed by 400 ns molecular dynamics simulations. The positive-control ligand maintained favourable interactions within the active site until the end of the simulation. The negative-control ligand was observed exiting the enzyme at 287 ns. Binding free energy calculations showed that the positive-control complex had a substantially more favourable binding energy compared to the negative-control complex. Jointly, the findings of this study suggest that the BlBglH enzyme possesses 6-phospho-β-glucosidase enzymatic activity. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=6-P-%CE%B2-glucosidase" title="6-P-β-glucosidase">6-P-β-glucosidase</a>, <a href="https://publications.waset.org/abstracts/search?q=glycoside%20hydrolase%201" title=" glycoside hydrolase 1"> glycoside hydrolase 1</a>, <a href="https://publications.waset.org/abstracts/search?q=molecular%20dynamics" title=" molecular dynamics"> molecular dynamics</a>, <a href="https://publications.waset.org/abstracts/search?q=sequence%20analysis" title=" sequence analysis"> sequence analysis</a>, <a href="https://publications.waset.org/abstracts/search?q=substrate%20specificity" title=" substrate specificity"> substrate specificity</a> </p> <a href="https://publications.waset.org/abstracts/120700/structure-bioinformatics-analysis-and-substrate-specificity-of-a-6-phospho-v-glucosidase-glycoside-hydrolase-1-enzyme-from-bacillus-licheniformis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/120700.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">130</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">772</span> Application of EEG Wavelet Power to Prediction of Antidepressant Treatment Response</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Dorota%20Witkowska">Dorota Witkowska</a>, <a href="https://publications.waset.org/abstracts/search?q=Pawe%C5%82%20Gosek"> Paweł Gosek</a>, <a href="https://publications.waset.org/abstracts/search?q=Lukasz%20Swiecicki"> Lukasz Swiecicki</a>, <a href="https://publications.waset.org/abstracts/search?q=Wojciech%20Jernajczyk"> Wojciech Jernajczyk</a>, <a href="https://publications.waset.org/abstracts/search?q=Bruce%20J.%20West"> Bruce J. West</a>, <a href="https://publications.waset.org/abstracts/search?q=Miroslaw%20Latka"> Miroslaw Latka</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In clinical practice, the selection of an antidepressant often degrades to lengthy trial-and-error. In this work we employ a normalized wavelet power of alpha waves as a biomarker of antidepressant treatment response. This novel EEG metric takes into account both non-stationarity and intersubject variability of alpha waves. We recorded resting, 19-channel EEG (closed eyes) in 22 inpatients suffering from unipolar (UD, n=10) or bipolar (BD, n=12) depression. The EEG measurement was done at the end of the short washout period which followed previously unsuccessful pharmacotherapy. The normalized alpha wavelet power of 11 responders was markedly different than that of 11 nonresponders at several, mostly temporoparietal sites. Using the prediction of treatment response based on the normalized alpha wavelet power, we achieved 81.8% sensitivity and 81.8% specificity for channel T4. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=alpha%20waves" title="alpha waves">alpha waves</a>, <a href="https://publications.waset.org/abstracts/search?q=antidepressant" title=" antidepressant"> antidepressant</a>, <a href="https://publications.waset.org/abstracts/search?q=treatment%20outcome" title=" treatment outcome"> treatment outcome</a>, <a href="https://publications.waset.org/abstracts/search?q=wavelet" title=" wavelet"> wavelet</a> </p> <a href="https://publications.waset.org/abstracts/2686/application-of-eeg-wavelet-power-to-prediction-of-antidepressant-treatment-response" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/2686.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">315</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">771</span> Assessing Digestive Enzymes Inhibitory Properties of Anthocyanins and Procyanidins from Apple, Red Grape, Cinnamon</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Pinar%20Ercan">Pinar Ercan</a>, <a href="https://publications.waset.org/abstracts/search?q=Sedef%20N.