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expires=' + expires.toGMTString() + '; domain=.' + this.getMasterDomain() + ';'; }, /** * get the master domain based on business rules */ getMasterDomain : function () { // default the master domain var masterDomain = 'thermofisher.com'; // check for alternate domain portion of URL var regexMatch = document.location.hostname.match(/\.(thermofisher)\.(cn)/i); if (regexMatch !== null) { masterDomain = regexMatch[1] + '.' + regexMatch[2]; } return masterDomain; }, /** * Check if init has been initiated once * @type {Boolean} */ initted: false, /** * @return {Array} method returns an array of a given object's key properties */ init: function () { this.page.country = this.getCookie("CK_ISO_CODE") || 'us'; this.page.language = this.getCookie('CK_LANG_CODE') || 'en'; this.page.siteName = 'thermo'; //hard coded this.initMerchandisingData(); this.initUserData(); this.displayDigitalData(); this.formSubmitEvent(); }, //specific function to set type of the page // need specific function to keep page type separate from other page attributes setPageType: function (type) { this.setPageAttribute('type', type); 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Upgrade your media while maintaining your results."); digitalData.setPageAttribute('businessUnit', "Cell Biology"); digitalData.setPageAttribute('searchDimensions', "SearchDimensions:Cell_Culture_Transfection"); digitalData.setPageAttribute('productDivision', "BID"); </script><script type="text/javascript" src="/etc/designs/komodo/clientlibs/cq-shared.min.f2c42a120b9408864fb0ab6ffa75a542.js"></script><div class="content wide-template well-wrapper"><div class="container container-breadcrumb"><div class="row"><div class="span12"><div class="breadcrumb"><ul itemscope="" itemtype="http://schema.org/BreadcrumbList" class="breadcrumb"><li itemprop="itemlistElement" itemscope="" itemtype="http://schema.org/ListItem"><a href="/sg/en/home.html" itemprop="item"><span itemprop="name">Home</span></a><span class="divider">&rsaquo;</span><meta itemprop="position" content="1"></li><li itemprop="itemlistElement" itemscope="" itemtype="http://schema.org/ListItem"><a href="/sg/en/home/life-science.html" itemprop="item"><span itemprop="name">Life Sciences</span></a><span class="divider">&rsaquo;</span><meta itemprop="position" content="2"></li><li itemprop="itemlistElement" itemscope="" itemtype="http://schema.org/ListItem"><a href="/sg/en/home/life-science/cell-culture.html" itemprop="item"><span itemprop="name">Cell Culture & Transfection</span></a><span class="divider">&rsaquo;</span><meta itemprop="position" content="3"></li><li itemprop="itemlistElement" itemscope="" itemtype="http://schema.org/ListItem"><a href="/sg/en/home/life-science/cell-culture/mammalian-cell-culture.html" itemprop="item"><span itemprop="name">Mammalian Cell Culture</span></a><span class="divider">&rsaquo;</span><meta itemprop="position" content="4"></li><li itemprop="itemlistElement" itemscope="" itemtype="http://schema.org/ListItem"><a href="/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture.html" itemprop="item"><span itemprop="name">Cancer Cell Culture</span></a><span class="divider">&rsaquo;</span><meta itemprop="position" content="5"></li><li itemprop="itemlistElement" itemscope="" itemtype="http://schema.org/ListItem"><a href="/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture.html" itemprop="item"><span itemprop="name">Tumoroid Culture</span></a><span class="divider">&rsaquo;</span><meta itemprop="position" content="6"></li><li class="active" itemprop="itemlistElement" itemscope="" itemtype="http://schema.org/ListItem"><span itemprop="name">OncoPro Tumoroid Culture Medium Kit</span><meta itemprop="position" content="7"></li></ul></div></div></div></div><div class="container"><div class="bannerParsys iparsys parsys"><div class="section"><div class="new"></div></div><div class="iparys_inherited"><div class="bannerParsys iparsys parsys"><div class="thinbanner thinbanner-info section"><span id="thinbanner_7b474744-207f-419a-9353-206a5b886eecbannerParsysthinbanner_738c"></span><script>$CQ('#thinbanner_7b474744-207f-419a-9353-206a5b886eecbannerParsysthinbanner_738c').parent().hide();</script></div></div></div></div><div class="headerParsys parsys"></div></div><div class="container well-wrapper"><div class="row"><div class="span12"><div class="wellPageHeading"><div class="well-cmp"><div class="well well-white well-mini"><div class="well-container" style="height:autopx"><link rel="preload" href="/content/dam/LifeTech/global/life-sciences/bid/cell-biology/2023/05/tumoroid-product-banner.jpg"/><img src="/content/dam/LifeTech/global/life-sciences/bid/cell-biology/2023/05/tumoroid-product-banner.jpg" alt="" height="250px" width="1600px" class="well-background-image"/><div class="well-content"><div class="parsys"><div class="text parbase section"><div class="table-component-wrapper"><table id="table-2106065690"><tbody><tr><td style="vertical-align: middle; middle; height: 250px;"><h1 style="color: rgb(255, 255, 255); text-shadow: rgba(0, 0, 0, 0.8) 2px 2px 5px, rgb(0, 0, 0) 0px 0px 2px;">OncoPro Tumoroid Culture Medium Kit</h1><h3 style="color: rgb(255, 255, 255); text-shadow: rgba(0, 0, 0, 0.8) 2px 2px 5px, rgb(0, 0, 0) 0px 0px 2px;">Access simpler tumoroid culture with OncoPro Tumoroid Culture Medium</h3><br><p><a class="btn btn-featured" href="/sg/en/home/global/forms/oncopro-medium-info.html">Request discount</a></p></td></tr></tbody></table></div></div></div></div></div></div></div></div></div></div></div><div class="container page-header-hidden"><div class="row"><div class="span12"><div class="pageheading"></div></div></div></div><div class="container-wrap template-2-column"><div class="container"><div class="row"><script> var showHideText = { 'seeRelatedText':'See Navigation', 'hideRelatedText':'Hide Navigation' }; </script><div class="span3 sidebar" role="complementary"><div id="link-leftnav-toggle-container"><a class='link-leftnav-toggle' href='#'>See Navigation</a></div><div class="container-leftnav"><div class="railInheritParsys iparsys parsys"><div class="section"><div class="new"></div></div><div class="iparys_inherited"><div class="railInheritParsys iparsys parsys"></div></div></div><div class="railParsys parsys"></div></div></div><div class="span9 main" role="main"><div class="MainParsys parsys"><div class="well-cmp section"><div class="well "><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="text parbase section"><h3>Cancer organoid drug screening webinar</h3><p>Listen to Dr. Christophe Deben of Orbits Oncology share his experience with OncoPro medium in the recent webinar, "Advancing cancer organoid drug screening with novel media systems."</p><p><a class="cta-link" href="https://www.labroots.com/ms/webinar/advancing-cancer-organoid-drug-screening-novel-media-systems?campaign=0524oncoprowebinar" target="_blank">Watch it on demand now</a></p></div></div></div></div></div></div><div class="text parbase section"><p>Gibco OncoPro Tumoroid Culture Medium streamlines the culture of patient-derived cancer organoids (tumoroids) to help make physiologically relevant cancer models more accessible. This adaptable culture system is readily integrated into your workflow so you can upgrade your media and maintain your results. Overall, OncoPro medium offers several key benefits to cancer researchers:</p><div class="table-component-wrapper"><table cellpadding="5" cellspacing="0" border="0" id="table-34026623"><tbody><tr><td><a href="?open=optimized#optimized"><img src="/content/dam/LifeTech/global/life-sciences/bid/cell-biology/2023/05/cancer-icon.png" title="Cancer icon" alt="Cancer icon" width="50"></a></td><td><p><a href="?open=optimized#optimized"><b>Optimized</b></a>—OncoPro medium was developed to support expansion of tumoroids without overgrowth of non-malignant cells. As such, this culture system helps ensure preservation of donor-specific characteristics, including mutational profiles and gene expression patterns, in long-term culture.</p></td></tr><tr><td><a href="?open=streamlined#streamlined"><img src="/content/dam/LifeTech/global/life-sciences/bid/cell-biology/2023/05/gibco-bottle-icon.png" title=" Gibco bottle icon " alt=" Gibco bottle icon " width="50"></a></td><td><p><a href="?open=streamlined#streamlined"><b>Streamlined</b></a>—OncoPro medium is a modular media system, consisting of four components that can be supplemented with indication-specific growth factors as needed. Its suspension culture capability introduces a simplified tumoroid culture workflow, helping save time and resources.</p></td></tr><tr><td><a href="?open=adaptable#adaptable"><img src="/content/dam/LifeTech/global/life-sciences/bid/cell-biology/2023/05/split-arrow-icon.png" title="Split arrow icon" alt="Split arrow icon" width="50"></a></td><td><p><a href="?open=adaptable#adaptable"><b>Adaptable</b></a>—OncoPro medium is compatible with a suspension or embedded tumoroid workflow, supports scale-up and automation, and sustains tumoroids across multiple cancer indications. OncoPro medium is also compatible with traditional applications and assays.</p></td></tr></tbody></table></div></div><div class="text parbase section"><p><a href="#order"><span class="btn btn-primary">Order now</span></a>     <a href="/sg/en/home/global/forms/oncopro-medium-info.html"><span class="btn btn-primary">Contact us</span></a></p></div><div class="well-cmp section"><div class="well "><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="text parbase section"><h3>On this page:</h3></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="text parbase section"><ul><li><a href="#stories">Customer stories</a></li><li><a href="#benefits">Benefits and performance data</a></li><li><a href="#literature">Technical literature</a></li></ul></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="text parbase section"><ul><li><a href="#order">Order reagents</a></li><li><a href="#resources">Additional resources</a></li><li><a href="#faq">FAQs</a></li></ul></div></div></div></div><div style="clear:both"></div></div></div></div></div></div><div class="heading section"><h2 id="stories"><br>Customer stories </h2></div><div class="textimage parbase textimage-left section"><div class="image" id="cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystextimage_a75a"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_a75a/image.img.full.high.jpg/1732297880876.jpg' media="(min-width: 1px)"><img class='textimage-auto' srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_a75a/image.img.full.high.jpg/1732297880876.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_a75a/image.img.320.high.jpg/1732297880876.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_a75a/image.img.480.high.jpg/1732297880876.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_a75a/image.img.320.medium.jpg/1732297880876.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_a75a/image.img.620.high.jpg/1732297880876.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_a75a/image.img.320.low.jpg/1732297880876.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_a75a/image.img.480.medium.jpg/1732297880876.jpg" alt='Erik Goka' title='Erik Goka'></picture></div><style> #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystextimage_a75a { max-width: 100%; } #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystextimage_a75a img { width: 100%; max-width: 200px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p>"The Gibco OncoPro Tumoroid Culture Medium system allows us to seamlessly integrate tumor organoids into our research and drug discovery processes. With the tumoroid media system we can propagate all our organoid models in the same culture medium system irrespective of the tissue of origin. This media system has been instrumental in allowing us to bring tumor organoids into our lab for everyday use."</p><h4>Erik Goka, PhD<br> Vice President of Biology<br> Revere Pharmaceuticals</h4></div></div><div class="clearfix"></div></div><div class="textimage parbase textimage-left section"><div class="image" id="cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystextimage_1de8"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_1de8/image.img.320.high.jpg/1732297880901.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_1de8/image.img.480.high.jpg/1732297880901.jpg' media="(min-width: 490px)"><img class='textimage-auto' srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_1de8/image.img.full.high.jpg/1732297880901.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_1de8/image.img.320.high.jpg/1732297880901.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_1de8/image.img.480.high.jpg/1732297880901.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_1de8/image.img.320.medium.jpg/1732297880901.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_1de8/image.img.620.high.jpg/1732297880901.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_1de8/image.img.320.low.jpg/1732297880901.