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</a></div></div> <div class="slideshow-controls"> <div class="slideshow-controls__arrows"></div> </div> <span class="product-label-list"><script id="variant-label-8029677289640-45599329878184" type="text/template"></script><div class="product-label-container"></div></span> </div> <div class="title-row" > <h1 class="title">Metabolic stability and metabolite identification & profiling</h1> </div> <div class="" > <div class="product-description rte cf"><div id="formula-bar-name-box-wrapper" class="name-box-enabled formula-bar-with-name-box-wrapper" data-mce-fragment="1"> <div id="formula-bar" class="formula-bar formulabar-disabled" data-mce-fragment="1"> <div id="t-formula-bar-input-container" data-mce-fragment="1"> <div dir="ltr" data-mce-fragment="1"> <div spellcheck="false" id="t-formula-bar-input" data-mce-fragment="1"> <div class="cell-input disabled-input" role="combobox" dir="ltr" aria-autocomplete="list" tabindex="0" data-mce-fragment="1" data-mce-tabindex="0" contenteditable="false" docs-unhandledkeys=""> <span data-mce-fragment="1">The compound is incubated with liver microsomes/liver S9 fraction/hepatocytes/plasma, and collected samples are analyzed by UPLC/HR-MS to measure stability of the compound and to evaluate metabolite profiles.</span><br data-mce-fragment="1"> </div> </div> </div> </div> </div> </div></div> </div> <div class="product-detail-accordion not-in-quickbuy" > <div class="cc-accordion" data-allow-multi-open="true"> <details class="cc-accordion-item is-open" open> <summary class="cc-accordion-item__title"><div class="with-icon"> <span class="material-symbols-outlined"> check </span> <span class="with-icon__beside">Protocol</span> </div></summary> <div class="cc-accordion-item__panel"> <div class="cc-accordion-item__content rte cf"><p>Test concentrations: 1</p><p>Time points: 5</p><p>Replicates: 2</p><p>Positive control</p><p>UPLC/HR-MS analysis</p></div> </div> </details> </div> </div> <div class="product-detail-accordion not-in-quickbuy" > <div class="cc-accordion" data-allow-multi-open="true"> <details class="cc-accordion-item is-open" open> <summary class="cc-accordion-item__title"><div class="with-icon"> <span class="material-symbols-outlined"> check </span> <span class="with-icon__beside">Deliverables</span> </div></summary> <div class="cc-accordion-item__panel"> <div class="cc-accordion-item__content rte cf"><p><span class="metafield-multi_line_text_field">Intrinsic clearance<br /> <br /> Half-life<br /> <br /> Metabolite identifications<br /> <br /> Metabolite profiles</span></p></div> </div> </details> </div> </div> <div class="product-detail-accordion not-in-quickbuy" > <div class="cc-accordion" data-allow-multi-open="true"> <details class="cc-accordion-item is-open" open> <summary class="cc-accordion-item__title"><div class="with-icon"> <span class="material-symbols-outlined"> check </span> <span class="with-icon__beside">Reporting</span> </div></summary> <div class="cc-accordion-item__panel"> <div class="cc-accordion-item__content rte cf"><p><span class="metafield-multi_line_text_field">Excel report<br /> (full report available per request)</span></p></div> </div> </details> </div> </div> <div class="product-detail-accordion not-in-quickbuy" > <div class="cc-accordion" data-allow-multi-open="true"> <details class="cc-accordion-item"> <summary class="cc-accordion-item__title">Background</summary> <div class="cc-accordion-item__panel"> <div class="cc-accordion-item__content rte cf"><p>Measuring the metabolic stability of a compound in vitro provides an estimate regarding its stability, and thus elimination rate by metabolism in the body. Hepatic clearance is the most important parameter when assessing metabolic stability, and the most common approaches for its prediction are in vitro assays using liver microsomes or hepatocytes; the former focusing mainly on oxidative (CYP-mediated) metabolism, and the latter also on conjugative drug metabolism. Metabolite identification and profiling are relevant in several stages of drug discovery & development. Optimization of metabolic clearance requires information on the main metabolically labile “soft-spots” and their further chemical modification. Selection of suitable animal species for toxicity studies is facilitated by the prediction of species with most similar metabolite profiles with respect to human (first in vitro and then in vivo). The correct evaluation of metabolic enzymes involved in clearance also requires identification of the metabolites, and eventually the metabolic clearance routes in human in vivo has to be characterized in a detailed level.