%20El"> Sedef N. El</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The goals of this study were to determine the total anthocyanin and procyanidin contents and their in vitro bioaccessibilities of apple, red grape and cinnamon by a static in vitro digestion method reported by the COST FA1005 Action INFOGEST, as well as in vitro inhibitory effects of these food samples on starch and lipid digestive enzymes. While the highest total anthocyanin content was found in red grape (164.76 ± 2.51 mg/100 g), the highest procyanidin content was found in cinnamon (6432.54±177.31 mg/100 g) among the selected food samples (p<0.05). The anthocyanin bioaccessibilities were found as 10.23±1 %, 8.23±0.64 %, and 8.73±0.70 % in apple, red grape, and cinnamon, respectively. The procyanidin bioaccessibilities of apple, red grape, and cinnamon were found as 17.57±0.71 %, 14.08±0.74 % and 18.75±1.49 %, respectively. The analyzed apple, red grape and cinnamon showed the inhibitory activity against α-glucosidase (IC50 544.27±21.94, 445.63±15.67, 1592±17.58 μg/mL, respectively), α-amylase (IC50 38.41±7.26, 56.12±3.60, 3.54±0.86 μg/mL, respectively), and lipase (IC50 52.65±2.05, 581.70±54.14, 49.63±2.72 μg/mL, respectively). Red grape sample showed the highest inhibitory activity against α-glucosidase, cinnamon showed the highest inhibitory activity against α-amylase and lipase according to IC50 (concentration of inhibitor required to produce a 50% inhibition of the initial rate of reaction) and Catechin equivalent inhibition capacity (CEIC50) values. This study reported that apple, grape and cinnamon samples can inhibit the activity of digestive enzymes in vitro. The consumption of these samples would be used in conjunction with a low-calorie diet for body weight management. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=anthocyanin" title="anthocyanin">anthocyanin</a>, <a href="https://publications.waset.org/abstracts/search?q=%CE%B1-amylase" title=" α-amylase"> α-amylase</a>, <a href="https://publications.waset.org/abstracts/search?q=%CE%B1-glucosidase" title=" α-glucosidase"> α-glucosidase</a>, <a href="https://publications.waset.org/abstracts/search?q=lipase" title=" lipase"> lipase</a>, <a href="https://publications.waset.org/abstracts/search?q=procyanidin" title=" procyanidin"> procyanidin</a> </p> <a href="https://publications.waset.org/abstracts/81744/assessing-digestive-enzymes-inhibitory-properties-of-anthocyanins-and-procyanidins-from-apple-red-grape-cinnamon" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/81744.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">181</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">770</span> Cloning, Expression and N-Terminal Pegylation of Human Interferon Alpha-2b Analogs and Their Cytotoxic Evaluation against Cancer Cell Lines</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Syeda%20Kiran%20Shahzadi">Syeda Kiran Shahzadi</a>, <a href="https://publications.waset.org/abstracts/search?q=Nasir%20Mahmood"> Nasir Mahmood</a>, <a href="https://publications.waset.org/abstracts/search?q=Muhammad%20Abdul%20Qadir"> Muhammad Abdul Qadir</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In the current research, three recombinant human interferon alpha-2b proteins (two modified and one normal form) were produced and Pegylated with an aim to produce more effective drugs against viral infections and cancers. The modified recombinant human interferon alpha-2b proteins were produced by site-directed modifications of interferon alpha 2b gene, targeting the amino acids at positions ‘R23’ and ‘H34’. The resulting chemically modified and unmodified forms of human interferon alpha 2b were conjugated with methoxy-polyethylene glycol propanealdehyde (400 KDa) and methoxy-polyethylene glycol succinimidyl succinate (400 KDa). Pegylation of normal and modified forms of Interferon alpha-2b prolong their release time and enhance their efficacy. The conjugation of PEG with modified and unmodified human interferon alpha 2b protein drugs was also characterized with 1H-NMR, HPLC, and SDS-PAGE. Antiproliferative assays of modified and unmodified forms of drugs were performed in cell based bioassays using MDBK cell lines. The results indicated that experimentally produced recombinant human interferon alpha-2b proteins were biologically active and resulted in significant inhibition of cell growth. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=protein%20refolding" title="protein refolding">protein refolding</a>, <a href="https://publications.waset.org/abstracts/search?q=antiproliferative%20activities" title=" antiproliferative activities"> antiproliferative activities</a>, <a href="https://publications.waset.org/abstracts/search?q=biomedical%20applications" title=" biomedical applications"> biomedical applications</a>, <a href="https://publications.waset.org/abstracts/search?q=human%20interferon%20alpha-2b" title=" human interferon alpha-2b"> human interferon alpha-2b</a>, <a href="https://publications.waset.org/abstracts/search?q=pegylation" title=" pegylation"> pegylation</a>, <a href="https://publications.waset.org/abstracts/search?q=mPEG-propionaldehyde" title=" mPEG-propionaldehyde"> mPEG-propionaldehyde</a>, <a href="https://publications.waset.org/abstracts/search?q=site%20directed%20mutagenesis" title=" site directed mutagenesis"> site directed mutagenesis</a>, <a href="https://publications.waset.org/abstracts/search?q=E.%20coli%20expression" title=" E. coli expression"> E. coli expression</a> </p> <a href="https://publications.waset.org/abstracts/83956/cloning-expression-and-n-terminal-pegylation-of-human-interferon-alpha-2b-analogs-and-their-cytotoxic-evaluation-against-cancer-cell-lines" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/83956.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">177</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">769</span> Fatty Acid Composition, Total Sugar Content and Anti-Diabetic Activity of Methanol and Water Extracts of Nine Different Fruit Tree Leaves Collected from Mediterranean Region of Turkey</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sengul%20Uysal">Sengul Uysal</a>, <a href="https://publications.waset.org/abstracts/search?q=Gokhan%20Zengin"> Gokhan Zengin</a>, <a href="https://publications.waset.org/abstracts/search?q=Abdurrahman%20Aktumsek"> Abdurrahman Aktumsek</a>, <a href="https://publications.waset.org/abstracts/search?q=Sukru%20Karatas"> Sukru Karatas</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In this research, we determined the total sugar content, fatty acid compositions and α-amylase and α-glucosidase inhibitory activity of methanolic and water extracts of nine different fruit tree leaves. α-amylase and α-glycosidase inhibitory activity were determined by using Caraway-Somogyi–iodine/potassium iodide (IKI) and 4-nitrophenyl-α-D-glucopyranoside (PNPG) as substrate, respectively. Total sugar content of the nine different fruit tree leaves varies from 281.02 mg GE/g (glucose equivalents) to 643.96 mg GE/g. Methanolic extract from avocado leaves had the strongest in α-amylase and α-glucosidase inhibitory activity, 69.21% and 96.26 %, respectively. Fatty acid composition of nine fruit tree leaves was characterized by GC (gas chromatography) and twenty-four components were identified. Among the tested fruit tree leaves, the main component was linolenic acid (49.09%). The level of essential fatty acids are over 50% in mulberry, grape and loquat leaves. PUFAs (polyunsaturated fatty acids) were major group of fatty acids present in oils of mulberry, fig, pomegranate, grape, and loquat leaves. Therefore, these oils can be considered as a good source of polyunsaturated fatty acids. Furthermore, avocado can be regarded as a new source for diabetic therapies. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=fatty%20acid%20compositions" title="fatty acid compositions">fatty acid compositions</a>, <a href="https://publications.waset.org/abstracts/search?q=total%20sugar%20contents" title=" total sugar contents"> total sugar contents</a>, <a href="https://publications.waset.org/abstracts/search?q=%CE%B1-amylase" title=" α-amylase"> α-amylase</a>, <a href="https://publications.waset.org/abstracts/search?q=%CE%B1-glucosidase" title=" α-glucosidase"> α-glucosidase</a>, <a href="https://publications.waset.org/abstracts/search?q=fruit%20tree%20leaves" title=" fruit tree leaves"> fruit tree leaves</a>, <a href="https://publications.waset.org/abstracts/search?q=Turkey" title=" Turkey"> Turkey</a> </p> <a href="https://publications.waset.org/abstracts/19405/fatty-acid-composition-total-sugar-content-and-anti-diabetic-activity-of-methanol-and-water-extracts-of-nine-different-fruit-tree-leaves-collected-from-mediterranean-region-of-turkey" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/19405.