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_1de8/image.img.480.medium.jpg/1732297880901.jpg" alt='Shawn Fahl' title='Shawn Fahl'></picture></div><style> #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystextimage_1de8 { max-width: 100%; } #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystextimage_1de8 img { width: 100%; max-width: 577px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p>"The OncoPro media has greatly accelerated our development of long-term primary tumor culture systems. The media is easy to make, with a minimal amount of separate components compared to other systems, and the protocols are straightforward, allowing the transition from culture initiation to viable long-term cultures to be seamless and quick."</p><h4>Shawn Fahl, PhD<br>V.P. Lab Operations, Cell Services & R&D,<br>BiospecimensDiscovery Life Sciences</h4></div></div><div class="clearfix"></div></div><div class="heading section"><h2 id="benefits"><br>Benefits and performance data of OncoPro medium </h2></div><div class="tabworkflowcontainer section"><div class="tabbable plain-tab"><ul class="nav nav-tabs" id="myTab"><li class="active"><a data-toggle="tab" href="#container-ae9f1139481541">Optimized performance</a></li><li><a data-toggle="tab" href="#container-ae9f1139481542">Streamlined adoption </a></li><li><a data-toggle="tab" href="#container-ae9f1139481543">Adaptable application</a></li></ul><div class="tab-content" id="myTabContent"><div id="container-ae9f1139481541" class="tab-pane active"><div class="container-ae9f1139481541 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h3> Discover how OncoPro medium was optimized for tumoroid culture </h3></div><div class="text parbase section"><p>OncoPro Tumoroid Culture Medium is a novel media system that was specifically developed for the expansion of patient-derived tumoroids. Over 40 conditions were tested during the development of OncoPro to identify the combination of basal media, supplements, and growth factors that would best support long-term maintenance of donor-specific characteristics, including mutational profiles and gene expression patterns. <b></b></p></div><div class="textimage parbase textimage-top section"><div class="image" id="cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481541tabtextimage_d12c"><a class="image-enlarge-link" data-lightbox="/content/lifetech/ipac/en-sg/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_d12c" href="/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/_jcr_content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_d12c/backgroundimg.img.jpg/1732297881020.jpg"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_d12c/image.img.320.high.jpg/1732297880999.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_d12c/image.img.480.high.jpg/1732297880999.jpg' media="(min-width: 490px)"><img class='textimage-auto' srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_d12c/image.img.full.high.jpg/1732297880999.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_d12c/image.img.320.high.jpg/1732297880999.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_d12c/image.img.480.high.jpg/1732297880999.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_d12c/image.img.320.medium.jpg/1732297880999.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_d12c/image.img.620.high.jpg/1732297880999.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_d12c/image.img.320.low.jpg/1732297880999.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_d12c/image.img.480.medium.jpg/1732297880999.jpg" alt='Compilation of graphs illustrating tumoroid response' title='Tumoroid response to 25 unique media conditions'></picture></div><style> #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481541tabtextimage_d12c { max-width: 100%; } #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481541tabtextimage_d12c img { width: 100%; max-width: 579px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b>Figure 1. Design of experiment studies were used to fully optimize the OncoPro medium formulation for tumoroid growth</b>. Combinations of medium components were tested in designed experiments to elucidate main effects and interactions of additives on tumoroid growth. In this example, two tumoroid lines were tested using 25 media recipes, with 1-2 replicate wells per condition. (A) Heat map of number of viable cells detected per media condition as measured by PrestoBlue HS assay. (B) Images of tumoroids in medium optimization experiments. Different conditions were tested on dissociated cells seeded from an identical initial cell pool, and conditions advantageous to tumoroid growth and retention of genetic and phenotypic characteristics were identified. (C) Design of experiment output for tumoroid line growth response to media optimization experimental conditions. Figure adapted from JMP 16 software output.</small></p></div></div><div class="clearfix"></div></div><div class="textimage parbase textimage-top section"><div class="image" id="cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481541tabtextimage_a931"><a class="image-enlarge-link" data-lightbox="/content/lifetech/ipac/en-sg/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_a931" href="/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/_jcr_content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_a931/image.img.jpg/1732297881046.jpg"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_a931/image.img.320.high.jpg/1732297881028.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_a931/image.img.480.high.jpg/1732297881028.jpg' media="(min-width: 490px)"><img class='textimage-auto' srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_a931/image.img.full.high.jpg/1732297881028.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_a931/image.img.320.high.jpg/1732297881028.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_a931/image.img.480.high.jpg/1732297881028.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_a931/image.img.320.medium.jpg/1732297881028.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_a931/image.img.620.high.jpg/1732297881028.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_a931/image.img.320.low.jpg/1732297881028.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_a931/image.img.480.medium.jpg/1732297881028.jpg" alt='Bar chart of tumor marker expression and microscopic view of stained tumor cells' title='OncoPro medium selectively expands cancer cells'></picture></div><style> #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481541tabtextimage_a931 { max-width: 100%; } #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481541tabtextimage_a931 img { width: 100%; max-width: 579px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b>Figure 2. OncoPro medium selectively expands cancer cells. </b>(A) Marker expression (flow cytometry) of cells freshly isolated from a colorectal tumor resection (never in culture) and tumoroid cells after line establishment (i.e., 7 passages in OncoPro medium). The panel included markers for epithelial-derived cells (EpCAM), colorectal cancer cells (CEACAM), immune cells (CD45), endothelial cells (CD31), and mesenchymal cells (vimentin). Data reveal preferential outgrowth of epithelial colorectal cancer cells over immune or endothelial cells. (B) Representative immunofluorescence image of EpCAM and actin (phalloidin) expression demonstrate that tumoroids are highly enriched for EpCAM-positive tumor cells.</small></p></div></div><div class="clearfix"></div></div><div class="textimage parbase textimage-left section"><div class="image" id="cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481541tabtextimage_7d5f"><a class="image-enlarge-link" data-lightbox="/content/lifetech/ipac/en-sg/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_7d5f" href="/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/_jcr_content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_7d5f/image.img.jpg/1732297881080.jpg"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_7d5f/image.img.320.high.jpg/1732297881061.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_7d5f/image.img.480.high.jpg/1732297881061.jpg' media="(min-width: 490px)"><img class='textimage-auto' srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_7d5f/image.img.full.high.jpg/1732297881061.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_7d5f/image.img.320.high.jpg/1732297881061.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_7d5f/image.img.480.high.jpg/1732297881061.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_7d5f/image.img.320.medium.jpg/1732297881061.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_7d5f/image.img.620.high.jpg/1732297881061.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_7d5f/image.img.320.low.jpg/1732297881061.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_7d5f/image.img.480.medium.jpg/1732297881061.jpg" alt='Correlation graph representing maintenance of mutational profiles' title='Long-term maintenance of mutational profiles'></picture></div><style> #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481541tabtextimage_7d5f { max-width: 100%; } #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481541tabtextimage_7d5f img { width: 100%; max-width: 579px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b>Figure 3. OncoPro medium helps maintain mutational profiles of tumoroid lines over long-term culture. </b>Correlation of germline and somatic single nucleotide variations (SNVs) between early and later-passage tumoroid cultures. Each dot represents the variant allelic frequency (VAF) for one genetic locus covered by the Oncomine Comprehensive Assay v3. Figure adapted from JMP 16 software output.</small></p></div></div><div class="clearfix"></div></div></div><div class="end"></div></div></div><div id="container-ae9f1139481542" class="tab-pane"><div class="container-ae9f1139481542 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h3> Understand how OncoPro medium streamlines media formulation and tumoroid culture </h3></div><div class="text parbase section"><p>OncoPro medium affords researchers workflow versatility by letting users decide if they want to utilize established embedded culture methods or transition to suspension methods to help save both time and resources.</p></div><div class="textimage parbase textimage-top section"><div class="image" id="cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481542tabtextimage_af58"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_af58/image.img.320.high.jpg/1732297881127.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_af58/image.img.480.high.jpg/1732297881127.jpg' media="(min-width: 490px)"><img class='textimage-auto' srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_af58/image.img.full.high.jpg/1732297881127.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_af58/image.img.320.high.jpg/1732297881127.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_af58/image.img.480.high.jpg/1732297881127.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_af58/image.img.320.medium.jpg/1732297881127.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_af58/image.img.620.high.jpg/1732297881127.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_af58/image.img.320.low.jpg/1732297881127.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_af58/image.img.480.medium.jpg/1732297881127.jpg" alt='Scientific diagrams showing tumoroid culture methods' title='OncoPro medium suspension culture simplifies tumoroid passaging'></picture></div><style> #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481542tabtextimage_af58 { max-width: 100%; } #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481542tabtextimage_af58 img { width: 100%; max-width: 579px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b>Figure 4. The OncoPro medium suspension culture method greatly simplifies tumoroid passaging, reducing hands-on time from 140 minutes for embedded culture to 60 minutes for suspension culture.</b> (A) Schematic of culture method steps for passaging tumoroids in embedded culture. Boxes highlight the pain points of disrupting extracellular matrix (ECM) domes, losing cells trapped in ECM during washing, and pipetting and waiting for ECM domes to solidify, respectively. (B) Schematic of culture method steps for passaging tumoroids in suspension culture, which addresses these pain points due to reduced ECM requirement.<br> Figure created with BioRender.com.</small></p></div></div><div class="clearfix"></div></div><div class="textimage parbase textimage-top section"><div class="image" id="cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481542tabtextimage_95dd"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_95dd/image.img.320.high.jpg/1732297881156.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_95dd/image.img.480.high.jpg/1732297881156.jpg' media="(min-width: 490px)"><img class='textimage-auto' srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_95dd/image.img.full.high.jpg/1732297881156.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_95dd/image.img.320.high.jpg/1732297881156.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_95dd/image.img.480.high.jpg/1732297881156.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_95dd/image.img.320.medium.jpg/1732297881156.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_95dd/image.img.620.high.jpg/1732297881156.