</p></div> </div> </details> </div> </div> </div> </div> <div class="detail product-column-right" data-cc-animate data-cc-animate-delay="0.2s"> <div class="product-form" data-ajax-add-to-cart="true" data-product-id="8029677289640" data-enable-history-state="true"> <div class="buy-buttons-row" > <form method="post" action="/cart/add" id="product-form-template--16460963283112__main-8029677289640" accept-charset="UTF-8" class="form product-purchase-form" enctype="multipart/form-data" data-product-id="8029677289640"><input type="hidden" name="form_type" value="product" /><input type="hidden" name="utf8" value="✓" /> <select name="id" class="original-selector hidden" aria-label="Options"> <option value="45599329878184" selected="selected" data-stock="">Default Title</option> </select> <div class="quantity-submit-row input-row "> <div class="quantity-submit-row__submit input-row"><button class="button button--large" type="submit" data-add-to-cart-text="Add to quote request">Add to quote request</button> </div> </div> <input type="hidden" name="product-id" value="8029677289640" /><input type="hidden" name="section-id" value="template--16460963283112__main" /></form> </div> <div class="lightish-spaced-row-above only-in-quickbuy"> <a class="more" href="/products/metabolic-stability-and-metabolite-identification-profiling"> <span class="beside-svg underline">More details</span> <span class="icon--small icon-natcol ltr-icon"> <span class="material-symbols-outlined"> chevron_right </span></span> </a> </div> </div> <script type="application/json" id="ProductJson-8029677289640">{"id":8029677289640,"title":"Metabolic stability and metabolite identification \u0026 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to evaluate metabolite profiles.\u003c\/span\u003e\u003cbr data-mce-fragment=\"1\"\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","published_at":"2023-11-06T16:37:33+02:00","created_at":"2023-11-06T16:37:33+02:00","vendor":"Admescope","type":"","tags":[],"price":0,"price_min":0,"price_max":0,"available":true,"price_varies":false,"compare_at_price":null,"compare_at_price_min":0,"compare_at_price_max":0,"compare_at_price_varies":false,"variants":[{"id":45599329878184,"title":"Default Title","option1":"Default Title","option2":null,"option3":null,"sku":"","requires_shipping":false,"taxable":true,"featured_image":null,"available":true,"name":"Metabolic stability and metabolite identification \u0026 profiling","public_title":null,"options":["Default Title"],"price":0,"weight":0,"compare_at_price":null,"inventory_management":null,"barcode":"","requires_selling_plan":false,"selling_plan_allocations":[]}],"images":["\/\/admeshop.com\/cdn\/shop\/files\/InvitrometabolismNEG_daae9e5c-12f0-488a-9c58-f5f4711bc801.png?v=1702464109"],"featured_image":"\/\/admeshop.com\/cdn\/shop\/files\/InvitrometabolismNEG_daae9e5c-12f0-488a-9c58-f5f4711bc801.png?v=1702464109","options":["Title"],"media":[{"alt":null,"id":29795125559464,"position":1,"preview_image":{"aspect_ratio":1.0,"height":1299,"width":1299,"src":"\/\/admeshop.com\/cdn\/shop\/files\/InvitrometabolismNEG_daae9e5c-12f0-488a-9c58-f5f4711bc801.png?v=1702464109"},"aspect_ratio":1.0,"height":1299,"media_type":"image","src":"\/\/admeshop.com\/cdn\/shop\/files\/InvitrometabolismNEG_daae9e5c-12f0-488a-9c58-f5f4711bc801.png?v=1702464109","width":1299}],"requires_selling_plan":false,"selling_plan_groups":[],"content":"\u003cdiv id=\"formula-bar-name-box-wrapper\" class=\"name-box-enabled 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