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">486</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">768</span> In vitro α-Amylase and α-Glucosidase Inhibitory Activities of Bitter Melon (Momordica charantia) with Different Stage of Maturity</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=P.%20S.%20Percin">P. S. Percin</a>, <a href="https://publications.waset.org/abstracts/search?q=O.%20Inanli"> O. Inanli</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20Karakaya"> S. Karakaya</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Bitter melon (Momordica charantia) is a medicinal vegetable, which is used traditionally to remedy diabetes. Bitter melon contains several classes of primary and secondary metabolites. In traditional Turkish medicine bitter melon is used for wound healing and treatment of peptic ulcers. Nowadays, bitter melon is used for the treatment of diabetes and ulcerative colitis in many countries. The main constituents of bitter melon, which are responsible for the anti-diabetic effects, are triterpene, protein, steroid, alkaloid and phenolic compounds. In this study total phenolics, total carotenoids and β-carotene contents of mature and immature bitter melons were determined. In addition, in vitro α-amylase and α-glucosidase activities of mature and immature bitter melons were studied. Total phenolic contents of immature and mature bitter melon were 74 and 123 mg CE/g bitter melon respectively. Although total phenolics of mature bitter melon was higher than that of immature bitter melon, this difference was not found statistically significant (p > 0.05). Carotenoids, a diverse group of more than 600 naturally occurring red, orange and yellow pigments, play important roles in many physiological processes both in plants and humans. The total carotenoid content of mature bitter melon was 4.36 fold higher than the total carotenoid content of immature bitter melon. The compounds that have hypoglycaemic effect of bitter melon are steroidal saponins known as charantin, insulin-like peptides and alkaloids. α-Amylase is one of the main enzymes in human that is responsible for the breakdown of starch to more simple sugars. Therefore, the inhibitors of this enzyme can delay the carbohydrate digestion and reduce the rate of glucose absorption. The immature bitter melon extract showed α-amylase and α-glucosidase inhibitory activities in vitro. α-Amylase inhibitory activity was higher than that of α-glucosidase inhibitory activity when IC50 values were compared. In conclusion, the present results provide evidence that aqueous extract of bitter melon may have an inhibitory effect on carbohydrate breakdown enzymes. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bitter%20melon" title="bitter melon">bitter melon</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vitro%20antidiabetic%20activity" title=" in vitro antidiabetic activity"> in vitro antidiabetic activity</a>, <a href="https://publications.waset.org/abstracts/search?q=total%20carotenoids" title=" total carotenoids"> total carotenoids</a>, <a href="https://publications.waset.org/abstracts/search?q=total%20phenols" title=" total phenols"> total phenols</a> </p> <a href="https://publications.waset.org/abstracts/81770/in-vitro-a-amylase-and-a-glucosidase-inhibitory-activities-of-bitter-melon-momordica-charantia-with-different-stage-of-maturity" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/81770.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">241</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">767</span> Evaluation of the Inhibitory Activity of Natural Extracts From Spontaneous Plant on the Α-Amylase and Α–Glucosidase and Their Antioxidant Activities</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ihcen%20Khacheba">Ihcen Khacheba</a>, <a href="https://publications.waset.org/abstracts/search?q=Amar%20Djeridane"> Amar Djeridane</a>, <a href="https://publications.waset.org/abstracts/search?q=Abdelkarim%20%20Kamli"> Abdelkarim Kamli</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohamed%20Yousfi"> Mohamed Yousfi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Plant materials constitute an important source of natural bioactive molecules. Thus plants have been used from antiquity as sources of medicament against various diseases. These properties are usually attributed to secondary metabolites that are the subject of a lot of research in this field. This is