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_95dd/image.img.320.low.jpg/1732297881156.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_95dd/image.img.480.medium.jpg/1732297881156.jpg" alt='Table comparing suspension vs embedded culture' title='OncoPro suspension cultures is ready to scale'></picture></div><style> #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481542tabtextimage_95dd { max-width: 100%; } #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481542tabtextimage_95dd img { width: 100%; max-width: 579px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b>Figure 5. Tumoroid cultures are readily scalable with the OncoPro medium suspension culture method.</b> The number and format of culture vessels required to obtain increasing scale of tumoroid cultures are more user- and resource-friendly in suspension culture when compared to traditional embedded culture methods. (A) Suspension methods allow for ready expansion of 100 million cells within one passage, whereas the number of basement membrane extract (BME) domes required in embedded culture would be prohibitive for many users. (B) Average amount of BME required to harvest 100 million cells for 9 tumoroid lines using different culture methods. On average, suspension culture required less BME than embedded culture (mean ± SEM, not significant).</small></p></div></div><div class="clearfix"></div></div><div class="textimage parbase textimage-left section"><div class="image" id="cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481542tabtextimage_2f3a"><a class="image-enlarge-link" data-lightbox="/content/lifetech/ipac/en-sg/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_2f3a" href="/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/_jcr_content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_2f3a/image.img.jpg/1732297881202.jpg"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_2f3a/image.img.full.high.jpg/1732297881186.jpg' media="(min-width: 1px)"><img class='textimage-auto' srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_2f3a/image.img.full.high.jpg/1732297881186.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_2f3a/image.img.320.high.jpg/1732297881186.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_2f3a/image.img.480.high.jpg/1732297881186.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_2f3a/image.img.320.medium.jpg/1732297881186.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_2f3a/image.img.620.high.jpg/1732297881186.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_2f3a/image.img.320.low.jpg/1732297881186.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_2f3a/image.img.480.medium.jpg/1732297881186.jpg" alt='Correlation graph representing patient-specific gene expression' title='Patient-specific gene expression profiles'></picture></div><style> #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481542tabtextimage_2f3a { max-width: 100%; } #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481542tabtextimage_2f3a img { width: 100%; max-width: 426px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b>Figure 6. Patient-specific gene expression profiles are highly correlated between samples cultured using the streamlined suspension method and those cultured using traditional embedded methods. </b>Ward hierarchical clustering and Spearman’s ρ correlations of gene expression for a panel of 1423 cancer-related genes across a variety of tumoroid lines representing four different cancer indications. Gene expression assayed by Ion AmpliSeq Transcriptome Human Gene Expression Panel, Chef-Ready Kit. Figures adapted from JMP 16 software output. With the exception of colorectal tumoroid line Z, established tumoroid models provided by NCI PDMR. We thank the PDMR and their contributing institutions for their contributions to this work.</small></p></div></div><div class="clearfix"></div></div></div><div class="end"></div></div></div><div id="container-ae9f1139481543" class="tab-pane"><div class="container-ae9f1139481543 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h3> Learn how OncoPro medium functions as an adaptable system </h3></div><div class="text parbase section"><p>OncoPro Tumoroid Culture Medium Kit is a flexible system designed to address complex research needs. OncoPro medium accommodates multiple cancer indications using a single media kit and the scalable and automation-friendly suspension workflow facilitates smooth transitions to various downstream assays. The absence of Wnt, R-spondin, Noggin, and small molecule inhibitors make OncoPro well-suited for advanced biological studies and screening applications.</p></div><div class="textimage parbase textimage-left section"><div class="image" id="cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481543tabtextimage_905e"><a class="image-enlarge-link" data-lightbox="/content/lifetech/ipac/en-sg/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_905e" href="/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/_jcr_content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_905e/backgroundimg.img.jpg/1732297881271.jpg"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_905e/image.img.full.high.jpg/1732297881249.jpg' media="(min-width: 1px)"><img class='textimage-auto' srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_905e/image.img.full.high.jpg/1732297881249.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_905e/image.img.320.high.jpg/1732297881249.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_905e/image.img.480.high.jpg/1732297881249.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_905e/image.img.320.medium.jpg/1732297881249.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_905e/image.img.620.high.jpg/1732297881249.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_905e/image.img.320.low.jpg/1732297881249.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_905e/image.img.480.medium.jpg/1732297881249.jpg" alt='Images of tumoroids' title='Tumoroid morphology comparison'></picture></div><style> #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481543tabtextimage_905e { max-width: 100%; } #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481543tabtextimage_905e img { width: 100%; max-width: 426px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><small><b>Figure 7. OncoPro medium is compatible with four different cancer indications, for both suspension and embedded culture.</b> Morphology of NCI PDMR tumoroid lines expanded in NCI PDMR homebrew media (embedded culture), or in OncoPro medium embedded or suspension culture. Scale bar = 400 µm. Established tumoroid models provided by NCI PDMR. We thank the PDMR and their contributing institutions for their contributions to this work.</small></div></div><div class="clearfix"></div></div><div class="textimage parbase textimage-left section"><div class="image" id="cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481543tabtextimage_ddc6"><a class="image-enlarge-link" data-lightbox="/content/lifetech/ipac/en-sg/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_ddc6" href="/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/_jcr_content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_ddc6/backgroundimg.img.jpg/1732297881300.jpg"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_ddc6/image.img.full.high.jpg/1732297881279.jpg' media="(min-width: 1px)"><img class='textimage-auto' srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_ddc6/image.img.full.high.jpg/1732297881279.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_ddc6/image.img.320.high.jpg/1732297881279.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_ddc6/image.img.480.high.jpg/1732297881279.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_ddc6/image.img.320.medium.jpg/1732297881279.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_ddc6/image.img.620.high.jpg/1732297881279.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_ddc6/image.img.320.low.jpg/1732297881279.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_ddc6/image.img.480.medium.jpg/1732297881279.jpg" alt='Graphs of tumoroid population doubling vs days' title='Tumoroid doubling time evaluation'></picture></div><style> #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481543tabtextimage_ddc6 { max-width: 100%; } #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481543tabtextimage_ddc6 img { width: 100%; max-width: 426px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b>Figure 8. OncoPro medium is compatible with four different cancer indications, for both suspension and embedded culture. </b>Cumulative population doublings of cells expanded in OncoPro medium suspension culture (purple), OncoPro medium embedded culture (red), or alternative media system (media system in which the line was derived prior to procurement) embedded culture (green). In general, tumoroids expanded in each culture condition with comparable growth rates. With the exception of colorectal tumoroid line Z, established tumoroid models provided by NCI PDMR. We thank the PDMR and their contributing institutions for their contributions to this work.</small></p></div></div><div class="clearfix"></div></div><div class="textimage parbase textimage-left section"><div class="image" id="cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481543tabtextimage_69d7"><a class="image-enlarge-link" data-lightbox="/content/lifetech/ipac/en-sg/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_69d7" href="/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/_jcr_content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_69d7/image.img.jpg/1732297881324.jpg"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_69d7/image.img.full.high.jpg/1732297881308.jpg' media="(min-width: 1px)"><img class='textimage-auto' srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_69d7/image.img.full.high.jpg/1732297881308.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_69d7/image.img.320.high.jpg/1732297881308.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_69d7/image.img.480.high.jpg/1732297881308.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_69d7/image.img.320.medium.jpg/1732297881308.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_69d7/image.img.620.high.jpg/1732297881308.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_69d7/image.img.320.low.jpg/1732297881308.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_69d7/image.img.480.medium.jpg/1732297881308.jpg" alt='Correlation graph representing patient-specific gene expression' title='Patient-specific gene expression profiles'></picture></div><style> #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481543tabtextimage_69d7 { max-width: 100%; } #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481543tabtextimage_69d7 img { width: 100%; max-width: 426px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b>Figure 9. OncoPro medium helps maintain patient-specific gene expression profiles from lines derived in alternative media and embedded culture. </b>Principal component analysis (PCA) plot comparing gene expression levels across tumoroid lines and culture conditions. Tumoroid lines were expanded in parallel in multiple media and culture conditions for 2-7 passages post-thaw prior to quantification of expression levels across over >20,000 human RefSeq genes using the Ion AmpliSeq Transcriptome Human Gene Expression Kit. Tumoroid lines tested were 782815-120-R-V1-organoid (Colorectal A), 919269-233-R3-V2-organoid (Colorectal B), 349418-098-R-V2-organoid (Lung), 549293-155-R-V1-organoid (Head and Neck), and 699152-130-R-V1-organoid (Pancreas). With the exception of colorectal tumoroid line Z, established tumoroid models were provided by NCI PDMR. We thank the PDMR and their contributing institutions for their contributions to this work. Figure adapted from TAC 4.0 software output.</small></p></div></div><div class="clearfix"></div></div></div><div class="end"></div></div></div></div></div><div class="workflow-accordion accordion" id="accordion-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154"><div class="accordion-group"><div class="accordion-heading active"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154" href="#collapse1"><span class="caret"></span><strong> Optimized performance </strong></a></div><div id="collapse1" class="accordion-body collapse in "><div class="accordion-inner container-ae9f1139481541"><div class="container-ae9f1139481541 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h3> Discover how OncoPro medium was optimized for tumoroid culture </h3></div><div class="text parbase section"><p>OncoPro Tumoroid Culture Medium is a novel media system that was specifically developed for the expansion of patient-derived tumoroids. Over 40 conditions were tested during the development of OncoPro to identify the combination of basal media, supplements, and growth factors that would best support long-term maintenance of donor-specific characteristics, including mutational profiles and gene expression patterns. <b></b></p></div><div class="textimage parbase textimage-top section"><div class="image" id="cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481541tabtextimage_d12c"><a class="image-enlarge-link" data-lightbox="/content/lifetech/ipac/en-sg/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_d12c" href="/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/_jcr_content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_d12c/backgroundimg.img.jpg/1732297881020.jpg"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_d12c/image.img.320.high.jpg/1732297880999.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_d12c/image.img.480.high.jpg/1732297880999.jpg' media="(min-width: 490px)"><img class='textimage-auto' srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_d12c/image.img.full.high.jpg/1732297880999.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_d12c/image.img.320.high.jpg/1732297880999.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_d12c/image.img.480.high.jpg/1732297880999.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_d12c/image.img.320.medium.jpg/1732297880999.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_d12c/image.img.620.high.jpg/1732297880999.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_d12c/image.img.320.low.jpg/1732297880999.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_d12c/image.img.480.medium.jpg/1732297880999.jpg" alt='Compilation of graphs illustrating tumoroid response' title='Tumoroid response to 25 unique media conditions'></picture></div><style> #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481541tabtextimage_d12c { max-width: 100%; } #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481541tabtextimage_d12c img { width: 100%; max-width: 579px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b>Figure 1. Design of experiment studies were used to fully optimize the OncoPro medium formulation for tumoroid growth</b>. Combinations of medium components were tested in designed experiments to elucidate main effects and interactions of additives on tumoroid growth. In this example, two tumoroid lines were tested using 25 media recipes, with 1-2 replicate wells per condition. (A) Heat map of number of viable cells detected per media condition as measured by PrestoBlue HS assay. (B) Images of tumoroids in medium optimization experiments. Different conditions were tested on dissociated cells seeded from an identical initial cell pool, and conditions advantageous to tumoroid growth and retention of genetic and phenotypic characteristics were identified. (C) Design of experiment output for tumoroid line growth response to media optimization experimental conditions. Figure adapted from JMP 16 software output.</small></p></div></div><div class="clearfix"></div></div><div class="textimage parbase textimage-top section"><div class="image" id="cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481541tabtextimage_a931"><a class="image-enlarge-link" data-lightbox="/content/lifetech/ipac/en-sg/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_a931" href="/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/_jcr_content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_a931/image.img.jpg/1732297881046.jpg"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_a931/image.img.320.high.jpg/1732297881028.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_a931/image.img.480.high.jpg/1732297881028.jpg' media="(min-width: 490px)"><img class='textimage-auto' srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_a931/image.img.full.high.jpg/1732297881028.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_a931/image.img.320.high.jpg/1732297881028.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_a931/image.img.480.high.jpg/1732297881028.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_a931/image.img.320.medium.jpg/1732297881028.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_a931/image.img.620.high.jpg/1732297881028.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_a931/image.img.320.low.jpg/1732297881028.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_a931/image.img.480.medium.jpg/1732297881028.jpg" alt='Bar chart of tumor marker expression and microscopic view of stained tumor cells' title='OncoPro medium selectively expands cancer cells'></picture></div><style> #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481541tabtextimage_a931 { max-width: 100%; } #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481541tabtextimage_a931 img { width: 100%; max-width: 579px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b>Figure 2. OncoPro medium selectively expands cancer cells. </b>(A) Marker expression (flow cytometry) of cells freshly isolated from a colorectal tumor resection (never in culture) and tumoroid cells after line establishment (i.e., 7 passages in OncoPro medium). The panel included markers for epithelial-derived cells (EpCAM), colorectal cancer cells (CEACAM), immune cells (CD45), endothelial cells (CD31), and mesenchymal cells (vimentin). Data reveal preferential outgrowth of epithelial colorectal cancer cells over immune or endothelial cells. (B) Representative immunofluorescence image of EpCAM and actin (phalloidin) expression demonstrate that tumoroids are highly enriched for EpCAM-positive tumor cells.</small></p></div></div><div class="clearfix"></div></div><div class="textimage parbase textimage-left section"><div class="image" id="cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481541tabtextimage_7d5f"><a class="image-enlarge-link" data-lightbox="/content/lifetech/ipac/en-sg/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_7d5f" href="/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/_jcr_content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_7d5f/image.img.jpg/1732297881080.jpg"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_7d5f/image.img.320.high.jpg/1732297881061.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_7d5f/image.img.480.high.jpg/1732297881061.jpg' media="(min-width: 490px)"><img class='textimage-auto' srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_7d5f/image.img.full.high.jpg/1732297881061.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_7d5f/image.img.320.high.jpg/1732297881061.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_7d5f/image.img.480.high.jpg/1732297881061.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_7d5f/image.img.320.medium.jpg/1732297881061.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_7d5f/image.img.620.high.jpg/1732297881061.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_7d5f/image.img.320.low.jpg/1732297881061.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481541/tab/textimage_7d5f/image.img.480.medium.jpg/1732297881061.jpg" alt='Correlation graph representing maintenance of mutational profiles' title='Long-term maintenance of mutational profiles'></picture></div><style> #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481541tabtextimage_7d5f { max-width: 100%; } #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481541tabtextimage_7d5f img { width: 100%; max-width: 579px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b>Figure 3. OncoPro medium helps maintain mutational profiles of tumoroid lines over long-term culture. </b>Correlation of germline and somatic single nucleotide variations (SNVs) between early and later-passage tumoroid cultures. Each dot represents the variant allelic frequency (VAF) for one genetic locus covered by the Oncomine Comprehensive Assay v3. Figure adapted from JMP 16 software output.</small></p></div></div><div class="clearfix"></div></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154" href="#collapse2"><span class="caret"></span><strong> Streamlined adoption </strong></a></div><div id="collapse2" class="accordion-body collapse "><div class="accordion-inner container-ae9f1139481542"><div class="container-ae9f1139481542 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h3> Understand how OncoPro medium streamlines media formulation and tumoroid culture </h3></div><div class="text parbase section"><p>OncoPro medium affords researchers workflow versatility by letting users decide if they want to utilize established embedded culture methods or transition to suspension methods to help save both time and resources.</p></div><div class="textimage parbase textimage-top section"><div class="image" id="cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481542tabtextimage_af58"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_af58/image.img.320.high.jpg/1732297881127.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_af58/image.img.480.high.jpg/1732297881127.jpg' media="(min-width: 490px)"><img class='textimage-auto' srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_af58/image.img.full.high.jpg/1732297881127.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_af58/image.img.320.high.jpg/1732297881127.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_af58/image.img.480.high.jpg/1732297881127.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_af58/image.img.320.medium.jpg/1732297881127.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_af58/image.img.620.high.jpg/1732297881127.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_af58/image.img.320.low.jpg/1732297881127.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_af58/image.img.480.medium.jpg/1732297881127.jpg" alt='Scientific diagrams showing tumoroid culture methods' title='OncoPro medium suspension culture simplifies tumoroid passaging'></picture></div><style> #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481542tabtextimage_af58 { max-width: 100%; } #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481542tabtextimage_af58 img { width: 100%; max-width: 579px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b>Figure 4. The OncoPro medium suspension culture method greatly simplifies tumoroid passaging, reducing hands-on time from 140 minutes for embedded culture to 60 minutes for suspension culture.</b> (A) Schematic of culture method steps for passaging tumoroids in embedded culture. Boxes highlight the pain points of disrupting extracellular matrix (ECM) domes, losing cells trapped in ECM during washing, and pipetting and waiting for ECM domes to solidify, respectively. (B) Schematic of culture method steps for passaging tumoroids in suspension culture, which addresses these pain points due to reduced ECM requirement.<br> Figure created with BioRender.com.</small></p></div></div><div class="clearfix"></div></div><div class="textimage parbase textimage-top section"><div class="image" id="cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481542tabtextimage_95dd"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_95dd/image.img.320.high.jpg/1732297881156.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_95dd/image.img.480.high.jpg/1732297881156.jpg' media="(min-width: 490px)"><img class='textimage-auto' srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_95dd/image.img.full.high.jpg/1732297881156.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_95dd/image.img.320.high.jpg/1732297881156.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_95dd/image.img.480.high.jpg/1732297881156.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_95dd/image.img.320.medium.jpg/1732297881156.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_95dd/image.img.620.high.jpg/1732297881156.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_95dd/image.img.320.low.jpg/1732297881156.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_95dd/image.img.480.medium.jpg/1732297881156.jpg" alt='Table comparing suspension vs embedded culture' title='OncoPro suspension cultures is ready to scale'></picture></div><style> #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481542tabtextimage_95dd { max-width: 100%; } #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481542tabtextimage_95dd img { width: 100%; max-width: 579px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b>Figure 5. Tumoroid cultures are readily scalable with the OncoPro medium suspension culture method.</b> The number and format of culture vessels required to obtain increasing scale of tumoroid cultures are more user- and resource-friendly in suspension culture when compared to traditional embedded culture methods. (A) Suspension methods allow for ready expansion of 100 million cells within one passage, whereas the number of basement membrane extract (BME) domes required in embedded culture would be prohibitive for many users. (B) Average amount of BME required to harvest 100 million cells for 9 tumoroid lines using different culture methods. On average, suspension culture required less BME than embedded culture (mean ± SEM, not significant).</small></p></div></div><div class="clearfix"></div></div><div class="textimage parbase textimage-left section"><div class="image" id="cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481542tabtextimage_2f3a"><a class="image-enlarge-link" data-lightbox="/content/lifetech/ipac/en-sg/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_2f3a" href="/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/_jcr_content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_2f3a/image.img.jpg/1732297881202.jpg"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_2f3a/image.img.full.high.jpg/1732297881186.jpg' media="(min-width: 1px)"><img class='textimage-auto' srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_2f3a/image.img.full.high.jpg/1732297881186.