particularly the case of phenolic compounds plants that are widely renowned in therapeutics as anti-inflammatories, enzyme inhibitors, and antioxidants, particularly flavonoïds. With the aim of acquiring a better knowledge of the secondary metabolism of the vegetable kingdom in the region of Laghouat and of the discovering of new natural therapeutics, 10 extracts from 5 Saharan plant species were submitted to chemical screening.The analysis of the preceding biological targets led to the evaluation of the biological activity of the extracts of the species Genista Corsica. The first step, consists in extracting and quantifying phenolic compounds. The second step has been devoted to stugying the effects of phenolic compounds on the kinetics catalyzed by two enzymes belonging to the class of hydrolase (the α-amylase and α-glucosidase) responsible for the digestion of sugars and finally we evaluate the antiantioxidant potential. The analysis results of phenolic extracts show clearly a low content of phenolic compounds in investigated plants. Average total phenolics ranged from 0.0017 to 11.35 mg equivalent gallic acid/g of the crude extract. Whereas the total flavonoids content lie between 0.0015 and 10.,96 mg/g equivalent of rutin. The results of the kinetic study of enzymatic reactions show that the extracts have inhibitory effects on both enzymes, with IC50 values ranging from 95.03 µg/ml to 1033.53 µg/ml for the α-amylase and 279.99 µg/ml to 1215.43 µg/ml for α-glucosidase whose greatest inhibition was found for the acetone extract of June (IC50 = 95.03 µg/ml). The results the antioxidant activity determined by ABTS, DPPH, and phosphomolybdenum tests clearly showed a good antioxidant capacity comparatively to antioxidants taken as reference the biological potential of these plants and could find their use in medicine to replace synthetic products. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=phenolic%20extracts" title="phenolic extracts">phenolic extracts</a>, <a href="https://publications.waset.org/abstracts/search?q=inhibition%20effect" title=" inhibition effect"> inhibition effect</a>, <a href="https://publications.waset.org/abstracts/search?q=%CE%B1-amylase" title=" α-amylase"> α-amylase</a>, <a href="https://publications.waset.org/abstracts/search?q=%CE%B1-glucosidase" title=" α-glucosidase"> α-glucosidase</a>, <a href="https://publications.waset.org/abstracts/search?q=antioxidant%20activity" title=" antioxidant activity"> antioxidant activity</a> </p> <a href="https://publications.waset.org/abstracts/13349/evaluation-of-the-inhibitory-activity-of-natural-extracts-from-spontaneous-plant-on-the-a-amylase-and-a-glucosidase-and-their-antioxidant-activities" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/13349.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">387</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">766</span> Box Counting Dimension of the Union L of Trinomial Curves When α ≥ 1</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Kaoutar%20Lamrini%20Uahabi">Kaoutar Lamrini Uahabi</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohamed%20Atounti"> Mohamed Atounti</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In the present work, we consider one category of curves denoted by L(p, k, r, n). These curves are continuous arcs which are trajectories of roots of the trinomial equation zn = αzk + (1 − α), where z is a complex number, n and k are two integers such that 1 ≤ k ≤ n − 1 and α is a real parameter greater than 1. Denoting by L the union of all trinomial curves L(p, k, r, n) and using the box counting dimension as fractal dimension, we will prove that the dimension of L is equal to 3/2. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=feasible%20angles" title="feasible angles">feasible angles</a>, <a href="https://publications.waset.org/abstracts/search?q=fractal%20dimension" title=" fractal dimension"> fractal dimension</a>, <a href="https://publications.waset.org/abstracts/search?q=Minkowski%20sausage" title=" Minkowski sausage"> Minkowski sausage</a>, <a href="https://publications.waset.org/abstracts/search?q=trinomial%20curves" title=" trinomial curves"> trinomial curves</a>, <a href="https://publications.waset.org/abstracts/search?q=trinomial%20equation" title=" trinomial equation"> trinomial equation</a> </p> <a href="https://publications.waset.org/abstracts/87207/box-counting-dimension-of-the-union-l-of-trinomial-curves-when-a-1" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/87207.