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_2f3a/image.img.320.high.jpg/1732297881186.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_2f3a/image.img.480.high.jpg/1732297881186.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_2f3a/image.img.320.medium.jpg/1732297881186.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_2f3a/image.img.620.high.jpg/1732297881186.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_2f3a/image.img.320.low.jpg/1732297881186.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481542/tab/textimage_2f3a/image.img.480.medium.jpg/1732297881186.jpg" alt='Correlation graph representing patient-specific gene expression' title='Patient-specific gene expression profiles'></picture></div><style> #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481542tabtextimage_2f3a { max-width: 100%; } #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481542tabtextimage_2f3a img { width: 100%; max-width: 426px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b>Figure 6. Patient-specific gene expression profiles are highly correlated between samples cultured using the streamlined suspension method and those cultured using traditional embedded methods. </b>Ward hierarchical clustering and Spearman’s ρ correlations of gene expression for a panel of 1423 cancer-related genes across a variety of tumoroid lines representing four different cancer indications. Gene expression assayed by Ion AmpliSeq Transcriptome Human Gene Expression Panel, Chef-Ready Kit. Figures adapted from JMP 16 software output. With the exception of colorectal tumoroid line Z, established tumoroid models provided by NCI PDMR. We thank the PDMR and their contributing institutions for their contributions to this work.</small></p></div></div><div class="clearfix"></div></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154" href="#collapse3"><span class="caret"></span><strong> Adaptable application </strong></a></div><div id="collapse3" class="accordion-body collapse "><div class="accordion-inner container-ae9f1139481543"><div class="container-ae9f1139481543 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h3> Learn how OncoPro medium functions as an adaptable system </h3></div><div class="text parbase section"><p>OncoPro Tumoroid Culture Medium Kit is a flexible system designed to address complex research needs. OncoPro medium accommodates multiple cancer indications using a single media kit and the scalable and automation-friendly suspension workflow facilitates smooth transitions to various downstream assays. The absence of Wnt, R-spondin, Noggin, and small molecule inhibitors make OncoPro well-suited for advanced biological studies and screening applications.</p></div><div class="textimage parbase textimage-left section"><div class="image" id="cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481543tabtextimage_905e"><a class="image-enlarge-link" data-lightbox="/content/lifetech/ipac/en-sg/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_905e" href="/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/_jcr_content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_905e/backgroundimg.img.jpg/1732297881271.jpg"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_905e/image.img.full.high.jpg/1732297881249.jpg' media="(min-width: 1px)"><img class='textimage-auto' srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_905e/image.img.full.high.jpg/1732297881249.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_905e/image.img.320.high.jpg/1732297881249.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_905e/image.img.480.high.jpg/1732297881249.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_905e/image.img.320.medium.jpg/1732297881249.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_905e/image.img.620.high.jpg/1732297881249.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_905e/image.img.320.low.jpg/1732297881249.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_905e/image.img.480.medium.jpg/1732297881249.jpg" alt='Images of tumoroids' title='Tumoroid morphology comparison'></picture></div><style> #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481543tabtextimage_905e { max-width: 100%; } #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481543tabtextimage_905e img { width: 100%; max-width: 426px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><small><b>Figure 7. OncoPro medium is compatible with four different cancer indications, for both suspension and embedded culture.</b> Morphology of NCI PDMR tumoroid lines expanded in NCI PDMR homebrew media (embedded culture), or in OncoPro medium embedded or suspension culture. Scale bar = 400 µm. Established tumoroid models provided by NCI PDMR. We thank the PDMR and their contributing institutions for their contributions to this work.</small></div></div><div class="clearfix"></div></div><div class="textimage parbase textimage-left section"><div class="image" id="cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481543tabtextimage_ddc6"><a class="image-enlarge-link" data-lightbox="/content/lifetech/ipac/en-sg/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_ddc6" href="/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/_jcr_content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_ddc6/backgroundimg.img.jpg/1732297881300.jpg"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_ddc6/image.img.full.high.jpg/1732297881279.jpg' media="(min-width: 1px)"><img class='textimage-auto' srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_ddc6/image.img.full.high.jpg/1732297881279.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_ddc6/image.img.320.high.jpg/1732297881279.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_ddc6/image.img.480.high.jpg/1732297881279.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_ddc6/image.img.320.medium.jpg/1732297881279.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_ddc6/image.img.620.high.jpg/1732297881279.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_ddc6/image.img.320.low.jpg/1732297881279.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_ddc6/image.img.480.medium.jpg/1732297881279.jpg" alt='Graphs of tumoroid population doubling vs days' title='Tumoroid doubling time evaluation'></picture></div><style> #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481543tabtextimage_ddc6 { max-width: 100%; } #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481543tabtextimage_ddc6 img { width: 100%; max-width: 426px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b>Figure 8. OncoPro medium is compatible with four different cancer indications, for both suspension and embedded culture. </b>Cumulative population doublings of cells expanded in OncoPro medium suspension culture (purple), OncoPro medium embedded culture (red), or alternative media system (media system in which the line was derived prior to procurement) embedded culture (green). In general, tumoroids expanded in each culture condition with comparable growth rates. With the exception of colorectal tumoroid line Z, established tumoroid models provided by NCI PDMR. We thank the PDMR and their contributing institutions for their contributions to this work.</small></p></div></div><div class="clearfix"></div></div><div class="textimage parbase textimage-left section"><div class="image" id="cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481543tabtextimage_69d7"><a class="image-enlarge-link" data-lightbox="/content/lifetech/ipac/en-sg/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_69d7" href="/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/_jcr_content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_69d7/image.img.jpg/1732297881324.jpg"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_69d7/image.img.full.high.jpg/1732297881308.jpg' media="(min-width: 1px)"><img class='textimage-auto' srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_69d7/image.img.full.high.jpg/1732297881308.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_69d7/image.img.320.high.jpg/1732297881308.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_69d7/image.img.480.high.jpg/1732297881308.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_69d7/image.img.320.medium.jpg/1732297881308.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_69d7/image.img.620.high.jpg/1732297881308.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_69d7/image.img.320.low.jpg/1732297881308.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/tabworkflowcontainer_113948154/container-ae9f1139481543/tab/textimage_69d7/image.img.480.medium.jpg/1732297881308.jpg" alt='Correlation graph representing patient-specific gene expression' title='Patient-specific gene expression profiles'></picture></div><style> #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481543tabtextimage_69d7 { max-width: 100%; } #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_113948154container-ae9f1139481543tabtextimage_69d7 img { width: 100%; max-width: 426px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><p><small><b>Figure 9. OncoPro medium helps maintain patient-specific gene expression profiles from lines derived in alternative media and embedded culture. </b>Principal component analysis (PCA) plot comparing gene expression levels across tumoroid lines and culture conditions. Tumoroid lines were expanded in parallel in multiple media and culture conditions for 2-7 passages post-thaw prior to quantification of expression levels across over >20,000 human RefSeq genes using the Ion AmpliSeq Transcriptome Human Gene Expression Kit. Tumoroid lines tested were 782815-120-R-V1-organoid (Colorectal A), 919269-233-R3-V2-organoid (Colorectal B), 349418-098-R-V2-organoid (Lung), 549293-155-R-V1-organoid (Head and Neck), and 699152-130-R-V1-organoid (Pancreas). With the exception of colorectal tumoroid line Z, established tumoroid models were provided by NCI PDMR. We thank the PDMR and their contributing institutions for their contributions to this work. Figure adapted from TAC 4.0 software output.</small></p></div></div><div class="clearfix"></div></div></div><div class="end"></div></div></div></div></div></div></div><div class="well-cmp section"><div class="well well-blue-light well-default"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="text parbase section"><h3>Looking for more information about OncoPro Tumoroid Culture Medium or how you can request a sample quote?</h3><p><a href="/sg/en/home/global/forms/oncopro-medium-info.html" class="cta-link">Contact us</a></p></div></div></div></div></div></div><div class="heading section"><h2 id="literature"><br>Explore technical resources demonstrating the performance and application of OncoPro Medium </h2></div><div class="tabworkflowcontainer section"><div class="tabbable plain-tab"><ul class="nav nav-tabs" id="myTab"><li class="active"><a data-toggle="tab" href="#container-ae9f2">Application notes</a></li><li><a data-toggle="tab" href="#container-ae9f1">Posters</a></li><li><a data-toggle="tab" href="#container-ae9f12830801153">Webinars</a></li></ul><div class="tab-content" id="myTabContent"><div id="container-ae9f2" class="tab-pane active"><div class="container-ae9f2 tabstep"><div class="start"></div><div class="tab parsys"><div class="text parbase section"><div class="table-component-wrapper"><table border="1" cellspacing="0" cellpadding="0" class="rte-table" id="table-367385290"><tbody><tr><th width="312" valign="top">Application note title</th><th width="312" valign="top">Description</th></tr><tr><td width="312" valign="top"><a href="https://assets.thermofisher.com/TFS-Assets/BID/Application-Notes/derived-tumoroids-cancer-research-app-note.pdf" target="_blank">Engineering patient-derived tumoroids to help advance cancer research</a></td><td width="312" valign="top"><ul><li>Explore advanced methods for overexpressing and downregulating genes in patient-derived colorectal cancer tumoroids using lipofection, electroporation, and lentiviral transduction.</li><li>Discover how to achieve optimal gene delivery with practical recommendations and troubleshooting tips to maximize payload uptake and maintain cell viability.</li></ul></td></tr><tr><td width="312" valign="top"><a href="https://assets.thermofisher.com/TFS-Assets/BID/Application-Notes/oncopro-tumoroid-culture-medium-app-note.pdf" target="_blank">Tumoroid line derivation using OncoPro Tumoroid Culture Medium</a></td><td width="312" valign="top"><ul><li>Demonstrates that OncoPro Medium supports derivation of new tumoroid lines from clinical samples, such as tumor resections or patient-derived xenograft (PDX) tissues</li><li>Addresses factors affecting derivation success rates (e.g., tissue quality, fresh vs frozen samples, etc.)</li><li>Provides best practices for tissue handling, dissociation, and culture initiation when deriving new tumoroids</li></ul></td></tr><tr><td width="312" valign="top"><p><a href="https://assets.thermofisher.com/TFS-Assets/BID/Application-Notes/breast-cancer-tumoroid-culture-medium-app-note.pdf" target="_blank">Culture of triple-negative breast cancer tumoroid lines in OncoPro Tumoroid Culture Medium</a></p></td><td width="312" valign="top"><ul><li>Demonstrates OncoPro Medium compatibility with triple-negative breast cancer (TNBC) tumoroids</li><li>Recommended indication specific growth factors: heat-stable FGF10 and beta-estradiol</li><li>Maintenance of patient-specific characteristics including morphology, growth rates, and mutational and transcriptional profiles</li></ul></td></tr><tr><td width="312" valign="top"><a href="https://assets.thermofisher.com/TFS-Assets/BID/Application-Notes/screens-oncoPro-tumoroid-culture-medium-app-note.pdf" target="_blank">Enabling tumoroid-based compound screens with OncoPro Tumoroid Culture Medium</a></td><td width="312" valign="top"><ul><li>Highlights a) value of tumoroids over traditional immortalized cell lines for drug screening and b) how OncoPro suspension protocol enables high throughput workflows</li><li>Addresses practical considerations such as scaling up cultures, ensuring reproducible tumoroid plating, and designing multiplexed assay readouts.</li></ul></td></tr></tbody></table></div></div></div><div class="end"></div></div></div><div id="container-ae9f1" class="tab-pane"><div class="container-ae9f1 tabstep"><div class="start"></div><div class="tab parsys"><div class="text parbase section"><p>Access five unique posters from our research and development scientists that highlight the key features of OncoPro medium and how it can be applied in certain tumoroid culture workflows such as drug screening and immuno-oncology. </p></div><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" id="table--1249942020"><tbody><tr><th width="50%">Poster Title</th><th>Description</th></tr><tr><td><p><a href="https://assets.thermofisher.com/TFS-Assets/BID/posters/derivation-maintenance-patient-derived-tumoroid-lines-poster.pdf" target="_blank"><b>Derivation & long-term maintenance of patient-derived tumoroid lines in a defined, serum-free medium</b></a></p></td><td><ul><li>Maintenance of growth rates, patient phenotype and genotype for ≥40 passages</li><li>Support for multiple cancer indications using tissue-specific growth factors</li><li>Scale up to >1 billion cancer organoid cells with easy-to-use suspension method</li></ul></td></tr><tr><td><p><a href="https://assets.thermofisher.com/TFS-Assets/BID/posters/tumoroids-wnt-agonist-free-media-system-poster.pdf" target="_blank"><b>Expansion of established patient-derived tumoroids in a novel serum-free, Wnt agonist-free media system</b></a></p></td><td><ul><li>Long-term maintenance of mutational status and gene expression levels in both embedded and suspension formats</li><li>Wnt-related signaling pathways are not differentially activated following culture in Wnt agonist-free OncoPro Tumoroid Culture Medium</li></ul></td></tr><tr><td><p><a href="https://assets.thermofisher.com/TFS-Assets/BID/posters/maintenance-tumoroid-phenotype-highly-scalable-reduced-ecm-suspension-culture-method-poster.pdf" target="_blank"><b>How low can you go: Maintenance of tumoroid phenotype with a highly scalable and automation-compatible reduced-ECM suspension culture method</b></a></p></td><td><ul><li>Suspension culture using OncoPro medium is compatible with existing tumoroid lines</li><li>Patient phenotype and genotype in suspension conditions using OncoPro medium are comparable with embedded method</li></ul></td></tr><tr><td><p><a href="https://assets.thermofisher.com/TFS-Assets/BID/posters/multiplexed-plate-reader-drug-screening-3d-tumoroid-models-poster.pdf" target="_blank"><b>Multiplexed plate-reader based drug screening of 3D-tumoroid models</b></a></p></td><td><ul><li>Development and optimization of drug screening assay using tumoroids</li><li>Drug response comparison of tumoroids and immortalized cell lines 2D and 3D</li><li>Compound screening application using tumoroids</li></ul></td></tr><tr><td><a href="https://assets.thermofisher.com/TFS-Assets/BID/posters/engineering-patient-tumoroids-immuno-oncology-workflows-poster.pdf" target="_blank"><b>Engineering patient-derived tumoroids to enable high-throughput screening:</b><b>Immuno-oncology workflows</b></a></td><td><ul><li>Establishment of GFP-expressing tumoroid reporter pool</li><li>Demonstration of NK-cell mediated cytotoxicity against tumoroids</li><li>Comparison of primary, iPSC-derived, and NK lines cytotoxicity</li></ul></td></tr></tbody></table></div></div></div><div class="end"></div></div></div><div id="container-ae9f12830801153" class="tab-pane"><div class="container-ae9f12830801153 tabstep"><div class="start"></div><div class="tab parsys"><div class="text parbase section"><p><b>Webinar:</b> Engineering patient-derived tumoroids to enable high-throughput screening for immuno-oncology workflows</p><p>Patient-derived tumoroids, commonly referred to as cancer organoids, represent a promising avenue as cost-effective and biologically relevant cancer immunotherapy research models. In this study, we developed a proof-of-concept immunotherapy screening platform harnessing tumoroids to validate the cytotoxic activity of Natural Killer (NK) cells. Tumoroids were genetically engineered to create a GFP-expressing reporter pool that could be used to assess killing efficiency using live cell imaging platforms. We were able to demonstrate dose-dependent tumoroid killing with increasing effector-to-target ratios. This innovative approach holds the potential to streamline the engineering of patient-derived tumoroid models, facilitate the development of multiplex-killing assays, and provide an efficient tool for evaluating immunotherapies targeting solid tumors.</p><p><b>Speaker:</b></p><div class="table-component-wrapper"><table cellpadding="0" cellspacing="0" border="0" id="table--1668319291"><tbody><tr><td style="text-align: left;"><img src="/content/dam/LifeTech/global/life-sciences/bid/cell-biology/2024/05/garrett-wong.jpg" title="Garret Wong photo" alt="Garret Wong photo" style="max-width: 150px;"></td><td> </td></tr></tbody></table></div><p>Garrett Wong, MS, Manager of Cell Biology Services, Thermo Fisher Scientific</p><p><b>Learning objectives:</b></p><ul><li>Explore the applications of tumoroids in cancer immunotherapy workflows</li><li>Establish and engineer patient-derived reporter tumoroid models</li><li>Develop and optimize assays for immunotherapy screens using tumoroid co-cultures</li></ul><p><span class="btn btn-primary"><a href="https://www.labroots.com/ms/webinar/engineering-patient-derived-tumoroids-enable-high-throughput-screening-immuno-oncology-workflows?campaign=IOwebinar" target="_blank">Watch now</a></span></p><p> </p></div><div class="text parbase section"><p><b>Webinar: </b>Advancing cancer organoid drug screening with novel media systems</p><p>Explore the latest developments in cancer organoid (tumoroid) technology and the promising implications for drug screening during this two-part webinar. In the first half, Colin Paul discusses the capabilities of OncoPro medium, a novel system that simplifies the use of tumoroids for drug screening applications. This session reviews potential areas of optimization and highlights how OncoPro medium mitigates these challenges. In the second half, Christophe Deben from Orbits leads us through the cutting edge of imaging software. He demonstrates how their technology effectively resolves downstream bottlenecks in high-content image analysis, providing invaluable insights into the translation of tumoroid and cancer organoid technology for high-throughput applications. Watch these presentations and gain a deeper understanding of the current progress and future direction of tumoroid-based drug screening and image analysis techniques.<b></b></p><p><b>Speakers:</b></p><div class="table-component-wrapper"><table cellpadding="0" cellspacing="0" border="0" id="table-1153560498"><tbody><tr><td style="text-align: left;" width="30%"><img src="/content/dam/LifeTech/global/life-sciences/bid/cell-biology/2024/05/christophe-deben.jpg" title="Dr. Christophe Deben photo" alt="Dr. Christophe Deben photo" style="max-width: 150px;"><br><b>Dr. Christophe Deben</b>,<br> Ph.D. Oncology and Cancer Biology, Professor Tumor Biology and Organoids, University of Antwerp<br>  </td><td style="text-align: left;" width="20%"><img src="/content/dam/LifeTech/global/life-sciences/bid/cell-biology/2024/05/colin-paul.jpg" title="Dr. Colin Paul photo" alt="Dr. Colin Paul photo" style="max-width: 150px;"><br><b>Dr. Colin Paul</b>,<br> Cell Biology R&D Staff Scientist, Thermo Fisher Scientific</td><td> </td><td> </td><td> </td></tr></tbody></table></div><p><b>Learning objectives:</b></p><ul><li>Learn how cancer organoids can be adopted for high-throughput screening applications</li><li>Understand areas of optimization in drug screening workflows for improved consistency and reproducibility</li><li>Learn how image analysis can be improved to better correlate assay results with patient outcomes</li></ul><p><span class="btn btn-primary"><a href="https://www.labroots.com/ms/webinar/advancing-cancer-organoid-drug-screening-novel-media-systems?campaign=0524oncoprowebinar" target="_blank">Watch now</a></span></p></div></div><div class="end"></div></div></div></div></div><div class="workflow-accordion accordion" id="accordion-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_1283080115"><div class="accordion-group"><div class="accordion-heading active"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_1283080115" href="#collapse1"><span class="caret"></span><strong> Application notes </strong></a></div><div id="collapse1" class="accordion-body collapse in "><div class="accordion-inner container-ae9f2"><div class="container-ae9f2 tabstep"><div class="start"></div><div class="tab parsys"><div class="text parbase section"><div class="table-component-wrapper"><table border="1" cellspacing="0" cellpadding="0" class="rte-table" id="table--307614627"><tbody><tr><th width="312" valign="top">Application note title</th><th width="312" valign="top">Description</th></tr><tr><td width="312" valign="top"><a href="https://assets.thermofisher.com/TFS-Assets/BID/Application-Notes/derived-tumoroids-cancer-research-app-note.pdf" target="_blank">Engineering patient-derived tumoroids to help advance cancer research</a></td><td width="312" valign="top"><ul><li>Explore advanced methods for overexpressing and downregulating genes in patient-derived colorectal cancer tumoroids using lipofection, electroporation, and lentiviral transduction.</li><li>Discover how to achieve optimal gene delivery with practical recommendations and troubleshooting tips to maximize payload uptake and maintain cell viability.</li></ul></td></tr><tr><td width="312" valign="top"><a href="https://assets.thermofisher.com/TFS-Assets/BID/Application-Notes/oncopro-tumoroid-culture-medium-app-note.pdf" target="_blank">Tumoroid line derivation using OncoPro Tumoroid Culture Medium</a></td><td width="312" valign="top"><ul><li>Demonstrates that OncoPro Medium supports derivation of new tumoroid lines from clinical samples, such as tumor resections or patient-derived xenograft (PDX) tissues</li><li>Addresses factors affecting derivation success rates (e.g., tissue quality, fresh vs frozen samples, etc.)</li><li>Provides best practices for tissue handling, dissociation, and culture initiation when deriving new tumoroids</li></ul></td></tr><tr><td width="312" valign="top"><p><a href="https://assets.thermofisher.com/TFS-Assets/BID/Application-Notes/breast-cancer-tumoroid-culture-medium-app-note.pdf" target="_blank">Culture of triple-negative breast cancer tumoroid lines in OncoPro Tumoroid Culture Medium</a></p></td><td width="312" valign="top"><ul><li>Demonstrates OncoPro Medium compatibility with triple-negative breast cancer (TNBC) tumoroids</li><li>Recommended indication specific growth factors: heat-stable FGF10 and beta-estradiol</li><li>Maintenance of patient-specific characteristics including morphology, growth rates, and mutational and transcriptional profiles</li></ul></td></tr><tr><td width="312" valign="top"><a href="https://assets.thermofisher.com/TFS-Assets/BID/Application-Notes/screens-oncoPro-tumoroid-culture-medium-app-note.pdf" target="_blank">Enabling tumoroid-based compound screens with OncoPro Tumoroid Culture Medium</a></td><td width="312" valign="top"><ul><li>Highlights a) value of tumoroids over traditional immortalized cell lines for drug screening and b) how OncoPro suspension protocol enables high throughput workflows</li><li>Addresses practical considerations such as scaling up cultures, ensuring reproducible tumoroid plating, and designing multiplexed assay readouts.