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">189</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">765</span> Effect of Oxytocin on Cytosolic Calcium Concentration of Alpha and Beta Cells in Pancreas</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Rauza%20Sukma%20Rita">Rauza Sukma Rita</a>, <a href="https://publications.waset.org/abstracts/search?q=Katsuya%20Dezaki"> Katsuya Dezaki</a>, <a href="https://publications.waset.org/abstracts/search?q=Yuko%20Maejima"> Yuko Maejima</a>, <a href="https://publications.waset.org/abstracts/search?q=Toshihiko%20Yada"> Toshihiko Yada</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Oxytocin is a nine-amino acid peptide synthesized in the paraventricular nucleus (PVN) and supraoptic nucleus (SON) of the hypothalamus. Oxytocin promotes contraction of the uterus during birth and milk ejection during breast feeding. Although oxytocin receptors are found predominantly in the breasts and uterus of females, many tissues and organs express oxytocin receptors, including the pituitary, heart, kidney, thymus, vascular endothelium, adipocytes, osteoblasts, adrenal gland, pancreatic islets, and many cell lines. On the other hand, in pancreatic islets, oxytocin receptors are expressed in both α-cells and β-cells with stronger expression in α- cells. However, to our knowledge there are no reports yet about the effect of oxytocin on cytosolic calcium reaction on α and β-cell. This study aims to investigate the effect of oxytocin on α-cells and β-cells and its oscillation pattern. Islet of Langerhans from wild type mice were isolated by collagenase digestion. Isolated and dissociated single cells either α-cells or β-cells on coverslips were mounted in an open chamber and superfused in HKRB. Cytosolic concentration ([Ca2+]i) in single cells were measured by fura-2 microfluorimetry. After measurement of [Ca2+]i, α-cells were identified by subsequent immunocytochemical staining using an anti-glucagon antiserum. In β-cells, the [Ca2+]i increase in response to oxytocin was observed only under 8.3 mM glucose condition, whereas in α-cells, [Ca2+]i an increase induced by oxytocin was observed in both 2.8 mM and 8.3 mM glucose. The oscillation incidence was induced more frequently in β-cells compared to α-cells. In conclusion, the present study demonstrated that oxytocin directly interacts with both α-cells and β-cells and induces increase of [Ca2+]i and its specific patterns. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=%CE%B1-cells" title="α-cells">α-cells</a>, <a href="https://publications.waset.org/abstracts/search?q=%CE%B2-cells" title=" β-cells"> β-cells</a>, <a href="https://publications.waset.org/abstracts/search?q=cytosolic%20calcium%20concentration" title=" cytosolic calcium concentration"> cytosolic calcium concentration</a>, <a href="https://publications.waset.org/abstracts/search?q=oscillation" title=" oscillation"> oscillation</a>, <a href="https://publications.waset.org/abstracts/search?q=oxytocin" title=" oxytocin"> oxytocin</a> </p> <a href="https://publications.waset.org/abstracts/58454/effect-of-oxytocin-on-cytosolic-calcium-concentration-of-alpha-and-beta-cells-in-pancreas" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/58454.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">193</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">764</span> Titanium Alloys for Cryogenic Gas Bottle Applications: A Comparative Study</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Bhanu%20Pant">Bhanu Pant</a>, <a href="https://publications.waset.org/abstracts/search?q=Sanjay%20H.