</li></ul></td></tr></tbody></table></div></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_1283080115" href="#collapse2"><span class="caret"></span><strong> Posters </strong></a></div><div id="collapse2" class="accordion-body collapse "><div class="accordion-inner container-ae9f1"><div class="container-ae9f1 tabstep"><div class="start"></div><div class="tab parsys"><div class="text parbase section"><p>Access five unique posters from our research and development scientists that highlight the key features of OncoPro medium and how it can be applied in certain tumoroid culture workflows such as drug screening and immuno-oncology. </p></div><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" id="table--824920391"><tbody><tr><th width="50%">Poster Title</th><th>Description</th></tr><tr><td><p><a href="https://assets.thermofisher.com/TFS-Assets/BID/posters/derivation-maintenance-patient-derived-tumoroid-lines-poster.pdf" target="_blank"><b>Derivation & long-term maintenance of patient-derived tumoroid lines in a defined, serum-free medium</b></a></p></td><td><ul><li>Maintenance of growth rates, patient phenotype and genotype for ≥40 passages</li><li>Support for multiple cancer indications using tissue-specific growth factors</li><li>Scale up to >1 billion cancer organoid cells with easy-to-use suspension method</li></ul></td></tr><tr><td><p><a href="https://assets.thermofisher.com/TFS-Assets/BID/posters/tumoroids-wnt-agonist-free-media-system-poster.pdf" target="_blank"><b>Expansion of established patient-derived tumoroids in a novel serum-free, Wnt agonist-free media system</b></a></p></td><td><ul><li>Long-term maintenance of mutational status and gene expression levels in both embedded and suspension formats</li><li>Wnt-related signaling pathways are not differentially activated following culture in Wnt agonist-free OncoPro Tumoroid Culture Medium</li></ul></td></tr><tr><td><p><a href="https://assets.thermofisher.com/TFS-Assets/BID/posters/maintenance-tumoroid-phenotype-highly-scalable-reduced-ecm-suspension-culture-method-poster.pdf" target="_blank"><b>How low can you go: Maintenance of tumoroid phenotype with a highly scalable and automation-compatible reduced-ECM suspension culture method</b></a></p></td><td><ul><li>Suspension culture using OncoPro medium is compatible with existing tumoroid lines</li><li>Patient phenotype and genotype in suspension conditions using OncoPro medium are comparable with embedded method</li></ul></td></tr><tr><td><p><a href="https://assets.thermofisher.com/TFS-Assets/BID/posters/multiplexed-plate-reader-drug-screening-3d-tumoroid-models-poster.pdf" target="_blank"><b>Multiplexed plate-reader based drug screening of 3D-tumoroid models</b></a></p></td><td><ul><li>Development and optimization of drug screening assay using tumoroids</li><li>Drug response comparison of tumoroids and immortalized cell lines 2D and 3D</li><li>Compound screening application using tumoroids</li></ul></td></tr><tr><td><a href="https://assets.thermofisher.com/TFS-Assets/BID/posters/engineering-patient-tumoroids-immuno-oncology-workflows-poster.pdf" target="_blank"><b>Engineering patient-derived tumoroids to enable high-throughput screening:</b><b>Immuno-oncology workflows</b></a></td><td><ul><li>Establishment of GFP-expressing tumoroid reporter pool</li><li>Demonstration of NK-cell mediated cytotoxicity against tumoroids</li><li>Comparison of primary, iPSC-derived, and NK lines cytotoxicity</li></ul></td></tr></tbody></table></div></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystabworkflowcontainer_1283080115" href="#collapse3"><span class="caret"></span><strong> Webinars </strong></a></div><div id="collapse3" class="accordion-body collapse "><div class="accordion-inner container-ae9f12830801153"><div class="container-ae9f12830801153 tabstep"><div class="start"></div><div class="tab parsys"><div class="text parbase section"><p><b>Webinar:</b> Engineering patient-derived tumoroids to enable high-throughput screening for immuno-oncology workflows</p><p>Patient-derived tumoroids, commonly referred to as cancer organoids, represent a promising avenue as cost-effective and biologically relevant cancer immunotherapy research models. In this study, we developed a proof-of-concept immunotherapy screening platform harnessing tumoroids to validate the cytotoxic activity of Natural Killer (NK) cells. Tumoroids were genetically engineered to create a GFP-expressing reporter pool that could be used to assess killing efficiency using live cell imaging platforms. We were able to demonstrate dose-dependent tumoroid killing with increasing effector-to-target ratios. This innovative approach holds the potential to streamline the engineering of patient-derived tumoroid models, facilitate the development of multiplex-killing assays, and provide an efficient tool for evaluating immunotherapies targeting solid tumors.</p><p><b>Speaker:</b></p><div class="table-component-wrapper"><table cellpadding="0" cellspacing="0" border="0" id="table-1907622069"><tbody><tr><td style="text-align: left;"><img src="/content/dam/LifeTech/global/life-sciences/bid/cell-biology/2024/05/garrett-wong.jpg" title="Garret Wong photo" alt="Garret Wong photo" style="max-width: 150px;"></td><td> </td></tr></tbody></table></div><p>Garrett Wong, MS, Manager of Cell Biology Services, Thermo Fisher Scientific</p><p><b>Learning objectives:</b></p><ul><li>Explore the applications of tumoroids in cancer immunotherapy workflows</li><li>Establish and engineer patient-derived reporter tumoroid models</li><li>Develop and optimize assays for immunotherapy screens using tumoroid co-cultures</li></ul><p><span class="btn btn-primary"><a href="https://www.labroots.com/ms/webinar/engineering-patient-derived-tumoroids-enable-high-throughput-screening-immuno-oncology-workflows?campaign=IOwebinar" target="_blank">Watch now</a></span></p><p> </p></div><div class="text parbase section"><p><b>Webinar: </b>Advancing cancer organoid drug screening with novel media systems</p><p>Explore the latest developments in cancer organoid (tumoroid) technology and the promising implications for drug screening during this two-part webinar. In the first half, Colin Paul discusses the capabilities of OncoPro medium, a novel system that simplifies the use of tumoroids for drug screening applications. This session reviews potential areas of optimization and highlights how OncoPro medium mitigates these challenges. In the second half, Christophe Deben from Orbits leads us through the cutting edge of imaging software. He demonstrates how their technology effectively resolves downstream bottlenecks in high-content image analysis, providing invaluable insights into the translation of tumoroid and cancer organoid technology for high-throughput applications. Watch these presentations and gain a deeper understanding of the current progress and future direction of tumoroid-based drug screening and image analysis techniques.<b></b></p><p><b>Speakers:</b></p><div class="table-component-wrapper"><table cellpadding="0" cellspacing="0" border="0" id="table--103330402"><tbody><tr><td style="text-align: left;" width="30%"><img src="/content/dam/LifeTech/global/life-sciences/bid/cell-biology/2024/05/christophe-deben.jpg" title="Dr. Christophe Deben photo" alt="Dr. Christophe Deben photo" style="max-width: 150px;"><br><b>Dr. Christophe Deben</b>,<br> Ph.D. Oncology and Cancer Biology, Professor Tumor Biology and Organoids, University of Antwerp<br>  </td><td style="text-align: left;" width="20%"><img src="/content/dam/LifeTech/global/life-sciences/bid/cell-biology/2024/05/colin-paul.jpg" title="Dr. Colin Paul photo" alt="Dr. Colin Paul photo" style="max-width: 150px;"><br><b>Dr. Colin Paul</b>,<br> Cell Biology R&D Staff Scientist, Thermo Fisher Scientific</td><td> </td><td> </td><td> </td></tr></tbody></table></div><p><b>Learning objectives:</b></p><ul><li>Learn how cancer organoids can be adopted for high-throughput screening applications</li><li>Understand areas of optimization in drug screening workflows for improved consistency and reproducibility</li><li>Learn how image analysis can be improved to better correlate assay results with patient outcomes</li></ul><p><span class="btn btn-primary"><a href="https://www.labroots.com/ms/webinar/advancing-cancer-organoid-drug-screening-novel-media-systems?campaign=0524oncoprowebinar" target="_blank">Watch now</a></span></p></div></div><div class="end"></div></div></div></div></div></div></div><div class="heading section"><h2 id="order"><br>Order your OncoPro Tumoroid Culture Medium Kit </h2></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt2"><div class="parsys_column cq-colctrl-lt2-c0"><div container-id="cq-colctrl-lt2" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsysimage_dde5"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/image_dde5/foregroundimg.img.320.high.jpg/1732297881495.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/image_dde5/foregroundimg.img.480.high.jpg/1732297881495.jpg' media="(min-width: 490px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/image_dde5/foregroundimg.img.full.high.jpg/1732297881495.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/image_dde5/foregroundimg.img.320.high.jpg/1732297881495.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/image_dde5/foregroundimg.img.480.high.jpg/1732297881495.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/image_dde5/foregroundimg.img.320.medium.jpg/1732297881495.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/image_dde5/foregroundimg.img.620.high.jpg/1732297881495.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/image_dde5/foregroundimg.img.320.low.jpg/1732297881495.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/image_dde5/foregroundimg.img.480.medium.jpg/1732297881495.jpg" alt='OncoPro product photo' title='OncoPro Tumoroid Culture Medium Ki'></picture><style> #cq-image-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsysimage_dde5 img{ width:100%; max-width: 600px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"5b206439-5546-4669-9a5e-dca44a4c347dMainParsysimage_dde5 .image-contain" + "," + "#cq-image-jsp-"+"5b206439-5546-4669-9a5e-dca44a4c347dMainParsysimage_dde5 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div><div class="parsys_column cq-colctrl-lt2-c1"><div container-id="cq-colctrl-lt2" comp-id="B1"><div class="productlist parbase section"><div id="skulist" style="display:none">A5701201</div><div id='ltajax-9173034487937288028'></div><script type="text/javascript">(function() {$('#ltajax-9173034487937288028').load('/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/productlist_a682.ajax.html?skulist=');})(); </script></div></div></div></div><div style="clear:both"></div><div class="heading section"><h2> OncoPro Tumoroid Culture Medium recommended reagents </h2></div><div class="text parbase section"><p>The reagents below are recommended for culturing cancer organoids using OncoPro Tumoroid Culture Medium Kit.</p><p>We have demonstrated the OncoPro Tumoroid Culture Medium Kit supports the culture of several epithelial-derived tumoroids, but additional growth factors may be required for some indications.</p><ul><li><b>Colorectal tumoroids:</b> No additional growth factors needed.</li><li><b>Lung and head & neck tumoroids: </b><a href="/proteins/product/Heat-Stable-FGF-10-Recombinant-Protein/PHG0372">Heat stable FGF-10</a> required.</li><li><b>Pancreatic tumoroids: </b><a href="/proteins/product/Heat-Stable-FGF-10-Recombinant-Protein/PHG0372">Heat stable FGF-10</a> and <a href="https://www.fishersci.com/shop/products/tocris-gastrin-i-human/30061">Gastrin I (human) (Cat. No. 30-061 from Fisher Scientific)</a>.</li><li><b>Breast (triple negative breast cancer) and endometrial: </b><a href="/proteins/product/Heat-Stable-FGF-10-Recombinant-Protein/PHG0372">Heat stable FGF-10</a> and <a href="https://www.fishersci.com/shop/products/beta-estradiol-powder-gamma-i/501848155">Beta-Estradiol (Cat. No. 5018488155 from Fisher Scientific)</a>.</li></ul><p>For other indications, please <a href="/us/en/home/technical-resources/contact-us.html">Contact our support team</a> to learn how you may be able to use OncoPro medium.</p></div><div class="accordion section"><div class="accordion" id="bbbf44de241c4361bafbdb27e1c60915" style='margin-bottom: 0;'><div class="parsys"><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#bbbf44de241c4361bafbdb27e1c60915" href="#order1"><span class="caret"></span> Other recommended reagents and consumables </a></div><div class="accordion-body collapse " id="order1"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="text parbase section"><h3>The following recommended reagents can be purchased directly from the Fisher Scientific catalog: </h3><ul><li>Gastrin I (human), required for pancreatic tumoroids only (<a href="https://www.fishersci.com/shop/products/tocris-gastrin-i-human/30061">Fisher Scientific, Cat. No. 30-061</a>)</li><li>Beta-Estradiol, required for breast (triple negative breast cancer) and endometrial tumoroids only (<a href="https://www.fishersci.com/shop/products/beta-estradiol-powder-gamma-i/501848155">Fisher Scientific, Cat. No. 501848155</a>)</li><li>InvivoGen Primocin™ (<a href="https://www.fishersci.com/shop/products/primocin-1-g-50-mg-ml/NC9392943">Fisher Scientific, Cat. No. NC9392943</a>)</li><li>Y-27632 2HCl (<a href="https://www.fishersci.com/shop/products/y-27632-2hcl-y27632-99-1/508636">Fisher Scientific, Cat. No. 50-863-6</a>)</li></ul></div><div class="heading section"><h3> Recommended reagents from Thermo Fisher Scientific </h3></div><div class="productlist parbase section"><div id="skulist" style="display:none">PHG0371,PHG0372,PHG0373,A1413201,A1413202,A1110501,150239,169900,156800,10565018,15140122,15260037,14190144,12648010</div><div id='ltajax-5428426123095147388'></div><script type="text/javascript">(function() {$('#ltajax-5428426123095147388').load('/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/accordion_eddd/itemspar/accordionitem_d2a0/itemParsys/productlist_20c3.ajax.html?skulist=');})(); </script></div><div class="well-cmp section"><div class="well well-gray-lighter well-default"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="text parbase section"><h4>Thermo Fisher Scientific also offers Gibco OncoPro Tumoroid Cell Lines for purchase. Utilize our general inquiry form below to get a quote and additional information.