%20Upadhyay"> Sanjay H. Upadhyay</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Titanium alloys, owing to their high specific strength coupled with excellent resistance to corrosion in many severe environments, find extensive usage in the aerospace sector. Alpha and beta lean Titanium alloys have an additional characteristic of exhibiting high toughness with an NTS/ UTS ratio greater than one down to liquid oxygen and liquid helium temperatures. The cryogenic stage of high-performance rockets utilizes cryo-fluid submerged pressurizing tanks to improve volume to mass performance factor. A superior volume-to-mass ratio is achieved for LH2-submerged pressurizing tanks as compared to those submerged in LOX. Such high-efficiency tanks for LH2 submerged application necessitate the use of difficult to process alpha type Ti5Al2.5Sn-ELI alloy, which requires close control of process parameters to develop the tanks. In the present paper, a comparison of this alpha-type cryogenic Titanium alloy has been brought out with conventional alpha-beta Ti6Al4V-ELI alloy, which is usable up to LOX temperatures. Specific challenges faced during the development of these cryogenic pressurizing tanks for a launch vehicle based on the author's experience are included in the paper on the comparatively lesser-studied alpha Ti5Al2.5Sn-ELI alloy. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cryogenic%20tanks" title="cryogenic tanks">cryogenic tanks</a>, <a href="https://publications.waset.org/abstracts/search?q=titanium%20Alloys" title=" titanium Alloys"> titanium Alloys</a>, <a href="https://publications.waset.org/abstracts/search?q=NTS%2FUTS%20ratio" title=" NTS/UTS ratio"> NTS/UTS ratio</a>, <a href="https://publications.waset.org/abstracts/search?q=alpha%20and%20alpha-beta%20ELI%20alloys" title=" alpha and alpha-beta ELI alloys"> alpha and alpha-beta ELI alloys</a> </p> <a href="https://publications.waset.org/abstracts/184964/titanium-alloys-for-cryogenic-gas-bottle-applications-a-comparative-study" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/184964.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">62</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">763</span> Qualitative and Quantitative Screening of Biochemical Compositions for Six Selected Marine Macroalgae from Mediterranean Coast of Egypt </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Madelyn%20N.%20Moawad">Madelyn N. Moawad</a>, <a href="https://publications.waset.org/abstracts/search?q=Hermine%20R.%20Z.%20Tadros"> Hermine R. Z. Tadros</a>, <a href="https://publications.waset.org/abstracts/search?q=Mary%20G.%20Ghobrial"> Mary G. Ghobrial</a>, <a href="https://publications.waset.org/abstracts/search?q=Ahmad%20R.%20Bassiouny"> Ahmad R. Bassiouny</a>, <a href="https://publications.waset.org/abstracts/search?q=Kamal%20M.%20Kandeel"> Kamal M. Kandeel</a>, <a href="https://publications.waset.org/abstracts/search?q=Athar%20Ata"> Athar Ata</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Seaweeds are potential renewable resources in marine environment. They provide an excellent source of bioactive substances such as dietary fibers and various functional polysaccharides that could potentially be used as ingredients for both human and animal health applications. The observations suggested that these bioactive compounds have strong antioxidant properties, which have beneficial effects on human health. The present research aimed at finding new chemical products from local marine macroalgae for natural medicinal uses and consumption for their nutritional values. Macroalgae samples were collected manually mainly from the Mediterranean Sea at shallow subtidal zone of Abu Qir Bay, Alexandria, Egypt. The chemical compositions of lyophilized materials of six selected macroalgal species; Colpomenia sinuosa, Sargassum linifolium, Padina pavonia, Pterocladiella capillacea, Laurencia pinnatifidia, and Caulerpa racemosa, were investigated for proteins using bovine serum albumin, and carbohydrates were assayed by phenol-sulfuric acid reaction. The macroalgae lipid was extracted with chloroform, methanol and phosphate buffer. Vitamins were extracted using trichloroacetic acid. Chlorophylls and total carotenoids were determined spectrophotometrically and total phenols were extracted with methanol. In addition, lipid-soluble, and water-soluble antioxidant, and anti α-glucosidase activities were measured spectrophotometrically. The antioxidant activity of hexane extracts was investigated using phosphomolybdenum reagent. The anti-α-glucosidase effect measurement was initiated by mixing α-glucosidase solution with p-nitrophenyl α-D-glucopyranoside. The results showed that the ash contents varied from 11.2 to 35.4 % on dry weight basis for P. capillacea and Laurencia pinnatifidia, respectively. The protein contents ranged from 5.63 % in brown macroalgae C. sinuosa to 8.73 % in P. pavonia. A relative wide range in carbohydrate contents was observed (20.06–46.75 %) for the test algal species. The highest lipid percentage was found in green alga C. racemosa (5.91%) followed by brown algae P. pavonia (3.57%) and C. sinuosa (2.64%). The phenolic contents varied from 1.32 mg GAE/g for C. sinuosa to 4.00 mg GAE/g in P. pavonia. The lipid-soluble compounds exhibited higher antioxidant capacity (73.18-145.95 µM/g) than that of the water-soluble ones ranging from 24.83 µM/g in C. racemosa to 74.07 µM/g in S. linifolium. The most potent anti-α-glucosidase activity was observed for P. pavonia with IC50 of 17.12 μg/ml followed by S. linifolium (IC50 = 71.75 μg/ml), C. racemosa (IC50 = 84.73 μg/ml), P. capillacea (IC50 = 92.16 μg/ml), C. sinuosa (IC50 = 112.44 μg/ml), and L. pinnatifida (IC50 = 115.11 μg/ml). <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=%CE%B1-glucosidase" title="α-glucosidase">α-glucosidase</a>, <a href="https://publications.waset.org/abstracts/search?q=lyophilized" title=" lyophilized"> lyophilized</a>, <a href="https://publications.waset.org/abstracts/search?q=macroalgae" title=" macroalgae"> macroalgae</a>, <a href="https://publications.waset.org/abstracts/search?q=spectrophotometrically" title=" spectrophotometrically"> spectrophotometrically</a> </p> <a href="https://publications.waset.org/abstracts/76229/qualitative-and-quantitative-screening-of-biochemical-compositions-for-six-selected-marine-macroalgae-from-mediterranean-coast-of-egypt" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/76229.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">303</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">762</span> A New Alpha-Amylase Inhibitor Isolated from the Stem Bark of Anthocleista Djalonensis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Oseyemi%20O.%20Olubomehin">Oseyemi O. Olubomehin</a>, <a href="https://publications.waset.org/abstracts/search?q=Edith%20O.%20Ajaiyeoba"> Edith O. Ajaiyeoba</a>, <a href="https://publications.waset.org/abstracts/search?q=Kio%20A.%20Abo"> Kio A. Abo</a>, <a href="https://publications.waset.org/abstracts/search?q=Eleonora%20D.%20Goosen"> Eleonora D. Goosen </a> </p> <p class="card-text"><strong>Abstract:</strong></p> Diabetes is a major degenerative disease of global concern and it is the third most lethal disease of mankind, accounting for about 3.2 million deaths annually. Lowering postprandial hyperglycemia by inhibition of carbohydrate hydrolyzing enzyme such as alpha-amylase is one of the therapeutic approaches to treat Type 2 Diabetes. Alpha-amylase inhibitors from plants have been found to be effective in managing postprandial hyperglycemia. In continuation of our anti-diabetic activities of this plant, bioassay-guided fractionation and isolation using 0.1-1.0 mg/mL furnished djalonenol, a monoterpene diol with a significant 53.7% α-amylase inhibition (p<0.001) from the stem bark which was comparable to acarbose which gave a 54.9% inhibition. Spectral characterization using Infra-red, Gas Chromatogrphy-Mass spectrometry, 1D and 2D NMR of the isolated compound was done to elucidate the structure of the compound. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=alpha-amylase%20inhibitor" title="alpha-amylase inhibitor">alpha-amylase inhibitor</a>, <a href="https://publications.waset.org/abstracts/search?q=hyperglycemia" title=" hyperglycemia"> hyperglycemia</a>, <a href="https://publications.waset.org/abstracts/search?q=postprandial" title=" postprandial"> postprandial</a>, <a href="https://publications.waset.org/abstracts/search?q=diabetes" title=" diabetes"> diabetes</a> </p> <a href="https://publications.waset.org/abstracts/19227/a-new-alpha-amylase-inhibitor-isolated-from-the-stem-bark-of-anthocleista-djalonensis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/19227.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">458</span> </span> </div> </div> <ul class="pagination"> <li class="page-item disabled"><span class="page-link">&lsaquo;</span></li> <li class="page-item active"><span class="page-link">1</span></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=alpha%20glucosidase&page=2">2</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=alpha%20glucosidase&page=3">3</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=alpha%20glucosidase&page=4">4</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=alpha%20glucosidase&page=5">5</a></li> <li class="page-item"><a class="page-link" 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