</h4><p><a class="cta-link" href="/sg/en/home/global/forms/how-can-our-scientists-help-you.html">Submit an inquiry</a></p></div></div></div></div></div></div></div></div></div></div></div></div></div></div><script> $j(document).ready(function(){ $j('#bbbf44de241c4361bafbdb27e1c60915').on('hide', function(e) { $(e.target).find(".accordion-content").hide(); }); $j('#bbbf44de241c4361bafbdb27e1c60915').on('show', function(e) { $(e.target).find(".accordion-content").show(); }); }); </script></div><div class="heading section"><h2><br>Blog articles </h2></div><div class="text parbase section"><ul class="the-icons clearfix"><li><a href="/blog/behindthebench/co-culturing-patient-derived-cancer-organoids-with-fibroblasts-and-endothelial-cells?cid=bid_clb_can_r01_co_cp1610_pjt12005_col000000_0wm_tfs_il_awa_og_s00_" class="cta-link">Co-culturing patient-derived cancer organoids with fibroblasts and endothelial cells</a></li><li><a href="/blog/behindthebench/cancer-organoids-vs-cancer-spheroids/?cid=bid_clb_can_r01_co_cp1610_pjt12005_col000000_0wm_tfs_il_awa_og_s00_" class="cta-link">Cancer organoids vs cancer spheroids</a></li><li><a href="/blog/behindthebench/how-3d-cell-culture-models-work?cid=bid_clb_can_r01_co_cp1610_pjt12005_col000000_0wm_tfs_il_awa_og_s00_" class="cta-link">How 3D cell culture models work</a></li><li><a href="/blog/behindthebench/cancer-organoids-in-car-t-cell-therapy-research/?cid=bid_clb_can_r01_co_cp1610_pjt12005_col000000_0wm_tfs_il_awa_og_s00_" class="cta-link">Cancer organoids in CAR T cell therapy research</a></li></ul></div><div class="heading section"><h2 id="resources"><br>Engage with additional resources for tumoroid culture </h2></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="textimage parbase section"><div class="image" id="cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystextimage_bca4"><a href="/sg/en/home/products-and-services/services/cell-biology-services/tumoroid-services.html"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_bca4/image.img.full.high.jpg/1732297881652.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_bca4/image.img.full.high.jpg/1732297881652.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_bca4/image.img.320.high.jpg/1732297881652.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_bca4/image.img.480.high.jpg/1732297881652.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_bca4/image.img.320.medium.jpg/1732297881652.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_bca4/image.img.620.high.jpg/1732297881652.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_bca4/image.img.320.low.jpg/1732297881652.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_bca4/image.img.480.medium.jpg/1732297881652.jpg" alt='Scientists with a tumoroid' title='Tumoroid Assay Services'></picture></div><style> #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystextimage_bca4 { max-width: 100%; } #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystextimage_bca4 img { width: 100%; max-width: 426px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><h3><a adhocenable="false" href="/sg/en/home/products-and-services/services/cell-biology-services/tumoroid-services.html">Tumoroid Assay Services</a></h3><p>Learn how our team of experts can help tumoroid researchers with workflow optimization or transition support.</p></div></div><div class="clearfix"></div></div></div><div container-id="cq-colctrl-lt0" comp-id="A2"><div class="textimage parbase section"><div class="image" id="cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystextimage_50af"><a href="/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture.html"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_50af/image.img.full.high.jpg/1732297881684.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_50af/image.img.full.high.jpg/1732297881684.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_50af/image.img.320.high.jpg/1732297881684.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_50af/image.img.480.high.jpg/1732297881684.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_50af/image.img.320.medium.jpg/1732297881684.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_50af/image.img.620.high.jpg/1732297881684.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_50af/image.img.320.low.jpg/1732297881684.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_50af/image.img.480.medium.jpg/1732297881684.jpg" alt='decorative image' title='Tumoroid culture'></picture></div><style> #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystextimage_50af { max-width: 100%; } #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystextimage_50af img { width: 100%; max-width: 426px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><h3><a href="/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture.html">Tumoroid Culture</a></h3><p>Visit our tumoroid webpage to learn about these models and how they can be utilized in cancer research.</p></div></div><div class="clearfix"></div></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="textimage parbase section"><div class="image" id="cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystextimage_afb4"><a href="/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-cell-lines.html"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_afb4/image.img.full.high.jpg/1732297881719.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_afb4/image.img.full.high.jpg/1732297881719.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_afb4/image.img.320.high.jpg/1732297881719.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_afb4/image.img.480.high.jpg/1732297881719.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_afb4/image.img.320.medium.jpg/1732297881719.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_afb4/image.img.620.high.jpg/1732297881719.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_afb4/image.img.320.low.jpg/1732297881719.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_afb4/image.img.480.medium.jpg/1732297881719.jpg" alt='decorative image' title='Tumoroids'></picture></div><style> #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystextimage_afb4 { max-width: 100%; } #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystextimage_afb4 img { width: 100%; max-width: 426px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><h3><a href="/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-cell-lines.html">OncoPro Tumoroid Cell Lines</a></h3><p>Explore our patient-derived cancer organoid cell lines, fully characterized and available for purchase.</p></div></div><div class="clearfix"></div></div></div><div container-id="cq-colctrl-lt0" comp-id="B2"><div class="textimage parbase section"><div class="image" id="cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystextimage_2b53"><a href="https://tfs.docebosaas.com/gsstraining/learn/course/5047/oncopro-tumoroid-culture-medium?generated_by=56741&hash=957509cd76014c21a204bb299a8351606c2fac36" target="_blank"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_2b53/image.img.full.high.jpg/1732297881751.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_2b53/image.img.full.high.jpg/1732297881751.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_2b53/image.img.320.high.jpg/1732297881751.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_2b53/image.img.480.high.jpg/1732297881751.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_2b53/image.img.320.medium.jpg/1732297881751.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_2b53/image.img.620.high.jpg/1732297881751.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_2b53/image.img.320.low.jpg/1732297881751.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_2b53/image.img.480.medium.jpg/1732297881751.jpg" alt='Microscopic view of tumoroid' title='Tumoroid eLearn'></picture></div><style> #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystextimage_2b53 { max-width: 100%; } #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystextimage_2b53 img { width: 100%; max-width: 426px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><h3><a adhocenable="false" href="https://tfs.docebosaas.com/gsstraining/learn/course/5047/oncopro-tumoroid-culture-medium?generated_by=56741&hash=957509cd76014c21a204bb299a8351606c2fac36" target="_blank">Tumoroid eLearn</a></h3><p>Review this interactive, online learning tool for a look into tumoroids and culture methods.</p></div></div><div class="clearfix"></div></div></div></div></div><div style="clear:both"></div><div class="heading section"><h2><br>Discover other products to support culture of tumoroids </h2></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="textimage parbase textimage-top section"><div class="image" id="cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystextimage_3078"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_3078/image.img.full.high.jpg/1732297881794.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_3078/image.img.full.high.jpg/1732297881794.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_3078/image.img.320.high.jpg/1732297881794.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_3078/image.img.480.high.jpg/1732297881794.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_3078/image.img.320.medium.jpg/1732297881794.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_3078/image.img.620.high.jpg/1732297881794.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_3078/image.img.320.low.jpg/1732297881794.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_3078/image.img.480.medium.jpg/1732297881794.jpg" alt='Cell culture plastics family image' title='Nunc plastics'></picture></div><style> #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystextimage_3078 { max-width: 100%; } #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystextimage_3078 img { width: 100%; max-width: 426px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><h3><a adhocenable="false" href="/sg/en/home/life-science/cell-culture/cell-culture-plastics.html">Nunc Cell Culture Plastics</a></h3><p>Thermo Scientific Nunc cell culture plastics offer non-treated flasks to support tumoroid culture.</p></div></div><div class="clearfix"></div></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="textimage parbase textimage-top section"><div class="image" id="cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystextimage_1f55"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_1f55/image.img.full.high.jpg/1732297881832.jpg' media="(min-width: 1px)"><img class='textimage-auto' srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_1f55/image.img.full.high.jpg/1732297881832.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_1f55/image.img.320.high.jpg/1732297881832.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_1f55/image.img.480.high.jpg/1732297881832.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_1f55/image.img.320.medium.jpg/1732297881832.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_1f55/image.img.620.high.jpg/1732297881832.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_1f55/image.img.320.low.jpg/1732297881832.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cancer-cell-culture/tumoroid-culture/tumoroid-medium/jcr:content/MainParsys/textimage_1f55/image.img.480.medium.jpg/1732297881832.jpg" alt='A bottle of HPLM with a graphic rendering of a cell' title='Gibco HPLM'></picture></div><style> #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystextimage_1f55 { max-width: 100%; } #cq-textimage-jsp-5b206439-5546-4669-9a5e-dca44a4c347dMainParsystextimage_1f55 img { width: 100%; max-width: 426px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><h3><a adhocenable="false" href="/sg/en/home/life-science/cell-culture/mammalian-cell-culture/cell-culture-media/human-plasma-like-medium.html">Gibco HPLM</a></h3><p>Human plasma-like medium is a physiologically relevant medium useful for downstream assays.</p></div></div><div class="clearfix"></div></div></div></div></div><div style="clear:both"></div><div class="divider section"><hr /></div><div class="well-cmp section"><div class="well well-gray-lighter well-default"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="text parbase section"><h3><a id="faq"></a><a href="/search/results?query=oncopro&persona=DocSupport&refinementAction=true&filter=document.result_type_s:Product%20FAQs" class="cta-link">See OncoPro Medium FAQs</a></h3></div></div></div></div></div></div><div class="divider section"><hr /></div><div class="reference parbase section"><div class="cq-dd-paragraph"><div class="text_72be text parbase"><p>For Research Use Only. 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